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... Nowadays, the application of rapid molecular-based methods such as PCR/qPCR and isothermal techniques like Recombinase Polymerase Amplification (RPA), has increased in order to overcome some of the drawbacks of traditional culture-based methods due to their high sensitivity and specificity. One of the main limitations of these techniques is their inability to differentiate between live and dead mi ...
Elvira de Lourdes Chaves Macêdo; Tatiana Colombo Pimentel; Dirceu de Sousa Melo; Angélica Cristina de Souza; Janne Santos de Morais; Marcos dos Santos Lima; Disney Ribeiro Dias; Rosane Freitas Schwan; Marciane Magnani
... Caatinga Biome fruits have been scarcely explored as a source of biotechnological yeasts. This study isolated yeasts from naturally fermented Caatinga fruits and evaluated Hanseniaspora opuntiae125,Issatchenkia terricola 129, and Hanseniaspora opuntiae 148 on fermentation of soursop and umbu-cajá pulps. All strains were able to ferment the pulps (72 h), increasing (p < 0.05) acetic acid, phenolics ...
biochemicalpathways, etc ; acidification; aeration; ammonia; ammonification; ammonium nitrogen; biodegradation; cathodes; chemical oxygen demand; demethylation; fermentation; hydrolysis; metagenomics; microbial communities; nitrification; oxygen; toxicology; wastewater; Show all 18 Subjects
Abstract:
... Recalcitrant and toxicological membrane-making wastewater displays negative impacts on environment, and this is difficult to treat efficiently using conventional hydrolytic acidification. In this study, a novel electro-assisted biological reactor with micro-aerobic cathode (EABR-MAC) was developed to improve the biodegradation and ammonification of N, N-dimethylformamide (DMF) in membrane-making w ...
biochemicalpathways, etc ; Danio rerio; bacterial contamination; cell membranes; death; magnetism; models; nanocomposites; nanomaterials; oxidative stress; photocatalysis; photocatalysts; proteomics; seawater; solar radiation; toxicity; water treatment; Show all 17 Subjects
Abstract:
... In recent years, many endeavours have been prompted with photocatalytic nanomaterials by the need to eradicate pathogenic microorganisms from water bodies. Herein, a tocopherol-assisted Ag-Fe₃O₄-TiO₂ nanocomposite (TAFTN) was synthesized for photocatalytic bacterial inactivation. The prepared TAFTN became active under sunlight due to its narrowed bandgap, inactivating the bacterial contaminants vi ...
... Salmonella spp. is one of the leading causes of foodborne outbreaks worldwide. Salmonella spp. has been associated with a variety of food sources, particularly egg products. They can enter a viable but nonculturable (VBNC) state in response to harsh stress. VBNC cells still retain membrane integrity and metabolic activity, which may pose health risks. However, the formation mechanism and resuscita ...
... This study was to investigate the structure and rat fecal microbial fermentation properties of a polysaccharide fraction (PHP2) isolated from the red marine alga Porphyra haitanensis. PHP2 was characterized as a sulfated glucogalactan, with a hypothetical backbone structure of →4)Gα(1→6)G4Sβ(1→4)Glc(1→ and a side chain of Man(1→6)Glc. PHP2 had an irregular spherical chain conformation. The 16S rRN ...
... Due to the public health concern of arsenic, environmental management measures in mining areas had been implemented. To assess the effect of environmental management measures in the mining area comprehensively, arsenic accumulation in the urine, hair, nails, and urinary metabolites of residents in a realgar mining area in Hunan province, China were investigated in 2019, and the changes in arsenic ...
... Simultaneous removal of ammonium and nitrate was achieved in a methane-fed moving bed biofilm reactor (MBBR). In the reactor, methanotrophic microorganisms oxidized methane under hypoxic conditions likely to methanol, hence providing an electron donor to denitrifiers to reduce nitrate to nitrite that then allowed anaerobic ammonium oxidizing bacteria (Anammox) to remove excess ammonium as N₂. The ...
... Direct discharge of aquaculture wastewater may have toxic effects, due to the presence of heavy metals, antibiotics, and even resistant pathogens, but little attention has been given. Here, tanks simulating a wild ecosystem were built to study the effects of long-term exposure to duck wastewater containing oxytetracycline (OTC) and/or arsenic (As) on the growth, physiological function, and gut mic ...
biochemicalpathways, etc ; DNA barcoding; Zea mays; agroecosystems; ammonium; biodegradability; bioplastics; community structure; corn; dose response; metabolites; microbial activity; microbial communities; microplastics; nitrates; nitrogen; plant growth; soil; soil quality; Show all 19 Subjects
Abstract:
... Microplastic contamination poses a significant threat to agroecosystem functioning, provoking a move away from the use of conventional oil-based plastics in agriculture, to biodegradable alternatives that may be degraded over a shorter timescale. The impact of these bioplastics on plant and soil health, however, has received relatively little attention. Here, we investigated the effect of soil loa ...
... Persimmon fruits accumulate proanthocyanidins during development. Proanthocyanidins form insoluble complexes with saliva proteins, which promote a sensation of dryness in the mouth called astringency. Persimmon cultivars are classified according to pollination and the presence of astringency at harvest. The different characteristics between persimmon cultivars lead to different metabolic pathways ...
biochemicalpathways, etc ; biosynthesis; biotechnology; genome; Show all 4 Subjects
Abstract:
... Non-model microorganisms possess unique and versatile metabolic characteristics, offering great opportunities as cell factories for biosynthesis of target products. However, lack of efficient genetic tools for pathway engineering represents a big challenge to unlock the full production potential of these microbes. Over the past years, CRISPR systems have been extensively developed and applied to d ...
biochemicalpathways, etc ; biotechnology; enzymatic reactions; Show all 3 Subjects
Abstract:
... The metabolic engineering community relies on computational methods for pathway design to produce important small molecules in microbial hosts. Metabolic network databases are continuously curated and updated with known and novel reactions that expand the known biochemistry based on different sets of enzymatic reaction rules. To address the complexity of the metabolic networks, elaborate methods w ...
biochemicalpathways, etc ; analytical chemistry; cytoskeleton; Show all 3 Subjects
Abstract:
... Supramolecular chemistry is the quintessential backbone of all biological processes. It encompasses a wide range from the metabolic network to the self-assembled cytoskeletal network. Combining the chemical diversity with the plethora of functional depth that biological systems possess is a daunting task for synthetic chemists to emulate. The only route for approaching such a challenge lies in und ...
biochemicalpathways, etc ; antigen presentation; computer simulation; riboflavin; Show all 4 Subjects
Abstract:
... Mucosal-associated invariant T (MAIT) cells are innate T cells recognising intermediates of the vitamin B2 biosynthetic pathway presented by the monomorphic MR1 molecule. We have demonstrated that in addition to their cytolytic activity important in antimicrobial defence, MAIT cells have also immune-modulatory functions, enhancing DC maturation, in vitro and in vivo. Furthermore, through an in sil ...
biochemicalpathways, etc ; Plasmodium falciparum; antimalarials; chemical reactions; Show all 4 Subjects
Abstract:
... A bioinspired strategy is described for the total synthesis of spiroaxillarone A, which exhibited significant antimalarial activity against resistant Plasmodium falciparum (IC₅₀ = 2.32 μM). The key steps include an intermolecular ethanethiol Michael addition, o-quinone Michael addition, and subsequent β-ethanethiol elimination. This synthetic sequence provides a potential biosynthetic pathway of s ...
biochemicalpathways, etc ; Tardigrada; anhydrobiosis; humans; life history; models; Show all 6 Subjects
Abstract:
... Anhydrobiosis is induced by loss of water and indicates dehydration tolerance. Survival of dehydration is possible through changes at different levels of organism organization, including a remarkable reduction in metabolic activity at the cellular level. Thus, anhydrobiosis may be regarded as an anti-aging strategy. Accordingly, two hypotheses named after popular stories, “Sleeping Beauty” and “Th ...
Jia-Xuan Yan; Qihao Wu; Eric J. N. Helfrich; Marc G. Chevrette; Doug R. Braun; Heino Heyman; Gene E. Ananiev; Scott R. Rajski; Cameron R. Currie; Jon Clardy; Tim S. Bugni
biochemicalpathways, etc ; Bacillus (bacteria); antibacterial properties; bioinformatics; glycolysis; Show all 5 Subjects
Abstract:
... Chemical investigations of a marine sponge-associated Bacillus revealed six new imidazolium-containing compounds, bacillimidazoles A–F (1–6). Previous reports of related imidazolium-containing natural products are rare. Initially unveiled by timsTOF (trapped ion mobility spectrometry) MS data, extensive HRMS and 1D and 2D NMR analyses enabled the structural elucidation of 1–6. In addition, a plaus ...
biochemicalpathways, etc ; Podophyllum peltatum; biosynthesis; podophyllotoxin; supply balance; Show all 5 Subjects
Abstract:
... Podophyllotoxin (PTOX, 1), a kind of aryltetralin-type lignan, was first discovered in the plant Podophyllum peltatum and its structure was clarified by W. Borsche and J. Niemann in 1932. Due to its potent anti-cancer and anti-viral activities, it is considered one of the molecules most likely to be developed into modern drugs. With the increasing market demand and insufficient storage of natural ...
Niraj Aryal; Junhong Chen; Keshab Bhattarai; Oliver Hennrich; Ira Handayani; Markus Kramer; Jan Straetener; Tatjana Wommer; Anne Berscheid; Silke Peter; Norbert Reiling; Heike Brötz-Oesterhelt; Christian Geibel; Michael Lämmerhofer; Yvonne Mast; Harald Gross
biochemicalpathways, etc ; Streptomyces; cytotoxicity; enantioselectivity; plasticity; pyrimidine nucleosides; Show all 6 Subjects
Abstract:
... A chemical reinvestigation of the Indonesian strain Streptomyces sp. SHP 22-7 led to the isolation of three new pyrimidine nucleosides, along with six known analogues and zincphyrin. The structures of the new compounds (6, 7, 10) were elucidated by employing spectroscopic techniques (NMR, MS, CD, and IR) as well as enantioselective analyses of methyl branched side chain configurations. Application ...