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1. Effect of Glucose Starvation on Cell Viability Parameters in A549 and BEAS-2B Cell Lines

Author:
B. G. Çetiner; M. Y. Terzi
Source:
Cytology and genetics 2022 v.56 no.1 pp. 66-76
ISSN:
0095-4527
Subject:
caspase-3,   etc   ; autophagy; cancer therapy; cell viability; glucose; starvation;     Show all 6 Subjects
Abstract:
... Cancer cells are metabolically altered cells characterized by higher glucose uptake. Understanding cancer cells is vital in cancer therapy. We aimed to analyze glucose starvation effect on cell viability parameters in healthy (BEAS-2B) and cancer (A549) cells. We analyzed mTOR, p21, caspase-3, beclin-1, ATG-12 mRNA levels in cells after 8 and 24 h starvations. We also counted senescent cells post- ...
DOI:
10.3103/S0095452722010054

2. Albumin metabolism targeted peptide-drug conjugate strategy for targeting pan-KRAS mutant cancer

Author:
Young Seok Cho; Gui Chul Kim; Hye Min Lee; Byoungmo Kim; Ha Rin Kim; Seung Woo Chung; Hyo Won Chang; Yoon Gun Ko; Yoon Se Lee; Seong Who Kim; Youngro Byun; Sang Yoon Kim
Source:
Journal of controlled release 2022 v.344 pp. 26-38
ISSN:
0168-3659
Subject:
caspase-3,   etc   ; albumins; apoptosis; cytotoxicity; metabolism; mice; mutants; neoplasms; xenotransplantation;     Show all 9 Subjects
Abstract:
... Despite recent breakthroughs in the development of direct KRAS inhibitors and modulators, no drugs targeting pan-KRAS mutant cancers are clinically available. Here, we report a novel strategy to treat pan-KRAS cancers using a caspase-3 cleavable peptide–drug conjugate that exploits enhanced albumin metabolism in KRAS altered cancers to deliver a cytotoxic agent that can induce a widespread bystand ...
DOI:
10.1016/j.jconrel.2022.02.026

3. Plasma membrane perforation by GSDME during apoptosis-driven secondary necrosis

Author:
Elke De Schutter; Jana Ramon; Benjamin Pfeuty; Caroline De Tender; Stephan Stremersch; Koen Raemdonck; Ken Op de Beeck; Wim Declercq; Franck B. Riquet; Kevin Braeckmans; Peter Vandenabeele
Source:
Cellular and molecular life sciences 2022 v.79 no.1 pp. 19
ISSN:
1420-682X
Subject:
caspase-3,   etc   ; apoptosis; dextran; molecular weight; necrosis; phosphatidylserines; plasma membrane;     Show all 7 Subjects
Abstract:
... Secondary necrosis has long been perceived as an uncontrolled process resulting in total lysis of the apoptotic cell. Recently, it was shown that progression of apoptosis to secondary necrosis is regulated by Gasdermin E (GSDME), which requires activation by caspase-3. Although the contribution of GSDME in this context has been attributed to its pore-forming capacity, little is known about the kin ...
DOI:
10.1007/s00018-021-04078-0

4. Cirsiliol mitigates Aβ fibrillation and underlying membrane-leakage associated neurotoxicity: A possible implication in the treatment of neurodegenerative disease

Author:
Hongyu Hu; Wenjun Du; Weidong Zhang; Jun Fang
Source:
International journal of biological macromolecules 2022 v.213 pp. 915-922
ISSN:
0141-8130
Subject:
caspase-3,   etc   ; absorbance; cell viability; fluorescence; humans; neurodegenerative diseases; neurotoxicity; spectroscopy; therapeutics;     Show all 9 Subjects
Abstract:
... Protein aggregating is known as a leading pathogenic characteristic of a wide range of neurodegenerative diseases (NDs). Preventing amyloid-β (Aβ) aggregation and uncovering the associated mechanism through the application of small bioactive compounds can be considered as a useful strategy in hampering the onset of ND. In this study, we analyzed the inhibitory effects of cirsiliol, a trihydroxy-di ...
DOI:
10.1016/j.ijbiomac.2022.06.023

5. Does roundup affect zinc functions in a bivalve mollusk in ex vivo exposure?

Author:
Vira Khoma; Viktoria Martinyuk; Tetyana Matskiv; Kateryna Yunko; Lesya Gnatyshyna; Oksana Stoliar
Source:
Ecotoxicology 2022 v.31 no.2 pp. 335-340
ISSN:
0963-9292
Subject:
caspase-3,   etc   ; Unio; antioxidant activity; chelating agents; ecotoxicology; ethylenediamines; glutathione; glyphosate; zinc;     Show all 9 Subjects
Abstract:
... Roundup (Rn), a glyphosate-based formulation, is one of the most commonly used herbicides in the world. It affects non-targeted organisms in several ways, including adhesive activity towards metal ions. Zinc (Zn) plays a crucial role in a number of biochemical processes. In this study, we aim to elucidate the direct impact of Rn on Zn accumulation and Zn-dependent activities in the ex vivo system. ...
DOI:
10.1007/s10646-021-02512-4

6. Antitumor Effect of Manool in a Murine Melanoma Model

Author:
Heloiza Diniz Nicolella; Arthur Barcelos Ribeiro; Matheus Reis Santos de Melo; Saulo Duarte Ozelin; Lucas Henrique Domingos da Silva; Rodrigo Cássio Sola Veneziani; Denise Crispim Tavares
Source:
Journal of natural products 2022 v.85 no.2 pp. 426-432
ISSN:
1520-6025
Subject:
caspase-3,   etc   ; Salvia officinalis; antineoplastic activity; cisplatin; cytotoxicity; diterpenoids; melanoma; mice; mitosis; models;     Show all 10 Subjects
Abstract:
... The manool diterpene, found in abundance in Salvia officinalis L., showed a selective cytotoxic effect against murine melanoma cells. Therefore, the present study aimed to evaluate the antitumor potential of manool in a murine melanoma model, administered by three routes: oral, subcutaneous, and intraperitoneal. In addition, the antimelanoma effect of manool (orally) combined with cisplatin (subcu ...
DOI:
10.1021/acs.jnatprod.1c01128

7. In Vivo Quantitative Assessment of a Radiation Dose Based on Ratiometric Photoacoustic Imaging of Tumor Apoptosis

Author:
Jing Fang; Yan Zhao; Anna Wang; Yuqi Zhang; Chaoxiang Cui; Shuyue Ye; Qiulian Mao; Yali Feng; Jiachen Li; Chenjie Xu; Haibin Shi
Source:
Analytical chemistry 2022 v.94 no.12 pp. 5149-5158
ISSN:
1520-6882
Subject:
caspase-3,   etc   ; analytical chemistry; apoptosis; fluorescence; fluorescent dyes; neoplasm cells; neoplasms; peptides; radiotherapy;     Show all 9 Subjects
Abstract:
... Accurately assessing the radiation level of tumors and surrounding tissues is of great significance for the optimization of clinical therapeutic interventions as well as minimizing the radiation-induced side effects. Therefore, the development of noninvasive and sensitive biological dosimeters is vital to achieve quantitative detection of a radiation dose in a living system. Herein, as a proof of ...
DOI:
10.1021/acs.analchem.2c00098

8. Polystyrene micro(nano)plastics damage the organelles of RBL-2H3 cells and promote MOAP-1 to induce apoptosis

Author:
Ling Liu; Bingyan Liu; Bowen Zhang; Yiyuan Ye; Wei Jiang
Source:
Journal of hazardous materials 2022 v.438 pp. 129550
ISSN:
0304-3894
Subject:
caspase-3,   etc   ; apoptosis; cytotoxicity; genes; leukemia; microplastics; mitochondria; oxidative stress; polystyrenes; rats; transcriptome;     Show all 11 Subjects
Abstract:
... The ubiquity of microplastics increases the exposure risks and health threats to humans. In this study, rat basophilic leukemia (RBL-2H3) cells were exposed to polystyrene particles (PS-particles) of 50 nm, 500 nm and 5 µm to investigate organelle damage and the mechanism of cell death. PS-particles induced oxidative stress, which in turn led to mitochondrial and lysosomal damage, arrested the cel ...
DOI:
10.1016/j.jhazmat.2022.129550

9. RETRACTED: LINC01606 promotes nephroblastoma by suppressing miR-644b-3p that targets AK4

Author:
Zhen Li; Kui Ai; Wenchang Ni
Source:
Biochemical and biophysical research communications 2022 pp. -
ISSN:
0006-291X
Subject:
caspase-3,   etc   ; RNA; Western blotting; apoptosis; cell proliferation; luciferase; oncogenes; reporter genes; research; therapeutics;     Show all 10 Subjects
Abstract:
... The role of LINC01606 in nephroblastoma progression is unclear. Here, we investigated the underlying mechanism by which LINC01606 regulates nephroblastoma progression.The expression levels of LINC01606, miR-644b-3p, and AK4 were detected using RT-qPCR, and the correlation between them was estimated by Pearson correlation analysis. The effects of LINC01606 knockdown on cell proliferation, apoptosis ...
DOI:
10.1016/j.bbrc.2022.01.005

10. The role of endogenous proteases in degrading grass carp (Ctenopharyngodon idella) myofibrillar structural proteins during ice storage

Author:
Qixing Jiang; Fang Yang; Shengnan Jia; Dawei Yu; Pei Gao; Yanshun Xu; Wenshui Xia
Source:
Lebensmittel-Wissenschaft + Technologie 2022 v.154 pp. 112743
ISSN:
0023-6438
Subject:
caspase-3,   etc   ; Ctenopharyngodon idella; calpain; cathepsin B; cold storage; desmin; fish; texture; troponin T;     Show all 9 Subjects
Abstract:
... Fish texture is important for consumers and degradation of myofibrillar structural proteins could cause softening. However, the role of endogenous proteases in degrading was not clear. In this study, protease inhibitors (Ac-DEVD-CHO, E−64 and EDTA-2Na) were used to distinguish the role of endogenous caspase-3, cathepsin B and L, and calpain. Results showed that caspase-3 and calpain had relatively ...
DOI:
10.1016/j.lwt.2021.112743

11. Aplydactylonins A-C, three new sesquiterpenes from the Vietnamese sea hare Aplysia dactylomela and their cytotoxicity

Author:
Pham Thi Mai Huong; Nguyen Viet Phong; Nguyen Thi Huong; Duong Thu Trang; Do Thi Thao; Nguyen Xuan Cuong; Nguyen Hoai Nam; Nguyen Van Thanh
Source:
Natural medicines 2022 v.76 no.1 pp. 210-219
ISSN:
1340-3443
Subject:
caspase-3,   etc   ; Aplysia; X-ray diffraction; apoptosis; cytotoxicity; density functional theory; sesquiterpenoids; spectral analysis;     Show all 8 Subjects
Abstract:
... Aplydactylonins A-C (1–3), three new sesquiterpenes, were isolated from the Vietnamese sea hare Aplysia dactylomela. Their structures and absolute configurations were elucidated based on spectroscopic analysis, X-ray crystallography, and density functional theory (DFT) calculations of NMR and ECD data. Compound 2 exhibited cytotoxicity against HepG2, DU145 and A549 cells with respective IC₅₀ value ...
DOI:
10.1007/s11418-021-01582-2

12. Investigation on redox status and gene expression related to larval cryopreservation in the Pacific oyster Crassostrea gigas

Author:
Yibing Liu; Xin Zhan; Sarah R. Catalano; Jianguang Qin; Jiabo Han; Xiaoxu Li
Source:
Fisheries science 2022 v.88 no.3 pp. 377-386
ISSN:
0919-9268
Subject:
caspase-3,   etc   ; Crassostrea gigas; antioxidant activity; cryopreservation; gene expression; genes; larvae; reactive oxygen species;     Show all 8 Subjects
Abstract:
... This study evaluated the effects of larval cryopreservation on redox status of post-thaw trochophore larvae and on gene expression in post-thaw trochophore larvae, D-larvae, umbo larvae, eyed larvae and spat, with the aim to understand the effects of cryodamage in the Pacific oyster Crassostrea gigas. The results showed that total antioxidant capacity was significantly higher in the cryopreserved ...
DOI:
10.1007/s12562-022-01594-1

13. Caspase-3 mediated switch therapy of self-triggered and long-acting prodrugs for metastatic TNBC

Author:
Ha Rin Kim; Young Seok Cho; Seung Woo Chung; Jeong Uk Choi; Yoon Gun Ko; Seong Jin Park; Sang Yoon Kim; Youngro Byun
Source:
Journal of controlled release 2022 v.346 pp. 136-147
ISSN:
0168-3659
Subject:
caspase-3,   etc   ; aggression; apoptosis; breast neoplasms; cytotoxicity; doxorubicin; drug therapy; integrins; metastasis; prognosis; relapse;     Show all 11 Subjects
Abstract:
... Triple-negative breast cancer (TNBC) is characterized by its highly heterogeneous microenvironment and propensity for aggressive behavior, both of which represent, along with poor prognosis and high incidence of relapse, the main challenges of curing the disease. Although recent progress in targeted chemotherapy combinations has shown promising outcomes, conventional targeted chemotherapeutic appr ...
DOI:
10.1016/j.jconrel.2022.04.014

14. Intra-tracheal delivery of mesenchymal stem cell-conditioned medium ameliorates pathological changes by inhibiting apoptosis in asthmatic rats

Author:
Rana Keyhanmanesh; Fatemeh Khodamoradi; Reza Rahbarghazi; Afshin Rahbarghazi; Jafar Rezaie; Mahdi Ahmadi; Leila Salimi; Aref Delkhosh
Source:
Molecular biology reports 2022 v.49 no.5 pp. 3721-3728
ISSN:
0301-4851
Subject:
caspase-3,   etc   ; GATA transcription factors; apoptosis; asthma; gene expression; inflammation; males; molecular biology; rats;     Show all 9 Subjects
Abstract:
... BACKGROUND: Asthma, an inflammatory illness of the lungs, remains the most common long-term disease amongst children. This study tried to elaborate the status of apoptosis in asthmatic pulmonary niche after the application of rat mesenchymal stem cells (MSC-CM)-derived secretome. METHODS AND RESULTS: Here, we randomly allocated male Wistar rats into three groups (n = 8); Control animals were intra ...
DOI:
10.1007/s11033-022-07212-8

15. EPR-based in situ enzymatic activity detection of endogenous caspase-3 in apoptosis cell lysates

Author:
Aokun Liu; Jian Kuang; Yemian Zhou; An Xu; Changlin Tian; Lu Yu
Source:
Chemical communications 2022 v.58 no.61 pp. 8528-8531
ISSN:
1364-548X
Subject:
caspase-3,   etc   ; apoptosis; detection limit; electron paramagnetic resonance spectroscopy; enzyme activity; magnetic separation; magnetism; peptides;     Show all 8 Subjects
Abstract:
... Caspase-3 plays a vital role in cell apoptosis and related diseases. The detection and characterization of endogenous active caspase-3 are of immense value not only for mechanism studies of apoptosis but also for the diagnosis and treatment of apoptosis-related diseases. Here, an electron paramagnetic resonance (EPR)-based enzymatic assay was developed for the detection of caspase-3 activity both ...
DOI:
10.1039/d2cc02361j

16. Di-(2-ethyl hexyl) phthalate induced oxidative stress promotes microplastics mediated apoptosis and necroptosis in mice skeletal muscle by inhibiting PI3K/AKT/mTOR pathway

Author:
Xiaojing Liu; Yilei Zhang; Xinyue Sun; Wenyue Zhang; Xu Shi; Shiwen Xu
Source:
Toxicology 2022 v.474 pp. 153226
ISSN:
0300-483X
Subject:
caspase-3,   etc   ; apoptosis; corn oil; diet; microplastics; necroptosis; oxidative stress; phthalates; skeletal muscle; toxicity; toxicology;     Show all 11 Subjects
Abstract:
... The plastic decomposition product microplastics (MPs) and the plastic additive Di (2-ethylhexyl) phthalate (DEHP) in the environment can damage various organs of the organism by inducing oxidative stress. The PI3K/AKT/mTOR signaling pathway participate in toxin-induced apoptosis and necroptosis. However, the effects of DEHP/MPs alone and combined exposure on skeletal muscle cell injury in mice and ...
DOI:
10.1016/j.tox.2022.153226

17. Resina Draconis extract exerts anti‐HCC effects through METTL3‐m6A‐Survivin axis

Author:
Linlin Zhang; Weiwei Ke; Xiangxuan Zhao; Zaiming Lu
Source:
Phytotherapy research 2022 v.36 no.6 pp. 2542-2557
ISSN:
0951-418X
Subject:
caspase-3,   etc   ; apoptosis; ethanol; genes; hepatoma; liver; metastasis; phytotherapy; pro-apoptotic proteins; research; toxicity; xenotransplantation;     Show all 12 Subjects
Abstract:
... Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide. Herbal medicines have become an important treasure reservoir for anti‐HCC drugs because of their high efficiency and low toxicity. Herein, we investigated whether a 75% ethanol extract from Resina Draconis (ERD) exhibited comprehensive anti‐HCC effects both in vivo and in vitro. We revealed that ERD effectively in ...
DOI:
10.1002/ptr.7467

18. Sox9 Promotes Cardiomyocyte Apoptosis After Acute Myocardial Infarction by Promoting miR-223-3p and Inhibiting MEF2C

Author:
Lu Rui; Rui Liu; Huaping Jiang; Kaiyang Liu
Source:
Molecular biotechnology 2022 v.64 no.8 pp. 902-913
ISSN:
1073-6085
Subject:
caspase-3,   etc   ; apoptosis; biotechnology; cardiomyocytes; caspase-9; cell viability; hypoxia; models; myocardial infarction; promoter regions;     Show all 10 Subjects
Abstract:
... Acute myocardial infarction (AMI) is a severe and even fatal cardiovascular disease. The effect of transcription factors on AMI is intensively explored. Our experiment attempts to probe the role of Sox9 in cardiomyocyte apoptosis after AMI. AMI cell model was established in AC16 cells by hypoxia treatment. Cell viability and apoptosis were assessed. Then, the levels of BAX, Bcl-2, Sox9, miR-223-3p ...
DOI:
10.1007/s12033-022-00471-7

19. The mitochondrial protein Sideroflexin 3 (SFXN3) influences neurodegeneration pathways in vivo

Author:
Leire M. Ledahawsky; Maria Eirini Terzenidou; Ruairidh Edwards; Rachel A. Kline; Laura C. Graham; Samantha L. Eaton; Dinja van der Hoorn; Helena Chaytow; Yu‐Ting Huang; Ewout J. N. Groen; Anna A. L. Motyl; Douglas J. Lamont; Kostas Tokatlidis; Thomas M. Wishart; Thomas H. Gillingwater
Source:
FEBS journal 2022 v.289 no.13 pp. 3894-3914
ISSN:
1742-464X
Subject:
caspase-3,   etc   ; Drosophila; Parkinson disease; cell death; mitochondrial membrane; mitochondrial proteins; neurons; proteomics; synapse; therapeutics;     Show all 10 Subjects
Abstract:
... Synapses are a primary pathological target in neurodegenerative diseases. Identifying therapeutic targets at the synapse could delay progression of numerous conditions. The mitochondrial protein SFXN3 is a neuronally enriched protein expressed in synaptic terminals and regulated by key synaptic proteins, including α‐synuclein. We first show that SFXN3 uses the carrier import pathway to insert into ...
DOI:
10.1111/febs.16377

20. The link between vitamin D status and NF-κB-associated renal dysfunction in experimental diabetes mellitus

Author:
Anna Mazanova; Ihor Shymanskyi; Olha Lisakovska; Dmytro Labudzynskyi; Anna Khomenko; Mykola Veliky
Source:
Biochimica et biophysica acta 2022 v.1866 no.7 pp. 130136
ISSN:
0304-4165
Subject:
caspase-3,   etc   ; animals; blood serum; insulin-dependent diabetes mellitus; kidney diseases; kidneys; protein content; streptozotocin;     Show all 8 Subjects
Abstract:
... Type 1 diabetes (T1D) is accompanied by numerous side effects, including renal dysfunction. Mounting evidence suggests that overactivation of nuclear factor ĸB (NF-κB) is one of the key triggers of diabetes-associated chronic kidney disease. Vitamin D₃ is considered as a strong modulator of a number of transcription factors, including NF-κB. The purpose of our study was to assess the contribution ...
DOI:
10.1016/j.bbagen.2022.130136