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aminoacidsubstitution, etc ; Escherichia coli; beta-lactamase; mutants; Show all 4 Subjects
Abstract:
... Aztreonam/avibactam is a promising antimicrobial combination with additional coverage for metallo-β-lactamases compared with ceftazidime/avibactam. A carbapenem-resistant blaKPC₋₂-carrying Escherichia coli clinical isolate had four extra amino acids in penicillin-binding protein 3 (PBP3), which has been known to mediate reduced susceptibility to aztreonam/avibactam. This prompted us to investigate ...
aminoacidsubstitution, etc ; ancestry; biotechnology; hydrolases; hydrolysates; mutagenesis; oligosaccharides; Show all 7 Subjects
Abstract:
... Engineering of hydrolases to shift their hydrolysate types has not been attempted so far, though computer-assisted enzyme design has been successful. A novel integrative strategy for engineering and screening the β-1,3-xylanase with desired hydrolysate types was proposed, with the purpose to solve problems that the separation and preparation of β-1,3-xylo-oligosaccharides was in high cost yet in l ...
aminoacidsubstitution, etc ; Japan; epidemiology; humans; immunology; Europe; India; Show all 7 Subjects
Abstract:
... CTX-M ESBL are widely found in animal and human infections. For better understanding of CTX-M variations and epidemiology, a total of 2210 CTX-M sequences were retrieved from NCBI as at 20 December 2020. The maximum incidences of CTX-M were reported in China (n = 508), USA (n = 354) and Japan (n = 180). Single amino acid substitution in the domain region of CTX-M ESBL lead to survival benefits to ...
aminoacidsubstitution, etc ; agronomy; alleles; amino acid composition; barley; cultivars; drought; introns; water stress; Show all 9 Subjects
Abstract:
... β-amylase activity is related to the polymorphisms of Bmy1 intron III; however, no attention has been given to such relationships under environmental stresses such as drought. In this study, 73 cultivated barley genotypes and 52 Tibetan wild barley accessions were used to test the association between Bmy1 gene intron III polymorphisms and β-amylase activity under drought stress. Our results showed ...
aminoacidsubstitution, etc ; ABC transporters; Bombyx mori; crosslinking; cysteine; mutants; surface plasmon resonance; toxins; Show all 8 Subjects
Abstract:
... Little information is available regarding the region of Cry toxins involved in binding to their major receptors, the ATP‐binding cassette (ABC) transporters. We analyzed which Cry1Aa amino acid residues contribute to binding to Bombyx mori ABC transporter C2 (BmABCC2). Several two oxidized double‐cysteine substitution mutant toxins were made. In these, two amino acids at distant positions on toxin ...
aminoacidsubstitution, etc ; Aspergillus fumigatus; antifungal agents; aspergillosis; azoles; genes; heart transplant; kidneys; mycology; patients; Show all 10 Subjects
Abstract:
... We present a case of a 55-year-old man with a heart transplant who acquired Invasive Aspergillosis by Aspergillus fumigatus with the focus in the kidney. During about two years of antifungal treatment, most of the time with voriconazole, it was possible to obtain nine isolates of A. fumigatus, with the same genotypic characteristics, but with an increase in MIC for several azoles. The two last iso ...
aminoacidsubstitution, etc ; CD4-positive T-lymphocytes; autoimmunity; hydrogen; ligands; molecular dynamics; monoclonal antibodies; peptides; Show all 8 Subjects
Abstract:
... In the process of structure-function studies on the MHC class II molecule expressed in autoimmunity prone SJL mice, I-Aˢ, we discovered a disparity from the reported sequence of the MHC class II beta chain. The variant is localized at a highly conserved site of the beta chain, at residue 58. Our studies revealed that this single amino acid substitution of Pro for Ala at this residue, found in I-Aˢ ...
aminoacidsubstitution, etc ; Plasmodium falciparum; amino acids; aminoacyl tRNA ligases; aminoacylation; enzyme activity; esterification; parasitology; Show all 8 Subjects
Abstract:
... The specificity of each aminoacyl-tRNA synthetase (aaRS) for its cognate amino acid ensures correct tRNA esterification and allows fidelity in protein synthesis. The aaRSs discriminate based on the chemical properties of their amino acid substrates and structural features of the binding pockets. In this study, we characterized aspartyl-(DRS) and asparaginyl-tRNA synthetase (NRS) from Plasmodium fa ...
aminoacidsubstitution, etc ; Vibrio cholerae; guanosinetriphosphatase; microbiology; mutants; statistical analysis; stress response; transmission electron microscopy; Show all 8 Subjects
Abstract:
... CgtA, a highly conserved 50S ribosome-associated essential GTPase, acts as a repressor of the stringent stress response under nutrient-rich growth conditions to suppress basal levels of the alarmone ppGpp in V. cholerae. To further explore the in vivo functionality of CgtA, we introduced an amino acid substitution, i.e., Gly98Asp, in a conserved glycine residue in the N-terminal domain. The constr ...
aminoacidsubstitution, etc ; Magnaporthe oryzae; blast disease; cross resistance; cytochrome b; normal distribution; picoxystrobin; quinones; risk; Show all 9 Subjects
Abstract:
... BACKGROUND: Rice blast, caused by Magnaporthe oryzae, is a destructive disease threatening the production of staple foods worldwide. Quinone outside inhibitors (QoIs) are a group of chemicals exhibiting excellent activity against a majority of plant pathogens, with the disadvantage that pathogens can easily develop resistance to QoIs. RESULTS: Here, we investigated the activity of picoxystrobin ag ...
... The canonical function of lentiviral Vif proteins is to counteract the mutagenic potential of APOBEC3 antiviral restriction factors. However, recent studies have discovered that Vif proteins from diverse HIV-1 and simian immunodeficiency virus (SIV) isolates degrade cellular B56 phosphoregulators to remodel the host phosphoproteome and induce G2/M cell cycle arrest. Here, we evaluate the conservat ...
... BACKGROUND: NPHS2 is the causative gene of nephrotic syndrome type 2 (MIM 600995) which often clinically manifests as steroid-resistant nephrotic syndrome (SRNS). The NPHS2 gene encodes a slit diaphragm (SD) associated protein podocin. OBJECTIVE: This study reported a novel disease-causing mutation of NPHS2 in a Chinese family with SRNS. We also investigated the pathogenic mechanism of the variant ...
... Glycyrrhizin (GL) is known to exhibit a variety of useful pharmacological activities, including anti-inflammation, anti-hepatotoxicity, and enhancement of intestinal drug absorption. GL has been reported to modify the assembly of actin filaments, thereby modulating tight junction (TJ) integrity, but the detailed molecular mechanisms of this remain unclear. In this study, we first found that GL bin ...
aminoacidsubstitution, etc ; Arabidopsis; Caryophyllales; Fabaceae; Nicotiana benthamiana; active sites; betalains; essential amino acids; legumes; oxidoreductases; protein synthesis; tyrosine; Show all 12 Subjects
Abstract:
... l‐Tyrosine is an essential amino acid for protein synthesis and is also used in plants to synthesize diverse natural products. Plants primarily synthesize tyrosine via TyrA arogenate dehydrogenase (TyrAₐ or ADH), which are typically strongly feedback inhibited by tyrosine. However, two plant lineages, Fabaceae (legumes) and Caryophyllales, have TyrA enzymes that exhibit relaxed sensitivity to tyro ...
aminoacidsubstitution, etc ; body size; body weight; brain; cell growth; cell proliferation; genome; growth retardation; mutants; patients; phosphorylation; research; Show all 12 Subjects
Abstract:
... The adaptor protein GAREM has two subtypes. Each is involved in Erk activation signaling downstream of the cell growth factor receptor in cultured cells. Regarding their role in individual animals, we have previously reported that mice deficient in GAREM2, which is highly expressed in the brain, exhibit emotional changes. In this paper, we report an amino acid substitution mutation (K291R) in GARE ...
... BACKGROUND: Mitochondrial complex I deficiency (MCID) is the most common biochemical defect identified in childhood with mitochondrial diseases, mainly including Leigh syndrome, encephalopathy, macrocephaly with progressive leukodystrophy, hypertrophic cardiomyopathy and myopathy. OBJECTIVE: To identify genetic cause in a patient with early onset autosomal recessive MCID. METHODS: Trio whole-exome ...
aminoacidsubstitution, etc ; Rana chensinensis; antibacterial properties; atomic force microscopy; biotechnology; cell membranes; forests; frogs; hemolysis; hydrophilicity; hydrophobicity; peptides; surfactants; viability; Show all 14 Subjects
Abstract:
... Brevinin2-CE (B2CE), a natural peptide containing 37 amino acids, was first isolated from the skin secretions of the Chinese forest frog Rana chensinensis. B2CE shows good antibacterial activity. In this study, a series of B2CE analogs with differences in cationicity, α-helicity, hydrophobicity and amphipathic properties were designed through chain-length deletion and amino acid substitution. The ...
aminoacidsubstitution, etc ; Equidae; Influenza A virus; amino acids; antigenic variation; biosecurity; equine influenza; genes; glycosylation; hemagglutinins; horses; pathogens; phylogeny; vaccines; Show all 14 Subjects
Abstract:
... Equine influenza virus (EIV) is a major infectious pathogen causing significant respiratory signs in equids worldwide. Voluntary surveillances in the United States recently reported EIV detection in horses with respiratory signs even with adequate vaccine protocols and biosecurity programs and posed a concern about suboptimal effectiveness of EIV vaccine in the United States. This study aims to de ...
... Efflux pump of Major Facilitator Superfamily (MFS) is widely distributed in bacteria, while its role in regulating antibiotic resistance of nosocomial pathogen Klebsiella pneumoniae remains unclear. Herein we analyzed the effect of amino acid substitution of MFS efflux pump KmrA on its export efficiency via molecular biology and molecular dynamics (MD). After searching across the 804 sequenced K. ...
... Corynespora leaf spot caused by Corynespora cassiicola is an important foliar disease in cucumber. Succinate dehydrogenase inhibitors are the main fungicides used to control this disease. With the application of succinate dehydrogenase inhibitors (SDHIs) in the field, boscalid-resistant isolates have been continuously detected in the field. Resistance monitoring programs were performed to investig ...