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cellviability, etc ; DNA damage; Drosophila melanogaster; dose response; eclosion; fat body; fecundity; genes; hemocytes; metallothionein; nanosilver; pupation; sequence analysis; toxicity; China; Show all 15 Subjects
Abstract:
... The widely use of silver nanoparticles (AgNPs) as antimicrobial agents gives rise to potential environmental risks. AgNPs exposure have been reported to cause toxicity in animals. Nevertheless, the known mechanisms of AgNPs toxicity are still limited. In this study, we systematically investigated the toxicity of AgNPs exposure using Drosophila melanogaster. We show here that AgNPs significantly de ...
cellviability, etc ; curcumin; drugs; macrophages; monocytes; Show all 5 Subjects
Abstract:
... Based on the free drug hypothesis, we hypothesized that food compounds that bind stronger to BSA than CUR inhibit the binding between BSA and CUR, and that this results in an increase of the cellular uptake and physiological activities of CUR. To verify this hypothesis, food compounds that bind stronger to BSA than CUR were identified. When THP-1 monocytes were co-treated with the identified compo ...
cellviability, etc ; Citrus reticulata; antioxidant activity; polymethoxyflavones; Show all 4 Subjects
Abstract:
... This study investigated the protective effects of aged Pericarpium Citri Reticulatae ‘Chachi’ (PCR-C) on tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in HepG2 cells. According to HPLC analysis, PCR-C aged 10 years (PCR-C10) had the highest flavonoids content, especially polymethoxyflavones (PMFs), compared with the fresh peel of Citrus reticulata cv. ‘Chachiensis’ and PCR-C aged 1, 3, ...
cellviability, etc ; cell structures; cortex; polymerization; polymers; Show all 5 Subjects
Abstract:
... Conducting polymer has been directly polymerized around living neural cells or in the cortex with the aim of creating an intimate contact between implantable electrical devices and electrogenetic cells. The long term cellular effect after conductive polymer coating, a critical issue for practical applications, has not been reported. In this study, poly(3,4-ethylenedioxythiophene) PEDOT was directl ...
cellviability, etc ; bacteriophages; death; morbidity; public health; Show all 5 Subjects
Abstract:
... Staphylococcus aureus is a serious threat to public health. S. aureus infection can cause acute or long-term persistent infections that are often resistant to antibiotics and are associated with high morbidity and death. Understanding the defensive systems of S. aureus can help clinicians make the best use of antimicrobial drugs and can also help with antimicrobial stewardship. The mechanisms and ...
cellviability, etc ; biochemical pathways; endosymbionts; evolution; labor; organelles; Show all 6 Subjects
Abstract:
... Eukaryotic organelles supposedly evolved from their bacterial ancestors because of their benefits to host cells. However, organelles are quite often retained, even when the beneficial metabolic pathway is lost, due to something other than the original beneficial function. The organellar function essential for cell survival is, in the end, the result of organellar evolution, particularly losses of ...
cellviability, etc ; biotechnology; mammals; manufacturing; plasmids; polyethyleneimine; transfection; Show all 7 Subjects
Abstract:
... The transient transfection of mammalian cells is a rapid and versatile platform for the manufacture of recombinant proteins, but industrial processes depend on reliable scalability and efficient conversion from adherent to suspension cell cultures. Here we describe the optimized transfection of HEK 293T cells in both culture formats. DMEM was the best transfection medium for adherent HEK 293T cell ...
cellviability, etc ; autophagy; cancer therapy; caspase-3; glucose; starvation; Show all 6 Subjects
Abstract:
... Cancer cells are metabolically altered cells characterized by higher glucose uptake. Understanding cancer cells is vital in cancer therapy. We aimed to analyze glucose starvation effect on cell viability parameters in healthy (BEAS-2B) and cancer (A549) cells. We analyzed mTOR, p21, caspase-3, beclin-1, ATG-12 mRNA levels in cells after 8 and 24 h starvations. We also counted senescent cells post- ...
cellviability, etc ; Rhodosporidium toruloides; Tetradesmus; flow cytometry; metabolism; microalgae; wastewater; yeasts; Show all 8 Subjects
Abstract:
... Brewery wastewater (BWW) is an appealing low-cost substrate for the production of single cell oils by oleaginous microorganisms. However, it may contain inhibitor compounds that may affect the microbial metabolism. This work investigated, for the first time, the presence of potential inhibitor compounds in primary brewery wastewater (PBWW) and secondary brewery wastewater (SBWW) for the pure and m ...
cellviability, etc ; biocompatible materials; cold; cytotoxicity; homogenization; microstructure; plasticity; tensile strength; Show all 8 Subjects
Abstract:
... Ti–26Nb–2Fe–(0, 2, 4, 6, 8)Sn alloys were prepared by arc melting and subjected to homogenization, cold rolling, and solution treatment. The β phase stability of the alloys increased with the addition of Sn. Ti–26Nb–2Fe comprised ω + β phases, whereas a single β phase was detected in Ti–26Nb–2Fe–(2, 4, 6, 8)Sn. With an increase in Sn, the Young’s modulus first decreased from 83 GPa in Ti–26Nb–2Fe ...
cellviability, etc ; DNA; chemical bonding; guidelines; half life; immunotherapy; lipids; temperature; Show all 8 Subjects
Abstract:
... The cell surface can be engineered with synthetic DNA for various applications ranging from cancer immunotherapy to tissue engineering. However, while elegant methods such as click conjugation and lipid insertion have been developed to engineer the cell surface with DNA, little effort has been made to systematically evaluate and compare these methods. Resultantly, it is often challenging to choose ...
cellviability, etc ; apoptosis; drug therapy; humans; patients; prognosis; proteinases; research; ubiquitination; Show all 9 Subjects
Abstract:
... Chemoresistance is a major obstacle faced by oesophageal cancer patients and is synonymous with a poor prognosis. MCL1 is a pivotal member of the anti-apoptotic Bcl-2 protein family, which has been found to play an important role in cell survival, proliferation, differentiation and chemoresistance. Thus, it might be an ideal target for treating oesophageal cancer patients. Although it is known tha ...
cellviability, etc ; cell culture; cellulose nanofibers; flocculation; hepatocytes; polymers; sodium; survival rate; Show all 8 Subjects
Abstract:
... In this study, concentrated polymer brush-modified cellulose nanofibers (CNFs) with different fiber lengths were used for the flocculation of cells for systematically studying the mechanism of this unique cellular flocculation based on colloidal flocculation theory. Concentrated poly(p-styrenesulfonic acid sodium salt) brush-grafted CNF (CNF-PSSNa) with different fiber lengths were cultured with t ...
cellviability, etc ; absorbance; caspase-3; fluorescence; humans; neurodegenerative diseases; neurotoxicity; spectroscopy; therapeutics; Show all 9 Subjects
Abstract:
... Protein aggregating is known as a leading pathogenic characteristic of a wide range of neurodegenerative diseases (NDs). Preventing amyloid-β (Aβ) aggregation and uncovering the associated mechanism through the application of small bioactive compounds can be considered as a useful strategy in hampering the onset of ND. In this study, we analyzed the inhibitory effects of cirsiliol, a trihydroxy-di ...
cellviability, etc ; Rosmarinus officinalis; essential oils; ferulic acid; labdane; neuroprotective effect; rosemary; Show all 7 Subjects
Abstract:
... The aim of this study was to purify and identify new types of secondary products from Rosmarinus officinalis solid wastes after essential oil been extracted, further study their MS/MS spectrometric fragmentations and evaluate their cytoprotective effects against H₂O₂-induced oxidative damage in SH-SY5Y cells. Six labdane diterpenoids, five isopimarane diterpenoids and two ferulic acid esters were ...
cellviability, etc ; apoptosis; cardiomyocytes; hypoxia; models; myocardial infarction; plasmids; therapeutics; wnt proteins; Show all 9 Subjects
Abstract:
... Myocardial reperfusion, the effective therapy for acute myocardial infarction (AMI), commonly leads to myocardial ischemia/reperfusion (I/R) injury. The effects and functional mechanisms of LncRNA AZIN1-AS1 on myocardial I/R injury in vivo and vitro are not uncovered. In our present study, we established myocardial I/R injury model of mice and H/R injury model of cardiomyocytes and we discovered A ...
cellviability, etc ; bioinformatics; biotechnology; carcinogenesis; cell movement; non-coding RNA; toxicity testing; Show all 7 Subjects
Abstract:
... Choriocarcinoma is an aggressive gestational trophoblastic neoplasm. This study attempted to explore the biological functions and underlying mechanisms by which ropivacaine restrains the progression of choriocarcinoma. The expression of long noncoding RNA OGFRP1, microRNA-4731-5p (miR-4731-5p), and HIF3A in choriocarcinoma cells was assessed by qRT-PCR. Choriocarcinoma cells treated with ropivacai ...
cellviability, etc ; Western blotting; chemokines; pathogenesis; pharmacology; rats; rheumatoid arthritis; therapeutics; transcriptomics; Show all 9 Subjects
Abstract:
... Activated fibroblast-like synoviocyte (FLS) played a significant role in the pathogenesis and progression of rheumatoid arthritis (RA). Apigenin-4′-O-α-L-rhamnoside showed remarkable effects against RA, however, no relevant studies on pharmacology of apigenin-4′-O-α-L-rhamnoside yet, the effects and underlying molecular mechanism of apigenin-4′-O-α-L-rhamnoside on RA are still unclear. This study ...
cellviability, etc ; biocompatibility; catalytic activity; cytotoxicity; hydrophilicity; p-nitrophenol; rhodamines; solvents; surface area; Show all 9 Subjects
Abstract:
... As essential controlling parameters, the local surface area (size distribution) and polarity property of the surface molecules can determine the catalytic activity and biocompatibility directly. Here, ultrasmall palladium-based alloys (FePd, FePdCo, and FePdCu NCs) were developed to serve as artificial degradation catalysts with superhydrophilicity (SPh), biocompatibility, and reusability, which w ...
cellviability, etc ; bioprinting; crosslinking; encapsulation; extrusion; gelatin; hyaluronic acid; medicine; microfluidic technology; microgels; Show all 10 Subjects
Abstract:
... Extrusion bioprinting has been widely used to fabricate complicated and heterogeneous constructs for tissue engineering and regenerative medicine. Despite the remarkable progress acquired so far, the exploration of qualified bioinks is still challenging, mainly due to the conflicting requirements on the printability/shape-fidelity and cell viability. Herein, a new strategy is proposed to formulate ...