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1. Experimental and theoretical tools to elucidate the binding mechanisms of solid-binding peptides

Author:
Bansal, Rachit; Care, Andrew; Lord, Megan S.; Walsh, Tiffany R.; Sunna, Anwar
Source:
New biotechnology 2019 v.52 pp. 9-18
ISSN:
1871-6784
Subject:
binding capacity,   etc   ; nanobiotechnology; peptides;     Show all 3 Subjects
Abstract:
... The interactions between biomolecules and solid surfaces play an important role in designing new materials and applications which mimic nature. Recently, solid-binding peptides (SBPs) have emerged as potential molecular building blocks in nanobiotechnology. SBPs exhibit high selectivity and binding affinity towards a wide range of inorganic and organic materials. Although these peptides have been ...
DOI:
10.1016/j.nbt.2019.04.001

2. Effects of extreme pH conditions on the stability of As(V)-bearing schwertmannite

Author:
Wang, Yingxu; Gao, Meijuan; Huang, Wanyi; Wang, Tao; Liu, Yuanyuan
Source:
Chemosphere 2020 v.251 pp. 126427
ISSN:
0045-6535
Subject:
binding capacity,   etc   ; arsenic; chemical equilibrium; goethite; iron; pH; sulfates; toxicity;     Show all 8 Subjects
Abstract:
... Schwertmannite (Sch) is known to be an effective scavenger of arsenic (As) due to its strong binding affinity for toxic As species. However, the evolution of As-bearing schwertmannite under extreme pH conditions is poorly understood. In this study, we investigated the effects of extremely acidic and alkaline conditions on the stability of schwertmannite with structurally incorporated As(V) (CO-Sch ...
DOI:
10.1016/j.chemosphere.2020.126427

3. Binding Capacity and Selectivity of Functionalized and Un-functionalized Carbon Nanotubes for Development of Copper-Detecting Printable Sensor

Author:
Peel, Hannah R.; Crouch, Rebecca A.; Martin, David; Stromer, Bobbi S.; Bednar, Anthony J.
Source:
Bulletin of environmental contamination and toxicology 2020 v.104 no.4 pp. 455-463
ISSN:
0007-4861
Subject:
binding capacity,   etc   ; adsorption; carbon nanotubes; copper; models; sorption isotherms;     Show all 6 Subjects
Abstract:
... Carbon nanotubes (CNTs) have unique properties which can be modified through surface functionalization. The ability of several functionalized and un-functionalized CNTs to bind copper was investigated as a first step toward developing a printable CNT-based sensor to detect copper in aqueous systems. Binding capacity and specificity were shown to vary by functionalization and vendor. CNTs from two ...
DOI:
10.1007/s00128-020-02811-2

4. Measuring the affinity of protein-protein interactions on a single-molecule level by mass photometry

Author:
Wu, Di; Piszczek, Grzegorz
Source:
Analytical biochemistry 2020 v.592 pp. 113575
ISSN:
0003-2697
Subject:
binding capacity,   etc   ; binding sites; photometry; protein-protein interactions; stoichiometry;     Show all 5 Subjects
Abstract:
... Measurements of biomolecular interactions are crucial to understand the mechanisms of the biological processes they facilitate. Bulk-based methods such as ITC and SPR provide important information on binding affinities, stoichiometry, and kinetics of interactions. However, the ensemble averaging approaches are not able to probe the intrinsic heterogeneity often displayed by biological systems. Int ...
DOI:
10.1016/j.ab.2020.113575
PubMed:
31923382
PubMed Central:
PMC7069342

5. The endocyclic oxygen atom of d-mannopyranose is involved in its binding to pradimicins

Author:
Watanabe, Yasunori; Yamaji, Fumiya; Ojika, Makoto; Sugawara, Takahiro; Akase, Dai; Aida, Misako; Igarashi, Yasuhiro; Ito, Yukishige; Nakagawa, Yu
Source:
Tetrahedron letters 2020 v.61 no.9 pp. 151530
ISSN:
0040-4039
Subject:
binding capacity,   etc   ; chemical reactions; chemical structure; inositols; molecular models; oxygen;     Show all 6 Subjects
Abstract:
... Methyl 5-thio-α-d-mannopyranoside (1) and six inositols were evaluated for their ability to bind to pradimicins (PRMs) via molecular modeling and three binding assays. In all the experiments, the binding affinity of 1 was slightly lower than that of methyl α-d-mannopyranoside (Man-OMe) and inositols hardly bound to PRMs. These results indicate that the endocyclic oxygen atom of Man-OMe is involved ...
DOI:
10.1016/j.tetlet.2019.151530

6. A ClpS-based N-terminal amino acid binding reagent with improved thermostability and selectivity

Author:
Tullman, Jennifer; Christensen, Makenzie; Kelman, Zvi; Marino, John P.
Source:
Biochemical engineering journal 2020 v.154 pp. 107438
ISSN:
1369-703X
Subject:
binding capacity,   etc   ; Agrobacterium radiobacter; biotechnology; peptides; phenylalanine; thermal stability;     Show all 6 Subjects
Abstract:
... Realization of binding reagents that can perform high-fidelity, sequential recognition and detection of amino acids is important for the success of many proposed approaches for single-molecule protein sequencing. Towards this purpose, a variant of the Agrobacterium tumefaciens protein ClpS was previously engineered with improved binding affinity for phenylalanine at the N-terminus of a peptide. In ...
DOI:
10.1016/j.bej.2019.107438

7. Post-assembly Modification of Phosphine Cages Controls Host–Guest Behavior

Author:
McTernan, Charlie T.; Ronson, Tanya K.; Nitschke, Jonathan R.
Source:
Journal of the American Chemical Society 2019 v.141 no.17 pp. 6837-6842
ISSN:
1520-5126
Subject:
binding capacity,   etc   ; methylation; oxidation; phosphine;     Show all 4 Subjects
Abstract:
... We report the design, synthesis, and post-assembly modification of a new phosphine-paneled supramolecular cage framework, the anion binding ability of which can be modified rationally through selective post-assembly functionalization. The parent phosphine-paneled cage can be modified in situ through oxidation, methylation, or auration. These covalent and coordinative modifications to the exterior ...
DOI:
10.1021/jacs.9b02604

8. Effect of wet and dry milling on the functional properties of whole sorghum grain flour and kafirin

Author:
Amoura, Houria; Mokrane, Hind; Nadjemi, Boubekeur
Source:
Journal of food science and technology 2020 v.57 no.3 pp. 1100-1109
ISSN:
0022-1155
Subject:
binding capacity; water binding capacity,   etc   ; Fourier transform infrared spectroscopy; Sorghum bicolor; dry milling; emulsifying properties; emulsions; flour; foams; gelation; gels; kafirins; landraces; oils; protein content; starch; wet milling;     Show all 17 Subjects
Abstract:
... The effect of wet and dry milling on the functional properties of whole sorghum grain flour (SF) and extracted kafirin were assessed. White sorghum landrace was used to prepare two SFs by wet milling (SF1) or dry milling (SF2) and to extract their respective kafirins SK1 and SK2. Protein contents of SK1 and SK2 were 90.07 and 94.23%. Wet milling of SF allowed increasing the oil binding capacity, t ...
DOI:
10.1007/s13197-019-04145-2
PubMed:
32123431
PubMed Central:
PMC7026331

9. Studies on the interactional characterization of preheated silkworm pupae protein (SPP) with anthocyanins (C3G) and their effect on anthocyanin stability

Author:
Attaribo, Thomas; Jiang, Xizhi; Huang, Gaiqun; Zhang, Bei; Xin, Xiangdong; Zhang, Yueyue; Zhang, Ning; Gui, Zhongzheng
Source:
Food chemistry 2020 v.326 pp. 126904
ISSN:
0308-8146
Subject:
binding capacity,   etc   ; anthocyanins; fluorescence; functional foods; half life; hydrophobic bonding; ingredients; oxidative stability; pupae; silkworms;     Show all 10 Subjects
Abstract:
... In this paper, the interaction of silkworm pupae protein (SPP) with cyanidin-3-O-glucoside (C3G) was studied the protective anthocyanins stability. Characterization experiments suggested that C3G-SPP complexes mainly through hydrophobic interactions, with a decrease in the α-helix content and increases in the β-sheet and β-turn contents. Fluorescence results revealed that C3G quenched the intrinsi ...
DOI:
10.1016/j.foodchem.2020.126904

10. Enhancing the stability of adalimumab by engineering additional glycosylation motifs

Author:
Reslan, Mouhamad; Sifniotis, Vicki; Cruz, Esteban; Sumer-Bayraktar, Zeynep; Cordwell, Stuart; Kayser, Veysel
Source:
International journal of biological macromolecules 2020 v.158 pp. 189-196
ISSN:
0141-8130
Subject:
binding capacity,   etc   ; antigens; engineering; glycosylation; half life; immunogenicity; melting point; monoclonal antibodies; mutation; neoplasms; receptors; therapeutics;     Show all 12 Subjects
Abstract:
... Monoclonal antibodies (mAbs) are of high value in the diagnostic and treatment of many debilitating diseases such as cancers, auto-immune disorders and infections. Unfortunately, protein aggregation is one of the ongoing challenges, limiting the development and application of mAbs as therapeutic products by decreasing half-life, increasing immunogenicity and reducing activity. We engineered an agg ...
DOI:
10.1016/j.ijbiomac.2020.04.147

11. Rational design of porous starch/hyaluronic acid composites for hemostasis

Author:
Wang, Yanling; Liu, Guangwan; Wu, Lijuan; Qu, Hongyuan; Song, Deli; Huang, Hailong; Wu, Changlin; Xu, Min
Source:
International journal of biological macromolecules 2020 v.158 pp. 1319-1329
ISSN:
0141-8130
Subject:
water binding capacity,   etc   ; animal organs; biocompatibility; blood coagulation; commercialization; hemolysis; hemorrhage; hemostasis; hyaluronic acid; sodium; starch;     Show all 11 Subjects
Abstract:
... Effective hemorrhage control is pivotal for decreasing the trauma death both in civilian and military but has proven to be dauntingly challenging, especially for solid viscera and artery trauma. Here we report the fabrication of a novel starch-based hemostat, sodium trimethaphosphate (STMP)-crosslinked starch/hyaluronic acid (HA) (ScSH) porous composites. Aiming at hemostatic potential, physicoche ...
DOI:
10.1016/j.ijbiomac.2020.05.018

12. Enantioselective endocrine-disrupting effects of the phenylpyrazole chiral insecticides in vitro and in silico

Author:
Hu, Kunming; Zhou, Liangliang; Gao, Yingying; Lai, Qi; Shi, Haiyan; Wang, Minghua
Source:
Chemosphere 2020 v.252 pp. 126572
ISSN:
0045-6535
Subject:
binding capacity,   etc   ; computer simulation; ecotoxicology; enantiomers; enantioselectivity; fipronil; insecticides; luciferase; molecular dynamics; simulation models;     Show all 10 Subjects
Abstract:
... The phenylpyrazole chiral insecticides, including the widely used fipronil, ethiprole, and flufiprole, have generated a worldwide interest due to their environmental toxicity. However, up to now,only few studies focused on their their potential endocrine-disrupting effects (EDEs). In this study, we investigated the endocrine hormonal disorder caused by the fipronil, ethiprole, and flufiprole enant ...
DOI:
10.1016/j.chemosphere.2020.126572

13. Thermodynamic and kinetic coupling model of Cd(II) and Pb(II) adsorption and desorption on goethite

Author:
Wu, Jiangtong; Zhao, Xiaopeng; Li, Zipeng; Gu, Xueyuan
Source:
The Science of the total environment 2020 v.727 pp. 138730
ISSN:
0048-9697
Subject:
binding capacity,   etc   ; adsorption; bioavailability; cadmium; desorption; goethite; kinetics; lead; models; pH; soil; sorption isotherms; thermodynamics;     Show all 13 Subjects
Abstract:
... The adsorption and desorption behaviors of Cd(II) and Pb(II) on goethite have important effects on the mobility and bioavailability of these compounds in soils. However, while the respective adsorption isotherms have been extensively investigated, the kinetic process has often been overlooked. Thus, in batch adsorption and stirred-flow experiments this study investigated the thermodynamic and kine ...
DOI:
10.1016/j.scitotenv.2020.138730

14. Affinity shift of ATP upon glycerol binding to a glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1

Author:
Hokao, Ryuta; Matsumura, Hiroyoshi; Katsumi, Ryota; Angkawidjaja, Clement; Takano, Kazufumi; Kanaya, Shigenori; Koga, Yuichi
Source:
Journal of bioscience and bioengineering 2020 v.129 no.6 pp. 657-663
ISSN:
1389-1723
Subject:
binding capacity,   etc   ; Thermococcus kodakarensis; adenosine triphosphate; biosynthesis; crystal structure; crystallization; glycerol; glycerol kinase; glycolysis; lipids; mutants;     Show all 11 Subjects
Abstract:
... Glycerol kinase (GK) is a key enzyme of glycerol metabolism. It participates in glycolysis and lipid membrane biosynthesis. A hexamer of GK from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1(Tk-GK) was identified as a substrate-binding form of the enzyme. Here, the X-ray crystal structure analysis and the biochemical analysis was done and the relationships between its unique oligom ...
DOI:
10.1016/j.jbiosc.2019.12.008

15. Competitive binding assays for measuring the binding affinity of thyroid-disrupting chemicals for integrin αvβ3

Author:
Li, Jian; Liu, Hedan; Zuo, Rui; Yang, Jie; Li, Na
Source:
Chemosphere 2020 v.249 pp. 126034
ISSN:
0045-6535
Subject:
binding capacity,   etc   ; dibutyl phthalate; fluorescence; integrins; ligands; receptors; thyroid function; thyroid hormones;     Show all 8 Subjects
Abstract:
... Some thyroid-disrupting chemicals (TDCs) affect thyroid function by activating the pathways mediated by a typical thyroid hormone (TH) membrane receptor, integrin αᵥβ₃. The present study introduces improved competitive binding assays for the rapid and sensitive evaluation of the binding affinities of TDCs for integrin αᵥβ₃. Based on different probes, two assays were modified: a fluorescence compet ...
DOI:
10.1016/j.chemosphere.2020.126034

16. Incorporation of high intensity ultrasound (HIU) to a scraped surface heat exchanger: Effect of HIU position

Author:
da Silva, Thais Lomonaco Teodoro; Martini, Silvana
Source:
Journal of food engineering 2020 v.274 pp. 109824
ISSN:
0260-8774
Subject:
binding capacity,   etc   ; barrels; crystallization; hardness; heat exchangers; lipid content; microstructure; oils; thermal properties; ultrasonics; viscoelasticity;     Show all 11 Subjects
Abstract:
... The objective of this work was to introduce high-intensity ultrasound (HIU) in different positions within a scraped surface heat exchanger (SSHE) and evaluate the crystallization behavior and physical properties of a low saturated, soybean-based fat. The SSHE in this study was composed of one pump (flow rate of 11 L/h), two crystallization barrels (32 °C, 344 rpm), and one pin worker (208 rpm). HI ...
DOI:
10.1016/j.jfoodeng.2019.109824

17. A novel peptide targeting gastrin releasing peptide receptor for pancreatic neoplasm detection

Author:
Tu, Yuanbiao; Tao, Ji; Wang, Fang; Liu, Peifei; Han, Zhihao; Li, Zhaolun; Ma, Yi; Gu, Yueqing
Source:
Biomaterials science 2020 v.8 no.9 pp. 2682-2693
ISSN:
2047-4849
Subject:
binding capacity,   etc   ; amino acids; dyes; fluorescence; gastrins; image analysis; pancreatic neoplasms; peptide receptors; pharmacokinetics; scintigraphy; technetium;     Show all 11 Subjects
Abstract:
... Pancreatic cancer has a high mortality rate and efforts towards diagnosis and therapy at an early stage are particularly appealing. Recently, a small peptide, BBN₇–₁₄, has attracted much attention for its specific binding ability to gastrin releasing peptide receptor (GRPR), which is highly overexpressed in various types of cancer, including pancreatic cancer. However, its poor stability in vivo r ...
DOI:
10.1039/d0bm00162g

18. Implications from the grafting density and ionic capacity effects on protein adsorption to poly (N,N-dimethylaminopropyl acrylamide)-grafted sepharose FF

Author:
Li, Changsen; Li, Xianxiu; Liu, Yang; Sun, Yan
Source:
Biochemical engineering journal 2020 v.157 pp. 107546
ISSN:
1369-703X
Subject:
binding capacity,   etc   ; acrylamides; adsorption; agarose; chromatography; diffusivity; feedstocks; ionic strength; polymers; resins; salt concentration; sodium chloride;     Show all 12 Subjects
Abstract:
... Our previous study reported a novel protein ion-exchanger, N,N-dimethylaminopropyl acrylamide (DMAPAA)-grafted Sepharose FF, of very high protein adsorption capacity and uptake rate. To gain new insight into the polymer grafting effects on protein adsorption and chromatography, 18 DMAPAA-grafted anion exchangers of four grafting densities and various chain lengths (i.e., ionic capacities, ICs) wer ...
DOI:
10.1016/j.bej.2020.107546

19. Aptamers: an emerging class of bioaffinity ligands in bioactive peptide applications

Author:
Acquah, Caleb; Agyei, Dominic; Obeng, Eugene Marfo; Pan, Sharadwata; Tan, Kei Xian; Danquah, Michael Kobina
Source:
Critical reviews in food science and nutrition 2020 v.60 no.7 pp. 1195-1206
ISSN:
1549-7852
Subject:
binding capacity,   etc   ; amino acids; cost effectiveness; ligands; monitoring; oligonucleotides; peptides; structure-activity relationships;     Show all 8 Subjects
Abstract:
... The food and health applications of bioactive peptides have grown remarkably in the past few decades. Current elucidations have shown that bioactive peptides have unique structural arrangement of amino acids, conferring distinct functionalities, and molecular affinity characteristics. However, whereas interest in the biological potency of bioactive peptides has grown, cost-effective techniques for ...
DOI:
10.1080/10408398.2018.1564234

20. The Role of Evolving Interfacial Substrate Properties on Heterogeneous Cellulose Hydrolysis Kinetics

Author:
Nill, Jennifer Danger; Jeoh, Tina
Source:
ACS sustainable chemistry & engineering 2020 v.8 no.17 pp. 6722-6733
ISSN:
2168-0485
Subject:
binding capacity,   etc   ; binding sites; cellulose; enzymatic hydrolysis; enzyme substrates; enzymes; hydrolysis; models;     Show all 8 Subjects
Abstract:
... Interfacial enzyme reactions require formation of an enzyme–substrate complex at the surface of a heterogeneous substrate, but often multiple modes of enzyme binding and types of binding sites complicate analysis of their kinetics. Excess heterogeneous substrate is often used as a justification to model the substrate as unchanging, but using the study of the enzymatic hydrolysis of insoluble cellu ...
DOI:
10.1021/acssuschemeng.0c00779