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... Brunfelsia is an important genus of the Solanaceae family due to its ornamental aspects and pharmacological properties. Brunfelsia pauciflora stem cuttings represent an alternative to seedling production for its propagation. There is no scientific data currently available concerning B. pauciflora cuttings, although previous studies with Brunfelsia uniflora indicated that it may be difficult to roo ...
... Soil pollution caused by cadmium (Cd) is a serious concern. Phytoremediation is a popular technology in the remediation of Cd-contaminated soil. Salix matsudana var. matsudana f. umbraculifera Rehd. has been characterized as a high Cd-accumulating and tolerant willow (HCW). Here, transcriptome and proteome profiling, along with morphology analyses were performed to explore molecular cross-talk inv ...
callus; callus culture; callus formation, etc ; Capparis spinosa; benzyladenine; chlorogenic acid; methyl isothiocyanate; naphthaleneacetic acid; organ culture; phenolic compounds; quercetin; rutin; strigolactones; sulfur; Show all 14 Subjects
Abstract:
... The effect of strigolactones on plants, which has been recently described as a new group of plant hormones, has not been fully characterized. Capparis spinosa L. callus formation using synthetic strigolactone GR24 (0.1 and 0.2 μM) alone or combined with 1-naphthalene acetic acid (NAA) (10.74 μM) and 6-benzylaminopurine (BAP) (4.44 μM) and its effect on phenolic compounds production were evaluated. ...
callus; callus culture, etc ; 2,4-D; Lactuca; caffeic acid; chlorogenic acid; neural networks; plant growth; Show all 8 Subjects
Abstract:
... The present study aims to model and optimize phenolic acid production from Lactuca undulate Ledeb. root- and leaf-derived callus using the feed-forward artificial neural network (ANN) model. For this purpose, the effect of different concentrations (0, 0.1, 0.5, 1, and 2 mg L⁻¹) of Kin in combinations with or without 2,4-D and or NAA was investigated on callus induction and phenolic acids productio ...
... To reach an efficient and economical gas-phase bioreactor is still one of the most critical challenges in biotechnology engineering. The numerous advantages of gas-phase bioreactors (GPBs) as well as disadvantages of these bioreactors should be exactly recognized, and efforts should be made to eliminate these defects. The first step in upgrading these bioreactors is to identify their types and the ...
... The present study was carried out to optimize in vitro callus induction protocol and whole plant regeneration for developing red rot resistant somaclones in commercial sugarcane variety COC 671 using immature leaf sheaths as explants. In the whole experiment, the best medium for callus induction was MS medium supplemented with 2, 4-D (2.0 mg/l) recorded callus induction frequency of 100 per cent i ...
... Artemisia dracunculus L. (tarragon) is valuable for its medicinal uses such as antimalarial, antibacterial and anticancer. Biotechnological methods need to be developed for the production of phytochemical compounds in plants with high and stable quality. Elicitation is one of the best tools for increasing secondary metabolites in various in vitro cultures. The current study determines the applicat ...
... The study was conducted in six clinical cases of skeletally immature dogs irrespective of breed and sex having transverse or short oblique mid-diaphyseal femoral fractures. In all the animals, fracture was stabilized with elastic stable intramedullary nail using standard titanium elastic nails. Immediate postoperative radiographs showed proper reduction and good alignment of the fractured segments ...
... Allium jesdianum Boiss. & Buhse. is the most important species of the Amaryllidaceae family with various pharmacological properties. Three subsequent experiments (germination, callogenesis, and elicitation) were carried out as a completely randomized design with six replication. At the first study, the highest seed germination (78.33%) was achieved at chemical pre-treatment including the combinati ...
... Fumaria parviflora Lam. is a rare herbaceous annual plant, with a well-known richness of isoquinoline alkaloids. It is threaten due to expansion on construction in the Mediterranean coastal region. We established callus culture protocol aiming at in vitro conservation of this plant. Murashige and Skoog medium fortified with a combination of 0.5 mg/l 1-naphthaleneacetic acid (NAA) and 1 mg/l 6-benz ...
callus; callus culture, etc ; 2,4-D; Rosa chinensis; genetic transformation; germination; kinetin; organ culture; plant growth substances; somatic embryogenesis; Show all 10 Subjects
Abstract:
... Rose is one of the most widely used ornamental flowers in the world. However, the low induction rates for most rose somatic embryos means that it is difficult to use somatic embryos for genetic transformation. In order to improve the induction rate of somatic embryos to improve the genetic transformation efficiency, this study investigated the effects of explant type, plant growth regulator concen ...
callus, etc ; Chrysanthemum; biotechnology; cultivars; kinetin; leaves; Show all 6 Subjects
Abstract:
... To standardize the methodology for in vitro callusing and regeneration of two chrysanthemum cultivars, ‘Candid’ and ‘Flirt,’ an experiment was carried out. The petals and leaf segments were used as explants. In both cultivars, petals were shown to be the most effective for callusing and regeneration. In both cultivars, MS medium enriched with 1.00 mg L⁻¹ BAP + 1.50 mg L⁻¹ NAA resulted in the best ...
Luiza Giacomolli Polesi; Hugo Pacheco de Freitas Fraga; Daniela Goeten; Franklin Panato Back; Eliana de Medeiros Oliveira; Neusa Steiner; Miguel Pedro Guerra
callus; callus formation, etc ; Guadua chacoensis; arginine; bamboos; culture media; etiolation; maltose; mannose; methionine; organ culture; picloram; somatic embryogenesis; spermidine; Show all 14 Subjects
Abstract:
... In this study, we focused on describing and characterizing the somatic embryogenesis (SE) process of Guadua chacoensis through morphohistological and biochemical analysis. For that, G. chacoensis in vitro shoots was used as explant in the induction experiments and we evaluated different Picloram concentrations (0–700 μM), explant pretreatments (constant dark/etiolation or light photoperiod), and e ...
callus; callus formation, etc ; Paulownia; anthocyanins; carotenoids; chemical composition; chlorophyll; culture media; petioles; plantlets; seedlings; somatic embryogenesis; titanium dioxide; trees; China; Show all 15 Subjects
Abstract:
... Paulownia is a fast-growing tree with multiple uses. The present study was conducted to determine the most appropriate concentration of TiO₂ nanoparticles (titanium dioxide) for the induction of somatic embryogenesis and regeneration, reduction of free radicals, and the increase of antioxidant enzymes. Variables examined included explants (leaf and petiole), Paulownia species (Paulownia shantung, ...
callus, etc ; Medicago truncatula; biotechnology; genes; somatic embryogenesis; Show all 5 Subjects
Abstract:
... Somatic embryogenesis is widely used in biotechnology as a regeneration process conditioning many protocols for plant transformation and in vitro propagation. The main objective of this study was to search for new potential somatic embryogenesis regulators among the genes belonging to the NF-Y family and to analyse the mechanism of their action in Medicago truncatula. We found that two LEC1-type N ...
callus; callus formation, etc ; Salvia nemorosa; adenine sulfate; leaves; organogenesis; ornamental plants; plant growth; plantlets; rosmarinic acid; sage; somaclonal variation; woodlands; Show all 13 Subjects
Abstract:
... Salvia nemorosa L. is a valuable medicinal and ornamental plant from Lamiaceae. A high-frequency protocol for in vitro regeneration from S. nemorosa leaf explants was developed based on our results. Various plant growth regulators were evaluated for their effects on direct and indirect organogenesis, callogenesis, shoot multiplication, and rooting of explants at different concentrations. The maxim ...
callus, etc ; altitude; biotechnology; cold stress; genotype; rice; Show all 6 Subjects
Abstract:
... SR4 genotype of rice is high altitude Himalayan rice prone to various abiotic stresses such as cold stress and therefore gives a poor yield. An efficient protocol for callusing and regeneration via direct and indirect means was established using mature seeds as an explant which can be utilized for molecular studies for genetic advancement of Himalayan rice genotype SR4 through transformation. High ...
callus, etc ; Agrobacterium radiobacter; calcium; corn; genetic transformation; plantlets; Show all 6 Subjects
Abstract:
... KEY MESSAGE: We report an optimized transformation system that uses a LaCl₃ pretreatment (a Ca²⁺ channel blocker) for enhancing Agrobacterium-mediated infection of immature embryos and improving the genetic transformation frequency of maize. Agrobacterium-mediated genetic transformation of immature embryos is important for gene-function studies and molecular breeding of maize. However, the relativ ...
callus, etc ; Malus domestica; anthocyanins; apples; metabolism; transcription factors; Show all 6 Subjects
Abstract:
... Anthocyanins play important roles in plant secondary metabolism. Although previous studies have identified many transcription factors (TFs) that participate in the synthetic pathway of anthocyanins, the regulation mechanism of the pathway remain poorly understood. In this study, we identified a WRKY Group IIc TF, MdWRKY75, which contained a typical WRKYGQK heptapeptide sequence and a C2 H2 -zinc f ...
... The bioprocess methodology for the enhanced production of phytochemicals using plant cultures predominantly depends on the elicitors and precursors. The drug L-DOPA (anti-Parkinson's drug) (L-3, 4-dihydroxyphenylalanine) has been chiefly isolated from the popular medicinal plant species Mucuna. The present study has been carried out to enhance the production of L-DOPA and other secondary metabolit ...