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1. A pH-sensitive field-effect transistor for monitoring of cancer cell external acid environment

Author:
Ling Xiao; Kun Li; Binzhu Liu; Jiyuan Tu; Tingxian Li; Yu-Tao Li; Guo-Jun Zhang
Source:
Talanta 2023 v.252 pp. 123764
ISSN:
0039-9140
Subject:
cell culture,   etc   ; acidosis; biocompatibility; culture media; graphene; graphene oxide; hydrogen; metabolism; neoplasm cells; neoplasm progression; pH; phosphates; transistors;     Show all 13 Subjects
Abstract:
... The external acid environment of cancer cells is different from that of normal cells, making a profound impact on cancer progression. Here we report a simple poly-l-lysine-modified graphene field-effect transistor (PLL@G-FET) for in situ monitoring of extracellular acidosis around cancer cells. PLL is a well-known material with good biocompatibility and is rich in amino groups that are sensitive t ...
DOI:
10.1016/j.talanta.2022.123764

2. Cell Culture in Microfluidic Droplets

Author:
Sébastien Sart; Gustave Ronteix; Shreyansh Jain; Gabriel Amselem; Charles N. Baroud
Source:
Chemical reviews 2022 v.122 no.7 pp. 7061-7096
ISSN:
1520-6890
Subject:
cell culture,   etc   ; droplets; immunology;     Show all 3 Subjects
Abstract:
... Cell manipulation in droplets has emerged as one of the great successes of microfluidic technologies, with the development of single-cell screening. However, the droplet format has also served to go beyond single-cell studies, namely by considering the interactions between different cells or between cells and their physical or chemical environment. These studies pose specific challenges linked to ...
DOI:
10.1021/acs.chemrev.1c00666

3. Micro-scaffolds as synthetic cell niches: recent advances and challenges

Author:
Kai Weißenbruch; Enrico D Lemma; Marc Hippler; Martin Bastmeyer
Source:
Current opinion in biotechnology 2022 v.73 pp. 290-299
ISSN:
0958-1669
Subject:
cell culture,   etc   ; artificial cells; biotechnology; hydrogels;     Show all 4 Subjects
Abstract:
... Micro-fabrication and nano-fabrication provide useful approaches to address fundamental biological questions by mimicking the physiological microenvironment in which cells carry out their functions. In particular, 2D patterns and 3D scaffolds obtained via lithography, direct laser writing, and other techniques allow for shaping hydrogels, synthetic polymers and biologically derived materials to cr ...
DOI:
10.1016/j.copbio.2021.08.016

4. Dimensionality-Dependent Mechanical Stretch Regulation of Cell Behavior

Author:
Kun Man; Jiafeng Liu; Khang Minh Phan; Kai Wang; Jung Yeon Lee; Xiankai Sun; Michael Story; Debabrata Saha; Jun Liao; Hamid Sadat; Yong Yang
Source:
ACS applied materials & interfaces 2022 v.14 no.15 pp. 17081-17092
ISSN:
1944-8252
Subject:
cell culture,   etc   ; cell structures; endothelium; homeostasis; humans;     Show all 5 Subjects
Abstract:
... A variety of cells are subject to mechanical stretch in vivo, which plays a critical role in the function and homeostasis of cells, tissues, and organs. Deviations from the physiologically relevant mechanical stretch are often associated with organ dysfunction and various diseases. Although mechanical stretch is provided in some in vitro cell culture models, the effects of stretch dimensionality o ...
DOI:
10.1021/acsami.2c01266

5. Desymmetrization via Activated Esters Enables Rapid Synthesis of Multifunctional Benzene-1,3,5-tricarboxamides and Creation of Supramolecular Hydrogelators

Author:
Shahzad Hafeez; Huey Wen Ooi; Dennis Suylen; Hans Duimel; Tilman M. Hackeng; Clemens van Blitterswijk; Matthew B. Baker
Source:
Journal of the American Chemical Society 2022 v.144 no.9 pp. 4057-4070
ISSN:
1520-5126
Subject:
cell culture,   etc   ; benzene; chondrocytes; hydrogels; hydrophobicity; polymers;     Show all 6 Subjects
Abstract:
... Supramolecular materials based on the self-assembly of benzene-1,3,5-tricarboxamide (BTA) offer an approach to mimic fibrous self-assembled proteins found in numerous natural systems. Yet, synthetic methods to rapidly build complexity, scalability, and multifunctionality into BTA-based materials are needed. The diversity of BTA structures is often hampered by the limited flexibility of existing de ...
DOI:
10.1021/jacs.1c12685

6. Image analyses for engineering advanced tissue biomanufacturing processes

Author:
Mark C. Allenby; Maria A. Woodruff
Source:
Biomaterials 2022 v.284 pp. 121514
ISSN:
0142-9612
Subject:
cell culture,   etc   ; automation; biocompatible materials; bioprocessing; humans;     Show all 5 Subjects
Abstract:
... Industrial cell culture processes are inherently expensive, time-consuming, and variable. These limitations have become a critical bottleneck for the industrial translation of human cell and tissue biomanufacturing, as few human cell culture products deliver sufficient benefit, value, and consistency to offset their high manufacturing costs and produce useful clinical or biomedical solutions. Rece ...
DOI:
10.1016/j.biomaterials.2022.121514

7. Analysis of the aggregation mechanism of chondroitin sulfate/chitosan particles and fabrication of hydrogel cell scaffolds

Author:
Makoto Yamazaki; Kazutoshi Iijima
Source:
International journal of biological macromolecules 2022 v.210 pp. 233-242
ISSN:
0141-8130
Subject:
cell culture,   etc   ; chitosan; chondroitin sulfate; hydrogels; viability;     Show all 5 Subjects
Abstract:
... In this study, the aggregation mechanism of polyion complex (PIC) particles from chitosan (CHI) and chondroitin sulfate C (CS) in phosphate-buffered saline (PBS) was analyzed, and a novel method for the fabrication of hydrogels via aggregation was developed. The PBS induced a decrease in the ζ-potential of the CS/CHI PIC particles, increase in their diameter, and aggregation in a concentration-dep ...
DOI:
10.1016/j.ijbiomac.2022.05.027

8. Dry-compression packing of hydroxyapatite nanoparticles within a flat cuboid chromatography device and its use for fast protein separation

Author:
Raja Ghosh; Geoff Hale; Yves Durocher; Paul Gatt
Source:
Journal of chromatography 2022 v.1667 pp. 462881
ISSN:
0021-9673
Subject:
cell culture,   etc   ; chromatography; hydroxyapatite; mammals; monoclonal antibodies;     Show all 5 Subjects
Abstract:
... We describe and discuss a simple dry-compression technique for preparing a flat cuboid chromatography device containing a shallow packed-bed of crystalline hydroxyapatite nanoparticles. We then discuss the use of this device for fast protein separation in the bind-and-elute mode. Such separation could be carried out at quite low pressures, making it possible to use inexpensive low pressure chromat ...
DOI:
10.1016/j.chroma.2022.462881

9. Tools for studying the cytoskeleton during plant cell division

Author:
Marie-Cécile Caillaud
Source:
Trends in plant science 2022 pp. -
ISSN:
1360-1385
Subject:
cell culture,   etc   ; actin; cell division; microtubules; optogenetics; phenotype;     Show all 6 Subjects
Abstract:
... The plant cytoskeleton regulates fundamental biological processes, including cell division. How to experimentally perturb the cytoskeleton is a key question if one wants to understand the role of both actin filaments (AFs) and microtubules (MTs) in a given biological process. While a myriad of mutants are available, knock-out in cytoskeleton regulators, when nonlethal, often produce little or no p ...
DOI:
10.1016/j.tplants.2022.05.006

10. BoxCar and Library-Free Data-Independent Acquisition Substantially Improve the Depth, Range, and Completeness of Label-Free Quantitative Proteomics

Author:
Devang Mehta; Sabine Scandola; R. Glen Uhrig
Source:
Analytical chemistry 2022 v.94 no.2 pp. 793-802
ISSN:
1520-6882
Subject:
cell culture,   etc   ; Arabidopsis thaliana; analytical chemistry; fractionation; proteomics;     Show all 5 Subjects
Abstract:
... Data-dependent acquisition (DDA) methods are the current standard for quantitative proteomics in many biological systems. However, DDA preferentially measures highly abundant proteins and generates data that is plagued with missing values, requiring extensive imputation. Here, we demonstrate that library-free BoxCarDIA acquisition, combining MS1-level BoxCar acquisition with MS2-level data-indepen ...
DOI:
10.1021/acs.analchem.1c03338

11. Carbonized Silk Nanofibers in Biodegradable, Flexible Temperature Sensors for Extracellular Environments

Author:
Zhan Yang; Ting Huang; Peidong Cao; Yangchen Cui; Jihua Nie; Tao Chen; Hao Yang; Fengxia Wang; Lining Sun
Source:
ACS applied materials & interfaces 2022 v.14 no.16 pp. 18110-18119
ISSN:
1944-8252
Subject:
cell culture,   etc   ; biocompatibility; biodegradability; metabolism; nanofibers; silk; temperature;     Show all 7 Subjects
Abstract:
... Temperature is one of the key parameters for activity of cells. The trade-off between sensitivity and biocompatibility of cell temperature measurement is a challenge for temperature sensor development. Herein, a highly sensitive, biocompatible, and degradable temperature sensor was proposed to detect the living cell extracellular environments. Biocompatible silk materials were applied as sensing a ...
DOI:
10.1021/acsami.2c00384

12. Effects on the crystallization behavior and biocompatibility of poly(LLA-ran-PDO-ran-GA) with poly(d-lactide) as nucleating agents

Author:
Tiantang Fan; Jingwen Qin; Fen Dong; Xiao Meng; Yanqi Li; Ying Wang; Qing Liu; Guannan Wang
Source:
RSC advances 2022 v.12 no.17 pp. 10711-10724
ISSN:
2046-2069
Subject:
cell culture,   etc   ; biocompatibility; crystallization; cytotoxicity; endothermy; thermal degradation;     Show all 6 Subjects
Abstract:
... The blends of poly(l-lactide acid-p-dioxanone-glycolide) (PLPG) with poly(d-lactide) (PDLA) (PLPG/PDLA) were prepared by a solution-casting method. The effects of PDLA on the properties of the PLPG were studied. DSC and WAXD results confirmed that PLA stereocomplex (sc-PLA) crystals were formed by blending PLLA segments in PLPG with PDLA, and the melting endotherm for both PLLA and sc-PLA relied o ...
DOI:
10.1039/d2ra00525e

13. Trends in Droplet Microfluidics: From Droplet Generation to Biomedical Applications

Author:
Zhengkun Chen; Sina Kheiri; Edmond W. K. Young; Eugenia Kumacheva
Source:
Langmuir 2022 v.38 no.20 pp. 6233-6248
ISSN:
1520-5827
Subject:
cell culture,   etc   ; droplets; encapsulation; medicine; microfluidic technology; microgels;     Show all 6 Subjects
Abstract:
... Over the past decade, droplet microfluidics has attracted growing interest in biology, medicine, and engineering. In this feature article, we review the advances in droplet microfluidics, primarily focusing on the research conducted by our group. Starting from the introduction to the mechanisms of microfluidic droplet formation and the strategies for cell encapsulation in droplets, we then focus o ...
DOI:
10.1021/acs.langmuir.2c00491

14. Factors affecting the quality of therapeutic proteins in recombinant Chinese hamster ovary cell culture

Author:
Tae Kwang Ha; Dongil Kim; Che Lin Kim; Lise Marie Grav; Gyun Min Lee
Source:
Biotechnology advances 2022 v.54 pp. 107831
ISSN:
0734-9750
Subject:
cell culture,   etc   ; Cricetulus griseus; biotechnology; glycosylation; protein value; therapeutics;     Show all 6 Subjects
Abstract:
... Chinese hamster ovary (CHO) cells are the most widely used mammalian host cells for the commercial production of therapeutic proteins. Fed-batch culture is widely used to produce therapeutic proteins, including monoclonal antibodies, because of its operational simplicity and high product titer. Despite technical advances in the development of culture media and cell cultures, it is still challengin ...
DOI:
10.1016/j.biotechadv.2021.107831

15. Mammalian viral suppressors of RNA interference

Author:
Wan-Xiang Li; Shou-Wei Ding
Source:
Trends in biochemical sciences 2022 pp. -
ISSN:
0968-0004
Subject:
cell culture,   etc   ; RNA interference; double-stranded RNA; mammals; viruses;     Show all 5 Subjects
Abstract:
... The antiviral defense directed by the RNAi pathway employs distinct specificity and effector mechanisms compared with other immune responses. The specificity of antiviral RNAi is programmed by siRNAs processed from virus-derived double-stranded RNA by Dicer endonuclease. Argonaute-containing RNA-induced silencing complex loaded with the viral siRNAs acts as the effector to mediate specific virus c ...
DOI:
10.1016/j.tibs.2022.05.001

16. Hybrid Models Based on Machine Learning and an Increasing Degree of Process Knowledge: Application to Cell Culture Processes

Author:
Harini Narayanan; Martin Luna; Michael Sokolov; Alessandro Butté; Massimo Morbidelli
Source:
Industrial & engineering chemistry process design and development 2022 v.61 no.25 pp. 8658-8672
ISSN:
1520-5045
Subject:
cell culture,   etc   ; chemistry; hybridization; hybrids; mechanistic models; process design;     Show all 6 Subjects
Abstract:
... In this work, we aim to introduce the concept of the degree of hybridization for cell culture process modeling. We propose that a family of hybrid models can be created with varying fractions of process knowledge explicitly encoded in the model, defined as the degree of hybridization, with the two extremes being fully data-driven (0%) and fully mechanistic (100%) models. Subsequently, the aim is t ...
DOI:
10.1021/acs.iecr.1c04507

17. National Surveillance of Acute Flaccid Paralysis Cases in Senegal during 2017 Uncovers the Circulation of Enterovirus Species A, B and C

Author:
Ndack Ndiaye; Amary Fall; Ousmane Kébé; Davy Kiory; Hamet Dia; Malick Fall; Ndongo Dia; Amadou Alpha Sall; Martin Faye; Ousmane Faye
Source:
Microorganisms 2022 v.10 no.7 pp. -
ISSN:
2076-2607
Subject:
cell culture,   etc   ; Enterovirus C; humans; monitoring; paralysis; phylogeny; Senegal;     Show all 7 Subjects
Abstract:
... Polioviruses have been eliminated in many countries; however, the number of acute flaccid paralysis cases has not decreased. Non-polio enteroviruses are passively monitored as part of the polio surveillance program. Previous studies have shown that some enteroviruses do not grow in conventional cell lines used for the isolation of poliovirus according to the WHO guidelines. In order to evaluate th ...
DOI:
10.3390/microorganisms10071296

18. Thiolated hyaluronic acid/silk fibroin dual-network hydrogel incorporated with bioglass nanoparticles for wound healing

Author:
Yifeng Yu; Bin Yang; Danlei Tian; Jiaoyan Liu; Aixi Yu; Ying Wan
Source:
Carbohydrate polymers 2022 v.288 pp. 119334
ISSN:
0144-8617
Subject:
cell culture,   etc   ; fibroblasts; fibroins; glass; hyaluronic acid; hydrogels; mice;     Show all 7 Subjects
Abstract:
... Thiolated hyaluronic acid(THA) conjugates were synthesized and the selected THA was combined with silk fibroin(SF) and bioactive glass nanoparticles to build a novel type of composite hydrogel with dual-network structure. The optimally achieved gels showed greatly enhanced strength and stiffness compared to the gels built from either THA or SF while having well-defined elasticity and injectability ...
DOI:
10.1016/j.carbpol.2022.119334

19. Laurdan in live cell imaging: Effect of acquisition settings, cell culture conditions and data analysis on generalized polarization measurements

Author:
Sarka Pokorna; Ana E. Ventura; Tânia C.B. Santos; Martin Hof; Manuel Prieto; Anthony H. Futerman; Liana C. Silva
Source:
Journal of photochemistry and photobiology 2022 v.228 pp. 112404
ISSN:
1011-1344
Subject:
cell culture,   etc   ; data quality; fluorescence microscopy; membrane fluidity; photobiology; photochemistry;     Show all 6 Subjects
Abstract:
... Cell function is highly dependent on membrane structure, organization, and fluidity. Therefore, methods to probe the biophysical properties of biological membranes are required. Determination of generalized polarization (GP) values using Laurdan in fluorescence microscopy studies is one of the most widely-used methods to investigate changes in membrane fluidity in vitro and in vivo. In the last co ...
DOI:
10.1016/j.jphotobiol.2022.112404

20. Synthetic scaffolds for 3D cell cultures and organoids: applications in regenerative medicine

Author:
Amanda Marchini; Fabrizio Gelain
Source:
Critical reviews in biotechnology 2022 v.42 no.3 pp. 468-486
ISSN:
1549-7801
Subject:
cell culture,   etc   ; animals; biocompatibility; biocompatible materials; biomimetics; medicine; organoids; peptides;     Show all 8 Subjects
Abstract:
... Three-dimensional (3D) cell cultures offer an unparalleled platform to recreate spatial arrangements of cells in vitro. 3D cell culture systems have gained increasing interest due to their evident advantages in providing more physiologically relevant information and more predictive data compared to their two-dimensional (2D) counterpart. Design and well-established fabrication of organoids (a part ...
DOI:
10.1080/07388551.2021.1932716