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You searched for: Subject "amino acid substitution" Remove constraint Subject: "amino acid substitution"
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1. A previously unappreciated polymorphism in the beta chain of I-As expressed in autoimmunity-prone SJL mice: Combined impact on antibody, CD4 T cell recognition and MHC class II dimer structural stability

Author:
Katherine A. Richards; Courtney Lavery; Grant L.J. Keller; Jim Miller; Brian M. Baker; Andrea J. Sant
Source:
Molecular immunology 2022 v.143 pp. 17-26
ISSN:
0161-5890
Subject:
amino acid substitution,   etc   ; CD4-positive T-lymphocytes; autoimmunity; hydrogen; ligands; molecular dynamics; monoclonal antibodies; peptides;     Show all 8 Subjects
Abstract:
... In the process of structure-function studies on the MHC class II molecule expressed in autoimmunity prone SJL mice, I-Aˢ, we discovered a disparity from the reported sequence of the MHC class II beta chain. The variant is localized at a highly conserved site of the beta chain, at residue 58. Our studies revealed that this single amino acid substitution of Pro for Ala at this residue, found in I-Aˢ ...
DOI:
10.1016/j.molimm.2021.12.022

2. A single amino acid substitution alters activity and specificity in Plasmodium falciparum aspartyl & asparaginyl-tRNA synthetases

Author:
Vivek Kumar Sharma; Swati Gupta; Jyoti Chhibber-Goel; Manickam Yogavel; Amit Sharma
Source:
Molecular and biochemical parasitology 2022 v.250 pp. 111488
ISSN:
0166-6851
Subject:
amino acid substitution,   etc   ; Plasmodium falciparum; amino acids; aminoacyl tRNA ligases; aminoacylation; enzyme activity; esterification; parasitology;     Show all 8 Subjects
Abstract:
... The specificity of each aminoacyl-tRNA synthetase (aaRS) for its cognate amino acid ensures correct tRNA esterification and allows fidelity in protein synthesis. The aaRSs discriminate based on the chemical properties of their amino acid substrates and structural features of the binding pockets. In this study, we characterized aspartyl-(DRS) and asparaginyl-tRNA synthetase (NRS) from Plasmodium fa ...
DOI:
10.1016/j.molbiopara.2022.111488

3. Selection of Aspergillus fumigatus isolates carrying the G448S substitution in CYP51A gene after long-term treatment with voriconazole in an immunocompromised patient

Author:
Laís Pontes; Caio Augusto Gualtieri Beraquet; Teppei Arai; Akira Watanabe; Maria Luiza Moretti; Angelica Zaninelli Schreiber
Source:
Medical mycology case reports 2022 v.36 pp. 5-9
ISSN:
2211-7539
Subject:
amino acid substitution,   etc   ; Aspergillus fumigatus; antifungal agents; aspergillosis; azoles; genes; heart transplant; kidneys; mycology; patients;     Show all 10 Subjects
Abstract:
... We present a case of a 55-year-old man with a heart transplant who acquired Invasive Aspergillosis by Aspergillus fumigatus with the focus in the kidney. During about two years of antifungal treatment, most of the time with voriconazole, it was possible to obtain nine isolates of A. fumigatus, with the same genotypic characteristics, but with an increase in MIC for several azoles. The two last iso ...
DOI:
10.1016/j.mmcr.2022.02.002

4. Direct inhibition of the first PDZ domain of ZO-1 by glycyrrhizin is a possible mechanism of tight junction opening of Caco-2 cells

Author:
Emi Hibino; Natsuko Goda; Misaki Hisada; Takeshi Tenno; Hidekazu Hiroaki
Source:
Food & function 2022 v.13 no.4 pp. 1953-1964
ISSN:
2042-650X
Subject:
amino acid substitution,   etc   ; actin; deoxycholic acid; electrical resistance; glycyrrhetinic acid; glycyrrhizin; intestines; ligands; models; moieties; mutants; pharmacokinetics; tight junctions;     Show all 13 Subjects
Abstract:
... Glycyrrhizin (GL) is known to exhibit a variety of useful pharmacological activities, including anti-inflammation, anti-hepatotoxicity, and enhancement of intestinal drug absorption. GL has been reported to modify the assembly of actin filaments, thereby modulating tight junction (TJ) integrity, but the detailed molecular mechanisms of this remain unclear. In this study, we first found that GL bin ...
DOI:
10.1039/d1fo03062k

5. Opposite motion of the Central Helices of efflux pump KmrA is important for its export efficiency

Author:
Ying Li; Honglin Wen; Xizhen Ge
Source:
Microbial pathogenesis 2022 v.167 pp. 105570
ISSN:
0882-4010
Subject:
amino acid substitution,   etc   ; Klebsiella pneumoniae; antibiotic resistance; cross infection; ethidium; hydrogen bonding; hydrophilicity; hydrophobicity; models; molecular biology; molecular dynamics; pathogenesis; pathogens; transporters;     Show all 14 Subjects
Abstract:
... Efflux pump of Major Facilitator Superfamily (MFS) is widely distributed in bacteria, while its role in regulating antibiotic resistance of nosocomial pathogen Klebsiella pneumoniae remains unclear. Herein we analyzed the effect of amino acid substitution of MFS efflux pump KmrA on its export efficiency via molecular biology and molecular dynamics (MD). After searching across the 804 sequenced K. ...
DOI:
10.1016/j.micpath.2022.105570

6. Substandard starch grain4 may function in amyloplast development by influencing starch and lipid metabolism in rice endosperm

Author:
Yue Cai; Haiyuan Chen; Ning Xiao; Yunyu Wu; Ling Yu; Zichun Chen; Jianju Liu; Wei Shi; Cunhong Pan; Yuhong Li; Changhai Zhou; Hongjuan Ji; Niansheng Huang; Xiaoxiang Zhang; Yunhui Zhang; Aihong Li
Source:
Journal of plant physiology 2022 v.270 pp. 153638
ISSN:
0176-1617
Subject:
amino acid substitution,   etc   ; amyloplasts; amylose; aspartic acid; biosynthesis; biotechnology; endosperm; fatty acids; genetic improvement; grain quality; lipid metabolism; mutants; plant physiology; rice;     Show all 14 Subjects
Abstract:
... The amyloplast is a specialized plastid in rice endosperm cells where starch is synthesized and stored as starch granules (SGs). However, little is known about the molecular mechanism underlying amyloplast and SG development. In this study, a novel mutant (c134) demonstrating a floury endosperm with enlarged SGs and amyloplasts was identified. The floury endosperm was caused by rounder, loosely pa ...
DOI:
10.1016/j.jplph.2022.153638

7. Insight towards the effect of the multi basic cleavage site of SARS-CoV-2 spike protein on cellular proteases

Author:
Kamal Shokeen; Shambhavi Pandey; Manisha Shah; Sachin Kumar
Source:
Virus research 2022 v.318 pp. 198845
ISSN:
0168-1702
Subject:
amino acid substitution,   etc   ; Severe acute respiratory syndrome coronavirus 2; amino acids; cathepsin B; host range; molecular dynamics; pathogenesis; plasmin; proteolysis; research; tissue tropism; viruses;     Show all 12 Subjects
Abstract:
... Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection presents an immense global health problem. Spike (S) protein of coronavirus is the primary determinant of its entry into the host as it consists of both receptor binding and fusion domain. Besides tissue tropism, and host range, coronavirus pathogenesis are primarily controlled by the interaction of S protein with the cell rece ...
DOI:
10.1016/j.virusres.2022.198845

8. Circulation of SARS-CoV-2 Variants among Children from November 2020 to January 2022 in Trieste (Italy)

Author:
Maria Dolci; Lucia Signorini; Carolina Cason; Giuseppina Campisciano; Paolo Kunderfranco; Elena Pariani; Cristina Galli; Vincenzo Petix; Pasquale Ferrante; Serena Delbue; Manola Comar
Source:
Microorganisms 2022 v.10 no.3 pp. -
ISSN:
2076-2607
Subject:
amino acid substitution,   etc   ; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; amino acids; blood serum; children; genes; neutralization; patients; retrospective studies; viral load; Italy;     Show all 12 Subjects
Abstract:
... Introduction: The ongoing coronavirus disease 19 (COVID-19) outbreak involves the pediatric population, but to date, few reports have investigated the circulation of variants among children. Material and Methods: In this retrospective study, non-hospitalized pediatric patients with SARS-CoV-2-positive nasopharyngeal swabs (NPS) were enrolled at the Institute for Maternal and Child Health-IRCCS Bur ...
DOI:
10.3390/microorganisms10030612

9. A Single Amino Acid Substitution in RFC4 Leads to Endoduplication and Compromised Resistance to DNA Damage in Arabidopsis thaliana

Author:
Kan Cui; Lei Qin; Xianyu Tang; Jieying Nong; Jin Chen; Nan Wu; Xin Gong; Lixiong Yi; Chenghuizi Yang; Shitou Xia
Source:
Genes 2022 v.13 no.6 pp. -
ISSN:
2073-4425
Subject:
amino acid substitution,   etc   ; Arabidopsis thaliana; DNA; DNA damage; DNA repair; RNA; adenosinetriphosphatase; cell cycle; cell proliferation; dwarfing; genomics; genotoxicity; homozygosity; mutagens; mutants; phenotype; site-directed mutagenesis;     Show all 17 Subjects
Abstract:
... Replication factor C (RFC) is a heteropentameric ATPase associated with the diverse cellular activities (AAA+ATPase) protein complex, which is composed of one large subunit, known as RFC1, and four small subunits, RFC2/3/4/5. Among them, RFC1 and RFC3 were previously reported to mediate genomic stability and resistance to pathogens in Arabidopsis. Here, we generated a viable rfc4e (rfc4−1/RFC4ᴳ⁵⁴ᴱ ...
DOI:
10.3390/genes13061037

10. Reviewing Challenges of Predicting Protein Melting Temperature Change Upon Mutation Through the Full Analysis of a Highly Detailed Dataset with High-Resolution Structures

Author:
Benjamin B. V. Louis; Luciano A. Abriata
Source:
Molecular biotechnology 2021 v.63 no.10 pp. 863-884
ISSN:
1073-6085
Subject:
amino acid substitution,   etc   ; biotechnology; data collection; prediction; temperature;     Show all 5 Subjects
Abstract:
... Predicting the effects of mutations on protein stability is a key problem in fundamental and applied biology, still unsolved even for the relatively simple case of small, soluble, globular, monomeric, two-state-folder proteins. Many articles discuss the limitations of prediction methods and of the datasets used to train them, which result in low reliability for actual applications despite globally ...
DOI:
10.1007/s12033-021-00349-0

11. Mechanism of Fusarium graminearum Resistance to Ergosterol Biosynthesis Inhibitors: G443S Substitution of the Drug Target FgCYP51A

Author:
Huahua Zhao; Xian Tao; Wen Song; Haorong Xu; Meixia Li; Yiqiang Cai; Jianxin Wang; Yabing Duan; Mingguo Zhou
Source:
Journal of agricultural and food chemistry 2022 v.70 no.6 pp. 1788-1798
ISSN:
1520-5118
Subject:
amino acid substitution,   etc   ; Fusarium graminearum; Fusarium head blight; biosynthesis; drugs; ergosterol; fluorescence; food chemistry; fungi; fungicide resistance; genetic transformation; pathogens; risk; sexual reproduction; site-directed mutagenesis; sporulation; tebuconazole; virulence;     Show all 18 Subjects
Abstract:
... Fusarium head blight (FHB), caused by the Fusarium graminearum species complex, is a devastating fungal disease resulting in substantial yield and quality losses. Ergosterol biosynthesis inhibitors (EBIs) are the most popular chemicals for controlling FHB. Recently, the resistance of F. graminearum to EBIs has emerged in the field, and an amino acid substitution (G443S) of the sterol 14α-demethyla ...
DOI:
10.1021/acs.jafc.1c07543

12. The MSI1 member OsRBAP1 gene, identified by a modified MutMap method, is required for rice height and spikelet fertility

Author:
Xiaozhen Huang; Xiaofang Zeng; Mingling Cai; Degang Zhao
Source:
Plant science 2022 v.320 pp. 111201
ISSN:
0168-9452
Subject:
amino acid substitution,   etc   ; CRISPR-Cas systems; biogenesis; cell walls; cellulose; dwarfing; ethane; exons; glutamic acid; landraces; male sterility; mutants; nonsense mutation; plant height; progeny; rice; spikelets; sulfonates; transcriptome; transcriptomics; China;     Show all 21 Subjects
Abstract:
... To explore the molecular mechanisms underlying plant height regulation, we isolated and characterized a stably inherited semi-dwarf mutant bgsd-2 from the ethane methyl sulfonate (EMS) mutant progeny of ‘Ping Tang Wild-type (PTWT)’, a rice (Oryza sativa ssp. japonica) landrace in Guizhou. Transcriptome sequencing and qRT-PCR analyses showed that a number of cellulose and lignin-related genes invol ...
DOI:
10.1016/j.plantsci.2022.111201

13. Target site resistance to acetolactate synthase inhibitors in a fall panicum (Panicum dichotomiflorum Michx.) accession from Wisconsin and its response to alternative herbicides

Author:
Jose J. Nunes; Damilola A. Raiyemo; Nicholas J. Arneson; Alexandre T. Rosa; Patrick J. Tranel; Rodrigo Werle
Source:
Weed technology 2022 v.36 no.1 pp. 48-55
ISSN:
1550-2740
Subject:
amino acid substitution,   etc   ; 4-hydroxyphenylpyruvate dioxygenase; Panicum dichotomiflorum; Zea mays; acetolactate synthase; biomass; clethodim; dose response; genes; glufosinate; glyphosate; grasses; greenhouses; herbicide resistance; isoxaflutole; mesotrione; nicosulfuron; quizalofop; sweetcorn; tembotrione; weed control; weeds; Wisconsin;     Show all 23 Subjects
Abstract:
... Owing to the lack of effective POST herbicide options, producers typically rely on nicosulfuron as the main POST grass herbicide in sweet corn production systems. In 2019, a Wisconsin sweet corn producer reported fall panicum control escapes after spraying nicosulfuron. Seeds from mature plants were collected to (1) measure fall panicum response to acetolactate synthase (ALS)-inhibiting herbicides ...
DOI:
10.1017/wet.2021.104

14. A single amino acid substitution in the R2R3 conserved domain of the BrPAP1a transcription factor impairs anthocyanin production in turnip (Brassica rapa subsp. rapa)

Author:
Jianfei Yang; Hyon Dok Song; Yunzhu Chen; Bowei Chen; Minjun Kim; Pyol Kim; Saneyuki Kawabata; Yuhua Li; Yu Wang
Source:
Plant physiology and biochemistry 2021 v.162 pp. 124-136
ISSN:
0981-9428
Subject:
amino acid substitution,   etc   ; anthocyanins; biosynthesis; genes; mutants; pigmentation; plant physiology; serine; testa; transcription factors; turnips;     Show all 11 Subjects
Abstract:
... The purple pigmentation in the epidermis of swollen roots of ‘Tsuda’ turnip (Brassica rapa subsp. rapa) is induced by light, providing a good system to investigate the genetic mechanism of light-dependent anthocyanin biosynthesis in B. rapa. Here, we identified the R2R3 MYB transcription factor gene PRODUCTION OF ANTHOCYANIN PIGMENT1 (BrPAP1a) as the critical gene in the anthocyanin-defective muta ...
DOI:
10.1016/j.plaphy.2021.02.011

15. Genetic Characterizations and Molecular Evolution of the Measles Virus Genotype B3’s Hemagglutinin (H) Gene in the Elimination Era

Author:
Nan Zhou; Mingma Li; Yue Huang; Lu Zhou; Bei Wang
Source:
Viruses 2021 v.13 no.10 pp. -
ISSN:
1999-4915
Subject:
amino acid substitution,   etc   ; Measles morbillivirus; effective population size; genes; hemagglutinins; molecular epidemiology; selection pressure; subgenotype;     Show all 8 Subjects
Abstract:
... Measles virus (MeV) genotype B3 is one globally significant circulating genotype. Here, we present a systematic description of long-term evolutionary characterizations of the MeV genotype B3’s hemagglutinin (H) gene in the elimination era. Our results show that the B3 H gene can be divided into two main sub-genotypes, and the highest intra-genotypic diversity was observed in 2004. MeV genotype B3’ ...
DOI:
10.3390/v13101970

16. Identification of novel protein-coding sequences in Eucalyptus grandis plants by high-resolution mass spectrometry

Author:
Gabriel Lemes Jorge; Tiago Santana Balbuena
Source:
Biochimica et biophysica acta 2021 v.1869 no.3 pp. 140594
ISSN:
1570-9639
Subject:
amino acid substitution,   etc   ; Eucalyptus grandis; databases; forest industries; genome; genomics; mass spectrometry; peptides; proteomics; transcriptomics;     Show all 10 Subjects
Abstract:
... Eucalyptus species are widely used in the forestry industry, and a significant increase in the number of sequences available in database repositories has been observed for these species. In proteomics, a protein is identified by correlating the theoretical fragmentation spectrum derived from genomic/transcriptomic data against the experimental fragmentation mass spectrum acquired from large-scale ...
DOI:
10.1016/j.bbapap.2020.140594

17. A Novel Pinkish-White Flower Color Variant Is Caused by a New Allele of Flower Color Gene W1 in Wild Soybean (Glycine soja)

Author:
Jagadeesh Sundaramoorthy; Gyu-Tae Park; Hyun Jo; Jeong-Dong Lee; Hak-Soo Seo; Jong-Tae Song
Source:
Agronomy 2021 v.11 no.5 pp. -
ISSN:
2073-4395
Subject:
amino acid substitution,   etc   ; Glycine soja; agronomy; alleles; anthocyanins; color; enzymes; flower color; flowers; loci; mutants; soybeans;     Show all 12 Subjects
Abstract:
... The enzyme flavonoid 3′,5′-hydroxylase (F3′5′H) plays an important role in producing anthocyanin pigments in soybean. Loss of function of the W1 locus encoding F3′5′H always produces white flowers. However, few color variations have been reported in wild soybean. In the present study, we isolated a new color variant of wild soybean accession (IT261811) with pinkish-white flowers. We found that the ...
DOI:
10.3390/agronomy11051001

18. HvLEAFY controls the early stages of floral organ specification and inhibits the formation of multiple ovaries in barley

Author:
Caterina Selva; Neil J. Shirley; Kelly Houston; Ryan Whitford; Ute Baumann; Gang Li; Matthew R. Tucker
Source:
plant journal 2021 v.108 no.2 pp. 509-527
ISSN:
0960-7412
Subject:
amino acid substitution,   etc   ; Arabidopsis; Hordeum vulgare; alleles; barley; chromosomes; flowering; flowers; mutants; phenotype; proline; reproductive success; rice;     Show all 13 Subjects
Abstract:
... Transition to the reproductive phase, inflorescence formation and flower development are crucial elements that ensure maximum reproductive success in a plant’s life cycle. To understand the regulatory mechanisms underlying correct flower development in barley (Hordeum vulgare), we characterized the multiovary 5 (mov5.o) mutant. This mutant develops abnormal flowers that exhibit mosaic floral organ ...
DOI:
10.1111/tpj.15457

19. Characterization of a variant CD4 molecule in Japanese Black cattle

Author:
Yuko Kato-Mori; Taku Okamura; Norihito Kawashita; Katsuro Hagiwara
Source:
Veterinary immunology and immunopathology 2021 v.232 pp. 110167
ISSN:
0165-2427
Subject:
amino acid substitution,   etc   ; Wagyu; amino acids; cytokines; epitopes; farms; flow cytometry; gene expression; genes; glycoproteins; immunopathology; macrophages; mitogens; monocytes;     Show all 14 Subjects
Abstract:
... Monoclonal antibodies (mAbs) that recognize cluster of differentiation (CD) molecules on lymphocytes are useful tools for the study of different lymphocyte subsets in flow cytometry (FCM) analysis. CD4 is a glycoprotein found on the surfaces of helper T cells, monocytes, macrophages, and dendritic cells. In this study, we describe Japanese Black (JB) calves in a farm whose peripheral blood mononuc ...
DOI:
10.1016/j.vetimm.2020.110167

20. Single nucleotide polymorphism in avian uncoupling protein gene is associated with thermoregulation in chicks

Author:
Yoshimitsu Ouchi; Vishwajit S. Chowdhury; John F. Cockrem; Takashi Bungo
Source:
Animal science journal = 2021 v.92 no.1 pp. e13632
ISSN:
1344-3941
Subject:
amino acid substitution,   etc   ; ambient temperature; body temperature; exons; heat production; heat transfer; mutants; oxygen consumption; single nucleotide polymorphism; thermoregulation;     Show all 10 Subjects
Abstract:
... Avian uncoupling protein (av‐UCP) is a key protein for thermoregulation in poultry. A single nucleotide polymorphism (SNP) in the av‐UCP gene has been reported in chickens. The purpose of the current study was to clarify the association between this av‐UCP gene mutation and thermoregulation in chickens. Wild and mutant type chicks for the av‐UCP gene SNP (g. 1270 of the av‐UCP gene exon 3 with C t ...
DOI:
10.1111/asj.13632