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1. Novel cascade reaction-based fluorescent cyanine chemosensor for cysteine detection and bioimaging in living system

Author:
Niu, Linqiang; Luo, Yang; Gan, Ying; Cao, Qijuan; Zhu, Cuijuan; Wang, Muxi; Wang, Jiamin; Zhang, Weihong; Wang, Jianhong
Source:
Talanta 2020 v.219 pp. 121291
ISSN:
0039-9140
Subject:
human cell lines,   etc   ; bioimaging; cysteine; fluorescence; mice;     Show all 5 Subjects
Abstract:
... Precise detection of intracellular Cys will be helpful to accurately assess the physiological functions in the physiological and pathological processes. Herein, a new probe Meoeth-Cy-OBz-oCl capable of Cys sensing with high selectivity over other mercaptoamino-acid molecules including Hcy and GSH was developed. The studies on sensing mechanism supported thiols-induced SNAr substitution-rearrangeme ...
DOI:
10.1016/j.talanta.2020.121291

2. Inertial-Force-Assisted, High-Throughput, Droplet-Free, Single-Cell Sampling Coupled with ICP-MS for Real-Time Cell Analysis

Author:
Zhang, Xuan; Wei, Xing; Men, Xue; Jiang, Ze; Ye, Wen-Qi; Chen, Ming-Li; Yang, Ting; Xu, Zhang-Run; Wang, Jian-Hua
Source:
Analytical chemistry 2020 v.92 no.9 pp. 6604-6612
ISSN:
1520-6882
Subject:
human cell lines,   etc   ; antagonism; cadmium; copper;     Show all 4 Subjects
Abstract:
... Single-cell analysis facilitates perception into the most essential processes in life’s mysteries. While it is highly challenging to quantify them at the single-cell level, where precise single-cell sampling is the prerequisite. Herein, a real-time single-cell quantitative platform was established for high-throughput droplet-free single-cell sampling into time-resolved (TRA) ICP-MS and real-time q ...
DOI:
10.1021/acs.analchem.0c00376

3. Shared distal regulatory regions may contribute to the coordinated expression of human ribosomal protein genes

Author:
Wang, Saidi; Hu, Haiyan; Li, Xiaoman
Source:
Genomics 2020 v.112 no.4 pp. 2886-2893
ISSN:
0888-7543
Subject:
cell lines,   etc   ; chromatin; genes; humans; ribosomal proteins; transcription (genetics);     Show all 6 Subjects
Abstract:
... To identify the potential distal regulatory regions of human ribosomal protein genes (RPGs) and to understand their characteristics, we studied the chromatin interactions in seven cell lines and four primary cell types. We identified 22,797 putative regulatory regions that directly or indirectly interact with human RPG promoters. A large proportion of these regions are only present in one cell lin ...
DOI:
10.1016/j.ygeno.2020.03.028
PubMed:
32240723
PubMed Central:
PMC7217726

4. N16 suppresses RANKL-mediated osteoclatogenesis by down-regulating RANK expression

Author:
Lin, Jia-Bi; Wu, Hao; Liu, Yu-Ling; Shaw, Pang-Chui; Li, Pei-Bo
Source:
International journal of biological macromolecules 2020 v.151 pp. 1154-1162
ISSN:
0141-8130
Subject:
cell lines,   etc   ; Pinctada martensi; macrophages; mice; monocytes; osteoclasts;     Show all 6 Subjects
Abstract:
... N16 is an active protein existing in Pinctada martensi. Our previous studies have demonstrated that N16 inhibited osteoclast differentiation in vitro. To better understand how N16 regulates osteoclast differentiation, RAW264.7 cells, a murine monocytic cell line and murine bone marrow-derived macrophages (BMMs) were adopted. Treatment of RAW264.7 cells with RANKL activated osteoclastogenesis and N ...
DOI:
10.1016/j.ijbiomac.2019.10.159

5. Citriperazines A-D produced by a marine algae-derived fungus Penicillium sp. KMM 4672

Author:
Yurchenko, Anton Nikolaevich; Berdyshev, Dmitry V.; Smetanina, Olga F.; Ivanets, Elena V.; Zhuravleva, Olesya I.; Rasin, Anton B.; Khudyakova, Yuliya V.; Popov, Roman S.; Dyshlovoy, Sergey A.; von Amsberg, Gunhild; Afiyatullov, Shamil Sh.
Source:
Natural product research 2020 v.34 no.8 pp. 1118-1123
ISSN:
1478-6427
Subject:
human cell lines,   etc   ; Penicillium; alkaloids; cytotoxicity; diketopiperazines; fungi; spectroscopy;     Show all 7 Subjects
Abstract:
... Four new diketopiperazine alkaloids, citriperazines A-D were isolated from algae-derived Penicillium sp. KMM 4672. The structures of compounds 1–4 were determined using spectroscopic methods. The absolute configurations of compounds 1 and 4 were established by comparison of calculated and experimental ECD spectra. The cytotoxicity of compounds 1–4 against several human prostate cell lines was eval ...
DOI:
10.1080/14786419.2018.1552696

6. Lessons from expanded potential of embryonic stem cells: Moving toward totipotency

Author:
Le, Rongrong; Huang, Yixin; Zhao, Anqi; Gao, Shaorong
Source:
Journal of genetics and genomics 2020 v.47 no.3 pp. 123-130
ISSN:
1673-8527
Subject:
cell lines,   etc   ; disease models; embryonic stem cells; totipotency;     Show all 4 Subjects
Abstract:
... Embryonic stem cells possess fascinating capacity of self-renewal and developmental potential, leading to significant progress in understanding the molecular basis of pluripotency, disease modeling, and reprogramming technology. Recently, 2-cell–like embryonic stem cells (ESCs) and expanded potential stem cells or extended pluripotent stem cells (EPSCs) generated from early-cleavage embryos displa ...
DOI:
10.1016/j.jgg.2020.02.003

7. Fabrication of a Smart Nanofluidic Biosensor through a Reversible Covalent Bond Strategy for High-Efficiency Bisulfite Sensing and Removal

Author:
Chen, Huan; Xu, Liying; Tuo, Wei; Chen, Xiaoya; Huang, Jinmei; Zhang, Xin; Sun, Yao
Source:
Analytical chemistry 2020 v.92 no.5 pp. 4131-4136
ISSN:
1520-6882
Subject:
cell lines,   etc   ; biochemical compounds; biosensors; bisulfites; chemical bonding; ions;     Show all 6 Subjects
Abstract:
... Bioinspired nanochannel based biosensors have been widely applied for sensing ions, small molecules, and biomolecules. However, the low selectivity and difficulty in recycle sensing still heavily hamper their widespread applications. Herein, we designed and fabricated a nanochannel based biosensor for high-efficiency bisulfite (HSO₃–) sensing and removal through forming a reversible covalent bond ...
DOI:
10.1021/acs.analchem.0c00131

8. Hi-C detects novel structural variants in HL-60 and HL-60/S4 cell lines

Author:
Jacobson, Elsie C.; Grand, Ralph S.; Perry, Jo K.; Vickers, Mark H.; Olins, Ada L.; Olins, Donald E.; O'Sullivan, Justin M.
Source:
Genomics 2020 v.112 no.1 pp. 151-162
ISSN:
0888-7543
Subject:
cell lines,   etc   ; karyotyping; loci; myeloid leukemia; neoplasm cells; sequence analysis;     Show all 6 Subjects
Abstract:
... Cancer cell lines often have large structural variants (SVs) that evolve over time. There are many reported differences in large scale SVs between HL-60 and HL-60/S4, two cell lines derived from the same acute myeloid leukemia sample. However, the stability and variability of inter- and intra-chromosomal structural variants between different sources of the same cell line is unknown. Here, we used ...
DOI:
10.1016/j.ygeno.2019.05.009

9. Structural Characteristics and Anticancer Activity In Vitro of Fucoidans from Brown Seaweeds Sargassum miyabei and S. oligocystum

Author:
Surits, V. V.; Usoltseva, R. V.; Shevchenko, N. M.; Thinh, P. D.; Ermakova, S. P.
Source:
Chemistry of natural compounds 2020 v.56 no.1 pp. 34-38
ISSN:
0009-3130
Subject:
cell lines,   etc   ; Sargassum; antineoplastic activity; cytotoxicity; fucoidan; neoplasm cells;     Show all 6 Subjects
Abstract:
... High-purity fucoidan fractions were obtained from brown seaweeds Sargassum miyabei and S. oligocystum. Both first fractions were mannnogalactofucans; second, galactofucans. Fucoidans from S. miyabei contained acetyls. The isolated polysaccharides were not cytotoxic at concentrations up to 400 μg/mL. Formation and growth of colonies of HT-29 and HCT-116 cells were weakly inhibited by the tested fuc ...
DOI:
10.1007/s10600-020-02938-y

10. Correction to: DNA copy number evolution in Drosophila cell lines

Author:
Lee, Hangnoh; McManus, C. Joel; Cho, Dong-Yeon; Eaton, Matthew; Renda, Fioranna; Somma, Maria Patrizia; Cherbas, Lucy; May, Gemma; Powell, Sara; Zhang, Dayu; Zhan, Lijun; Resch, Alissa; Andrews, Justen; Celniker, Susan E.; Cherbas, Peter; Przytycka, Teresa M.; Gatti, Maurizio; Oliver, Brian; Graveley, Brenton; MacAlpine, David
Source:
Genome biology 2019 v.20 no.1 pp. 53
ISSN:
1474-760X
Subject:
cell lines,   etc   ; DNA; Drosophila; evolution;     Show all 4 Subjects
Abstract:
... Following publication of the original article [1], the authors reported the following errors. ...
DOI:
10.1186/s13059-019-1668-5
PubMed:
30857560
PubMed Central:
PMC6410492

11. Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri

Author:
Menanteau-Ledouble, Simon; Schachner, Oskar; Lawrence, Mark L.; El-Matbouli, Mansour
Source:
Veterinary research 2020 v.51 no.1 pp. 45
ISSN:
1297-9716
Subject:
cell lines,   etc   ; Salmonidae; Yersinia ruckeri; enteric redmouth disease; fish; genes; gentamicin; pathogens; small interfering RNA;     Show all 9 Subjects
Abstract:
... Yersinia ruckeri is a facultative intracellular enterobacterium mostly known as the causative agent of enteric redmouth disease in salmonid fish. In the present study, we applied RNA inhibition to silence twenty pre-selected genes on the genome of a fish cell line (CHSE-214) followed by a gentamicin assay to quantify the effect of silencing on the cells’ susceptibility to infection and found that ...
DOI:
10.1186/s13567-020-00760-6
PubMed:
32197655
PubMed Central:
PMC7083013

12. DSN/TdT recycling digestion based cyclic amplification strategy for microRNA assay

Author:
He, Jing-Lin; Mei, Ting-Ting; Tang, Ling; Liao, Shi-Qing; Cao, Zhong
Source:
Talanta 2020 v.219 pp. 121173
ISSN:
0039-9140
Subject:
human cell lines,   etc   ; DNA; biomarkers; copper; diagnostic techniques; enzymatic reactions; fluorescence; fluorescent dyes; microRNA; neoplasms; recycling;     Show all 11 Subjects
Abstract:
... Sensitive and specific detection of microRNAs (miRNAs) is of great significance for early cancer diagnosis. Here we report a simple and sensitive fluorescence signal amplification strategy that based on DSN/TdT recycling digestion for miRNA detection. DSN initiates DNA digestion on 3′-phosphate-primer/miRNA heteroduplex which causes miRNA recycle. The digested DNA strands with 3′-OH ends enable Td ...
DOI:
10.1016/j.talanta.2020.121173

13. Biomacromolecular fluorescent nanoparticles co-assembled by bovine serum albumin and DNA segments for living cell imaging

Author:
Sun, Si; Xiao, Qian-Ru; Ye, Cui-Fang; Jiang, Yong
Source:
Colloids and surfaces 2020 v.603 pp. 125255
ISSN:
0927-7757
Subject:
human cell lines,   etc   ; DNA; bioimaging; bovine serum albumin; cost effectiveness; cytoplasm; engineering; fluorescence; nanoparticles; Michigan;     Show all 10 Subjects
Abstract:
... Fluorescent nanoparticles have been widely used in the field of biomedical engineering because they are usually biocompatible, cost-effective and homogeneously uniform. In this study, we describe a biomacromolecular fluorescent nanoparticle prepared with a facile disolvating process using bovine serum albumin, DNA segments and SYBR Green I. The obtained co-assemblies, termed DNA@BSA nanoparticles, ...
DOI:
10.1016/j.colsurfa.2020.125255

14. The fluorescent probe based on methyltetrahydroxanthylium skeleton for the detection of hydrazine

Author:
Wei, Yu-Fang; Wu, Meng-Xiang; Wei, Xue-Rui; Sun, Ru; Xu, Yu-Jie; Ge, Jian-Feng
Source:
Talanta 2020 v.218 pp. 121164
ISSN:
0039-9140
Subject:
human cell lines,   etc   ; Lewis bases; detection limit; fluorescent dyes; hydrazine; image analysis;     Show all 6 Subjects
Abstract:
... Two probes based on tetrahydroxanthylium unit (probe 1a) and methyltetrahydroxanthylium unit (probe 1b) were designed and synthesized for the detection of hydrazine. Probes 1a–b exhibited turn-on red emission signal and high selectivity toward NH₂NH₂. The response time of probe 1a to NH₂NH₂ was more than 60 min, while that of probe 1b was less than 30 min. The detection limits of probes 1a–b were ...
DOI:
10.1016/j.talanta.2020.121164

15. A microfluidic paper-based laser-induced fluorescence sensor based on duplex-specific nuclease amplification for selective and sensitive detection of miRNAs in cancer cells

Author:
Cai, Xiaoyu; Zhang, Huimin; Yu, Xiufeng; Wang, Wei
Source:
Talanta 2020 v.216 pp. 120996
ISSN:
0039-9140
Subject:
human cell lines,   etc   ; biomarkers; detection limit; fluorescence; heat; microRNA; neoplasm cells; neoplasms; paper;     Show all 9 Subjects
Abstract:
... MicroRNAs (miRNAs) are considered as the potential biomarkers for many cancers. To determine miRNAs in cancer cells is significant for realizing these diseases. In this work, a microfluidic paper-based laser-induced fluorescence sensor based on duplex-specific nuclease (DSN) amplification was developed and applied to selectively and sensitively determine miRNAs in cancer cells. An interface for la ...
DOI:
10.1016/j.talanta.2020.120996

16. Influences of calcium and magnesium ions on cellular antioxidant activity (CAA) determination

Author:
Zhou, Jingru; Gao, Guanzhen; Zhang, Suyun; Wang, Huiqin; Ke, Lijing; Zhou, Jianwu; Rao, Pingfan; Wang, Qiang; Li, Jiaxing
Source:
Food chemistry 2020 v.320 pp. 126625
ISSN:
0308-8146
Subject:
human cell lines,   etc   ; antioxidant activity; beverages; buffers; calcium; catechin; cations; dietary supplements; magnesium; quercetin;     Show all 10 Subjects
Abstract:
... The cellular antioxidant activity (CAA) assay is wildly used for quantifying antioxidant activities of foods and dietary supplements in vitro. Among various incubation and handling buffers used in different laboratories, the inconsistence in concentrations of ions, particularly calcium and magnesium, has somehow been neglected. We hired the Hank’s balanced salt solution with or without calcium and ...
DOI:
10.1016/j.foodchem.2020.126625

17. Reductive amination of α-Ketoglutarate in metabolite extracts results in glutamate overestimation

Author:
Wiese, Elizabeth K.; Hitosugi, Sadae; Buhrow, Sarah A.; Loa, Sharon T.; Sreedhar, Annapoorna; Reid, Joel M.; Gonsalves, Wilson I.; Hitosugi, Taro
Source:
Journal of chromatography 2020 v.1623 pp. 461169
ISSN:
0021-9673
Subject:
cell lines,   etc   ; alpha-ketoglutaric acid; amination; chromatography; derivatization; glutamic acid; metabolism; metabolites; metabolomics; pyridoxamine; urea;     Show all 11 Subjects
Abstract:
... Artifacts due to metabolite extraction, derivatization, and detection techniques can result in aberrant observations that are not accurate representations of actual cell metabolism. Here, we show that α-ketoglutarate (α-KG) is reductively aminated to glutamate in methanol:water metabolite extracts, which introduces an artifact into metabolomics studies. We also identify pyridoxamine and urea as am ...
DOI:
10.1016/j.chroma.2020.461169

18. MicroRNA-342 targets Cofilin 1 to suppress the growth, migration and invasion of human breast cancer cells

Author:
Liu, Cong; Xing, Hua; Luo, Xiao; Wang, Yimin
Source:
Archives of biochemistry and biophysics 2020 v.687 pp. 108385
ISSN:
0003-9861
Subject:
cell lines,   etc   ; apoptosis; bioinformatics; breast neoplasms; breasts; human diseases; luciferase; neoplasm cells; therapeutics;     Show all 9 Subjects
Abstract:
... MicroRNA-342–3p (miR-342) has been shown to act as a tumor-suppressor in different cancer types. However, the role and therapeutic implications of miR-342 via modulation of Cofilin 1 (CFL1) has not been studied in any type of cancer. Given the importance of Cofilin signalling in breast, this study was undertaken to explore the therapeutic implications of miR-342 and its target CFL1 in breast cance ...
DOI:
10.1016/j.abb.2020.108385

19. Alpinetin Inhibits Oral Squamous Cell Carcinoma Proliferation via miR-211-5p Upregulation and Notch Pathway Deactivation

Author:
Guo, Yanjun; Chen, Yong; Liu, Hongli; Yan, Wei
Source:
Nutrition and cancer 2020 v.72 no.5 pp. 757-767
ISSN:
1532-7914
Subject:
human cell lines,   etc   ; cell viability; cyclins; gene overexpression; interphase; mice; models; plasmids; squamous cell carcinoma;     Show all 9 Subjects
Abstract:
... Background: The effect of alpinetin (ALP) on miR-211-5p level and function in oral squamous cell carcinoma (OSCC) remains unclear. Materials and methods: Human OSCC cell lines (CAL-27 and TCA-8113) and a mouse xenograft model with subcutaneously injected TCA-8113 cells were used. Effect of ALP treatment on cell viability, cell cycle distributions, and p-p53, p21, c-PARP, cyclin D1, NICD, HES1, and ...
DOI:
10.1080/01635581.2019.1651878

20. In vitro gastrointestinal absorption of red wine anthocyanins – Impact of structural complexity and phase II metabolization

Author:
Han, Fuliang; Oliveira, Hélder; Brás, Natércia F.; Fernandes, Iva; Cruz, Luís; De Freitas, Victor; Mateus, Nuno
Source:
Food chemistry 2020 v.317 pp. 126398
ISSN:
0308-8146
Subject:
cell lines,   etc   ; Vitis davidii; anthocyanins; glucose; glucosides; intestinal absorption; intestines; metabolites; moieties; red wines; transporters;     Show all 11 Subjects
Abstract:
... Malvidin-3-O-glucoside, malvidin-3,5-O-diglucoside, malvidin-3-O-(6-O-coumaroyl)-glucoside-5-O-glucoside from Chinese Vitis davidii red wine were used to investigate the role of glucoside, diglucoside and coumaroylated glucoside moieties on their transport efficiency through MKN-28 gastric and Caco-2 intestinal cells. Due to the already described conversion of 3-O-glucosylated anthocyanins into 3- ...
DOI:
10.1016/j.foodchem.2020.126398