PubAg

Main content area

You searched for: Subject "cell lines" Remove constraint Subject: "cell lines"
Start Over

Search

17,220  Search Results

« Previous | 1 - 20 of 17,220 |
Number of results to display per page

Search Results

1. Correction to: DNA copy number evolution in Drosophila cell lines

Author:
Lee, Hangnoh; McManus, C. Joel; Cho, Dong-Yeon; Eaton, Matthew; Renda, Fioranna; Somma, Maria Patrizia; Cherbas, Lucy; May, Gemma; Powell, Sara; Zhang, Dayu; Zhan, Lijun; Resch, Alissa; Andrews, Justen; Celniker, Susan E.; Cherbas, Peter; Przytycka, Teresa M.; Gatti, Maurizio; Oliver, Brian; Graveley, Brenton; MacAlpine, David
Source:
Genome biology 2019 v.20 no.1 pp. 53
ISSN:
1474-760X
Subject:
cell lines,   etc   ; DNA; Drosophila; evolution;     Show all 4 Subjects
Abstract:
... Following publication of the original article [1], the authors reported the following errors. ...
DOI:
10.1186/s13059-019-1668-5

2. Synthesis and cellular uptake of carbamoylated mannose derivatives

Author:
Elferink, Hidde; Geurts, Kim; Jue, Stijn; MacCormick, Somhairle; Veeneman, Gerrit; Boltje, Thomas J.
Source:
Carbohydrate research 2019 v.481 pp. 67-71
ISSN:
0008-6215
Subject:
cell lines,   etc   ; fluorescent dyes; mannose; moieties; temperature;     Show all 5 Subjects
Abstract:
... A series of 3-carbamoyl- and 2,3-dicarbamoyl-mannose derivatives were synthesized, conjugated to a fluorescent dye (Cy5GE, AF 647 or NBD) and their cellular uptake in A549 and THP-1 cell lines was studied by FACS. In contrast to earlier studies on carbamoyl mannosides, the observed uptake was not related to carbamoyl group on the mannose residue but rather to the cyanine dye attached, a trend prev ...
DOI:
10.1016/j.carres.2019.06.008

3. Use of connectivity mapping to support read across: A deeper dive using data from 186 chemicals, 19 cell lines and 2 case studies

Author:
De Abrew, K. Nadira; Shan, Yuqing K.; Wang, Xiaohong; Krailler, Jesse M.; Kainkaryam, Raghunandan M.; Lester, Cathy C.; Settivari, Raja S.; LeBaron, Matthew J.; Naciff, Jorge M.; Daston, George P.
Source:
Toxicology 2019 v.423 pp. 84-94
ISSN:
0300-483X
Subject:
cell lines,   etc   ; case studies; chemical structure; toxicology;     Show all 4 Subjects
Abstract:
... We previously demonstrated that the Connectivity Map (CMap) (Lamb et al., 2006) concept can be successfully applied to a predictive toxicology paradigm to generate meaningful MoA-based connections between chemicals (De Abrew et al., 2016). Here we expand both the chemical and biological (cell lines) domain for the method and demonstrate two applications, both in the area of read across. In the fir ...
DOI:
10.1016/j.tox.2019.05.008

4. Nanoamplicon Comparator for Live-Cell MicroRNA Imaging

Author:
Huo, Min; Li, Siqiao; Zhang, Peiwen; Feng, Yimei; Liu, Yiran; Wu, Na; Ju, Huangxian; Ding, Lin
Source:
Analytical chemistry 2019 v.91 no.5 pp. 3374-3381
ISSN:
1520-6882
Subject:
cell lines,   etc   ; image analysis; microRNA; nanoparticles;     Show all 4 Subjects
Abstract:
... As an investigative tool, live-cell imaging requires superior probe design to guarantee imaging quality and data validity. The ability to simultaneously address the robustness, sensitivity, and consistency issues in a single-assay system is highly desired, but it remains a largely unsolved challenge. We describe herein a probe-design strategy called a nanoamplicon comparator (NAC) and demonstrate ...
DOI:
10.1021/acs.analchem.8b04661

5. Correction: Stability of gum Arabic-gold nanoparticles in physiological simulated pHs and their selective effect on cell lines

Author:
Ribeiro de Barros, Heloise; Cardoso, Mateus Borba; Camargo de Oliveira, Carolina; Cavichiolo Franco, Célia Regina; de Lima Bellan, Daniel; Vidotti, Marcio; Riegel-Vidotti, Izabel C.
Source:
RSC advances 2018 v.8 no.71 pp. 40596
ISSN:
2046-2069
Subject:
cell lines,   etc   ; nanoparticles; pH;     Show all 3 Subjects
Abstract:
... Correction for ‘Stability of gum Arabic-gold nanoparticles in physiological simulated pHs and their selective effect on cell lines’ by Heloise Ribeiro de Barros et al., RSC Adv., 2016, 6, 9411–9420. ...
DOI:
10.1039/c8ra90090f

6. Correction to: Sequence and structural variation in the genome of the Biomphalaria glabrata embryonic (Bge) cell line

Author:
Wheeler, Nicolas J.; Dinguirard, Nathalie; Marquez, Joshua; Gonzalez, Adrian; Zamanian, Mostafa; Yoshino, Timothy P.; Castillo, Maria G.
Source:
Parasites & vectors 2018 v.11 no.1 pp. 566
ISSN:
1756-3305
Subject:
cell lines,   etc   ; Biomphalaria glabrata; genome;     Show all 3 Subjects
Abstract:
... Following publication of the original article [], the authors reported an error in figure 1: ...
DOI:
10.1186/s13071-018-3135-7

7. Comparative analysis of sequencing technologies for single-cell transcriptomics

Author:
Natarajan, Kedar Nath; Miao, Zhichao; Jiang, Miaomiao; Huang, Xiaoyun; Zhou, Hongpo; Xie, Jiarui; Wang, Chunqing; Qin, Shishang; Zhao, Zhikun; Wu, Liang; Yang, Naibo; Li, Bo; Hou, Yong; Liu, Shiping; Teichmann, Sarah A.
Source:
Genome biology 2019 v.20 no.1 pp. 70
ISSN:
1474-760X
Subject:
cell lines,   etc   ; RNA; complementary DNA; sequence analysis; transcriptomics;     Show all 5 Subjects
Abstract:
... Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq platform for scRNA-seq. We generate a resource of 468 single cells and 1297 matched single cDNA samples, performing SMARTer and Smart-seq2 protocols on two cell lines with RNA spike-ins. We sequence these libraries on b ...
DOI:
10.1186/s13059-019-1676-5

8. Modeling double strand break susceptibility to interrogate structural variation in cancer

Author:
Ballinger, Tracy J.; Bouwman, Britta A. M.; Mirzazadeh, Reza; Garnerone, Silvano; Crosetto, Nicola; Semple, Colin A.
Source:
Genome biology 2019 v.20 no.1 pp. 28
ISSN:
1474-760X
Subject:
cell lines,   etc   ; DNA; DNA damage; chromatin; genes; models; mutation; neoplasms;     Show all 8 Subjects
Abstract:
... BACKGROUND: Structural variants (SVs) are known to play important roles in a variety of cancers, but their origins and functional consequences are still poorly understood. Many SVs are thought to emerge from errors in the repair processes following DNA double strand breaks (DSBs). RESULTS: We used experimentally quantified DSB frequencies in cell lines with matched chromatin and sequence features ...
DOI:
10.1186/s13059-019-1635-1

9. Cell-line characterization by infrared-induced pyroelectric effect

Author:
Pullano, Salvatore A.; Greco, Marta; Corigliano, Domenica M.; Foti, Daniela. P.; Brunetti, A.; Fiorillo, Antonino S.
Source:
Biosensors & bioelectronics 2019 v.140 pp. 111338
ISSN:
0956-5663
Subject:
cell lines,   etc   ; absorption; biosensors; cell viability; models; thermal conductivity;     Show all 6 Subjects
Abstract:
... Evaluation of cellular thermodynamics has recently received a high interest because of its implication in many mechanisms related with function, structure and health of cells. Recent literature reported significant efforts to provide affordable intracellular thermal components of absorption, such as thermal conductivity, to overcome the lack of experimental data.Herein, we provide lines of evidenc ...
DOI:
10.1016/j.bios.2019.111338

10. Vibralactones U–W, three vibralactone derivatives from cultures of the basidiomycete Boreostereum vibrans

Author:
Duan, Kai-Ting; Li, Zheng-Hui; Wang, Wen-Xuan; Chen, He-Ping; Sun, Huan; Huang, Rong; Feng, Tao; Liu, Ji-Kai
Source:
Journal of Asian natural products research 2019 v.21 no.7 pp. 603-609
ISSN:
1477-2213
Subject:
human cell lines,   etc   ; Boreostereum; humans; neoplasm cells; spectroscopy;     Show all 5 Subjects
Abstract:
... Three new vibralactone derivatives, namely vibralactones U–W (1–3), together with vibralactone (4), have been isolated from cultures of the basidiomycete Boreostereum vibrans. Their structures were determined on the basis of spectroscopic methods and literature data. All compounds showed no activities to five human cancer cell lines. ...
DOI:
10.1080/10286020.2018.1461844

11. Model-based optimization of temperature and pH shift to increase volumetric productivity of a Chinese hamster ovary fed-batch process

Author:
Paul, Katrin; Rajamanickam, Vignesh; Herwig, Christoph
Source:
Journal of bioscience and bioengineering 2019
ISSN:
1389-1723
Subject:
cell lines,   etc   ; biochemical pathways; biopharmaceuticals; markets; mechanistic models; pH; temperature;     Show all 7 Subjects
Abstract:
... Industrial production processes, which utilize mammalian cells for the production of therapeutic proteins are routinely designed to include temperature and pH shifts. The process conditions are shifted away from growth promoting conditions towards a state of decreased metabolic activity. This results in the extension of the cultivation duration and therefore in a higher final product concentration ...
DOI:
10.1016/j.jbiosc.2019.06.004

12. Cell‐permeable organic fluorescent probes for live‐cell super‐resolution imaging of actin filaments

Author:
Han, Yubing; Li, Meihua; Zhang, Meng; Huang, Xiaoshuai; Chen, Liangyi; Hao, Xiang; Kuang, Cuifang; Liu, Xu; Zhang, Yu‐Hui
Source:
Journal of chemical technology and biotechnology 2019 v.94 no.6 pp. 2040-2046
ISSN:
0268-2575
Subject:
cell lines,   etc   ; actin; fluorescent dyes; image analysis; microfilaments; microscopy; peptides;     Show all 7 Subjects
Abstract:
... BACKGROUND: The recently developed super‐resolution microscopy has been applied to characterize the nanometer‐scale structure of actin filaments in live cells, owing to its live‐cell imaging ability at sub‐diffraction resolution. However, there are still some limitations to existing live‐cell labeling methods for super‐resolution imaging of F‐actin. RESULTS: A series of cell‐permeable fluorescent ...
DOI:
10.1002/jctb.5990

13. Heterogeneity Studies of Mammalian Cells for Bioproduction: From Tools to Application

Author:
Schmitz, Julian; Noll, Thomas; Grünberger, Alexander
Source:
Trends in biotechnology 2019 v.37 no.6 pp. 645-660
ISSN:
0167-7799
Subject:
cell lines,   etc   ; bioprocessing; droplets; fermentation; flow cytometry; mammals; microfluidic technology;     Show all 7 Subjects
Abstract:
... Quantified process parameters in pharmaceutical production and in mammalian cell fermentation process development only represent culture average values and do not portray the heterogeneity and the individual behaviour of single cells. The first studies using well-established techniques like flow cytometry or novel lab-on-a-chip systems, such as droplet microfluidics or microfluidic single-cell cul ...
DOI:
10.1016/j.tibtech.2018.11.007

14. SHP-1 inhibits renal ischemia reperfusion injury via dephosphorylating ASK1 and suppressing apoptosis

Author:
Tian, Hongzhe; Tan, Rumeng; Ye, Bogen; Yan, Sijia; Sui, Mingxing; Zhao, Wenyu; Zhang, Lei; Zhu, Youhua; Zeng, Li
Source:
Biochemical and biophysical research communications 2019 v.513 no.2 pp. 360-367
ISSN:
0006-291X
Subject:
cell lines,   etc   ; apoptosis; enzymes; epithelium; ischemia; protective effect; reperfusion injury;     Show all 7 Subjects
Abstract:
... Apoptosis of tubular epithelium cells (TECs) plays critical roles in renal ischemia reperfusion (I/R) injury, but the molecular regulatory mechanisms of apoptosis still require further investigation. Recently, phosphatase family members have been suggested to regulate multiple aspects of the injury and regeneration response. However, the roles of SHP-1, an important protein-tyrosine phosphatase, i ...
DOI:
10.1016/j.bbrc.2019.03.187

15. Hi-C detects novel structural variants in HL-60 and HL-60/S4 cell lines

Author:
Jacobson, Elsie C.; Grand, Ralph S.; Perry, Jo K.; Vickers, Mark H.; Olins, Ada L.; Olins, Donald E.; O'Sullivan, Justin M.
Source:
Genomics 2019
ISSN:
0888-7543
Subject:
cell lines,   etc   ; karyotyping; loci; myeloid leukemia; neoplasm cells; sequence analysis;     Show all 6 Subjects
Abstract:
... Cancer cell lines often have large structural variants (SVs) that evolve over time. There are many reported differences in large scale SVs between HL-60 and HL-60/S4, two cell lines derived from the same acute myeloid leukemia sample. However, the stability and variability of inter- and intra-chromosomal structural variants between different sources of the same cell line is unknown. Here, we used ...
DOI:
10.1016/j.ygeno.2019.05.009

16. Gaussian FRET two-hybrid assays for determining the stoichiometry of hetero-oligomeric complexes in single living cells

Author:
Qu, Wenfeng; Du, Mengyan; Yang, Fangfang; Mai, Zihao; Zhang, Chenshuang; Lin, Fangrui; Ma, Yunyun; Chen, Tongsheng
Source:
Biochemical and biophysical research communications 2019 v.512 no.3 pp. 492-497
ISSN:
0006-291X
Subject:
human cell lines,   etc   ; energy transfer; fluorescence; mitochondria; plasmids; stoichiometry;     Show all 6 Subjects
Abstract:
... Here we integrate multiple Gaussian-functions analysis into fluorescence resonance energy transfer (FRET) two-hybrid assays (Gaussian FRET two-hybrid assay) to determine the stoichiometric ratios of intracellular hetero-oligomers in single living cells. This method adopts in multiple Gaussian-functions to fit the E-count histograms of both donor- and acceptor-centric FRET efficiency (ED and EA) im ...
DOI:
10.1016/j.bbrc.2019.03.089

17. A new norisoprenoid from the leaves of Ficus pumila

Author:
Bai, Meng; Cai, Yue; Wu, Shou-Yuan; Song, Xiao-Ping; Chen, Guang-Ying; Zheng, Cai-Juan; Han, Chang-Ri
Source:
Natural product research 2019 v.33 no.9 pp. 1292-1297
ISSN:
1478-6427
Subject:
cell lines,   etc   ; Ficus pumila; cytotoxicity; ethanol; leaves; spectral analysis; China;     Show all 7 Subjects
Abstract:
... One new norisoprenoid 3,9-dihydroxy dihydro actinidiolide (1), together with eleven known compounds (2–12), were isolated from ethanol extract of the leaves of Ficus pumila collected in Hainan Province, China. Their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. Compounds 1, 2, 4, 5, 8 and 10–12 were isolated from the F. pumila for the first time. All comp ...
DOI:
10.1080/14786419.2018.1471077

18. Comparative analysis of replicative properties of phylogenetically divergent, Argentinean BoHV-4 strains in cell lines from different origins

Author:
Morán, Pedro; Pérez, Sandra; Odeón, Anselmo; Verna, Andrea
Source:
Comparative immunology, microbiology, and infectious diseases 2019 v.63 pp. 97-103
ISSN:
0147-9571
Subject:
cell lines,   etc   ; Herpesviridae; cows; cytopathogenicity; genotype; growth models; phylogeny; Argentina;     Show all 8 Subjects
Abstract:
... Bovine gammaherpesvirus 4 (BoHV4) is a member of the family Herpesviridae. In Argentina, BoHV4 was isolated and characterized in 2007 from samples of aborted cows. Argentinean isolates are highly divergent and are classified as: Genotype 1(Movar-like), Genotype 2 (DN599-like) and Genotype 3 (a novel group). The aim of this study was to comparatively evaluate the biological characteristics of six A ...
DOI:
10.1016/j.cimid.2019.01.014

19. Cryopreservation of cell line Paesun by a rapid cooling

Author:
Song, Jong Kui; Hui, Yu Lan
Source:
Cryobiology 2019 v.87 pp. 115-116
ISSN:
0011-2240
Subject:
cell lines,   etc   ; cooling; cryopreservation; liquids; nitrogen; staining; thawing; vapors; viruses;     Show all 9 Subjects
Abstract:
... The purpose of the present study was to clarify the possibility of a rapid cryopreservation for cell line Paesun by cooling in the range of 30–40 °C/min to vapor phase of −120 ∼-140 °C before immersion into liquid phase of liquid nitrogen using 10% Me2SO. After thawing, these cells were examined with assaying viability by trypan blue exclusion staining and survival by cloning in monolayer; the per ...
DOI:
10.1016/j.cryobiol.2019.01.017

20. Laser interference‐based technique for dynamic measurement of single cell deformation manipulated by optical tweezers

Author:
Liu, Jiaqi; Zhang, Fan; Zhu, Lianqing; Qu, Xinghua; Chu, Daping
Source:
Electrophoresis 2019 v.40 no.8 pp. 1160-1165
ISSN:
0173-0835
Subject:
human cell lines,   etc   ; cameras; deformation; electrophoresis; erythrocytes; optical traps; semiconductors;     Show all 7 Subjects
Abstract:
... A laser interference‐based method was proposed to measure the deformation response of cell manipulated by optical tweezers. This method was implemented experimentally by integrating a laser illuminating system and optical tweezers with an inverted microscope. Interference fringes generated by the transmitted and reflected lights were recorded by a complementary metal oxide semiconductor camera. Fr ...
DOI:
10.1002/elps.201800336