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1. A cell viability assessment method based on area-normalized impedance spectrum (ANIS)

Author:
Zhang, Rongbiao; Wei, Mingji; Chen, Shuohuan; Li, Guoxiao; Zhang, Fei; Yang, Ning; Huang, Linkui
Source:
Biosensors & bioelectronics 2018 v.110 pp. 193-200
ISSN:
0956-5663
Subject:
cell viability,   etc   ; biosensors; models;     Show all 3 Subjects
Abstract:
... Impedance measurement of cells using electric cell-substrate impedance sensing (ECIS) is widely accepted as an effective method to assess cell status. However, the sensitive frequency drifts over time with the changes of culture condition according to the built circuit model and experimental results. The area-normalized impedance spectrum (ANIS) method, which uses normalized area of impedance spec ...
DOI:
10.1016/j.bios.2018.03.041

2. Characterization of trimethyl chitosan/polyethylene glycol derivatized chitosan blend as an injectable and degradable antimicrobial delivery system

Author:
Boles, Logan R.; Bumgardner, Joel D.; Fujiwara, Tomoko; Haggard, Warren O.; Guerra, Fernanda D.; Jennings, Jessica A.
Source:
International journal of biological macromolecules 2019 v.133 pp. 372-381
ISSN:
0141-8130
Subject:
cell viability,   etc   ; amikacin; chitosan; fibroblasts; orthopedics; polyethylene glycol; vancomycin;     Show all 7 Subjects
Abstract:
... Advanced local delivery systems are needed as adjunctive treatments for severe injuries with high infection rates, such as open fractures. Chitosan systems have been investigated as antimicrobial local delivery systems for orthopaedic infection but possess mismatches between elution and degradation properties. Derivatives of chitosan were chosen that have enhanced swelling ratios or tailorable deg ...
DOI:
10.1016/j.ijbiomac.2019.04.075

3. Beyond the vicious cycle: The role of innate osteoimmunity, automimicry and tumor-inherent changes in dictating bone metastasis

Author:
Owen, Katie L.; Parker, Belinda S.
Source:
Molecular immunology 2019 v.110 pp. 57-68
ISSN:
0161-5890
Subject:
cell viability,   etc   ; cell growth; metastasis; mortality; neoplasm cells; patients; therapeutics;     Show all 7 Subjects
Abstract:
... Bone metastasis is a fatal consequence of a subset of solid malignancies that fail to respond to conventional therapies. While a myriad of factors contribute to osteotropism and disseminated cell survival and outgrowth in bone, efforts to inhibit tumor cell growth in the bone-metastatic niche have largely relied on measures that disrupt the bi-directional interactions between bone resident and tum ...
DOI:
10.1016/j.molimm.2017.11.023

4. Inhibition of peroxiredoxin 2 suppresses Wnt/β-catenin signaling in gastric cancer

Author:
Lee, Tae Hyeong; Jin, Jun-O.; Yu, Ki Jin; Kim, Hee Sung; Lee, Peter Chang-Whan
Source:
Biochemical and biophysical research communications 2019 v.512 no.2 pp. 250-255
ISSN:
0006-291X
Subject:
cell viability,   etc   ; fluorouracil; humans; hydrogen peroxide; peroxiredoxin; proteins; stomach neoplasms;     Show all 7 Subjects
Abstract:
... Gastric cancer (GC) is the fourth most common type of malignant tumor that affects humans worldwide, but few targeted therapies for it have been considered that are based on redox systems. Peroxiredoxin2 (Prx2) functions as a reactive oxygen species (ROS)-mediated signaling regulator that controls H2O2 in mammalian cells, and it is involved in the survival of various malignant tumors. In human GC ...
DOI:
10.1016/j.bbrc.2019.03.039

5. Antioxidant activity of proteins extracted from red alga dulse harvested in Japan

Author:
Sato, Naoto; Furuta, Tomoe; Takeda, Tomoyuki; Miyabe, Yoshikatsu; Ura, Kazuhiro; Takagi, Yasuaki; Yasui, Hajime; Kumagai, Yuya; Kishimura, Hideki
Source:
Journal of food biochemistry 2019 v.43 no.2 pp. e12709
ISSN:
0145-8884
Subject:
cell viability,   etc   ; Palmaria; antioxidant activity; apoproteins; phycoerythrin; viability assays; Japan;     Show all 7 Subjects
Abstract:
... In this study, we investigated antioxidant activity of proteins from the red alga dulse (Palmaria sp.) harvested in Hokkaido, Japan. The dulse proteins that contain phycoerythrin (PE) as the main component showed a high radical scavenging activity. To clarify the key constituent of antioxidant activity in dulse proteins, we prepared recombinant dulse PE β‐subunit (rPEβ) (apoprotein) and chromophor ...
DOI:
10.1111/jfbc.12709

6. The effect of hyperosmolality application time on production, quality, and biopotency of monoclonal antibodies produced in CHO cell fed-batch and perfusion cultures

Author:
Qin, Jinyan; Wu, Xiang; Xia, Zhigang; Huang, Zheng; Zhang, Ying; Wang, Yanchao; Fu, Qiang; Zheng, Chen
Source:
Applied microbiology and biotechnology 2019 v.103 no.3 pp. 1217-1229
ISSN:
0175-7598
Subject:
cell viability,   etc   ; application timing; cytotoxicity; monoclonal antibodies; sodium chloride;     Show all 5 Subjects
Abstract:
... Hyperosmolality has been commonly investigated due to its effects on the production and quality characteristics of monoclonal antibodies (mAbs) produced in CHO cell fed-batch cultures. However, the application of hyperosmolality at different times and its effect on biopotency have seldom been researched, especially in perfusion culture. In our study, different degrees of hyperosmolality induced by ...
DOI:
10.1007/s00253-018-9555-7

7. Assessment of commercial probiotic products in China for labelling accuracy and probiotic characterisation of selected isolates

Author:
Chen, Tingtao; Wu, Qinglong; Zhou, Haiting; Deng, Kan; Wang, Xin; Meng, Fanjing; Yang, Shaoguo; Wang, Xiaolei; Shah, Nagendra P; Wei, Hua
Source:
International journal of dairy technology 2017 v.70 no.1 pp. 119-126
ISSN:
1364-727X
Subject:
cell viability,   etc   ; probiotics; China;     Show all 3 Subjects
Abstract:
... Microbial populations in probiotic supplements in China were investigated using the plate count method and PCR–DGGE technique. Our results indicated that total viable cells in most probiotic products could meet the minimum requirement of the Chinese National Standards (10⁶ CFU/mL); however, total viable cell counts of the species detected in the probiotic products did not correspond with the cell ...
DOI:
10.1111/1471-0307.12331

8. Cubic membrane formation supports cell survival of amoeba Chaos under starvation-induced stress

Author:
Chong, Ketpin; Almsherqi, ZakariaA.; Shen, Han-Ming; Deng, Yuru
Source:
Protoplasma 2018 v.255 no.2 pp. 517-525
ISSN:
0033-183X
Subject:
cell viability,   etc   ; adenosine triphosphate; autophagy; mitochondria; rapamycin;     Show all 5 Subjects
Abstract:
... Cubic membranes (CM) are highly organized membrane structures found in biological systems. They are mathematically well defined and reveal a three-dimensional nano-periodic structure with cubic symmetry. These membrane arrangements are frequently induced in cells under stress, disease conditions, or upon viral infection. In this study, we investigated CM formation in the mitochondria of amoeba Cha ...
DOI:
10.1007/s00709-017-1169-x

9. ESCRTing Necroptosis

Author:
Guo, Hongyan; Kaiser, William J.
Source:
Cell 2017 v.169 pp. 186-187
ISSN:
0092-8674
Subject:
cell viability,   etc   ; apoptosis; plasma membrane;     Show all 3 Subjects
Abstract:
... Necroptosis is a highly inflammatory form of programmed cell death that results from MLKL-mediated disruption of the cell membrane. In this issue of Cell, Gong et al. challenge the notion that MLKL activation is a point of no return by identifying mechanisms to counterbalance necroptosis, sustain plasma membrane integrity, and prolong cell viability. ...
DOI:
10.1016/j.cell.2017.03.030

10. Exosomal miR-196a derived from cancer-associated fibroblasts confers cisplatin resistance in head and neck cancer through targeting CDKN1B and ING5

Author:
Qin, Xing; Guo, Haiyan; Wang, Xiaoning; Zhu, Xueqin; Yan, Ming; Wang, Xu; Xu, Qin; Shi, Jianbo; Lu, Eryi; Chen, Wantao; Zhang, Jianjun
Source:
Genome biology 2019 v.20 no.1 pp. 12
ISSN:
1474-760X
Subject:
cell viability,   etc   ; cisplatin; exosomes; fibroblasts; head and neck neoplasms; neoplasm cells; ribonucleoproteins; therapeutics;     Show all 8 Subjects
Abstract:
... BACKGROUND: Cisplatin resistance is a major challenge for advanced head and neck cancer (HNC). Understanding the underlying mechanisms and developing effective strategies against cisplatin resistance are highly desired in the clinic. However, how tumor stroma modulates HNC growth and chemoresistance is unclear. RESULTS: We show that cancer-associated fibroblasts (CAFs) are intrinsically resistant ...
DOI:
10.1186/s13059-018-1604-0

11. Effects of fluid-dynamic conditions in Shimwellia blattae (p424IbPSO) cultures in stirred tank bioreactors: Hydrodynamic stress and change of metabolic routes by oxygen availability

Author:
Acedos, Miguel G.; Hermida, Andrea; Gomez, Emilio; Santos, Victoria E.; Garcia-Ochoa, Felix
Source:
Biochemical engineering journal 2019 v.149 pp. 107238
ISSN:
1369-703X
Subject:
cell viability,   etc   ; agitation; biomass; bioreactors; dissolved oxygen; hydrodynamics; kinetics; metabolites; oxygen;     Show all 9 Subjects
Abstract:
... Cultures of Shimwellia blattae (p424IbPSO) under different fluid dynamic conditions have been studied. Runs have been performed in a STBR changing the stirrer speed from 100 to 1200 rpm. Growth rates have been determined measuring biomass concentration with time. A kinetic model has been fitted to experimental data. Results show that hydrodynamic stress appears in experiments performed at high agi ...
DOI:
10.1016/j.bej.2019.107238

12. Phenotypic and genomic analysis of Zymomonas mobilis ZM4 mutants with enhanced ethanol tolerance

Author:
Carreón-Rodríguez, Ofelia E.; Gutiérrez-Ríos, Rosa María; Acosta, José L.; Martinez, Alfredo; Cevallos, Miguel A.
Source:
Biotechnology reports 2019 v.23 pp. e00328
ISSN:
2215-017X
Subject:
cell viability,   etc   ; Zymomonas mobilis; alleles; biotechnology; ethanol; genomics; genotyping; mutants; mutation; phenotype; solvents;     Show all 11 Subjects
Abstract:
... Zymomonas mobilis ZM4 is an ethanol-producing microbe that is constitutively tolerant to this solvent. For a better understanding of the ethanol tolerance phenomenon we obtained and characterized two ZM4 mutants (ER79ap and ER79ag) with higher ethanol tolerance than the wild-type. Mutants were evaluated in different ethanol concentrations and this analysis showed that mutant ER79ap was more tolera ...
DOI:
10.1016/j.btre.2019.e00328

13. The formation and bioactivities of green substances in Chrysanthemum morifolium tea

Author:
Wang, Yuxiao; Xu, Zhenzhen; Wen, Xin; Li, Mo; Pang, Sicheng; Huang, Yuqi; Ni, Yuanying
Source:
Food chemistry 2019 v.286 pp. 268-274
ISSN:
0308-8146
Subject:
cell viability,   etc   ; Chrysanthemum morifolium; Fourier transform infrared spectroscopy; antioxidant activity; brewing; color; cytotoxicity; glycosides; tea;     Show all 9 Subjects
Abstract:
... One interesting phenomenon of Chrysanthemum morifolium tea is its formation of green or dark green color after hours of brewing. We investigated the greening reaction and its bioactivities, including an analysis of the green compounds. Results showed that the green color was due to a decrease in the L* (lightness), b* (yellowness/blueness), chroma values and an increase in hue angle. The green sub ...
DOI:
10.1016/j.foodchem.2019.02.026

14. Prevention and treatment effects of edible berries for three deadly diseases: Cardiovascular disease, cancer and diabetes

Author:
Yang, Hua; Tian, Tiantian; Wu, Dianhui; Guo, Dejun; Lu, Jian
Source:
Critical reviews in food science and nutrition 2019 v.59 no.12 pp. 1903-1912
ISSN:
1549-7852
Subject:
cell viability,   etc   ; cardiovascular diseases; diabetes; human health; neoplasms; phytochemicals; signal transduction; small fruits;     Show all 8 Subjects
Abstract:
... Cardiovascular disease (CVD), cancer and diabetes are serious threat to human health and more and more aroused people's attention. It is important to find the safe and effective prevention and treatment methods for the three deadly diseases. At present, a generally attention in the possible positive effects of edible berries for the three deadly diseases has been noted. Berry phytochemical compoun ...
DOI:
10.1080/10408398.2018.1432562

15. Elucidating Peptidoglycan Structure: An Analytical Toolset

Author:
Porfírio, Sara; Carlson, Russell W.; Azadi, Parastoo
Source:
Trends in microbiology 2019 v.27 no.7 pp. 607-622
ISSN:
0966-842X
Subject:
cell viability,   etc   ; bacteria; bioinformatics; biosynthesis; cell walls; drugs; high performance liquid chromatography; pathogenesis; peptidoglycans; pharmacology; turgor;     Show all 11 Subjects
Abstract:
... Peptidoglycan (PG) is a ubiquitous structural polysaccharide of the bacterial cell wall, essential in preserving cell integrity by withstanding turgor pressure. Any change that affects its biosynthesis or degradation will disturb cell viability, therefore PG is one of the main targets of antimicrobial drugs. Considering its major role in cell structure and integrity, the study of PG is of utmost r ...
DOI:
10.1016/j.tim.2019.01.009

16. Modeling of Shigella flexneri inactivation by combination of ultrasound, pH and nisin

Author:
Freitas, Leonardo Luiz de; Prudêncio, Cláudia Vieira; Peña, Wilmer Edgard Luera; Vanetti, Maria Cristina Dantas
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2019 v.109 pp. 40-46
ISSN:
0023-6438
Subject:
cell viability,   etc   ; Shigella flexneri; adenosine triphosphate; death; models; nisin; pH; pathogens; response surface methodology; storage conditions;     Show all 10 Subjects
Abstract:
... The Response Surface Methodology (RSM) was used to establish a predictive model of inactivation of S. flexneri using ultrasound time of 5–20 min, pH of 4.0–7.0 and nisin concentration of 29.1–291.1 μM as independent variables. In addition, a survival of S. flexneri at 7 °C for 120 h after treatment with ultrasound and nisin was evaluated as well as the ATP leakage. The response surface model (RS m ...
DOI:
10.1016/j.lwt.2019.03.045

17. Dual-channel fluorescent probe bearing two-photon activity for cell viability monitoring

Author:
ZhaoThese authors contributed equally to this work., Yanqian; Zhang, Chengkai; Liu, Jiejie; Li, Dandan; Tian, Xiaohe; Wang, Aidong; Li, Shengli; Wu, Jieying; Tian, Yupeng
Source:
Journal of materials chemistry B 2019 v.7 no.23 pp. 3633-3638
ISSN:
2050-7518
Subject:
cell viability,   etc   ; biocompatibility; fluorescence microscopy; fluorescent dyes; image analysis; mitochondria; moieties; monitoring; oxygen; triazoles; water solubility;     Show all 11 Subjects
Abstract:
... The search for a highly sensitive and efficient method to monitor cell viability is a high-priority yet challenging. In this study, two chromophores, 1OC and 2OC, were rationally designed and fabricated to achieve excellent biocompatibility and two-photon activity in the near-infrared region. Fortunately, because of the introduction of water-soluble moieties and biocompatible triazole groups that ...
DOI:
10.1039/c9tb00512a

18. Assessment of fed-batch cultivation strategies for an inducible CHO cell line

Author:
Mellahi, Kahina; Brochu, Denis; Gilbert, Michel; Perrier, Michel; Ansorge, Sven; Durocher, Yves; Henry, Olivier
Source:
Journal of biotechnology 2019 v.298 pp. 45-56
ISSN:
0168-1656
Subject:
cell viability,   etc   ; antibodies; bioreactors; cell growth; cell lines; developmental stages; glucose; hypothermia; overfeeding; risk;     Show all 10 Subjects
Abstract:
... In order to maximize cell growth and productivity for an inducible CHO cell line expressing rituximab, various fed-batch culture strategies were investigated. In each case, the performance was evaluated for cultures induced at moderate and high cell density conditions (4 × 106 and 10 × 106 cells/mL) to assess the impact of the timing of induction. We first demonstrate the importance of starting th ...
DOI:
10.1016/j.jbiotec.2019.04.005

19. A 3D cell printed muscle construct with tissue-derived bioink for the treatment of volumetric muscle loss

Author:
Choi, Yeong-Jin; Jun, Young-Joon; Kim, Dong Yeon; Yi, Hee-Gyeong; Chae, Su-Hun; Kang, Junsu; Lee, Juyong; Gao, Ge; Kong, Jeong-Sik; Jang, Jinah; Chung, Wan Kyun; Rhie, Jong-Won; Cho, Dong-Woo
Source:
Biomaterials 2019 v.206 pp. 160-169
ISSN:
0142-9612
Subject:
cell viability,   etc   ; animal models; biomimetics; bioprinting; extracellular matrix; hypoxia; innervation; muscles;     Show all 8 Subjects
Abstract:
... Volumetric muscle loss (VML) is an irrecoverable injury associated with muscle loss greater than 20%. Although hydrogel-based 3D engineered muscles and the decellularized extracellular matrix (dECM) have been considered for VML treatment, they have shown limited efficacy. We established a novel VML treatment with dECM bioink using 3D cell printing technology. Volumetric muscle constructs composed ...
DOI:
10.1016/j.biomaterials.2019.03.036

20. Antioxidant and Antiproliferative Activities of Cyanidin‐3‐O‐Glucoside (C3G) Liposome in Caco‐2 Cells Cultivated in 2D and 3D Cell Culture Models

Author:
Liang, Tisong; Tao, Qingfeng; Guan, Rongfa; Cao, Guozhou; Shen, Haitao; Liu, Zhenfeng; Xia, Qile
Source:
Journal of food science 2019 v.84 no.6 pp. 1638-1645
ISSN:
0022-1147
Subject:
cell viability,   etc   ; antioxidant activity; cell culture; glutathione; human cell lines; hydrogen peroxide; malondialdehyde; models; superoxide dismutase;     Show all 9 Subjects
Abstract:
... The aim of this study was to obtain adequate and detailed information about the antioxidant and antiproliferative activities of C3G and C3G liposomes in different cell culture models. The Caco‐2 cells were cultured in 2D and 3D cell culture models, the H₂O₂ was used to construct the cell damage model and then the cells treated with C3G and C3G liposomes. The antioxidant activity and antiproliferat ...
DOI:
10.1111/1750-3841.14629