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1. Aztreonam-avibactam may not replace ceftazidime/avibactam: the case of KPC-21 carbapenemase and penicillin-binding protein 3 with four extra amino acids

Author:
Ke Ma; Yu Feng; Zhiyong Zong
Source:
International journal of antimicrobial agents 2022 v.60 no.3 pp. 106642
ISSN:
0924-8579
Subject:
amino acid substitution,   etc   ; Escherichia coli; beta-lactamase; mutants;     Show all 4 Subjects
Abstract:
... Aztreonam/avibactam is a promising antimicrobial combination with additional coverage for metallo-β-lactamases compared with ceftazidime/avibactam. A carbapenem-resistant blaKPC₋₂-carrying Escherichia coli clinical isolate had four extra amino acids in penicillin-binding protein 3 (PBP3), which has been known to mediate reduced susceptibility to aztreonam/avibactam. This prompted us to investigate ...
DOI:
10.1016/j.ijantimicag.2022.106642

2. Engineering and screening of novel β-1,3-xylanases with desired hydrolysate type by optimized ancestor sequence reconstruction and data mining

Author:
Bo Zeng; ShuYan Zhao; Rui Zhou; YanHong Zhou; WenHui Jin; ZhiWei Yi; GuangYa Zhang
Source:
Computational and Structural Biotechnology Journal 2022 v.20 pp. 3313-3321
ISSN:
2001-0370
Subject:
amino acid substitution,   etc   ; ancestry; biotechnology; hydrolases; hydrolysates; mutagenesis; oligosaccharides;     Show all 7 Subjects
Abstract:
... Engineering of hydrolases to shift their hydrolysate types has not been attempted so far, though computer-assisted enzyme design has been successful. A novel integrative strategy for engineering and screening the β-1,3-xylanase with desired hydrolysate types was proposed, with the purpose to solve problems that the separation and preparation of β-1,3-xylo-oligosaccharides was in high cost yet in l ...
DOI:
10.1016/j.csbj.2022.06.050

3. Global epidemiology of CTX-M-type β-lactam resistance in human and animal

Author:
Chanchal Rana; Shiveeli Rajput; Manisha Behera; Devika Gautam; Vaibhav Vikas; Ashutosh Vats; Mayank Roshan; Soma M. Ghorai; Sachinandan De
Source:
Comparative immunology, microbiology, and infectious diseases 2022 v.86 pp. 101815
ISSN:
0147-9571
Subject:
amino acid substitution,   etc   ; Japan; epidemiology; humans; immunology; Europe; India;     Show all 7 Subjects
Abstract:
... CTX-M ESBL are widely found in animal and human infections. For better understanding of CTX-M variations and epidemiology, a total of 2210 CTX-M sequences were retrieved from NCBI as at 20 December 2020. The maximum incidences of CTX-M were reported in China (n = 508), USA (n = 354) and Japan (n = 180). Single amino acid substitution in the domain region of CTX-M ESBL lead to survival benefits to ...
DOI:
10.1016/j.cimid.2022.101815

4. Single-Nucleotide Polymorphisms in Bmy1 Intron III Alleles Conferring the Genotypic Variations in β-Amylase Activity under Drought Stress between Tibetan Wild and Cultivated Barley

Author:
Xiaojian Wu; Wenhao Yue; Kangfeng Cai; Huan Wang; Fanrong Zeng; Junmei Wang
Source:
Agronomy 2022 v.12 no.8 pp. -
ISSN:
2073-4395
Subject:
amino acid substitution,   etc   ; agronomy; alleles; amino acid composition; barley; cultivars; drought; introns; water stress;     Show all 9 Subjects
Abstract:
... β-amylase activity is related to the polymorphisms of Bmy1 intron III; however, no attention has been given to such relationships under environmental stresses such as drought. In this study, 73 cultivated barley genotypes and 52 Tibetan wild barley accessions were used to test the association between Bmy1 gene intron III polymorphisms and β-amylase activity under drought stress. Our results showed ...
DOI:
10.3390/agronomy12081737

5. The base and root of domain II loops of Cry toxins contribute to binding to Bombyx mori ABC transporter C2

Author:
Satomi Adegawa; Naomi Yamaguchi; Ryoichi Sato
Source:
FEBS journal 2022 v.289 no.4 pp. 965-984
ISSN:
1742-464X
Subject:
amino acid substitution,   etc   ; ABC transporters; Bombyx mori; crosslinking; cysteine; mutants; surface plasmon resonance; toxins;     Show all 8 Subjects
Abstract:
... Little information is available regarding the region of Cry toxins involved in binding to their major receptors, the ATP‐binding cassette (ABC) transporters. We analyzed which Cry1Aa amino acid residues contribute to binding to Bombyx mori ABC transporter C2 (BmABCC2). Several two oxidized double‐cysteine substitution mutant toxins were made. In these, two amino acids at distant positions on toxin ...
DOI:
10.1111/febs.16224

6. Selection of Aspergillus fumigatus isolates carrying the G448S substitution in CYP51A gene after long-term treatment with voriconazole in an immunocompromised patient

Author:
Laís Pontes; Caio Augusto Gualtieri Beraquet; Teppei Arai; Akira Watanabe; Maria Luiza Moretti; Angelica Zaninelli Schreiber
Source:
Medical mycology case reports 2022 v.36 pp. 5-9
ISSN:
2211-7539
Subject:
amino acid substitution,   etc   ; Aspergillus fumigatus; antifungal agents; aspergillosis; azoles; genes; heart transplant; kidneys; mycology; patients;     Show all 10 Subjects
Abstract:
... We present a case of a 55-year-old man with a heart transplant who acquired Invasive Aspergillosis by Aspergillus fumigatus with the focus in the kidney. During about two years of antifungal treatment, most of the time with voriconazole, it was possible to obtain nine isolates of A. fumigatus, with the same genotypic characteristics, but with an increase in MIC for several azoles. The two last iso ...
DOI:
10.1016/j.mmcr.2022.02.002

7. A previously unappreciated polymorphism in the beta chain of I-As expressed in autoimmunity-prone SJL mice: Combined impact on antibody, CD4 T cell recognition and MHC class II dimer structural stability

Author:
Katherine A. Richards; Courtney Lavery; Grant L.J. Keller; Jim Miller; Brian M. Baker; Andrea J. Sant
Source:
Molecular immunology 2022 v.143 pp. 17-26
ISSN:
0161-5890
Subject:
amino acid substitution,   etc   ; CD4-positive T-lymphocytes; autoimmunity; hydrogen; ligands; molecular dynamics; monoclonal antibodies; peptides;     Show all 8 Subjects
Abstract:
... In the process of structure-function studies on the MHC class II molecule expressed in autoimmunity prone SJL mice, I-Aˢ, we discovered a disparity from the reported sequence of the MHC class II beta chain. The variant is localized at a highly conserved site of the beta chain, at residue 58. Our studies revealed that this single amino acid substitution of Pro for Ala at this residue, found in I-Aˢ ...
DOI:
10.1016/j.molimm.2021.12.022

8. A single amino acid substitution alters activity and specificity in Plasmodium falciparum aspartyl & asparaginyl-tRNA synthetases

Author:
Vivek Kumar Sharma; Swati Gupta; Jyoti Chhibber-Goel; Manickam Yogavel; Amit Sharma
Source:
Molecular and biochemical parasitology 2022 v.250 pp. 111488
ISSN:
0166-6851
Subject:
amino acid substitution,   etc   ; Plasmodium falciparum; amino acids; aminoacyl tRNA ligases; aminoacylation; enzyme activity; esterification; parasitology;     Show all 8 Subjects
Abstract:
... The specificity of each aminoacyl-tRNA synthetase (aaRS) for its cognate amino acid ensures correct tRNA esterification and allows fidelity in protein synthesis. The aaRSs discriminate based on the chemical properties of their amino acid substrates and structural features of the binding pockets. In this study, we characterized aspartyl-(DRS) and asparaginyl-tRNA synthetase (NRS) from Plasmodium fa ...
DOI:
10.1016/j.molbiopara.2022.111488

9. Effect of a single amino acid substitution G98D in a ribosome-associated essential GTPase, CgtA, on the growth and morphology of Vibrio cholerae

Author:
Sagarika Das; Partha Pratim Datta
Source:
Archives of microbiology 2022 v.204 no.10 pp. 617
ISSN:
0302-8933
Subject:
amino acid substitution,   etc   ; Vibrio cholerae; guanosinetriphosphatase; microbiology; mutants; statistical analysis; stress response; transmission electron microscopy;     Show all 8 Subjects
Abstract:
... CgtA, a highly conserved 50S ribosome-associated essential GTPase, acts as a repressor of the stringent stress response under nutrient-rich growth conditions to suppress basal levels of the alarmone ppGpp in V. cholerae. To further explore the in vivo functionality of CgtA, we introduced an amino acid substitution, i.e., Gly98Asp, in a conserved glycine residue in the N-terminal domain. The constr ...
DOI:
10.1007/s00203-022-03233-w

10. The G143A/S substitution of mitochondrially encoded cytochrome b (Cytb) in Magnaporthe oryzae confers resistance to quinone outside inhibitors

Author:
Tao Li; Jinke Xu; Han Gao; Zhiguo Cao; Jianxin Wang; Yiqiang Cai; Yabing Duan; Mingguo Zhou
Source:
Pest management science 2022 v.78 no.11 pp. 4850-4858
ISSN:
1526-498X
Subject:
amino acid substitution,   etc   ; Magnaporthe oryzae; blast disease; cross resistance; cytochrome b; normal distribution; picoxystrobin; quinones; risk;     Show all 9 Subjects
Abstract:
... BACKGROUND: Rice blast, caused by Magnaporthe oryzae, is a destructive disease threatening the production of staple foods worldwide. Quinone outside inhibitors (QoIs) are a group of chemicals exhibiting excellent activity against a majority of plant pathogens, with the disadvantage that pathogens can easily develop resistance to QoIs. RESULTS: Here, we investigated the activity of picoxystrobin ag ...
DOI:
10.1002/ps.7106

11. Evolutionary Conservation of PP2A Antagonism and G2/M Cell Cycle Arrest in Maedi-Visna Virus Vif

Author:
Adeline M. Luperchio; Stefán R. Jónsson; Daniel J. Salamango
Source:
Viruses 2022 v.14 no.8 pp. -
ISSN:
1999-4915
Subject:
amino acid substitution,   etc   ; Simian immunodeficiency virus; Visna maedi virus; antagonism; cell cycle checkpoints; cyclophilins; mutagens; pathogenesis; phenotype; phosphoproteome;     Show all 10 Subjects
Abstract:
... The canonical function of lentiviral Vif proteins is to counteract the mutagenic potential of APOBEC3 antiviral restriction factors. However, recent studies have discovered that Vif proteins from diverse HIV-1 and simian immunodeficiency virus (SIV) isolates degrade cellular B56 phosphoregulators to remodel the host phosphoproteome and induce G2/M cell cycle arrest. Here, we evaluate the conservat ...
DOI:
10.3390/v14081701

12. A novel NPHS2 mutation (c.865A > G) identified in a Chinese family with steroid-resistant nephrotic syndrome alters subcellular localization of nephrin

Author:
Na Wu; Yingchuan Zhu; Wenhao Jiang; Yue Song; Lan Yin; Yilu Lu; Dachang Tao; Yunqiang Liu; Yongxin Ma
Source:
Genes & genomics 2022 v.44 no.5 pp. 551-559
ISSN:
1976-9571
Subject:
amino acid substitution,   etc   ; diaphragm; fluorescent antibody technique; genes; genomics; heterozygosity; immunoblotting; mutants; nephrotic syndrome; plasma membrane; precipitin tests;     Show all 11 Subjects
Abstract:
... BACKGROUND: NPHS2 is the causative gene of nephrotic syndrome type 2 (MIM 600995) which often clinically manifests as steroid-resistant nephrotic syndrome (SRNS). The NPHS2 gene encodes a slit diaphragm (SD) associated protein podocin. OBJECTIVE: This study reported a novel disease-causing mutation of NPHS2 in a Chinese family with SRNS. We also investigated the pathogenic mechanism of the variant ...
DOI:
10.1007/s13258-022-01220-5

13. Direct inhibition of the first PDZ domain of ZO-1 by glycyrrhizin is a possible mechanism of tight junction opening of Caco-2 cells

Author:
Emi Hibino; Natsuko Goda; Misaki Hisada; Takeshi Tenno; Hidekazu Hiroaki
Source:
Food & function 2022 v.13 no.4 pp. 1953-1964
ISSN:
2042-650X
Subject:
amino acid substitution,   etc   ; actin; deoxycholic acid; electrical resistance; glycyrrhetinic acid; glycyrrhizin; intestines; ligands; models; moieties; mutants; pharmacokinetics; tight junctions;     Show all 13 Subjects
Abstract:
... Glycyrrhizin (GL) is known to exhibit a variety of useful pharmacological activities, including anti-inflammation, anti-hepatotoxicity, and enhancement of intestinal drug absorption. GL has been reported to modify the assembly of actin filaments, thereby modulating tight junction (TJ) integrity, but the detailed molecular mechanisms of this remain unclear. In this study, we first found that GL bin ...
DOI:
10.1039/d1fo03062k

14. Two independently evolved natural mutations additively deregulate TyrA enzymes and boost tyrosine production in planta

Author:
Samuel Lopez‐Nieves; Jorge El‐Azaz; Yusen Men; Cynthia K. Holland; Tao Feng; Samuel F. Brockington; Joseph M. Jez; Hiroshi A. Maeda
Source:
plant journal 2022 v.109 no.4 pp. 844-855
ISSN:
0960-7412
Subject:
amino acid substitution,   etc   ; Arabidopsis; Caryophyllales; Fabaceae; Nicotiana benthamiana; active sites; betalains; essential amino acids; legumes; oxidoreductases; protein synthesis; tyrosine;     Show all 12 Subjects
Abstract:
... l‐Tyrosine is an essential amino acid for protein synthesis and is also used in plants to synthesize diverse natural products. Plants primarily synthesize tyrosine via TyrA arogenate dehydrogenase (TyrAₐ or ADH), which are typically strongly feedback inhibited by tyrosine. However, two plant lineages, Fabaceae (legumes) and Caryophyllales, have TyrA enzymes that exhibit relaxed sensitivity to tyro ...
DOI:
10.1111/tpj.15597

15. GAREM1 is involved in controlling body mass in mice and humans

Author:
Tasuku Nishino; Takaya Abe; Mari Kaneko; Masanao Yokohira; Keiko Yamakawa; Katsumi Imaida; Hiroaki Konishi
Source:
Biochemical and biophysical research communications 2022 v.628 pp. 91-97
ISSN:
0006-291X
Subject:
amino acid substitution,   etc   ; body size; body weight; brain; cell growth; cell proliferation; genome; growth retardation; mutants; patients; phosphorylation; research;     Show all 12 Subjects
Abstract:
... The adaptor protein GAREM has two subtypes. Each is involved in Erk activation signaling downstream of the cell growth factor receptor in cultured cells. Regarding their role in individual animals, we have previously reported that mice deficient in GAREM2, which is highly expressed in the brain, exhibit emotional changes. In this paper, we report an amino acid substitution mutation (K291R) in GARE ...
DOI:
10.1016/j.bbrc.2022.08.058

16. Compound heterozygous mutations of NDUFV1 identified in a child with mitochondrial complex I deficiency

Author:
Xiaojun Tang; Wuhen Xu; Xiaozhen Song; Haiyun Ye; Xiang Ren; Yongchen Yang; Hong Zhang; Shengnan Wu; Xiaoping Lan
Source:
Genes & genomics 2022 v.44 no.6 pp. 691-698
ISSN:
1976-9571
Subject:
amino acid substitution,   etc   ; cardiomyopathy; childhood; children; computer simulation; databases; encephalopathy; genomics; heterozygosity; missense mutation; mitochondria; muscular diseases; patients; prediction; reproduction;     Show all 15 Subjects
Abstract:
... BACKGROUND: Mitochondrial complex I deficiency (MCID) is the most common biochemical defect identified in childhood with mitochondrial diseases, mainly including Leigh syndrome, encephalopathy, macrocephaly with progressive leukodystrophy, hypertrophic cardiomyopathy and myopathy. OBJECTIVE: To identify genetic cause in a patient with early onset autosomal recessive MCID. METHODS: Trio whole-exome ...
DOI:
10.1007/s13258-022-01260-x

17. Truncated analog Brevinin2-CE-N26V5K: Revelation the Augmentation of Antimicrobial Activity

Author:
Yi Zhao; Xiao-yan Wang; Yan Sun; Zhi Li; Tao Liu; Qing-mei Liu; Jingyi Chen
Source:
World journal of microbiology & biotechnology 2022 v.38 no.9 pp. 162
ISSN:
0959-3993
Subject:
amino acid substitution,   etc   ; Rana chensinensis; antibacterial properties; atomic force microscopy; biotechnology; cell membranes; forests; frogs; hemolysis; hydrophilicity; hydrophobicity; peptides; surfactants; viability;     Show all 14 Subjects
Abstract:
... Brevinin2-CE (B2CE), a natural peptide containing 37 amino acids, was first isolated from the skin secretions of the Chinese forest frog Rana chensinensis. B2CE shows good antibacterial activity. In this study, a series of B2CE analogs with differences in cationicity, α-helicity, hydrophobicity and amphipathic properties were designed through chain-length deletion and amino acid substitution. The ...
DOI:
10.1007/s11274-022-03333-1

18. Genome‐informed characterisation of antigenic drift in the haemagglutinin gene of equine influenza strains circulating in the United States from 2012 to 2017

Author:
Kyuyoung Lee; Nicola Pusterla; Samantha M. Barnum; Dong‐Hun Lee; Beatriz Martínez‐López
Source:
Transboundary and emerging diseases 2022 v.69 no.4 pp. e52
ISSN:
1865-1674
Subject:
amino acid substitution,   etc   ; Equidae; Influenza A virus; amino acids; antigenic variation; biosecurity; equine influenza; genes; glycosylation; hemagglutinins; horses; pathogens; phylogeny; vaccines;     Show all 14 Subjects
Abstract:
... Equine influenza virus (EIV) is a major infectious pathogen causing significant respiratory signs in equids worldwide. Voluntary surveillances in the United States recently reported EIV detection in horses with respiratory signs even with adequate vaccine protocols and biosecurity programs and posed a concern about suboptimal effectiveness of EIV vaccine in the United States. This study aims to de ...
DOI:
10.1111/tbed.14262

19. Opposite motion of the Central Helices of efflux pump KmrA is important for its export efficiency

Author:
Ying Li; Honglin Wen; Xizhen Ge
Source:
Microbial pathogenesis 2022 v.167 pp. 105570
ISSN:
0882-4010
Subject:
amino acid substitution,   etc   ; Klebsiella pneumoniae; antibiotic resistance; cross infection; ethidium; hydrogen bonding; hydrophilicity; hydrophobicity; models; molecular biology; molecular dynamics; pathogenesis; pathogens; transporters;     Show all 14 Subjects
Abstract:
... Efflux pump of Major Facilitator Superfamily (MFS) is widely distributed in bacteria, while its role in regulating antibiotic resistance of nosocomial pathogen Klebsiella pneumoniae remains unclear. Herein we analyzed the effect of amino acid substitution of MFS efflux pump KmrA on its export efficiency via molecular biology and molecular dynamics (MD). After searching across the 804 sequenced K. ...
DOI:
10.1016/j.micpath.2022.105570

20. Rapidly Increasing Boscalid Resistance in Corynespora cassiicola in China

Author:
Bingxue Sun; Guangxue Zhu; Xuewen Xie; Ali Chai; Lei Li; Tengfei Fan; Baoju Li; Shi Yanxia
Source:
Phytopathology 2022 v.112 no.8 pp. 1659-1666
ISSN:
0031-949X
Subject:
amino acid substitution,   etc   ; Corynespora cassiicola; benzimidazole; boscalid; cucumbers; foliar diseases; genotype; histidine; leaf spot; plant pathology; quinones; succinate dehydrogenase (quinone); tyrosine; China;     Show all 14 Subjects
Abstract:
... Corynespora leaf spot caused by Corynespora cassiicola is an important foliar disease in cucumber. Succinate dehydrogenase inhibitors are the main fungicides used to control this disease. With the application of succinate dehydrogenase inhibitors (SDHIs) in the field, boscalid-resistant isolates have been continuously detected in the field. Resistance monitoring programs were performed to investig ...
DOI:
10.1094/PHYTO-12-21-0503-R