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biosensors, etc ; DNA; Staphylococcus aureus; electrodes; gold; Show all 5 Subjects
Abstract:
... As one of the major foodborne pathogens, Staphylococcus aureus (S. aureus) can cause infectious diseases. In the current study, a novel electrochemical biosensor based on saltatory rolling circle amplification (SRCA) combined with CRISPR/Cas12a system was developed for the accurate detection of S. aureus. The thio-modified reporter probes (SH-ssDNA-MB) was immobilized on the surface of gold nanopa ...
biosensors, etc ; employment; forensic sciences; laboratory equipment; nanomaterials; Show all 5 Subjects
Abstract:
... Biosensors are compact analytical devices capable of transducing a biological interaction event into a measurable signal outcome in real-time. They can provide sensitive and affordable analysis of samples without the need for additional laboratory equipment or complex preparation steps. Biosensors may be beneficial for forensic analysis as they can facilitate large-scale high-throughput, sensitive ...
biosensors, etc ; color; colorimetry; point-of-care systems; Show all 4 Subjects
Abstract:
... Facile, efficient, and inexpensive biosensing systems are in high demand for biomedical test. In recent years, numerous smartphone-based biosensing systems have been developed to match demand for biomedical test in source-limited environment. However, application of these smartphone-based biosensing systems was limited because of performance gap between the smartphone-based systems and commercial ...
biosensors, etc ; disease diagnosis; electrochemiluminescence; food contamination; nanomaterials; Show all 5 Subjects
Abstract:
... The Electrochemiluminescence (ECL)-based biosensors have received considerable attention in food contaminants and disease diagnosis, due to their fascinating advantages such as low cost, fast analysis speed, wide linear range, high sensitivity, and excellent anti-interference ability. Meanwhile, with the vigorous development and improvement of nanotechnology, biosensor assembly strategies tend to ...
Kaory Barrientos; Juan Pablo Arango; Manuel Santiago Moncada; Jersson Placido; Juliana Patiño; Sara Legny Macías; Camilo Maldonado; Sandra Torijano; Sandra Bustamante; Martha Elena Londoño; Marisol Jaramillo
biosensors, etc ; biomarkers; detection limit; fluorescence; graphene; surface area; toxicity; Show all 7 Subjects
Abstract:
... Due to their fascinating chemical, optical, electrical, and biological properties carbon dots (CDs or CDots), carbon quantum dots (CQDs), and graphene quantum dots (GQDs) have attracted attention in biosensing as they can greatly improve the detection limit, sensitivity, and selectivity of biosensors. In general, CDs, CQDs, and GQDs are a class of carbon-based nanomaterials that are characterized ...
biosensors, etc ; detection limit; electrochemiluminescence; nanocomposites; nanoparticles; potassium persulfate; spermine; Show all 7 Subjects
Abstract:
... A ratiometric electrochemiluminescence strategy using a single luminophore for accurate and sensitive biomolecule detection could be immensely valuable in bioanalysis. Herein, an ultrasensitive ratiometric electrochemiluminescence sensing system was fabricated using a self-enhanced luminophore with dual-signal emission for the detection of spermine. A nanocomposite was synthesized by the covalent ...
biosensors, etc ; DNA; biomarkers; disease surveillance; enzyme activity; fluorescence; hydrolysis; microRNA; nanosilver; Show all 9 Subjects
Abstract:
... The development of a biosensing platform with high sensitivity, high specificity, and low cost for the detection of biomarkers, especially one that is programmable and universal, is critical for disease surveillance and diagnosis, yet it remains a difficulty. Herein, we combined the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system with a fluorescent label-free biosenso ...
biosensors, etc ; DNA; biocompatibility; blood serum; detection limit; erythrocytes; methylene blue; neoplasms; rapid methods; Show all 9 Subjects
Abstract:
... Circulating tumor cells (CTCs), as a type of tumor, have attracted wide attention because of their characteristics of shedding from the primary tumor and spreading to other tissues and organs through peripheral blood. The circulating tumor DNA (ctDNA), the DNA released by CTCs and other tumor cells into the peripheral blood, was considered as a promising detection substance for clinical applicatio ...
biosensors, etc ; Toxoplasma gondii; electrochemistry; parasites; point-of-care systems; rapid methods; toxoplasmosis; vertebrates; Show all 8 Subjects
Abstract:
... Toxoplasma gondii infection is a usual worldwide issue since a broad range of vertebrate hosts are infected by this famous parasite. However fetuses and immuno-compromised patients infected by parasite is of specific concern. Developing the easy-to-use, accurate, real time and selective methods for detection of toxoplasma infection has a key role in the treatment and management of patients. In thi ...
biosensors, etc ; Salmonella; chemical reactions; confidence interval; food chemistry; magnetism; microbial detection; orange juice; plate count; Show all 9 Subjects
Abstract:
... Phages are uniquely suited for bacterial detection due to their low cost and ability to recognize live bacteria. Herein, our work establishes the proof-of-concept detection of Salmonella in orange juice based on a phage-mediated portable magnetic relaxation switching (MRS) biosensor. The limit of quantification (LOQ) could reach 5 CFU/mL (95 % confidence interval [CI]: 4–7, N = 4) with a linear ra ...
biosensors, etc ; DNA; Shigella flexneri; detection limit; electrochemistry; electrodes; foodborne bacterial pathogens; indium tin oxide; melamine; sodium; Show all 10 Subjects
Abstract:
... An effective tool for early-stage selective detection of the foodborne bacterial pathogen Shigella flexneri (S. flexneri) is essential for diagnosing infectious diseases and controlling outbreaks. Here, a label-free electrochemical DNA biosensor for monitoring S. flexneri is developed. To fabricate the biosensor, detection probe (capture probe) is immobilized on the surface of poly melamine (P-Mel ...
... In this work, an electrochemiluminescence (ECL) biosensor with dual signal enhancement was constructed and used for DNA adenine methylation methyltransferase (Dam MTase) detection. At present of Dam MTase, restriction endonuclease (DPnI) cleaves hairpin DNA (HP) and releases the HP stem end as a single strand that can activate CRISPR/Cas12a trans-cleavage activity. Assisted by trans-cleavage, the ...
biosensors, etc ; anodes; biochemical oxygen demand; biofilm; carbon; carbon fibers; electron transfer; environment; microbial fuel cells; wastewater; Show all 10 Subjects
Abstract:
... Microbial fuel cell (MFC) has been extensively studied as a biosensor for determining biochemical oxygen demand (BOD). The method for quantifying BOD by employing coulombic yield (Q) of a bio-electrochemical degradation process obtained from MFC biosensors is referred to as BODQ. The physical structures of anode materials greatly affect the sensitivity and accuracy of the biosensor. In this work, ...
biosensors, etc ; energy transfer; fluorescence; food safety; graphene oxide; milk; mobile telephones; pathogenicity; safety assessment; single-stranded DNA; Show all 10 Subjects
Abstract:
... As a major threat to food safety due to their pathogenicity, foodborne bacteria have received much attention. In this paper, we present a one-step and wash-free microfluidic biosensor platform by smartphone for simultaneous multiple foodborne bacteria target single-stranded DNA (ssDNA) detection. This technology is based on the fluorescence resonance energy transfer (FRET) between the graphene oxi ...
... Organophosphates (OPs) are an integral part of modern agriculture; however, due to overexploitation, OPs pesticides residues are leaching and accumulating in the soil, and groundwater contaminated terrestrial and aquatic food webs. Acute exposure to OPs could produce toxicity in insects, plants, animals, and humans. OPs are known for covalent inhibition of acetylcholinesterase enzyme in pests and ...
biosensors, etc ; DNA; DNA probes; biomarkers; glassy carbon electrode; hybridization; lung neoplasms; microRNA; signal-to-noise ratio; Show all 9 Subjects
Abstract:
... In this study, the dual signal-labeled hairpin-structured DNA (dhDNA)-based probes have been developed to construct a novel nano-biosensor. This one hairpin-structured probe consists of a thiolated methylene blue-labeled hairpin capture probe (MB-HCP) as an inner reference probe and a ferrocene-modified anti-miRNA-21 DNA probe (Fc-AP-21). This novel integrated structure of MB-HCP and Fc-AP-21 was ...
biosensors, etc ; autoimmune diseases; biomarkers; central nervous system; cost effectiveness; disease control; disease diagnosis; electrophoresis; human resources; patients; sclerosis; Show all 11 Subjects
Abstract:
... Multiple sclerosis is a recurrent and progressive inflammatory autoimmune disease causing demyelination in the central nervous system. Nowadays, the number of MS patients is increasing, but the diagnostic process and disease management are still quite difficult and costly and time consuming. The combination of methods used for clinical MS diagnosis mainly relies on MRI, that cannot be used as rout ...
... Toxicosis through food and feed has remained a point of concern to food sectors across the globe. Although strict regulations have been implemented in developed and developing countries, their detection in food matrices is an evolving science. This study focuses on the development and fabrication of an electrochemical microfluidic biosensor that deploys aptamers to detect trace concentrations of A ...
biosensors, etc ; detection limit; electrochemistry; electrodes; fuel cells; glass; hydrogen peroxide; indium; nanoparticles; polymerization; silkworms; styrene; sulfonic acids; tap water; tin; water solubility; Show all 16 Subjects
Abstract:
... Herein, we demonstrate a high-accuracy H₂O₂ selective organic-inorganic 3D-heterointerface based on catalytically in-situ reduced Prussian-blue nanoparticles (PBNPs), Poly (3,4-ethylene dioxythiophene):poly (styrene sulfonic acid) (PEDOT:PSS) and water-soluble Silkworm protein (SWp). PBNPs were immobilized on an indium tin oxide-coated glass (ITO) electrode through the electrochemical polymerizati ...
biosensors, etc ; blood glucose; blood serum; carbon; chemical bonding; detection limit; dielectric spectroscopy; electrochemistry; electron microscopy; glucose; glucose oxidase; gold; health services; nanomaterials; voltammetry; Show all 15 Subjects
Abstract:
... With the development of blood glucose monitoring medical devices, higher requirements are put forward for the sensitivity and detection limit of biosensors. Herein, a high-performance flexible enzymatic glucose biosensor was prepared by electrochemical deposition of dendritic gold nanostructure on carbon cloth substrate, then covalently immobilizing glucose oxidase on the dendritic gold, and then ...