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... Cardiac patch has been proposed as an alternative therapeutic tissue engineering strategy to treat damaged heart for patients who survive the acute myocardial infarction (MI). To that end, studies have focused on designing an ideal cardiac patch assisting myocardium to repair itself. The engineering of three dimensional (3 D) anisotropic knitted scaffolds could be an efficient and effective strate ...
... BACKGROUND: MicroRNA-1290 (miR-1290) has been reported to be involved in many diseases and play a key role during the development process. However, the role of miR-1290 in atherosclerosis (AS) is still unclear. METHODS AND RESULTS: The current study showed that the expressions of miR-1290 were high in serum of patients with hyperlipidemia. The functional role of miR-1290 were then investigated in ...
celladhesion, etc ; bioactive properties; hydrogen; hydroxyapatite; polymerization; tensile strength; Show all 6 Subjects
Abstract:
... The poly(l-lactide) (PLLA)/hydroxyapatite (HAP) composite scaffold is expected to combine the favorable compatibility and processability of PLLA with the excellent bioactivity and osteoconductivity of HAP. Unfortunately, the poor interfacial bonding between PLLA and HAP leads to a deterioration in mechanical properties. In this study, poly(d-lactide) (PDLA) was grafted onto the surface of HAP nano ...
epithelial celladhesion molecule, etc ; antibodies; dissociation; neoplasms; peptides; research; Show all 6 Subjects
Abstract:
... Epithelial cell adhesion molecule (EpCAM) is one of the critical bio-maker for circulating tumor cells (CTC) detection. For capturing CTC, antibody-antigen-based techniques have mainly been explored. However, the expensiveness and tedious manufacturing process have posed certain limitations for antibody-based techniques for its wide applications in cell capturing. On the other hand, peptides are i ...
celladhesion, etc ; adhesion; blood plasma; erythrocytes; humans; pathophysiology; shear stress; Show all 7 Subjects
Abstract:
... Red blood cell (RBC) adhesion to vascular endothelial cells (EC) is considered a potent effector of circulatory disorders, and its enhancement is implicated in the pathophysiology of numerous conditions, mainly hemoglobinopathies. The actual RBC/EC interaction is determined by both cellular and plasmatic factors, and the differentiation between them is essential for understanding its physiological ...
celladhesion, etc ; adhesion; cell movement; epithelium; fibronectins; integrins; laminin; pseudopodia; Show all 8 Subjects
Abstract:
... Junctional adhesion molecule (JAM)-A is a cell adhesion receptor localized at epithelial cell–cell contacts with enrichment at the tight junctions. Its role during cell–cell contact formation and epithelial barrier formation has intensively been studied. In contrast, its role during collective cell migration is largely unexplored. Here, we show that JAM-A regulates collective cell migration of pol ...
celladhesion, etc ; adhesion; blood serum; cell proliferation; genome; luciferase; neoplasm cells; Show all 7 Subjects
Abstract:
... Methods measuring cell proliferation and adhesion are widely used but each hold limitations. We, therefore, introduce novel methods for measuring cell proliferation and adhesion based on CRISPR-modified cancer cell lines secreting luciferase to the growth media. Using CRISPR genome editing, we generated stable luciferase-secreting LS174T, HCT 116, Caco-2, and PANC-1 cell lines. The modified cells ...
celladhesion, etc ; acrosome; ejaculation; epididymis; exocytosis; integrins; plasma membrane; spermatids; testes; Show all 9 Subjects
Abstract:
... Integrins are transmembrane receptors expressed in all nucleated mammalian cells, critically involved in cell-matrix adhesion and cell-cell interactions that modulate many signalling cascades. It is assumed that integrins also provide essential functions of the reproductive system. In this study, we describe the detailed localization and distribution of αV integrin in the plasma membrane of bull s ...
celladhesion, etc ; adhesion; bone marrow; crystal structure; crystallization; hydroxyapatite; models; roughness; Show all 8 Subjects
Abstract:
... Currently, many researches have developed several strategies to design the surface structures of hydroxyapatite (HA), and have proved that the surface structures are pivotal in guiding the adhesion of bone marrow mesenchymal stem cells (BMSCs) as well as subsequent cellular behaviours. Most of these strategies, such as altering roughness and constructing surface patterning of HA, involve the const ...
celladhesion, etc ; data collection; genomics; haploidy; phenotype; protein structure; signal transduction; vertebrates; Show all 8 Subjects
Abstract:
... BACKGROUND: Amphioxus is a model organism for vertebrate evolutionary research. The significant contrast between morphological phenotypic similarity and high-level genetic polymorphism among amphioxus populations has aroused scientists' attention. Here we resequenced 21 amphioxus genomes to over 100X depth and mapped them to a haploid reference. RESULTS: More than 11.5 million common SNPs were det ...
celladhesion, etc ; bone formation; bones; cartilage; chemical reactions; hydrophilicity; microgels; porosity; tissue repair; Show all 9 Subjects
Abstract:
... Microtissues exhibit great advantages in injecting with minimum invasiveness, mimicking natural tissues, and promoting tissue regeneration. However, very few studies have focused on the construction of osteochondral microtissues that could simultaneously support hyaline-like cartilage and bone tissue regeneration. In this study, chondral microtissues that could favor the formation of hyaline-like ...
celladhesion, etc ; adhesion; complementary DNA; extracellular matrix; ligands; porous media; silica; stem cells; tripeptides; Show all 9 Subjects
Abstract:
... A significant bottleneck in the clinical translation of stem cells remains eliciting the desired stem cell behavior once transplanted in the body. In their natural environment, stem cell fate is regulated by their interaction with extracellular matrix (ECM), mainly through integrin-mediated cell adhesion. 2D biointerfaces that selectively present ECM-derived ligands can be used as valuable tools t ...
celladhesion, etc ; blood; cellular microenvironment; gene expression regulation; hydrogels; nitric oxide; phenotype; smooth muscle; Show all 8 Subjects
Abstract:
... Changes in the stiffness of the cellular microenvironment are involved in many pathological processes of blood vessels. Substrate stiffness has been shown to have extensive effects on vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs). However, the material stiffness of most previously reported in-vitro models is ranging from ~100 kPa to the magnitude of MPa, which does not ...
celladhesion, etc ; biocompatibility; carbon dioxide; cell structures; contact angle; cost effectiveness; fibroblasts; fluorescence; hydrophilicity; mice; Show all 10 Subjects
Abstract:
... Porous poly (lactic acid) (PLA)-based scaffolds have been widely used as a promising product in tissue engineering. However, it is still a challenge to prepare the PLA-based scaffolds with high expansion ratio, good hydrophilicity, and excellent cytocompatibility by a green and cost-effective fabrication approach. Herein, we prepared porous PLA-based scaffolds using carbon dioxide (CO₂) as the phy ...
celladhesion molecules, etc ; Eriocheir sinensis; antimicrobial peptides; arthropods; bioinformatics; exons; gene expression; hemocytes; intestines; phagocytosis; Show all 10 Subjects
Abstract:
... Down syndrome cell adhesion molecule (Dscam), also called hypervariable Dscam (Dscam-hv), is an important player in arthropod alternative splicing that connects neurons and immune regulation, acting as a pathogen-specific recognition molecule. Dscam-hv has two forms: transmembrane (TM) Dscam (mDscam) and soluble Dscam (sDscam). Herein, we investigated two transmembrane variants of mDscam resulting ...
celladhesion, etc ; Escherichia coli; antibiotic resistance; membrane permeability; oxidative stress; plasmids; polymers; polysaccharides; prochloraz; risk; Show all 10 Subjects
Abstract:
... Conjugative plasmid transfer is a major contributor to the spread of antibiotic resistance genes (ARGs). However, the role of conventional fungicides on conjugative plasmid transfer has been neglected. Based on the condition that the increasing use of the combination of nano- and conventional fungicides will lead to combined contamination, the effects of a conventional fungicide prochloraz alone o ...
celladhesion, etc ; Antheraea mylitta; Bombyx mori; biocompatibility; bone formation; bones; models; rats; silk; tissue repair; Show all 10 Subjects
Abstract:
... Non-mulberry silk polymers have a promising future in biomedical applications. However, the dissolution of non-mulberry silk fiber is a still challenge and this poor processability has limited the use of this material. Here, we report a unique protocol to process the Antheraea mylitta (AM) silk fiber. We have shown that the cryo-milling of silk fiber reduces the beta sheet content by more than 10% ...
celladhesion, etc ; antimicrobial properties; biofilm; fluorescence; gentian violet; methicillin-resistant Staphylococcus aureus; microbiology; molecular weight; Show all 8 Subjects
Abstract:
... The emergence of microbial resistance to conventional antibiotics, partially attributed to biofilm formation, has urged for new antimicrobial compounds. Here, we reported two novel low molecular weight (LMW) compounds from Lactiplantibacillus plantarum SJ33 and evaluated their biofilm inhibitory effects on Staphylococcus aureus. The compounds C1 and C2 were purified by RP-HPLC and structurally ide ...
... Two-photon polymerization has recently emerged as a promising technique to fabricate scaffolds for three-dimensional (3D) cell culture and tissue engineering. Here, we combined 3D-printed microscale scaffolds fabricated using two-photon polymerization with a bioactive layer-by-layer film coating. This bioactive coating consists of hyaluronic acid and poly(l-lysine) of controlled stiffness, loaded ...
... Mesenchymal stem cell (MSC) therapy is a promising treatment for various intractable disorders including interstitial cystitis/bladder pain syndrome (IC/BPS). However, an analysis of fundamental characteristics driving in vivo behaviors of transplanted cells has not been performed, causing debates about rational use and efficacy of MSC therapy. Here, we implemented two-photon intravital imaging an ...