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1. In Situ Genetically Cascaded Amplification for Imaging RNA Subcellular Locations

Author:
Ren, Kewei; Wu, Rigumula; Karunanayake Mudiyanselage, Aruni P. K. K.; Yu, Qikun; Zhao, Bin; Xie, Yiwen; Bagheri, Yousef; Tian, Qian; You, Mingxu
Source:
Journal of the American Chemical Society 2020 v.142 no.6 pp. 2968-2974
ISSN:
1520-5126
Subject:
broccoli,   etc   ; RNA; cytotoxicity; fluorescence; hybridization chain reaction; image analysis; mammals;     Show all 7 Subjects
Abstract:
... In situ amplification methods, such as hybridization chain reaction, are valuable tools for mapping the spatial distribution and subcellular location of target analytes. However, the live-cell applications of these methods are still limited due to challenges in the probe delivery, degradation, and cytotoxicity. Herein, we report a novel genetically encoded in situ amplification method to noninvasi ...
DOI:
10.1021/jacs.9b11748

2. A method for obtaining flexible broccoli varieties for sustainable agriculture

Author:
Ciancaleoni, Simona; Negri, Valeria
Source:
BMC genetics 2020 v.21 no.1 pp. 51
ISSN:
1471-2156
Subject:
broccoli,   etc   ; Brassica oleracea; breeding programs; genetic variation; landraces; low input agriculture; microsatellite repeats; sustainable agriculture; Italy;     Show all 9 Subjects
Abstract:
... BACKGROUND: The use of high inputs in agriculture resulted in few varieties (hybrids and pure lines) used in all agricultural systems. Also varieties of vegetables, including broccoli, for organic and low-input agriculture, are almost exclusively hybrids, since there are very few specific breeding programs and varieties for sustainable agriculture systems. A strategy to overcome this issue is the ...
DOI:
10.1186/s12863-020-00846-2

3. Subtle sequence variations alter tripartite complex kinetics and G-quadruplex dynamics in RNA aptamer Broccoli

Author:
Savage, Jonathan C.; Davare, Monika A.; Shinde, Ujwal
Source:
Chemical communications 2020 v.56 no.17 pp. 2634-2637
ISSN:
1364-548X
Subject:
broccoli,   etc   ; RNA; chemical compounds; chemical reactions; fluorescence; germplasm conservation; oligonucleotides; spectroscopy; spinach;     Show all 9 Subjects
Abstract:
... Though extensively utilized, the fluorescent RNA aptamer Broccoli is poorly characterized with an unknown structure. Spectroscopic and kinetic investigations of tripartite complex formation reveal surprising differences between Broccoli and Spinach aptamers despite extreme sequence conservation. Our studies highlight how subtle sequence variations impart functional consequences of G-quadruplex–cat ...
DOI:
10.1039/c9cc09375c

4. Consider a broccoli stalk: How the concept of edibility influences quantification of household food waste

Author:
Moreno Laura, C.; Moreno, Laura C.; Tran, Thao; Potts, Matthew D.
Source:
Journal of environmental management 2020 v.256 pp. 109977
ISSN:
0301-4797
Subject:
broccoli,   etc   ; food waste; foods; issues and policy; waste management; Colorado; New York;     Show all 7 Subjects
Abstract:
... Food waste measurement and policy often seek to differentiate between edible food and associated inedible parts, acknowledging different underlying causes for discard and different preferred solutions for waste management. Given the varying views of edibility within and across cultures, there is no widely agreed upon or universal categorization. To understand how edibility influences the outcome o ...
DOI:
10.1016/j.jenvman.2019.109977

5. The effects of cooking method on selected quality traits of broccoli and green asparagus

Author:
Danowska‐Oziewicz, Marzena; Narwojsz, Agnieszka; Draszanowska, Anna; Marat, Natalia
Source:
International journal of food science & technology 2020 v.55 no.1 pp. 127-135
ISSN:
0950-5423
Subject:
broccoli,   etc   ; air; antioxidant activity; boiling; carotenoids; chlorophyll; color; cooking; microwave ovens; polyphenols; sensory properties; steam;     Show all 12 Subjects
Abstract:
... Broccoli and green asparagus were cooked using boiling water, steam, microwave oven and combi oven with overheated steam and combination of hot air with overheated steam (only broccoli). Vegetables were analysed for chlorophylls a and b, carotenoids, total polyphenols, antioxidant activity, colour parameters and sensory properties. Cooking in the combi oven with overheated steam was an effective m ...
DOI:
10.1111/ijfs.14269

6. Improving Spinach2-and Broccoli-based biosensors for single and double analytes

Author:
Yuan, Shuo-Fu; Alper, Hal S.
Source:
Biotechnology notes 2020 v.1 pp. 2-8
ISSN:
2665-9069
Subject:
broccoli,   etc   ; RNA; biosensors; humans; magnesium; metabolites; oligonucleotides; screening; streptavidin; thrombin; tyrosine;     Show all 11 Subjects
Abstract:
... The use of “Spinach”-based RNA sensors for the detection of small metabolites and proteins has received growing interest in the recent years. While this approach can be used in vivo for cell sensing and in vitro for microfluidic assays, their overall utility is limited as a result of a high magnesium ion dependence. Here, we alleviate this limitation through incorporating a human tRNAᴸʸˢ³ or an en ...
DOI:
10.1016/j.biotno.2020.01.001

7. Optimal design and experiment of 2-DoF five-bar mechanism for flower seedling transplanting

Author:
Zhao, Xiong; Guo, Junlin; Li, Kewei; Dai, Li; Chen, Jianneng
Source:
Computers and electronics in agriculture 2020 v.178 pp. 105746
ISSN:
0168-1699
Subject:
broccoli,   etc   ; Brassica oleracea; actuators; algorithms; automation; control methods; equipment; flowers; mathematical models; seedling transplanting; seedlings; working conditions;     Show all 12 Subjects
Abstract:
... The currently used large-scale flower seedling transplanting equipment is expensive and has poor adaptability, while the small-scale transplanting equipment has problems with seedling damage and low automation. In view of this situation, a 2-DoF five-bar transplanting mechanism for multiple-point picking to single-point planting was designed. In a motion cycle, the seedlings could be picked from t ...
DOI:
10.1016/j.compag.2020.105746

8. The delayed senescence of postharvest buds in salt ions was related to antioxidant activity, HDA9 and CCX1 in broccoli (Brassica oleracea L. var. Italic Planch.)

Author:
Yan, Kai; Ran, Maolin; Li, Shengnan; Zhang, Junli; Wang, Yu; Wang, Zhimin; Wei, Dayong; Tang, Qinglin
Source:
Food chemistry 2020 v.324 pp. 126887
ISSN:
0308-8146
Subject:
broccoli,   etc   ; Brassica oleracea; antioxidant activity; buds; calcium chloride; chlorophyll; enzyme inhibitors; epigenetics; histone deacetylase; malondialdehyde; peroxidase; potassium chloride; superoxide dismutase;     Show all 13 Subjects
Abstract:
... Epigenetic regulation and salt ions play essential roles in senescence control, but the underlying regulatory mechanism of senescence has not been thoroughly revealed in broccoli postharvest buds. Here, we found 200 mmol·L⁻¹ NaCl, 400 mmol·L⁻¹ KCl, 40 mmol·L⁻¹ CaCl₂ and 0.5 μmol·L⁻¹ Trichostatin-A (TSA, a histone deacetylase inhibitor) delayed the bud senescence. They resulted in significantly inh ...
DOI:
10.1016/j.foodchem.2020.126887

9. Climate and host plants mediating seasonal dynamics and within-plant distribution of the diamondback moth (Plutella xylostella)

Author:
Farias, Elizeu S.; Santos, Abraão A.; Ribeiro, Arthur V.; Carmo, Daiane G.; Paes, Jhersyka S.; Picanço, Marcelo C.
Source:
Crop protection 2020 v.134 pp. 105172
ISSN:
0261-2194
Subject:
broccoli,   etc   ; Plutella xylostella; cabbage; cauliflower; climatic factors; cole crops; cultural control; host plants; insecticides; pests; phenology; relative humidity; temperature; Brazil;     Show all 14 Subjects
Abstract:
... The diamondback moth (DBM), Plutella xylostella L. (Lepidoptera: Plutellidae), is the most destructive pest of brassicas worldwide. Cultural control of this pest is important due to the reported resistance of DBM strains to most insecticides and should be devised from basic information related to the pest. This study investigated the influence of the host plant and climate in the seasonal dynamics ...
DOI:
10.1016/j.cropro.2020.105172

10. Influence of soil tillage on natural regulation of the cabbage root fly Delia radicum in brassicaceous crops

Author:
Mesmin, Xavier; Cortesero, Anne-Marie; Daniel, Loïc; Plantegenest, Manuel; Faloya, Vincent; Le Ralec, Anne
Source:
Agriculture, ecosystems & environment 2020 v.293 pp. 106834
ISSN:
0167-8809
Subject:
broccoli,   etc   ; Araneae; Brassica oleracea; Carabidae; Delia radicum; arthropods; crops; insect pests; larvae; pest control; plant protection; predators; spring; tillage;     Show all 14 Subjects
Abstract:
... Ground dwelling predators provide regulation services of several insect pests. Enhancing these services may be a step toward integrated crop protection. Many studies have shown that soil tillage is deleterious to ground dwelling predators but pest regulation processes and services have rarely been measured. We performed an experiment to study whether simplifying soil tillage before the establishme ...
DOI:
10.1016/j.agee.2020.106834

11. Potential of jasmonic acid (JA) in accelerating postharvest yellowing of broccoli by promoting its chlorophyll degradation

Author:
Fang, Huixin; Luo, Feng; Li, Pengxia; Zhou, Qian; Zhou, Xin; Wei, Baodong; Cheng, Shunchang; Zhou, Hongsheng; Ji, Shujuan
Source:
Food chemistry 2020 v.309 pp. 125737
ISSN:
0308-8146
Subject:
broccoli,   etc   ; Brassica oleracea; allene-oxide cyclase; chlorophyll; flowers; fluorescence; gene expression regulation; genes; jasmonic acid; methyl jasmonate;     Show all 10 Subjects
Abstract:
... Chlorophyll degradation is the main reason for postharvest yellowing of broccoli. To uncover the role of jasmonic acid (JA) on the degradation of chlorophyll, broccoli flowers were treated with exogenous methyl jasmonate (MeJA) and diethyldithiocarbamic acid (DIECA). We found a surge of endogenous JA content with the yellowing process, and a significant correlation between JA and chlorophyll conte ...
DOI:
10.1016/j.foodchem.2019.125737

12. Profiling glucosinolate metabolites in human urine and plasma after broccoli consumption using non-targeted and targeted metabolomic analyses

Author:
Sun, Jianghao; Charron, Craig S.; Novotny, Janet A.; Peng, Bing; Yu, Liangli; Chen, Pei
Source:
Food chemistry 2020 v.309 pp. 125660
ISSN:
0308-8146
Subject:
broccoli,   etc   ; Brassica oleracea; cysteine; florets; glucoraphanin; hippuric acid; humans; indoles; mass spectrometry; metabolites; metabolomics; neoplasms; physiological response; sulforaphane; urine;     Show all 15 Subjects
Abstract:
... Broccoli is a popular brassica vegetable and its consumption may decrease the occurrence of cancer in certain populations. To gain insight into the metabolites that may induce physiological responses to broccoli intake, a non-targeted metabolomic approach and a targeted approach for analysis of glucosinolate metabolites were developed using high resolution accurate mass spectrometry. A human study ...
DOI:
10.1016/j.foodchem.2019.125660
PubMed:
31670121
PubMed Central:
PMC6921088

13. Antioxidant properties and sensory evaluation of microgreens from commercial and local farms

Author:
Tan, Libo; Nuffer, Holly; Feng, Jiannan; Kwan, Shu Hang; Chen, Hsiangting; Tong, Xiao; Kong, Lingyan
Source:
Food science and human wellness 2020 v.9 no.1 pp. 45-51
ISSN:
2213-4530
Subject:
broccoli,   etc   ; antioxidant activity; ascorbic acid; chlorophyll; color; farms; flavor; herbs; humans; hydroponics; microgreens; nutrition; nutritive value; purchasing; sensory evaluation; taste;     Show all 16 Subjects
Abstract:
... Microgreens are young and tender vegetables or herbs that provide attractive color, flavor, and nutrition. The purpose of this study was to evaluate the nutritional and sensory qualities of broccoli microgreens grown by different methods (hydroponically vs. soil grown) and from different sources (commercial vs. local farm). No significant difference in total phenolic concentration and antioxidant ...
DOI:
10.1016/j.fshw.2019.12.002

14. Emerging techniques applied to by-products for food fortification

Author:
Marinelli, Valeria; Spinelli, Sara; Angiolillo, Luisa; Del Nobile, Matteo Alessandro; Conte, Amalia
Source:
Journal of food science and technology 2020 v.57 no.3 pp. 905-914
ISSN:
0022-1155
Subject:
broccoli,   etc   ; antioxidant activity; byproducts; consumer demand; cooking; fish patties; flavonoids; food fortification; healthy diet; maltodextrins; microencapsulation; nutritive value; temperature;     Show all 13 Subjects
Abstract:
... Considering the increasing consumer demand for healthy food, the extract from broccoli by-products was studied. To this aim, in the first step, three extraction techniques were compared in terms of extraction efficiency. The best method was the pressurized liquid extraction. Then, the extract microencapsulation was optimized in terms of type of wall material (between Capsul and maltodextrins), con ...
DOI:
10.1007/s13197-019-04123-8
PubMed:
32123411
PubMed Central:
PMC7026334

15. Changes in growth kinetics and motility characteristics of Escherichia coli in the presence of sulphoraphane isolated from broccoli seed meal

Author:
García‐Saldaña, Jesús Santos; Parra‐Delgado, Julián; Campas‐Baypoli, Olga Nydia; Sánchez‐Machado, Dalia Isabel; Cantú‐Soto, Ernesto Uriel; López‐Cervantes, Jaime
Source:
International journal of food science & technology 2020 v.55 no.2 pp. 851-860
ISSN:
0950-5423
Subject:
broccoli,   etc   ; Brassica oleracea; Escherichia coli; antibacterial properties; bacteria; bacterial motility; growth models; meals (products); specific growth rate; sulforaphane;     Show all 10 Subjects
Abstract:
... In this investigation, the purified sulphoraphane from broccoli seed meal was evaluated in a range of 5–400 μg mL⁻¹ against Escherichia coli. The results showed that levels above 200 μg mL⁻¹ can inhibit 100% growth of 10⁶ CFU mL⁻¹ up to 5 h and cause a deformation of sigmoid behaviour in the growth kinetics, and even the bacterial population is reduced. On the other hand, low sulphoraphane levels ...
DOI:
10.1111/ijfs.14372

16. Dynamic variation of glucosinolates and isothiocyanates in broccoli sprouts during hydrolysis

Author:
Leng, Chaoqun; Zhang, Yuxuan; Wang, Mian; Wang, Pei; Gu, Zhenxin; Yang, Runqiang
Source:
Scientia horticulturae 2019 v.255 pp. 128-133
ISSN:
0304-4238
Subject:
broccoli,   etc   ; Brassica oleracea var. italica; gluconapin; glucoraphanin; hydrolysis; isothiocyanates; nitriles;     Show all 7 Subjects
Abstract:
... In this study, the glucosinolates (GLs) content and isothiocyanates (ITCs) formation in broccoli sprouts and their dynamic variation during hydrolysis were investigated. The results showed that 7 kinds of GLs were detected and glucoraphanin (GRA) was the dominating component. After hydrolysis, three kinds of ITCs and 2 kinds of nitriles were detected in broccoli sprouts, however, the corresponding ...
DOI:
10.1016/j.scienta.2019.05.026

17. Comparison of Parent and Child Ratings of Fruit and Vegetable Liking to Assess Parent Accuracy as Proxy Reporters

Author:
Stage, Virginia C.; Downing, Carrie; Hegde, Archana V.; Dev, Dipti A.; Peterson, Amanda D.; Goodell, L. Suzanne
Source:
Ecology of food and nutrition 2019 v.58 no.2 pp. 166-186
ISSN:
1543-5237
Subject:
broccoli,   etc   ; fruits; grapes; oranges; parents; preschool children; preschools; North Carolina;     Show all 8 Subjects
Abstract:
... This study examined the accuracy of parent-report child fruit/vegetable (FV) liking. Child/parent dyads (n = 24) were recruited from six Head Start preschools in North Carolina. Liking for 10 FVs was assessed using a validated pictorial tool for children; a similar scale was used for parents. Negative relationships were observed between parent/child for one fruit (grapes) and one vegetable (brocco ...
DOI:
10.1080/03670244.2019.1572002

18. Broccoli byproducts for protection and co-delivery of EGCG and tuna oil

Author:
Shi, Meng; Ying, Dan-Yang; Ye, Jian-Hui; Sanguansri, Luz; Augustin, Mary Ann
Source:
Food chemistry 2020 v.326 pp. 126963
ISSN:
0308-8146
Subject:
broccoli,   etc   ; bioavailability; byproducts; digesta; emulsions; epigallocatechin gallate; food waste; in vitro digestion; ingredients; intestines; lipid content; oxidation; oxidative stability; oxygen; powders; tuna oil; waste reduction;     Show all 17 Subjects
Abstract:
... Neat epigallocatechin gallate (EGCG) has low bioavailability and tuna oil (TO) is prone to oxidation. Broccoli byproducts (BBP) were used for preparing TO-BBP (25% oil, dry basis) and TO-EGCG-BBP (20% oil and 20% EGCG, dry basis) powders. The gross composition and surface fat of powders and morphology of reconstituted emulsions were characterized. Oxipres® data (80 °C, 5 bar oxygen pressure) showe ...
DOI:
10.1016/j.foodchem.2020.126963

19. Is there a best technique to cook vegetables? – A study about physical and sensory aspects to stimulate their consumption

Author:
Torres de Castro, Neide; de Lacerda, Lívia; Rodrigues de Alencar, Ernandes; Assunção Botelho, Raquel Braz
Source:
International journal of gastronomy and food science 2020 v.21 pp. 100218
ISSN:
1878-450X
Subject:
broccoli,   etc   ; Brassica oleracea; Cucurbita moschata; Daucus carota; boiling; carrots; color; gastronomy; microwave cooking; microwave treatment; nutrient content; nutrients; ovens; sensory evaluation; steaming; taste; texture; zucchini; Brazil;     Show all 19 Subjects
Abstract:
... Consumers are becoming more health-conscious and have less time to prepare meals. They are often confused about which time and cooking method are adequate to preserve nutrients in vegetable. All cooking techniques cause changes in the nutrient content and the taste of a vegetable. The objective of this study was to determine the best cooking method for different vegetables using sensory evaluation ...
DOI:
10.1016/j.ijgfs.2020.100218

20. Glucosinolates and phenolic compounds rich broccoli extract: Encapsulation by electrospraying and antitumor activity against glial tumor cells

Author:
Radünz, Marjana; Hackbart, Helen Cristina Dos Santos; Bona, Natália Pontes; Pedra, Nathalia Stark; Hoffmann, Jessica Fernanda; Stefanello, Francieli Moro; Da Rosa Zavareze, Elessandra
Source:
Colloids and surfaces 2020 v.192 pp. 111020
ISSN:
0927-7765
Subject:
broccoli,   etc   ; active sites; antineoplastic activity; astrocytes; cell culture; cytotoxicity; disease control; electrospraying; encapsulation; glioma; glucosinolates; metabolism; moieties; neoplasm cells; phenolic compounds; thermal stability; zein;     Show all 17 Subjects
Abstract:
... Gliomas, intracranial malignant tumors, are aggressive, asymptomatic and difficult to treat due to their degree of infiltration, alternatives are needed to treat the disease. In this sense, natural compounds from the specialized metabolism of plants can act to control the disease. Glucosinolates and phenolic compounds, present in broccoli, have a potential to promote tumor cell death, however due ...
DOI:
10.1016/j.colsurfb.2020.111020