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1. Microchip electrophoresis assay for calmodulin binding proteins

Author:
Thushara N. Samarasinghe; Yong Zeng; Carey K. Johnson
Source:
Journal of separation science 2021 v.44 no.4 pp. 895-902
ISSN:
1615-9306
Subject:
calcium; calmodulin; capillary zone electrophoresis; crosslinking; electroosmosis; fluorescence; gel electrophoresis; glass; micellar electrokinetic capillary chromatography; microchip technology; nitric oxide synthase; separation
Abstract:
... The calcium signaling protein calmodulin regulates numerous intracellular processes. We introduce a sensitive microchip assay to separate and detect calmodulin binding proteins. The assay utilizes an optimized microchip electrophoresis protein separation platform with laser‐induced fluorescence detection. Fluorescence‐labeled calmodulin modified with a photoreactive diazirine crosslinker allows ...
DOI:
10.1002/jssc.202000884

2. Biotechnological applications of mammalian odorant-binding proteins

Author:
Filipa Gonçalves; Artur Ribeiro; Carla Silva; Artur Cavaco-Paulo
Source:
Critical reviews in biotechnology 2021 v.41 no.3 pp. 441-455
ISSN:
1549-7801
Subject:
biotechnology; ionic strength; ligands; mammals; mucus; nose; odor compounds; olfactory perception; pH; temperature
Abstract:
... The olfactory system of mammals allows the detection and discrimination of thousands of odors from the environment. In mammals, odorant-binding proteins (OBPs) are considered responsible to carry odorant molecules across the aqueous nasal mucus to the olfactory receptors (ORs). The three-dimensional structure of these proteins presents eight antiparallel β-sheets and a short α-helical segment ...
DOI:
10.1080/07388551.2020.1853672

3. DNA immobilization and detection using DNA binding proteins

Author:
Gülsen Betül Aktas; Arnau Ribera; Vasso Skouridou; Lluis Masip
Source:
Analytical and bioanalytical chemistry 2021 v.413 no.7 pp. 1929-1939
ISSN:
1618-2642
Subject:
adsorption; analytical chemistry; biosensors; oligonucleotides; streptavidin
Abstract:
... The immobilization of sensing bioreceptors is a critical feature affecting the final performance of a biosensor. For DNA detection, the (strept)avidin-biotin affinity interaction is often used for the immobilization of biotin-labeled oligonucleotides or PCR amplicons. Herein, DNA binding proteins are proposed as alternative universal anchors for both DNA immobilization and detection, based ...
DOI:
10.1007/s00216-021-03162-5

4. MHC Class III RNA Binding Proteins and Immunity

Author:
Geraldine Schott; Mariano A. Garcia-Blanco
Source:
RNA biology 2021 v.18 no.5 pp. 640-646
ISSN:
1555-8584
Subject:
RNA; immunity; major histocompatibility complex; monitoring; vertebrates
Abstract:
... Here we review data suggestive of a role for RNA-binding proteins in vertebrate immunity. We focus on the products of genes found in the class III region of the Major Histocompatibility Complex. Six of these genes, DDX39B (aka BAT1), DXO, LSM2, NELFE, PRRC2A (aka BAT2), and SKIV2L, encode RNA-binding proteins with clear roles in post-transcriptional gene regulation and RNA surveillance ...
DOI:
10.1080/15476286.2020.1860388

5. Diversity of phosphoinositide binding proteins in Entamoeba histolytica

Author:
Natsuki Watanabe; Kumiko Nakada-Tsukui; Tomoyoshi Nozaki
Source:
Parasitology international 2021 v.83 pp. 102367
ISSN:
1383-5769
Subject:
Entamoeba histolytica; cell movement; computer simulation; evolution; genome; humans; intestines; organelles; parasites; parasitology; phagocytosis; physiological transport; pleckstrin; protein domains; protein kinases; proteome; surveys
Abstract:
... or an array of PIPs. In Entamoeba histolytica, the human intestinal protozoan parasite, PIPs and PIP-binding proteins have been shown to be involved in their virulence-associated mechanisms such as cell motility, vesicular traffic, trogo- and phagocytosis. In silico search of the domains and the signatures implicated in PIP binding in the E. histolytica proteome allows identification of dozens of potential ...
DOI:
10.1016/j.parint.2021.102367

6. Diabetic endotheliopathy: RNA-binding proteins as new therapeutic targets

Author:
Victoria A. Cornelius; Andrew Yacoub; Sophia Kelaini; Andriana Margariti
Source:
international journal of biochemistry & cell biology 2021 v.131 pp. 105907
ISSN:
1357-2725
Subject:
blood; blood vessels; cardiovascular diseases; diabetes mellitus; endothelial cells; endothelium; gene expression; oxygen; pathogenesis; therapeutics
Abstract:
... expression profile of endothelial cells, with this being largely attributable to the post-transcriptional regulation of RNA-binding proteins. In particular, the RBP Quaking-7 has recently emerged as a crucial mediator of diabetic endotheliopathy, with great potential to become a therapeutic target. ...
DOI:
10.1016/j.biocel.2020.105907

7. The role of solute binding proteins in signal transduction

Author:
Miguel A. Matilla; Álvaro Ortega; Tino Krell
Source:
Computational and Structural Biotechnology Journal 2021 v.19 pp. 1786-1805
ISSN:
2001-0370
Subject:
biotechnology; chemoreceptors; ligands; phosphotransferases (kinases); prokaryotic cells; signal transduction; solutes
Abstract:
... The solute binding proteins (SBPs) of prokaryotes are present in the extracytosolic space. Although their primary function is providing substrates to transporters, SBPs also stimulate different signaling proteins, including chemoreceptors, sensor kinases, diguanylate cyclases/phosphodiesterases and Ser/Thr kinases, thereby causing a wide range of responses. While relatively few such systems have ...
DOI:
10.1016/j.csbj.2021.03.029

8. Identifying Poly(ADP-ribose)-Binding Proteins with Photoaffinity-Based Proteomics

Author:
Morgan Dasovich; Morgan Q. Beckett; Scott Bailey; Shao-En Ong; Marc M. Greenberg; Anthony K. L. Leung
Source:
Journal of the American Chemical Society 2021 v.143 no.8 pp. 3037-3042
ISSN:
1520-5126
Subject:
DNA damage; DNA repair; RNA; breasts; polymers; post-translational modification; proteomics
Abstract:
... synthesized a PAR photoaffinity probe that captures and isolates endogenous PAR binders. Our method identified dozens of known PAR-binding proteins and hundreds of novel candidates involved in DNA repair, RNA processing, and metabolism. PAR binding by eight candidates was confirmed using pull-down and/or electrophoretic mobility shift assays. Using PAR probes of defined lengths, we detected proteins ...
DOI:
10.1021/jacs.0c12246

9. Regulation and function of class A Penicillin-binding proteins

Author:
Manuel Pazos; Waldemar Vollmer
Source:
Current opinion in microbiology 2021 v.60 pp. 80-87
ISSN:
1369-5274
Subject:
cell division; cell growth; cell membranes; peptides; peptidoglycans; turgor
Abstract:
... Most bacteria surround their cell membrane with a peptidoglycan sacculus that counteracts the turgor and maintains the shape of the cell. Class A PBPs are bi-functional glycosyltransferase-transpeptidases that polymerize glycan chains and cross-link peptides. They have a major contribution to the total peptidoglycan synthesized during cell growth and cell division. In recent years it became appare ...
DOI:
10.1016/j.mib.2021.01.008

10. RNA-Binding Proteins Hold Key Roles in Function, Dysfunction, and Disease

Author:
Sophia Kelaini; Celine Chan; Victoria A Cornelius; Andriana Margariti
Source:
Biology 2021 v.10 no.5 pp. -
ISSN:
2079-7737
Subject:
RNA; cardiovascular diseases; diabetes; homeostasis; metabolism; pathogenesis; stress response
Abstract:
... RNA-binding proteins (RBPs) are multi-faceted proteins in the regulation of RNA or its RNA splicing, localisation, stability, and translation. Amassing proof from many recent and dedicated studies reinforces the perception of RBPs exerting control through differing expression levels, cellular localization and post-transcriptional alterations. However, since the regulation of RBPs is reliant ...
DOI:
10.3390/biology10050366

11. Non-canonical roles of canonical telomere binding proteins in cancers

Author:
Semih Can Akincilar; Claire Hian Tzer Chan; Qin Feng Ng; Kerem Fidan; Vinay Tergaonkar
Source:
Cellular and molecular life sciences 2021 v.78 no.9 pp. 4235-4257
ISSN:
1420-682X
Subject:
carcinogenesis; cytotoxicity; telomerase; telomeres; therapeutics
Abstract:
... Reactivation of telomerase is a major hallmark observed in 90% of all cancers. Yet paradoxically, enhanced telomerase activity does not correlate with telomere length and cancers often possess short telomeres; suggestive of supplementary non-canonical roles that telomerase might play in the development of cancer. Moreover, studies have shown that aberrant expression of shelterin proteins coupled w ...
DOI:
10.1007/s00018-021-03783-0

12. Revisiting poly(A)‐binding proteins: Multifaceted regulators during gametogenesis and early embryogenesis

Author:
Long‐Wen Zhao; Heng‐Yu Fan
Source:
BioEssays 2021 v.43 no.6 pp. e2000335
ISSN:
0265-9247
Subject:
catalytic activity; chromatin; embryogenesis; gametogenesis; gene expression; human fertility; metabolism; tail
Abstract:
... ‐poly(A) tail is covered with poly(A)‐binding proteins (PABPs) that help to mediate mRNA metabolism and recent work has shed light on the number and function of germ cell‐specific expressed PABPs. There are two structurally different PABP groups distinguished by their cytoplasmic and nuclear localization. Both lack catalytic activity but are coupled with various roles through their interaction ...
DOI:
10.1002/bies.202000335

13. The La-related proteins: structures and interactions of a versatile superfamily of RNA-binding proteins

Author:
Anne-Catherine Dock-Bregeon; Karen A. Lewis; Maria R. Conte
Source:
RNA biology 2021 v.18 no.2 pp. 178-193
ISSN:
1555-8584
Subject:
RNA-binding proteins,   etc   ; RNA; protein-protein interactions; structure-activity relationships; webs;     Show all 5 Subjects
Abstract:
... The La-related proteins (LaRPs) are an ancient superfamily of RNA-binding proteins orchestrating the major fates of RNA, from processing and maturation to regulation of mRNA translation. LaRPs are instrumental in modulating complex assemblies where the RNA is bound, folded, processed, escorted and presented to the functional effectors often through recruitment of protein partners. This intricate ...
DOI:
10.1080/15476286.2019.1695712

14. The Interplay of RNA Binding Proteins, Oxidative Stress and Mitochondrial Dysfunction in ALS

Author:
Jasmine Harley; Benjamin E. Clarke; Rickie Patani
Source:
Antioxidants 2021 v.10 no.4 pp. -
ISSN:
2076-3921
Subject:
RNA; amyotrophic lateral sclerosis; gene expression; mitochondria; oxidative stress; therapeutics
Abstract:
... RNA binding proteins fulfil a wide number of roles in gene expression. Multiple mechanisms of RNA binding protein dysregulation have been implicated in the pathomechanisms of several neurodegenerative diseases including amyotrophic lateral sclerosis (ALS). Oxidative stress and mitochondrial dysfunction also play important roles in these diseases. In this review, we highlight the mechanistic ...
DOI:
10.3390/antiox10040552

15. Reprogramming bacteriophage host range: design principles and strategies for engineering receptor binding proteins

Author:
Matthew Dunne; Nikolai S Prokhorov; Martin J Loessner; Petr G Leiman
Source:
Current opinion in biotechnology 2021 v.68 pp. 272-281
ISSN:
0958-1669
Subject:
bacteria; bacteriophages; biotechnology; directed evolution; host range; tail; therapeutics
Abstract:
... Bacteriophages (phages) use specialized tail machinery to deliver proteins and genetic material into a bacterial cell during infection. Attached at the distal ends of their tails are receptor binding proteins (RBPs) that recognize specific molecules exposed on host bacteria surfaces. Since the therapeutic capacity of naturally occurring phages is often limited by narrow host ranges ...
DOI:
10.1016/j.copbio.2021.02.006

16. Transient non-specific DNA binding dominates the target search of bacterial DNA-binding proteins

Author:
Mathew Stracy; Jakob Schweizer; David J. Sherratt; Achillefs N. Kapanidis; Stephan Uphoff; Christian Lesterlin
Source:
Molecular cell 2021 v.81 no.7 pp. 1499-1514.e6
ISSN:
1097-2765
Subject:
DNA; Escherichia coli; chromosomes
Abstract:
... Despite their diverse biochemical characteristics and functions, all DNA-binding proteins share the ability to accurately locate their target sites among the vast excess of non-target DNA. Toward identifying universal mechanisms of the target search, we used single-molecule tracking of 11 diverse DNA-binding proteins in living Escherichia coli. The mobility of these proteins during the target ...
DOI:
10.1016/j.molcel.2021.01.039

17. Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Author:
Haixia Zhan; Du Li; Youssef Dewer; Changying Niu; Fengqi Li; Chen Luo
Source:
Heliyon 2021 v.7 no.3 pp. e06427
ISSN:
2405-8440
Subject:
Drosophila melanogaster; Drosophila suzukii; acetates; berberine; chlorides; coumarin; courtship; fruit flies; fruits; ligands; males; odor compounds; odorant receptors; plant pests; sex pheromones; smell; summer; taste
Abstract:
... ) is still functional. The lack of cVA in D. suzukii casts the question of whether this pheromone might have been replaced by another compound similar to cVA that disrupts mating in D. suzukii. In order to address this question, we cloned two D. suzukii adult antenna-specific odorant-binding proteins (OBPs) DsOBP69a and DsOBP76a and aligned with their D. melanogaster orthologues. Moreover, we examined ...
DOI:
10.1016/j.heliyon.2021.e06427

18. Qualitative and semiquantitative assessment of thyroid hormone binding proteins in greyhounds and other dog breeds

Author:
R.E. Shiel; C.M. Nolan; J.E. Nally; K.R. Refsal; C.T. Mooney
Source:
Domestic animal endocrinology 2021 v.76 pp. 106623
ISSN:
0739-7240
Subject:
Greyhound; blood serum; dogs; domestic animals; electrophoresis; gels; globulins; humans; liver; prealbumin; reverse transcriptase polymerase chain reaction; thyroid hormones; thyroxine
Abstract:
... Total thyroxine (T4) concentrations are lower in healthy greyhounds compared to most other non-sighthound breeds. In humans, variations in the structure or concentration of the major thyroid hormone binding proteins are responsible for most reported differences between total T4 concentrations in healthy individuals from different ethnic groups or other subpopulations. The aim of this study ...
DOI:
10.1016/j.domaniend.2021.106623

19. A distinctive pathway for triplet-triplet energy transfer photoprotection in fucoxanthin chlorophyll-binding proteins from Cyclotella meneghiniana

Author:
Alessandro Agostini; Claudia Büchel; Marilena Di Valentin; Donatella Carbonera
Source:
Biochimica et biophysica acta 2021 v.1862 no.1 pp. 148310
ISSN:
0005-2728
Subject:
chlorophyll binding proteins,   etc   ; Bacillariophyceae; Cyclotella meneghiniana; carotenoids; electron paramagnetic resonance spectroscopy; energy transfer; exhibitions; magnetism; microalgae; radiation resistance; spectral analysis;     Show all 11 Subjects
Abstract:
... Fucoxanthin chlorophyll-binding proteins (FCPs) are the major light-harvesting complexes of diatoms. In this work, FCPs isolated from Cyclotella meneghiniana have been studied by means of optically detected magnetic resonance (ODMR) and time-resolved electron paramagnetic resonance (TR-EPR), with the aim to characterize the photoprotective mechanism based on triplet–triplet energy transfer (TTET ...
DOI:
10.1016/j.bbabio.2020.148310

20. Determination of the iron bioavailability, conformation, and rheology of iron‐binding proteins from Tegillarca granosa

Author:
Gaoshang Li; Junqi Zhan; Anqi Xu; Beibei Tan; Nan Sun; Chen Wang; Ru Jia; Chao Li; Jinjie Zhang; Wenge Yang
Source:
Journal of food biochemistry 2021 v.45 no.1 pp. e13517
ISSN:
0145-8884
Subject:
Tegillarca granosa; bioavailability; cattle; diet; digestion; ferritin; food production; functional foods; hemoglobin; human nutrition; iron; iron absorption; models; rheology
Abstract:
... The increased interest in achieving, solely through diet, the same effect on iron levels with supplementation, leads to numerous studies on iron absorption of iron binding proteins (IBPs). The characteristics of IBPs from Tegillarca granosa (T. granosa) and its iron utilization were determined to analyze their relationship. The results showed in T. granosa, Fe(ӀӀ) was main iron form in hemoglobin ...
DOI:
10.1111/jfbc.13517