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1. Biflavonoids from the twigs and leaves of Cephalotaxus oliveri Mast. and their α-glucosidase inhibitory activity

Author:
Min Song; Tong Xiao; Qiu-Shuang Wu; Hang Fai Kwok; Ging Chan; Li-Gen Lin; Xiao-Qi Zhang; Wen-Cai Ye; Sheng-Jiang He; Qing-Wen Zhang
Source:
Natural product research 2022 v.36 no.12 pp. 3085-3094
ISSN:
1478-6427
Subject:
cell lines,   etc   ; Cephalotaxus; cytotoxicity; flavonoids; research;     Show all 5 Subjects
Abstract:
... Three new biflavonoids, umcephabiovins C − E (1 − 3), along with fourteen known compounds were isolated from the twigs and leaves of Cephalotaxus oliveri. Their structures and configurations were elucidated by UV, IR, NMR, ECD, and HR-ESI-MS spectra. Compounds 1 − 3 exhibited significant α-glucosidase inhibitory activity with IC₅₀ values of 7.05 ± 2.66, 24.45 ± 4.73, and 1.84 ± 1.14 μM, respective ...
DOI:
10.1080/14786419.2021.1958328

2. DeepRepeat: direct quantification of short tandem repeats on signal data from nanopore sequencing

Author:
Li Fang; Qian Liu; Alex Mas Monteys; Pedro Gonzalez-Alegre; Beverly L. Davidson; Kai Wang
Source:
Genome biology 2022 v.23 no.1 pp. 108
ISSN:
1474-760X
Subject:
cell lines,   etc   ; data collection; genome; nanopores; telomeres;     Show all 5 Subjects
Abstract:
... Despite recent improvements in basecalling accuracy, nanopore sequencing still has higher error rates on short-tandem repeats (STRs). Instead of using basecalled reads, we developed DeepRepeat which converts ionic current signals into red-green-blue channels, thus transforming the repeat detection problem into an image recognition problem. DeepRepeat identifies and accurately quantifies telomeric ...
DOI:
10.1186/s13059-022-02670-6

3. Detection of cell membrane proteins using ion-sensitive field effect transistors combined with chemical signal amplification

Author:
Miyuki Tabata; Chattarika Khamhanglit; Sayo Kotaki; Yuji Miyahara
Source:
Chemical communications 2022 v.58 no.53 pp. 7368-7371
ISSN:
1364-548X
Subject:
cell lines,   etc   ; cell membranes; membrane proteins; urea;     Show all 4 Subjects
Abstract:
... The capture and detection of cells expressing a breast-cancer related membrane protein, namely a BT474 cell line expressing HER2, is demonstrated using ion-sensitive field effect transistors (ISFETs). BT474 cells were exposed to anti-HER2 antibodies and urease-conjugated secondary antibodies to induce chemical signal amplification by adding urea. ...
DOI:
10.1039/d2cc02159e

4. Linc-ROR has a Potential ceRNA Activity for OCT4A by Sequestering miR-335-5p in the HEK293T Cell Line

Author:
Elham Taheri Bajgan; Akram Gholipour; Mohammadali Faghihi; Seyed Javad Mowla; Mahshid Malakootian
Source:
Biochemical genetics 2022 v.60 no.3 pp. 1007-1024
ISSN:
0006-2928
Subject:
cell lines,   etc   ; bioinformatics; luciferase; microRNA; stem cells;     Show all 5 Subjects
Abstract:
... Linc-ROR has a regulatory role in reprogramming, and the core stem cell transcription factors, OCT4, SOX2, and NANOG, regulate its expression. MicroRNAs (miRNAs) are also a critical constituent of pivotal posttranscriptional regulatory pathways. One of such interactions is a competing endogenous RNA interaction that connects small and long non-coding RNAs with coding transcripts. Here, we aimed to ...
DOI:
10.1007/s10528-021-10140-0

5. Boosting effect of testosterone on GDNF expression in Sertoli cell line (TM4); comparison between TM3 cells-produced and exogenous testosterone

Author:
Himasadat Zaker; Mazdak Razi; Alireza Mahmoudian; Farhad Soltanalinejad
Source:
Gene 2022 v.812 pp. 146112
ISSN:
0378-1119
Subject:
cell lines,   etc   ; Sertoli cells; coculture; genes; spermatogenesis; testosterone;     Show all 6 Subjects
Abstract:
... The Glial cell-derived neurotrophic factor (Gdnf) and testosterone induce the spermatogonial stem cells (SSCs) self-renewal and spermatogenesis, respectively. In present study the stimulating role of testosterone on Sertoli cells to produce Gdnf, and the possible effect of Gdnf on Gfrα1 and c-RET expressions were investigated. The TM4 cells (line Sertoli cells) were co-cultured with [0.1, 0.2 and ...
DOI:
10.1016/j.gene.2021.146112

6. Phenolic glycosides from Oroxylum indicum

Author:
Le Thi Vien; Tran Thi Hong Hanh; Tran Hong Quang; Nguyen The Cuong; Nguyen Xuan Cuong; Hyuncheol Oh; Nguyen Van Sinh; Nguyen Hoai Nam; Chau Van Minh
Source:
Natural product research 2022 v.36 no.9 pp. 2336-2340
ISSN:
1478-6427
Subject:
cell lines,   etc   ; Oroxylum indicum; bark; glycosides; neuroglia; research;     Show all 6 Subjects
Abstract:
... Two new phenolic glycosides, oroxylumosides A (1) and B (2), along with four known compounds darendoside A (3), leucosceptoside A (4), acteoside (5) and decaffeoylacteoside (6) were isolated from the stem bark of Oroxylum indicum. Their structures were elucidated by extensive analysis of the 1 D and 2 D NMR as well as HR-ESI-QTOF-MS. In addition, compounds 1 − 4 exhibited inhibitory effects on NO ...
DOI:
10.1080/14786419.2020.1834548

7. Total mRNA and primary human myoblasts’ in vitro cell cycle progression distinguishes between clones

Author:
Kirankumar B. Gudagudi; Niccolò Passerin d’Entrèves; Tracey Ollewagen; Kathryn H. Myburgh
Source:
Biochimie 2022 v.196 pp. 161-170
ISSN:
0300-9084
Subject:
cell lines,   etc   ; humans; interphase; myoblasts; transcription (genetics); transcriptome;     Show all 6 Subjects
Abstract:
... Satellite cells are generally quiescent in vivo. Once activated, progression through the cell cycle begins. Immortalised myoblasts from a single cell line are fairly homogenous in culture, but primary human myoblasts (PHMs) demonstrate heterogeneity. This phenomenon is poorly understood however may impact on PHM expansion. This study aimed to evaluate cell cycle transition from growth to synthesis ...
DOI:
10.1016/j.biochi.2022.01.010

8. Development of a CHO cell line for stable production of recombinant antibodies against human MMP9

Author:
Jina Ryu; Eun-Jung Kim; Joo-Kyung Kim; Tai Hyun Park; Byung-Gee Kim; Hee-Jin Jeong
Source:
BMC biotechnology 2022 v.22 no.1 pp. 8
ISSN:
1472-6750
Subject:
cell lines,   etc   ; Cricetulus griseus; biomarkers; biotechnology; gelatinase B; humans;     Show all 6 Subjects
Abstract:
... BACKGROUND: Human matrix metalloproteinase 9 (hMMP9) is a biomarker in several diseases, including cancer, and the need for developing detectors and inhibitors of hMMP9 is increasing. As an antibody against hMMP9 can be selectively bound to hMMP9, the use of anti-MMP9 antibody presents new possibilities to address hMMP9-related diseases. In this study, we aimed to establish a stable Chinese hamste ...
DOI:
10.1186/s12896-022-00738-6

9. Isolation of Nannocystis species from Iran and exploring their natural products

Author:
Azam Moradi; Mohammad Yaghoubi-Avini; Joachim Wink
Source:
Archives of microbiology 2022 v.204 no.2 pp. 123
ISSN:
0302-8933
Subject:
cell lines,   etc   ; cytotoxicity; fibroblasts; new species; secondary metabolites; Iran;     Show all 6 Subjects
Abstract:
... Several different techniques were employed for the isolation of Nannocystis from various sources. A polyphasic approach was used for identification. Twelve strains of N. pusilla, N. exedens, and N. konarekensis with distinctive distribution between climates were identified. The bioactivity was examined against a panel of eight bacteria, two yeasts, and one fungus; cytotoxicity was tested on the L9 ...
DOI:
10.1007/s00203-021-02738-0

10. Phenylpropanoids from Brachybotrys paridiformis Maxim. ex Oliv. and their anti-HBV activities

Author:
Si-Tong Wu; Yi-Xiao Wang; Bai-Hong Yu; Chun-Liu Ma; He-Qin Qiu; Guang-Shu Wang
Source:
Phytochemistry 2022 v.197 pp. 113114
ISSN:
0031-9422
Subject:
cell lines,   etc   ; anhydrides; phenylpropanoids; plant biochemistry; spectral analysis; viruses;     Show all 6 Subjects
Abstract:
... Using chemical and spectroscopic data, this study on Brachybotrys paridiformis Maxim. ex Oliv. identified four undescribed phenylpropanoids, brachin A–C and brachoside A, together with nine other known compounds. The isolated compounds were tested for anti-hepatitis B virus activities in the HepG2.2.15 cell line. Among them, caffeic anhydride showed the most potent activity. ...
DOI:
10.1016/j.phytochem.2022.113114

11. Synthesis of Marine Cyclopeptide Galaxamide Analogues as Potential Anticancer Agents

Author:
Daichun Li; Xiaojian Liao; Shenghui Zhong; Bingxin Zhao; Shihai Xu
Source:
Marine drugs 2022 v.20 no.3 pp. -
ISSN:
1660-3397
Subject:
human cell lines,   etc   ; apoptosis; cytotoxicity; flow cytometry; neoplasm cells;     Show all 5 Subjects
Abstract:
... In this paper, eight new galaxamide analogues (Z-1~Z-8) were synthesized and evaluated for their cytotoxic activities against five cancer cell lines, MCF-7, MD-MBA-231, HepG2, Hela, and A549, using MTT assays. The modified analogue Z-6 displayed broad spectrum cytotoxic activity toward each tested cell line with IC₅₀ values of 1.65 ± 0.30 (MCF-7), 2.91 ± 0.17 (HepG2), 4.59 ± 0.27 (MD-MBA-231), 5.6 ...
DOI:
10.3390/md20030158

12. C18-Functionalized Amine-Bridged Hybrid Monoliths for Mass Spectrometry-Friendly Peptide Separation and Highly Sensitive Proteomic Analysis

Author:
Yu Liang; Chao Wang; Zhen Liang; Lihua Zhang; Yukui Zhang
Source:
Analytical chemistry 2022 v.94 no.16 pp. 6084-6088
ISSN:
1520-6882
Subject:
human cell lines,   etc   ; adsorption; analytical chemistry; mass spectrometry; peptides; proteomics;     Show all 6 Subjects
Abstract:
... For proteomic analysis based on mass spectrometry (MS), high-performance peptide separation under MS-friendly conditions is of importance. To this end, a novel kind of amine-bridged hybrid monolith was developed by the sol–gel reaction of bis[3-(trimethoxysilyl)propyl]amine and allyltrimethoxysilane, followed by “thiol–ene” click functionalization of C18 groups. With the secondary amino groups bri ...
DOI:
10.1021/acs.analchem.1c04405

13. Cytotoxic activity in basal and tumoral cell lines of the C0K3N3 protein from the snake venom Crotalus durissus collilineatus, variety crotamine negative

Author:
Sayonara Ay More; Larissa Cleres Moreira; Marta Regina Magalhães; Marize Campos Valadares; Luiz Carlos da Cunha
Source:
Toxicon 2022 v.210 pp. 155-157
ISSN:
0041-0101
Subject:
cell lines,   etc   ; Crotalus durissus; cytotoxicity; neoplasm cells; snake venoms; therapeutics;     Show all 6 Subjects
Abstract:
... Snake venoms are natural sources of bioactive substances with therapeutic potential. In this work, we evaluated the cytotoxicity of the Crotalus durissus collilineatus, negative crotamine variety and the isolated fraction C0K3N3 in BALB C/3T3 and K562 cell lines. The results indicate that the C0K3N3 protein is more cytotoxic against the K562 tumor cell line than in the 3T3 baseline. ...
DOI:
10.1016/j.toxicon.2022.02.021

14. Immunoassay for trypsinogen-4

Author:
Hannu Koistinen; Riitta Koistinen; Kristina Hotakainen; Anna Lempiäinen; Kalle Jokelainen; Martti Färkkilä; Ulf-Håkan Stenman
Source:
Analytical biochemistry 2022 v.648 pp. 114681
ISSN:
0003-2697
Subject:
cell lines,   etc   ; astrocytes; bile; genes; immunoassays; isozymes; signal peptide; trypsin;     Show all 8 Subjects
Abstract:
... Trypsin has been identified as a pancreatic protease comprising three isoenzymes, trypsin-1, -2, and -3. However, the gene for trypsinogen-3, PRSS3, also gives rise to additional variants, trypsinogen-4A and B, which differ from trypsinogen-3 only with respect to the leader-peptide part, and when activated are identical to trypsin-3. The unique overlapping leader peptides of trypsinogen-4A and B a ...
DOI:
10.1016/j.ab.2022.114681

15. Porcine and Chicken Intestinal Epithelial Cell Models for Screening Phytogenic Feed Additives—Chances and Limitations in Use as Alternatives to Feeding Trials

Author:
Hannah Marks; Łukasz Grześkowiak; Beatriz Martinez-Vallespin; Heiko Dietz; Jürgen Zentek
Source:
Microorganisms 2022 v.10 no.3 pp. -
ISSN:
2076-2607
Subject:
cell lines,   etc   ; chickens; coculture; epithelial cells; farms; models; organoids; swine;     Show all 8 Subjects
Abstract:
... Numerous bioactive plant additives have shown various positive effects in pigs and chickens. The demand for feed additives of natural origin has increased rapidly in recent years to support the health of farm animals and thus minimize the need for antibiotics and other drugs. Although only in vivo experiments can fully represent their effect on the organism, the establishment of reliable in vitro ...
DOI:
10.3390/microorganisms10030629

16. Prevalidation of the cAMP-PTx reporter assay for quantitative assessment of pertussis toxin activity

Author:
L. de Brouwer; D. David; M. Espitia Ballestas; A. Sloots; M.E. Hoonakker
Source:
Vaccine 2022 pp. -
ISSN:
0264-410X
Subject:
cell lines,   etc   ; forskolin; histamine; mice; pertussis toxin; toxicity; vaccines; Europe;     Show all 8 Subjects
Abstract:
... Testing for inactivation of pertussis toxin and reversion to toxicity in aP vaccines has historically relied on the murine histamine sensitization test, that lacks mechanistic understanding, suffers from standardization problems and is associated with severe animal suffering. Though the regulatory requirements for in vivo testing of acellular pertussis (aP) vaccine products have been waived in Eur ...
DOI:
10.1016/j.vaccine.2022.04.068

17. Secretory phospholipase sPLA2-IIAloss impairs tumorigenic and metastatic potential in breast cancer cells

Author:
Darshan Mehta; Sayoni Roy; Priyanka Joshi; Mitali Parab; Sanjeev K. Waghmare
Source:
Biochemical and biophysical research communications 2022 v.597 pp. 102-108
ISSN:
0006-291X
Subject:
human cell lines,   etc   ; breast neoplasms; cell proliferation; metastasis; phospholipases; research;     Show all 6 Subjects
Abstract:
... Breast cancer stem cells (BCSCs) are slow cycling cells that escape the traditional chemo-radio-therapy, thereby contributing in resistance and recurrence. Although several markers have been identified, it is still challenging to develop strategies targeting them. In this study, we have isolated BCSCs from MCF-7 cell line using markers CD44⁺/CD24⁻/ˡᵒʷ, which showed higher percentage of mammosphere ...
DOI:
10.1016/j.bbrc.2022.01.079

18. (±)-Involucrasins A and B, two pairs of flavanone enantiomers from Shuteria involucrata and their inhibitory effects on the proliferation of various cancer cell lines

Author:
Lu Liu; Xiao-Hong Li; Xiao-Xia Ma; Wen-Hong Tan; Wen-Zhe Ma; Yun-Fu Shen; Afsar Khan; Zhi-Hong Zhou; Zhu-Ya Yang
Source:
Journal of Asian natural products research 2022 v.24 no.7 pp. 641-647
ISSN:
1477-2213
Subject:
human cell lines,   etc   ; Shuteria; enantiomers; flavanones; neoplasm cells; research; spectral analysis;     Show all 7 Subjects
Abstract:
... (±)-Involucrasins A (1) and B (2), two pairs of flavanone enantiomers were isolated from Shuteria involucrata. Structurally, both 1 and 2 are rare representatives of 5-dehydroxy/5-demethoxy 2’,3’,4’-trisubstituted flavanones. Their structures were elucidated on the basis of comprehensive spectroscopic data analysis and comparison with the literature data. Involucrasin B (2) exhibited moderate anti ...
DOI:
10.1080/10286020.2021.1952187

19. Adhesion and aggregation properties of Lactobacillaceae strains as protection ways against enteropathogenic bacteria

Author:
Anna Zawistowska-Rojek; Anita Kośmider; Karolina Stępień; Stefan Tyski
Source:
Archives of microbiology 2022 v.204 no.5 pp. 285
ISSN:
0302-8933
Subject:
human cell lines,   etc   ; Escherichia coli; adhesion; digestive tract; enteropathogens; intestines; probiotics;     Show all 7 Subjects
Abstract:
... The adhesion and aggregation are characteristic attributes of probiotic strains belonging to Lactobacillaceae genus. Due to these properties the host organisms can avoid colonisation of the intestinal tract by enteropathogenic bacteria. The presented research includes a comparison of the properties of various strains belonging to different Lactobacillaceae species and isolated from different sourc ...
DOI:
10.1007/s00203-022-02889-8

20. Deep oncopanel sequencing reveals within block position-dependent quality degradation in FFPE processed samples

Author:
Yifan Zhang; Thomas M. Blomquist; Rebecca Kusko; Daniel Stetson; Zhihong Zhang; Lihui Yin; Robert Sebra; Binsheng Gong; Jennifer S. Lococo; Vinay K. Mittal; Natalia Novoradovskaya; Ji-Youn Yeo; Nicole Dominiak; Jennifer Hipp; Amelia Raymond; Fujun Qiu; Hanane Arib; Melissa L. Smith; Jay E. Brock; Daniel H. Farkas; Daniel J. Craig; Erin L. Crawford; Dan Li; Tom Morrison; Nikola Tom; Wenzhong Xiao; Mary Yang; Christopher E. Mason; Todd A. Richmond; Wendell Jones; Donald J. Johann Jr; Leming Shi; Weida Tong; James C. Willey; Joshua Xu
Source:
Genome biology 2022 v.23 no.1 pp. 141
ISSN:
1474-760X
Subject:
cell lines,   etc   ; DNA; diploidy; formalin; genome; genomics; histology; mutation; patients; therapeutics;     Show all 10 Subjects
Abstract:
... BACKGROUND: Clinical laboratories routinely use formalin-fixed paraffin-embedded (FFPE) tissue or cell block cytology samples in oncology panel sequencing to identify mutations that can predict patient response to targeted therapy. To understand the technical error due to FFPE processing, a robustly characterized diploid cell line was used to create FFPE samples with four different pre-tissue proc ...
DOI:
10.1186/s13059-022-02709-8