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1. Molecular toxicity and defense mechanisms induced by silver nanoparticles in Drosophila melanogaster

Author:
Zhidi Wang; Liying Zhang; Xing Wang
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 616-629
ISSN:
1001-0742
Subject:
cell viability,   etc   ; DNA damage; Drosophila melanogaster; dose response; eclosion; fat body; fecundity; genes; hemocytes; metallothionein; nanosilver; pupation; sequence analysis; toxicity; China;     Show all 15 Subjects
Abstract:
... The widely use of silver nanoparticles (AgNPs) as antimicrobial agents gives rise to potential environmental risks. AgNPs exposure have been reported to cause toxicity in animals. Nevertheless, the known mechanisms of AgNPs toxicity are still limited. In this study, we systematically investigated the toxicity of AgNPs exposure using Drosophila melanogaster. We show here that AgNPs significantly de ...
DOI:
10.1016/j.jes.2021.12.027

2. The inhibition of interaction with serum albumin enhances the physiological activity of curcumin by increasing its cellular uptake

Author:
Mayuko Itaya; Taiki Miyazawa; Saoussane Khalifa; Naoki Shimizu; Kiyotaka Nakagawa
Source:
Food & function 2022 v.13 no.2 pp. 639-648
ISSN:
2042-650X
Subject:
cell viability,   etc   ; curcumin; drugs; macrophages; monocytes;     Show all 5 Subjects
Abstract:
... Based on the free drug hypothesis, we hypothesized that food compounds that bind stronger to BSA than CUR inhibit the binding between BSA and CUR, and that this results in an increase of the cellular uptake and physiological activities of CUR. To verify this hypothesis, food compounds that bind stronger to BSA than CUR were identified. When THP-1 monocytes were co-treated with the identified compo ...
DOI:
10.1039/d1fo03234h

3. Aged Pericarpium Citri Reticulatae ‘Chachi’ Attenuates Oxidative Damage Induced by tert-Butyl Hydroperoxide (t-BHP) in HepG2 Cells

Author:
Qian Yu; Yexing Tao; Yuting Huang; Daniel Zogona; Ting Wu; Ruiting Liu; Siyi Pan; Xiaoyun Xu
Source:
Foods 2022 v.11 no.3 pp. -
ISSN:
2304-8158
Subject:
cell viability,   etc   ; Citrus reticulata; antioxidant activity; polymethoxyflavones;     Show all 4 Subjects
Abstract:
... This study investigated the protective effects of aged Pericarpium Citri Reticulatae ‘Chachi’ (PCR-C) on tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in HepG2 cells. According to HPLC analysis, PCR-C aged 10 years (PCR-C10) had the highest flavonoids content, especially polymethoxyflavones (PMFs), compared with the fresh peel of Citrus reticulata cv. ‘Chachiensis’ and PCR-C aged 1, 3, ...
DOI:
10.3390/foods11030273

4. In situ polymerization of conducting polymers around living neural cells: Cellular effect study

Author:
Hai-lan Chen; Dan Yang; Chun-rong Chen; Guang-zhao Tian; Dong-Hwan Kim
Source:
Colloids and surfaces 2022 v.213 pp. 112410
ISSN:
0927-7765
Subject:
cell viability,   etc   ; cell structures; cortex; polymerization; polymers;     Show all 5 Subjects
Abstract:
... Conducting polymer has been directly polymerized around living neural cells or in the cortex with the aim of creating an intimate contact between implantable electrical devices and electrogenetic cells. The long term cellular effect after conductive polymer coating, a critical issue for practical applications, has not been reported. In this study, poly(3,4-ethylenedioxythiophene) PEDOT was directl ...
DOI:
10.1016/j.colsurfb.2022.112410

5. The crafty opponent: the defense systems of Staphylococcus aureus and response measures

Author:
Hongjie Hou; Yang Li; Yuefei Jin; Shuaiyin Chen; Jinzhao Long; Guangcai Duan; Haiyan Yang
Source:
Folia microbiologica 2022 v.67 no.2 pp. 233-243
ISSN:
0015-5632
Subject:
cell viability,   etc   ; bacteriophages; death; morbidity; public health;     Show all 5 Subjects
Abstract:
... Staphylococcus aureus is a serious threat to public health. S. aureus infection can cause acute or long-term persistent infections that are often resistant to antibiotics and are associated with high morbidity and death. Understanding the defensive systems of S. aureus can help clinicians make the best use of antimicrobial drugs and can also help with antimicrobial stewardship. The mechanisms and ...
DOI:
10.1007/s12223-022-00954-9

6. Organellar Evolution: A Path from Benefit to Dependence

Author:
Miroslav Oborník
Source:
Microorganisms 2022 v.10 no.1 pp. -
ISSN:
2076-2607
Subject:
cell viability,   etc   ; biochemical pathways; endosymbionts; evolution; labor; organelles;     Show all 6 Subjects
Abstract:
... Eukaryotic organelles supposedly evolved from their bacterial ancestors because of their benefits to host cells. However, organelles are quite often retained, even when the beneficial metabolic pathway is lost, due to something other than the original beneficial function. The organellar function essential for cell survival is, in the end, the result of organellar evolution, particularly losses of ...
DOI:
10.3390/microorganisms10010122

7. Statistical experimental designs to optimize the transient transfection of HEK 293T cells and determine a transfer criterion from adherent cells to larger-scale cell suspension cultures

Author:
Gregor Dekevic; Lars Tasto; Peter Czermak; Denise Salzig
Source:
Journal of biotechnology 2022 v.346 pp. 23-34
ISSN:
0168-1656
Subject:
cell viability,   etc   ; biotechnology; mammals; manufacturing; plasmids; polyethyleneimine; transfection;     Show all 7 Subjects
Abstract:
... The transient transfection of mammalian cells is a rapid and versatile platform for the manufacture of recombinant proteins, but industrial processes depend on reliable scalability and efficient conversion from adherent to suspension cell cultures. Here we describe the optimized transfection of HEK 293T cells in both culture formats. DMEM was the best transfection medium for adherent HEK 293T cell ...
DOI:
10.1016/j.jbiotec.2022.01.004

8. Effect of Glucose Starvation on Cell Viability Parameters in A549 and BEAS-2B Cell Lines

Author:
B. G. Çetiner; M. Y. Terzi
Source:
Cytology and genetics 2022 v.56 no.1 pp. 66-76
ISSN:
0095-4527
Subject:
cell viability,   etc   ; autophagy; cancer therapy; caspase-3; glucose; starvation;     Show all 6 Subjects
Abstract:
... Cancer cells are metabolically altered cells characterized by higher glucose uptake. Understanding cancer cells is vital in cancer therapy. We aimed to analyze glucose starvation effect on cell viability parameters in healthy (BEAS-2B) and cancer (A549) cells. We analyzed mTOR, p21, caspase-3, beclin-1, ATG-12 mRNA levels in cells after 8 and 24 h starvations. We also counted senescent cells post- ...
DOI:
10.3103/S0095452722010054

9. Impact of brewery wastewater inhibitors in pure and mixed cultures of the yeast Rhodosporidium toruloides NCYC 921 and the microalga Tetradesmus obliquus ACOI 204/07

Author:
Carla Dias; José A.L. Santos; Alberto Reis; Teresa Lopes da Silva
Source:
Biochemical engineering journal 2022 v.185 pp. 108518
ISSN:
1369-703X
Subject:
cell viability,   etc   ; Rhodosporidium toruloides; Tetradesmus; flow cytometry; metabolism; microalgae; wastewater; yeasts;     Show all 8 Subjects
Abstract:
... Brewery wastewater (BWW) is an appealing low-cost substrate for the production of single cell oils by oleaginous microorganisms. However, it may contain inhibitor compounds that may affect the microbial metabolism. This work investigated, for the first time, the presence of potential inhibitor compounds in primary brewery wastewater (PBWW) and secondary brewery wastewater (SBWW) for the pure and m ...
DOI:
10.1016/j.bej.2022.108518

10. Microstructure, mechanical properties, and cytotoxicity of low Young’s modulus Ti–Nb–Fe–Sn alloys

Author:
Qiang Li; Tengfei Liu; Junjie Li; Chao Cheng; Mitsuo Niinomi; Kenta Yamanaka; Akihiko Chiba; Takayoshi Nakano
Source:
Journal of materials science 2022 v.57 no.9 pp. 5634-5644
ISSN:
0022-2461
Subject:
cell viability,   etc   ; biocompatible materials; cold; cytotoxicity; homogenization; microstructure; plasticity; tensile strength;     Show all 8 Subjects
Abstract:
... Ti–26Nb–2Fe–(0, 2, 4, 6, 8)Sn alloys were prepared by arc melting and subjected to homogenization, cold rolling, and solution treatment. The β phase stability of the alloys increased with the addition of Sn. Ti–26Nb–2Fe comprised ω + β phases, whereas a single β phase was detected in Ti–26Nb–2Fe–(2, 4, 6, 8)Sn. With an increase in Sn, the Young’s modulus first decreased from 83 GPa in Ti–26Nb–2Fe ...
DOI:
10.1007/s10853-022-06984-5

11. Synthetic DNA for Cell Surface Engineering: Experimental Comparison between Click Conjugation and Lipid Insertion in Terms of Cell Viability, Engineering Efficiency, and Displaying Stability

Author:
Xuelin Wang; Connie Wen; Brandon Davis; Peng Shi; Lidya Abune; Kyungsene Lee; Cheng Dong; Yong Wang
Source:
ACS applied materials & interfaces 2022 v.14 no.3 pp. 3900-3909
ISSN:
1944-8252
Subject:
cell viability,   etc   ; DNA; chemical bonding; guidelines; half life; immunotherapy; lipids; temperature;     Show all 8 Subjects
Abstract:
... The cell surface can be engineered with synthetic DNA for various applications ranging from cancer immunotherapy to tissue engineering. However, while elegant methods such as click conjugation and lipid insertion have been developed to engineer the cell surface with DNA, little effort has been made to systematically evaluate and compare these methods. Resultantly, it is often challenging to choose ...
DOI:
10.1021/acsami.1c22774

12. The deubiquitinating enzyme USP20 regulates the stability of the MCL1 protein

Author:
Jinan Feng; Pengyang Liu; Xiaonan Li; Dian Zhang; Hanbin Lin; Zhenzhu Hou; Cairu Guo; Yujie Niu; Bingyu Dai; Ouyang Wang; Min Qi; Huirui Wang; Haitao Zhou
Source:
Biochemical and biophysical research communications 2022 v.593 pp. 122-128
ISSN:
0006-291X
Subject:
cell viability,   etc   ; apoptosis; drug therapy; humans; patients; prognosis; proteinases; research; ubiquitination;     Show all 9 Subjects
Abstract:
... Chemoresistance is a major obstacle faced by oesophageal cancer patients and is synonymous with a poor prognosis. MCL1 is a pivotal member of the anti-apoptotic Bcl-2 protein family, which has been found to play an important role in cell survival, proliferation, differentiation and chemoresistance. Thus, it might be an ideal target for treating oesophageal cancer patients. Although it is known tha ...
DOI:
10.1016/j.bbrc.2022.01.019

13. Cellular Flocculation Using Concentrated Polymer Brush-Modified Cellulose Nanofibers with Different Fiber Lengths

Author:
Xida Yuan; Punnida Nonsuwan; Miwako Shobo; Robin Rajan; Tomohiko Yamazaki; Keita Sakakibara; Kazuaki Matsumura; Chiaki Yoshikawa
Source:
Biomacromolecules 2022 v.23 no.3 pp. 1101-1111
ISSN:
1526-4602
Subject:
cell viability,   etc   ; cell culture; cellulose nanofibers; flocculation; hepatocytes; polymers; sodium; survival rate;     Show all 8 Subjects
Abstract:
... In this study, concentrated polymer brush-modified cellulose nanofibers (CNFs) with different fiber lengths were used for the flocculation of cells for systematically studying the mechanism of this unique cellular flocculation based on colloidal flocculation theory. Concentrated poly(p-styrenesulfonic acid sodium salt) brush-grafted CNF (CNF-PSSNa) with different fiber lengths were cultured with t ...
DOI:
10.1021/acs.biomac.1c01424

14. Cirsiliol mitigates Aβ fibrillation and underlying membrane-leakage associated neurotoxicity: A possible implication in the treatment of neurodegenerative disease

Author:
Hongyu Hu; Wenjun Du; Weidong Zhang; Jun Fang
Source:
International journal of biological macromolecules 2022 v.213 pp. 915-922
ISSN:
0141-8130
Subject:
cell viability,   etc   ; absorbance; caspase-3; fluorescence; humans; neurodegenerative diseases; neurotoxicity; spectroscopy; therapeutics;     Show all 9 Subjects
Abstract:
... Protein aggregating is known as a leading pathogenic characteristic of a wide range of neurodegenerative diseases (NDs). Preventing amyloid-β (Aβ) aggregation and uncovering the associated mechanism through the application of small bioactive compounds can be considered as a useful strategy in hampering the onset of ND. In this study, we analyzed the inhibitory effects of cirsiliol, a trihydroxy-di ...
DOI:
10.1016/j.ijbiomac.2022.06.023

15. Labdane and isopimarane diterpenoids from Rosmarinus officinalis solid wastes: MS/MS spectrometric fragmentations and neuroprotective effect

Author:
Xinglong Chen; Qingyi Luo; Weiyan Hu; Jijun Chen; Rongping Zhang
Source:
Industrial crops and products 2022 v.177 pp. 114441
ISSN:
0926-6690
Subject:
cell viability,   etc   ; Rosmarinus officinalis; essential oils; ferulic acid; labdane; neuroprotective effect; rosemary;     Show all 7 Subjects
Abstract:
... The aim of this study was to purify and identify new types of secondary products from Rosmarinus officinalis solid wastes after essential oil been extracted, further study their MS/MS spectrometric fragmentations and evaluate their cytoprotective effects against H₂O₂-induced oxidative damage in SH-SY5Y cells. Six labdane diterpenoids, five isopimarane diterpenoids and two ferulic acid esters were ...
DOI:
10.1016/j.indcrop.2021.114441

16. LncRNA AZIN1-AS1 ameliorates myocardial ischemia–reperfusion injury by targeting miR-6838-5p/WNT3A axis to activate Wnt-β/catenin signaling pathway

Author:
Guoming Zhang; Licheng Ding; Guangfeng Sun; Zhixian Liu; Weimei Ou; Bin Wang; Yuanyuan Sun
Source:
In vitro cellular & developmental biology 2022 v.58 no.1 pp. 54-68
ISSN:
1071-2690
Subject:
cell viability,   etc   ; apoptosis; cardiomyocytes; hypoxia; models; myocardial infarction; plasmids; therapeutics; wnt proteins;     Show all 9 Subjects
Abstract:
... Myocardial reperfusion, the effective therapy for acute myocardial infarction (AMI), commonly leads to myocardial ischemia/reperfusion (I/R) injury. The effects and functional mechanisms of LncRNA AZIN1-AS1 on myocardial I/R injury in vivo and vitro are not uncovered. In our present study, we established myocardial I/R injury model of mice and H/R injury model of cardiomyocytes and we discovered A ...
DOI:
10.1007/s11626-022-00646-1

17. Ropivacaine Retards the Viability, Migration, and Invasion of Choriocarcinoma Cells by Regulating the Long Noncoding RNA OGFRP1/MicroRNA-4731-5p/HIF3A Axis

Author:
Yaojun Lu; Chen Yang; Le Zhang; Juan Ding
Source:
Molecular biotechnology 2022 v.64 no.5 pp. 499-509
ISSN:
1073-6085
Subject:
cell viability,   etc   ; bioinformatics; biotechnology; carcinogenesis; cell movement; non-coding RNA; toxicity testing;     Show all 7 Subjects
Abstract:
... Choriocarcinoma is an aggressive gestational trophoblastic neoplasm. This study attempted to explore the biological functions and underlying mechanisms by which ropivacaine restrains the progression of choriocarcinoma. The expression of long noncoding RNA OGFRP1, microRNA-4731-5p (miR-4731-5p), and HIF3A in choriocarcinoma cells was assessed by qRT-PCR. Choriocarcinoma cells treated with ropivacai ...
DOI:
10.1007/s12033-021-00429-1

18. Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK signaling pathway

Author:
Dan Cao; Qiqi Fan; Zhiqi Li; Meilin Chen; Yangyu Jiang; Ruichao Lin; Jian Li; Chongjun Zhao
Source:
Phytomedicine 2022 v.102 pp. 154201
ISSN:
0944-7113
Subject:
cell viability,   etc   ; Western blotting; chemokines; pathogenesis; pharmacology; rats; rheumatoid arthritis; therapeutics; transcriptomics;     Show all 9 Subjects
Abstract:
... Activated fibroblast-like synoviocyte (FLS) played a significant role in the pathogenesis and progression of rheumatoid arthritis (RA). Apigenin-4′-O-α-L-rhamnoside showed remarkable effects against RA, however, no relevant studies on pharmacology of apigenin-4′-O-α-L-rhamnoside yet, the effects and underlying molecular mechanism of apigenin-4′-O-α-L-rhamnoside on RA are still unclear. This study ...
DOI:
10.1016/j.phymed.2022.154201

19. A rational synthesis of ultrasmall palladium-based alloys with superhydrophilicity as biocompatible agents and recyclable catalysts

Author:
Shiyue Chen; Xiaoxiao He; Xulei Yuan; Zhenyu Wang; Teng Wang; Chengdian He; Ximu Zhang; Xiang Mao
Source:
RSC advances 2022 v.12 no.13 pp. 8102-8107
ISSN:
2046-2069
Subject:
cell viability,   etc   ; biocompatibility; catalytic activity; cytotoxicity; hydrophilicity; p-nitrophenol; rhodamines; solvents; surface area;     Show all 9 Subjects
Abstract:
... As essential controlling parameters, the local surface area (size distribution) and polarity property of the surface molecules can determine the catalytic activity and biocompatibility directly. Here, ultrasmall palladium-based alloys (FePd, FePdCo, and FePdCu NCs) were developed to serve as artificial degradation catalysts with superhydrophilicity (SPh), biocompatibility, and reusability, which w ...
DOI:
10.1039/d2ra00266c

20. Assembling Microgels via Dynamic Cross-Linking Reaction Improves Printability, Microporosity, Tissue-Adhesion, and Self-Healing of Microgel Bioink for Extrusion Bioprinting

Author:
Qi Feng; Dingguo Li; Qingtao Li; Haofei Li; Zetao Wang; Shuangli Zhu; Zefeng Lin; Xiaodong Cao; Hua Dong
Source:
ACS applied materials & interfaces 2022 v.14 no.13 pp. 15653-15666
ISSN:
1944-8252
Subject:
cell viability,   etc   ; bioprinting; crosslinking; encapsulation; extrusion; gelatin; hyaluronic acid; medicine; microfluidic technology; microgels;     Show all 10 Subjects
Abstract:
... Extrusion bioprinting has been widely used to fabricate complicated and heterogeneous constructs for tissue engineering and regenerative medicine. Despite the remarkable progress acquired so far, the exploration of qualified bioinks is still challenging, mainly due to the conflicting requirements on the printability/shape-fidelity and cell viability. Herein, a new strategy is proposed to formulate ...
DOI:
10.1021/acsami.2c01295