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1. Locally produced autoantibodies in cancer

Author:
Sara M. Centuori; Deepta Bhattacharya
Source:
Cell 2022 v.185 no.7 pp. 1110-1111
ISSN:
0092-8674
Subject:
antibody formation,   etc   ; B-lymphocytes; autoantibodies; metalloproteinases;     Show all 4 Subjects
Abstract:
... The function and antigen-specificities of tumor-infiltrating B cells are mostly unknown. In a new study by Mazor et al., matrix metalloproteinase 14 (MMP14), a self-antigen that is overexpressed by ovarian cancers, is shown to drive B cell activation and autoantibody production in tertiary lymphoid structures (Mazor et al., 2022). ...
DOI:
10.1016/j.cell.2022.03.006

2. Immunogenicity of fractional-dose of inactivated poliomyelitis vaccine made from Sabin strains delivered by intradermal vaccination in Wistar rats

Author:
Yan Ma; Zhifang Ying; Jingyan Li; Qin Gu; Xiaoyu Wang; Lukui Cai; Li Shi; Mingbo Sun
Source:
Biologicals 2022 v.75 pp. 3-11
ISSN:
1045-1056
Subject:
antibody formation,   etc   ; antigens; immunogenicity; seroconversion; serotypes; vaccination; vaccines;     Show all 7 Subjects
Abstract:
... Reducing the amount of antigen is an important strategy to resolve the present shortage of IPV supply for global polio eradication. In the study, we compared the immunogenicity of adjuvanted and non-adjuvanted fractional-dose of IPV made from Sabin strains (sIPV) by intradermal (ID) administration versus the full-dose of sIPV by intramuscular (IM) administration in rats by comparing seroconversion ...
DOI:
10.1016/j.biologicals.2022.01.005

3. Antimicrobial peptides: Promising alternatives over conventional capture ligands for biosensor-based detection of pathogenic bacteria

Author:
M. Amirul Islam; Ahasanul Karim; Baranitharan Ethiraj; Topu Raihan; Abudukeremu Kadier
Source:
Biotechnology advances 2022 v.55 pp. 107901
ISSN:
0734-9750
Subject:
antibody formation,   etc   ; antimicrobial peptides; biosensors; biotechnology; electrochemistry; ligands; mammals;     Show all 7 Subjects
Abstract:
... The detection of pathogenic bacteria using biosensing techniques could be a potential alternative to traditional culture based methods. However, the low specificity and sensitivity of conventional biosensors, critically related to the choice of bio-recognition elements, limit their practical applicability. Mammalian antibodies have been widely investigated as biorecognition ligands due to high spe ...
DOI:
10.1016/j.biotechadv.2021.107901

4. Receptor-enhanced immunogenicity of anthrax protective antigen is primarily mediated by capillary morphogenesis Protein-2

Author:
Anita Verma; Mahtab Moayeri; Christopher P. Mocca; Danielle O'Mard; Qian Ma; Stephen H. Leppla; Drusilla L. Burns
Source:
Vaccine 2022 v.40 no.32 pp. 4318-4321
ISSN:
0264-410X
Subject:
antibody formation,   etc   ; anthrax; anthrax toxin; immunogenicity; morphogenesis; neoplasms; vaccines;     Show all 7 Subjects
Abstract:
... Anthrax protective antigen (PA), the receptor-binding component of anthrax toxin, elicits toxin-neutralizing antibodies which provide protection against anthrax disease. PA binds to two mammalian receptors, capillary morphogenesis protein-2 (CMG2) and tumor endothelial marker-8 (TEM8). We previously observed that binding of PA to its receptors plays a role in eliciting a strong toxin-neutralizing ...
DOI:
10.1016/j.vaccine.2022.06.023

5. Antigenicity and immunogenicity of HA2 and M2e influenza virus antigens conjugated to norovirus-like, VP1 capsid-based particles by the SpyTag/SpyCatcher technology

Author:
Suvi Heinimäki; Vili Lampinen; Kirsi Tamminen; Minna M. Hankaniemi; Maria Malm; Vesa P. Hytönen; Vesna Blazevic
Source:
Virology 2022 v.566 pp. 89-97
ISSN:
0042-6822
Subject:
antibody formation,   etc   ; Orthomyxoviridae; glycoproteins; hemagglutinins; immunogenicity; influenza; peptides; vaccines; virology;     Show all 9 Subjects
Abstract:
... Virus-like particles (VLPs) modified through different molecular technologies are employed as delivery vehicles or platforms for heterologous antigen display. We have recently created a norovirus (NoV) VLP platform, where two influenza antigens, the extracellular domain of matrix protein M2 (M2e) or the stem domain of the major envelope glycoprotein hemagglutinin (HA2) are displayed on the surface ...
DOI:
10.1016/j.virol.2021.12.001

6. Exploring the limits of conventional small-scale CHO fed-batch for accelerated on demand monoclonal antibody production

Author:
Amélie Mahé; Alexandra Martiné; Séverine Fagète; Pierre-Alain Girod
Source:
Bioprocess and biosystems engineering 2022 v.45 no.2 pp. 297-307
ISSN:
1615-7591
Subject:
antibody formation,   etc   ; biomass; bioprocessing; monoclonal antibodies; product quality; supply balance; therapeutics;     Show all 7 Subjects
Abstract:
... In the field of therapeutic antibody production, diversification of fed-batch strategies is flourishing in response to the market demand. All manufacturing approaches tend to follow the generally accepted dogma of increasing titer since it directly increases manufacturing output. While titer is influenced by the biomass (expressed as IVCD), the culture time and the cell-specific productivity (qP), ...
DOI:
10.1007/s00449-021-02657-w

7. Proteomic assessment of humoral immune responses in smallpox vaccine recipients

Author:
Richard B. Kennedy; Inna G. Ovsyannikova; Iana H. Haralambieva; Diane E. Grill; Gregory A. Poland
Source:
Vaccine 2022 v.40 no.5 pp. 789-797
ISSN:
0264-410X
Subject:
antibody formation,   etc   ; animals; globulins; microarray technology; monitoring; proteome; proteomics; smallpox; vaccines;     Show all 9 Subjects
Abstract:
... The availability of effective smallpox vaccines was a critical element of the successful eradication of smallpox in 1980. Antibody responses play a primary role in protective immunity and neutralizing antibody is an established correlate of protection against smallpox. In this study we used a poxvirus proteome array to assess the antibody response to individual viral proteins in a cohort of 1,037 ...
DOI:
10.1016/j.vaccine.2021.12.033

8. Increase recombinant antibody yields through optimizing vector design and production process in CHO cells

Author:
Yongxiao Yang; Zhengmei Li; Qin Li; Kai Ma; Yan Lin; Huigen Feng; Tianyun Wang
Source:
Applied microbiology and biotechnology 2022 v.106 no.13-16 pp. 4963-4975
ISSN:
0175-7598
Subject:
antibody formation,   etc   ; Cricetulus griseus; biotechnology; genetic vectors; microbiology; necrosis; neoplasms; recombinant antibodies;     Show all 8 Subjects
Abstract:
... Chinese hamster ovary (CHO) cells are the most commonly used host cells for the production of recombinant monoclonal antibodies (mAbs) due to their several advantages. Although the yields of recombinant mAbs can be greatly increased by some strategies, such as medium formulation, culture conditions, and cell engineering, most studies focused on either upstream design or downstream processes. In th ...
DOI:
10.1007/s00253-022-12051-5

9. The Neutralizing Antibody Responses of Individuals That Spontaneously Resolve Hepatitis C Virus Infection

Author:
Vanessa M. Cowton; James I. Dunlop; Sarah J. Cole; Rachael E. Swann; Arvind H. Patel
Source:
Viruses 2022 v.14 no.7 pp. -
ISSN:
1999-4915
Subject:
antibody formation,   etc   ; Hepatitis C virus; hepatitis C; mutagenesis; vaccine development; vaccines; viruses;     Show all 7 Subjects
Abstract:
... Hepatitis C virus (HCV) infection is a major global health problem. In the majority of cases the virus is not cleared by the host immune response and progresses to chronic infection. Studies of the neutralizing antibody responses in individuals that naturally clear infection are limited. Understanding what constitutes a successful antibody response versus one that has ‘failed’ and resulted in chro ...
DOI:
10.3390/v14071391

10. Clinical relevance of increased antibody titres in older adults upon vaccination with squalene-adjuvanted versus non-adjuvanted influenza vaccines

Author:
Walter E.P. Beyer; Abraham M. Palache; Mimoun Boulfich; Albert D.M.E. Osterhaus
Source:
Vaccine 2022 v.40 no.23 pp. 3098-3102
ISSN:
0264-410X
Subject:
antibody formation,   etc   ; antibodies; blood serum; clinical trials; influenza; influenza vaccines; meta-analysis; vaccination;     Show all 8 Subjects
Abstract:
... In older adults, the serum antibody response to inactivated influenza vaccine (IIV) is often lower than in adolescents and non-elderly adults which may translate into suboptimal protection against influenza. To counteract this expression of immunosenescence, the use of adjuvanted IIV formulations has been explored. Four recent studies (three meta-analyses and one clinical trial) found an antibody ...
DOI:
10.1016/j.vaccine.2022.04.049

11. Non-glycosylated SARS-CoV-2 RBD elicited a robust neutralizing antibody response in mice

Author:
Qian Ke; Peng Sun; Tiantian Wang; Taotao Mi; Huifang Xu; Jun Wu; Bo Liu
Source:
Journal of immunological methods 2022 v.506 pp. 113279
ISSN:
0022-1759
Subject:
antibody formation,   etc   ; Escherichia coli; Severe acute respiratory syndrome coronavirus 2; glycosylation; oligodeoxyribonucleotides; vaccines;     Show all 6 Subjects
Abstract:
... The glycosylated receptor-binding domain (glycoRBD) of SARS-CoV-2 can induce protective neutralizing antibodies to function as a vaccine. However, it is unclear whether vaccines using non-glycosylated RBD (non-glycoRBD) can induce protective immunity. Here, we report the efficacy of a SARS-CoV-2 non-glycoRBD vaccine produced by prokaryotic system in mice. The recombinant non-glycoRBD protein was o ...
DOI:
10.1016/j.jim.2022.113279

12. Highly efficient and precise two-step cell selection method for tetramethylenedisulfotetramine-specific monoclonal antibody production

Author:
Yuan Li; Ling Yang; Wenbo Yu; Xuezhi Yu; Kai Wen; Bing Shao; Jiefang Sun; Jianzhong Shen; Zhanhui Wang
Source:
Journal of hazardous materials 2022 v.424 pp. 127689
ISSN:
0304-3894
Subject:
antibody formation,   etc   ; antibiotics; diagnostic techniques; flow cytometry; haptens; hybridomas; monoclonal antibodies; neurotoxicity; rodenticides;     Show all 9 Subjects
Abstract:
... Monoclonal antibodies (mAbs) are useful biological tools for research, diagnostics, and pharmaceuticals. Here, we proposed a new mAb discovery platform named the two-step cell selection method (TCSM) for mAbs production of some small molecule haptens as antibiotic, toxins, and pesticides. The first step was performed by a fluorescence-activated cell sorter to enrich the hapten-specific B cells, th ...
DOI:
10.1016/j.jhazmat.2021.127689

13. Polycationic HA/CpG Nanoparticles Induce Cross-Protective Influenza Immunity in Mice

Author:
Chunhong Dong; Ye Wang; Wandi Zhu; Yao Ma; Joo Kim; Lai Wei; Gilbert X. Gonzalez; Bao-Zhong Wang
Source:
ACS applied materials & interfaces 2022 v.14 no.5 pp. 6331-6342
ISSN:
1944-8252
Subject:
antibody formation,   etc   ; antibodies; cross immunity; cytotoxicity; glycoproteins; immunogenicity; influenza; influenza vaccines; neutralization; polyethyleneimine; vaccination;     Show all 11 Subjects
Abstract:
... The intranasal (i.n.) route is an ideal vaccination approach for infectious respiratory diseases like influenza. Polycationic polyethylenimine (PEI) could form nanoscale complexes with negatively charged viral glycoproteins. Here we fabricated PEI-hemagglutinin (HA) and PEI-HA/CpG nanoparticles and investigated their immune responses and protective efficacies with an i.n. vaccination regimen in mi ...
DOI:
10.1021/acsami.1c19192

14. Analysis of immune response induction mechanisms implicating the dose-sparing effect of transcutaneous immunization using a self-dissolving microneedle patch

Author:
Sayami Ito; Sachiko Hirobe; Ryo Yamashita; Arisa Sugiyama; Honoka Takeuchi; Ryosuke Eguchi; Junya Yoshida; Takayoshi Oyamada; Masashi Tachibana; Naoki Okada
Source:
Vaccine 2022 v.40 no.6 pp. 862-872
ISSN:
0264-410X
Subject:
antibody formation,   etc   ; B-lymphocytes; cell differentiation; cytokines; gene expression; lymph; transcription factors; vaccination; vaccines;     Show all 9 Subjects
Abstract:
... Transcutaneous immunization (TCI) is an effective vaccination method that is easier and less painful than the conventional injectable vaccination method. We previously developed self-dissolving microneedle patches (sdMN) and demonstrated that this TCI method has a high vaccination efficacy in mice and humans. To elucidate the mechanism of immune response induction, which is the basis for the effic ...
DOI:
10.1016/j.vaccine.2021.12.055

15. Canine antibody response against Dirofilaria repens in natural occult and microfilaremic infections

Author:
Magdalena E. Wysmołek; Maciej Klockiewicz; Ewa Długosz; Marcin Wiśniewski
Source:
Comparative immunology, microbiology, and infectious diseases 2022 v.86 pp. 101818
ISSN:
0147-9571
Subject:
antibody formation,   etc   ; Dirofilaria repens; blood serum; dirofilariasis; dogs; etiological agents; immunoglobulin G; microbiology; Europe;     Show all 9 Subjects
Abstract:
... Dirofilaria repens is the causative agent of the zoonotic canine skin condition called subcutaneous dirofilariosis. Despite being endemic throughout much of Europe, little is known about host humoral responses to D. repens. To address this, we analyzed serum immunoglobulin isotypes recognizing D. repens somatic antigens (DrSA) in naturally infected dogs. Titers of anti-DrSA IgG and IgE, but not Ig ...
DOI:
10.1016/j.cimid.2022.101818

16. IL- 4 plays an essential role in DnaJ-ΔA146Ply-mediated immunoprotection against Streptococcus pneumoniae in mice

Author:
Yi Hu; Lian Li; Wenchun Xu; Kaifeng Wu; Jiangming Xiao; Yang Peng; Yusi Liu; Yibing Yin; Xuemei Zhang
Source:
Molecular immunology 2022 v.143 pp. 105-113
ISSN:
0161-5890
Subject:
antibody formation,   etc   ; Streptococcus pneumoniae; antibodies; blood serum; cell-mediated immunity; interleukin-4; neutrophils; phagocytosis; splenocytes;     Show all 9 Subjects
Abstract:
... The fusion protein DnaJ-ΔA146Ply is protective against pneumococcal infections in mice. However, we found that immunized IL-4⁻/⁻ mice showed significant lower survival rates and higher bacterial loads than did wild-type (WT) mice after being challenged. We explored the role of IL-4 in the protective immunity conferred by DnaJ-ΔA146Ply. Our results showed that there were no significant differences ...
DOI:
10.1016/j.molimm.2022.01.010

17. Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows

Author:
C.M. Camussone; I.G. Reidel; A.I. Molineri; J. Cicotello; C. Miotti; G.A. Suarez Archilla; C.C. Curti; C. Veaute; L.F. Calvinho
Source:
Research in veterinary science 2022 v.145 pp. 177-187
ISSN:
0034-5288
Subject:
antibody formation,   etc   ; disease incidence; hemolysis; immunization; immunoglobulin G; milk; oligodeoxyribonucleotides; research; subunit vaccines; veterinary medicine;     Show all 10 Subjects
Abstract:
... The aims of the present study were to evaluate the ability of a subunit vaccine composed of recombinant molecules of α-toxin, β-toxin, FnBPA and ClfA, formulated with cationic liposomes and CpG-ODN, to confer protection against natural S. aureus intramammary infection (IMI) and to assess the antibody response against the vaccine components. A stringent criterion based on molecular identification o ...
DOI:
10.1016/j.rvsc.2022.02.014

18. Evaluation of monophosphoryl lipid A as an adjuvanted for inactivated chikungunya virus

Author:
Mrunal Gosavi; Harshad P. Patil
Source:
Vaccine 2022 v.40 no.34 pp. 5060-5068
ISSN:
0264-410X
Subject:
antibody formation,   etc   ; Chikungunya virus; adjuvants; antigens; immunization; immunogenicity; interleukin-4; ligands; lipid A; splenocytes; vaccines;     Show all 11 Subjects
Abstract:
... Currently there is no clinically approved chikungunya virus (CHIKV) vaccine for immunization. Though definite need is felt, long disappearance of CHIKV has been a concern. Inactivated CHIKV (I-CHIKV) is an attractive antigen to develop effective vaccines within a short period of time. However, highly purified inactivated CHIKV do not contain necessary triggers for induction of robust antibody resp ...
DOI:
10.1016/j.vaccine.2022.07.018

19. Induction of Cross-Reacting Antibodies Against the COVID-19 by BCG Vaccination in the Mouse Model

Author:
Nadia Rahali; Chokri Bahloul
Source:
Current microbiology 2022 v.79 no.9 pp. 275
ISSN:
0343-8651
Subject:
antibody formation,   etc   ; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; mice; microbiology; rabies; tuberculosis; vaccination;     Show all 8 Subjects
Abstract:
... It was reported that tuberculosis and BCG vaccination are potential tools for reducing the burden of COVID-19, mainly through the non-specific trained immunity. We have investigated whether BCG vaccination is able to induce cross-reacting antibodies against the SARS-CoV-2. We have tested the induced humoral immune responses against the SARS-CoV-2 Spike in the mouse model, after either BCG or rabie ...
DOI:
10.1007/s00284-022-02971-w

20. Insight into Trichinella britovi Infection in Pigs: Effect of Various Infectious Doses on Larvae Density and Spatial Larvae Distribution in Carcasses and Comparison of the Detection of Anti-T. britovi IgG of Three Different Commercial ELISA Tests and Immunoblot Assay

Author:
Michał Gondek; Sylwia Grzelak; Renata Pyz-Łukasik; Przemysław Knysz; Monika Ziomek; Justyna Bień-Kalinowska
Source:
Pathogens 2022 v.11 no.7 pp. -
ISSN:
2076-0817
Subject:
antibody formation,   etc   ; Trichinella britovi; diaphragm; humoral immunity; hygiene; meat; parasites; pathogenicity; swine; swine production; veterinary clinics;     Show all 11 Subjects
Abstract:
... There is limited information available on the Trichinella britovi (T. britovi) muscle larvae (ML) distribution in pig muscle and the humoral immune response of pigs infected with moderate doses of this parasite; therefore, this study investigated the infectivity of a Polish strain of T. britovi for pigs, the antibody response of this host to various doses of T. britovi, and the efficiency of three ...
DOI:
10.3390/pathogens11070735