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1. Effects of molecular crowding on a bimolecular group I ribozyme and its derivative that self-assembles to form ribozyme oligomers

Author:
Rahman, Md Motiar; Matsumura, Shigeyoshi; Ikawa, Yoshiya
Source:
Biochemical and biophysical research communications 2018 v.507 no.1-4 pp. 136-141
ISSN:
0006-291X
Subject:
assays,   etc   ; mutation; ribozymes;     Show all 3 Subjects
Abstract:
... In the RNA world, enrichment of self-replicating RNAs would have been beneficial to their survival, amplification, and evolution. Self-assembly of RNAs may be a strategy by which they enrich themselves. We examined the effects of molecular crowding on the activity of a bimolecular group I ribozyme and its derivative that self-assembles to form ribozyme oligomers. In a comparative activity assay us ...
DOI:
10.1016/j.bbrc.2018.10.188

2. Correction to: Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies

Author:
Nurtop, Elif; Villarroel, Paola Mariela Saba; Pastorino, Boris; Ninove, Laetitia; Drexler, Jan Felix; Roca, Yelin; Gake, Bouba; Dubot-Peres, Audrey; Grard, Gilda; Peyrefitte, Christophe; Priet, Stéphane; de Lamballerie, Xavier; Gallian, Pierre
Source:
Virology journal 2019 v.16 no.1 pp. 12
ISSN:
1743-422X
Subject:
enzyme-linked immunosorbent assay,   etc   ; Zika virus; screening; seroprevalence;     Show all 4 Subjects
Abstract:
... In the original publication of this article [1], Table 1 has some errors. ...
DOI:
10.1186/s12985-019-1118-8

3. Development of the Com1 synthetic peptide-based Latex Agglutination Test (LAT) and its comparative evaluation with commercial indirect-ELISA for sero-screening of coxiellosis in cattle

Author:
Kumar, Manesh; Malik, Satyaveer Singh; Vergis, Jess; Ramanjeneya, Sunitha; Sahu, Radhakrishna; Pathak, Richa; Yadav, Jay Prakash; Dhaka, Pankaj; Barbuddhe, Sukhadeo B.; Rawool, Deepak B.
Source:
Journal of microbiological methods 2019 v.162 pp. 83-85
ISSN:
0167-7012
Subject:
enzyme-linked immunosorbent assay,   etc   ; Q fever; cattle; latex agglutination test;     Show all 4 Subjects
Abstract:
... A novel Com1 synthetic peptide-based latex agglutination test (LAT) was developed and evaluated against commercial ELISA kit for sero-screening of coxiellosis in cattle. The developed test is economical, has field applicability and can serve as an important rapid tool for sero-screening of coxiellosis in cattle. ...
DOI:
10.1016/j.mimet.2019.05.012

4. Soil algae pipe assay: ex situ method for the evaluation of soil quality based on soil algae and its application to the pot test

Author:
Kwak, Jin Il; Nam, Sun-Hwa; An, Youn-Joo
Source:
Chemosphere 2019 v.224 pp. 634-636
ISSN:
0045-6535
Subject:
assays,   etc   ; algae; flow cytometry; greenhouses; soil; soil quality; toxicity testing;     Show all 7 Subjects
Abstract:
... We aimed to develop an ex situ method for evaluating soil quality using soil algae. The soil algae pipe assay involves application and sampling of algae and flow cytometry. As it can be performed in a greenhouse, the assay was effective for pot tests and conducive for soil quality assessment. This novel assay will enable broader toxicity testing. ...
DOI:
10.1016/j.chemosphere.2019.02.198

5. Current trends in rapid tests for mycotoxins

Author:
Nolan, Philana; Auer, Sanna; Spehar, Anna; Elliott, Christopher T.; Campbell, Katrina
Source:
Food additives & contaminants 2019 v.36 no.5 pp. 800-814
ISSN:
1944-0057
Subject:
enzyme-linked immunosorbent assay,   etc   ; biosensors; fluorescence; fluorometry; markets; mycotoxins;     Show all 6 Subjects
Abstract:
... There are an ample number of commercial testing kits available for mycotoxin analysis on the market today, including enzyme-linked immunosorbent assays, membrane-based immunoassays, fluorescence polarisation immunoassays and fluorometric assays. It can be observed from the literature that not only are developments and improvements ongoing for these assays but there are also novel assays being deve ...
DOI:
10.1080/19440049.2019.1595171

6. Doramectin induced cytotoxic and genotoxic effects on bovine peripheral lymphocytes and cumulus cells in vitro

Author:
Anchordoquy, Juan Mateo; Anchordoquy, Juan Patricio; Nikoloff, Noelia; Gambaro, Rocío; Padula, Gisel; Seoane, Analía; Furnus, Cecilia
Source:
Journal of environmental science and health 2019 v.54 no.2 pp. 147-154
ISSN:
1532-4109
Subject:
comet assay,   etc   ; DNA damage; cattle; cytotoxicity; doramectin; genotoxicity; lymphocytes; mutagens;     Show all 8 Subjects
Abstract:
... The effect of doramectin (DOR) was tested on two experimental somatic bovine cells in vitro: peripheral lymphocytes (PL) and cumulus cells (CC). The cytotoxicity and genotoxicity of DOR were assessed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, single cell gel electrophoresis assay (SCGE) and cytokinesis-block micronucleus cytome (CBMN Cyt) assay. Both cells ...
DOI:
10.1080/03601234.2018.1559569

7. Antioxidant activity of proteins extracted from red alga dulse harvested in Japan

Author:
Sato, Naoto; Furuta, Tomoe; Takeda, Tomoyuki; Miyabe, Yoshikatsu; Ura, Kazuhiro; Takagi, Yasuaki; Yasui, Hajime; Kumagai, Yuya; Kishimura, Hideki
Source:
Journal of food biochemistry 2019 v.43 no.2 pp. e12709
ISSN:
0145-8884
Subject:
viability assays,   etc   ; Palmaria; antioxidant activity; apoproteins; cell viability; phycoerythrin; Japan;     Show all 7 Subjects
Abstract:
... In this study, we investigated antioxidant activity of proteins from the red alga dulse (Palmaria sp.) harvested in Hokkaido, Japan. The dulse proteins that contain phycoerythrin (PE) as the main component showed a high radical scavenging activity. To clarify the key constituent of antioxidant activity in dulse proteins, we prepared recombinant dulse PE β‐subunit (rPEβ) (apoprotein) and chromophor ...
DOI:
10.1111/jfbc.12709

8. Multiplex detection of microRNAs by combining molecular beacon probes with T7 exonuclease-assisted cyclic amplification reaction

Author:
Liu, Yacui; Zhang, Jiangyan; Tian, Jingxiao; Fan, Xiaofei; Geng, Hao; Cheng, Yongqiang
Source:
Analytical and bioanalytical chemistry 2017 v.409 no.1 pp. 107-114
ISSN:
1618-2642
Subject:
assays,   etc   ; detection; fluorescence; microRNA;     Show all 4 Subjects
Abstract:
... A simple, highly sensitive, and specific assay was developed for the homogeneous and multiplex detection of microRNAs (miRNAs) by combining molecular beacon (MB) probes and T7 exonuclease-assisted cyclic amplification. An MB probe with five base pairs in the stem region without special modification can effectively prevent the digestion by T7 exonuclease. Only in the presence of target miRNA is the ...
DOI:
10.1007/s00216-016-0027-6

9. Streamlining standard bacteriophage methods for higher throughput

Author:
Kauffman, Kathryn M.; Polz, Martin F.
Source:
MethodsX 2018 v.5 pp. 159-172
ISSN:
2215-0161
Subject:
assays,   etc   ; agar; bacteriophages; purification methods; rapid methods;     Show all 5 Subjects
Abstract:
... A universal tool in the culture-based study of bacterial viruses (bacteriophages, or phages) is the agar overlay, which is used in the isolation of new viruses, and in their quantification and purification. Here, simple optimizations that increase efficiency and throughput in agar overlay based isolation and cultivation of virus-host systems are presented. The agar overlay is streamlined to minimi ...
DOI:
10.1016/j.mex.2018.01.007

10. Investigation of the anti-apoptotic activity of ozone therapy in rainbow trout macrophages infected with Yersinia ruckeri

Author:
Küçükgül, Azime; Küçükgül, Altuğ; Gönenci, Ramazan; Özsoy, Şule Yurdagül; Kutlu, Banu; İşgör, Mehmet Mustafa
Source:
Aquaculture international 2019 v.27 no.3 pp. 771-783
ISSN:
0967-6120
Subject:
enzyme-linked immunosorbent assay; viability assays,   etc   ; Oncorhynchus mykiss; Yersinia ruckeri; acridine orange; apoptosis; bacteria; caspases; ethidium; macrophages; ozone; protein content; quantitative polymerase chain reaction; staining; therapeutics; trout; yersiniosis;     Show all 17 Subjects
Abstract:
... Yersinia ruckeri is the causal agent of enteric redmouth (ERM) in trout. This study aimed to investigate the effectiveness of nontoxic ozone on Y. ruckeri–infected trout cells in in vitro conditions. Rainbow trout macrophages (RTS-11) were exposed to Y. ruckeri for 24 h after being incubated with the ozonized medium at nontoxic concentration. Effective concentrations of nontoxic ozone (3 gamma) an ...
DOI:
10.1007/s10499-019-00364-8

11. Repeated immunosensing by a dithiobis(succinimidyl propionate)-modified SAW device

Author:
Toma, Koji; Oishi, Koki; Yoshimura, Naoyuki; Arakawa, Takahiro; Yatsuda, Hiromi; Mitsubayashi, Kohji
Source:
Talanta 2019 v.203 pp. 274-279
ISSN:
0039-9140
Subject:
enzyme-linked immunosorbent assay,   etc   ; adsorption; antibodies; antigens; gold; immunosensors; mice; models; propionic acid; quartz;     Show all 10 Subjects
Abstract:
... The possibility of dithiobis(succinimidyl propionate) (DSP) for repeated immunoassay with a surface acoustic wave (SAW) immunosensor was explored. In the sensor, DSP was used to modify a gold-coated quartz sensing area of a SAW device by forming a self-assembled monolayer on the gold surface. In a model sandwich assay using mouse (mIgG) and anti-mouse (a-mIgG) antibodies, the primary antibody, mIg ...
DOI:
10.1016/j.talanta.2019.05.080

12. Comparison of recovery and immunochemical detection of peanut proteins from differentially roasted peanut flour using ELISA

Author:
Jayasena, Shyamali; Koppelman, Stef J.; Nayak, Balunkeswar; Taylor, Steve L.; Baumert, Joseph L.
Source:
Food chemistry 2019 v.292 pp. 32-38
ISSN:
0308-8146
Subject:
enzyme-linked immunosorbent assay,   etc   ; antibodies; heat; immunochemistry; peanut flour; peanut protein; peanuts; protein solubility; roasting;     Show all 9 Subjects
Abstract:
... The effect of heat on extractability and immunoreactivity of proteins from roasted peanut flours and whole peanuts was evaluated using two general protein assays and six commercial peanut ELISA kits, respectively. The highest amount of protein was recovered from roasted peanuts with all ELISAs, while recovery showed a decrease with increasing levels of roasting of the peanut flours. Only the Morin ...
DOI:
10.1016/j.foodchem.2019.04.026

13. Knockdown of lncRNA MALAT1 alleviates bupivacaine-induced neurotoxicity via the miR-101-3p/PDCD4 axis

Author:
Zhao, Yanling; Ai, Yanqiu
Source:
Life sciences 2019 v.232 pp. 116606
ISSN:
0024-3205
Subject:
bioluminescence assay,   etc   ; Western blotting; apoptosis; cell viability; local anesthetics; mice; models; neurons; neurotoxicity; survival rate;     Show all 10 Subjects
Abstract:
... Bupivacaine, a common local anesthetic, can cause neurotoxicity and abnormal neuro-disorders. However, the precise underlying mechanisms have not been fully elucidated. In this study, we investigated the function of lncRNA MALAT1 in the bupivacaine-induced neurotoxicity process.SH-SY5Y cells and neonatal mouse DRG neurons were cultured in vitro and treated with bupivacaine to establish a neurotoxi ...
DOI:
10.1016/j.lfs.2019.116606

14. Development of sandwich and competitive ELISA formats to determine β-conglycinin: Evaluation of their performance to detect soy in processed food

Author:
Segura-Gil, Isabel; Blázquez-Soro, Arturo; Galán-Malo, Patricia; Mata, Luis; Tobajas, Ana P.; Sánchez, Lourdes; Pérez, María D.
Source:
Food control 2019 v.103 pp. 78-85
ISSN:
0956-7135
Subject:
enzyme-linked immunosorbent assay,   etc   ; allergens; beta-conglycinin; ingredients; models; pasteurization; processed foods; prototypes; sausages; soy protein;     Show all 10 Subjects
Abstract:
... Two ELISA formats (sandwich and indirect competitive) were developed to quantify β-conglycinin, a major soy allergen. Their performance was evaluated using three model foods incurred with soy proteins. The sandwich format detects the addition of 0.005% and 0.05% soy proteins in pasteurized sausages and baked bread. However, the competitive format detects only 0.1 and 0.5%, respectively. β-conglyci ...
DOI:
10.1016/j.foodcont.2019.03.035

15. Diagnostic and follow-up performance of serological tests for different forms/courses of alveolar echinococcosis

Author:
Gottstein, Bruno; Lachenmayer, Anja; Beldi, Guido; Wang, Junhua; Merkle, Bernadette; Vu, Xuan Lan; Kurath, Ursula; Müller, Norbert
Source:
Food and Waterborne Parasitology 2019 v.16 pp. e00055
ISSN:
2405-6766
Subject:
enzyme-linked immunosorbent assay,   etc   ; benzimidazole; diagnostic sensitivity; drug therapy; echinococcosis; image analysis; monitoring; patients; surgery;     Show all 9 Subjects
Abstract:
... Diagnosis of alveolar echinococcosis (AE) is predominantly based on imaging procedures combined with immunodiagnostic testing. In the present study, we retrospectively analyzed the performance of four serological tests (EgHF-ELISA, Em2-ELISA, recEm18-ELISA and Em-Immunoblotting) for initial diagnosis and subsequent monitoring of AE patients. Overall, 101 AE patients were included, grouped accordin ...
DOI:
10.1016/j.fawpar.2019.e00055

16. Parietaria major allergens vs pollen in the air we breathe

Author:
De Linares, Concepción; Alcázar, Purificación; Valle, Ana M.; Díaz de la Guardia, Consuelo; Galán, Carmen
Source:
Environmental research 2019 v.176 pp. 108514
ISSN:
0013-9351
Subject:
enzyme-linked immunosorbent assay,   etc   ; Parietaria; Urtica; aerodynamics; air; allergens; cities; hay fever; particulates; pollen; pollination; Spain;     Show all 12 Subjects
Abstract:
... Parietaria and Urtica are the genera from the Urticaceae family more frequent in Mediterranean and Atlantic areas. Moreover, both genera share pollination periods, and their pollen (of the main species) is so similar that there is no aerobiological evidence of the proportion of each of them in the airborne pollen identification, except in the case of U. membranacea. However, Parietaria is one of t ...
DOI:
10.1016/j.envres.2019.05.045

17. Performance of the commercially available SERION ELISA classic Echinococcus IgG test for the detection of cystic echinococcosis in clinical practice

Author:
Sarink, M.J.; Koelewijn, R.; Slingerland, B.C.G.C.; Tielens, A.G.M.; van Genderen, P.J.J.; van Hellemond, J.J.
Source:
Journal of helminthology 2019 v.93 no.5 pp. 636-639
ISSN:
1475-2697
Subject:
enzyme-linked immunosorbent assay,   etc   ; Echinococcus; blood serum; echinococcosis; image analysis; immunoglobulin G; infectious diseases; patients; serology;     Show all 9 Subjects
Abstract:
... Diagnosis of cystic echinococcosis (CE) is at present mainly based on imaging techniques. Serology has a complementary role, partly due to the small number of standardized and commercially available assays. Therefore we examined the clinical performance of the SERION ELISA classic Echinococcus IgG test. Using 10 U/ml as a cut-off point, and serum samples from 50 CE patients and 105 healthy control ...
DOI:
10.1017/S0022149X18000536
PubMed:
29950187

18. Long-term immunogenicity of measles, mumps and rubella-containing vaccines in healthy young children: A 10-year follow-up

Author:
Carryn, Stephane; Feyssaguet, Muriel; Povey, Michael; Di Paolo, Emmanuel
Source:
Vaccine 2019 v.37 no.36 pp. 5323-5331
ISSN:
0264-410X
Subject:
enzyme-linked immunosorbent assay,   etc   ; antibodies; antibody formation; children; immunogenicity; measles; seroprevalence; vaccination; vaccines; viruses;     Show all 10 Subjects
Abstract:
... Measles and mumps outbreaks still occur in countries that have successfully implemented universal routine immunization programs. Measles outbreaks are mostly associated to absent or incomplete vaccination, whereas for mumps outbreaks the combined effects of waning of immunity and circulating new strains are incriminated. It is therefore increasingly useful to characterize the long-lasting immunity ...
DOI:
10.1016/j.vaccine.2019.07.049

19. Cloning and identification of Bartonella α-enolase as a plasminogen-binding protein

Author:
Deng, Hongkuan; Wu, Suge; Song, Qian; Zhang, Junshi; Sang, Feng; Sun, Xiaoyue; Xu, Tong; Gao, Yan; Zhao, Bosheng
Source:
Microbial pathogenesis 2019
ISSN:
0882-4010
Subject:
enzyme-linked immunosorbent assay,   etc   ; Bartonella henselae; bacteriophages; computer simulation; humans; phosphopyruvate hydratase; plasminogen; public health;     Show all 8 Subjects
Abstract:
... Bartonella infection is distributed worldwide with animal and public health. Recent studies have shown that host cells infection by Bartonella has a series of different infection stages, beginning with encounter and adherence to the cells. In this study, we expressed and purified recombinant Bartonella henselae (B. henselae) α-enolase. And we found that B. henselae α-enolase is highly conserved in ...
DOI:
10.1016/j.micpath.2019.103651

20. Determination of Fucose Concentration in a Lectin-Based Displacement Microfluidic Assay

Author:
Erlandsson, Per G.; Åström, Eva; Påhlsson, Peter; Robinson, Nathaniel D.
Source:
Applied biochemistry and biotechnology 2019 v.188 no.3 pp. 868-877
ISSN:
0273-2289
Subject:
enzyme-linked immunosorbent assay,   etc   ; Aleuria; binding sites; fluorescence; fucose; glycoproteins; lactoferrin; lectins; polypeptides;     Show all 9 Subjects
Abstract:
... We compare three different methods to quantify the monosaccharide fucose in solutions using the displacement of a large glycoprotein, lactoferrin. Two microfluidic analysis methods, namely fluorescence detection of (labeled) lactoferrin as it is displaced by unlabeled fucose and the displacement of (unlabeled) lactoferrin in SPR, provide fast responses and continuous data during the experiment, th ...
DOI:
10.1007/s12010-018-02944-5