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1. An ultra-sensitive test strip combining with RPA and CRISPR/Cas12a system for the rapid detection of GM crops

Author:
Jinbin Wang; Yu Wang; Hua Liu; Xiuwen Hu; Minghao Zhang; Xiaofeng Liu; Hailong Ye; Haijuan Zeng
Source:
Food control 2023 v.144 pp. 109383
ISSN:
0956-7135
Subject:
biotechnology,   etc   ; detection limit; food safety; genetically modified organisms; immunoassays; markets; plasmids; rapid methods;     Show all 8 Subjects
Abstract:
... Advanced molecular biotechnology has been developed to provide favorable technical support for genetically modified (GM) crop detection. For sensitive on-site detection, a Cas12a-based immunoassay strip was self-prepared and evaluated in this study. The strip was applied to analyze transgenic screening elements P-CaMV 35S and T-NOS by combination with RPA and Cas12a cleavage reaction. The assay ca ...
DOI:
10.1016/j.foodcont.2022.109383

2. Organophosphate pesticides an emerging environmental contaminant: Pollution, toxicity, bioremediation progress, and remaining challenges

Author:
Himanshu Mali; Chandni Shah; B.H. Raghunandan; Anil S. Prajapati; Darshan H. Patel; Ujjval Trivedi; R.B. Subramanian
Source:
Journal of environmental sciences (China) 2023 v.127 pp. 234-250
ISSN:
1001-0742
Subject:
biotechnology,   etc   ; acute exposure; bioremediation; biosensors; decontamination; environmental health; groundwater; humans; permeability; plant growth; pollution; soil; toxicity; China;     Show all 14 Subjects
Abstract:
... Organophosphates (OPs) are an integral part of modern agriculture; however, due to overexploitation, OPs pesticides residues are leaching and accumulating in the soil, and groundwater contaminated terrestrial and aquatic food webs. Acute exposure to OPs could produce toxicity in insects, plants, animals, and humans. OPs are known for covalent inhibition of acetylcholinesterase enzyme in pests and ...
DOI:
10.1016/j.jes.2022.04.023

3. Partial S(0)-driven autotrophic denitrification process facilitated the quick natural enrichment of anammox bacteria at room temperature

Author:
Zhiqi Wang; Jingfeng Gao; Huihui Dai; Yukun Yuan; Yifan Zhao; Dingchang Li; Yingchao Cui
Source:
Science of the total environment 2023 v.855 pp. 158916
ISSN:
0048-9697
Subject:
biotechnology,   etc   ; Thiobacillus; ambient temperature; ammonium; anaerobic ammonium oxidation; biofilm; denitrification; nitrates; nitrites; stable isotopes; sulfates; total nitrogen;     Show all 12 Subjects
Abstract:
... Anaerobic ammonium oxidation (anammox) is well-known to be an environmental and promising biotechnology. However, the natural enrichment of anammox bacteria is still a challenging topic. In this study, partial S(0)-driven autotrophic denitrification (PSAD) was developed to stably supply nitrite, and natural enrichment of anammox bacteria was rapidly realized in a single sequencing moving bed biofi ...
DOI:
10.1016/j.scitotenv.2022.158916

4. Anadenanthera colubrina (Vell) Brenan: Ethnobotanical, phytochemical, pharmacological and toxicological aspects

Author:
Merline Delices; Jessica de Araujo Isaias Muller; Karuppusamy Arunachalam; Domingos Tabajara de Oliveira Martins
Source:
Journal of ethnopharmacology 2023 v.300 no. pp. 115745
ISSN:
0378-8741
Subject:
National Center for Biotechnology Information,   etc   ; Anadenanthera colubrina; antioxidants; antiviral properties; bark; databases; environmental impact; ethnobotany; flavonoids; herbal medicines; indigenous species; insecticides; mechanism of action; therapeutics; toxicity; toxicology; traditional medicine; trees; triterpenoids; South America;     Show all 20 Subjects
Abstract:
... Anadenanthera colubrina (Vell.) Brenan is an endemic tree to South America and different parts of it are used by the population for the treatment of various diseases, as well as in indigenous rituals. This species has high pharmacological potential but may present toxic potential due to the presence of psychotropic compounds. To review published studies with the species A. colubrina regarding ethn ...
DOI:
10.1016/j.jep.2022.115745

5. Biosensor-enabled pathway optimization in metabolic engineering

Author:
Yuxi Teng; Jianli Zhang; Tian Jiang; Yusong Zou; Xinyu Gong; Yajun Yan
Source:
Current opinion in biotechnology 2022 v.75 pp. 102696
ISSN:
0958-1669
Subject:
biotechnology,   etc   ; biosensors; metabolites;     Show all 3 Subjects
Abstract:
... Microbes can convert inexpensive renewable substrates to valuable metabolites by their natural metabolic pathways. To maximize the productivity, the pathways yet require further optimization, which remains challenging for our limited knowledge of complex biology. Genetically encoded biosensors are able to detect metabolite concentrations or environmental changes and transfer these inputs to measur ...
DOI:
10.1016/j.copbio.2022.102696

6. Perspectives for better batch effect correction in mass-spectrometry-based proteomics

Author:
Ser-Xian Phua; Kai-Peng Lim; Wilson Wen-Bin Goh
Source:
Computational and Structural Biotechnology Journal 2022 v.20 pp. 4369-4375
ISSN:
2001-0370
Subject:
biotechnology,   etc   ; algorithms; proteomics;     Show all 3 Subjects
Abstract:
... Mass-spectrometry-based proteomics presents some unique challenges for batch effect correction. Batch effects are technical sources of variation, can confound analysis and usually non-biological in nature. As proteomic analysis involves several stages of data transformation from spectra to protein, the decision on when and what to apply batch correction on is often unclear. Here, we explore severa ...
DOI:
10.1016/j.csbj.2022.08.022

7. Thermodynamic database supports deciphering protein-nucleic acid interactions

Author:
Long-Can Mei; Ge-Fei Hao; Guang-Fu Yang
Source:
Trends in biotechnology 2022 pp. -
ISSN:
0167-7799
Subject:
biotechnology,   etc   ; databases; thermodynamics;     Show all 3 Subjects
Abstract:
... The thermodynamics of protein–nucleic acid interactions are crucial for elucidating the mechanisms of molecular recognition and pathological consequences. The Protein-Nucleic Acid Thermodynamics Database (PNATDB) is a thermodynamics database containing experimentally determined thermodynamic parameters along with sequence, structural, and function data, which is available free online. ...
DOI:
10.1016/j.tibtech.2022.09.018

8. The cell density effect in animal cell-based bioprocessing: Questions, insights and perspectives

Author:
Jesús Lavado-García; Pol Pérez-Rubio; Laura Cervera; Francesc Gòdia
Source:
Biotechnology advances 2022 v.60 pp. 108017
ISSN:
0734-9750
Subject:
biotechnology,   etc   ; animals; bioprocessing;     Show all 3 Subjects
Abstract:
... One of the main challenges in the development of bioprocesses based on cell transient expression is the commonly reported reduction of cell specific productivity at increasing cell densities. This is generally known as the cell density effect (CDE). Many efforts have been devoted to understanding the cell metabolic implications to this phenomenon in an attempt to design operational strategies to o ...
DOI:
10.1016/j.biotechadv.2022.108017

9. Conifer Biotechnology: An Overview

Author:
Sonia María Rodríguez; Ricardo Javier Ordás; José Manuel Alvarez
Source:
Forests 2022 v.13 no.7 pp. -
ISSN:
1999-4907
Subject:
biotechnology,   etc   ; computers; conifers;     Show all 3 Subjects
Abstract:
... The peculiar characteristics of conifers determine the difficulty of their study and their great importance from various points of view. However, their study faces numerous important scientific, methodological, cultural, economic, social, and legal challenges. This paper presents an approach to several of those challenges and proposes a multidisciplinary scientific perspective that leads to a holi ...
DOI:
10.3390/f13071061

10. The LEGO® brick road to open science and biotechnology

Author:
Etienne Boulter; Julien Colombelli; Ricardo Henriques; Chloé C. Féral
Source:
Trends in biotechnology 2022 v.40 no.9 pp. 1073-1087
ISSN:
0167-7799
Subject:
biotechnology,   etc   ; bricks; ecosystems;     Show all 3 Subjects
Abstract:
... LEGO® is a brand of toys that have entertained generations of children. Beyond amusement, LEGO® bricks also constitute a building ecosystem of their own that creators from the general public, as well as scientists and engineers, can use to design and assemble devices for all purposes, including scientific research and biotechnology. We describe several of these constructions to highlight the const ...
DOI:
10.1016/j.tibtech.2022.02.003

11. Powering biological nitrogen removal from the environment by geobatteries

Author:
Shun Li; Andreas Kappler; Stefan B. Haderlein; Yong-Guan Zhu
Source:
Trends in biotechnology 2022 v.40 no.4 pp. 377-380
ISSN:
0167-7799
Subject:
biotechnology,   etc   ; nitrogen; wastewater;     Show all 3 Subjects
Abstract:
... Geobatteries are redox-active substances that can take up, store, and release electrons reversibly. Provided that their redox activity can be maintained by fluctuations of oxidizing and reducing redox conditions, geobatteries could also improve the performance of engineered systems, such as in biological nitrogen removal from wastewater or constructed wetlands. ...
DOI:
10.1016/j.tibtech.2021.10.008

12. Protein aggregation and crystallization with ionic liquids: Insights into the influence of solvent properties

Author:
Qi Han; Stuart J. Brown; Calum J. Drummond; Tamar L. Greaves
Source:
Journal of colloid and interface science 2022 v.608 pp. 1173-1190
ISSN:
0021-9797
Subject:
biotechnology,   etc   ; crystallization; medicine;     Show all 3 Subjects
Abstract:
... Ionic liquids (ILs) have been used in solvents for proteins in many applications, including biotechnology, pharmaceutics, and medicine due to their tunable physicochemical and biological properties. Protein aggregation is often undesirable, and predominantly occurs during bioprocesses, while the aggregation process can be reversible or irreversible and the aggregates formed can be native/non-nativ ...
DOI:
10.1016/j.jcis.2021.10.087

13. Richness estimation in microbiome data obtained from denoising pipelines

Author:
Sven Kleine Bardenhorst; Marius Vital; André Karch; Nicole Rübsamen
Source:
Computational and Structural Biotechnology Journal 2022 v.20 pp. 508-520
ISSN:
2001-0370
Subject:
biotechnology,   etc   ; bioinformatics; microbiome;     Show all 3 Subjects
Abstract:
... The quantification of richness within a sample—either measured as the number of observed species or approximated by estimation—is a common first step in microbiome studies and is known to be highly dependent on sequencing depth, which itself is highly variable between samples. Rarefaction curves serve as a tool to investigate this dependency and it is often argued that after rarefying data—sub-sam ...
DOI:
10.1016/j.csbj.2021.12.036

14. Dynamically regulating metabolic fluxes with synthetic metabolons

Author:
Youjun Zhang; Alisdair R. Fernie
Source:
Trends in biotechnology 2022 v.40 no.9 pp. 1019-1020
ISSN:
0167-7799
Subject:
biotechnology,   etc   ; RNA; enzymes;     Show all 3 Subjects
Abstract:
... Enzyme–enzyme assemblies commonly occur naturally, yet the factors that lead to their transient nature are not fully understood. Mitkas et al. have shown how clustered regularly interspaced short palindromic repeats (CRISPR) enzymes and RNA scaffolds allow synthetic enzyme complexes to be formed and disassembled as needed, providing powerful new tools for metabolic engineering. ...
DOI:
10.1016/j.tibtech.2022.06.005

15. (R)evolution-on-a-chip

Author:
Evgenios Bouzetos; Ketan Ashok Ganar; Enrico Mastrobattista; Siddharth Deshpande; John van der Oost
Source:
Trends in biotechnology 2022 v.40 no.1 pp. 60-76
ISSN:
0167-7799
Subject:
biotechnology,   etc   ; evolution; microfluidic technology;     Show all 3 Subjects
Abstract:
... Billions of years of Darwinian evolution has led to the emergence of highly sophisticated and diverse life forms on Earth. Inspired by natural evolution, similar principles have been adopted in laboratory evolution for the fast optimization of genes and proteins for specific applications. In this review, we highlight state-of-the-art laboratory evolution strategies for protein engineering, with a ...
DOI:
10.1016/j.tibtech.2021.04.009

16. Are integrated bioelectrochemical technologies feasible for wastewater management?

Author:
Ravi K. Yadav; Sovik Das; Sunil A. Patil
Source:
Trends in biotechnology 2022 pp. -
ISSN:
0167-7799
Subject:
biotechnology,   etc   ; energy; wastewater treatment;     Show all 3 Subjects
Abstract:
... The need for sustainable technological solutions for wastewater management at different scales has led to the emergence of several promising integrated bioelectrochemical technologies in the past decade. A thorough assessment of these technologies is imperative to understand their practical implementation feasibility and to identify the key challenges to prioritise the research and development wor ...
DOI:
10.1016/j.tibtech.2022.09.001

17. Attractor detection and enumeration algorithms for Boolean networks

Author:
Tomoya Mori; Tatsuya Akutsu
Source:
Computational and Structural Biotechnology Journal 2022 v.20 pp. 2512-2520
ISSN:
2001-0370
Subject:
biotechnology,   etc   ; genes; mathematical models;     Show all 3 Subjects
Abstract:
... The Boolean network (BN) is a mathematical model used to represent various biological processes such as gene regulatory networks. The state of a BN is determined from the previous state and eventually reaches a stable state called an attractor. Due to its significance for elucidating the whole system, extensive studies have been conducted on analysis of attractors. However, the problem of detectin ...
DOI:
10.1016/j.csbj.2022.05.027

18. Design of erythrocyte-derived carriers for bioimaging applications

Author:
Wing-Fu Lai; Dahong Zhang; Wing-Tak Wong
Source:
Trends in biotechnology 2022 pp. -
ISSN:
0167-7799
Subject:
biotechnology,   etc   ; bioimaging; drugs; erythrocytes;     Show all 4 Subjects
Abstract:
... Erythrocytes are physiological entities that have been exploited in both preclinical and clinical trials for the delivery of exogenous agents. Over the years, diverse erythrocyte-derived carriers (ECs) have been developed with related patents granted for industrial and commercial purposes. However, most ECs have only been exploited for drug delivery. Serious discussions regarding their application ...
DOI:
10.1016/j.tibtech.2022.07.010

19. Digital models in biotechnology: Towards multi-scale integration and implementation

Author:
Fabian S.F. Hartmann; Isuru A. Udugama; Gerd M. Seibold; Hirokazu Sugiyama; Krist V. Gernaey
Source:
Biotechnology advances 2022 v.60 pp. 108015
ISSN:
0734-9750
Subject:
biotechnology,   etc   ; fluid mechanics; industry;     Show all 3 Subjects
Abstract:
... Industrial biotechnology encompasses a large area of multi-scale and multi-disciplinary research activities. With the recent megatrend of digitalization sweeping across all industries, there is an increased focus in the biotechnology industry on developing, integrating and applying digital models to improve all aspects of industrial biotechnology. Given the rapid development of this field, we syst ...
DOI:
10.1016/j.biotechadv.2022.108015

20. Effects of Surface Tethering on the Thermodynamics and Kinetics of Frustrated Protein Folding

Author:
Jiang Du; Hongmei Yin; Yanfang Lu; Teng Lu; Tao Chen
Source:
Journal of physical chemistry 2022 v.126 no.26 pp. 4776-4786
ISSN:
1520-5207
Subject:
biotechnology,   etc   ; diffusivity; immunity; thermodynamics;     Show all 4 Subjects
Abstract:
... The interaction between the protein and surface plays an important role in biology and biotechnology. To understand how surface tethering influences the folding behavior of frustrated proteins, in this work, we systematically study the thermodynamics and folding kinetics of the bacterial immunity protein Im7 and Fyn SH3 domain tethered to a surface using Langevin dynamics simulations. Upon surface ...
DOI:
10.1021/acs.jpcb.2c01982