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1. Diagnostic kit based on halloysite nanoclay-ionic liquid nanocomposite modified electrode for electrochemical determination of cancer biomarker

Author:
Esma Yildiz; Beste Yurdacan; Yasemin Erac; Arzum Erdem
Source:
Talanta 2023 v.252 pp. 123854
ISSN:
0039-9140
Subject:
analytical kits,   etc   ; DNA probes; RNA; biomarkers; breast neoplasms; carbon electrodes; cell lines; detection limit; dielectric spectroscopy; electrochemistry; energy-dispersive X-ray analysis; halloysite; humans; hybridization; inosine; kidneys; liquids; nanocomposites; neoplasm cells; nucleic acid hybridization; nucleotide sequences; voltammetry;     Show all 22 Subjects
Abstract:
... Nucleic acid hybridization is occurred between the selective single-stranded nucleic acid sequence and its target sequence, which is one of the essential procedure for electrochemical detection of nucleic acid. microRNA-21 (miRNA-21) is known as a biomarker in various cancers. The determination of miRNA-21 was attained through by hybridization of inosine substituted miRNA-21 specific DNA probe (Pᵢ ...
DOI:
10.1016/j.talanta.2022.123854

2. Diagnosis of osteoarticular tuberculosis by immuno‐PCR assay based on mycobacterial antigen 85 complex detection

Author:
A. Khan; R. Singh; S. Sharma; V. Singh; A. Sheoran; A. Soni; V. Dhull; P.S. Gill; A. Yadav; D. Chaudhary; M.C. Gupta; P.K. Mehta
Source:
Letters in applied microbiology 2022 v.74 no.1 pp. 17-26
ISSN:
0266-8254
Subject:
analytical kits,   etc   ; antigens; microbiology; tuberculosis;     Show all 4 Subjects
Abstract:
... Diagnosis of osteoarticular tuberculosis (OATB) exhibits serious challenges owing to paucibacillary nature of specimens and localization of disease at sites that are difficult to access. We recently developed indirect immuno‐PCR (I‐PCR) and real‐time I‐PCR (RT‐I‐PCR) assays for the detection of mycobacterial antigen 85 complex (Ag85) in OATB patients. Detection limits for the purified Ag85 protein ...
DOI:
10.1111/lam.13567

3. Ultrasensitive ELISA detection of proteins in separated lumen and membrane fractions of cancer cell exosomes

Author:
Kanako Iha; Naoko Tsurusawa; Hsin-Yi Tsai; Ming-Wei Lin; Hikaru Sonoda; Satoshi Watabe; Teruki Yoshimura; Etsuro Ito
Source:
Analytical biochemistry 2022 v.654 pp. 114831
ISSN:
0003-2697
Subject:
analytical kits,   etc   ; exosomes; neoplasm cells; neoplasm progression; neoplasms;     Show all 5 Subjects
Abstract:
... Exosomes transfer molecules horizontally to surrounding cells and therefore have a key role in cancer progression. To clarify the role of exosomes in cancer progression, trace amounts of proteins in their lumen and membrane fractions should be analyzed separately. For this purpose, an adequate and easy-to-use method of separating the lumen and membrane fractions of exosomes must be developed. Furt ...
DOI:
10.1016/j.ab.2022.114831

4. Third-generation sequencing: A novel tool detects complex variants in the α-thalassemia gene

Author:
Ju Long; Lei Sun; Feifei Gong; Chenghong Zhang; Aiping Mao; Yulin Lu; Jiaqi Li; Enqi Liu
Source:
Gene 2022 v.822 pp. 146332
ISSN:
0378-1119
Subject:
analytical kits,   etc   ; DNA; genetic analysis; hematology; multigene family; mutation; thalassemia; China;     Show all 8 Subjects
Abstract:
... Thalassemia is a monogenic disorder with a high carrier rate in the southern region of China. Most laboratories currently follow the protocol of testing hematologic indicators in individuals with positive hematologic indicators and then using the hot-spot mutation test kit. A novel thalassemia gene test is performed if there is a mismatch between the hematology and hot-spot mutation test results. ...
DOI:
10.1016/j.gene.2022.146332

5. Evaluation of the PrioCHECK™ Trichinella AAD kit to detect Trichinella spiralis, T. britovi, and T. pseudospiralis larvae in pork using the automated digestion method Trichomatic-35

Author:
Walter Basso; Nelson Marreros; Larissa Hofmann; Christine Salvisberg; Britta Lundström-Stadelmann; Caroline F. Frey
Source:
Parasitology international 2022 v.86 pp. 102449
ISSN:
1383-5769
Subject:
analytical kits,   etc   ; Trichinella spiralis; automation; digestion; magnetism; parasitology; pork; trichinosis; zoonoses;     Show all 9 Subjects
Abstract:
... Trichinellosis is a potentially deadly parasitic zoonosis that is contracted by consuming undercooked infected meat. Reliable detection of infectious Trichinella spp. larvae in meat is therefore pivotal to ensure consumer's safety. The recently authorised PrioCHECK™ Trichinella Alternative Artificial Digestion (AAD) test kit appears promising when used with the standard magnetic stirrer method, bu ...
DOI:
10.1016/j.parint.2021.102449

6. Highly sensitive pregnancy test kit via oriented antibody conjugation on brush-type ligand-coated quantum beads

Author:
Sunghwan Kim; Yujin Ahn; Yoonji Bae; Sunyoung Woo; Jungsik Park; Im kyung Han; Hyunhong Kim; Soomin Eom; Sebyung Kang; Woonggyu Jung; Jongnam Park
Source:
Biosensors & bioelectronics 2022 v.213 pp. 114441
ISSN:
0956-5663
Subject:
analytical kits,   etc   ; antibodies; biosensors; detection limit; heart diseases; ligands; malaria; pregnancy;     Show all 8 Subjects
Abstract:
... Lateral flow assays (LFA) enable development of portable and rapid diagnostic kits; however, their capacity to detect low levels of disease markers remains poor. Here, we report a highly sensitive pregnancy test kit as a proof of concept, by combining brush-type ligand-coated quantum beads (B-type QBs) and nanobody, which can control the antibody orientation and enhance sensitivity. The brush-type ...
DOI:
10.1016/j.bios.2022.114441

7. Impact assessment of different DNA extraction methods for non-invasive molecular diagnosis of tegumentary leishmaniasis

Author:
Verônica Cardoso Santos de Faria; Denise Utsch Gonçalves; Arthur Ribeiro Cheloni Soares; Pedro Henrique Barbosa; Juliana Wilke Saliba; Carolina Senra Alves de Souza; Gláucia Fernandes Cota; Daniel Moreira de Avelar
Source:
Acta tropica 2022 v.227 pp. 106275
ISSN:
0001-706X
Subject:
analytical kits,   etc   ; DNA; biopsy; extracts; leishmaniasis; patients; polymerase chain reaction; sampling; testing;     Show all 9 Subjects
Abstract:
... The aim of this study was to evaluate two methods of nucleic acid extraction (spin-column-based method – commercial kit and direct boil - DB) from swab sampling compared to biopsy sampling for the diagnosis of tegumentary leishmaniasis (TL), (cutaneous - CL and mucocutaneous - MCL forms). The impact of these nucleic acid extraction protocols on different types of PCR and LAMP techniques were compa ...
DOI:
10.1016/j.actatropica.2021.106275

8. A paper-based microfluidic sensor array combining molecular imprinting technology and carbon quantum dots for the discrimination of nitrophenol isomers

Author:
Liang Zhu; Xuecui Mei; Zhengchun Peng; Jiao Yang; Yingchun Li
Source:
Journal of hazardous materials 2022 v.435 pp. 129012
ISSN:
0304-3894
Subject:
analytical kits,   etc   ; blue light; fluorescence; food safety; glass fibers; molecular imprinting; prognosis;     Show all 7 Subjects
Abstract:
... Paper-based microfluidic analytical devices (μPADs) have recently attracted attention as a rapid test kit owing to their low cost and nonrequirement for external driving pump. However, low accuracy and poor anti-interference ability of μPADs under complex detection condition limit their practical applications. Here, we present a facile way to prepare a novel fluorescence sensor-array μPAD for mult ...
DOI:
10.1016/j.jhazmat.2022.129012

9. A self-pressure-driven blood plasma-separation device for point-of-care diagnostics

Author:
Hanbi Kim; Hyeonseek Park; Doo Ryeon Chung; Taekyung Kim; Eunkyoung Park; Minhee Kang
Source:
Talanta 2022 v.247 pp. 123562
ISSN:
0039-9140
Subject:
analytical kits,   etc   ; blood; design; exercise test; hematologic tests; point-of-care systems; sampling;     Show all 7 Subjects
Abstract:
... We aimed to develop a portable, simple-to-use, and self-pressure-driven blood plasma-separation device that can be combined with rapid diagnostic test kits. This simple, disposable, and electrical equipment-free apparatus has been designed to separate plasma from a few microliters of blood with only hand-powered operation. The refined plasma sample is then delivered to multiple lateral flow assay ...
DOI:
10.1016/j.talanta.2022.123562

10. Assessment of three DNA extraction kits for the absolute quantification of strongyle nematode eggs in faecal samples

Author:
Niclas Högberg; Paulius Baltrušis; Nizar Enweji; Johan Höglund
Source:
Acta veterinaria scandinavica 2022 v.64 no.1 pp. 5
ISSN:
1751-0147
Subject:
analytical kits,   etc   ; DNA; Haemonchus contortus; droplets; feces; gastrointestinal nematodes; polymerase chain reaction; prices;     Show all 8 Subjects
Abstract:
... BACKGROUND: Haemonchus contortus is one of the most pathogenic gastrointestinal nematodes of small ruminants. The current diagnostic approach for the detection of this species relies on coproscopic methods, which both have low sensitivity and are time consuming. Methods employing detection through DNA amplification, such as droplet digital polymerase chain reaction (ddPCR), offer an advantageous a ...
DOI:
10.1186/s13028-022-00624-3

11. An Assessment of Cataract Severity Based on Antioxidant Status and Ascorbic Acid Levels in Aqueous Humor

Author:
Yu-Ting Tsao; Wei-Chi Wu; Kuan-Jen Chen; Chun-Fu Liu; Yi-Jen Hsueh; Chao-Min Cheng; Hung-Chi Chen
Source:
Antioxidants 2022 v.11 no.2 pp. -
ISSN:
2076-3921
Subject:
analytical kits,   etc   ; antioxidant activity; antioxidants; ascorbic acid; blindness; cataract; energy; guidelines; quantitative analysis; regression analysis;     Show all 10 Subjects
Abstract:
... Cataract is the leading cause of blindness throughout the world. Currently, the cataract severity evaluation is based on the subjective LOCS III guideline. To ameliorate the evaluation system and develop an objective and quantitative analysis, we investigated the relationships among aqueous humor total antioxidant capacity (AqTAC), ascorbic acid (AqAA) concentration, and cataract severity. In this ...
DOI:
10.3390/antiox11020397

12. Immunoglobulin G Concentrations in Alpaca Colostrum during the First Four Days after Parturition

Author:
Maria Mößler; Kathrin Rychli; Volker Michael Reichmann; Thiemo Albert; Thomas Wittek
Source:
Animals 2022 v.12 no.2 pp. -
ISSN:
2076-2615
Subject:
analytical kits,   etc   ; Camelidae; alpacas; cobalt; colostrum; immune system; immunoglobulin G; intestines; lactose; magnesium; neonates; parturition;     Show all 12 Subjects
Abstract:
... Colostrum provides the newborn with nutrients and immunoglobulins. Immunoglobulins and their intestinal transfer play a major role in the immune system of neonates since they are born agammaglobulinemic. In this study immunoglobulin G (IgG) content was determined in alpaca colostrum and the correlations of the IgG concentration by fat, protein, lactose and minerals were calculated. Colostrum sampl ...
DOI:
10.3390/ani12020167

13. Simple and green colorimetric method for the detection of formaldehyde in vegetable samples

Author:
Yanisa Thepchuay; Wannisa Chairit; Naengnoi Saengsane; Paweena Porrawatkul; Rungnapa Pimsen
Source:
Journal of food composition and analysis 2022 v.111 pp. 104623
ISSN:
0889-1575
Subject:
analytical kits,   etc   ; color; colorimetry; food composition; formaldehyde; formic acid; hydrogen peroxide; oxidation; ultraviolet-visible spectroscopy; vegetables;     Show all 10 Subjects
Abstract:
... This work presented a simple, rapid, low-cost, and green chemical approach for the quantification of formaldehyde in vegetable samples. The detection of formaldehyde depends on its oxidation with hydrogen peroxide in an alkaline medium to form formic acid, resulting in an indicator color change from blue to yellow observed by the UV-Vis spectrophotometer or even the naked eye. Under the optimal pa ...
DOI:
10.1016/j.jfca.2022.104623

14. A direct contact bioassay using immobilized microalgal balls to evaluate the toxicity of contaminated field soils

Author:
Fida Hussain; Ebenezer Ashun; Sokhee P. Jung; Taeyoung Kim; Sang-Hun Lee; Dong-Jin Kim; Sang-Eun Oh
Source:
Journal of environmental management 2022 v.321 pp. 115930
ISSN:
0301-4797
Subject:
analytical kits,   etc   ; Chlorella vulgaris; bioassays; direct contact; environmental management; headspace analysis; microalgae; oxygen; photosynthesis; soil toxicity; toxicity;     Show all 11 Subjects
Abstract:
... The present study used a bioassay of immobilized microalgae (Chlorella vulgaris) via direct contact to assess the toxicity of eleven uncontaminated (reference) and five field contaminated soils with various physicochemical properties and contamination. Photosynthetic oxygen concentration in the headspace of the test kit by Chlorella vulgaris in the reference soils ranged between 12.93% and 14.80% ...
DOI:
10.1016/j.jenvman.2022.115930

15. Donor-σ-Acceptor Dyad-Based Polymers for Portable Sensors: Controlling Photoinduced Electron Transfer via Tuning the Frontier Molecular Orbital Energies of Acceptors

Author:
Na Yeon Kwon; Youngseo Kim; Meenal Kataria; Su Hong Park; Seunguk Cho; Amit Kumar Harit; Han Young Woo; Min Ju Cho; Sungnam Park; Dong Hoon Choi
Source:
Macromolecules 2022 v.55 no.5 pp. 1609-1619
ISSN:
1520-5835
Subject:
analytical kits,   etc   ; absorption; detection limit; electrochemistry; electron transfer; fermented foods; fluorescence; moieties; naphthalene; polymers; spermine; urine; vapors; voltammetry;     Show all 14 Subjects
Abstract:
... In this study, we synthesized three novel aromatic imide-based conjugated donor(D)-σ-acceptor (A) dyad-based polymers showing different photophysical properties. These D-σ-A dyad-based polymers (e.g., BDTBT-NI, BDTBT-NDI, and BDTBT-PDI) consist of the same conjugated donor main chains (benzodithiophene-bithiophene (BDTBT)) and side chains bearing different imide acceptors (naphthalimide (NI), naph ...
DOI:
10.1021/acs.macromol.1c02532

16. Diagnosis of urogenital tuberculosis by multiplex‐nested PCR targeting mpt64 (Rv1980c) and IS6110: comparison with multiplex PCR and GeneXpert® MTB/RIF

Author:
E. Kamra; D. Alam; V. Singh; M. Kumar; M. Chauhan; P.K. Mehta
Source:
Letters in applied microbiology 2022 v.75 no.4 pp. 857-868
ISSN:
0266-8254
Subject:
analytical kits,   etc   ; DNA; Mycobacterium tuberculosis; blood; detection limit; endometrium; females; genes; humans; males; microbiology; polymerase chain reaction; tuberculosis; urine;     Show all 14 Subjects
Abstract:
... A multiplex‐nested PCR (M‐nested PCR) targeting mpt64 (Rv1980c) + IS6110 was designed to detect Mycobacterium tuberculosis (Mtb) DNA within urine (n = 35), endometrial biopsies (n = 22) and menstrual blood (n = 3) of male/female UGTB patients, and results were compared with M‐PCR using the same targets. Detection limit of the purified Mtb DNA was found to be 1 fg by M‐nested PCR, which was 10⁶‐fol ...
DOI:
10.1111/lam.13758

17. Detection and differentiation of Salmonella Enteritidis and Salmonella Typhimurium by multiplex quantitative PCR from different poultry matrices

Author:
İ. B. Müştak; H. K. Müştak
Source:
British poultry science 2022 v.63 no.2 pp. 171-178
ISSN:
1466-1799
Subject:
analytical kits,   etc   ; Salmonella Enteritidis; Salmonella Typhimurium; chicken meat; cloaca; detection limit; genes; infrastructure; poultry; quantitative polymerase chain reaction; serotypes;     Show all 11 Subjects
Abstract:
... 1. The aim of this study was to develop a multiplex quantitative polymerase chain reaction (qPCR) based molecular diagnostic kit for rapid diagnosis of Salmonella enterica serovar Enteritidis and S. enterica serovar Typhimurium serotypes, which are frequently isolated worldwide from poultry samples. 2. Detection and discrimination of S. Enteritidis and S. Typhimurium were performed by targeting th ...
DOI:
10.1080/00071668.2021.1966751

18. Polypeptide induced perylene probe excimer formation and its application in the noncovalent ratiometric detection of matrix metalloproteinase activity

Author:
Xiaoyu Gou; Muhammad Azhar Hayat Nawaz; Chaoyi Liu; Na Yang; Jia Ren; Huipeng Zhou; Yunhui Li; Jianwei Zhu; Wenzhao Han; Cong Yu
Source:
Journal of materials chemistry B 2022 v.10 no.30 pp. 5774-5783
ISSN:
2050-7518
Subject:
analytical kits,   etc   ; apigenin; biomarkers; chemistry; detection limit; enzyme activity; fluorescence; fluorescent dyes; humans; metalloproteinases; metastasis; neoplasm cells; polypeptides; quercetin; saliva;     Show all 15 Subjects
Abstract:
... Matrix metalloproteinases (MMPs) are important biomarkers for a number of diseases. Thus, the precise determination of MMP activity is of crucial importance. Herein, we report a ratiometric fluorescence method for the sensitive and selective sensing of MMP activity. A number of positively charged MMP substrates (polypeptides) were designed and prepared. These polypeptides could induce aggregation ...
DOI:
10.1039/d2tb00416j

19. Seroprevalence of Anti-Theileria equi Antibodies in Horses from Three Geographically Distinct Areas of Romania

Author:
Simona Giubega; Marius Stelian Ilie; Iasmina Luca; Tiana Florea; Cristian Dreghiciu; Ion Oprescu; Sorin Morariu; Gheorghe Dărăbuș
Source:
Pathogens 2022 v.11 no.6 pp. -
ISSN:
2076-0817
Subject:
analytical kits,   etc   ; Babesia caballi; Equidae; Theileria equi; antibodies; blood serum; equine piroplasmosis; geographical distribution; horses; mixed breeds; risk; seroprevalence; Romania;     Show all 13 Subjects
Abstract:
... Equine piroplasmosis (EP) is an endemic tick-borne disease found in most countries around the world. It affects all species of Equidae, and it is caused by Theileria equi, Babesia caballi and T. haneyi. The research herein is the second study on the prevalence of piroplasms in Romania conducted in the past two decades. The aim of this study was to assess the seroprevalence of anti-Theileria equi a ...
DOI:
10.3390/pathogens11060669

20. Effect of high nitrate vegetable juice supplementation on plasma nitrate and blood pressure in adults: a pilot randomized crossover intervention in healthy volunteers

Author:
Nour A. Elsahoryi; Christopher Cardwell; Sarah Gilchrist; Jayne V. Woodside
Source:
Journal of nutritional science 2022 v.11 pp. e41
ISSN:
2048-6790
Subject:
analytical kits,   etc   ; beets; cross-over studies; diastolic blood pressure; green leafy vegetables; nitrates; nitrites; systolic blood pressure; t-test; vegetable juices;     Show all 10 Subjects
Abstract:
... Beetroot juice (BRJ) has been demonstrated to decrease blood pressure (BP) due to the high inorganic nitrate content. This pilot randomized crossover trial aimed to investigate the effect of two different high nitrate vegetable juices on plasma nitrate concentrations and BP in healthy adults. Eighteen healthy volunteers were randomized to receive 115 ml of BRJ or 250 ml of green leafy vegetable ju ...
DOI:
10.1017/jns.2022.34