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celllines, etc ; Cephalotaxus; cytotoxicity; flavonoids; research; Show all 5 Subjects
Abstract:
... Three new biflavonoids, umcephabiovins C − E (1 − 3), along with fourteen known compounds were isolated from the twigs and leaves of Cephalotaxus oliveri. Their structures and configurations were elucidated by UV, IR, NMR, ECD, and HR-ESI-MS spectra. Compounds 1 − 3 exhibited significant α-glucosidase inhibitory activity with IC₅₀ values of 7.05 ± 2.66, 24.45 ± 4.73, and 1.84 ± 1.14 μM, respective ...
celllines, etc ; data collection; genome; nanopores; telomeres; Show all 5 Subjects
Abstract:
... Despite recent improvements in basecalling accuracy, nanopore sequencing still has higher error rates on short-tandem repeats (STRs). Instead of using basecalled reads, we developed DeepRepeat which converts ionic current signals into red-green-blue channels, thus transforming the repeat detection problem into an image recognition problem. DeepRepeat identifies and accurately quantifies telomeric ...
celllines, etc ; cell membranes; membrane proteins; urea; Show all 4 Subjects
Abstract:
... The capture and detection of cells expressing a breast-cancer related membrane protein, namely a BT474 cell line expressing HER2, is demonstrated using ion-sensitive field effect transistors (ISFETs). BT474 cells were exposed to anti-HER2 antibodies and urease-conjugated secondary antibodies to induce chemical signal amplification by adding urea. ...
celllines, etc ; bioinformatics; luciferase; microRNA; stem cells; Show all 5 Subjects
Abstract:
... Linc-ROR has a regulatory role in reprogramming, and the core stem cell transcription factors, OCT4, SOX2, and NANOG, regulate its expression. MicroRNAs (miRNAs) are also a critical constituent of pivotal posttranscriptional regulatory pathways. One of such interactions is a competing endogenous RNA interaction that connects small and long non-coding RNAs with coding transcripts. Here, we aimed to ...
celllines, etc ; Sertoli cells; coculture; genes; spermatogenesis; testosterone; Show all 6 Subjects
Abstract:
... The Glial cell-derived neurotrophic factor (Gdnf) and testosterone induce the spermatogonial stem cells (SSCs) self-renewal and spermatogenesis, respectively. In present study the stimulating role of testosterone on Sertoli cells to produce Gdnf, and the possible effect of Gdnf on Gfrα1 and c-RET expressions were investigated. The TM4 cells (line Sertoli cells) were co-cultured with [0.1, 0.2 and ...
celllines, etc ; Oroxylum indicum; bark; glycosides; neuroglia; research; Show all 6 Subjects
Abstract:
... Two new phenolic glycosides, oroxylumosides A (1) and B (2), along with four known compounds darendoside A (3), leucosceptoside A (4), acteoside (5) and decaffeoylacteoside (6) were isolated from the stem bark of Oroxylum indicum. Their structures were elucidated by extensive analysis of the 1 D and 2 D NMR as well as HR-ESI-QTOF-MS. In addition, compounds 1 − 4 exhibited inhibitory effects on NO ...
celllines, etc ; humans; interphase; myoblasts; transcription (genetics); transcriptome; Show all 6 Subjects
Abstract:
... Satellite cells are generally quiescent in vivo. Once activated, progression through the cell cycle begins. Immortalised myoblasts from a single cell line are fairly homogenous in culture, but primary human myoblasts (PHMs) demonstrate heterogeneity. This phenomenon is poorly understood however may impact on PHM expansion. This study aimed to evaluate cell cycle transition from growth to synthesis ...
celllines, etc ; Cricetulus griseus; biomarkers; biotechnology; gelatinase B; humans; Show all 6 Subjects
Abstract:
... BACKGROUND: Human matrix metalloproteinase 9 (hMMP9) is a biomarker in several diseases, including cancer, and the need for developing detectors and inhibitors of hMMP9 is increasing. As an antibody against hMMP9 can be selectively bound to hMMP9, the use of anti-MMP9 antibody presents new possibilities to address hMMP9-related diseases. In this study, we aimed to establish a stable Chinese hamste ...
celllines, etc ; cytotoxicity; fibroblasts; new species; secondary metabolites; Iran; Show all 6 Subjects
Abstract:
... Several different techniques were employed for the isolation of Nannocystis from various sources. A polyphasic approach was used for identification. Twelve strains of N. pusilla, N. exedens, and N. konarekensis with distinctive distribution between climates were identified. The bioactivity was examined against a panel of eight bacteria, two yeasts, and one fungus; cytotoxicity was tested on the L9 ...
celllines, etc ; anhydrides; phenylpropanoids; plant biochemistry; spectral analysis; viruses; Show all 6 Subjects
Abstract:
... Using chemical and spectroscopic data, this study on Brachybotrys paridiformis Maxim. ex Oliv. identified four undescribed phenylpropanoids, brachin A–C and brachoside A, together with nine other known compounds. The isolated compounds were tested for anti-hepatitis B virus activities in the HepG2.2.15 cell line. Among them, caffeic anhydride showed the most potent activity. ...
human celllines, etc ; apoptosis; cytotoxicity; flow cytometry; neoplasm cells; Show all 5 Subjects
Abstract:
... In this paper, eight new galaxamide analogues (Z-1~Z-8) were synthesized and evaluated for their cytotoxic activities against five cancer cell lines, MCF-7, MD-MBA-231, HepG2, Hela, and A549, using MTT assays. The modified analogue Z-6 displayed broad spectrum cytotoxic activity toward each tested cell line with IC₅₀ values of 1.65 ± 0.30 (MCF-7), 2.91 ± 0.17 (HepG2), 4.59 ± 0.27 (MD-MBA-231), 5.6 ...
human celllines, etc ; adsorption; analytical chemistry; mass spectrometry; peptides; proteomics; Show all 6 Subjects
Abstract:
... For proteomic analysis based on mass spectrometry (MS), high-performance peptide separation under MS-friendly conditions is of importance. To this end, a novel kind of amine-bridged hybrid monolith was developed by the sol–gel reaction of bis[3-(trimethoxysilyl)propyl]amine and allyltrimethoxysilane, followed by “thiol–ene” click functionalization of C18 groups. With the secondary amino groups bri ...
celllines, etc ; Crotalus durissus; cytotoxicity; neoplasm cells; snake venoms; therapeutics; Show all 6 Subjects
Abstract:
... Snake venoms are natural sources of bioactive substances with therapeutic potential. In this work, we evaluated the cytotoxicity of the Crotalus durissus collilineatus, negative crotamine variety and the isolated fraction C0K3N3 in BALB C/3T3 and K562 cell lines. The results indicate that the C0K3N3 protein is more cytotoxic against the K562 tumor cell line than in the 3T3 baseline. ...
celllines, etc ; astrocytes; bile; genes; immunoassays; isozymes; signal peptide; trypsin; Show all 8 Subjects
Abstract:
... Trypsin has been identified as a pancreatic protease comprising three isoenzymes, trypsin-1, -2, and -3. However, the gene for trypsinogen-3, PRSS3, also gives rise to additional variants, trypsinogen-4A and B, which differ from trypsinogen-3 only with respect to the leader-peptide part, and when activated are identical to trypsin-3. The unique overlapping leader peptides of trypsinogen-4A and B a ...
celllines, etc ; chickens; coculture; epithelial cells; farms; models; organoids; swine; Show all 8 Subjects
Abstract:
... Numerous bioactive plant additives have shown various positive effects in pigs and chickens. The demand for feed additives of natural origin has increased rapidly in recent years to support the health of farm animals and thus minimize the need for antibiotics and other drugs. Although only in vivo experiments can fully represent their effect on the organism, the establishment of reliable in vitro ...
celllines, etc ; forskolin; histamine; mice; pertussis toxin; toxicity; vaccines; Europe; Show all 8 Subjects
Abstract:
... Testing for inactivation of pertussis toxin and reversion to toxicity in aP vaccines has historically relied on the murine histamine sensitization test, that lacks mechanistic understanding, suffers from standardization problems and is associated with severe animal suffering. Though the regulatory requirements for in vivo testing of acellular pertussis (aP) vaccine products have been waived in Eur ...
human celllines, etc ; breast neoplasms; cell proliferation; metastasis; phospholipases; research; Show all 6 Subjects
Abstract:
... Breast cancer stem cells (BCSCs) are slow cycling cells that escape the traditional chemo-radio-therapy, thereby contributing in resistance and recurrence. Although several markers have been identified, it is still challenging to develop strategies targeting them. In this study, we have isolated BCSCs from MCF-7 cell line using markers CD44⁺/CD24⁻/ˡᵒʷ, which showed higher percentage of mammosphere ...
human celllines, etc ; Shuteria; enantiomers; flavanones; neoplasm cells; research; spectral analysis; Show all 7 Subjects
Abstract:
... (±)-Involucrasins A (1) and B (2), two pairs of flavanone enantiomers were isolated from Shuteria involucrata. Structurally, both 1 and 2 are rare representatives of 5-dehydroxy/5-demethoxy 2’,3’,4’-trisubstituted flavanones. Their structures were elucidated on the basis of comprehensive spectroscopic data analysis and comparison with the literature data. Involucrasin B (2) exhibited moderate anti ...
human celllines, etc ; Escherichia coli; adhesion; digestive tract; enteropathogens; intestines; probiotics; Show all 7 Subjects
Abstract:
... The adhesion and aggregation are characteristic attributes of probiotic strains belonging to Lactobacillaceae genus. Due to these properties the host organisms can avoid colonisation of the intestinal tract by enteropathogenic bacteria. The presented research includes a comparison of the properties of various strains belonging to different Lactobacillaceae species and isolated from different sourc ...
Yifan Zhang; Thomas M. Blomquist; Rebecca Kusko; Daniel Stetson; Zhihong Zhang; Lihui Yin; Robert Sebra; Binsheng Gong; Jennifer S. Lococo; Vinay K. Mittal; Natalia Novoradovskaya; Ji-Youn Yeo; Nicole Dominiak; Jennifer Hipp; Amelia Raymond; Fujun Qiu; Hanane Arib; Melissa L. Smith; Jay E. Brock; Daniel H. Farkas; Daniel J. Craig; Erin L. Crawford; Dan Li; Tom Morrison; Nikola Tom; Wenzhong Xiao; Mary Yang; Christopher E. Mason; Todd A. Richmond; Wendell Jones; Donald J. Johann Jr; Leming Shi; Weida Tong; James C. Willey; Joshua Xu
celllines, etc ; DNA; diploidy; formalin; genome; genomics; histology; mutation; patients; therapeutics; Show all 10 Subjects
Abstract:
... BACKGROUND: Clinical laboratories routinely use formalin-fixed paraffin-embedded (FFPE) tissue or cell block cytology samples in oncology panel sequencing to identify mutations that can predict patient response to targeted therapy. To understand the technical error due to FFPE processing, a robustly characterized diploid cell line was used to create FFPE samples with four different pre-tissue proc ...