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analyticalkits, etc ; DNA probes; RNA; biomarkers; breast neoplasms; carbon electrodes; cell lines; detection limit; dielectric spectroscopy; electrochemistry; energy-dispersive X-ray analysis; halloysite; humans; hybridization; inosine; kidneys; liquids; nanocomposites; neoplasm cells; nucleic acid hybridization; nucleotide sequences; voltammetry; Show all 22 Subjects
Abstract:
... Nucleic acid hybridization is occurred between the selective single-stranded nucleic acid sequence and its target sequence, which is one of the essential procedure for electrochemical detection of nucleic acid. microRNA-21 (miRNA-21) is known as a biomarker in various cancers. The determination of miRNA-21 was attained through by hybridization of inosine substituted miRNA-21 specific DNA probe (Pᵢ ...
analyticalkits, etc ; antigens; microbiology; tuberculosis; Show all 4 Subjects
Abstract:
... Diagnosis of osteoarticular tuberculosis (OATB) exhibits serious challenges owing to paucibacillary nature of specimens and localization of disease at sites that are difficult to access. We recently developed indirect immuno‐PCR (I‐PCR) and real‐time I‐PCR (RT‐I‐PCR) assays for the detection of mycobacterial antigen 85 complex (Ag85) in OATB patients. Detection limits for the purified Ag85 protein ...
analyticalkits, etc ; exosomes; neoplasm cells; neoplasm progression; neoplasms; Show all 5 Subjects
Abstract:
... Exosomes transfer molecules horizontally to surrounding cells and therefore have a key role in cancer progression. To clarify the role of exosomes in cancer progression, trace amounts of proteins in their lumen and membrane fractions should be analyzed separately. For this purpose, an adequate and easy-to-use method of separating the lumen and membrane fractions of exosomes must be developed. Furt ...
analyticalkits, etc ; DNA; genetic analysis; hematology; multigene family; mutation; thalassemia; China; Show all 8 Subjects
Abstract:
... Thalassemia is a monogenic disorder with a high carrier rate in the southern region of China. Most laboratories currently follow the protocol of testing hematologic indicators in individuals with positive hematologic indicators and then using the hot-spot mutation test kit. A novel thalassemia gene test is performed if there is a mismatch between the hematology and hot-spot mutation test results. ...
analyticalkits, etc ; Trichinella spiralis; automation; digestion; magnetism; parasitology; pork; trichinosis; zoonoses; Show all 9 Subjects
Abstract:
... Trichinellosis is a potentially deadly parasitic zoonosis that is contracted by consuming undercooked infected meat. Reliable detection of infectious Trichinella spp. larvae in meat is therefore pivotal to ensure consumer's safety. The recently authorised PrioCHECK™ Trichinella Alternative Artificial Digestion (AAD) test kit appears promising when used with the standard magnetic stirrer method, bu ...
analyticalkits, etc ; antibodies; biosensors; detection limit; heart diseases; ligands; malaria; pregnancy; Show all 8 Subjects
Abstract:
... Lateral flow assays (LFA) enable development of portable and rapid diagnostic kits; however, their capacity to detect low levels of disease markers remains poor. Here, we report a highly sensitive pregnancy test kit as a proof of concept, by combining brush-type ligand-coated quantum beads (B-type QBs) and nanobody, which can control the antibody orientation and enhance sensitivity. The brush-type ...
Verônica Cardoso Santos de Faria; Denise Utsch Gonçalves; Arthur Ribeiro Cheloni Soares; Pedro Henrique Barbosa; Juliana Wilke Saliba; Carolina Senra Alves de Souza; Gláucia Fernandes Cota; Daniel Moreira de Avelar
analyticalkits, etc ; DNA; biopsy; extracts; leishmaniasis; patients; polymerase chain reaction; sampling; testing; Show all 9 Subjects
Abstract:
... The aim of this study was to evaluate two methods of nucleic acid extraction (spin-column-based method – commercial kit and direct boil - DB) from swab sampling compared to biopsy sampling for the diagnosis of tegumentary leishmaniasis (TL), (cutaneous - CL and mucocutaneous - MCL forms). The impact of these nucleic acid extraction protocols on different types of PCR and LAMP techniques were compa ...
analyticalkits, etc ; blue light; fluorescence; food safety; glass fibers; molecular imprinting; prognosis; Show all 7 Subjects
Abstract:
... Paper-based microfluidic analytical devices (μPADs) have recently attracted attention as a rapid test kit owing to their low cost and nonrequirement for external driving pump. However, low accuracy and poor anti-interference ability of μPADs under complex detection condition limit their practical applications. Here, we present a facile way to prepare a novel fluorescence sensor-array μPAD for mult ...
analyticalkits, etc ; blood; design; exercise test; hematologic tests; point-of-care systems; sampling; Show all 7 Subjects
Abstract:
... We aimed to develop a portable, simple-to-use, and self-pressure-driven blood plasma-separation device that can be combined with rapid diagnostic test kits. This simple, disposable, and electrical equipment-free apparatus has been designed to separate plasma from a few microliters of blood with only hand-powered operation. The refined plasma sample is then delivered to multiple lateral flow assay ...
analyticalkits, etc ; DNA; Haemonchus contortus; droplets; feces; gastrointestinal nematodes; polymerase chain reaction; prices; Show all 8 Subjects
Abstract:
... BACKGROUND: Haemonchus contortus is one of the most pathogenic gastrointestinal nematodes of small ruminants. The current diagnostic approach for the detection of this species relies on coproscopic methods, which both have low sensitivity and are time consuming. Methods employing detection through DNA amplification, such as droplet digital polymerase chain reaction (ddPCR), offer an advantageous a ...
analyticalkits, etc ; antioxidant activity; antioxidants; ascorbic acid; blindness; cataract; energy; guidelines; quantitative analysis; regression analysis; Show all 10 Subjects
Abstract:
... Cataract is the leading cause of blindness throughout the world. Currently, the cataract severity evaluation is based on the subjective LOCS III guideline. To ameliorate the evaluation system and develop an objective and quantitative analysis, we investigated the relationships among aqueous humor total antioxidant capacity (AqTAC), ascorbic acid (AqAA) concentration, and cataract severity. In this ...
analyticalkits, etc ; Camelidae; alpacas; cobalt; colostrum; immune system; immunoglobulin G; intestines; lactose; magnesium; neonates; parturition; Show all 12 Subjects
Abstract:
... Colostrum provides the newborn with nutrients and immunoglobulins. Immunoglobulins and their intestinal transfer play a major role in the immune system of neonates since they are born agammaglobulinemic. In this study immunoglobulin G (IgG) content was determined in alpaca colostrum and the correlations of the IgG concentration by fat, protein, lactose and minerals were calculated. Colostrum sampl ...
analyticalkits, etc ; color; colorimetry; food composition; formaldehyde; formic acid; hydrogen peroxide; oxidation; ultraviolet-visible spectroscopy; vegetables; Show all 10 Subjects
Abstract:
... This work presented a simple, rapid, low-cost, and green chemical approach for the quantification of formaldehyde in vegetable samples. The detection of formaldehyde depends on its oxidation with hydrogen peroxide in an alkaline medium to form formic acid, resulting in an indicator color change from blue to yellow observed by the UV-Vis spectrophotometer or even the naked eye. Under the optimal pa ...
analyticalkits, etc ; Chlorella vulgaris; bioassays; direct contact; environmental management; headspace analysis; microalgae; oxygen; photosynthesis; soil toxicity; toxicity; Show all 11 Subjects
Abstract:
... The present study used a bioassay of immobilized microalgae (Chlorella vulgaris) via direct contact to assess the toxicity of eleven uncontaminated (reference) and five field contaminated soils with various physicochemical properties and contamination. Photosynthetic oxygen concentration in the headspace of the test kit by Chlorella vulgaris in the reference soils ranged between 12.93% and 14.80% ...
analyticalkits, etc ; absorption; detection limit; electrochemistry; electron transfer; fermented foods; fluorescence; moieties; naphthalene; polymers; spermine; urine; vapors; voltammetry; Show all 14 Subjects
Abstract:
... In this study, we synthesized three novel aromatic imide-based conjugated donor(D)-σ-acceptor (A) dyad-based polymers showing different photophysical properties. These D-σ-A dyad-based polymers (e.g., BDTBT-NI, BDTBT-NDI, and BDTBT-PDI) consist of the same conjugated donor main chains (benzodithiophene-bithiophene (BDTBT)) and side chains bearing different imide acceptors (naphthalimide (NI), naph ...
... A multiplex‐nested PCR (M‐nested PCR) targeting mpt64 (Rv1980c) + IS6110 was designed to detect Mycobacterium tuberculosis (Mtb) DNA within urine (n = 35), endometrial biopsies (n = 22) and menstrual blood (n = 3) of male/female UGTB patients, and results were compared with M‐PCR using the same targets. Detection limit of the purified Mtb DNA was found to be 1 fg by M‐nested PCR, which was 10⁶‐fol ...
... 1. The aim of this study was to develop a multiplex quantitative polymerase chain reaction (qPCR) based molecular diagnostic kit for rapid diagnosis of Salmonella enterica serovar Enteritidis and S. enterica serovar Typhimurium serotypes, which are frequently isolated worldwide from poultry samples. 2. Detection and discrimination of S. Enteritidis and S. Typhimurium were performed by targeting th ...
... Matrix metalloproteinases (MMPs) are important biomarkers for a number of diseases. Thus, the precise determination of MMP activity is of crucial importance. Herein, we report a ratiometric fluorescence method for the sensitive and selective sensing of MMP activity. A number of positively charged MMP substrates (polypeptides) were designed and prepared. These polypeptides could induce aggregation ...
... Equine piroplasmosis (EP) is an endemic tick-borne disease found in most countries around the world. It affects all species of Equidae, and it is caused by Theileria equi, Babesia caballi and T. haneyi. The research herein is the second study on the prevalence of piroplasms in Romania conducted in the past two decades. The aim of this study was to assess the seroprevalence of anti-Theileria equi a ...
analyticalkits, etc ; beets; cross-over studies; diastolic blood pressure; green leafy vegetables; nitrates; nitrites; systolic blood pressure; t-test; vegetable juices; Show all 10 Subjects
Abstract:
... Beetroot juice (BRJ) has been demonstrated to decrease blood pressure (BP) due to the high inorganic nitrate content. This pilot randomized crossover trial aimed to investigate the effect of two different high nitrate vegetable juices on plasma nitrate concentrations and BP in healthy adults. Eighteen healthy volunteers were randomized to receive 115 ml of BRJ or 250 ml of green leafy vegetable ju ...