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1. Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes

Author:
Coscia, Erin C.; Abutaleb, Nader S.; Hostetter, Bradley; Seleem, Mohamed N.; Breur, Gert J.; McCain, Robyn R.; Crain, Christa J.; Slaby, Ondrej; Capoor, Manu N.; McDowell, Andrew; Ahmed, Fahad S.; Vijayanpillai, Viju; Narayanan, Sanjeev K.; Coscia, Michael F.
Source:
Veterinary sciences 2021 v.8 no.3
ISSN:
2306-7381
Subject:
bacteria,   etc   ; etiology; humans; sheep;     Show all 4 Subjects
Abstract:
... The anaerobic bacterium Cutibacterium acnes has been increasingly linked to the development of degenerative disc disease (DDD), although causality is yet to be conclusively proven. To better study how this organism could contribute to the aetiology of DDD, improved animal models that are more reflective of human disc anatomy, biology and mechanical properties are required. Against this background, ...
DOI:
10.3390/vetsci8030048

2. Detection of Exiguobacterium spp. and E. acetylicum on fresh-cut leafy vegetables by a multiplex PCR assay

Author:
Hu, Antuo; Gao, Cancan; Lu, Zhaoxin; Lu, Fengxia; Kong, Liangyu; Bie, Xiaomei
Source:
Journal of microbiological methods 2021 v.180 pp. 106100
ISSN:
0167-7012
Subject:
bacteria; spoilage bacteria,   etc   ; Exiguobacterium acetylicum; genomics; polymerase chain reaction;     Show all 5 Subjects
Abstract:
... To identify the main spoilage bacterium on fresh-cut leafy vegetables and establish a multiplex PCR assay.Based on physiological-biochemical, molecular identification, and artificial contamination tests, the main bacterium to spoil fresh-cut leafy vegetables was identified as Exiguobacterium spp. and Exiguobacterium acetylicum. Comparative genomics showed that P401_RS0117025 and oxi_50,582,462 gen ...
DOI:
10.1016/j.mimet.2020.106100

3. Bacterial cell recovery after hollow fiber microfiltration sample concentration: Most probable bacterial composition in frozen vegetables

Author:
Irwin, Peter; He, Yiping; Nguyen, Ly; Gehring, Matthew; Gehring, Andrew G.; Chen, Chin-Yi; Capobianco, Joseph
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2021 v.140 pp. 110647
ISSN:
0023-6438
Subject:
bacteria,   etc   ; equipment; microfiltration; temperature; vegetables;     Show all 5 Subjects
Abstract:
... A commercial “concentrating pipette” was scrutinized from the perspective of sample volume reduction to augment native bacterial quantitation and identification within food samples. Aspects related to the elution protocol were evaluated for their impact on bacteria recovery. The results demonstrated there was no significant effect from different diluents/eluents on the relative recovery rate. Furt ...
DOI:
10.1016/j.lwt.2020.110647

4. Phage-based target discovery and its exploitation towards novel antibacterial molecules

Author:
Wan, Xing; Hendrix, Hanne; Skurnik, Mikael; Lavigne, Rob
Source:
Current opinion in biotechnology 2021 v.68 pp. 1-7
ISSN:
0958-1669
Subject:
bacteria,   etc   ; bacteriophages; biotechnology; metabolism; phage therapy;     Show all 5 Subjects
Abstract:
... The deeply intertwined evolutionary history between bacteriophages and bacteria has endowed phages with highly specific mechanisms to hijack bacterial cell metabolism for their propagation. Here, we present a comprehensive, phage-driven strategy to reveal novel antibacterial targets by the exploitation of phage-bacteria interactions. This strategy will enable the design of small molecules, which m ...
DOI:
10.1016/j.copbio.2020.08.015

5. Cyclic Poly(α-peptoid)s by Lithium bis(trimethylsilyl)amide (LiHMDS)-Mediated Ring-Expansion Polymerization: Simple Access to Bioactive Backbones

Author:
Salas-Ambrosio, Pedro; Tronnet, Antoine; Since, Marc; Bourgeade-Delmas, Sandra; Stigliani, Jean-Luc; Vax, Amelie; Lecommandoux, Sébastien; Dupuy, Bruno; Verhaeghe, Pierre; Bonduelle, Colin
Source:
Journal of the American Chemical Society 2021 v.143 no.10 pp. 3697-3702
ISSN:
1520-5126
Subject:
bacteria,   etc   ; Clostridium difficile; cross infection; lithium; polymerization;     Show all 5 Subjects
Abstract:
... Cyclic polymers display unique physicochemical and biological properties. However, their development is often limited by their challenging preparation. In this work, we present a simple route to cyclic poly(α-peptoids) from N-alkylated-N-carboxyanhydrides (NNCA) using LiHMDS promoted ring-expansion polymerization (REP) in DMF. This new method allows the unprecedented use of lysine-like monomers in ...
DOI:
10.1021/jacs.0c13231

6. Functional Amyloids Germinate in Plants

Author:
Santos, Jaime; Ventura, Salvador
Source:
Trends in plant science 2021 v.26 no.1 pp. 7-10
ISSN:
1360-1385
Subject:
bacteria,   etc   ; Archaea; amyloid; fungi; humans; seed longevity;     Show all 6 Subjects
Abstract:
... First identified in human disorders, amyloids serve biological functions in bacteria, archaea, fungi, and animals; however, their role in plants has remained unexplored. Recently, Antonets et al. identified a functional amyloid in plants, with a crucial role in seed longevity, confirming that amyloids are universally exploited for organisms’ adaptation. ...
DOI:
10.1016/j.tplants.2020.10.001

7. Influences of nitrogen forms on abundances and community structures of ammonia and nitrite oxidizers in a slightly alkaline upland soil

Author:
Liu, Xing; Zhang, Ying; Ren, Xiujuan; Shen, Changwei; Wang, Fei; Wu, Dafu
Source:
Archiv für Acker- und Pflanzenbau und Bodenkunde 2021 v.67 no.2 pp. 152-165
ISSN:
1476-3567
Subject:
nitrifying bacteria; nitrogen-fixing bacteria,   etc   ; Archaea; ammonia; community structure; correlation; nitrification; nitrites; nitrogen; oxidants; upland soils;     Show all 11 Subjects
Abstract:
... Nitrogen addition can significantly affect soil nitrification capacity through driving soil nitrifiers. However, the roles of nitrogen forms on nitrifiers are rarely known. This study assessed the effects of nitrogen addition forms (NH₄ ⁺-N and NO₃ –-N) on nitrifiers’ abundances and community structures in a slightly alkaline upland soil by means of a short-term microcosms experiment. Nitrospira-l ...
DOI:
10.1080/03650340.2020.1718113

8. Frozen bovine preputial mucus as a suitable sample for the direct molecular diagnosis of Campylobacter fetus subsp. venerealis

Author:
De Carli, Silvia; Saggin, Bianca; Dias, Maria Eduarda; Lopes, Cassiane Elisabete; Dalto, André Gustavo Cabrera; Rolim, Veronica Machado; Zucco, Renato; Pastre, Giovani; Borges, João Baptista; Siqueira, Franciele Maboni
Source:
Journal of microbiological methods 2020 v.179 pp. 106101
ISSN:
0167-7012
Subject:
bacteria,   etc   ; cattle; mucus; transportation;     Show all 4 Subjects
Abstract:
... Long transportation times remain a challenge to the satisfactory diagnosis of Campylobacter fetus subsp. venerealis (Cfv). Here we demonstrated that samples of frozen bovine preputial mucus maintained at −20 °C for 10 days can be used as an alternative source for molecular diagnosis of Cfv. This approach will improve the analysis of this bacterium. ...
DOI:
10.1016/j.mimet.2020.106101

9. Phagocytosis in a Shape-shifting Bacterium

Author:
Gallot-Lavallée, Lucie; Archibald, John M.
Source:
Trends in microbiology 2020 v.28 no.6 pp. 428-430
ISSN:
0966-842X
Subject:
bacteria,   etc   ; eukaryotic cells; phagocytosis;     Show all 3 Subjects
Abstract:
... Phagocytosis – cell ingestion – is an important process confined to eukaryotes. Or is it? Shiratori et al. have discovered the existence of phagocytosis in a planctomycete bacterium, raising new questions about the significance of phagotrophy beyond the realm of eukaryotic life. ...
DOI:
10.1016/j.tim.2020.02.017

10. Bacterial Aggregation Leads to Collective Elimination

Author:
Kim, Kyeri; You, Lingchong
Source:
Trends in microbiology 2020 v.28 no.4 pp. 243-244
ISSN:
0966-842X
Subject:
bacteria,   etc   ; Danio rerio; antibiotics;     Show all 3 Subjects
Abstract:
... Faster growing bacteria tend to be killed faster by antibiotics. In a complex environment exposed to antibiotics, however, the fate of a bacterial population depends on diverse factors. In a new study, Schlomann et al. describes how sublethal antibiotics can trigger the purging of bacteria by the zebrafish. ...
DOI:
10.1016/j.tim.2019.12.001

11. Phenotypic heterogeneity of microbial populations under nutrient limitation

Author:
Gasperotti, Ana; Brameyer, Sophie; Fabiani, Florian; Jung, Kirsten
Source:
Current opinion in biotechnology 2020 v.62 pp. 160-167
ISSN:
0958-1669
Subject:
bacteria,   etc   ; bioreactors; phenotypic variation;     Show all 3 Subjects
Abstract:
... Phenotypic heterogeneity is a phenomenon in which genetically identical individuals have different characteristics. This behavior can also be found in bacteria, even if they grow as monospecies in well-mixed environments such as bioreactors. Here it is discussed how phenotypic heterogeneity is generated by internal factors and how it is promoted under nutrient-limited growth conditions. A better u ...
DOI:
10.1016/j.copbio.2019.09.016

12. Correction to: Melanin biosynthesis in bacteria, regulation and production perspectives

Author:
Pavan, María Elisa; López, Nancy I.; Pettinari, M. Julia
Source:
Applied microbiology and biotechnology 2020 v.104 no.4 pp. 1821-1822
ISSN:
0175-7598
Subject:
bacteria,   etc   ; biosynthesis; melanin;     Show all 3 Subjects
Abstract:
... The original version of this article contains error for some of the authors corrections were not included during correction stage especially for Table 1. ...
DOI:
10.1007/s00253-019-10331-1

13. The "fighting wisdom and bravery" of tailed phage and host in the process of adsorption

Author:
Ge, Haojie; Hu, Maozhi; Zhao, Ge; Du, Yi; Xu, Nannan; Chen, Xiang; Jiao, Xin’an
Source:
Microbiological research 2020 v.230 pp. 126344
ISSN:
0944-5013
Subject:
bacteria,   etc   ; adsorption; bacteriophages;     Show all 3 Subjects
Abstract:
... In the process of bacteriophage and bacteria struggle, adsorption is the key factor to determine who is the winner. In this paper, the molecular mechanism of tailed bacteriophage recognition and adsorption to host and the strategy of “fighting wisdom and courage” between them are reviewed. ...
DOI:
10.1016/j.micres.2019.126344

14. Management of excessive gingival display using botulinum toxin type A: a descriptive study

Author:
Andriola, Fernando de Oliveira; Chieza, Gabriela Sperotto; Cavagni, Juliano; Freddo, Angelo Luiz; Corsetti, Adriana
Source:
Toxicon 2021 v.196 pp. 56-62
ISSN:
0041-0101
Subject:
bacteria,   etc   ; Clostridium botulinum; botulinum toxin; descriptive studies; muscles; proteinases;     Show all 6 Subjects
Abstract:
... Botulinum toxin is a protease used by the bacterium Clostridium botulinum that causes chemical denervation of skeletal muscles, producing a temporary weakening of muscle activity. Despite having a transitory effect, the application of botulinum toxin has been identified as an alternative for correcting an excessive gingival display (EGD). However, studies evaluating the maintenance of long-term re ...
DOI:
10.1016/j.toxicon.2021.03.016

15. A 'Hydrolase Switch' for Vascular Specialization in Plant Pathogenic Bacteria

Author:
Redkar, Amey; Sabale, Mugdha; Di Pietro, Antonio
Source:
Trends in plant science 2021 v.26 no.5 pp. 427-429
ISSN:
1360-1385
Subject:
plant pathogenic bacteria,   etc   ; Xanthomonas; fungi; genes; hydrolases; pathogenesis;     Show all 6 Subjects
Abstract:
... Plant vascular diseases are tissue-specific systemic infections provoked by bacterial and fungal pathogens adapted to thrive in the xylem vessels. A recent report by Gluck-Thaler et al. reveals that, in the phytopathogenic bacterium Xanthomonas, the switch from non-vascular to vascular pathogenesis is determined by a single gene encoding a plant cell wall-degrading hydrolase. ...
DOI:
10.1016/j.tplants.2021.03.002

16. Plastidial retrograde modulation of light and hormonal signaling: an odyssey

Author:
Jiang, Jishan; Dehesh, Katayoon
Source:
Thenew phytologist 2021 v.230 no.3 pp. 931-937
ISSN:
0028-646X
Subject:
bacteria,   etc   ; biogenesis; chloroplasts; endosymbionts; lifestyle; nuclear genome; photosynthesis;     Show all 7 Subjects
Abstract:
... The transition from an engulfed autonomous unicellular photosynthetic bacterium to a semiautonomous endosymbiont plastid was accompanied by the transfer of genetic material from the endosymbiont to the nuclear genome of the host, followed by the establishment of plastid‐to‐nucleus (retrograde) signaling. The retrograde coordinated activities of the two subcellular genomes ensure chloroplast biogen ...
DOI:
10.1111/nph.17192

17. Manipulation of the Regulatory Genes ppsR and prrA in Rhodobacter sphaeroides Enhances Lycopene Production

Author:
Qu, Yuling; Su, Anping; Li, Ying; Meng, Yonghong; Chen, Zhi
Source:
Journal of agricultural and food chemistry 2021 v.69 no.14 pp. 4134-4143
ISSN:
1520-5118
Subject:
bacteria,   etc   ; Rhodobacter sphaeroides; food chemistry; lycopene; multigene family; oxygen; photosynthesis;     Show all 7 Subjects
Abstract:
... Rhodobacter sphaeroides is a non-sulfur purple bacterium with great metabolic versatility, capable of producing a variety of valuable compounds that include carotenoids and CoQ₁₀. In order to enhance lycopene production, we deleted the photosynthetic gene cluster repressor ppsR from a lycopene-producing Rb. sphaeroides strain (RL1) constructed in a previous study to break the control of carotenoid ...
DOI:
10.1021/acs.jafc.0c08158

18. Phospholipid bilayer responses to ultrasound-induced microbubble cavitation phenomena

Author:
Walsh, Martin P.; Tikekar, Rohan V.; Nitin, Nitin; Wrenn, Steven
Source:
Journal of food engineering 2021 v.294 pp. 110410
ISSN:
0260-8774
Subject:
bacteria,   etc   ; acoustics; electrostatic interactions; energy transfer; mathematical models; microbubbles; phospholipids;     Show all 7 Subjects
Abstract:
... Using liposomal bilayers as a model for bacterial cell membranes, this study investigates the extents to which high-frequency (1.0 and 3.3 MHz) ultrasound-induced microbubble cavitation phenomena are effective for bacterial inactivation. Förster Resonance Energy Transfer (FRET), acoustic scattering and a mathematical model were used to quantify the proximity of microbubbles to liposomes, establish ...
DOI:
10.1016/j.jfoodeng.2020.110410

19. Reprogramming bacteriophage host range: design principles and strategies for engineering receptor binding proteins

Author:
Dunne, Matthew; Prokhorov, Nikolai S; Loessner, Martin J; Leiman, Petr G
Source:
Current opinion in biotechnology 2021 v.68 pp. 272-281
ISSN:
0958-1669
Subject:
bacteria,   etc   ; bacteriophages; biotechnology; directed evolution; host range; tail; therapeutics;     Show all 7 Subjects
Abstract:
... Bacteriophages (phages) use specialized tail machinery to deliver proteins and genetic material into a bacterial cell during infection. Attached at the distal ends of their tails are receptor binding proteins (RBPs) that recognize specific molecules exposed on host bacteria surfaces. Since the therapeutic capacity of naturally occurring phages is often limited by narrow host ranges, there is signi ...
DOI:
10.1016/j.copbio.2021.02.006

20. Fermented Soy Products: Beneficial Potential in Neurodegenerative Diseases

Author:
Jang, Chan Ho; Oh, Jisun; Lim, Ji Sun; Kim, Hyo Jung; Kim, Jong-Sang
Source:
Foods 2021 v.10 no.3
ISSN:
2304-8158
Subject:
Bacillus (bacteria),   etc   ; Aspergillus; Rhizopus; douchi; fermentation; natto; soy sauce; soybeans;     Show all 8 Subjects
Abstract:
... Fermented soybean products, such as cheonggukjang (Japanese natto), doenjang (soy paste), ganjang (soy sauce), and douchi, are widely consumed in East Asian countries and are major sources of bioactive compounds. The fermentation of cooked soybean with bacteria (Bacillus spp.) and fungi (Aspergillus spp. and Rhizopus spp.) produces a variety of novel compounds, most of which possess health benefit ...
DOI:
10.3390/foods10030636