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Wanding Zhou; Toshinori Hinoue; Bret Barnes; Owen Mitchell; Waleed Iqbal; Sol Moe Lee; Kelly K. Foy; Kwang-Ho Lee; Ethan J. Moyer; Alexandra VanderArk; Julie M. Koeman; Wubin Ding; Manpreet Kalkat; Nathan J. Spix; Bryn Eagleson; John Andrew Pospisilik; Piroska E. Szabó; Marisa S. Bartolomei; Nicole A. Vander Schaaf; Liang Kang; Ashley K. Wiseman; Peter A. Jones; Connie M. Krawczyk; Marie Adams; Rishi Porecha; Brian H. Chen; Hui Shen; Peter W. Laird
backcrossing, etc ; DNA; DNA methylation; carcinogenesis; genomics; intestines; mice; xenotransplantation; Show all 8 Subjects
Abstract:
... We have developed a mouse DNA methylation array that contains 296,070 probes representing the diversity of mouse DNA methylation biology. We present a mouse methylation atlas as a rich reference resource of 1,239 DNA samples encompassing distinct tissues, strains, ages, sexes, and pathologies. We describe applications for comparative epigenomics, genomic imprinting, epigenetic inhibitors, patient- ...
Tej Pratap Jitendra Kumar; Anjali Rai; Sumit K. Singh; Rajeev Ranjan Kumar; Arvind K. Ahlawat; Savita Saini; R. B. Shukla; Namita Bedi; Anju Mahendru-Singh
backcrossing, etc ; biotechnology; grain yield; hardness; heterozygosity; plant biochemistry; wheat; Show all 7 Subjects
Abstract:
... The genes for grain softness and rust resistance were transferred from a soft grained Australian wheat variety (Barham) to a hard grained Indian wheat variety (DBW14) through marker assisted back cross breeding. Backcrosses were made with recurrent parent in the BC₁F₁ plants that were found positive for markers of Pina-D1a, Pina-D1b and Lr37b genes. The BC₂F₁ plants were further screened and those ...
backcrossing, etc ; DNA; Ipomoea; diploidy; evolution; genotyping; quantitative traits; sweet potatoes; Show all 8 Subjects
Abstract:
... Ipomoea trifida, the closest relative of sweetpotato, does not generally form storage roots, but some lines develop thick roots similar to the storage roots of the sweetpotato. Revealing the molecular mechanism of thick root development in I. trifida would help us understand the evolution of storage roots in the sweetpotato. In this study, we conducted a mapping method for quantitative trait loci ...
backcrossing, etc ; corn; genetic distance; genetic similarity; genetically modified organisms; genome; hybrids; Show all 7 Subjects
Abstract:
... The marker assisted backcross (MABC) method is the most used to obtain transgenic hybrid and transgenic inbred lines in maize with few backcrosses (BC) generations. It is possible that the use of donor parents with greater genetic similarity with recurrent parents can further reduce the number of BC generation to recover recurrent parent genome. The objective of this study was to verify the influe ...
backcrossing, etc ; animals; biogenesis; corn; epigenetics; genome; genomics; hybridization; hybrids; methylation; selfing; Show all 11 Subjects
Abstract:
... BACKGROUND: Hybridization and backcrossing are commonly used in animal and plant breeding to induce heritable variation including epigenetic changes such as paramutation. However, the molecular basis for hybrid-induced epigenetic memory remains elusive. RESULTS: Here, we report that hybridization between the inbred parents B73 and Mo17 induces trans-acting hypermethylation and hypomethylation at t ...
backcrossing, etc ; Cucumis sativus; biotechnology; cucumbers; cultivars; early development; environment; horticulture; loci; monoecy; Show all 10 Subjects
Abstract:
... This study was conducted to develop inbred backcross lines (IBLs) in Indian slicing cucumber (Cucumis sativus L.) with the gynoecious trait. The F₂ mapping population was developed using the Indian monoecious cultivar of light-green-fruited Pusa Uday as the recurrent parent and the American gynoecious pickling line G421 as a donor. Two markers, SSR13251 and SSR15516, were found to be closely linke ...
backcrossing, etc ; Oryza sativa; genetic improvement; genetic variation; heritability; plant height; rice; seeds; Show all 8 Subjects
Abstract:
... Two BC₁F₂ populations derived from the cross CO 52 x 3-11-11-2 were evaluated in the present study for fifteen yields, yield contributing characters and quality characters to assess genetic variability, correlation coefficient and path coefficient analyses. Single plant yield, number of productive tillers per plant and alkali spreading value all had higher estimates of PCV and GCV. The high herita ...
backcrossing, etc ; epistasis; genome; genomics; heterosis; hybrids; inbred lines; introgression; phenotype; rice; China; Show all 11 Subjects
Abstract:
... BACKGROUND: Since its development and wide adoption in China, hybrid rice has reached the yield plateau for more than three decades. To understand the genetic basis of heterosis in rice and accelerate hybrid rice breeding, the yield performances of the elite rice hybrid, Quan-you-si-miao (QYSM) were genetically dissected by whole-genome sequencing, large-scale phenotyping of 1061 recombined inbred ...
backcrossing, etc ; DNA; chromosome mapping; color; cultivars; genomics; introgression; leaves; peaches; phenotype; progeny; Show all 11 Subjects
Abstract:
... Based on detailed maps, DNA sequences and phenotypic data, there is a great deal of information on the genetics and genomics of ‘Earlygold’, a historical peach cultivar from the US. The F₂ between ‘Texas’ almond and ‘Earlygold’ peach (T × E) was used to construct the first saturated peach linkage map that later became the reference map for the Prunus genus. This population and the first backcross ...
backcrossing, etc ; ancestry; dogs; genetic rescue; genetic variation; heterozygosity; homozygosity; outcrossing; single nucleotide polymorphism; Show all 9 Subjects
Abstract:
... Augmenting the genetic diversity of small, inbred populations by the introduction of new individuals is often termed “genetic rescue”. An example is the Norwegian Lundehund, a small spitz dog with inbreeding-related health problems that is being crossed with three Nordic breeds, including the Norwegian Buhund. Conservation breeding decisions for the (typically) small number of outcrossed individua ...
backcrossing, etc ; alleles; cultivars; epistasis; grain quality; grain yield; heterosis; introgression; overdominance; phenotype; rice; Show all 11 Subjects
Abstract:
... Development of hybrid rice with high yield and grain quality is a goal of rice breeding. To investigate the genetic mechanism of heterosis for rice milling and appearance quality in indica/xian rice, QTL mapping was conducted using 1061 recombinant inbred lines (RILs) derived from a cross of the xian rice cultivars Quan9311B (Q9311B) and Wu-shan-si-miao (WSSM), and a backcross F₁ (BC₁F₁) populatio ...
backcrossing, etc ; Apis mellifera carnica; ecology; hybridization; insemination; morphometry; queen honey bees; spermatheca; wings; Show all 9 Subjects
Abstract:
... Apis mellifera, the Western honeybee, is a widespread species that has differentiated into numerous geographical strains or subspecies. These subspecies differ in various characteristics, such as morphology, behavior, and ecology. In the present investigation, different morphometric characteristics of the Carniolan (Apis mellifera carnica) and Egyptian (Apis mellifera lamarckii) subspecies were co ...
backcrossing, etc ; Ambrosia artemisiifolia; biotypes; cross resistance; herbicide resistance; horticulture; linuron; metribuzin; progeny; weeds; Show all 10 Subjects
Abstract:
... The full spectrum of herbicide resistance in a weed can vary according to the mechanistic basis and cannot be implied from the selective pressure. Common ragweed (Ambrosia artemisiifolia L.) is an important weed species of horticultural crops that has developed resistance to linuron based on either target site– or non–target site resistance mechanisms. The objective of the study is to characterize ...
backcrossing, etc ; Oryza sativa; cultivars; genes; haplotypes; loci; promoter regions; rice; selfing; sequence alignment; Show all 10 Subjects
Abstract:
... Grain size influences the yield and quality of rice (Oryza sativa L.), and grain length is one of the component traits of grain size. In this study, a near-isogenic line LB3 with long grain size was constructed using japonica rice cultivar 02428, with short grain size, as the recipient parent and indica rice cultivar ZYX, with long grain size, as the donor parent, by multi-generation backcrossing ...
backcrossing, etc ; algorithms; corn; databases; genetic markers; genotype; genotyping; germplasm; heterosis; pedigree; prediction; probability; Show all 12 Subjects
Abstract:
... Along with rapid advances in high-throughput-sequencing technology, the development and application of molecular markers has been critical for the progress that has been made in crop breeding and genetic research. Desirable molecular markers should be able to rapidly genotype tens of thousands of breeding accessions with tens to hundreds of markers. In this study, we developed a multiplex molecula ...
backcrossing, etc ; Callosobruchus; Vigna radiata; agronomy; cultivars; loci; mung beans; phenotypic selection; resistance genes; Show all 9 Subjects
Abstract:
... Mung bean is vulnerable to bruchids (Callosobruchus spp.), resulting in low quality and losses worldwide. Developing resistant cultivars is the most effective, economical, and eco-friendly way to protect mung bean seeds from the damage of bruchids. Previously, we identified two gene loci that are tightly linked with bruchid resistance, which makes new cultivar development possible using molecular ...
backcrossing, etc ; DNA; cell lines; genes; genetic analysis; genetic background; homozygosity; loci; mutants; rice; Show all 10 Subjects
Abstract:
... Genetic analysis on the year-round flowering gene e1, which was derived from Kanto No. 79, an induced mutant by Koshihikari gamma-ray irradiated, was conducted through the backcross process to combine e1 and sd1 in the genetic background of Koshihikari. e1 strongly forwarded flowering 14 days earlier than the original variety Koshihikairi. Isogenic Koshihiakri combining e1 and sd1 was developed by ...
backcrossing, etc ; Bayesian theory; DNA; ancestry; cost effectiveness; dogs; extinction; genes; hybridization; protocols; wolves; Europe; Show all 12 Subjects
Abstract:
... The conservation of pure wolf populations in Europe is currently threatened by anthropogenic hybridization with dogs, through potential extinction of local gene pools due to replacement with domestic gene variants. Distinction of wolf–dog hybrids from wild ancestors is essential for development and implementation of management and conservation plans. Morphological traits are rarely helpful, and a ...
backcrossing, etc ; Acer; Bayesian theory; altitude; climate change; demography; genetic variation; introgression; secondary contact; Taiwan; Show all 10 Subjects
Abstract:
... Hybridization frequently occurs in plant species. With repeated backcross, the introgression may influence evolutionary trajectories through the entry of foreign genes. However, the genetic admixture via hybridization events is often confused with the ancestral polymorphism, especially in closely related species that have experienced similar evolutionary events. In Taiwan, two independent-originat ...
backcrossing, etc ; cataract; chromosomes; hairs; homozygosity; introns; major genes; mice; modifiers (genes); mutation; proteinases; serine; Show all 12 Subjects
Abstract:
... The Nakano cataract mouse (NCT) manifests a wavy coat for their first hair as a genetic trait. In this study, we explored the molecular genetic basis of the wavy coat. We revealed by crossing experiments that the wavy coat is controlled by a major gene on chromosome 7 of NCT, homozygosity of which is a prerequisite for developing the wavy coat, and by a gene on chromosome 9 with a minor effect to ...