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1. Simultaneous direct determination of 15 glucosinolates in eight Brassica species by UHPLC-Q-Orbitrap-MS

Author:
Hwang, In Min; Park, Boyeon; Dang, Yun Mi; Kim, Su-Yeon; Seo, Hye Young
Source:
Food chemistry 2019 v.282 pp. 127-133
ISSN:
0308-8146
Subject:
broccoli,   etc   ; Brassica; analytical methods; antineoplastic activity; cabbage; cauliflower; glucobrassicin; kale; kimchi; radishes; South Korea;     Show all 11 Subjects
Abstract:
... Glucosinolates (GLS) have been reported to have significant anti-oxidative, antimicrobial, and anti-cancer activities. The current study was aimed to develop an analytical method for glucosinolate quantitation in eight Brassica species from Gwangju, Republic of Korea. For this purpose the UHPLC-Q-Orbitrap-MS technique was used and validated for optimal extraction conditions, detection and quantita ...
DOI:
10.1016/j.foodchem.2018.12.036

2. Analysis of multiple pesticide residues in polyphenol-rich agricultural products by UPLC-MS/MS using a modified QuEChERS extraction and dilution method

Author:
Guo, Jiagang; Tong, Mengmeng; Tang, Jie; Bian, Hongzheng; Wan, Xiaochun; He, Lili; Hou, Ruyan
Source:
Food chemistry 2019 v.274 pp. 452-459
ISSN:
0308-8146
Subject:
broccoli,   etc   ; acephate; acetonitrile; apples; dinotefuran; nitenpyram; omethoate; oolong tea; pesticide residues; polyphenols;     Show all 10 Subjects
Abstract:
... An efficient method for the rapid extraction and clean-up of 20 pesticide residues from polyphenol-rich agricultural samples (tea, apple, broccoli, and shallot) for analysis by UPLC-MS/MS was developed. Following extraction, PVPP was used to precipitate polyphenols, supernatant was diluted to minimize matrix effects. Homogenized samples were vortexed in acetonitrile prior to cleanup with a combina ...
DOI:
10.1016/j.foodchem.2018.08.134

3. Opportunistic Behaviour and Trust: Experimental Results from Broccoli Farmers in Ecuador

Author:
Romero Granja, Cristina; Wollni, Meike
Source:
Journal of agricultural economics 2019 v.70 no.1 pp. 62-80
ISSN:
0021-857X
Subject:
broccoli,   etc   ; data collection; developing countries; exports; farm income; farm tenancy; farmers; markets; supply chain; Ecuador;     Show all 10 Subjects
Abstract:
... Linking smallholder farmers to high‐value markets through contract farming has become an important strategy to increase farm incomes in developing countries. However, various forms of opportunistic behaviour have been reported, threatening the sustainability of inclusive supply chains. When formal institutions are weak, informal codes of conduct like trust can be important complements to facilitat ...
DOI:
10.1111/1477-9552.12271

4. Effects of α‐, β‐ and maltosyl‐β‐cyclodextrins use on the glucoraphanin–sulforaphane system of broccoli juice

Author:
Martínez‐Hernández, Ginés B; Venzke‐Klug, Tâmmila; Carrión‐Monteagudo, María del Mar; Artés Calero, Francisco; López‐Nicolás, José M; Artés‐Hernández, Francisco
Source:
Journal of the science of food and agriculture 2019 v.99 no.2 pp. 941-946
ISSN:
0022-5142
Subject:
broccoli juice,   etc   ; cyclodextrins; flavor; food industry; glucoraphanin; health promotion; industrial applications; processing technology; stabilizers; sulforaphane; thioglucosidase;     Show all 11 Subjects
Abstract:
... Cyclodextrins (CDs) are macromolecules with several industrial applications, being particularly used in the food industry as health‐promoting compounds protection agents, as flavour stabilizers, or to eliminate undesired tastes and browning reactions, among others. This study shows the effects of α‐ (10, 30 and 40 mmol L⁻¹), β‐ (3, 6 and 10 mmol L⁻¹) and maltosyl‐β‐CDs (30, 60 and 90 mmol L⁻¹) use ...
DOI:
10.1002/jsfa.9269

5. Genetically encoded RNA-based sensors for intracellular imaging of silver ions

Author:
YuThese two authors contribute equally to this work., Qikun; Shi, Jing; Mudiyanselage, Aruni P. K. K. Karunanayake; Wu, Rigumula; Zhao, Bin; Zhou, Ming; You, Mingxu
Source:
Chemical communications 2019 v.55 no.5 pp. 707-710
ISSN:
1364-548X
Subject:
broccoli,   etc   ; RNA; antibacterial properties; bacteria; chemical reactions; disinfection; image analysis; ions; oligonucleotides; silver;     Show all 10 Subjects
Abstract:
... Silver has been widely used for disinfection. The cellular accumulation of silver ions (Ag⁺) is critical in these antibacterial effects. The direct cellular measurement of Ag⁺ is useful for the study of disinfection mechanisms. Herein, we reported a novel genetically encoded RNA-based sensor to image Ag⁺ in live bacterial cells. The sensor is designed by introducing a cytosine–Ag⁺–cytosine metallo ...
DOI:
10.1039/c8cc08796b

6. UV-B treatment controls chlorophyll degradation and related gene expression in broccoli (Brassica oleracea L. Italica Group) florets during storage

Author:
Aiamla-or, Sukanya; Shigyo, Masayoshi; Yamauchi, Naoki
Source:
Scientia horticulturae 2019 v.243 pp. 524-527
ISSN:
0304-4238
Subject:
broccoli,   etc   ; Brassica oleracea; chlorophyll; florets; gene expression; gene expression regulation; genes; irradiation; storage temperature; ultraviolet radiation;     Show all 10 Subjects
Abstract:
... Control of chlorophyll (Chl) degradation and related gene expression in response to UV-B treatment were investigated in broccoli florets during storage. Reductions in hue angle value and Chl content in broccoli florets during storage at 4, 15 and 25 °C were delayed with 19 kJ m−2 of UV-B irradiation. Furthermore, up-regulation of BoSGR and BoNYC1, Chl-degrading genes, was found with floret yellowi ...
DOI:
10.1016/j.scienta.2018.09.009

7. Intensifying sulforaphane formation in broccoli sprouts by using other cruciferous sprouts additions

Author:
Liang, Hao; Wei, Yongqin; Li, Ruimin; Cheng, Li; Yuan, Qipeng; Zheng, Fuping
Source:
Food science and biotechnology 2018 v.27 no.4 pp. 957-962
ISSN:
1226-7708
Subject:
broccoli,   etc   ; chemoprevention; glucoraphanin; hydrolysis; radishes; sulforaphane;     Show all 6 Subjects
Abstract:
... Sulforaphane is a significant chemopreventive compound which is the predominant glucosinolate in broccoli sprouts. However, the existence of the epithiospecifier protein could direct the hydrolysis of glucosinolates toward sulforaphane nitrile formation instead of sulforaphane. Therefore, the study aimed on improving the yielding of sulforaphane in broccoli sprouts with a new method of the united ...
DOI:
10.1007/s10068-018-0347-8

8. The effect of chewing on oral glucoraphanin hydrolysis in raw and steamed broccoli

Author:
Sarvan, Irmela; van der Klauw, Michelle; Oliviero, Teresa; Dekker, Matthijs; Verkerk, Ruud
Source:
Journal of functional foods 2018 v.45 pp. 306-312
ISSN:
1756-4646
Subject:
broccoli,   etc   ; glucoraphanin; hydrolysis; mastication; steam; sulforaphane; thioglucosidase;     Show all 7 Subjects
Abstract:
... Chewing disrupts broccoli cells, and myrosinase can effectively hydrolyze the glucosinolate glucoraphanin into the biological active sulforaphane. The influence of chewing time and steaming time on glucoraphanin hydrolysis as well as sulforaphane and sulforaphane nitrile formation in broccoli was studied.To study the effect of chewing time on differently steamed broccoli, broccoli was chewed for 1 ...
DOI:
10.1016/j.jff.2018.04.033

9. Colour, water and chlorophyll loss in harvested broccoli (Brassica oleracea L. Italica) under ambient conditions in Pakistan

Author:
Shakeel, Muhammad; Khan, Salik Nawaz; Saleem, Yasar; Burgess, Paul J.; Shafiq, Shazia
Source:
Scientia horticulturae 2019 v.246 pp. 858-861
ISSN:
0304-4238
Subject:
broccoli,   etc   ; Brassica oleracea; ambient temperature; chemical constituents of plants; chlorophyll; color; florets; relative humidity; storage temperature; turgor; Pakistan;     Show all 11 Subjects
Abstract:
... Broccoli (Brassica oleracea L. Italica) is a nutritious green vegetable containing desirable phytochemicals that is being more widely consumed in Pakistan. However the florets rapidly discolour and wilt after harvest. This study reports the changes in the colour, turgor, weight, visual quality and chlorophyll of harvested broccoli florets after harvest under ambient temperatures of 18 ± 1 °C and a ...
DOI:
10.1016/j.scienta.2018.11.041

10. Effects of hormonal and physical treatments on the expression of a putative chlorophyll b reductase gene (BoNYC1) during postharvest senescence of broccoli

Author:
Reyes Jara, Andrea M.; Gómez-Lobato, María E.; Civello, Pedro M.; Martínez, Gustavo A.
Source:
Postharvest biology and technology 2019 v.147 pp. 107-112
ISSN:
0925-5214
Subject:
broccoli,   etc   ; 1-methylcyclopropene; air; alcohol dehydrogenase; chlorophyll; cytokinins; degreening; ethylene; genes; postharvest treatment; ultraviolet radiation; white light;     Show all 12 Subjects
Abstract:
... Senescence and degreening are the most important features that determine loss of quality during postharvest of broccoli. During senescence, chlorophylls are degraded with the purpose of avoiding presence of photoactive molecules. Chlorophyll b must be previously converted to chlorophyll a in order to be catabolized. This reduction process is catalyzed by two enzymes, chlorophyll b reductase (CBR) ...
DOI:
10.1016/j.postharvbio.2018.09.010

11. Effect of different light-emitting diode (LED) irradiation on the shelf life and phytonutrient content of broccoli (Brassica oleracea L. var. italica)

Author:
Loi, Martina; Liuzzi, Vania C.; Fanelli, Francesca; De Leonardis, Silvana; Maria Creanza, Teresa; Ancona, Nicola; Paciolla, Costantino; Mulè, Giuseppina
Source:
Food chemistry 2019 v.283 pp. 206-214
ISSN:
0308-8146
Subject:
broccoli,   etc   ; ascorbic acid; carotenoids; chlorophyll; cold; cold storage; color; florets; irradiation; light emitting diodes; phenolic compounds; phytonutrients; postharvest storage; proteins; shelf life; Italy;     Show all 16 Subjects
Abstract:
... Broccoli (Brassica oleracea L. var. italica) is largely cultivated in southern Italy. It is an important source of phytonutrients, which are partially lost during postharvest storage. The aim of this work was to evaluate the overall effect of five different low-intensity light-emitting diodes (LEDs) on the quality parameters of broccoli florets over 20 d of cold storage. The level of ascorbic acid ...
DOI:
10.1016/j.foodchem.2019.01.021

12. Reclamation of agro-wastewater polluted with pesticide residues using sunlight activated persulfate for agricultural reuse

Author:
Vela, Nuria; Fenoll, José; Garrido, Isabel; Pérez-Lucas, Gabriel; Flores, Pilar; Hellín, Pilar; Navarro, Simón
Source:
The Science of the total environment 2019 v.660 pp. 923-930
ISSN:
0048-9697
Subject:
broccoli,   etc   ; acetamiprid; acrinathrin; containers; cymoxanil; difenoconazole; flonicamid; folpet; free radicals; hexythiazox; imidacloprid; oxidants; pesticide residues; pymetrozine; sodium; solar radiation; spinosad; spiromesifen; tebuconazole; triadimenol; ultraviolet radiation; washing;     Show all 22 Subjects
Abstract:
... The removal of 17 pesticides (pymetrozine, flonicamid, imidacloprid, acetamiprid, cymoxanil, thiachloprid, spinosad, chlorantraniliprole, triadimenol, tebuconazole, fluopyram, difenoconazole, cyflufenamid, hexythiazox, spiromesifen, folpet and acrinathrin) found in agro-wastewater from washing of containers and phytosanitary treatments equipment, has been carried out using sodium persulfate (Na2S2 ...
DOI:
10.1016/j.scitotenv.2019.01.060

13. Broccoli sprout beverage is safe for thyroid hormonal and autoimmune status: Results of a 12-week randomized trial

Author:
Chartoumpekis, Dionysios V.; Ziros, Panos G.; Chen, Jian-Guo; Groopman, John D.; Kensler, Thomas W.; Sykiotis, Gerasimos P.
Source:
Food and chemical toxicology 2019 v.126 pp. 1-6
ISSN:
0278-6915
Subject:
broccoli,   etc   ; Brassicaceae; autoimmune diseases; autoimmunity; beverages; blood serum; chemoprevention; chronic exposure; drugs; females; glucoraphanin; randomized clinical trials; sulforaphane; thyroglobulin; thyroid function; thyrotropin; thyroxine; toxicity; toxicology; transcription factors;     Show all 20 Subjects
Abstract:
... Sulforaphane is a redox-active natural product present in cruciferous vegetables like broccoli. Broccoli sprout-derived products are promising agents for the prevention of oxidative stress-related diseases, but some have long been suspected of thyroidal toxicity. Recent findings also raise the possibility that long-term exposure to sulforaphane, or to other natural substances or drugs that modulat ...
DOI:
10.1016/j.fct.2019.02.004

14. Evaluation of 2‐Thiothiazolidine‐4‐Carboxylic Acid, a Common Metabolite of Isothiocyanates, as a Potential Biomarker of Cruciferous Vegetable Intake

Author:
Palliyaguru, Dushani L.; Salvatore, Sonia R.; Schopfer, Francisco J.; Cheng, Xuemei; Zhou, Jingyang; Kensler, Thomas W.; Wendell, Stacy G.
Source:
Molecular nutrition & food research 2019 v.63 no.3 pp. e1801029
ISSN:
1613-4125
Subject:
broccoli,   etc   ; Brassicaceae; beverages; bioactive compounds; biomarkers; carboxylic acids; clinical trials; disease prevention; excretion; inflammation; isothiocyanates; metabolites; oxidative stress; powders; rats; vegetable consumption;     Show all 16 Subjects
Abstract:
... SCOPE: Cruciferous vegetable consumption is associated with favorable health outcomes. Bioactive compounds arising in these, especially isothiocyanates, exert effects that contribute to prevention of disease, in large part through the attenuation of inflammation and oxidative stress. However, much about isothiocyanate metabolites and their role as biomarkers of crucifer intake remain unknown. METH ...
DOI:
10.1002/mnfr.201801029

15. Low salinity promotes the growth of broccoli sprouts by regulating hormonal homeostasis and photosynthesis

Author:
Wang, Pei; Li, Xinyue; Tian, Lu; Gu, Zhenxin; Yang, Runqiang
Source:
Horticulture, environment and biotechnology 2019 v.60 no.1 pp. 19-30
ISSN:
2211-3452
Subject:
broccoli,   etc   ; abscisic acid; bioactive compounds; brassinolide; chlorophyll; cytokinins; gibberellic acid; grana; homeostasis; indole acetic acid; leaf area; photosystem II; salinity; seeds; sodium chloride; somatotropin; spraying; ultrastructure;     Show all 18 Subjects
Abstract:
... Broccoli sprouts produce several bioactive compounds and are recognized as a health-promoting vegetable. In this study, the effect of salinity (NaCl) on the growth of broccoli sprouts was investigated. Broccoli seeds were germinated for 4 and 8 d with spraying 0–120 mM NaCl and then harvested to evaluate changes in endogenous hormones, photosynthetic indices, chlorophyll fluorescence parameters an ...
DOI:
10.1007/s13580-018-0095-y

16. Glucoraphanin and sulforaphane evolution during juice preparation from broccoli sprouts

Author:
Bello, Cristiano; Maldini, Mariateresa; Baima, Simona; Scaccini, Cristina; Natella, Fausta
Source:
Food chemistry 2018 v.268 pp. 249-256
ISSN:
0308-8146
Subject:
broccoli,   etc   ; active ingredients; antineoplastic agents; functional foods; glucoraphanin; health promotion; hydrolysis; juices; proteins; sulforaphane;     Show all 10 Subjects
Abstract:
... Broccoli sprouts are considered functional food as they are naturally enriched in glucoraphanin (GR) that is the biological precursor of the anticancer compound sulforaphane (SFN). Due to its health promoting value, also broccoli sprout juice is becoming very popular.The present study aimed to quantitatively assess the conversion of GR to its hydrolysis products, SFN and SFN-nitrile, during the ju ...
DOI:
10.1016/j.foodchem.2018.06.089

17. Transcriptome and DNA methylome reveal insights into yield heterosis in the curds of broccoli (Brassica oleracea L var. italic)

Author:
Li, Hui; Yuan, Jiye; Wu, Mei; Han, Zhanpin; Li, Lihong; Jiang, Hanmin; Jia, Yinglan; Han, Xue; Liu, Min; Sun, Deling; Chen, Chengbin; Song, Wenqin; Wang, Chunguo
Source:
BMC plant biology 2018 v.18 no.1 pp. 168
ISSN:
1471-2229
Subject:
broccoli,   etc   ; DNA methylation; biomass; flowering; genes; heterosis; hybridization; hybrids; intergenic DNA; parents; signal transduction; transcriptome;     Show all 12 Subjects
Abstract:
... BACKGROUND: Curds are the main edible organs, which exhibit remarkable yield heterosis in F₁ hybrid broccoli. However, the molecular basis underlying heterosis in broccoli remains elusive. RESULTS: In the present study, transcriptome profiles revealed that the hybridization made most genes show additive expression patterns in hybrid broccoli. The differentially expressed genes including the non-ad ...
DOI:
10.1186/s12870-018-1384-4

18. 13C quantification in heterogeneous multiphase natural samples by CMP-NMR using stepped decoupling

Author:
Ning, Paris; Soong, Ronald; Bermel, Wolfgang; Lane, Daniel; Simpson, Myrna J.; Simpson, André J.
Source:
Analytical and bioanalytical chemistry 2018 v.410 no.27 pp. 7055-7065
ISSN:
1618-2642
Subject:
broccoli,   etc   ; Arabidopsis; carbon; ethylene; gels; glucose; methanol; nuclear magnetic resonance spectroscopy; soil; stable isotopes;     Show all 10 Subjects
Abstract:
... Many natural and environmental samples contain combinations of liquids, gels, and solids, yet quantification in the intact state and across multiple phases is highly challenging. Comprehensive multiphase nuclear magnetic resonance (CMP-NMR) combines all the capabilities of high-resolution magic angle spinning (HR-MAS), with the addition of full solids power handling, permitting all phases (i.e., m ...
DOI:
10.1007/s00216-018-1306-1

19. Kinetic study of sulforaphane stability in blanched and un-blanched broccoli (Brassica oleracea var. italica) florets during storage at low temperatures

Author:
Mahn, Andrea; Saavedra, Aldo; Paz Rubio, M.
Source:
Journal of food science and technology 2018 v.55 no.11 pp. 4687-4693
ISSN:
0022-1155
Subject:
broccoli,   etc   ; Brassica oleracea var. italica; activation energy; equations; florets; health promotion; models; storage temperature; sulforaphane;     Show all 9 Subjects
Abstract:
... Sulforaphane is a health-promoting compound found in broccoli. Given its high thermo-lability, its preservation through high-temperature processes seems inconvenient. Accordingly, storage at low temperature is an alternative. There are no studies about the evolution of sulforaphane content during storage at low temperatures. The change of sulforaphane content in blanched and un-blanched broccoli f ...
DOI:
10.1007/s13197-018-3395-4

20. Separation and purification of sulforaphane (1-isothiocyanato-4-(methylsulfinyl) butane) from broccoli seeds by consecutive steps of adsorption-desorption-bleaching

Author:
García-Saldaña, Jesús S.; Campas-Baypoli, Olga N.; Sánchez-Machado, Dalia I.; López-Cervantes, Jaime
Source:
Journal of food engineering 2018 v.237 pp. 162-170
ISSN:
0260-8774
Subject:
broccoli,   etc   ; adsorption; bleaching; butanes; desorption; hydrolysis; mass spectrometry; seed extracts; seeds; sulforaphane; ultraviolet-visible spectroscopy;     Show all 11 Subjects
Abstract:
... Sulforaphane has received attention in recent years due to its biological properties; it is found in broccoli seeds but must be released by enzymatic action from its precursor molecule. In this study, a novel process to purification of sulforaphane from broccoli seeds is described. The procedure is divided in three consecutive steps: 1) hydrolysis and extraction, 2) adsorption/desorption of crude ...
DOI:
10.1016/j.jfoodeng.2018.06.002