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bioluminescence; bioluminescence assay, etc ; analytical chemistry; biochemical pathways; bioimaging; biosensors; cancer therapy; cell viability; chemical reactions; genes; luciferase; stem cells; toxicity; Show all 13 Subjects
Abstract:
... Bioluminescence occurs through a chemical reaction in organisms that spontaneously produce light. Luminescent bacteria are unique among bioluminescent organisms. Their bioluminescence intensity is an indicator of their metabolic activity, which can directly reflect the influence of environmental factors on cell viability. Moreover, the whole bioluminescence process is totally gene encoded without ...
bioluminescence, etc ; bioimaging; calcium; endoplasmic reticulum; fluorescence; rats; Show all 6 Subjects
Abstract:
... We introduce a family of bright, rhodamine-based calcium indicators with tuneable affinities and colors. The indicators can be specifically localized to different cellular compartments and are compatible with both fluorescence and bioluminescence readouts through conjugation to HaloTag fusion proteins. Importantly, their increase in fluorescence upon localization enables no-wash live-cell imaging, ...
bioluminescence, etc ; Sergestidae; genomics; mitochondria; mitochondrial genome; monophyly; shrimp; Show all 7 Subjects
Abstract:
... Deep-sea shrimp of the family Sergestidae Dana, 1852 provide a unique system for studying the evolution of bioluminescence. Most species within the family possess autogenic bioluminescent photophores in one of three distinct forms: lensed photophores; non-lensed photophores; or internal organs of Pesta. This morphological diversity across the Sergestidae has resulted in recent major taxonomic revi ...
bioluminescence assay, etc ; air pollution; ammonia; autophagy; bioinformatics; humans; jejunum; Show all 7 Subjects
Abstract:
... With the continued increase of global ammonia emission, the damage to human or animal caused by ammonia pollution has attracted wide attention. The noncoding RNAs have been reported to regulate a variety of biological processes under different environmental stimulation via ceRNA (competing endogenous RNA) networks. Autophagy is a hallmark of tissue damage from air pollution. However, the specific ...
bioluminescence, etc ; Lemna; Wolffiella; circadian clocks; circadian rhythm; environment; phylogeny; Show all 7 Subjects
Abstract:
... The circadian clock system is widely conserved in plants; however, divergence in circadian rhythm properties is poorly understood. We conducted a comparative analysis of the circadian properties of closely related duckweed species. Using a particle bombardment method, a circadian bioluminescent reporter was introduced into duckweed plants. We measured bioluminescence circadian rhythms of eight spe ...
bioluminescence; bioluminescence assay, etc ; biofilm; dopamine; polymerization; sodium; ultrafiltration; Show all 7 Subjects
Abstract:
... Pure-water filtration membranes are often fouled by bacterial biofilms. Antibacterial coatings for preventing biofilm formation on such membranes should not rely on leaching of inhibiting compounds but should only be effective on surface contact. Certified assays for antibacterial coatings do not sufficiently exclude leaching effects and involve nutrient-rich cultivation media that do not correspo ...
bioluminescence, etc ; Vibrio fischeri; cations; ecotoxicology; electrochemistry; lithium; pollution; research; toxicity; toxicity testing; Show all 10 Subjects
Abstract:
... The applicability of ionic liquids (ILs) has increased over the last years, and even new opportunities are becoming a reality, i.e. mixtures of pure IL and inorganic salt as electrolytes for smart electrochemical devices, yet the effects on the environment are almost unknown. In this work, the ecotoxicity of two pure protic ILs (Ethylammonium nitrate and Ethylimidazolium nitrate) and two pure apro ...
bioluminescence assay, etc ; Clostridium perfringens; Dachshund; apoptosis; diarrhea; ileum; immunology; inflammation; toxins; virulence; Show all 10 Subjects
Abstract:
... Clostridium perfringens (C. perfringens) type C (CPC) is one of the chief pathogens that causes diarrhea in piglets, and C. perfringens beta2 (CPB2) toxin is the main virulence factor of CPC. Our previous research demonstrated that ssc-microR-132 was differentially expressed in ileal tissues of CPC-mediated diarrheic piglets and healthy piglets, which implied a potential role of ssc-microR-132 in ...
bioluminescence, etc ; Leishmania infantum; agar; antiparasitic agents; chromatography; genes; leishmaniasis; luciferase; macrophages; parasites; subtropics; therapeutics; Show all 12 Subjects
Abstract:
... Leishmania genus is responsible for leishmaniasis, a group of diseases affecting 12 million people in the tropical and subtropical zone. Currently, the few drugs that are available to treat this disease are expensive and cause many side effects. Searching for new therapeutics from plant species seems to be a promising path. This work proposes an original HPTLC test against parasites, in particular ...
... Biofluorescence has been observed in a variety of fishes, but is rare in deep‐sea environments where light from the surface cannot reach. Here, we document biofluorescence in an oceanic anglerfish, the Pacific footballfish. Green biofluorescence was observed in small spots on the distal surface of the esca. While the wavelength of bioluminescent light is unknown for this species, it is possible th ...
bioluminescence, etc ; Lampyridae; fluorescence; physiology; Show all 4 Subjects
Abstract:
... Fluorescence across the family Lampyridae has been documented sporadically but not comprehensively in formal research. Fireflies (Coleoptera: Lampyridae), best known for their bioluminescence, are also fluorescent. This fluorescence has been documented in several genera within the clade but is still an often overlooked aspect of firefly physiology in the common understanding of the species. To thi ...
bioluminescence, etc ; adverse outcome pathways; chemical risk assessment; computer simulation; dimerization; energy transfer; estrogen receptors; humans; prediction; Show all 9 Subjects
Abstract:
... The adverse outcome pathway (AOP) has been recently proposed as an effective framework for chemical risk assessment. The AOP framework offers the advantage of effectively integrating individual in vitro studies and in silico prediction models. Thus, the development of an effective testing method to measure key events caused by chemicals is essential for chemical risk assessment through a fully dev ...
... Sunflower production plays an important role in global industrial oil production. Two-spotted spider mite, Tetranychus urticae, is a dangerous, polyphagous arthropod pest with a cosmopolitan distribution, which can endanger the economic features of global sunflower production. It is widely known that pests trigger various reactions in host plants. Among these, detection of ultra-weak photon emissi ...
bioluminescence, etc ; analytical chemistry; cytotoxicity; drugs; energy transfer; fluorescence; fluorescence emission spectroscopy; fluorescent dyes; ligands; moieties; pharmacology; Show all 11 Subjects
Abstract:
... By fusing several environment-sensitive fluorophores to the pharmacophore mirabegron, a series of new fluorescent ligands for β-adrenergic receptors (β-ARs) were produced with a turn-on mechanism and high binding affinity to β-ARs efficiently. Compound L5 with the pyridinium moiety possessed the most favorable combination of properties after systematic comparison and optimization, including high a ...
bioluminescence, etc ; enzyme substrates; luciferase; mutants; Show all 4 Subjects
Abstract:
... Bioluminescence imaging with luciferase–luciferin pairs is routinely used to monitor cellular functions. Multiple targets can be visualized in tandem using luciferases that process unique substrates, but only a handful of such orthogonal probes are known. Multiplexed studies require additional robust, light-emitting molecules. In this work, we report new luciferins for orthogonal imaging that comp ...
bioluminescence, etc ; DNA; Hepatitis B virus; Hepatitis C virus; agar gel electrophoresis; biotechnology; blood serum; detection limit; instrumentation; Show all 9 Subjects
Abstract:
... The transmission of bloodborne viruses through transfusion remains a major blood supply–related safety concern, with hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV) being the most important pathogens in this context. Real-time bioluminescent pyrophosphate testing has been developed as a means of readily detecting bacterial cells within particular sample typ ...
bioluminescence assay, etc ; genes; growth and development; horticultural crops; nitrate nitrogen; nitrates; nitrogen; nutrient use efficiency; phylogeny; watermelons; Show all 10 Subjects
Abstract:
... To balance the relationship between high yield and low nitrogen supply, the nitrogen utilization efficiency of watermelon needs to be improved urgently. Nodule inception-like Protein (NLP) transcription factors play a key node role in nitrate response and growth and development of plant, however, comprehensive analysis of the NLP gene family in watermelon is unclear. This study explored the functi ...
bioluminescence assay, etc ; Citrus; Western blotting; colorimetry; flavanones; melanin; melanogenesis; melanoma; myosin; phosphorylation; polymethoxyflavones; silver; transcription factors; transportation; Show all 14 Subjects
Abstract:
... 5-demethylnobiletin is a natural polymethoxyflavone which is isolated from the extract of citrus fruits peels. It exhibits a broad spectrum of biological activities such as anti-cancer, anti-inflammatory, cardiovascular protective and neuroprotective effects, however, its effect in melanogenesis remains uninvestigated. Melanin synthesis is a very important biological process in curing disease such ...
bioluminescence, etc ; chemical species; cysteine; fluorescence; luciferin; Show all 5 Subjects
Abstract:
... We report herein a caged luciferin probe Cy-Hy as a sequentially activated probe to selectively and sensitively sense l-Cys and H₂O₂. The probe displayed fluorescence and bioluminescence responses toward the two analytes. Utilizing the present probe, cellular excess l-Cys-induced H₂O₂ up-regulation was observed for the first time in living MDA-MB-231 cells. ...
bioluminescence, etc ; biosensors; detection limit; explosives; soil; Show all 5 Subjects
Abstract:
... We describe a miniaturized field-deployable biosensor module, designed to function as an element in a sensor network for standoff monitoring and mapping of environmental hazards. The module harbors live bacterial sensor cells, genetically engineered to emit a bioluminescent signal in the presence of preselected target materials, which act as its core sensing elements. The module, which detects and ...