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1. Improving the efficiency of the CRISPR-Cas12a system with tRNA-crRNA arrays

Author:
Hu, Xixun; Meng, Xiangbing; Li, Jiayang; Wang, Kejian; Yu, Hong
Source:
The crop journal 2020 v.8 no.3 pp. 403-407
ISSN:
2214-5141
Subject:
callus,   etc   ; Oryza sativa; RNA; gene editing; genomics; loci; rice;     Show all 7 Subjects
Abstract:
... CRISPR-Cas12a offers a convenient tool for multiplex genome editing in rice. However, the CRISPR-Cas12a system displays variable editing efficiency across genomic loci, with marked influence by CRISPR RNAs (crRNAs). To improve the efficiency of the CRISPR-Cas12a system for multiplex genome editing, we identified various architectures and expression strategies for crRNAs. Transformation of binary v ...
DOI:
10.1016/j.cj.2019.06.007

2. Callus Induction and Regeneration from Anther Cultures of Indonesian Indica Black Rice Cultivar

Author:
Maharani, Anisa; Fanata, Wahyu Indra Duwi; Laeli, Faida Nur; Kim, Kyung-Min; Handoyo, Tri
Source:
Journal of crop science and biotechnology 2020 v.23 no.1 pp. 21-28
ISSN:
1975-9479
Subject:
callus; callus formation,   etc   ; Oryza sativa; anther culture; black rice; cold; elderly; germplasm; indole acetic acid; kinetin; phenotype; plantlets; putrescine;     Show all 13 Subjects
Abstract:
... The assembly of superior varieties and collection of rice germplasm involves the process of selecting and storing elders that have superior genotypic properties and phenotypes. The anther culture techniques on indica black rice cultivar have a high difficulty factor to get plants, because of the low regeneration ability at the plant formation phase from the anther callus. This study aimed to inves ...
DOI:
10.1007/s12892-019-0322-0

3. Improvement of stilbenoid production by 2-hydroxypropyl-β-cyclodextrin in white mulberry (Morus alba L.) callus cultures

Author:
Komaikul, Jukrapun; Kitisripanya, Tharita; Likhitwitayawuid, Kittisak; Sritularak, Boonchoo; Tanaka, Hiroyuki; Putalun, Waraporn
Source:
Natural product research 2019 v.33 no.19 pp. 2762-2769
ISSN:
1478-6427
Subject:
callus; callus culture,   etc   ; Morus alba; culture media; cyclodextrins; hydrolysis; resveratrol;     Show all 7 Subjects
Abstract:
... Mulberroside A, oxyresveratrol and resveratrol, commonly found in Morus alba L., are potent anti-aging phytostilbenes. In this study, the effect of the addition of 2-hydroxypropyl-β-cyclodextrin on the levels of phytostilbenes in M. alba callus cultures was investigated. Commercial cyclodextrin was used in the hydrolytic and culture processes of the M. alba callus cultures. The hydrolytic study in ...
DOI:
10.1080/14786419.2018.1499643

4. Correction to: Construction of genetic transformation system of Salix mongolica: in vitro leaf-based callus induction, adventitious buds differentiation, and plant regeneration

Author:
Guan, Qingjie; He, Mingliang; Ma, Haiyan; Liao, Xu; Wang, Zhenjuan; Liu, Shenkui
Source:
Plant cell, tissue, and organ culture 2019 v.136 no.1 pp. 205
ISSN:
0167-6857
Subject:
callus,   etc   ; Salix; buds; genetic transformation;     Show all 4 Subjects
Abstract:
... There was an error in the second author’s name in the original article. The name is correctly shown here. ...
DOI:
10.1007/s11240-018-01527-w

5. Proteome analysis of leaf, stem and callus in Viscum album and identification of lectins and viscotoxins with bioactive properties

Author:
Tsekouras, Vasileios; Mavrikou, Sophie; Vlachakis, Dimitrios; Makridakis, Manousos; Stroggilos, Rafael; Zoidakis, Jerome; Termentzi, Aikaterini; Moschopoulou, Georgia; Kintzios, Spyridon
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.1 pp. 167-178
ISSN:
0167-6857
Subject:
callus; callus culture; callus formation,   etc   ; Viscum album; antineoplastic activity; biopharmaceuticals; biosynthesis; chitin; computer simulation; computer software; databases; explants; leaves; lectins; models; neoplasms; parasitic plants; phylogeny; protein content; protein isoforms; protein synthesis; proteome; sequence homology; somaclonal variation;     Show all 24 Subjects
Abstract:
... Mistletoes are semiparasite plants containing pharmaceutical proteins with applications in cancer treatment. Previous research has demonstrated that somaclonal variation can lead to the biosynthesis of novel proteins from mistletoe callus cultures. The protein content of Viscum album subsp. abietis tissues and biotechnologically propagated calluses, was analyzed to identify proteins with putative ...
DOI:
10.1007/s11240-020-01777-7

6. Capacity for somatic embryogenesis of adult oil palm genitors (Elaeis guineensis, var. Pisifera) from immature leaf tissues

Author:
Almeida, Raphael Ferreira; Meira, Filipe Sathler; Gomes, Hugo Teixeira; Balzon, Talita Aparecida; Bartos, Patrícia Monah Cunha; Meira, Rennan de Oliveira; da Cunha, Raimundo Nonato Vieira; Lopes, Ricardo; Mehta, Angela; Scherwinski-Pereira, Jonny Everson
Source:
South African journal of botany 2020 v.131 pp. 229-239
ISSN:
0254-6299
Subject:
callus; callus formation,   etc   ; Elaeis guineensis; activated carbon; adults; biomass; casein; charcoal; explants; genotype; glutamine; growth regulators; hybrids; isopentenyladenine; leaves; males; parents; picloram; somatic embryogenesis; starch granules;     Show all 20 Subjects
Abstract:
... Admittedly, Pisifera genotypes are less responsive to somatic embryogenesis than other genotypes, such as inter- or intra-specific oil palm hybrids. However, clonal propagation of Pisifera plants can be useful for increasing the number of male parents to cross with the Dura variety for mass producing the Tenera commercial varieties. The capacity for somatic embryogenesis (SE) of Brazilian Pisifera ...
DOI:
10.1016/j.sajb.2020.02.026

7. Determination of antioxidant activity, total phenolics and fatty acids in essential oils and other extracts from callus culture, seeds and leaves of Argania spinosa (L.) Skeels

Author:
Koufan, Meriyem; Belkoura, Ilham; Mazri, Mouaad Amine; Amarraque, Abderrahim; Essatte, Amine; Elhorri, Hafsa; Zaddoug, Fatima; Alaoui, Tajlmolk
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.1 pp. 217-227
ISSN:
0167-6857
Subject:
callus; callus culture,   etc   ; Argania spinosa; agroforestry; antioxidant activity; bioactive compounds; cotyledons; dietary supplements; essential oils; explants; fatty acid composition; gallic acid; genotype; leaves; linoleic acid; oleic acid; Morocco;     Show all 17 Subjects
Abstract:
... Argan (Argania spinosa (L.) Skeels) is an endangered and endemic agroforestry species of Morocco highly appreciated for its nutraceutical properties. Herein, the antioxidant activity, total phenolic content and fatty acids were evaluated in different extracts obtained from callus culture, seeds and leaves of four argan genotypes: G25, G36, G41 and G84. Callus induction, proliferation and morpholog ...
DOI:
10.1007/s11240-020-01782-w

8. Effect of salt stress on in vitro organogenesis from nodal explant of Limnophila aromatica (Lamk.) Merr. and Bacopa monnieri (L.) Wettst. and their physio-morphological and biochemical responses

Author:
Dogan, Muhammet
Source:
Physiology and molecular biology of plants 2020 v.26 no.4 pp. 803-816
ISSN:
0971-5894
Subject:
callus; callus formation,   etc   ; Bacopa monnieri; Limnophila; explants; lipid peroxidation; organogenesis; photosynthesis; pigmentation; plant growth; proline; protein content; salinity; salt stress; shoots; tissue culture; toxicity;     Show all 17 Subjects
Abstract:
... Salinity is one of the most severe abiotic stress factors that limit crop productivity by affecting the growth of plants. Therefore, it is significant to know the responses of plants against salt stress. In this study, the callus formation capabilities of nodal explants of Limnophila aromatica (Lamk.) Merr. and Bacopa monnieri (L.) Wettst. incubated under different NaCl concentrations (0–100 mM) i ...
DOI:
10.1007/s12298-020-00798-y
PubMed:
32255941
PubMed Central:
PMC7113342

9. Strategies for indole alkaloids enrichment through callus culture from Alstonia scholaris (L.) R. Br.

Author:
Jeet, Amar; Singh, Yatendra; Singh, Pankaj; Nimoriya, Renu; Bilung, Carol Janis; Kanojiya, Sanjeev; Tripathi, Vineeta; Mishra, Dipak Kumar
Source:
Plant growth regulation 2020 v.90 no.2 pp. 383-392
ISSN:
0167-6903
Subject:
callus; callus culture,   etc   ; 2,4-D; Alstonia scholaris; bromine; carbon; elicitors; high performance liquid chromatography; indole alkaloids; mass spectrometry; pH; plant growth substances; potassium chloride; quantitative analysis; sucrose;     Show all 15 Subjects
Abstract:
... The present study was aimed to investigate the effect of different media, plant growth regulators, carbon sources, sucrose concentrations and pH of the medium for indole alkaloids (IAs) accumulation in the callus culture of Alstonia scholaris. The optimized conditions for the accumulation of IAs like echitamine, acetylechitamine, tubotaiwine and picrinine were found to be the MS medium supplemente ...
DOI:
10.1007/s10725-019-00570-7

10. Development of Plant Prime-Editing Systems for Precise Genome Editing

Author:
Xu, Rongfang; Li, Juan; Liu, Xiaoshuang; Shan, Tiaofeng; Qin, Ruiying; Wei, Pengcheng
Source:
Plant communications 2020 v.1 no.3 pp. 100043
ISSN:
2590-3462
Subject:
callus,   etc   ; Oryza sativa; RNA; gene editing; genome; genomics; humans; mutants; mutation; rice;     Show all 10 Subjects
Abstract:
... Prime-editing systems have the capability to perform efficient and precise genome editing in human cells. In this study, we first developed a plant prime editor 2 (pPE2) system and test its activity by generating a targeted mutation on an HPT⁻ᴬᵀᴳ reporter in rice. Our results showed that the pPE2 system could induce programmable editing at different genome sites. In transgenic T₀ plants, pPE2-gene ...
DOI:
10.1016/j.xplc.2020.100043

11. Production of verbascoside and its analogues in in vitro cultures of Verbascum thapsus L.

Author:
Temporiti, Marta Elisabetta Eleonora; Frezza, Claudio; Beccaccioli, Marzia; Gelardi, Luca; Bianco, Armandodoriano; Bonina, Francesco Paolo; Nielsen, Erik
Source:
Plant cell, tissue, and organ culture 2020 v.140 no.1 pp. 83-93
ISSN:
0167-6857
Subject:
callus,   etc   ; Verbascum thapsus; culture media; cultured cells; explants; glycosides; leaf extracts; leaves; phytochemicals; tissue culture; verbascoside;     Show all 11 Subjects
Abstract:
... In this work an effective method to start axenic cultures of Verbascum thapsus L. was developed. This allowed to obtain a high percentage of callus generation from leaf explants (around 70%) and to start the cultivation of suspension cultured cells rapidly growing in liquid culture medium. Moreover, the production in these materials of the phenylethanoid glycoside compound verbascoside was verifie ...
DOI:
10.1007/s11240-019-01712-5

12. The modulation of ion homeostasis by silicon in cadmium treated poplar callus cells

Author:
Kučerová, Danica; Labancová, Eva; Vivodová, Zuzana; Kollárová, Karin
Source:
Environmental science and pollution research international 2020 v.27 no.3 pp. 2857-2867
ISSN:
0944-1344
Subject:
callus,   etc   ; Populus; cadmium; homeostasis; nutrient balance; nutrient uptake; nutrients; plasma membrane; principal component analysis; silicon; toxicity;     Show all 11 Subjects
Abstract:
... One of the major reasons why cadmium is toxic in plants is because it disturbs their nutrient balance. The aim of this work is to investigate the effects of cadmium (Cd) and/or silicon (Si) on the nutrient status of poplar callus cells after 3 and after 9 weeks of Cd exposure and to study its possible relationship with the changes in the fresh and dry mass, the plasma membrane integrity, and cadmi ...
DOI:
10.1007/s11356-019-07054-1

13. Validation of micrografting to analyze compatibility, shoot growth, and root formation in micrografts of kiwifruit (Actinidia spp.)

Author:
Bao, Wen-Wu; Zhang, Xiao-Chen; Zhang, A-Lin; Zhao, Lei; Wang, Qiao-Chun; Liu, Zhan-De
Source:
Plant cell, tissue, and organ culture 2020 v.140 no.1 pp. 209-214
ISSN:
0167-6857
Subject:
callus formation,   etc   ; Actinidia; budbreak; cambium; graft compatibility; kiwifruit; micropropagation; rootstocks; scions; vascular bundles;     Show all 10 Subjects
Abstract:
... The present study described a micrografting method for kiwifruits and evaluated graft-compatibility, shoot growth, and root formation and growth of the micrografts. Although time durations of callus formation and initiation of new cambial cells varied with the scion cultivars, vascular bundles were established at about day 21 of micrografting in the four kiwifruit scion cultivars tested. Early cal ...
DOI:
10.1007/s11240-019-01723-2

14. Dynamic Epigenetic Changes during Plant Regeneration

Author:
Lee, Kyounghee; Seo, Pil Joon
Source:
Trends in plant science 2018 v.23 no.3 pp. 235-247
ISSN:
1360-1385
Subject:
callus; callus formation,   etc   ; chromatin; epigenetics; tissue culture;     Show all 5 Subjects
Abstract:
... Plants have the remarkable ability to drive cellular dedifferentiation and regeneration. Changes in epigenetic landscapes accompany the cell fate transition. Notably, modifications of chromatin structure occur primarily during callus formation via an in vitro tissue culture process and, thus, pluripotent callus cells have unique epigenetic signatures. Here, we highlight the latest progress in epig ...
DOI:
10.1016/j.tplants.2017.11.009

15. Phenylpropanoids from Callus Tissue of Ajuga turkestanica

Author:
Eshbakova, K. A.; Zakirova, R. P.; Khasanova, Kh. I.; Bobakulov, Kh. M.; Aisa, H. A.; Sagdullaev, Sh. Sh.; Nosov, A. M.
Source:
Chemistry of natural compounds 2019 v.55 no.1 pp. 28-31
ISSN:
0009-3130
Subject:
callus,   etc   ; Ajuga turkestanica; chemical composition; phenylpropanoids;     Show all 4 Subjects
Abstract:
... Phenylpropanoids 3,4-dihydroxy-β-phenylethoxy-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranosyl-(1→3)-4-O-caffeoyl-β-D-glucopyranoside (1) and 3,4-dihydroxy-β-phenylethoxy-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranosyl-(1→3)-4-O-feruloyl-β-D-glucopyronoside (2) were isolated for the first time during studies of the chemical composition of Ajuga turkestanica (Regel) Briq. callus tissue. ...
DOI:
10.1007/s10600-019-02608-8

16. Activation of anthraquinone biosynthesis in long-cultured callus culture of Rubia cordifolia transformed with the rolA plant oncogene

Author:
Veremeichik, G.N.; Bulgakov, V.P.; Shkryl, Y.N.; Silantieva, S.A.; Makhazen, D.S.; Tchernoded, G.K.; Mischenko, N.P.; Fedoreyev, S.A.; Vasileva, E.A.
Source:
Journal of biotechnology 2019 v.306 pp. 38-46
ISSN:
0168-1656
Subject:
callus; callus culture,   etc   ; DNA-binding domains; Rubia cordifolia; anthraquinones; biosynthesis; biotechnology; oncogenes; papilloma; transfer DNA; viruses;     Show all 11 Subjects
Abstract:
... The RolA protein belongs to the RolB class of plant T-DNA oncogenes, and shares structural similarity with the papilloma virus E2 DNA-binding domain. It has potentially as an inducer of plant secondary metabolism, although its role in biotechnology has yet to be realised. In this investigation, a Rubia cordifolia callus culture transformed with the rolA plant oncogene for more than 10 years was an ...
DOI:
10.1016/j.jbiotec.2019.09.007

17. Alkaloid synthesis is coupled to shoot morphogenesis in Argemone mexicana L. (Papaveraceae) in vitro cultures

Author:
Monforte-González, Miriam; Serrano-Gamboa, J. Germán; Guízar-González, Cecilia; Miranda-Ham, Ma. Lourdes; Vázquez-Flota, Felipe A.
Source:
In vitro cellular & developmental biology 2019 v.55 no.6 pp. 695-701
ISSN:
1054-5476
Subject:
callus; callus culture,   etc   ; Argemone mexicana; berberine; buds; explants; internodes; mature plants; organogenesis; roots; sanguinarine; shoots;     Show all 12 Subjects
Abstract:
... During the induction process of an in vitro callus culture of Argemone mexicana L. (Papaveraceae), the levels of two benzylisoquinoline alkaloids known as berberine and sanguinarine displayed opposing trends. While the berberine levels steadily decreased from the initial explant stage up to the early proliferation of unorganized parenchymatous cell masses, the sanguinarine content increased. Once ...
DOI:
10.1007/s11627-019-10007-5

18. Elicitation of Stevia Glycosides Using Salicylic Acid and Silver Nanoparticles Under Callus Culture

Author:
Golkar, Pooran; Moradi, Mahsa; Garousi, Ghasem Ali
Source:
Sugar tech 2019 v.21 no.4 pp. 569-577
ISSN:
0972-1525
Subject:
callus; callus culture,   etc   ; Stevia; bioactive properties; biosynthesis; glycosides; nanosilver; naphthaleneacetic acid; salicylic acid; stevioside;     Show all 10 Subjects
Abstract:
... Development of biosynthesis of phytochemicals, especially medicinal products, is highly important due to their broad bioactivity properties. In this study, optimization of callus growth was initially carried out using various combinations of plant growth regulators. Callus with the highest fresh weight was produced on Murashige and Skoog (MS) medium containing 1 (mg/l) naphthalene acetic acid + 0. ...
DOI:
10.1007/s12355-018-0655-6

19. Role of reduced nitrogen for induction of embryogenic callus induction and regeneration of plantlets in Abelmoschus esculentus L.

Author:
Rizwan, Hafiz Muhammad; Irshad, Muhammad; He, Bizhu; Liu, Shuang; Lu, Xiaocao; Sun, Yueting; Qiu, Dongliang
Source:
South African journal of botany 2020 v.130 pp. 300-307
ISSN:
0254-6299
Subject:
callus; callus formation,   etc   ; 2,4-D; Abelmoschus esculentus; acclimation; activated carbon; ammonium nitrate; cotyledons; explants; genetic transformation; hypocotyls; indole butyric acid; naphthaleneacetic acid; nitrogen; okra; plantlets; potassium nitrate; rooting; roots; shoots; survival rate; tissue culture; total nitrogen;     Show all 23 Subjects
Abstract:
... Okra (Abelmoschus esculentus L.) is a difficult species to regenerate in vitro because of its recalcitrant nature. Whitish colored ‘non-embryogenic’ callus formation is a major problem in okra tissue culture. In this study, different levels of inorganic–nitrogen (KNO₃ and NH₄NO₃ with 1:2 ratios, 30–60 mM) in MS media with different PGRs combinations were tested to obtain friable and embryogenic ca ...
DOI:
10.1016/j.sajb.2020.01.016

20. Box–Behnken supported development and validation of robust HPTLC method: an application in estimation of punarnavine in leaf, stem, and their callus of Boerhavia diffusa Linn

Author:
Ahmad, Wasim; Husain, Ibraheem; Ahmad, Niyaz; Amir, Mohd; Sarafroz, Md.; Ansari, Mohammad Azam; Zafar, Ameeduz; Ali, Abuzer; Zafar, Rasheeduz; Ashraf, Kamran
Source:
3 Biotech 2020 v.10 no.4 pp. 165
ISSN:
2190-572X
Subject:
callus; callus culture,   etc   ; 2,4-D; Boerhavia diffusa; culture media; detection limit; ethyl acetate; experimental design; formic acid; herbs; indole acetic acid; kinetin; leaves; medicinal plants; medicine; plant extracts; quality control; thin layer chromatography; tissue culture; toluene;     Show all 20 Subjects
Abstract:
... Boerhavia diffusa (BD) Linn. (Nyctaginaceae) is one of the most commonly used herbs in the Indian traditional system of medicine for the urinary disorders. The aim of the current investigation was to carry out initiation, development, and maintenance of BD callus cultures and quantitative estimation of punarnavine in plant and callus extracts. Leaves and stem of BD were used as explant for the tis ...
DOI:
10.1007/s13205-020-2154-1
PubMed:
32206499
PubMed Central:
PMC7065290