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1. Lasso Peptide Benenodin-1 Is a Thermally Actuated [1]Rotaxane Switch

Author:
Zong, Chuhan; Wu, Michelle J.; Qin, Jason Z.; Link, A. James
Source:
Journal of the American Chemical Society 2017 v.139 no.30 pp. 10403-10409
ISSN:
1520-5126
Subject:
amino acid substitution,   etc   ; enzymes; heat; peptides;     Show all 4 Subjects
Abstract:
... Mechanically interlocked molecules that change their conformation in response to stimuli have been developed by synthetic chemists as building blocks for molecular machines. Here we describe a natural product, the lasso peptide benenodin-1, which exhibits conformational switching between two distinct threaded conformers upon actuation by heat. We have determined the structures of both conformers a ...
DOI:
10.1021/jacs.7b04830

2. Characterization of a Pestivirus H isolate originating from goats

Author:
Shi, Hongfei; Leng, Chaoliang; Xu, Qian; Shi, Hongling; Sun, Shiyu; Kan, Yunchao; Yao, Lunguang
Source:
Virus genes 2018 v.54 no.4 pp. 603-607
ISSN:
0920-8569
Subject:
amino acid substitution,   etc   ; Pestivirus; cattle; genome; goats; nucleotide sequences; nucleotides; phylogeny; Italy;     Show all 9 Subjects
Abstract:
... Natural Pestivirus H infections in cattle have been reported worldwide; however, only a few cases of Pestivirus H have been described in non-bovine ruminants such as goats. A new Pestivirus H HN1507 strain was isolated from an infected goat in 2015 and the genome sequence was determined. The full-length genome sequence was 12,556 nucleotides. Phylogenetic analysis, based on the complete genome and ...
DOI:
10.1007/s11262-018-1579-7

3. Surface engineering of polyester-degrading enzymes to improve efficiency and tune specificity

Author:
Biundo, Antonino; Ribitsch, Doris; Guebitz, GeorgM.
Source:
Applied microbiology and biotechnology 2018 v.102 no.8 pp. 3551-3559
ISSN:
0175-7598
Subject:
amino acid substitution,   etc   ; carboxylesterase; engineering; hydrolysis; hydrophobicity; polyesters; site-directed mutagenesis; sorption;     Show all 8 Subjects
Abstract:
... Certain members of the carboxylesterase superfamily can act at the interface between water and water-insoluble substrates. However, nonnatural bulky polyesters usually are not efficiently hydrolyzed. In the recent years, the potential of enzyme engineering to improve hydrolysis of synthetic polyesters has been demonstrated. Regions on the enzyme surface have been modified by using site-directed mu ...
DOI:
10.1007/s00253-018-8850-7

4. Goats singly heterozygous for PRNP S146 or K222 orally inoculated with classical scrapie at birth show no disease at ages well beyond 6 years

Author:
Cinar, M.U.; Schneider, D.A.; Waldron, D.F.; O’Rourke, K.I.; White, S.N.
Source:
The veterinary journal 2018 v.233 pp. 19-24
ISSN:
1090-0233
Subject:
amino acid substitution,   etc   ; alleles; biopsy; genetic resistance; goats; heterozygosity; homozygosity; prions; scrapie; sheep;     Show all 10 Subjects
Abstract:
... Scrapie is a transmissible spongiform encephalopathy of sheep and goats, and scrapie eradication programs in many parts of the world rely on strong genetic resistance to classical scrapie in sheep. However, the utility of putative resistance alleles in goats has been a focus of research because goats can transmit scrapie to sheep and may serve as a scrapie reservoir. Prior work showed that disease ...
DOI:
10.1016/j.tvjl.2017.12.019

5. Vaccine-mismatched influenza B/Yamagata lineage viruses in Cuba, 2012–2013 season

Author:
Arencibia, Amely; Piñón, Alexander; Acosta, Belsy; Fernandez, Leandro; Muné, Mayra; Valdés, Odalys; Savón, Clara; Oropesa, Suset; Gonzalez, Grehete; Roque, Rosmery; Gonzalez, Guelsys; Hernández, Bárbara; Alfonso, Javier Martínez
Source:
Infection, genetics, and evolution 2018 v.58 pp. 110-114
ISSN:
1567-1348
Subject:
amino acid substitution,   etc   ; Influenza B virus; disease outbreaks; genes; influenza; influenza vaccines; monitoring; viruses; Cuba;     Show all 9 Subjects
Abstract:
... Annual trivalent influenza vaccines contain one of influenza B lineages; influenza B/Victoria-lineage or influenza B/Yamagata viruses. Theoretically, these vaccines should protect against viruses expected to circulate in the next influenza season. The National Influenza Centers, based on surveillance data from National Reference Laboratories, selects the strains composing each annual trivalent or ...
DOI:
10.1016/j.meegid.2017.12.004

6. Analysis of the glycoproteins of Seoul orthohantavirus strain B1 associated with fusion activity

Author:
Isegawa, Yuji; Okuno, Yoshinobu
Source:
Archives of virology 2018 v.163 no.2 pp. 419-425
ISSN:
0304-8608
Subject:
amino acid substitution,   etc   ; cell fusion; genes; giant cells; glycoproteins; pH; viruses;     Show all 7 Subjects
Abstract:
... We analyzed two virus variants (S1 and L1) from Seoul orthohantavirus strain B1. Strain B1 produces large opaque plaques when plated on Vero E6 cell monolayers. However, although the L1 variant produced the large opaque plaques common to the strain, the variant S1 produced small clear ones on Vero E6 cells. Five days after Vero E6 cells were infected with the S1 variant, polykaryons formed spontan ...
DOI:
10.1007/s00705-017-3623-7

7. PB2 and HA mutations increase the virulence of highly pathogenic H5N5 clade 2.3.4.4 avian influenza virus in mice

Author:
Yu, Zhijun; Cheng, Kaihui; Sun, Weiyang; Zhang, Xinghai; Xia, Xianzhu; Gao, Yuwei
Source:
Archives of virology 2018 v.163 no.2 pp. 401-410
ISSN:
0304-8608
Subject:
amino acid substitution,   etc   ; Influenza A virus; hemagglutinins; lethal dose; mice; monitoring; poultry; virulence; virus replication; viruses;     Show all 10 Subjects
Abstract:
... H5 clade 2.3.4.4 influenza A viruses pose a potential threat to public health and are a cause of public concern. Here, we generated mouse-adapted viruses of a waterfowl-origin H5N5 virus (H5 clade 2.3.4.4) to identify adaptive changes that confer increased virulence in mammals. After two passages, we obtained a mouse-adapted H5N5 virus that contained single amino acid substitutions in the PB2 (E62 ...
DOI:
10.1007/s00705-017-3631-7

8. Characterization and mutation analysis of a halotolerant serine protease from a new isolate of Bacillus subtilis

Author:
Takenaka, Shinji; Yoshinami, Jyun; Kuntiya, Ampin; Techapun, Charin; Leksawasdi, Noppol; Seesuriyachan, Phisit; Chaiyaso, Thanongsak; Watanabe, Masanori; Tanaka, Kosei; Yoshida, Ken-ichi
Source:
Biotechnology letters 2018 v.40 no.1 pp. 189-196
ISSN:
0141-5492
Subject:
amino acid substitution,   etc   ; Bacillus subtilis; amino acids; mutants; protein engineering; salt tolerance; sodium chloride; subtilisin;     Show all 8 Subjects
Abstract:
... OBJECTIVES: A bacterial halotolerant enzyme was characterized to understand the molecular mechanism of salt adaptation and to explore its protein engineering potential. RESULTS: Halotolerant serine protease (Apr_No16) from a newly isolated Bacillus subtilis strain no. 16 was characterized. Multiple alignments with previously reported non-halotolerant proteases, including subtilisin Carlsberg, indi ...
DOI:
10.1007/s10529-017-2459-2

9. Design and characterization of new β-glucuronidase active site variants with altered substrate specificity

Author:
Li, Ye; Wang, Zhiqing; Cirino, PatrickC.
Source:
Biotechnology letters 2018 v.40 no.1 pp. 111-118
ISSN:
0141-5492
Subject:
amino acid substitution,   etc   ; Escherichia coli; active sites; beta-glucuronidase; data collection; enzyme activity; models; substrate specificity;     Show all 8 Subjects
Abstract:
... OBJECTIVE: To isolate and characterize the kinetics of variants of E. coli β-glucuronidase (GUS) having altered substrate specificity. RESULTS: Two small combinatorial libraries of E. coli GUS variants were constructed and screened for improved activities towards the substrate p-nitrophenyl-β-D-galactoside (pNP-gal). Nine of the most active variants were purified and their kinetic parameters were ...
DOI:
10.1007/s10529-017-2447-6

10. Combining Experimental and Simulation Techniques to Understand Morphology Control in Pentapeptide Nanostructures B

Author:
Mishra, Narendra Kumar; Jain, Alok; Peter, Christine; Verma, Sandeep
Source:
The Journal of physical chemistry 2017 v.121 no.34 pp. 8155-8161
ISSN:
1520-5207
Subject:
amino acid substitution,   etc   ; dimerization; drugs; hydrogen bonding; nanomaterials; tissue engineering;     Show all 6 Subjects
Abstract:
... A controlled route toward biocompatible nanostructures has immense relevance for drug delivery and tissue engineering. We present an experimental–computational study identifying factors that govern the formation of well-defined aggregates by self-assembled pentapeptides, using single amino acid substitution. A subtle interplay between peptide rigidity/flexibility, hydrogen-bonding capacity, partit ...
DOI:
10.1021/acs.jpcb.7b06005

11. Coelacanth-specific adaptive genes give insights into primitive evolution for water-to-land transition of tetrapods

Author:
Lee, Chul; Jeong, Heesu; Yoo, DongAhn; Kim, Eun Bae; Nam, Bo-Hye; Kim, Heebal
Source:
Marine genomics 2018 v.38 pp. 89-95
ISSN:
1874-7787
Subject:
amino acid substitution,   etc   ; Holostei; Sarcopterygii; Teleostei; evolution; femur; fins; fish; homeotic genes; humerus; learning; nitrogen metabolism; phenotype;     Show all 13 Subjects
Abstract:
... Coelacanth is a group of extant lobe-finned fishes in Sarcopterygii that provides evolutionary information for the missing link between ray-finned fish and tetrapod vertebrates. Its phenotypes, different from actinopterygian fishes, have been considered as primitive terrestrial traits such as cartilages in their fatty fins which are homologous with the humerus and femur. To investigate molecular e ...
DOI:
10.1016/j.margen.2017.12.004

12. Mutational analysis of TAF6 revealed the essential requirement of the histone‐fold domain and the HEAT repeat domain for transcriptional activation

Author:
Dahiya, Rashmi; Natarajan, Krishnamurthy
Source:
The FEBS journal 2018 v.285 no.8 pp. 1491-1510
ISSN:
1742-464X
Subject:
amino acid substitution,   etc   ; dimerization; gene overexpression; histones; mutants; mutational analysis; phenotype; precipitin tests; recombinant proteins; transcription (genetics); transcriptional activation; yeasts;     Show all 12 Subjects
Abstract:
... TAF6, bearing the histone H4‐like histone‐fold domain (HFD), is a subunit of the core TAF module in TFIID and SAGA transcriptional regulatory complexes. We isolated and characterized several yeast TAF6 mutants bearing amino acid substitutions in the HFD, the middle region or the HEAT repeat domain. The TAF6 mutants were highly defective for transcriptional activation by the Gcn4 and Gal4 activator ...
DOI:
10.1111/febs.14423

13. Structural evidence for a fatty acid-independent myotoxic mechanism for a phospholipase A2-like toxin Proteins and proteomics

Author:
Salvador, Guilherme H.M.; dos Santos, Juliana I.; Borges, Rafael J.; Fontes, Marcos R.M.
Source:
Biochimica et biophysica acta 2018 v.1866 no.3 pp. 473-481
ISSN:
1570-9639
Subject:
amino acid substitution,   etc   ; Bothrops; crystal structure; fatty acids; hydrogen bonding; ligands; mechanism of action; molecular dynamics; phospholipases; simulation models; toxins;     Show all 11 Subjects
Abstract:
... The myotoxic mechanism for PLA2-like toxins has been proposed recently to be initiated by an allosteric change induced by a fatty acid binding to the protein, leading to the alignment of the membrane docking site (MDoS) and membrane disrupting site (MDiS). Previous structural studies performed by us demonstrated that MjTX-II, a PLA2-like toxin isolated from Bothrops moojeni, presents a different m ...
DOI:
10.1016/j.bbapap.2017.12.008

14. Familial Congenital Methemoglobinemia in Pomeranian Dogs Caused by a Missense Variant in the NADH‐Cytochrome B5 Reductase Gene

Author:
Shino, H.; Otsuka‐Yamasaki, Y.; Sato, T.; Ooi, K.; Inanami, O.; Sato, R.; Yamasaki, M.
Source:
Journal of veterinary internal medicine 2018 v.32 no.1 pp. 165-171
ISSN:
0891-6640
Subject:
amino acid substitution,   etc   ; Beagle; Canidae; dogs; erythrocytes; etiology; genes; glutathione; isoleucine; leucine; methemoglobinemia; sequence analysis; turbidity; veterinary medicine;     Show all 14 Subjects
Abstract:
... BACKGROUND: In veterinary medicine, congenital methemoglobinemia associated with nicotinamide adenine dinucleotide (NADH)‐cytochrome b5 reductase (b5R) deficiency is rare. It has been reported in several breeds of dogs, but little information is available about its etiology. OBJECTIVES: To analyze the NADH‐cytochrome b5 reductase gene, CYB5R3, in a Pomeranian dog family with methemoglobinemia susp ...
DOI:
10.1111/jvim.15031

15. Tracing the evolution of venom phospholipases A2 in Gloydius strauchii and related pitvipers: A tale of two acidic isozymes

Author:
Yang, Zhang-min; Xie, Ying-zhe; Yan, Su-xian; Liu, Zhen-zhen; Song, Jian-cheng; Wang, Zhe-zhi; Tsai, Inn-Ho
Source:
Toxicon 2018 v.141 pp. 65-72
ISSN:
0041-0101
Subject:
amino acid substitution,   etc   ; Gloydius; amino acid sequences; anticoagulants; evolution; genes; isoelectric point; isozymes; neurotoxicity; phospholipase A2; phospholipases; snake venoms;     Show all 12 Subjects
Abstract:
... Two acidic Asp⁴⁹-PLA2s with Glu⁶ substitution and a neutral Lys⁴⁹-PLA (designated Gst-K49) were cloned from G. strauchii venom glands, their full amino acid sequences were deduced. The predominant acidic PLA2 (designated Gst-E6a) contains 124 residues and the M¹⁸W³⁰ substitutions, while the minor acidic PLA2 (designated Gst-E6b) contains 122 residues and the V¹⁸A³⁰ substitutions. Their sequences a ...
DOI:
10.1016/j.toxicon.2017.11.010

16. Contingency and entrenchment in protein evolution under purifying selection

Author:
Shah, Premal; McCandlish, David M.; Plotkin, Joshua B.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2015 v.112 no.25 pp. E3226-E3235
ISSN:
0027-8424
Subject:
amino acid substitution,   etc   ; evolution; proteins;     Show all 3 Subjects
Abstract:
... Significance How large a role does history play in evolution? Do later events depend critically on specific earlier events, or do all events occur more or less independently? If a change occurs early in evolution, does it become easier or harder to revert the change as time proceeds? Here, we explore these ideas in the context of protein evolution, by simulating sequence evolution under purifying ...
DOI:
10.1073/pnas.1412933112

17. A 3-year follow-up study after treatment with simeprevir in combination with pegylated interferon-α and ribavirin for chronic hepatitis C virus infection

Author:
Zoulim, Fabien; Moreno, Christophe; Lee, Samuel S.; Buggisch, Peter; Horban, Andrzej; Lawitz, Eric; Corbett, Chris; Lenz, Oliver; Fevery, Bart; Verbinnen, Thierry; Shukla, Umesh; Jessner, Wolfgang
Source:
Virology journal 2018 v.15 no.1 pp. 26-26
ISSN:
1743-422X
Subject:
amino acid substitution,   etc   ; European Union; Hepatitis C virus; RNA; chronic hepatitis C; durability; genotype; interferon-alpha; patients; proteinase inhibitors; United States;     Show all 11 Subjects
Abstract:
... BACKGROUND: Simeprevir is approved with pegylated interferon and ribavirin (PR) for chronic hepatitis C virus (HCV) genotype (GT) 1 and GT4 infection in the USA and the European Union. METHODS: This 3-year follow-up study assessed the durability of sustained virologic response (SVR) (undetectable HCV RNA 12 or 24 weeks after treatment end), and evaluated the persistence of treatment-emergent NS3/4 ...
DOI:
10.1186/s12985-018-0936-4

18. First survey on association of TMEM154 and CCR5 variants with serological maedi-visna status of sheep in German flocks

Author:
Molaee, Vahid; Eltanany, Marwa; Lühken, Gesine
Source:
Veterinary research 2018 v.49 no.1 pp. 36-36
ISSN:
1297-9716
Subject:
amino acid substitution,   etc   ; CCR5 receptor; Lentivirus; Texel; breeding; chemokines; enzyme-linked immunosorbent assay; flocks; genes; genotyping; polymerase chain reaction; proviruses; sheep; surveys; transmembrane proteins; veterinary medicine; Germany;     Show all 17 Subjects
Abstract:
... Maedi-visna, a disease caused by small ruminant lentiviruses (SRLVs), is present in sheep from many countries, also including Germany. An amino acid substitution (E/K) at position 35 of the transmembrane protein 154 (TMEM154) as well as a deletion in the chemokine (C-C motif) receptor type 5 gene (CCR5) were reported to be associated with the serological MV status and/or the SRLV provirus concentr ...
DOI:
10.1186/s13567-018-0533-y

19. Significant increase in toxicity of the Cry1Ac1 protein of Bacillus thuringiensis by the exchange of two amino acids in its domain III

Author:
Reinoso-Pozo, Yaritza; Del Rincón-Castro, Ma. Cristina; Ibarra, Jorge E.
Source:
Biological control 2018 v.125 pp. 1-6
ISSN:
1049-9644
Subject:
amino acid substitution,   etc   ; Bacillus thuringiensis; Manduca sexta; Trichoplusia ni; amino acids; biological control; crystal proteins; crystals; genetic similarity; insecticidal proteins; larvae; mutants; site-directed mutagenesis; soil; toxicity; toxins;     Show all 16 Subjects
Abstract:
... The LBIT-1200 strain of Bacillus thuringiensis was recently isolated from soil, and showed high biochemical and genetic similarities with the HD-73 standard strain, besides expressing only a Cry1Ac protein: Cry1Ac39. However, LBIT-1200 was 6.4 and 9.5 times more toxic against Manduca sexta and Trichoplusia ni larvae, respectively, than HD-73, which expresses the Cry1Ac1 protein. Sequencing of Cry1 ...
DOI:
10.1016/j.biocontrol.2018.06.006

20. Characterization of a Novel Semi-dwarf GID1 Allele Identifies an Amino Acid Required for Its Interaction with SLR1 in Rice

Author:
Wang, Minxia; Qin, Yanling; Chun, Yan; Zhao, Jinfeng; Fang, Jingjing; Zafar, Syed Adeel; Guo, Baotai; Li, Xueyong
Source:
Journal of plant growth regulation 2018 v.37 no.3 pp. 840-848
ISSN:
0721-7595
Subject:
amino acid substitution,   etc   ; Oryza sativa; alleles; gibberellins; growth and development; internodes; leucine; mutants; phenotype; plant growth; plant hormones; proline; rice; signal transduction;     Show all 14 Subjects
Abstract:
... Gibberellins (GAs) are a group of endogenous phytohormones, playing important roles in plant growth and development. GA-induced interaction between the receptor GIBBERELLIN INSENSITIVE DWARF1 (GID1) and the DELLA repressor protein SLENDER RICE1 (SLR1) is critical to release GA signaling. In this study, a semi-dwarf mutant s1-1 was isolated from the rice variety Nipponbare (Oryza sativa, japonica) ...
DOI:
10.1007/s00344-018-9790-2