U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject "callus" Remove constraint Subject: "callus"
Start Over

Search

6,737  Search Results

« Previous | 1 - 20 of 6,737 |
Number of results to display per page

Search Results

1. A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts

Author:
Moon, Ki-Beom; Park, Ji-Sun; Park, Su-Jin; Lee, Hyo-Jun; Cho, Hye-Sun; Min, Sung-Ran; Park, Youn-Il; Jeon, Jae-Heung; Kim, Hyun-Soon
Source:
Plants 2021 v.10 no.4
ISSN:
2223-7747
Subject:
callus,   etc   ; alginates; potatoes; protoplasts; sucrose;     Show all 5 Subjects
Abstract:
... Both obtaining high-yielding, viable protoplasts and following reliable regeneration protocols are prerequisites for the continuous expansion and development of newly emerging systems involving protoplast utilization. This study determines an efficient process from protoplast isolation to shoot regeneration in vitro. The maximum yield of protoplast extraction, which was 6.36 ± 0.51 × 10⁶ protoplas ...
DOI:
10.3390/plants10040781

2. Enhanced somatic embryogenesis and plantlet regeneration in Cenchrus ciliaris L.

Author:
Shashi,; Bhat, Vishnu
Source:
In vitro cellular & developmental biology 2021 v.57 no.3 pp. 499-509
ISSN:
1054-5476
Subject:
callus; callus formation,   etc   ; 2,4-D; Cenchrus ciliaris; casein hydrolysates; glutamine; ontogeny; organogenesis; plantlets; proline; somatic embryogenesis;     Show all 11 Subjects
Abstract:
... A highly reproducible plant regeneration protocol through somatic embryogenesis and shoot organogenesis has been developed for Cenchrus ciliaris. Three explants (seeds, shoot apices, and immature inflorescences) of four genotypes (IG-3108, IG-718, IG-74, and DBC15-8/32/10) were used for callus induction and plant regeneration. The highest rate of callus formation was found using Murashige and Skoo ...
DOI:
10.1007/s11627-020-10148-y

3. Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds

Author:
Babich, Olga; Sukhikh, Stanislav; Pungin, Artem; Astahova, Lidiya; Chupakhin, Evgeny; Belova, Daria; Prosekov, Alexander; Ivanova, Svetlana
Source:
Plants 2021 v.10 no.5
ISSN:
2223-7747
Subject:
callus; callus culture,   etc   ; Hyssopus officinalis; antioxidant activity; culture media; flavonoids; kinetin; naphthaleneacetic acid; saponins; secondary metabolites;     Show all 10 Subjects
Abstract:
... The cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of Hyssopus officinalis were chosen for study. Nutrient media of various compositions were used for Hyssopus officinalis callus culture. For each culture, data on the quant ...
DOI:
10.3390/plants10050915

4. Auxin and cytokinin mediated regulation involved in vitro organogenesis of papaya

Author:
Zhao, Xiaobing; Song, Jinjin; Zeng, Qiuxia; Ma, Yaying; Fang, Hanmei; Yang, Liyuan; Deng, Ban; Liu, Juan; Fang, Jingping; Zuo, Liping; Yue, Jingjing
Source:
Journal of plant physiology 2021 v.260 pp. 153405
ISSN:
0176-1617
Subject:
callus; callus formation,   etc   ; Arabidopsis thaliana; Carica papaya; auxins; cytokinins; gene expression regulation; organogenesis; plant hormones; transcriptome;     Show all 10 Subjects
Abstract:
... In vitro organogenesis is a multistep process which is largely controlled by the balance between auxin and cytokinin. Previous studies revealed a complex network regulating in vitro organogenesis in Arabidopsis thaliana; however, our knowledge of the molecular mechanisms underlying de novo shoot formation in papaya (Carica papaya) remains limited. Here, we optimized multiple factors to achieve an ...
DOI:
10.1016/j.jplph.2021.153405

5. Improved Anther Culture Media for Enhanced Callus Formation and Plant Regeneration in Rice (Oryza sativa L.)

Author:
Ali, Jauhar; Nicolas, Katrina Leslie C.; Akther, Shahana; Torabi, Azerkhsh; Ebadi, Ali Akbar; Marfori-Nazarea, Corinne M.; Mahender, Anumalla
Source:
Plants 2021 v.10 no.5
ISSN:
2223-7747
Subject:
callus; callus formation,   etc   ; Oryza sativa; albino; anther culture; culture media; doubled haploids; homozygosity; plantlets; rice;     Show all 10 Subjects
Abstract:
... Anther culture technique is the most viable and efficient method of producing homozygous doubled haploid plants within a short period. However, the practical application of this technology in rice improvement is still limited by various factors that influence culture efficiency. The present study was conducted to determine the effects of two improved anther culture media, Ali-1 (A1) and Ali-2 (A2) ...
DOI:
10.3390/plants10050839

6. Characterization of LEA genes in Dendrobium officinale and one Gene in induction of callus

Author:
He, Chunmei; Liu, Xuncheng; Teixeira da Silva, Jaime A.; Wang, Haobin; Peng, Tao; Zhang, Mingze; Si, Can; Yu, Zhenming; Tan, Jianwen; Zhang, Jianxia; Luo, Jianping; Duan, Jun
Source:
Journal of plant physiology 2021 v.258-259 pp. 153356
ISSN:
0176-1617
Subject:
callus; callus formation,   etc   ; Arabidopsis; Dendrobium officinale; Phalaenopsis; abscisic acid; cytoplasm; dehydrins; embryogenesis; genes; phylogeny; salt stress;     Show all 12 Subjects
Abstract:
... Late embryogenesis abundant (LEA) proteins are widely involved in plant stress responsive, while their involvement in callus formation is largest unknown. In this study, we identified and conducted expression analysis of the LEA genes from Phalaenopsis equestris and Dendrobium officinale, and characterized a LEA gene from D. officinale. A total 57 and 59 LEA genes were identified in P. equestris a ...
DOI:
10.1016/j.jplph.2020.153356

7. DKW basal salts improve micropropagation and callogenesis compared with MS basal salts in multiple commercial cultivars of Cannabis sativa

Author:
Page, Serena R.G.; Monthony, Adrian S.; Jones, A. Maxwell P.
Source:
Botany 2021 v.99 no.5 pp. 269-279
ISSN:
1916-2804
Subject:
callus; callus formation,   etc   ; 2,4-D; Cannabis sativa; canopy; cultivars; micropropagation; mortality; thidiazuron;     Show all 9 Subjects
Abstract:
... Existing Cannabis sativa micropropagation protocols use a limited number of cultivars and the results are often not reproducible. Currently, Murashige and Skoog basal salt mixture (MS) + 0.5 μmol/L thidiazuron (TDZ) has been reported as the optimal medium for nodal micropropagation, yet our preliminary studies with this medium have resulted in abnormal morphology and high mortality rates in multip ...
DOI:
10.1139/cjb-2020-0179

8. Development of Sinningia magnifica (Otto & A. Dietr.) Wiehler (Gesneriaceae) tissue culture for in vitro production of quinones and bioactive molecules

Author:
Serain, A.F.; Silvério, S.E.B.; De Lourenço, C.C.; Nunes, V.K.; Corrêa, W.R.; Stefanello, M.E.A.; Salvador, M.J.
Source:
Industrial crops and products 2021 v.159 pp. 113046
ISSN:
0926-6690
Subject:
callus; callus culture; callus formation,   etc   ; 2,4-D; Sinningia; antioxidant activity; antioxidants; benzyladenine; culture media; glycosides; metabolism; quinones; secondary metabolites; tissue culture;     Show all 14 Subjects
Abstract:
... Quinones and phenolic glycosides display several biological activities and has been reported in Sinningia species (Gesneriaceae family). The plant tissue culture technique is an excellent tool for metabolism process studies and metabolites extraction. The aim of this work was the callogenesis induction and the secondary callus culture optimization in Sinningia magnifica (Otto & A. Dietr.) Wiehler, ...
DOI:
10.1016/j.indcrop.2020.113046

9. Comparative Anatomy of Cypselae in the Complex Group Chrysolaena, Echinocoryne, Lepidaploa, and Lessingianthus: Contributions to the Systematics of Vernonieae (Compositae)

Author:
Marques, Danilo; de Oliveira Franca, Rafael; Angulo, María Betiana; do Pico, Gisela Mariel Via; Dematteis, Massimiliano; Marzinek, Juliana
Source:
Systematic botany 2020 v.45 no.3 pp. 668-680
ISSN:
0363-6445
Subject:
callus,   etc   ; Vernonia; cypsela; pericarp;     Show all 4 Subjects
Abstract:
... Circumscription of genera in the tribe Vernonieae is complex due to overlapping morphological features among genera such as Chrysolaena, Echinocoryne, Lepidaploa, and Lessingianthus. These genera have been segregated from the genus Vernonia s. l., but their classification remains questionable because they lack clear diagnostic characters to contribute to this classification. Several studies have d ...
DOI:
10.1600/036364420X15935294613545

10. The MdHY5-MdWRKY41-MdMYB transcription factor cascade regulates the anthocyanin and proanthocyanidin biosynthesis in red-fleshed apple

Author:
Mao, Zuolin; Jiang, Huiyan; Wang, Shuo; Wang, Yicheng; Yu, Lei; Zou, Qi; Liu, Wenjun; Jiang, Shenghui; Wang, Nan; Zhang, Zongying; Chen, Xuesen
Source:
Plant science 2021 v.306 pp. 110848
ISSN:
0168-9452
Subject:
callus,   etc   ; anthocyanins; apples; biosynthesis; color; fruits; proanthocyanidins; transcription factors;     Show all 8 Subjects
Abstract:
... Red-fleshed apple fruits are popular because of their high flavonoid content. Although MdMYB10 and its homologs have been identified as crucial regulators of the fruit coloring process, other transcription factors (TFs) contributing to the differences in flesh coloration have not been fully characterized. In this study, we investigated the regulatory effects of MdWRKY41 on anthocyanin and proantho ...
DOI:
10.1016/j.plantsci.2021.110848

11. Seasonal variability and propagation environment to graft success and growth of sapling in tropical and subtropical fruit crops: A review

Author:
Bhandari, Nirajan
Source:
Agricultural reviews 2021 v.42 no.1 pp. 58-65
ISSN:
0976-0741
Subject:
callus formation,   etc   ; developmental stages; fruits; rootstocks; scions; seasonal variation;     Show all 6 Subjects
Abstract:
... Grafting is a widely used propagation technique in fruit trees. Anatomical proximity between scion and rootstock is the prerequisite for the successful graft-take and survival of the grafted plants. The success of grafting, subsequent growth of scion shoot and development of the successful grafts depend upon several factors like climatic conditions, the growth stage of scion and rootstock, methods ...
DOI:
10.18805/ag.R-161

12. Bio nanoparticles as elicitors increase accumulation of betulin and betulinic acid in callus cultures

Author:
Vahide, Payamnoor; Negar, Khodadadi; Hajati Razieh, Jafari
Source:
South African journal of botany 2021 v.141 pp. 431-439
ISSN:
0254-6299
Subject:
callus; callus formation,   etc   ; 2,4-D; Betula pendula; antioxidant activity; bark; betulin; betulinic acid; cellulose; chitin; chitosan; high performance liquid chromatography;     Show all 12 Subjects
Abstract:
... This study aimed to develop a protocol for callus induction and also to evaluate the effects of chitosan nano-fiber (CTS-N), chitin nano-fiber (CT-N), and cellulose nano-fiber (Cellul-N) as elicitors on betulin and betulinic acid production alterations in the produced stem cells of a hybrid of Betula pendula. Inner birch bark samples were cultured in three different WPM, NT, and B5 media applying ...
DOI:
10.1016/j.sajb.2021.05.005

13. In vitro plant regeneration of Christmas cactus (Schlumbergera truncata (Haw.) Moran) by indirect morphogenesis

Author:
Chornobrov, Oksana; Bilous, Svitlana
Source:
Folia forestalia Polonica 2021 v.63 no.1 pp. 68-73
ISSN:
2199-5907
Subject:
callus; callus formation,   etc   ; Schlumbergera truncata; benzyladenine; cacti and succulents; culture media; kinetin; light intensity; lighting; morphogenesis; naphthaleneacetic acid; pigmentation; rooting;     Show all 13 Subjects
Abstract:
... Plants of Schlumbergera truncata (Haw.) Moran were obtained by indirect morphogenesis from the segment section of shoots in vitro, they were multiplied and rooted. Also were determined the effect of the lighting regime, the composition of the nutrient medium on the consistency and frequency of callus formation. The studies were conducted during 2016–2018. The mode of effective sterilization (more ...
DOI:
10.2478/ffp-2021-0007

14. In vitro tissue culture protocol of ancient einkorn (Triticum monococcum ssp. monococum) wheat via indirect shoot regeneration

Author:
Örgeç, Mehmet; Verma, Sandeep Kumar; Şahin, Günce; Zencirci, Nusret; Gürel, Ekrem
Source:
In vitro cellular & developmental biology 2021 v.57 no.1 pp. 143-151
ISSN:
1054-5476
Subject:
callus; callus formation,   etc   ; 2,4-D; Triticum monococcum subsp. monococcum; coleoptiles; indole acetic acid; leaves; rooting; thidiazuron; tissue culture; wheat;     Show all 11 Subjects
Abstract:
... Einkorn indirect shoot regeneration was obtained from leaf, coleoptile, and root explants cultured on full, ½, and ¼ strength Murashige and Skoog (MS) medium containing 0.5 to 10 mg L⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D). Calluses derived from coleoptile and root explants were cultured on full-strength MS medium supplemented with 0.5 to 5 mg L⁻¹ thidiazuron (TDZ) for 6 wk to induce plant regen ...
DOI:
10.1007/s11627-020-10122-8

15. Efficient callus induction and plant regeneration of Malaysian indica rice MR219 using anther culture

Author:
Rahman, Zuraida Abd; Seman, Zulkifli Ahmad; Othman, Ayu Nazreena; Ab Ghaffar, Mohamad Bahagia; Razak, Shahril Ab; Mohd Yusof, Muhammad Fairuz; Nasir, Khairun Hisam; Ahmad, Khairulmazmi; Chow, Yeow Lit; Subramaniam, Sreeramanan
Source:
Biocatalysis and agricultural biotechnology 2021 v.31 pp. 101865
ISSN:
1878-8181
Subject:
callus; callus formation,   etc   ; 2,4-D; agricultural biotechnology; albino; anther culture; benzyladenine; biocatalysis; kinetin; naphthaleneacetic acid; necrosis; plantlets; rice; somatic embryogenesis;     Show all 14 Subjects
Abstract:
... Rice plant regeneration via anther culture possess several difficulties, these included early anther necrosis and high albinism frequency. In the present study, several biotic and abiotic factors were studied to develop an efficient protocol for the regeneration of Malaysian indica rice MR 219 variety. Callus initiation of anther cultures was evaluated using different N6 media supplemented with 2, ...
DOI:
10.1016/j.bcab.2020.101865

16. Histological assessment of regenerating plants at callus, shoot organogenesis and plantlet stages during the in vitro micropropagation of Asparagus cochinchinensis

Author:
Kim, Yong-Goo; Okello, Denis; Yang, Sungyu; Komakech, Richard; Rahmat, Endang; Kang, Youngmin
Source:
Plant cell, tissue, and organ culture 2021 v.144 no.2 pp. 421-433
ISSN:
0167-6857
Subject:
callus; callus culture; callus formation,   etc   ; Asparagus (Asparagaceae); acclimation; benzyladenine; culture media; germplasm conservation; histology; in vitro regeneration; kinetin; leaves; micropropagation; naphthaleneacetic acid; organogenesis; plantlets; root shoot ratio; root systems; rooting; seed germination; seedlings; shoot tips;     Show all 22 Subjects
Abstract:
... In this study, we developed a repeatable in vitro micropropagation protocol for the medicinal plant Asparagus cochinchinensis, based on indirect organogenesis using leaf segments cut from seedlings of in vitro-germinated seeds. We obtained 85% callus induction from the leaf segments within 4–5 weeks when grown on Murashige and Skoog (MS) medium supplemented with benzylaminopurine (BAP, 1.0 mg/L) a ...
DOI:
10.1007/s11240-020-01967-3

17. Identification of regulatory factors promoting embryogenic callus formation in barley through transcriptome analysis

Author:
Suo, Jingqi; Zhou, Chenlu; Zeng, Zhanghui; Li, Xipu; Bian, Hongwu; Wang, Junhui; Zhu, Muyuan; Han, Ning
Source:
BMC plant biology 2021 v.21 no.1 pp. 145
ISSN:
1471-2229
Subject:
callus; callus formation,   etc   ; barley; epigenetics; gene expression; gene expression regulation; gene ontology; genetic transformation; protein phosphorylation; root meristems; tissue culture; transcription (genetics); transcriptome; transcriptomics;     Show all 14 Subjects
Abstract:
... BACKGROUND: Barley is known to be recalcitrant to tissue culture, which hinders genetic transformation and its biotechnological application. To date, the ideal explant for transformation remains limited to immature embryos; the mechanism underlying embryonic callus formation is elusive. RESULTS: This study aimed to uncover the different transcription regulation pathways between calli formed from i ...
DOI:
10.1186/s12870-021-02922-w

18. Improving sesquiterpenoids production of Sarcandra glabra callus culture

Author:
Li, Li; Li, Shan; Cui, Zhirong; Wang, Yingying; Li, Yongyi; Kong, Lingyi; Luo, Jun
Source:
Industrial crops and products 2021 v.169 pp. 113636
ISSN:
0926-6690
Subject:
callus; callus culture,   etc   ; 2,4-D; Oriental traditional medicine; Sarcandra glabra; benzyladenine; electrospray ionization mass spectrometry; herbal tea; inflammation; methyl jasmonate; research and development; sesquiterpenoids; ultraviolet radiation;     Show all 13 Subjects
Abstract:
... Sarcandra glabra, a traditional Chinese medicine and herbal tea, has benefits from treating inflammation and fractures to curing cancer. The anti-inflammatory properties of sesquiterpenoids (chloranthalactone A, CTA; atractylenolide II, AT-II) have made S. glabra to a great potential for research and development. Here this study developed S. glabra callus culture to circumvent particular needs of ...
DOI:
10.1016/j.indcrop.2021.113636

19. Statistical optimization of in vitro callus induction of wild and cultivated varieties of Mucuna pruriens L. (DC.) using response surface methodology and assessment of L-Dopa biosynthesis

Author:
Tandon, Bhavana; Anand, Uttpal; Alex, Blessymole K.; Kaur, Prabhjot; Nandy, Samapika; Shekhawat, Mahipal S.; Sanyal, Rupa; Pandey, Devendra Kumar; Koshy, Eapen P.; Dey, Abhijit
Source:
Industrial crops and products 2021 v.169 pp. 113626
ISSN:
0926-6690
Subject:
callus; callus formation,   etc   ; 2,4-D; L-dopa; Mucuna pruriens; biomass production; biosynthesis; cell culture; chromatography; culture media; drugs; indole acetic acid; juveniles; leaves; response surface methodology; tissue culture;     Show all 16 Subjects
Abstract:
... Mucuna pruriens L. (DC.), is a valuable, economically significant medicinal crop, known worldwide for producing the anti-Parkinson's drug Levodopa (L-Dopa). Itching property of the plant poses certain constrains in its conventional breeding, but, in vitro cell culture systems offer advantage in which controlled conditions can be used for the production of valuable compounds. In the current investi ...
DOI:
10.1016/j.indcrop.2021.113626

20. Establishment of callus and cell suspension cultures of Granny Smith apple fruit and antityrosinase activity of their extracts

Author:
Menbari, Arash; Bahramnejad, Bahman; Abuzaripoor, Morteza; Shahmansouri, Erfan; Zarei, Mohammad Ali
Source:
Scientia horticulturae 2021 v.286 pp. 110222
ISSN:
0304-4238
Subject:
callus; callus formation,   etc   ; 2,4-D; anti-aging properties; apples; caffeic acid; catechin; cell viability; chlorogenic acid; fluorescence microscopy; fruits; inhibitory concentration 50; kojic acid; plant growth; quercetin;     Show all 15 Subjects
Abstract:
... The purpose of the present study was to induce callus and suspension cell cultures from the fruit of Granny Smith apple to measure some of the phenolic compounds in cell culture and evaluate the skin anti-aging effect through measuring the anti-tyrosinase activity. For callus induction MS medium supplemented with different concentrations of plant growth regulators, 2,4-D + BA and 2,4-D + Kin were ...
DOI:
10.1016/j.scienta.2021.110222