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1. Anti-Vpr activities of sesqui- and diterpenoids from the roots and rhizomes of Kaempferia candida

Author:
Prema,; Kodama, Takeshi; Nyunt, Hnin Htet Wai; Ngwe, Hla; Abe, Ikuro; Morita, Hiroyuki
Source:
Natural medicines 2021 v.75 no.3 pp. 489-498
ISSN:
1340-3443
Subject:
cell lines,   etc   ; Kaempferia; chloroform; cytotoxicity; diterpenoids; sesquiterpenoids;     Show all 6 Subjects
Abstract:
... New copaene-type and nerolidol-type sesquiterpenoids, 7-hydroxymustakone (1) and 15-hydroxynerolidol (2), and a 15-norlabdane diterpenoid, kaempcandiol (3), together with four known compounds (4–7) were isolated from the chloroform extract of Kaempferia candida roots and rhizomes. The structures of the new compounds 1–3 were elucidated based on 1D and 2D NMR and HRESIMS spectroscopic analyses. The ...
DOI:
10.1007/s11418-020-01480-z

2. Natural deep eutectic systems, an emerging class of cryoprotectant agents

Author:
Jesus, Ana Rita; Meneses, Liane; Duarte, Ana Rita C.; Paiva, Alexandre
Source:
Cryobiology 2021
ISSN:
0011-2240
Subject:
cell lines,   etc   ; cold; cryobiology; cryoprotectants; metabolites;     Show all 5 Subjects
Abstract:
... This work aimed at evaluating the potential of using natural deep eutectic systems (NADES) as cryoprotectant agents (CPAs). Several combinations between natural primary metabolites that have been identified in animals that live in extreme cold climates were prepared. All systems showed very little cytoxicity towards L929 cells at concentrations high as 1–2 M. Moreover, this cell line was highly to ...
DOI:
10.1016/j.cryobiol.2021.05.002

3. Systematic approach in macrophage polarization experiments: Maintaining integrity and reproducibility using flow cytometry and sample preparation

Author:
McCall, Jennifer R.; Sausman, Kathryn T.
Source:
Journal of immunological methods 2021 v.492 pp. 112969
ISSN:
0022-1759
Subject:
cell lines,   etc   ; flow cytometry; inflammation; macrophages;     Show all 4 Subjects
Abstract:
... Resolution of inflammation is an important physiological process following infection or injury. When inflammation fails to resolve, it can cause chronic inflammation, which exacerbates a myriad of diseases. Current anti-inflammatory treatment options are often inadequate to resolve inflammation, and as such, a key goal for drug discovery is to find natural products and novel compounds that can tar ...
DOI:
10.1016/j.jim.2021.112969

4. Precise selection of aptamers targeting PD-L1 positive small extracellular vesicles on magnetic chips

Author:
Dong, Hao-Yu; Xie, Qi-Hui; Pang, Dai-Wen; Chen, Gang; Zhang, Zhi-Ling
Source:
Chemical communications 2021 v.57 no.29 pp. 3555-3558
ISSN:
1364-548X
Subject:
cell lines,   etc   ; DNA libraries; biotinylation; magnetism; oligonucleotides;     Show all 5 Subjects
Abstract:
... A donor-cell-assisted membrane biotinylation strategy was used to modify small extracellular vesicles (sEVs) while minimizing protein damage, and allowed the sEVs to be loaded onto carriers. Biotinylated programmed death-ligand 1 (PD-L1) positive sEVs were used to select for aptamers from a DNA library. PD-L1 negative sEVs from a homologous cell line were found to remove non-specific aptamer seque ...
DOI:
10.1039/d1cc00168j

5. Non-invasive image-based cytometry for high throughput NK cell cytolysis analysis

Author:
Patel, Riya S.; Lucas, John; Timmins, Lauren M.; Mukundan, Shilpaa; Teryek, Matthew; Bhatt, Rachana; Beaulieu, Aimee; Parekkadan, Biju
Source:
Journal of immunological methods 2021 v.491 pp. 112992
ISSN:
0022-1759
Subject:
cell lines,   etc   ; coculture; cytolysis; cytotoxicity; toxicity testing;     Show all 5 Subjects
Abstract:
... Natural Killer (NK) cells are lymphocytes that are the first line of defense against malignantly transformed cells, virally infected cells and other stressed cell types. To study the cytolytic function of NK cells in vitro, a cytotoxicity assay is normally conducted against a target cancerous cell line. Current assay methods are typically performed in mixed 2D cocultures with destructive endpoints ...
DOI:
10.1016/j.jim.2021.112992

6. Microfluidic platform for synthesis and optimization of chitosan-coated magnetic nanoparticles in cisplatin delivery

Author:
Siavashy, Saeed; Soltani, M.; Ghorbani-Bidkorbeh, Fatemeh; Fallah, Newsha; Farnam, Golrokh; Mortazavi, Seyed Alireza; Shirazi, Farshad H.; Tehrani, Mohammad Hassan Houshdar; Hamedi, Mohammad Hossein
Source:
Carbohydrate polymers 2021 v.265 pp. 118027
ISSN:
0144-8617
Subject:
cell lines,   etc   ; chitosan; cisplatin; encapsulation; inhibitory concentration 50; magnetism; tripolyphosphates;     Show all 7 Subjects
Abstract:
... In this study, magnetic core/chitosan shell Nanoparticles (NPs) containing cisplatin were synthesized via cisplatin complexation with tripolyphosphate as the chitosan crosslinker using two different procedures: a conventional batch flow method and a microfluidic approach. An integrated microfluidic device composed of three stages was developed to provide precise and highly controllable mixing. The ...
DOI:
10.1016/j.carbpol.2021.118027

7. Chemical constituents and cytotoxic activity of Stachys pilifera Benth.

Author:
Khademian, Azadeh; Tabefam, Marzieh; Mazarei, Zeinab; Kanani, Mohammad Reza; Sepehri, Houri; Delphi, Ladan; Danton, Ombeline; Hamburger, Matthias; Farimani, Mahdi Moridi
Source:
South African journal of botany 2021 v.139 pp. 226-229
ISSN:
0254-6299
Subject:
cell lines,   etc   ; Stachys; breast neoplasms; cytotoxicity; flavonoids; monoacylglycerols; neoplasm cells;     Show all 7 Subjects
Abstract:
... Phytochemical investigation of the n-hexane extract of Stachys pilifera Benth. aerial parts resulted in the isolation of twelve compounds. Structures were elucidated using 1D and 2D-NMR, as well as HRMS. The purified compounds identified as one new monoglyceride (1), nine flavonoids (2–10), and two steroidal compounds. The cytotoxicity effects of purified compounds were evaluated against breast ca ...
DOI:
10.1016/j.sajb.2021.02.022

8. Caspase-3 regulates ureteric branching in mice via cell migration

Author:
Awazu, Midori; Yamaguchi, Yoshifumi; Nagata, Michio; Miura, Masayuki; Hida, Mariko
Source:
Biochemical and biophysical research communications 2021 v.559 pp. 28-34
ISSN:
0006-291X
Subject:
cell lines,   etc   ; ; caspase-3; cell movement; organ culture; research;     Show all 6 Subjects
Abstract:
... Inhibition of caspase-3 (Casp3) reduces ureteric branching in organ culture but the mechanism remains unclear. Since Casp3 has non-apoptotic functions, we examined whether Casp3 regulates ureteric branching by promoting cell migration, using a ureteric bud (UB) cell line and Casp3-deficient (Casp3−/−) mice. Also, we examined whether Casp3 plays a role in the reduced ureteric branching of metanephr ...
DOI:
10.1016/j.bbrc.2021.04.081

9. Two new phenanthrene derivatives from Pholidota chinensis

Author:
Wang, De-Lei; Lin, Fang-Xia; Tang, Min-Min; Zhou, Xue-Ming; Yi, Ji-Ling; Wang, Tian-Tian
Source:
Phytochemistry letters 2021 v.43 pp. 123-125
ISSN:
1874-3900
Subject:
human cell lines,   etc   ; cytotoxicity; neoplasm cells; phenanthrenes; plant biochemistry;     Show all 5 Subjects
Abstract:
... Two new phenanthrene derivatives, 2,3,7-trihydroxy-5H-phenanthro[4,5-bcd] pyran (1) and 2,3-dihydroxy-7-methoxy-5H-phenanthro[4,5-bcd] pyran (2) together with four known phenanthrenes derivatives (3–6) were isolated from the aerial parts of Pholidota chinensis Lindl. The structure of the new compounds 1 and 2 was determined by 1D and 2D NMR, as well as HRESIMS. The inhibitory activities of all com ...
DOI:
10.1016/j.phytol.2021.03.024

10. Evaluating the influence of progesterone concentration and time of exposure on in vitro endometrial decidualisation

Author:
Suthaporn, Sutham; Jayaprakasan, Kanna; Thornton, Jim G.; Walker, Kate F.; Castellanos, Marcos; May, Sean; Hernandez-Medrano, Juan; Maalouf, Walid E.
Source:
Molecular and cellular endocrinology 2021 v.528 pp. 111242
ISSN:
0303-7207
Subject:
cell lines,   etc   ; cell cycle; endometrium; humans; insulin; progesterone;     Show all 6 Subjects
Abstract:
... This study aimed to evaluate the influence of progesterone (concentration and time of exposure) on endometrial decidualisation using an in vitro model cell line: Human Endometrial Stromal Cells (HESCs). HESCs exposed to progesterone (1 and 10 μM) had higher percentages of decidualised cells and higher expression of the decidual marker (Insulin Like Growth Factor Binding Protein 1 (IGFBP1)) compare ...
DOI:
10.1016/j.mce.2021.111242

11. Assessing relationships between chromatin interactions and regulatory genomic activities using the self-organizing map

Author:
Kunz, Timothy; Rieber, Lila; Mahony, Shaun
Source:
Methods 2021 v.189 pp. 12-21
ISSN:
1046-2023
Subject:
cell lines,   etc   ; algorithms; chromatin; data collection; genome; genomics; humans; models;     Show all 8 Subjects
Abstract:
... Few existing methods enable the visualization of relationships between regulatory genomic activities and genome organization as captured by Hi-C experimental data. Genome-wide Hi-C datasets are often displayed using “heatmap” matrices, but it is difficult to intuit from these heatmaps which biochemical activities are compartmentalized together. High-dimensional Hi-C data vectors can alternatively ...
DOI:
10.1016/j.ymeth.2020.07.002

12. An ultrasensitive chemiluminescence strategy based on a microchip platform for telomerase detection at a single-cell level

Author:
Chen, Shengyu; Zhao, Jingjin; Shi, Bingfang; Xu, Chunhuan; Zhao, Shulin
Source:
Chemical communications 2021 v.57 no.25 pp. 3095-3098
ISSN:
1364-548X
Subject:
human cell lines,   etc   ; chemiluminescence; detection limit; microchip technology; telomerase;     Show all 5 Subjects
Abstract:
... An ultrasensitive chemiluminescence strategy based on signal amplification with a microchip platform was proposed to detect telomerase. This strategy was successfully applied to the determination of lysate samples from HL-7702, HeLa, A549 and MCF-7 cell lines with the detection limit lower than 1 HeLa cell. ...
DOI:
10.1039/d1cc00455g

13. Distinct roles of LRP5 and LRP6 in Wnt signaling regulation in the retina

Author:
Singh, Harminder D.; Ma, Jian-xing; Takahashi, Yusuke
Source:
Biochemical and biophysical research communications 2021 v.545 pp. 8-13
ISSN:
0006-291X
Subject:
cell lines,   etc   ; CRISPR-Cas systems; epithelium; humans; ligands; research; retina;     Show all 7 Subjects
Abstract:
... Dysregulation of Wnt signaling is implicated in multiple ocular disorders. The roles of Wnt co-receptors LRP5 and LRP6 in Wnt signaling regulation remain elusive, as most retinal cells express both of the co-receptors. To address this question, LRP5 and LRP6 were individually knocked-out in a human retinal pigment epithelium cell line using the CRISPR-Cas9 technology. Wnt signaling activity induce ...
DOI:
10.1016/j.bbrc.2021.01.068

14. Prenylated 4-phenylcoumarins and 4-alkylcoumarins from Kielmeyera argentea and their cytotoxic activity

Author:
Boness, Heiter V.M.; Santos, Nelson A.S.; Costa, Isis B.; Queiroz, Cintia K.L.; Marques, Edson J.; El-Bachá, Ramon S.; Cruz, Frederico G.
Source:
Fitoterapia 2021 v.149 pp. 104836
ISSN:
0367-326X
Subject:
cell lines,   etc   ; Kielmeyera; cytotoxicity; glioblastoma; humans; spectral analysis;     Show all 6 Subjects
Abstract:
... Two new prenylated 4-phenylcoumarins, named kielcoumarin A (1) and kielcoumarin B (2) together with three known compounds, mammea B/BA (3), mammea B/BA cyclo F (4) and ferruol A (5), were obtained from stems and roots of Kielmeyera argentea (Calophyllaceae). Their structures were elucidated based on spectroscopic data. Cytotoxic activity of the 4-alkylcoumarins 3–5 was evaluated in vitro against h ...
DOI:
10.1016/j.fitote.2021.104836

15. One cytotoxic steroid and other two new metabolites from the South China Sea sponge Luffariella variabilis

Author:
Luo, Xiang-Chao; Wang, Qi; Tang, Xu-Li; Li, Ping-Lin; Li, Guo-Qiang
Source:
Tetrahedron letters 2021 v.65 pp. 152762
ISSN:
0040-4039
Subject:
cell lines,   etc   ; cytotoxicity; diterpenoids; metabolites; probability analysis; South China Sea;     Show all 6 Subjects
Abstract:
... Three new compounds, including one steroid, 22,23-dihydro-24-nordankasterone A (1), one dolabellane diterpene, 6,10,18-triacetoxy-2E,7E-dolabelladien (2), and one heptadecylphenol derivative, 1-methyloxy-3-hrdroxy-4-methyl-5-heptadecylphenol (3), were isolated from the South China Sea sponge Luffariella variabilis. The planar structures were resolved by NMR and MS analyses, while the absolute conf ...
DOI:
10.1016/j.tetlet.2020.152762

16. Hybridogenesis in the Water Frogs from Western Russian Territory: Intrapopulation Variation in Genome Elimination

Author:
Miura, Ikuo; Vershinin, Vladimir; Vershinina, Svetlana; Lebedinskii, Andrei; Trofimov, Alexander; Sitnikov, Ivan; Ito, Michihiko
Source:
Genes 2021 v.12 no.2
ISSN:
2073-4425
Subject:
cell lines,   etc   ; Pelophylax; frogs; gametogenesis; hybridogenesis; hybrids; introgression; males; spermatozoa;     Show all 9 Subjects
Abstract:
... Hybridogenesis in an interspecific hybrid frog is a coupling mechanism in the gametogenic cell line that eliminates the genome of one parental species with endoduplication of the remaining genome of the other parental species. It has been intensively investigated in the edible frog Pelophylax kl. esculentus (RL), a natural hybrid between the marsh frog P. ridibundus (RR) and the pool frog P. lesso ...
DOI:
10.3390/genes12020244

17. A cell line development strategy to improve a bispecific antibody expression purity in CHO cells

Author:
Ding, Mingjian; Shen, Li; Xiao, Liang; Liu, Xiaohui; Hu, Jianzhong
Source:
Biochemical engineering journal 2021 v.166 pp. 107857
ISSN:
1369-703X
Subject:
cell lines,   etc   ; T-lymphocytes; antibodies; plasmids; polypeptides; therapeutics; transfection;     Show all 7 Subjects
Abstract:
... Among antibody-based therapies, bispecific antibodies (BsAbs) have gained momentum in preclinical and clinical investigations following the regulatory approvals of the T-cell engaging BsAb blinatunomab and the FVIII cofactor mimicking BsAb emicizumab. Production of BsAbs with high purity is one of the challenges in commercializing BsAb therapeutics. Therefore, several protein engineering approache ...
DOI:
10.1016/j.bej.2020.107857

18. Triterpenoids from the fresh pericarps of Juglans hopeiensis

Author:
Lin, Yi; Peng, Xiaogang; Chen, Juan; Zhou, Jia; Ruan, Hanli
Source:
Natural product research 2021 v.35 no.2 pp. 228-235
ISSN:
1478-6427
Subject:
cell lines,   etc   ; Juglans; cytotoxicity; neoplasm cells; pericarp; research; spectroscopy; triterpenoids;     Show all 8 Subjects
Abstract:
... Two new triterpenoids (1–2), jughopanes A (1) and jughopanes B (2), along with seventeen known ones (3–19), were isolated from the fresh pericarps of Juglans hopeiensis Hu. Their structures were settled by integrated spectroscopic techniques. All isolates were tested for their cytotoxic activities and the results showed that 7 exhibited moderate cytotoxicities against MCF7 and HT29 cancer cell lin ...
DOI:
10.1080/14786419.2019.1622111

19. Two new physalin derivatives from Physalis alkekengi L. var. franchetii (Mast.) Makino

Author:
Sun, Jian-Lu; Jiang, Yong-Jun; Cheng, Lin
Source:
Natural product research 2021 v.35 no.2 pp. 203-206
ISSN:
1478-6427
Subject:
cell lines,   etc   ; Physalis alkekengi; cytotoxicity; neoplasm cells; research;     Show all 5 Subjects
Abstract:
... Two new physalin derivatives named 7β-ethoxyl-isophysalin C (1) and 3β-ethoxyl-2,3-dihydro-4,7-didehydrophysalin B (2) were isolated from Physalis alkekengi L. var. franchetii (Mast.) Makino. Their structures were determined by extensive NMR-spectroscopic analysis and HRESIMS data. Compounds 1 and 2 were tested for their cytotoxicity against PC-3 cancer cell lines. Compound 1 showed apparent moder ...
DOI:
10.1080/14786419.2019.1619724

20. On an extended understanding of the term “hormesis” for denoting alternating directions of the organism’s response to increasing adverse exposures

Author:
Katsnelson, Boris A.; Panov, Vladimir G.; Minigalieva, Ilzira A.; Bushueva, Tatyana V.; Gurvich, Vladimir B.; Privalova, Larisa I.; Klinova, Svetlana V.; Sutunkova, Marina P.
Source:
Toxicology 2021 v.447 pp. 152629
ISSN:
0300-483X
Subject:
cell lines,   etc   ; cadmium sulfide; hormesis; in vitro studies; nanoparticles;     Show all 5 Subjects
Abstract:
... The authors propose to consider as hormesis phenomenon not only a realization of the Arndt-Schulze rule but any non-monotonic dose-response relationship for a certain outcome that is characterized by changing direction of a response between adjacent ranges of doses of an initiator of this response, the number of such ranges being two or more. This approach is illustrated with results of several in ...
DOI:
10.1016/j.tox.2020.152629