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1. A novel flower-shaped Ag@ZIF-67 chemiluminescence sensor for sensitive detection of CEA

Author:
Yanan Hou; Jingdao Wang; Shantian Liu; Yuanling Sun; Yuxue Dai; Chuannan Luo; Xueying Wang
Source:
Talanta 2023 v.253 pp. 123938
ISSN:
0039-9140
Subject:
antigens,   etc   ; aptasensors; blood serum; carbon nanotubes; chemiluminescence; detection limit; humans; magnetic fields; oligonucleotides;     Show all 9 Subjects
Abstract:
... In this work, a chemiluminescence (CL) aptasensor for sensitive carcinoembryonic antigen (CEA) detection was constructed based on the CL system of luminol–H₂O₂–NaOH. Magnetic carbon nanotubes (MCNTs), as the base material, was modified with CEA-aptamer and DNA1, and was combined with the novel flower-shaped Ag@ZIF-67 of modified with DNA2 through the principle of base complementary pairing. CEA co ...
DOI:
10.1016/j.talanta.2022.123938

2. Rapid electrochemical detection of Mycobacterium tuberculosis in sputum by measuring Ag85 activity with disposable carbon sensors

Author:
Elodie Barbier; Théo Fouchet; Alain Hartmann; Emmanuelle Cambau; Faiza Mougari; Clément Dubois; Maurice Lubetzki; Murielle Rochelet
Source:
Talanta 2023 v.253 pp. 123927
ISSN:
0039-9140
Subject:
antigens,   etc   ; Mycobacterium tuberculosis; biomarkers; carbon; decontamination; electrochemistry; enzyme activity; fatty acids; microscopy; oxidation; tuberculosis; voltammetry;     Show all 12 Subjects
Abstract:
... An electrochemical assay for the detection of the enzymatic activity of the antigen 85 (Ag85) tuberculosis (TB) biomarker was developed and evaluated for the qualitative detection of Mycobacterium tuberculosis in decontaminated sputum. For this purpose, the electroactive properties of both synthetic p-aminophenyl-6-O-octanoyl-3-d-glucopyranoside (p-APOG) substrate and p-aminophenyl-6-3-d-glucopyra ...
DOI:
10.1016/j.talanta.2022.123927

3. A sensitive electrochemiluminescence immunosensor for the detection of CA15-3 based on CeO2/Pt/rGO as a novel co-reaction accelerator

Author:
Zhenbin Lin; Shaoqin Zheng; Jinbiao Xie; Runzhi Zhou; Yaowen Chen; Wenhua Gao
Source:
Talanta 2023 v.253 pp. 123912
ISSN:
0039-9140
Subject:
antigens,   etc   ; biomedical research; breast neoplasms; detection limit; electrical conductivity; electrochemiluminescence; graphene oxide; immunosensors; nanoparticles; silica; surface area;     Show all 11 Subjects
Abstract:
... In this work, we successfully constructed a label-free electrochemiluminescence (ECL) immunosensor for the detection of breast cancer marker antigen (CA15-3). In particular, 3,4,9,10-perylenetetracarboxylic acid (PTCA) is cleverly attached to the surface of silica spheres as a luminophore (NH₂-SiO₂-PTCA), which greatly alleviates the disadvantage of PTCA anti-induced aggregated luminescence and im ...
DOI:
10.1016/j.talanta.2022.123912

4. Production of high affinity monoclonal antibody and development of indirect competitive chemiluminescence enzyme immunoassay for gentamicin residue in animal tissues

Author:
Peng Dai; Yan Zhang; Yanping Hong; Jianhua Xiong; Huaying Du; Luying Duan; Dan Wang; Yubo Wang; Weijie Deng; Bruce D. Hammock; Wuying Yang
Source:
Food chemistry 2023 v.400 pp. 134067
ISSN:
0308-8146
Subject:
antigens,   etc   ; bovine serum albumin; chemiluminescence; cross reaction; detection limit; enzyme immunoassays; fish; food chemistry; gentamicin; hybridomas; monoclonal antibodies; pork;     Show all 12 Subjects
Abstract:
... To determine gentamicin residues in animal tissues, a monoclonal antibody (Mab) was produced and a sensitive indirect competitive chemiluminescent enzyme immunoassay (icCLEIA) was developed. At first, gentamicin was conjugated with bovine serum albumin as immunogens which were used to immunize BALB/c mice. Then, an anti-gentamicin Mab was prepared by hybridoma technology. Finally, a sensitive icCL ...
DOI:
10.1016/j.foodchem.2022.134067

5. Electrochemical paper-based antigen sensing platform using plant-derived monoclonal antibody for detecting SARS-CoV-2

Author:
Jutamas Jaewjaroenwattana; Waranyoo Phoolcharoen; Ekawat Pasomsub; Prinjaporn Teengam; Orawon Chailapakul
Source:
Talanta 2023 v.251 pp. 123783
ISSN:
0039-9140
Subject:
antigens,   etc   ; COVID-19 infection; Nicotiana benthamiana; Severe acute respiratory syndrome coronavirus 2; cellulose; cost effectiveness; detection limit; electrochemistry; electrodes; graphene; immunosensors; monoclonal antibodies; nanocrystals; saliva; voltammetry;     Show all 15 Subjects
Abstract:
... The current approaches of diagnostic platforms for detecting SARS-CoV-2 infections mostly relied on adapting the existing technology. In this work, a simple and low-cost electrochemical sensing platform for detecting SAR-CoV-2 antigen was established. The proposed sensor combined the innovative disposable paper-based immunosensor and cost-effective plant-based anti-SARS-CoV-2 monoclonal antibody C ...
DOI:
10.1016/j.talanta.2022.123783

6. Measuring the sero-prevalence of Leishmania donovani induced cutaneous leishmaniasis: A method comparison study

Author:
Bhagya Deepachandi; Sarfaraz A. Ejazi; Anirban Bhattacharyya; Nahid Ali; Preethi Soysa; Yamuna Siriwardana
Source:
Parasitology international 2023 v.92 pp. 102660
ISSN:
1383-5769
Subject:
antigens,   etc   ; Leishmania donovani; agglutination tests; antibodies; cost analysis; cutaneous leishmaniasis; diagnostic techniques; enzyme-linked immunosorbent assay; parasites; parasitology; patients; point-of-care systems; seroprevalence; Sri Lanka;     Show all 14 Subjects
Abstract:
... An in-house enzyme-linked immunosorbent assay (ELISA) based on crude antigen of Leishmania reported a high sero-prevalence (82.0%) in Leishmania donovani induced cutaneous leishmaniasis (CL) in Sri Lanka. ELISA was further compared with established serological tools to identify a suitable point of care diagnostic tool. Sero-prevalence of 100 CL samples were analyzed using in-house ELISA, Indian di ...
DOI:
10.1016/j.parint.2022.102660

7. Lowering the viscosity of a high-concentration antibody solution by protein–polyelectrolyte complex

Author:
Keisuke Tsumura; WeiLin Hsu; Masahiro Mimura; Aiko Horiuchi; Kentaro Shiraki
Source:
Journal of bioscience and bioengineering 2022 v.133 no.1 pp. 17-24
ISSN:
1389-1723
Subject:
antigens,   etc   ; monoclonal antibodies; phosphates; viscosity;     Show all 4 Subjects
Abstract:
... High-concentration and low-viscosity antibody formulations are necessary when administering these solutions subcutaneously (SC) due to limitations on injection volume. Here we show a method to decrease the viscosity of monoclonal antibody solution by protein–polyelectrolyte complex (PPC) with poly-l-glutamic acid (polyE). The viscosity of omalizumab solutions was 90 cP at the concentration of 150  ...
DOI:
10.1016/j.jbiosc.2021.09.011

8. Diagnosis of osteoarticular tuberculosis by immuno‐PCR assay based on mycobacterial antigen 85 complex detection

Author:
A. Khan; R. Singh; S. Sharma; V. Singh; A. Sheoran; A. Soni; V. Dhull; P.S. Gill; A. Yadav; D. Chaudhary; M.C. Gupta; P.K. Mehta
Source:
Letters in applied microbiology 2022 v.74 no.1 pp. 17-26
ISSN:
0266-8254
Subject:
antigens,   etc   ; analytical kits; microbiology; tuberculosis;     Show all 4 Subjects
Abstract:
... Diagnosis of osteoarticular tuberculosis (OATB) exhibits serious challenges owing to paucibacillary nature of specimens and localization of disease at sites that are difficult to access. We recently developed indirect immuno‐PCR (I‐PCR) and real‐time I‐PCR (RT‐I‐PCR) assays for the detection of mycobacterial antigen 85 complex (Ag85) in OATB patients. Detection limits for the purified Ag85 protein ...
DOI:
10.1111/lam.13567

9. Combining ionic diode, resistive pulse and membrane for detection and separation of anti-CD44 antibody

Author:
Imad Abrao-Nemeir; Oumaima Zaki; Nathan Meyer; Mathilde Lepoitevin; Joan Torrent; Jean-Marc Janot; Sebastien Balme
Source:
Journal of membrane science 2022 v.649 no. pp. 120391
ISSN:
0376-7388
Subject:
antigens,   etc   ; antibodies; diodes; nanopores; polymers; therapeutics;     Show all 6 Subjects
Abstract:
... The need for antibody proteins is growing for both diagnosis and therapy. In this work, we combine two strategies based on single nanopore sensing and multipore membrane to separate, detect and identify antibodies. This proof of concept was done for the anti-CD44. In a first step, a single nanopore with a bullet shape were designed using track-etching of polymer film and functionalized, with a PEG ...
DOI:
10.1016/j.memsci.2022.120391

10. NK Cells in Protection from HIV Infection

Author:
Nicole F. Bernard; Khlood Alsulami; Erik Pavey; Franck P. Dupuy
Source:
Viruses 2022 v.14 no.6 pp. -
ISSN:
1999-4915
Subject:
antigens,   etc   ; HIV infections; antiviral properties; ligands;     Show all 4 Subjects
Abstract:
... Some people, known as HIV-exposed seronegative (HESN) individuals, remain uninfected despite high levels of exposure to HIV. Understanding the mechanisms underlying their apparent resistance to HIV infection may inform strategies designed to protect against HIV infection. Natural Killer (NK) cells are innate immune cells whose activation state depends on the integration of activating and inhibitor ...
DOI:
10.3390/v14061143

11. Selective retrieval of antibody-secreting hybridomas in cell arrays based on the dielectrophoresis

Author:
Misaki Hata; Masato Suzuki; Tomoyuki Yasukawa
Source:
Biosensors & bioelectronics 2022 v.209 pp. 114250
ISSN:
0956-5663
Subject:
antigens,   etc   ; antibodies; biosensors; dielectrophoresis; hybridomas; secretion;     Show all 6 Subjects
Abstract:
... A cascade of the formation of cell arrays, the discrimination of cells secreting specific molecules, and the selective retrieval of cells has been developed to harvest antibody-secreting hybridomas in heterogeneous cell populations simply and rapidly. The microwell array device consisted of three-dimensional microband electrodes by assembling both upper and lower substrates perpendicularly. Arrays ...
DOI:
10.1016/j.bios.2022.114250

12. Tuning the ignition of CAR: optimizing the affinity of scFv to improve CAR-T therapy

Author:
Yanting Duan; Ruoqi Chen; Yanjie Huang; Xianhui Meng; Jiangqing Chen; Chan Liao; Yongmin Tang; Chun Zhou; Xiaofei Gao; Jie Sun
Source:
Cellular and molecular life sciences 2022 v.79 no.1 pp. 14
ISSN:
1420-682X
Subject:
antigens,   etc   ; mice; synthetic biology; therapeutics; toxicity;     Show all 5 Subjects
Abstract:
... How single-chain variable fragments (scFvs) affect the functions of chimeric antigen receptors (CARs) has not been well studied. Here, the components of CAR with an emphasis on scFv were described, and then several methods to measure scFv affinity were discussed. Next, scFv optimization studies for CD19, CD38, HER2, GD2 or EGFR were overviewed, showing that tuning the affinity of scFv could allevi ...
DOI:
10.1007/s00018-021-04089-x

13. An improved method for rapid identification of hook effect samples in HBsAg quantitative assay

Author:
Mingxin Chen; Yu Tian; Dai Zhang; Weihong Ren; Wei Wang
Source:
Journal of virological methods 2022 v.309 pp. 114606
ISSN:
0166-0934
Subject:
hepatitis B antigens,   etc   ; chemiluminescence; hepatitis B;     Show all 3 Subjects
Abstract:
... Quantitative hepatitis B surface antigen assay is widely used for diagnosis of hepatitis B virus infection; however, specimens with high levels of the antigen can cause false-negative results (Hook effect), which needs to be resolved. The hook effect samples and non-hook effect samples were detected on the LiCA® 500 instrument using three methods, viz., 1, 2, and 3. Method 1, the currently used pr ...
DOI:
10.1016/j.jviromet.2022.114606

14. Protein abundance of the cytokine receptor γc controls the thymic generation of innate-like T cells

Author:
Joo-Young Park; Hee Yeun Won; Devon T. DiPalma; Changwan Hong; Jung-Hyun Park
Source:
Cellular and molecular life sciences 2022 v.79 no.1 pp. 17
ISSN:
1420-682X
Subject:
antigens,   etc   ; cell differentiation; cytokine receptors; immunomodulation; thymocytes;     Show all 5 Subjects
Abstract:
... Innate-like T (iT) cells comprise a population of immunoregulatory T cells whose effector function is imposed during their development in the thymus to provide protective immunity prior to antigen encounter. The molecular mechanism that drives the generation of iT cells remains unclear. Here, we report that the cytokine receptor γc plays a previously unappreciated role for thymic iT cells by contr ...
DOI:
10.1007/s00018-021-04067-3

15. A novel and effective natural product-based immunodetection tool for TNT-like compounds

Author:
Mladen Simonovic; Sanja Ostojic; Darko Micic; Marjetka Bisercic-Savic; Thorsten Mix; Miodrag Glumac; Boris Pejin
Source:
Natural product research 2022 v.36 no.3 pp. 857-861
ISSN:
1478-6427
Subject:
antigens,   etc   ; enzyme-linked immunosorbent assay; models; research;     Show all 4 Subjects
Abstract:
... This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a β-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-β-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (β- ...
DOI:
10.1080/14786419.2020.1806269

16. Product-safety considerations in allogeneic chimeric antigen-receptor T-cell process flows

Author:
Bryan Dransart; Houman Dehghani; Alison Moore
Source:
Current opinion in biotechnology 2022 v.78 pp. 102797
ISSN:
0958-1669
Subject:
antigens,   etc   ; T-lymphocytes; biotechnology; electroporation; product safety;     Show all 5 Subjects
Abstract:
... Allogeneic chimeric antigen-receptor T-cell process flows comprise large-scale primary cultures incorporating traditional biologics processing steps such as cell expansion, with novel processing steps such as electroporation. In addition, these process flows begin with donor-derived peripheral blood mononuclear cells and generate single batches of product, which may be used to treat hundreds of pa ...
DOI:
10.1016/j.copbio.2022.102797

17. Rhamnose-based glycomimetic for recruitment of endogenous anti-rhamnose antibodies

Author:
Nives Hribernik; Fabrizio Chiodo; Roland J. Pieters; Anna Bernardi
Source:
Tetrahedron letters 2022 v.99 pp. 153843
ISSN:
0040-4039
Subject:
antigens,   etc   ; antibodies; immune system; neoplasms; rhamnose; therapeutics;     Show all 6 Subjects
Abstract:
... Recruitment of natural antibodies towards tumour cells for their elimination by the immune system could be a highly specific and efficacious therapeutic strategy. While natural l-rhamnose has already been explored as a suitable antigen for antibody recruitment, we here report the first rhamnose-based glycomimetic to be used for such purpose. The glycomimetic is designed to be more hydrolytically a ...
DOI:
10.1016/j.tetlet.2022.153843

18. Glycoconjugate content quantification to assess vaccine potency: A simplified approach

Author:
Francesca Rech; Riccardo De Ricco; Sara Giannini; Luca Nompari; Marilena Paludi; Stefania Berti; Chiara Parlati; Simona Cianetti; Francesco Berti
Source:
Biologicals 2022 v.76 pp. 10-14
ISSN:
1045-1056
Subject:
antigens,   etc   ; phosphates; polysaccharides; product quality; serotypes; vaccines;     Show all 6 Subjects
Abstract:
... Several glycoconjugate vaccines have been licensed or are currently in clinical development to prevent bacterial infections. Here we report the development of a single analytical assay to quantify the conjugated saccharide content, as alternative to two separated total and free (unconjugated) saccharide assays used so far, for a quadrivalent conjugate vaccine containing meningococcal serogroup A p ...
DOI:
10.1016/j.biologicals.2022.02.004

19. Lewis b antigen is a common ligand for genogroup I norovirus strains

Author:
Yuichi Someya
Source:
FEBS Open Bio 2022 v.12 no.9 pp. 1688-1695
ISSN:
2211-5463
Subject:
antigens,   etc   ; Norovirus; gastroenteritis; genotype; insects; ligands; pathogenesis;     Show all 7 Subjects
Abstract:
... Noroviruses are major causative agents of nonbacterial acute gastroenteritis in humans. Ten genogroups of noroviruses have been identified to date, among which genogroup I (GI) and genogroup II (GII) noroviruses are major pathogens for humans. GI and GII noroviruses are further classified into nine and 27 genotypes, respectively. Noroviruses are well known to bind to histo‐blood group antigens (HB ...
DOI:
10.1002/2211-5463.13455

20. Mannose in vaccine delivery

Author:
Ummey Jannatun Nahar; Istvan Toth; Mariusz Skwarczynski
Source:
Journal of controlled release 2022 v.351 pp. 284-300
ISSN:
0168-3659
Subject:
antigens,   etc   ; immune system; ligands; mannose; mannosylation; vaccines;     Show all 6 Subjects
Abstract:
... Adjuvants and vaccine delivery systems are used widely to improve the efficacy of vaccines. Their primary roles are to protect antigen from degradation and allow its delivery and uptake by antigen presenting cells (APCs). Carbohydrates, including various structures/forms of mannose, have been broadly utilized to target carbohydrate binding receptors on APCs. This review summarizes basic functions ...
DOI:
10.1016/j.jconrel.2022.09.038