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1. Facile Strategy for Visible Disassembly of Spherical Nucleic Acids Programmed by Catalytic DNA Circuits

Author:
Wei, Bing; Yao, Dongbao; Zheng, Bin; Zhou, Xiang; Guo, Yijun; Li, Xiang; Li, Chengxu; Xiao, Shiyan; Liang, Haojun
Source:
ACS applied materials & interfaces 2019 v.11 no.22 pp. 19724-19733
ISSN:
1944-8252
Subject:
analytical kits,   etc   ; DNA; colorimetry; nanoparticles; phase transition; single nucleotide polymorphism;     Show all 6 Subjects
Abstract:
... The programmable toehold-mediated DNA-strand-displacement reaction has demonstrated its extraordinary capability in driving the spherical nucleic acid assembly. Here, a facile strategy of integrating a DNA-strand-displacement-based DNA circuit with a universal spherical nucleic acid aggregate system was developed for the visible disassembly of spherical nucleic acids. This integrated system exhibi ...
DOI:
10.1021/acsami.9b02107

2. Rapid and equipment‐free detection of Phytophthora capsici using lateral flow strip‐based recombinase polymerase amplification assay

Author:
Yu, J.; Shen, D.; Dai, T.; Lu, X.; Xu, H.; Dou, D.
Source:
Letters in applied microbiology 2019 v.69 no.1 pp. 64-70
ISSN:
0266-8254
Subject:
analytical kits,   etc   ; DNA; Phytophthora capsici; crops; detection limit; disease control; fungi; pathogens; pepper; rapid methods;     Show all 10 Subjects
Abstract:
... Phytophthora capsici is an important oomycete pathogen that causes devastating diseases in various crops. Methods for the rapid detection of P. capsici are important for disease control and eradication programmes. Here, we developed an assay based on lateral flow strip‐based recombinase polymerase amplification (LF‐RPA) for the rapid and equipment‐free detection of P. capsici. The specific primers ...
DOI:
10.1111/lam.13166

3. Using immobilization as a quick diagnostic indicator for resistance to phosphine

Author:
Athanassiou, Christos G.; Kavallieratos, Nickolas G.; Brabec, Daniel L.; Agrafioti, Paraskevi; Sakka, Maria; Campbell, James F.
Source:
Journal of stored products research 2019 v.82 pp. 17-26
ISSN:
0022-474X
Subject:
analytical kits,   etc   ; adults; exposure duration; insects; phosphine; walking; Australia; Germany; Greece; Spain; United States;     Show all 11 Subjects
Abstract:
... In the present study, we evaluated a quick diagnostic test for resistance to phosphine in stored-product beetle species. We collected different populations of thirteen species, obtained from different laboratories in different counties, i.e., USA, Greece, Australia, Germany and Spain. There were also tested populations that have been sampled from different facilities (field populations). We used t ...
DOI:
10.1016/j.jspr.2019.01.004

4. The end user sensor tree: An end-user friendly sensor database

Author:
Nelis, J.L.D.; Tsagkaris, A.S.; Zhao, Y.; Lou-Franco, J.; Nolan, P.; Zhou, H.; Cao, C.; Rafferty, K.; Hajslova, J.; Elliott, C.T.; Campbell, K.
Source:
Biosensors & bioelectronics 2019 v.130 pp. 245-253
ISSN:
0956-5663
Subject:
analytical kits,   etc   ; biosensors; business enterprises; databases; decision support systems; electrochemistry; experts; markets; mycotoxins; nanomaterials; pesticides; trees;     Show all 12 Subjects
Abstract:
... Detailed knowledge regarding sensor based technologies for the detection of food contamination often remains concealed within scientific journals or divided between numerous commercial kits which prevents optimal connectivity between companies and end-users. To overcome this barrier The End user Sensor Tree (TEST) has been developed. TEST is a comprehensive, interactive platform including over 900 ...
DOI:
10.1016/j.bios.2019.01.055

5. Geological data and outreach methods for designing targeted home radon testing programs

Author:
Wu, Connor Y. H.; Bennett, Michael; Fox, Katherine; Hubbard, Aaron; Parkhurst, Laura; Sherrod, Rebecca; Holguin, Sarah; Gohlke, Julia M.; Marmagas, Susan W.
Source:
Environmental earth sciences 2019 v.78 no.4 pp. 120
ISSN:
1866-6280
Subject:
analytical kits,   etc   ; United States Geological Survey; dolomite; education; neoplasms; outreach; radon; risk reduction; Virginia; West Virginia;     Show all 10 Subjects
Abstract:
... Indoor radon exposure is the second leading cause of lung cancer in the United States, yet radon risk reduction programs often struggle to increase home radon testing rates. This study compared two outreach methods to improve home radon testing and examined the association between home radon levels and geologic data in Central Appalachian communities in Tazewell County, Virginia, and Mercer County ...
DOI:
10.1007/s12665-019-8123-5

6. Human DNA degradation assessment and male DNA detection by quantitative-PCR followed by high-resolution melting analysis

Author:
Ginart, S.; Caputo, M.; Corach, D.; Sala, A.
Source:
Forensic science international 2019 v.295 pp. 1-7
ISSN:
0379-0738
Subject:
analytical kits,   etc   ; DNA; cost effectiveness; crime; females; humans; males; melting; quantitative polymerase chain reaction;     Show all 9 Subjects
Abstract:
... We developed a q-PCR technique that simultaneously evaluates the extent of degradation and determines the gender of a human DNA donor. QYDEG HRM is a triplex real-time PCR whose products are analysed by high-resolution melting (HRM). The system produces three amplicons: (1) transducin (beta)-like 1, Y-linked (TBL1Y) (84bp); (2) large-target sequence (DGlt) (244bp); and (3) small-target sequence (D ...
DOI:
10.1016/j.forsciint.2018.11.013

7. An alternative DNA extraction method for detection of Blastocystis spp. in human fecal samples

Author:
Karasartova, Djursun; Gureser, Ayse Semra; Ruh, Emrah; Turegun- Atasoy, Buse; Calgin, Mustafa Kerem; Tasci, Leyla; Taylan-Ozkan, Aysegul
Source:
Experimental parasitology 2018 v.186 pp. 36-41
ISSN:
0014-4894
Subject:
analytical kits,   etc   ; Blastocystis; DNA; feces; humans; parasites; polymerase chain reaction;     Show all 7 Subjects
Abstract:
... Polymerase chain reaction (PCR) is an effective technique for diagnosis of Blastocystis infection. Notably, DNA isolation procedure is extremely critical for the PCR step. In the present study, a recently described extraction procedure, named as the “sand method” was modified and adapted for isolation of Blastocystis DNA. To evaluate its efficacy, the current method and QIAamp DNA Stool Mini Kit ( ...
DOI:
10.1016/j.exppara.2018.01.019

8. Updating the ELISA standard curve fitting process to reduce uncertainty in estimated microcystin concentrations

Author:
Nummer, Stephanie A.; Weeden, Alexandra J.; Shaw, Chloe; Snyder, Brenda K.; Bridgeman, Thomas B.; Qian, Song S.
Source:
MethodsX 2018 v.5 pp. 304-311
ISSN:
2215-0161
Subject:
analytical kits,   etc   ; enzyme-linked immunosorbent assay; microcystins; statistical models; uncertainty;     Show all 5 Subjects
Abstract:
... This study is aimed at exploring the optimal ELISA standard curve fitting process for reducing measurement uncertainty. Using an ELISA kit for measuring cyanobacterial toxin (microcystin), we show that uncertainty associated with the estimated microcystin concentrations can be reduced by defining the standard curve as a four-parameter logistic function on the natural log concentration scale, inste ...
DOI:
10.1016/j.mex.2018.03.011

9. Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables

Author:
Costa, Magdalena; Sucari, Adriana; Epszteyn, Sergio; Oteiza, Juan; Gentiluomo, Jimena; Melamed, Celia; Figueroa, Yamila; Mingorance, Santiago; Grisaro, Agustina; Spioussas, Silvia; Almeida, Mariana Buffoni; Caruso, Mailen; Pontoni, Andrés; Signorini, Marcelo; Leotta, Gerardo
Source:
Food microbiology 2019 v.84 pp. 103273
ISSN:
0740-0020
Subject:
analytical kits,   etc   ; Shiga toxin-producing Escherichia coli; chard; disease severity; disease transmission; genes; meat; microbiology; serotypes; Argentina;     Show all 10 Subjects
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) are important pathogens transmitted by food that may cause severe illness in human beings. Thus, systems for STEC detection in food should have increasingly higher sensitivity and specificity. Here we compared six commercial systems for non-O157 STEC detection in meat and vegetables and determined their sensitivity, specificity and repeatability. A tot ...
DOI:
10.1016/j.fm.2019.103273

10. Isolating DNA sourced non-invasively from koala scats: a comparison of four commercial DNA stool kits

Author:
Wedrowicz, Faye; Mosse, Jennifer; Wright, Wendy; Hogan, Fiona E.
Source:
Conservation genetics resources 2019 v.11 no.2 pp. 219-229
ISSN:
1877-7252
Subject:
analytical kits,   etc   ; DNA; Phascolarctos cinereus; capillary electrophoresis; data quality; feces; genetic analysis; genotype; genotyping; quantitative polymerase chain reaction; soil;     Show all 11 Subjects
Abstract:
... Genetic sampling from faeces is a useful method for obtaining DNA samples non-invasively. The quantity and quality of DNA isolated from faecal samples is, however, an important factor affecting the success of downstream analyses. Commercial DNA isolation kits offer an efficient and convenient means for recovering DNA, but the kit methodology can influence the quantity and quality of DNA obtained. ...
DOI:
10.1007/s12686-018-0994-3

11. Vincristine combination with Ca+2 channel blocker increase antitumor effects

Author:
Taghizadehghalehjoughi, Ali; Sezen, Selma; Hacimuftuoglu, Ahmet; Güllüce, Medine
Source:
Molecular biology reports 2019 v.46 no.2 pp. 2523-2528
ISSN:
0301-4851
Subject:
analytical kits,   etc   ; antineoplastic activity; calcium channel blockers; calcium channels; cortex; humans; neoplasm cells; neoplasms; neurons; toxicity testing; viability; vincristine;     Show all 12 Subjects
Abstract:
... In this study, it was aimed to determine the effects of Amlodipine, a calcium channel blocker and vincristine (VCR) an antineoplastic, on human neuroblastomas using different doses. The cytotoxicity assays of the study were performed using the MTT method depending on time and concentration. After obtaining the mixture (up to 85% for SH-SY5Y) and sufficient branches (cortex neurons), the cells were ...
DOI:
10.1007/s11033-019-04706-w

12. Revealing the Effects of Curcumin on SH-SY5Y Neuronal Cells: A Combined Study from Cellular Viability, Morphology, and Biomechanics

Author:
Yu, Xiaoting; Chen, Ligang; Tang, Mingjie; Yang, Zhongbo; Fu, Ailing; Wang, Zhanzhong; Wang, Huabin
Source:
Journal of agricultural and food chemistry 2019 v.67 no.15 pp. 4273-4279
ISSN:
1520-5118
Subject:
analytical kits,   etc   ; adhesion; biomechanics; cell viability; curcumin; dose response; drugs; elastic deformation; image analysis; neurons; synapse; viability assays;     Show all 12 Subjects
Abstract:
... In this work, the effects of curcumin on the viability, morphology, and nanomechanics of SH-SY5Y neuronal cells were investigated using a conventional cell viability test kit (CCK-8) and sophisticated AFM imaging and force measurement techniques. CCK-8 tests show that SH-SY5Y neuronal cells have a dose–response to curcumin in terms of viability that is dependent on the exposure durations. When exp ...
DOI:
10.1021/acs.jafc.9b00314

13. Duplex PCR-ELONA for the detection of pork adulteration in meat products

Author:
Skouridou, Vasso; Tomaso, Herbert; Rau, Jörg; Bashammakh, Abdulaziz S.; El-Shahawi, Mohammad S.; Alyoubi, Abdulrahman O.; O'Sullivan, Ciara K.
Source:
Food chemistry 2019
ISSN:
0308-8146
Subject:
analytical kits,   etc   ; DNA; adulterants; adulterated products; beef; chicken meat; colorimetry; cost effectiveness; hybridization; oligonucleotides; polymerase chain reaction; pork; raw meat;     Show all 13 Subjects
Abstract:
... In this work, a duplex PCR–Enzyme Linked Oligonucleotide Assay (ELONA) is reported for the sensitive and reliable detection of pork adulteration in beef and chicken products, two of the most widely consumed meat types in the world. The strategy relies on the use of species-specific tailed primers for duplex amplification and simple dilution of the PCR reactions for direct colorimetric detection vi ...
DOI:
10.1016/j.foodchem.2019.02.095

14. Polymeric fluorescent heparin as one-step FRET substrate of human heparanase

Author:
Sistla, Jyothi C.; Morla, Shravan; Alabbas, Al-Humaidi B.; Kalathur, Ravi C.; Sharon, Chetna; Patel, Bhaumik B.; Desai, Umesh R.
Source:
Carbohydrate polymers 2019 v.205 pp. 385-391
ISSN:
0144-8617
Subject:
analytical kits,   etc   ; beta-glucuronidase; cell growth; cell lines; extracellular matrix; fluorescence; heparan sulfate; heparin; humans; labor; metastasis; screening;     Show all 12 Subjects
Abstract:
... Heparanase, an endo-β-D-glucuronidase, cleaves cell surface and extracellular matrix heparan sulfate (HS) chains and plays important roles in cellular growth and metastasis. Heparanase assays reported to-date are labor intensive, complex and/or expensive. A simpler assay is critically needed to understand the myriad roles of heparanase. We reasoned that fluorescent heparin could serve as an effect ...
DOI:
10.1016/j.carbpol.2018.10.071

15. Association of SELP Polymorphisms with Soluble P-Selectin Levels and Vascular Risk in Patients with Type 2 Diabetes Mellitus: A Case–Control Study

Author:
Kaur, Raminderjit; Singh, Jatinder; Kapoor, Rohit; Kaur, Manpreet
Source:
Biochemical genetics 2019 v.57 no.1 pp. 73-97
ISSN:
0006-2928
Subject:
analytical kits,   etc   ; alleles; biomarkers; case-control studies; computer software; haplotypes; heterozygosity; homozygosity; patients; protective effect; risk; statistical analysis;     Show all 12 Subjects
Abstract:
... P-selectin, an adhesion molecule, is encoded by SELP and known as biomarker of endothelial as well as platelet dysfunction. SELP polymorphisms (rs6136, rs6127, and rs6125) and raised levels of soluble P-selectin (sP-selectin) have been associated with several disease conditions. The present study was aimed to determine the association of SELP variants and sP-selectin levels as well as vascular ris ...
DOI:
10.1007/s10528-018-9881-6

16. Direct comparison of QIAamp DSP Virus Kit and QIAamp Circulating Nucleic Acid Kit regarding cell-free fetal DNA isolation from maternal peripheral blood

Author:
Jain, Mark; Balatsky, Alexander Vladimirovich; Revina, Daria Borisovna; Samokhodskaya, Larisa Mikhailovna
Source:
Molecular and cellular probes 2019 v.43 pp. 13-19
ISSN:
0890-8508
Subject:
analytical kits,   etc   ; DNA; blood; blood sampling; laboratory equipment; markets; polymerase chain reaction; pregnancy; pregnant women; qualitative analysis; quantitative analysis; viruses;     Show all 12 Subjects
Abstract:
... Blood of pregnant women contains cell-free fetal DNA (cffDNA), which is widely used in non-invasive prenatal diagnosis. The modern laboratory equipment market provides huge variety of commercial kits for isolation of circulating nucleic acids, but unfortunately none of them are standardized for isolation of cffDNA, which is a crucial step for success of subsequent analysis.To compare DSPVK and CNA ...
DOI:
10.1016/j.mcp.2018.12.006

17. Development and evaluation of an improved quantitative loop-mediated isothermal amplification method for rapid detection of Morganella morganii

Author:
Shahbazi, Erfan; Mollasalehi, Hamidreza; Minai-Tehrani, Dariush
Source:
Talanta 2019 v.191 pp. 54-58
ISSN:
0039-9140
Subject:
analytical kits,   etc   ; Morganella morganii; antigens; bacteria; betaine; dimethyl sulfoxide; loop-mediated isothermal amplification; point-of-care systems; rapid methods; turbidity;     Show all 10 Subjects
Abstract:
... A point-of-care diagnostic kit was developed for detection of Morganella morganii using optimized loop-mediated isothermal amplification (LAMP) technique within less than an hour. In that regard, dimethyl sulfoxide (DMSO) was utilized together with betaine and all variables were optimized to improve the efficiency of the method. Moreover, surface presentation of antigens protein was targeted and s ...
DOI:
10.1016/j.talanta.2018.08.033

18. Development of rapid dipstick assay for food pathogens, Salmonella, by optimized parameters

Author:
Çam, Dilek; Öktem, Hüseyin Avni
Source:
Journal of food science and technology 2019 v.56 no.1 pp. 140-148
ISSN:
0022-1155
Subject:
analytical kits,   etc   ; Salmonella; animal and human health; antibodies; detection limit; food pathogens; heat inactivation; immunoaffinity chromatography; nanogold;     Show all 9 Subjects
Abstract:
... Salmonella is among the very important pathogens threating the human and animal health. Rapid and easy detection of these pathogens is crucial. In this context, antibody (Ab) based lateral flow assays (LFAs) which are simple immunochromatographic point of care test kits were developed by gold nanoparticles (GNPs) as labelling agent for Salmonella detection. For that purpose some critical parameter ...
DOI:
10.1007/s13197-018-3467-5

19. Comparison of eight commercially available kits for DNA extraction from formalin-fixed paraffin-embedded tissues

Author:
Janecka, Anna; Adamczyk, Agnieszka; Gasińska, Anna
Source:
Analytical biochemistry 2015 v.476 pp. 8-10
ISSN:
0003-2697
Subject:
analytical kits,   etc   ; DNA; tissues;     Show all 3 Subjects
Abstract:
... A proper extraction method from formalin-fixed paraffin-embedded (FFPE) blocks is essential to obtain DNA of satisfactory quality/quantity. We compared the effectiveness of eight commercially available kits for DNA extraction based on 10 FFPE tissues. Kits differed significantly in terms of DNA yield, purity, and quality. Using the QIAamp DNA FFPE Tissue Kit (Qiagen) and the ReliaPrep FFPE gDNA Mi ...
DOI:
10.1016/j.ab.2015.01.019

20. Prevalence of Borrelia burgdorferi, Anaplasma spp., Ehrlichia spp. and Dirofilaria immitis in Canadian dogs, 2008 to 2015: a repeat cross-sectional study

Author:
Evason, Michelle; Stull, Jason W.; Pearl, David L.; Peregrine, Andrew S.; Jardine, Claire; Buch, Jesse S.; Lailer, Zachary; O’Connor, Tom; Chandrashekar, Ramaswamy; Weese, J. Scott
Source:
Parasites & vectors 2019 v.12 no.1 pp. 64
ISSN:
1756-3305
Subject:
analytical kits,   etc   ; Anaplasma phagocytophilum; Borrelia burgdorferi; Dirofilaria immitis; Ehrlichia canis; Lyme disease; antigens; cross-sectional studies; dogs; geographical variation; mixed infection; pathogens; risk reduction; serology; seroprevalence; Canada;     Show all 16 Subjects
Abstract:
... BACKGROUND: Vector-borne pathogens are emerging concerns in multiple regions of Canada. Determining regional prevalence of canine vector-borne pathogens and documenting change will improve clinician awareness, enable targeted prevention, enhance diagnosis and ideally reduce the risk of disease. Study objectives were to: (i) estimate the prevalence of positive canine vector-borne test results from ...
DOI:
10.1186/s13071-019-3299-9