U.S. flag

An official website of the United States government

PubAg

Main content area

You searched for: Subject "assays" Remove constraint Subject: "assays"
Start Over

Search

25,710  Search Results

« Previous | 1 - 20 of 25,710 |
Number of results to display per page

Search Results

1. Rutin‐protected BisGMA‐induced cytotoxicity, genotoxicity, and apoptosis in macrophages through the reduction of the mitochondrial apoptotic pathway and induction of antioxidant enzymes

Author:
Huang, Fu‐Mei; Chang, Yu‐Chao; Su, Chun‐Hung; Wu, Sheng‐Wen; Lee, Shiuan‐Shinn; Lee, Min‐Wei; Yeh, Kun‐Lin; Chiang, Chen‐Yu; Tu, Dom‐Gene; Lu, Yin‐Che; Kuan, Yu‐Hsiang
Source:
Environmental toxicology 2021 v.36 no.1 pp. 45-54
ISSN:
1520-4081
Subject:
assays; comet assay,   etc   ; antioxidant enzymes; apoptosis; caspase-3; caspase-8; cytotoxicity; ecotoxicology; genotoxicity; lead; macrophages; mitochondria; phosphatidylserines; polymers; pretreatment; reactive oxygen species; resins; rutin;     Show all 18 Subjects
Abstract:
... Bisphenol‐A‐glycidyldimethacrylate (BisGMA) is a resin monomer frequently used in dentin restorative treatments. The leakage of BisGMA monomer from BisGMA‐based polymeric resins can lead to cytotoxicity in macrophages. Rutin has various beneficial bioeffects, including antioxidation and antiinflammation. In this study, we found that pretreatment of RAW264.7 macrophages with rutin‐inhibited cytotox ...
DOI:
10.1002/tox.23009

2. Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon

Author:
Ramos, Patrícia Karla Santos; Gonçalves, Lucas Pantoja; Alves, Ana Camila Oliveira; Casseb, Samir Mansour; Lima, Luciana Vieira do Rêgo; Campos, Marliane Batista; Vasconcelos dos Santos, Thiago; Silveira, Fernando Tobias
Source:
Parasitology international 2021 v.81 pp. 102273
ISSN:
1383-5769
Subject:
assays,   etc   ; DNA; Leishmania; area; evolution; extraction; humans; infection; monitoring; parasitology; sampling; sediments; urine; visceral leishmaniasis; Amazonia;     Show all 15 Subjects
Abstract:
... The clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon has been defined using DTH/IFAT-IgG immune assays and the clinical statuses of infected individuals, revealing five profiles: three asymptomatic [Asymptomatic Infection (AI), Subclinical Resistant Infection (SRI), and Indeterminate Initial Infection (III)], and two symptomatic profiles ...
DOI:
10.1016/j.parint.2020.102273

3. A smartphone-assisted portable biosensor using laccase-mineral hybrid microflowers for colorimetric determination of epinephrine

Author:
Zhang, Miaorong; Zhang, Yan; Yang, Chuankai; Ma, Chunyun; Tang, Jianguo
Source:
Talanta 2021 v.224 pp. 121840
ISSN:
0039-9140
Subject:
assays,   etc   ; biomineralization; biosensors; colorimetry; detection limit; epinephrine; hybrids; laccase; nanomaterials; protective effect;     Show all 10 Subjects
Abstract:
... A novel smartphone-assisted portable biosensor based on laccase-mineral hybrid microflowers (La-HMFs) was applied for real-time, accurate and reliable quantification of epinephrine (EP). La-HMFs with flower-like hierarchical nanostructure was synthesized via biomineralization using Cu₃(PO₄)₂⋅3H₂O as the mineral. Characterization results revealed laccase molecules as the framework were immobilized ...
DOI:
10.1016/j.talanta.2020.121840

4. Quantification of CRP in human serum using a handheld fluorescence detection system for capillary-based ELISA

Author:
Morioka, Kazuhiro; Sato, Hina; Kuboyama, Minori; Yanagida, Akio; Shoji, Atsushi
Source:
Talanta 2021 v.224 pp. 121725
ISSN:
0039-9140
Subject:
enzyme-linked immunosorbent assay,   etc   ; C-reactive protein; blood serum; detection limit; fluorescence; humans; spectrometers;     Show all 7 Subjects
Abstract:
... We developed a handheld fluorescence detection system for capillary-based enzyme-linked immunosorbent assay (ELISA). The detection system implements both a long-pass filter and perpendicular optical arrangement, i.e., a power LED and a palm-sized spectrometer, to minimize background signals from the excitation light and optical scattering. The lower detection limit for resorufin was 0.13 μM. The d ...
DOI:
10.1016/j.talanta.2020.121725

5. FRET-based fluorometry assay for curcumin detecting using PVP-templated Cu NCs

Author:
Yao, Zhixia; Liu, Hanmeng; Liu, Yaosheng; Diao, Yongxing; Hu, Guangxing; Zhang, Qifang; Li, Zhuang
Source:
Talanta 2021 v.223 pp. 121741
ISSN:
0039-9140
Subject:
assays,   etc   ; curcumin; detection limit; energy transfer; exhibitions; fluorescence; fluorescent dyes; fluorometry;     Show all 8 Subjects
Abstract:
... A novel fluorometric strategy is proposed for detecting curcumin by polyvinyl pyrrolidone-templated Cu NCs (PVP–Cu NCs) as a fluorescent probe which exhibits excitation/emission peaks at 380/510 nm. The fluorescent excitation and emission spectra of PVP-Cu NCs have a striking overlap with the UV–vis spectrum of curcumin, and the fluorescence lifetime of PVP-Cu NCs decreases after the addition of c ...
DOI:
10.1016/j.talanta.2020.121741

6. Fighting and aggressive sound determines larger male to win male‐male competition in a bark beetle

Author:
Liu, Zhu‐Dong; Wickham, Jacob D.; Sun, Jiang‐Hua
Source:
Insect science 2021 v.28 no.1 pp. 203-214
ISSN:
1672-9609
Subject:
assays,   etc   ; Dendroctonus valens; animals; bark beetles; body size; females; males; monogamy; pressure; research; sexual selection; sounds;     Show all 12 Subjects
Abstract:
... Intrasexual selection occurs in male‐male competition over access to females and usually results in the larger male winning. While much research has documented that size matters, little is known about how the larger male wins. Dendroctonus valens is an aggregating monogamous bark beetle in which males have large variation in body size and display intense competition over females. Behavioral observ ...
DOI:
10.1111/1744-7917.12748

7. Four new cycloartane triterpenoids from the leaves of Dysoxylum binectariferum

Author:
Yan, Hui-jiao; Si, Hong-li; Zhao, Hong-wei; Chen, Long; Yu, Jin-qian; Zhao, Heng-qiang; Wang, Xiao
Source:
Phytochemistry letters 2021 v.41 pp. 101-105
ISSN:
1874-3900
Subject:
assays,   etc   ; Dysoxylum gotadhora; apoptosis; cells; cytotoxicity; leaves; plant biochemistry; spectral analysis; staining; triterpenoids;     Show all 10 Subjects
Abstract:
... Four new cycloartane triterpenoids (1-4) were isolated from the leaves of Dysoxylum binectariferum. Their structures were elucidated on the basis of HRMS and NMR spectroscopic data. Compounds 1-3 represent a class of rare natural 24,25,26,27-tetranor type cycloartane triterpenoids. Compound 3 displayed moderate cytotoxicity against HCT-116 and DLD-1 with IC₅₀ values of 9.8 ± 0.3 μM and 12.0 ± 0.4 ...
DOI:
10.1016/j.phytol.2020.11.013

8. Tirucallane triterpenoids from the mastic (Pistacia lentiscus) and their anti-inflammatory and cytotoxic activities

Author:
Liu, Wei; Gao, Jie; Li, Miaomiao; Aisa, Haji Akber; Yuan, Tao
Source:
Phytochemistry 2021 v.182 pp. 112596
ISSN:
0031-9422
Subject:
assays,   etc   ; Pistacia lentiscus; cytotoxicity; dexamethasone; doxorubicin; human cell lines; inhibitory concentration 50; phytochemicals; spectral analysis; triterpenoids;     Show all 10 Subjects
Abstract:
... Phytochemical investigation of mastic, a resin produced by Pistacia lentiscus, led to the isolation and identification of 16 tirucallane triterpenoids, 13 (1–13) of which are undescribed. Their structures and absolute configurations were elucidated based on the analysis of spectroscopic data and single-crystal X-ray diffraction. Compounds 12 and 13 are unusual 7(8 → 9)abeo-tirucallane triterpenoid ...
DOI:
10.1016/j.phytochem.2020.112596

9. Deep eutectic solvents-assisted synthesis of ZnCo2O4 nanosheets as peroxidase-like nanozyme and its application in colorimetric logic gate

Author:
Chen, Jia; Wei, Xin; Tang, Hao; Munyemana, Jean Claude; Guan, Ming; Zhang, Shusheng; Qiu, Hongdeng
Source:
Talanta 2021 v.222 pp. 121680
ISSN:
0039-9140
Subject:
assays,   etc   ; absorbance; catalytic activity; colorimetry; fields; food safety; measurement; nanosheets; oxidation; peroxidase; solvents; synthesis; therapeutics;     Show all 13 Subjects
Abstract:
... Pyrophosphate (PPi) and pyrophosphatase (PPase) play a significant role in the therapy of arthritic and clinical diagnosis. In this study, we quickly synthesized ZnCo₂O₄ nanosheets (NSs) with excellent peroxidase catalytic activity in basic deep eutectic solvent (DES). It was found that PPi could inhibit the catalytic capability of ZnCo₂O₄ NSs transforming colorless o-phenylenediamine (OPD) into y ...
DOI:
10.1016/j.talanta.2020.121680

10. In-vitro and in-vivo evaluation of the molecular mechanisms involved in the toxicity associated to CdSe/ZnS quantum dots exposure

Author:
Garcia-Calvo, E.; Cabezas-Sanchez, P.; Luque-Garcia, J.L.
Source:
Chemosphere 2021 v.263 pp. 128170
ISSN:
0045-6535
Subject:
assays,   etc   ; Danio rerio; apoptosis; cells; environment; hypoxia; industry; larvae; models; organisms; proteins; proteomics; quantum dots; toxicity;     Show all 14 Subjects
Abstract:
... The use of different types of quantum dots is growing in recent times in both the technology and biomedical industries. Such is the extension of the use of these quantum dots that they have become potential emerging contaminants, which makes it necessary to evaluate their potential toxicity and the impact they may have on both health and the environment. Although studies already exist in this rega ...
DOI:
10.1016/j.chemosphere.2020.128170

11. Potent inhibitory activity of taniborbactam towards NDM-1 and NDM-1Q119X mutants, and in vitro activity of cefepime/taniborbactam against MBLs producing Enterobacterales

Author:
Piccirilli, Alessandra; Segatore, Bernardetta; Brisdelli, Fabrizia; Amicosante, Gianfranco; Perilli, Mariagrazia
Source:
International journal of antimicrobial agents 2021 v.57 no.1 pp. 106228
ISSN:
0924-8579
Subject:
assays,   etc   ; Escherichia coli; anti-infective agents; antibacterial properties; enzymes; laboratories; minimum inhibitory concentration; mutants; phenotype; strains;     Show all 10 Subjects
Abstract:
... Objective: This study aimed to investigate the in vitro activity of taniborbactam (VNRX-5133), a novel broad-spectrum bicyclic boronate, against NDM-1 and Q119E, Q119K, Q119C, Q119F, Q119V, and Q119Y NDM-1 variants, which showed an increased activity towards some β-lactams, including cefepime.Methods: Inhibition kinetic assays were spectrophotometrically performed using cefepime (50 μM) as the rep ...
DOI:
10.1016/j.ijantimicag.2020.106228

12. Microdevice immunoassay with conjugated magnetic nanoparticles for rapid anti-cyclic citrullinated peptide (anti-CCP) detection

Author:
Ramos, Kenia Chávez; Macías, María del Pilar Cañizares
Source:
Talanta 2021 v.224 pp. 121801
ISSN:
0039-9140
Subject:
enzyme-linked immunosorbent assay,   etc   ; autoantibodies; biomarkers; design; detection limit; flow; immune response; magnetism; nanoparticles; optical fibers; peptides; rheumatoid arthritis; sampling;     Show all 13 Subjects
Abstract:
... Anti-cyclic citrullinated peptide IgG antibodies (anti-CCP) are produced as an immune response in the presence of post-translational modified peptides known as cyclic citrullinated peptides (CCP). Anti-CCP have been considered as specific biomarkers for the diagnosis of rheumatoid arthritis (RA), and due to their high specificity, it is possible to make a differential diagnosis of other rheumatic ...
DOI:
10.1016/j.talanta.2020.121801

13. Association between positivity of serum autoantibodies and liver disease severity in patients with biopsy-proven NAFLD

Author:
Zhou, Yu-Jie; Zheng, Kenneth I.; Ma, Hong-Lei; Li, Gang; Pan, Xiao-Yan; Zhu, Pei-Wu; Targher, Giovanni; Byrne, Christopher D.; Wang, Xiao-Dong; Chen, Yong-Ping; Li, Xiao-Bo; Zheng, Ming-Hua
Source:
Nutrition, metabolism, and cardiovascular diseases 2021 v.31 no.2 pp. 552-560
ISSN:
0939-4753
Subject:
assays,   etc   ; autoantibodies; blood serum; cardiovascular diseases; disease severity; fatty liver; fibrosis; genotype; immunoblotting; liver cirrhosis; metabolism; nutrition; patients; regression analysis;     Show all 14 Subjects
Abstract:
... Some previous studies reported serum autoantibody positivity in patients with nonalcoholic fatty liver disease (NAFLD). The clinical significance of these findings remains uncertain. We aimed to investigate the association between the presence of serum autoantibodies and liver disease severity in NAFLD.A total of 388 consecutive patients with biopsy-proven NAFLD were included in the study. Various ...
DOI:
10.1016/j.numecd.2020.10.004

14. UHPLC-MS/MS analysis of cannabidiol metabolites in serum and urine samples. Application to an individual treated with medical cannabis

Author:
Pichini, Simona; Malaca, Sara; Gottardi, Massimo; Pérez-Acevedo, Ana Pilar; Papaseit, Esther; Perez-Maña, Clara; Farré, Magí; Pacifici, Roberta; Tagliabracci, Adriano; Mannocchi, Giulio; Busardò, Francesco Paolo
Source:
Talanta 2021 v.223 pp. 121772
ISSN:
0039-9140
Subject:
assays,   etc   ; Cannabis; blood serum; cannabidiol; drugs; ionization; metabolites; pharmacodynamics; pharmacokinetics; sampling; tandem mass spectrometry; ultra-performance liquid chromatography; urine;     Show all 13 Subjects
Abstract:
... No analytical assay is currently available for the simultaneous determination of CBD major metabolites in serum or urine samples of individuals treated with medical cannabis or CBD-based pharmaceuticals. We developed and validated a method using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC–MS/MS) for quantifying cannabidiol (CBD) and its metabolites, ca ...
DOI:
10.1016/j.talanta.2020.121772

15. Chemical constituents from the leaves of Styrax argentifolius H.L. Li and their biological activities

Author:
Tra, Nguyen Thanh; Van Tuyen, Nguyen; Van Cuong, Pham; Ha, Nguyen Thi Thu; Anh, Le Thi Tu; Son, Ninh The
Source:
Phytochemistry letters 2021 v.41 pp. 70-73
ISSN:
1874-3900
Subject:
assays,   etc   ; Styrax; acarbose; benzofurans; carbon; cell lines; chemical composition; cytotoxicity; leaves; lignans; neoplasm cells; plant biochemistry; sterols; triterpenoids;     Show all 14 Subjects
Abstract:
... Searching for bioactive agents from Vietnamese plants, nine compounds, including five nor-lignan-type benzofurans 1–5, two lignans 6 and 7, one sterol 8 and one triterpenoid 9 were separated from Styrax argentifolius for the first time. 5-((E)-2-Carboxyvinyl)-7-methoxy-2-(3′,4′-methylenedioxyphenyl)benzofuran (1) and 5-carboxy-7-methoxy-2-(3′,4′-methylenedioxyphenyl)benzofuran (2) were new benzofu ...
DOI:
10.1016/j.phytol.2020.11.003

16. Design and evaluation of an HACCP gluten-free protocol in a children's hospital

Author:
Vukman, Diana; Viličnik, Petra; Vahčić, Nada; Lasić, Dario; Niseteo, Tena; Panjkota Krbavčić, Ines; Marković, Ksenija; Bituh, Martina
Source:
Food control 2021 v.120 pp. 107527
ISSN:
0956-7135
Subject:
enzyme-linked immunosorbent assay,   etc   ; HACCP; celiac disease; children; design; gluten; gluten-free diet; gluten-free foods; hospitals; patients; protocols;     Show all 11 Subjects
Abstract:
... For patients with gluten intolerance, the most common cause of treatment failure is an inadequate gluten-free diet (GFD), such as because of gluten contamination. Here we describe the design, implementation and validation of an HACCP protocol for preparing gluten-free meals in a children's hospital. Gluten content in prepared meals was analyzed using R5 sandwich ELISA, which indicated that gluten ...
DOI:
10.1016/j.foodcont.2020.107527

17. Evaluation of a real-time PCR assay for the differentiation of Bacillus cereus group species

Author:
Frentzel, Hendrik; Kelner-Burgos, Ylanna; Deneke, Carlus
Source:
Food control 2021 v.120 pp. 107530
ISSN:
0956-7135
Subject:
assays,   etc   ; Bacillus cereus; Bacillus weihenstephanensis; detection; food safety; multilocus sequence typing; nucleotide sequences; quantitative polymerase chain reaction; temperature;     Show all 9 Subjects
Abstract:
... For the differentiation of the species Bacillus weihenstephanensis and B. pseudomycoides from other members of the B. cereus group a real-time PCR was suggested in 2011, which applies the primer-probe-combinations BCFomp2/BCRomp2 with MotB-FAM-2 and BpmF/BpmR2 with Bpm-FAM-1, respectively. However, a subsequent study revealed contradictions between taxonomic relationships based on real-time PCR re ...
DOI:
10.1016/j.foodcont.2020.107530

18. Optimization and evaluation of the ARCHITECT Chagas assay and in-house ELISA for Chagas disease in clinical settings in Japan

Author:
Imai, Kazuo; Murakami, Takashi; Misawa, Kazuhisa; Fujikura, Yuji; Kawana, Akihiko; Tarumoto, Norihito; Maesaki, Shigefumi; Maeda, Takuya
Source:
Parasitology international 2021 v.80 pp. 102221
ISSN:
1383-5769
Subject:
assays,   etc   ; Chagas disease; Japan; Trypanosoma cruzi; blood serum; diagnostic techniques; false positive results; immigration; parasitology; recombinant proteins; sampling; Latin America;     Show all 12 Subjects
Abstract:
... Approximately 250,000 immigrants from Latin America live in Japan and it is estimated that 1500–3000 of them are potentially infected with Trypanosoma cruzi, the cause of Chagas disease. Therefore, the establishment of a standardized diagnostic method for Chagas disease in Japan is urgently needed. In this study, we optimized and evaluated the ARCHITECT Chagas assay and in-house ELISA for Chagas d ...
DOI:
10.1016/j.parint.2020.102221

19. Vinapraesorediosic acids D and E from the lichen Parmotrema praesorediosum (Nyl.) Hale

Author:
Huynh, Bui-Linh-Chi; Pham, Nguyen Kim Tuyen; Nguyen, Tan Phat
Source:
Phytochemistry letters 2021 v.41 pp. 61-64
ISSN:
1874-3900
Subject:
assays,   etc   ; Parmotrema; benzoic acid; cell lines; chemical structure; cytotoxicity; humans; lichens; neoplasm cells; plant biochemistry; spectral analysis;     Show all 11 Subjects
Abstract:
... One new α,β-unsaturated-γ-lactone acid, named Vinapraesorediosic acid D (1), and one new benzoic acid, Vinapraesorediosic acid E (2), were isolated from the lichen Parmotrema praesorediosum. Their chemical structures were elucidated by extensive IR, CD, HR-ESI-MS, and NMR spectroscopic analysis, and comparison with previously published data. Compound 1 was evaluated for the cytotoxicity against fo ...
DOI:
10.1016/j.phytol.2020.11.001

20. Detection of membrane receptors on per tumor cell by nonimmobilized cell capillary electrophoresis and a mathematic model

Author:
Wu, Ruijun; Liang, Chunsu; Zou, Juncheng; Li, Cong; Yan, Youqi; Hu, Xin; Ling, Xiaomei
Source:
Talanta 2021 v.222 pp. 121425
ISSN:
0039-9140
Subject:
enzyme-linked immunosorbent assay,   etc   ; capillary electrophoresis; cell membranes; folate receptors; mathematical models; neoplasm cells; neoplasms; receptors; therapeutics;     Show all 9 Subjects
Abstract:
... Folate receptors (FRs) are a class of valuable therapeutic target which is highly expressed on a variety of cancers. The accurate detection of the expression of FRs in different cells is conducive to improve the accuracy of FR targeted tumor therapy. Herein, a method based on nonimmobilized cell capillary electrophoresis (NICCE) combined with a mathematic model to quantify FRs on each single tumor ...
DOI:
10.1016/j.talanta.2020.121425