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1. Recent advances in applying cell-free systems for high-value and complex natural product biosynthesis

Author:
Xiangyang Ji; Wan-Qiu Liu; Jian Li
Source:
Current opinion in microbiology 2022 v.67 pp. 102142
ISSN:
1369-5274
Subject:
biosynthesis,   etc   ; enzymes; microbiology; toxicity;     Show all 4 Subjects
Abstract:
... Natural products have complex chemical structures and exhibit diverse bioactivities. To harness these high-value natural compounds, robust approaches are required for their rapid and sustainable production. Recently, cell-free systems as one promising solution have been applied to produce natural products by reconstitution of their entire biosynthetic pathways in vitro. Here, we highlight recent a ...
DOI:
10.1016/j.mib.2022.102142

2. Substrate promiscuity of acyltransferases contributes to the diversity of hydroxycinnamic acid derivatives in purple coneflower

Author:
Rao Fu; Pingyu Zhang; Ge Jin; Shuo Wei; Jiang Chen; Jin Pei; Yang Zhang
Source:
plant journal 2022 v.110 no.3 pp. 802-813
ISSN:
0960-7412
Subject:
biosynthesis,   etc   ; acyltransferases; coumaric acids;     Show all 3 Subjects
Abstract:
... High pliability and promiscuity are observed widely exist in plant specialized metabolism, especially the hydroxycinnamic acid metabolism. Here, we identified an addition BAHD acyltransferase (EpHMT) that catalyzes phaselic acid biosynthesis and found that the substrate promiscuities of identified BAHD and SCPL acyltransferases are responsible for the diversity of hydroxycinnamic acid derivatives ...
DOI:
10.1111/tpj.15704

3. A non-natural biosynthesis pathway toward 2-methylisoborneol

Author:
Binbin Gu; Jeroen S. Dickschat
Source:
Chemical communications 2022 v.58 no.27 pp. 4316-4319
ISSN:
1364-548X
Subject:
biosynthesis,   etc   ; 2-methylisoborneol; stereochemistry; stereoselectivity;     Show all 4 Subjects
Abstract:
... The biosynthesis of 2-methylisoborneol was reconstituted by elongation of dimethylallyl diphosphate (DMAPP) with (S)- and (R)-2-methylisopentenyl diphosphate (2-Me-IPP) using farnesyl diphosphate synthase (FPPS), followed by terpene cyclisation. The stereochemical course of the FPPS reaction was studied in detail using stereoselectively deuterated 2-Me-IPP isotopomers. ...
DOI:
10.1039/d2cc00636g

4. Biosynthesis of ethylene

Author:
Howard Thomas
Source:
Archives of biochemistry and biophysics 2022 pp. 109154
ISSN:
0003-9861
Subject:
biosynthesis,   etc   ; biophysics; crop production; ethylene;     Show all 4 Subjects
Abstract:
... The significance of the paper by Yu et al. (1979) is discussed in the context of the long history of ethylene as a plant growth regulator. By launching the era of molecular analysis and biotechnological exploitation, this research made a vital contribution to crop production and quality. ...
DOI:
10.1016/j.abb.2022.109154

5. CRISPR-based metabolic engineering in non-model microorganisms

Author:
Liangyu Lu; Xiaolin Shen; Xinxiao Sun; Yajun Yan; Jia Wang; Qipeng Yuan
Source:
Current opinion in biotechnology 2022 v.75 pp. 102698
ISSN:
0958-1669
Subject:
biosynthesis,   etc   ; biochemical pathways; biotechnology; genome;     Show all 4 Subjects
Abstract:
... Non-model microorganisms possess unique and versatile metabolic characteristics, offering great opportunities as cell factories for biosynthesis of target products. However, lack of efficient genetic tools for pathway engineering represents a big challenge to unlock the full production potential of these microbes. Over the past years, CRISPR systems have been extensively developed and applied to d ...
DOI:
10.1016/j.copbio.2022.102698

6. Out for a RiPP: challenges and advances in genome mining of ribosomal peptides from fungi

Author:
Simon C. Kessler; Yit-Heng Chooi
Source:
Natural product reports 2022 v.39 no.2 pp. 222-230
ISSN:
1460-4752
Subject:
biosynthesis,   etc   ; fungi; multigene family; peptides;     Show all 4 Subjects
Abstract:
... Covering up to June 2021 Ribosomally synthesized and post-translationally modified peptides (RiPPs) from fungi are an underexplored class of natural products, despite their propensity for diverse bioactivities and unique structural features. Surveys of fungal genomes for biosynthetic gene clusters encoding RiPPs have been limited in their scope due to our incomplete understanding of fungal RiPP bi ...
DOI:
10.1039/d1np00048a

7. Synthesis of rearranged indole diterpenes of the paxilline type

Author:
Devon J. Schatz; Eric J. Kuenstner; David T. George; Sergey V. Pronin
Source:
Natural product reports 2022 v.39 no.5 pp. 946-968
ISSN:
1460-4752
Subject:
biosynthesis,   etc   ; diterpenoids; fungi; metabolites; paxilline;     Show all 5 Subjects
Abstract:
... Rearranged indole diterpenes of the paxilline type comprise a large group of fungal metabolites that possess diverse structural features and potentially useful biological effects. The unique indoloterpenoid motif, which is common to all congeners, was first confirmed by crystallographic studies of paxilline. This family of natural products has fascinated organic chemists for the past four decades ...
DOI:
10.1039/d1np00062d

8. Two Steps to Bicyclo[4.2.0]octadienes from Cyclooctatetraene: Total Synthesis of Kingianic Acid A

Author:
Harshal D. Patel; Thomas Fallon
Source:
Organic letters 2022 v.24 no.12 pp. 2276-2281
ISSN:
1523-7052
Subject:
biosynthesis,   etc   ; byproducts; density functional theory;     Show all 3 Subjects
Abstract:
... Synthetic approaches to bicyclo[4.2.0]octadiene natural products frequently employ the synthesis of linear tetraenes to initiate a biosynthetic 8π/6π-electrocyclization cascade. This work forges a functionalized bicyclo[4.2.0]octadiene in two steps from cyclooctatetraene. The versatility of this method is demonstrated through natural product synthesis, including the first total synthesis of kingia ...
DOI:
10.1021/acs.orglett.2c00325

9. A highly selective 2′′-O-glycosyltransferase from Ziziphus jujuba and De novo biosynthesis of isovitexin 2′′-O-glucoside

Author:
Ya-Qun Zhang; Zi-Long Wang; Zhuo Chen; Zheng-Tong Jin; Aobulikasimu Hasan; Hai-Dong Wang; Yu-Wei Sun; Xue Qiao; Yong Wang; Min Ye
Source:
Chemical communications 2022 v.58 no.15 pp. 2472-2475
ISSN:
1364-548X
Subject:
biosynthesis,   etc   ; Escherichia coli; Ziziphus jujuba; glycosides;     Show all 4 Subjects
Abstract:
... A novel and efficient 2′′-O-glycosyltransferase ZjOGT38 was identified from Ziziphus jujuba. It could regio-selectively glycosylate 2-hydroxyflavanone C-glycosides. ZjOGT38 allowed de novo biosynthesis of isovitexin 2′′-O-glucoside in E. coli. ...
DOI:
10.1039/d1cc06949g

10. Cryptic Metabolites from Marine-Derived Microorganisms Using OSMAC and Epigenetic Approaches

Author:
Cristina Pinedo-Rivilla; Josefina Aleu; Rosa Durán-Patrón
Source:
Marine drugs 2022 v.20 no.2 pp. -
ISSN:
1660-3397
Subject:
biosynthesis,   etc   ; epigenetics; heterologous gene expression; metabolites;     Show all 4 Subjects
Abstract:
... Marine microorganisms have proven to be a source of new natural products with a wide spectrum of biological activities relevant in different industrial sectors. The ever-increasing number of sequenced microbial genomes has highlighted a discrepancy between the number of gene clusters potentially encoding the production of natural products and the actual number of chemically characterized metabolit ...
DOI:
10.3390/md20020084

11. Freedom of expression: A synthetic route to metabolites

Author:
Hannah E. Augustijn; Marnix H. Medema
Source:
Cell 2022 v.185 no.9 pp. 1449-1451
ISSN:
0092-8674
Subject:
biosynthesis,   etc   ; heterologous gene expression; metabolites; microbiome;     Show all 4 Subjects
Abstract:
... Microbial specialized metabolites play key roles in microbiome interactions, but their biosynthetic pathways are difficult to characterize. In this issue, Patel et al. (2022) describe new technologies for the computer-aided redesign of gene clusters to facilitate heterologous expression across diverse hosts and showcase their utility by identifying a new class of microbiome-derived nucleotide meta ...
DOI:
10.1016/j.cell.2022.04.007

12. SlWRKY35 positively regulates carotenoid biosynthesis by activating the MEP pathway in tomato fruit

Author:
Yong Yuan; Siyan Ren; Xiaofeng Liu; Liyang Su; Yu Wu; Wen Zhang; Yan Li; Yidan Jiang; Hsihua Wang; Rao Fu; Mondher Bouzayen; Mingchun Liu; Yang Zhang
Source:
The new phytologist 2022 v.234 no.1 pp. 164-178
ISSN:
0028-646X
Subject:
biosynthesis,   etc   ; fruits; genes; lutein; phytonutrients; tomatoes;     Show all 6 Subjects
Abstract:
... Carotenoids are vital phytonutrients widely recognised for their health benefits. Therefore, it is vital to thoroughly investigate the metabolic regulatory network underlying carotenoid biosynthesis and accumulation to open new leads towards improving their contents in vegetables and crops. The outcome of our study defines SlWRKY35 as a positive regulator of carotenoid biosynthesis in tomato. SlWR ...
DOI:
10.1111/nph.17977

13. A Fungal Promiscuous UbiA Prenyltransferase Expands the Structural Diversity of Chrodrimanin-Type Meroterpenoids

Author:
Kaijin Zhang; Guojian Zhang; Xuewen Hou; Chuanteng Ma; Junyu Liu; Qian Che; Tianjiao Zhu; Dehai Li
Source:
Organic letters 2022 v.24 no.10 pp. 2025-2029
ISSN:
1523-7052
Subject:
biosynthesis,   etc   ; dimethylallyltranstransferase; fungi; heterologous gene expression; meroterpenoids;     Show all 5 Subjects
Abstract:
... Prenyltransferases play important roles in the diversification of natural products and the improvement of biological activities. A UbiA-type prenyltransferase CdnC with substrate promiscuity was identified as the pivotal builder of the noncanonical chrodrimanin skeletons, which carry a benzo-cyclohexanone structure as the nonterpene part. In vitro and heterologous expression studies with CdnC led ...
DOI:
10.1021/acs.orglett.2c00495

14. Developmental Stages Affect the Capacity to Produce Aldehyde Green Leaf Volatiles in Zea mays and Vigna radiata

Author:
Jurgen Engelberth; Marie Engelberth
Source:
Plants 2022 v.11 no.4 pp. -
ISSN:
2223-7747
Subject:
biosynthesis,   etc   ; Vigna radiata; Zea mays; aldehydes; leaves;     Show all 5 Subjects
Abstract:
... Green leaf volatiles (GLV) are essentially produced by the green parts of plants upon damage. GLV are mainly 6-carbon molecules derived from fatty acids through the hydroperoxide lyase pathway and can serve as airborne signals to other parts of the same plant and to neighboring plants and help to protect them against biotic and abiotic stresses. However, while the biosynthesis is generally well un ...
DOI:
10.3390/plants11040526

15. Green approaches in synthesising nanomaterials for environmental nanobioremediation: Technological advancements, applications, benefits and challenges

Author:
Shams Forruque Ahmed; M. Mofijur; Nazifa Rafa; Anika Tasnim Chowdhury; Sidratun Chowdhury; Muntasha Nahrin; A.B.M. Saiful Islam; Hwai Chyuan Ong
Source:
Environmental research 2022 v.204 pp. 111967
ISSN:
0013-9351
Subject:
biosynthesis,   etc   ; biocompatibility; biomedical research; cytotoxicity; remediation; solvents;     Show all 6 Subjects
Abstract:
... Green synthesis approaches of nanomaterials (NMs) have received considerable attention in recent years as it addresses the sustainability issues posed by conventional synthesis methods. However, recent works of literature do not present the complete picture of biogenic NMs. This paper addresses the previous gaps by providing insights into the stability and toxicity of NMs, critically reviewing the ...
DOI:
10.1016/j.envres.2021.111967

16. Norditerpenoid alkaloids from Aconitum and Delphinium: structural relevance in medicine, toxicology, and metabolism

Author:
Ashraf M. A. Qasem; Ziyu Zeng; Michael G. Rowan; Ian S. Blagbrough
Source:
Natural product reports 2022 v.39 no.3 pp. 460-473
ISSN:
1460-4752
Subject:
biosynthesis,   etc   ; Aconitum; Delphinium; biotransformation; medicine; toxicity; toxicology;     Show all 7 Subjects
Abstract:
... Norditerpenoid alkaloids (NDA), typically N-ethylpiperidine containing C19 or C18 natural product diterpenes, are hexacycles with several contiguous often oxygenated stereocentres. As a function of their structural complexity, they display important pharmacological activities. The processed plants are used as important folk drugs and four NDAs have now been clinically approved. Many metabolism stu ...
DOI:
10.1039/d1np00029b

17. Preparation of quinazolinones using biosynthesized silver nanoparticles

Author:
Javad Safaei-Ghomi; Yasir Waleed Abdulhameed; Zianos Alisavari; Baram Ahmed Hamah Ameen; Seyyed Mohammad Ebrahimi
Source:
RSC advances 2022 v.12 no.20 pp. 12471-12476
ISSN:
2046-2069
Subject:
biosynthesis,   etc   ; Echium amoenum; ethanol; nanocatalysts; nanosilver; silver;     Show all 6 Subjects
Abstract:
... A silver nanocatalyst has been used as an effective catalyst for the preparation of quinazolinones under reflux conditions in ethanol. The catalyst was characterized by UV-VIS, FT-IR, XRD, SEM and EDS. Amongst the many benefits of this method are atom economy, reusability of the catalyst, low catalyst loading, applicability to a wide range of substrates, high yields of products, environmental frie ...
DOI:
10.1039/d2ra01629j

18. Role of gibberellin and its three GID1 receptors in Jasminum sambac stem elongation and flowering

Author:
Hongliang Zhang; Wei Wang; Jinfeng Huang; Yuting Wang; Li Hu; Yuan Yuan; Meiling Lyu; Binghua Wu
Source:
Planta 2022 v.255 no.1 pp. 17
ISSN:
0032-0935
Subject:
biosynthesis,   etc   ; Arabidopsis; Jasminum sambac; stem elongation; zeatin;     Show all 5 Subjects
Abstract:
... MAIN CONCLUSION: Taken together, our results establish a reciprocal relationship between vine elongation and flowering, and reveal that GA is a positive signal for stem elogation but a negative regulator of flowering in this species. Vines or climbing plants exhibit vigorous vegetative shoot extension. GA have long been recognized as an important signal for seasonal stem elongation and flowering i ...
DOI:
10.1007/s00425-021-03805-y

19. Alg mannosyltransferases: From functional and structural analyses to the lipid-linked oligosaccharide pathway reconstitution

Author:
Ning Wang; Sheng-Tao Li; Meng-Hai Xiang; Xiao-Dong Gao
Source:
Biochimica et biophysica acta 2022 v.1866 no.5 pp. 130112
ISSN:
0304-4165
Subject:
biosynthesis,   etc   ; endoplasmic reticulum; enzymology; glycosylation; mannosyltransferases; oligosaccharides; topology;     Show all 7 Subjects
Abstract:
... N-glycosylation is initiated from the biosynthesis of lipid-linked oligosaccharide (LLO) on the endoplasmic reticulum (ER), which is catalyzed by a series of Alg (asparagine-linked glycosylation) proteins. This review summarizes our recent studies on the enzymology of Alg mannosyltransferases (MTases). We also discuss the membrane topology and physiological importance of several ER cytosolic Alg p ...
DOI:
10.1016/j.bbagen.2022.130112

20. Aminoacylation of Indole Diterpenes by Cluster-Specific Monomodular NRPS-like Enzymes

Author:
Rose M. McLellan; Rosannah C. Cameron; Matthew J. Nicholson; Emily J. Parker
Source:
Organic letters 2022 v.24 no.12 pp. 2332-2337
ISSN:
1523-7052
Subject:
biosynthesis,   etc   ; aminoacylation; bioactive properties; diterpenoids; indoles; nonribosomal peptides;     Show all 6 Subjects
Abstract:
... Decoration of the core scaffolds of indole diterpene (IDT) natural products is key to generating structural and bioactivity diversity. Aminoacylation as a tailoring step is rarely linked to terpene biosynthesis and is extremely rare in IDT biosynthesis. Through heterologous pathway reconstruction, we have illuminated the genetic and biochemical basis for the only reported examples of aminoacylatio ...
DOI:
10.1021/acs.orglett.2c00473