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1. Synthesis of Pleuromutilin

Author:
Nicholas J. Foy; Sergey V. Pronin
Source:
Journal of the American Chemical Society 2022 v.144 no.23 pp. 10174-10179
ISSN:
1520-5126
Subject:
bacterial proteins,   etc   ; cycloaddition reactions; protein synthesis; stereochemistry;     Show all 4 Subjects
Abstract:
... Synthesis of a potent inhibitor of bacterial protein synthesis, pleuromutilin, is described. Assembly of the critical cyclooctane fragment relies on an oxidative ring-expansion, and complete stereochemical relay in the synthetic sequence is enabled by the judicious choice of tactics. The requisite connectivity pattern of the perhydroindanone motif is rapidly established in a sequence of cycloaddit ...
DOI:
10.1021/jacs.2c04708

2. Intermittent induction of LEA peptide by lactose enhances the expression of insecticidal proteins in Bacillus thuringiensis

Author:
Mahmuda Akthar; Tomoko Shimokawa; Yinghan Wu; Taichi Arita; Kazuhiro Mizuta; Yuria Isono; Minoru Maeda; Shinya Ikeno
Source:
FEBS Open Bio 2022 v.12 no.8 pp. 1534-1541
ISSN:
2211-5463
Subject:
bacterial proteins,   etc   ; Bacillus thuringiensis; embryogenesis; lactose; peptides; protein synthesis; recombinant proteins;     Show all 7 Subjects
Abstract:
... Cry toxins from Bacillus thuringiensis (Bt) have been extensively applied in agriculture to substitute the use of chemical insecticides. We have previously reported the use of a coexpression system in which late embryogenesis abundant (LEA) peptides under the control of the lac promoter increase the expression of insecticidal proteins in Bt. The use of lactose to induce the expression of LEA pepti ...
DOI:
10.1002/2211-5463.13448

3. Protein FT-IR amide bands are beneficial to bacterial typing

Author:
Jinghang Xie; Fan Yang; Haimei Shi; Jintao Yan; Hao Shen; Shaoning Yu; Ning Gan; Bin Feng; Li Wang
Source:
International journal of biological macromolecules 2022 v.207 pp. 358-364
ISSN:
0141-8130
Subject:
bacterial proteins,   etc   ; Escherichia coli; Klebsiella pneumoniae; Staphylococcus aureus; nucleic acids; polysaccharides;     Show all 6 Subjects
Abstract:
... Bacterial FT-IR signals are extremely specific and highly reproducible, making FT-IR an efficient tool for bacterial typing at the subspecies level. The polysaccharide and nucleic acid FT-IR regions (1200–900 cm⁻¹) are recommended as a precise and reproducible pattern for bacterial typing. However, proteins are the major macromolecules present in bacteria, and the FT-IR spectral region of proteins ...
DOI:
10.1016/j.ijbiomac.2022.02.161

4. Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates

Author:
Jung-Min Kim; Sung Hee Chung; Inhee Kim; Jae-Seok Kim
Source:
Journal of microbiological methods 2022 v.196 pp. 106471
ISSN:
0167-7012
Subject:
bacterial proteins,   etc   ; agar; culture media; genes; matrix-assisted laser desorption-ionization mass spectrometry; methicillin; methicillin-resistant Staphylococcus aureus; protein synthesis; yeast extract;     Show all 9 Subjects
Abstract:
... Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is routinely used for bacterial identification in clinical laboratories. Bacterial protein expression may differ according to their growth conditions, especially the culture medium composition. We aimed to study the peak variations of Staphylococcus aureus grown on various blood agar plates (BAP), especiall ...
DOI:
10.1016/j.mimet.2022.106471

5. Proteomics reveal the protective effects of chlorogenic acid on Enterococcus faecium Q233 in a simulated pro-oxidant colonic environment

Author:
P. Padilla; M. Estévez; M.J. Andrade; F.J. Peña; J. Delgado
Source:
Food research international 2022 v.157 pp. 111464
ISSN:
0963-9969
Subject:
bacterial proteins,   etc   ; Enterococcus faecium; bacteria; cheeses; chlorogenic acid; energy; food research; hydrogen peroxide; hydrolases; metabolism; oxidative stress; pH; phytochemicals; probiotics; proteome; proteomics;     Show all 16 Subjects
Abstract:
... Certain phytochemicals have been found to promote the beneficial effects of probiotic bacteria although the molecular mechanisms of such interactions are poorly understood. The objective of the present study was to evaluate the impact of the exposure to 0.5 mM chlorogenic acid (CA) on the redox status and proteome of Enterococcus faecium isolated from cheese and challenged with 2.5 mM hydrogen per ...
DOI:
10.1016/j.foodres.2022.111464

6. Trypanosoma cruzi nitroreductase: Structural features and interaction with biological membranes

Author:
Marília L. Cirqueira; Leandro O. Bortot; Maytê Bolean; Mariana A.A. Aleixo; Pedro H. Luccas; Antonio J. Costa-Filho; Ana Paula Ramos; Pietro Ciancaglini; M. Cristina Nonato
Source:
International journal of biological macromolecules 2022 v.221 pp. 891-899
ISSN:
0141-8130
Subject:
bacterial proteins,   etc   ; Chagas disease; Trypanosoma cruzi; benznidazole; cardiolipins; computer simulation; economic impact; geographical distribution; humans; mitochondrial membrane; nitro compounds; nitroreductases; parasites; prediction;     Show all 14 Subjects
Abstract:
... Due to its severe burden and geographic distribution, Chagas disease (CD) has a significant social and economic impact on low-income countries. Benznidazole and nifurtimox are currently the only drugs available for CD. These are prodrugs activated by reducing the nitro group, a reaction catalyzed by nitroreductase type I enzyme from Trypanosoma cruzi (TcNTR), with no homolog in the human host. The ...
DOI:
10.1016/j.ijbiomac.2022.09.073

7. Investigation on Structural Prediction of Pectate Lyase Enzymes from Different Microbes and Comparative Docking Studies with Pectin: The Economical Waste from Food Industry

Author:
Swati Sudeshna Panda; Jyotirmayee Dey; Soumya Ranjan Mahapatra; Gajraj Singh Kushwaha; Namrata Misra; Mrutyunjay Suar; Mrinmoy Ghosh
Source:
Geomicrobiology journal 2022 v.39 no.3-5 pp. 294-305
ISSN:
1521-0529
Subject:
bacterial proteins,   etc   ; Bacillus coagulans; Sphingomonas; computer simulation; energy efficiency; food industry; fruits; hydrophobicity; pectate lyase; pectins; phylogeny; prediction; vegetables; waste treatment; world markets;     Show all 15 Subjects
Abstract:
... Waste from the fruit industry is the main source of pectin, which has a huge global market. Pectinolytic enzymes produced from bacteria have been widely used in the fruit and vegetable processing industry to break down pectic substances. Pectate lyase from mesophilic, thermophilic, and psychrophilic organisms are investigated using in-silico methods, as well as comparative docking with Pectin subs ...
DOI:
10.1080/01490451.2021.1992042

8. Evaluation of a bacterial group 1 LEA protein as an enzyme protectant from stress-induced inactivation

Author:
Enrique Raga-Carbajal; Guadalupe Espin; Marcela Ayala; Julieta Rodríguez-Salazar; Liliana Pardo-López
Source:
Applied microbiology and biotechnology 2022 v.106 no.17 pp. 5551-5562
ISSN:
0175-7598
Subject:
bacterial proteins,   etc   ; Azotobacter vinelandii; bioremediation; biotechnology; catechol; cryoprotectants; dissociation; embryogenesis; freeze-thaw cycles; hydrophilicity; lactate dehydrogenase; microbiology; molecular chaperones; peroxidase; shelf life; stabilizers;     Show all 16 Subjects
Abstract:
... Late embryogenesis abundant (LEA) proteins are hydrophilic proteins that lack a well-ordered tertiary structure and accumulate to high levels in response to water deficit, in organisms such as plants, fungi, and bacteria. The mechanisms proposed to protect cellular structures and enzymes are water replacement, ion sequestering, and membrane stabilization. The activity of some proteins has a limite ...
DOI:
10.1007/s00253-022-12080-0

9. Antibiotics targeting bacterial protein synthesis reduce the lytic activity of bacteriophages

Author:
Medhavi Vashisth; Shikha Yashveer; Taruna Anand; Nitin Virmani; Bidhan Chand Bera; Rajesh Kumar Vaid
Source:
Virus research 2022 v.321 pp. 198909
ISSN:
0168-1702
Subject:
bacterial proteins,   etc   ; Acinetobacter baumannii; Salmonella Typhimurium; Staphylococcus aureus; antagonism; bacteriophages; biosynthesis; cell viability; employment; gentamicin; multiple drug resistance; phage therapy; plate count; protein synthesis; research; tetracycline; turbidity;     Show all 17 Subjects
Abstract:
... Combination therapy of bacteriophages and antibiotics requires careful selection of specific antibiotics as it is crucial towards determining the success of phage therapy to treat multiple drug-resistant bacterial infections. So, we examined how different antibiotics can affect phage lytic activity when used in combination against targeted bacteria. Various antibiotics targeting bacterial protein ...
DOI:
10.1016/j.virusres.2022.198909

10. Radiation synthesis and in vitro evaluation of the antimicrobial property of functionalized nanopolymer-based poly (propargyl alcohol) against multidrug-resistance microbes

Author:
Mohamed Mohamady Ghobashy; Yasser S. Soliman; Mahmoud A.W. Soliman; Gharieb S. El-Sayyad; Atef A. Abdel-Fattah
Source:
Microbial pathogenesis 2022 v.172 pp. 105777
ISSN:
0882-4010
Subject:
bacterial proteins,   etc   ; Candida albicans; Escherichia coli; Staphylococcus aureus; alcohols; antibodies; antimicrobial properties; chloroform; dimethylformamide; gamma radiation; irradiation; minimum inhibitory concentration; multiple drug resistance; nanoparticles; pathogenesis; people; polymers; solvents;     Show all 18 Subjects
Abstract:
... Pathogenic microorganisms are responsible for many diseases in biological organisms, including humans. Many of these infections thrive in hospitals, where people are treated with medicines and certain bacteria resist those treatments. Consequently, this research article aims to develop efficient antimicrobial material-based conjugated and functionalized polypropargyl alcohol nanoparticles (nano-PG ...
DOI:
10.1016/j.micpath.2022.105777

11. The bacterial protein Hfq: Stable modifications and growth phase-dependent changes in SPAM profiles

Author:
Stanley F. Troung; Maxim V. Sukhodolets
Source:
Journal of chromatography 2021 v.1183 pp. 122958
ISSN:
1570-0232
Subject:
bacterial proteins,   etc   ; DNA; Escherichia coli; RNA; arginine; chromatography;     Show all 6 Subjects
Abstract:
... In bacteria transcription is coupled to translation, and while it is broadly accepted that transcription–translation complexes (TTCs) are formed in growing bacterial cells, the exact spatial organization of these macromolecular assemblies is not known with certainty. Recent studies indicated the formation of orderly cytosolic superstructures in growing E. coli cells. The bacterial nucleic acid (NA ...
DOI:
10.1016/j.jchromb.2021.122958

12. Recipes for Inducing Cold Denaturation in an Otherwise Stable Protein

Author:
Angela Bitonti; Rita Puglisi; Massimiliano Meli; Stephen R. Martin; Giorgio Colombo; Piero Andrea Temussi; Annalisa Pastore
Source:
Journal of the American Chemical Society 2022 v.144 no.16 pp. 7198-7207
ISSN:
1520-5126
Subject:
bacterial proteins,   etc   ; cold; denaturation; heat; molecular dynamics; temperature; yeasts;     Show all 7 Subjects
Abstract:
... Although cold denaturation is a fundamental phenomenon common to all proteins, it can only be observed in a handful of cases where it occurs at temperatures above the freezing point of water. Understanding the mechanisms that determine cold denaturation and the rules that permit its observation is an important challenge. A way to approach them is to be able to induce cold denaturation in an otherw ...
DOI:
10.1021/jacs.1c13355

13. Web-Based Protein Interactions Calculator Identifies Likely Proteome Coevolution with Alzheimer’s Disease-Associated Proteins

Author:
Katrisa M. Ward; Brandon D. Pickett; Mark T. W. Ebbert; John S. K. Kauwe; Justin B. Miller
Source:
Genes 2022 v.13 no.8 pp. -
ISSN:
2073-4425
Subject:
bacterial proteins,   etc   ; Internet; algorithms; coevolution; humans; probability; proteome; therapeutics; user interface;     Show all 9 Subjects
Abstract:
... Protein–protein functional interactions arise from either transitory or permanent biomolecular associations and often lead to the coevolution of the interacting residues. Although mutual information has traditionally been used to identify coevolving residues within the same protein, its application between coevolving proteins remains largely uncharacterized. Therefore, we developed the Protein Int ...
DOI:
10.3390/genes13081346

14. Mobility of Bacterial Protein Hfq on dsDNA: Role of C-Terminus-Mediated Transient Binding

Author:
Chuan Jie Tan; Rajib Basak; Indresh Yadav; Jeroen A. van Kan; Véronique Arluison; Johan R. C. van der Maarel
Source:
Journal of physical chemistry 2022 v.126 no.7 pp. 1477-1482
ISSN:
1520-5207
Subject:
bacterial proteins,   etc   ; DNA; amyloid; chromosomes; fluorescence microscopy; metabolism; mutants; physical chemistry;     Show all 8 Subjects
Abstract:
... The mobility of protein is fundamental in the machinery of life. Here, we have investigated the effect of DNA binding in conjunction with DNA segmental fluctuation (internal motion) of the bacterial Hfq master regulator devoid of its amyloid C-terminus domain. Hfq is one of the most abundant nucleoid associated proteins that shape the bacterial chromosome and is involved in several aspects of nucl ...
DOI:
10.1021/acs.jpcb.1c10234

15. Real-time PCR as a decision aid in the control of European foulbrood

Author:
Valérie Grangier; Luc Belloy; Jean-Daniel Charrière; Marcus G Doherr; Albert Fritsche; Andreas S Waldvogel
Source:
Journal of agricultural research 2022 v.54 no.4 pp. 366-372
ISSN:
2078-6913
Subject:
bacterial proteins,   etc   ; Apis mellifera; DNA; European foul brood; Melissococcus plutonius; analytical specificity; apiaries; brood cells; decision support systems; disease control; entomopathogenic bacteria; honey bee colonies; honey bees; microbial load; pathogen identification; quantitative polymerase chain reaction; Switzerland;     Show all 17 Subjects
Abstract:
... The number of cases of European foulbrood (EFB) increased massively in Switzerland since 2000. According to Swiss legislation, the health status of all colonies within one kilometer from a case needs to be identified by a bee inspector. This results in a workload too high to be handled in due time in areas with a high prevalence and high colony density. Therefore, the primary goal of this study wa ...
DOI:
10.1080/00218839.2016.1169650

16. Evaluation of a novel methanotroph bacteria meal grown on natural gas as fish meal substitute in rainbow trout, Oncorhynchus mykiss

Author:
Manchi Rajesh; Biju Sam Kamalam; Prakash Sharma; Vivek Chandra Verma; Anupam Pandey; Maneesh Kumar Dubey; Alexander Ciji; Md. Shahbaz Akhtar; Nityanand Pandey; Debajit Sarma; Sadasivam J. Kaushik
Source:
Aquaculture research 2022 v.53 no.6 pp. 2159-2174
ISSN:
1355-557X
Subject:
bacterial proteins,   etc   ; Oncorhynchus mykiss; alkaline phosphatase; aquaculture; body condition; digestive tract; dry matter digestibility; feed conversion; feed intake; fish meal; histology; ingredients; intestines; kidneys; leucyl aminopeptidase; lipids; liver; methanotrophs; natural gas; nutrient balance; phosphorus; protein efficiency ratio; research;     Show all 23 Subjects
Abstract:
... In the present study, we evaluated the dietary fishmeal (FM) replacement potential of String Pro®, a novel methanotrophic bacterial protein meal (BM), in rainbow trout, based on growth, feed utilization, digestibility, nutrient balance and histological observations. Five isonitrogenous (49.4–51.1%) and isoenergetic (21–22.1 MJ/kg) practical diets containing 0, 12.5, 25, 37.5 and 48% BM, correspond ...
DOI:
10.1111/are.15735

17. Adsorption of urease as part of a complex protein mixture onto soil and its implications for enzymatic activity

Author:
Rayla Pinto Vilar; Kaoru Ikuma
Source:
Biochemical engineering journal 2021 v.171 pp. 108026
ISSN:
1369-703X
Subject:
bacterial proteins,   etc   ; Sporosarcina pasteurii; adsorption; enzyme activity; molecular weight; proteome; proteomics; sand; silt; urease;     Show all 10 Subjects
Abstract:
... Understanding adsorption of important proteins as part of a complex protein mixture onto surfaces such as soil is critical for numerous environmental applications. In this study, we investigated the adsorption of urease as part of a total bacterial protein extract from Sporosarcina pasteurii onto soil mixtures, including sand and silt, and the resulting enzymatic activity. Proteomics analysis of t ...
DOI:
10.1016/j.bej.2021.108026

18. Quantitative proteomic profiling of shake flask versus bioreactor growth reveals distinct responses of Agrobacterium tumefaciens for preparation in molecular pharming

Author:
N. Prudhomme; C. Gianetto-Hill; R. Pastora; W.-F. Cheung; E. Allen-Vercoe; M.D. McLean; D. Cossar; J. Geddes-McAlister
Source:
Canadian journal of microbiology 2021 v.67 no.1 pp. 75-84
ISSN:
1480-3275
Subject:
bacterial proteins,   etc   ; Agrobacterium radiobacter; agroinfiltration; bacterial growth; bioreactors; culture flasks; molecular farming; proteomics; therapeutics;     Show all 9 Subjects
Abstract:
... The preparation of Agrobacterium tumefaciens cultures with strains encoding proteins intended for therapeutic or industrial purposes is an important activity prior to treatment of plants for transient expression of valuable protein products. The rising demand for biologic products such as these underscores the expansion of molecular pharming and warrants the need to produce transformed plants at a ...
DOI:
10.1139/cjm-2020-0238

19. Functional profiling of bacterial communities in Lake Tuz using 16S rRNA gene sequences

Author:
Habeebat Adekilekun Oyewusi; Roswanira Abdul Wahab; Mohamed Faraj Edbeib; Mohd Azrul Naim Mohamad; Azzmer Azzar Abdul Hamid; Yilmaz Kaya; Fahrul Huyop
Source:
Biotechnology & Biotechnological Equipment 2021 v.35 no.1 pp. 1-10
ISSN:
1314-3530
Subject:
bacterial proteins,   etc   ; Clostridium; Fusobacteria; Photobacterium; aquatic environment; bioinformatics; bioremediation; biotechnology; ecosystems; equipment; genes; lakes; metagenomics; saline water;     Show all 14 Subjects
Abstract:
... The 16S rRNA amplicon sequencing technique is a key aspect of studies of microbial communities but does not provide direct evidence of a community’s functional capabilities. This work aimed to assess the structure of the uncultured bacterial communities from two locations in Lake Tuz in Turkey to provide information on their roles in the lake ecosystem. The most abundant phyla in the lake water we ...
DOI:
10.1080/13102818.2020.1840437

20. Magnetotactic Bacteria-Based Biorefinery: Potential for Generating Multiple Products from a Single Fermentation

Author:
Carmen Hierro-Iglesias; Marta Masó-Martínez; Jugraj Dulai; Katie J. Chong; Paula Helena Blanco-Sanchez; Alfred Fernández-Castané
Source:
ACS sustainable chemistry & engineering 2021 v.9 no.31 pp. 10537-10546
ISSN:
2168-0485
Subject:
bacterial proteins,   etc   ; Magnetospirillum gryphiswaldense; bacterial biomass; biorefining; fermentation; magnetotactic bacteria; polyhydroxyalkanoates; pyrolysis; thermal stability; thermogravimetry; volatile compounds;     Show all 11 Subjects
Abstract:
... The magnetotactic bacteria (MTB) model Magnetospirillum gryphiswaldense MSR-1 (Mgryph) is typically known for its capacity to produce magnetic nanoparticles with unique properties, namely, magnetosomes. However, the magnetosome fraction represents only around 4% of the total cell mass. Therefore, the downstream processing of Mgryph generates a substantial amount of under-utilized microbial biomass ...
DOI:
10.1021/acssuschemeng.1c02435