An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
caspase-3, etc ; autophagy; cancer therapy; cell viability; glucose; starvation; Show all 6 Subjects
Abstract:
... Cancer cells are metabolically altered cells characterized by higher glucose uptake. Understanding cancer cells is vital in cancer therapy. We aimed to analyze glucose starvation effect on cell viability parameters in healthy (BEAS-2B) and cancer (A549) cells. We analyzed mTOR, p21, caspase-3, beclin-1, ATG-12 mRNA levels in cells after 8 and 24 h starvations. We also counted senescent cells post- ...
Young Seok Cho; Gui Chul Kim; Hye Min Lee; Byoungmo Kim; Ha Rin Kim; Seung Woo Chung; Hyo Won Chang; Yoon Gun Ko; Yoon Se Lee; Seong Who Kim; Youngro Byun; Sang Yoon Kim
caspase-3, etc ; albumins; apoptosis; cytotoxicity; metabolism; mice; mutants; neoplasms; xenotransplantation; Show all 9 Subjects
Abstract:
... Despite recent breakthroughs in the development of direct KRAS inhibitors and modulators, no drugs targeting pan-KRAS mutant cancers are clinically available. Here, we report a novel strategy to treat pan-KRAS cancers using a caspase-3 cleavable peptide–drug conjugate that exploits enhanced albumin metabolism in KRAS altered cancers to deliver a cytotoxic agent that can induce a widespread bystand ...
Elke De Schutter; Jana Ramon; Benjamin Pfeuty; Caroline De Tender; Stephan Stremersch; Koen Raemdonck; Ken Op de Beeck; Wim Declercq; Franck B. Riquet; Kevin Braeckmans; Peter Vandenabeele
caspase-3, etc ; apoptosis; dextran; molecular weight; necrosis; phosphatidylserines; plasma membrane; Show all 7 Subjects
Abstract:
... Secondary necrosis has long been perceived as an uncontrolled process resulting in total lysis of the apoptotic cell. Recently, it was shown that progression of apoptosis to secondary necrosis is regulated by Gasdermin E (GSDME), which requires activation by caspase-3. Although the contribution of GSDME in this context has been attributed to its pore-forming capacity, little is known about the kin ...
caspase-3, etc ; absorbance; cell viability; fluorescence; humans; neurodegenerative diseases; neurotoxicity; spectroscopy; therapeutics; Show all 9 Subjects
Abstract:
... Protein aggregating is known as a leading pathogenic characteristic of a wide range of neurodegenerative diseases (NDs). Preventing amyloid-β (Aβ) aggregation and uncovering the associated mechanism through the application of small bioactive compounds can be considered as a useful strategy in hampering the onset of ND. In this study, we analyzed the inhibitory effects of cirsiliol, a trihydroxy-di ...
caspase-3, etc ; Unio; antioxidant activity; chelating agents; ecotoxicology; ethylenediamines; glutathione; glyphosate; zinc; Show all 9 Subjects
Abstract:
... Roundup (Rn), a glyphosate-based formulation, is one of the most commonly used herbicides in the world. It affects non-targeted organisms in several ways, including adhesive activity towards metal ions. Zinc (Zn) plays a crucial role in a number of biochemical processes. In this study, we aim to elucidate the direct impact of Rn on Zn accumulation and Zn-dependent activities in the ex vivo system. ...
Heloiza Diniz Nicolella; Arthur Barcelos Ribeiro; Matheus Reis Santos de Melo; Saulo Duarte Ozelin; Lucas Henrique Domingos da Silva; Rodrigo Cássio Sola Veneziani; Denise Crispim Tavares
caspase-3, etc ; Salvia officinalis; antineoplastic activity; cisplatin; cytotoxicity; diterpenoids; melanoma; mice; mitosis; models; Show all 10 Subjects
Abstract:
... The manool diterpene, found in abundance in Salvia officinalis L., showed a selective cytotoxic effect against murine melanoma cells. Therefore, the present study aimed to evaluate the antitumor potential of manool in a murine melanoma model, administered by three routes: oral, subcutaneous, and intraperitoneal. In addition, the antimelanoma effect of manool (orally) combined with cisplatin (subcu ...
caspase-3, etc ; analytical chemistry; apoptosis; fluorescence; fluorescent dyes; neoplasm cells; neoplasms; peptides; radiotherapy; Show all 9 Subjects
Abstract:
... Accurately assessing the radiation level of tumors and surrounding tissues is of great significance for the optimization of clinical therapeutic interventions as well as minimizing the radiation-induced side effects. Therefore, the development of noninvasive and sensitive biological dosimeters is vital to achieve quantitative detection of a radiation dose in a living system. Herein, as a proof of ...
caspase-3, etc ; apoptosis; cytotoxicity; genes; leukemia; microplastics; mitochondria; oxidative stress; polystyrenes; rats; transcriptome; Show all 11 Subjects
Abstract:
... The ubiquity of microplastics increases the exposure risks and health threats to humans. In this study, rat basophilic leukemia (RBL-2H3) cells were exposed to polystyrene particles (PS-particles) of 50 nm, 500 nm and 5 µm to investigate organelle damage and the mechanism of cell death. PS-particles induced oxidative stress, which in turn led to mitochondrial and lysosomal damage, arrested the cel ...
caspase-3, etc ; RNA; Western blotting; apoptosis; cell proliferation; luciferase; oncogenes; reporter genes; research; therapeutics; Show all 10 Subjects
Abstract:
... The role of LINC01606 in nephroblastoma progression is unclear. Here, we investigated the underlying mechanism by which LINC01606 regulates nephroblastoma progression.The expression levels of LINC01606, miR-644b-3p, and AK4 were detected using RT-qPCR, and the correlation between them was estimated by Pearson correlation analysis. The effects of LINC01606 knockdown on cell proliferation, apoptosis ...
caspase-3, etc ; Ctenopharyngodon idella; calpain; cathepsin B; cold storage; desmin; fish; texture; troponin T; Show all 9 Subjects
Abstract:
... Fish texture is important for consumers and degradation of myofibrillar structural proteins could cause softening. However, the role of endogenous proteases in degrading was not clear. In this study, protease inhibitors (Ac-DEVD-CHO, E−64 and EDTA-2Na) were used to distinguish the role of endogenous caspase-3, cathepsin B and L, and calpain. Results showed that caspase-3 and calpain had relatively ...
caspase-3, etc ; Aplysia; X-ray diffraction; apoptosis; cytotoxicity; density functional theory; sesquiterpenoids; spectral analysis; Show all 8 Subjects
Abstract:
... Aplydactylonins A-C (1–3), three new sesquiterpenes, were isolated from the Vietnamese sea hare Aplysia dactylomela. Their structures and absolute configurations were elucidated based on spectroscopic analysis, X-ray crystallography, and density functional theory (DFT) calculations of NMR and ECD data. Compound 2 exhibited cytotoxicity against HepG2, DU145 and A549 cells with respective IC₅₀ value ...
caspase-3, etc ; Crassostrea gigas; antioxidant activity; cryopreservation; gene expression; genes; larvae; reactive oxygen species; Show all 8 Subjects
Abstract:
... This study evaluated the effects of larval cryopreservation on redox status of post-thaw trochophore larvae and on gene expression in post-thaw trochophore larvae, D-larvae, umbo larvae, eyed larvae and spat, with the aim to understand the effects of cryodamage in the Pacific oyster Crassostrea gigas. The results showed that total antioxidant capacity was significantly higher in the cryopreserved ...
caspase-3, etc ; aggression; apoptosis; breast neoplasms; cytotoxicity; doxorubicin; drug therapy; integrins; metastasis; prognosis; relapse; Show all 11 Subjects
Abstract:
... Triple-negative breast cancer (TNBC) is characterized by its highly heterogeneous microenvironment and propensity for aggressive behavior, both of which represent, along with poor prognosis and high incidence of relapse, the main challenges of curing the disease. Although recent progress in targeted chemotherapy combinations has shown promising outcomes, conventional targeted chemotherapeutic appr ...
caspase-3, etc ; GATA transcription factors; apoptosis; asthma; gene expression; inflammation; males; molecular biology; rats; Show all 9 Subjects
Abstract:
... BACKGROUND: Asthma, an inflammatory illness of the lungs, remains the most common long-term disease amongst children. This study tried to elaborate the status of apoptosis in asthmatic pulmonary niche after the application of rat mesenchymal stem cells (MSC-CM)-derived secretome. METHODS AND RESULTS: Here, we randomly allocated male Wistar rats into three groups (n = 8); Control animals were intra ...
caspase-3, etc ; apoptosis; detection limit; electron paramagnetic resonance spectroscopy; enzyme activity; magnetic separation; magnetism; peptides; Show all 8 Subjects
Abstract:
... Caspase-3 plays a vital role in cell apoptosis and related diseases. The detection and characterization of endogenous active caspase-3 are of immense value not only for mechanism studies of apoptosis but also for the diagnosis and treatment of apoptosis-related diseases. Here, an electron paramagnetic resonance (EPR)-based enzymatic assay was developed for the detection of caspase-3 activity both ...
caspase-3, etc ; apoptosis; corn oil; diet; microplastics; necroptosis; oxidative stress; phthalates; skeletal muscle; toxicity; toxicology; Show all 11 Subjects
Abstract:
... The plastic decomposition product microplastics (MPs) and the plastic additive Di (2-ethylhexyl) phthalate (DEHP) in the environment can damage various organs of the organism by inducing oxidative stress. The PI3K/AKT/mTOR signaling pathway participate in toxin-induced apoptosis and necroptosis. However, the effects of DEHP/MPs alone and combined exposure on skeletal muscle cell injury in mice and ...
caspase-3, etc ; apoptosis; ethanol; genes; hepatoma; liver; metastasis; phytotherapy; pro-apoptotic proteins; research; toxicity; xenotransplantation; Show all 12 Subjects
Abstract:
... Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide. Herbal medicines have become an important treasure reservoir for anti‐HCC drugs because of their high efficiency and low toxicity. Herein, we investigated whether a 75% ethanol extract from Resina Draconis (ERD) exhibited comprehensive anti‐HCC effects both in vivo and in vitro. We revealed that ERD effectively in ...
caspase-3, etc ; apoptosis; biotechnology; cardiomyocytes; caspase-9; cell viability; hypoxia; models; myocardial infarction; promoter regions; Show all 10 Subjects
Abstract:
... Acute myocardial infarction (AMI) is a severe and even fatal cardiovascular disease. The effect of transcription factors on AMI is intensively explored. Our experiment attempts to probe the role of Sox9 in cardiomyocyte apoptosis after AMI. AMI cell model was established in AC16 cells by hypoxia treatment. Cell viability and apoptosis were assessed. Then, the levels of BAX, Bcl-2, Sox9, miR-223-3p ...
Leire M. Ledahawsky; Maria Eirini Terzenidou; Ruairidh Edwards; Rachel A. Kline; Laura C. Graham; Samantha L. Eaton; Dinja van der Hoorn; Helena Chaytow; Yu‐Ting Huang; Ewout J. N. Groen; Anna A. L. Motyl; Douglas J. Lamont; Kostas Tokatlidis; Thomas M. Wishart; Thomas H. Gillingwater
caspase-3, etc ; Drosophila; Parkinson disease; cell death; mitochondrial membrane; mitochondrial proteins; neurons; proteomics; synapse; therapeutics; Show all 10 Subjects
Abstract:
... Synapses are a primary pathological target in neurodegenerative diseases. Identifying therapeutic targets at the synapse could delay progression of numerous conditions. The mitochondrial protein SFXN3 is a neuronally enriched protein expressed in synaptic terminals and regulated by key synaptic proteins, including α‐synuclein. We first show that SFXN3 uses the carrier import pathway to insert into ...
caspase-3, etc ; animals; blood serum; insulin-dependent diabetes mellitus; kidney diseases; kidneys; protein content; streptozotocin; Show all 8 Subjects
Abstract:
... Type 1 diabetes (T1D) is accompanied by numerous side effects, including renal dysfunction. Mounting evidence suggests that overactivation of nuclear factor ĸB (NF-κB) is one of the key triggers of diabetes-associated chronic kidney disease. Vitamin D₃ is considered as a strong modulator of a number of transcription factors, including NF-κB. The purpose of our study was to assess the contribution ...