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1. Effects of the Addition of Poly(ethylene Glycol) and Non-ionic Surfactants on Pretreatment, Enzymatic Hydrolysis, and Ethanol Fermentation

Author:
Cleitiane da Costa Nogueira; Carlos Eduardo de Araújo Padilha; Pedro Ferreira de Souza Filho; Everaldo Silvino dos Santos
Source:
BioEnergy research 2022 v.15 no.2 pp. 889-904
ISSN:
1939-1234
Subject:
cellulases,   etc   ; agricultural wastes; bioethanol; enzymatic hydrolysis; ethanol fermentation; ethanol production; lignin; lignocellulose; markets; octoxynol; polysorbates; research;     Show all 12 Subjects
Abstract:
... The consolidation of cellulosic ethanol on the market is fundamental to mitigate the consumption of fuels from fossil sources and to reduce the impact caused by the large generation of agro-industrial waste. In order to achieve this objective, some challenges of cellulosic ethanol technology must be overcome, including the improvement of the cellulosic ethanol production steps. Several studies pro ...
DOI:
10.1007/s12155-021-10388-9

2. Valorization of cellulose in waste paper into value-added cellobionic acid by genetically engineered Pseudomonas taetrolens

Author:
Youngchul Yoo; Yu-Ri Oh; Gyeong Tae Eom
Source:
Industrial crops and products 2022 v.186 pp. 115186
ISSN:
0926-6690
Subject:
cellulases,   etc   ; Pseudomonas taetrolens; biocatalysis; cellobiose; cellulose; genetic engineering; glucose; oxidoreductases; prices; temperature; value added; waste paper;     Show all 12 Subjects
Abstract:
... To expand the applications of waste paper (WP), we attempted to valorize waste office paper (WOP) into cellobionic acid (CBA) using a two-step biocatalytic method. First, we prepared cellobiose from WOP using commercial cellulases. To increase cellobiose production, we optimized various reaction conditions; the type of cellulases, reaction temperature, the amount of WOP, and the amount of cellulas ...
DOI:
10.1016/j.indcrop.2022.115186

3. Statistical optimization of aqueous ammonia pretreatment and enzymatic saccharification of corn stover for enhancing sugars production

Author:
Hui Zhang; Junhui Wu
Source:
Environmental technology & innovation 2022 v.26 pp. 102238
ISSN:
2352-1864
Subject:
cellulases,   etc   ; ammonia; biomass; cellulose; corn stover; environmental technology; hemicellulose; lignin; pH; particle size; polysorbates; response surface methodology; saccharification; temperature; xylose;     Show all 15 Subjects
Abstract:
... Response surface methodology (RSM) was adopted to optimize aqueous ammonia pretreatment and enzymatic saccharification of corn stover for enhancing sugars production, respectively. Pretreatment variables including pretreatment temperature, aqueous ammonia concentration and pretreatment time and saccharification variables including reaction time, enzyme loading and biomass loading were successfully ...
DOI:
10.1016/j.eti.2021.102238

4. Understanding the toxicity of lignin-derived phenolics towards enzymatic saccharification of lignocellulose for rationally developing effective in-situ mitigation strategies to maximize sugar production from lignocellulosic biorefinery

Author:
Xiaoxiao Jiang; Rui Zhai; Yu Leng; Qiufeng Deng; Mingjie Jin
Source:
Bioresource technology 2022 v.349 pp. 126813
ISSN:
0960-8524
Subject:
cellulases,   etc   ; Lewis acids; biorefining; cellulose; enzymatic hydrolysis; hydrolysates; hydrolysis; hydrophobicity; lignin; lignocellulose; models; phenolic compounds; saccharification; sugars; toxicity; wheat straw;     Show all 16 Subjects
Abstract:
... The lignin-derived phenolics are highly inhibitory toward lignocellulose enzymatic hydrolysis, while the relationship between phenolic structure and inhibitory effect is still not fully understood. In this study, the compositions of phenolics from dilute acid pretreated wheat straw were analyzed and their impact on cellulose hydrolysis was studied. With increase of pretreatment severity, more toxi ...
DOI:
10.1016/j.biortech.2022.126813

5. Integrating mild chemical pretreatments with endogenous protein supplement for complete biomass saccharification to maximize bioethanol production by enhancing cellulases adsorption in novel bioenergy Amaranthus

Author:
Meysam Madadi; Youmei Wang; Ran Zhang; Zhen Hu; Hairong Gao; Dan Zhan; Hua Yu; Qiaomei Yang; Yanting Wang; Yuanyuan Tu; Tao Xia; Liangcai Peng
Source:
Industrial crops and products 2022 v.177 pp. 114471
ISSN:
0926-6690
Subject:
cellulases,   etc   ; Amaranthus; adsorption; bioethanol; biomass; carbon; energy crops; enzymatic hydrolysis; ethanol production; fermentation; fuel production; lignocellulose; models; perennials; polymers; porosity; protein supplements; saccharification; starch; straw; yeasts;     Show all 21 Subjects
Abstract:
... Amaranthus is a fast-growing perennial plant containing large amounts of extractable green proteins and lignocellulose residues. However, it remains to explore optimal Amaranthus biomass process technology for biofuel production. In this study, we examined that mild alkali and acid pretreatments (1% NaOH, 2% H₂SO₄) were sufficient for near-complete biomass enzymatic saccharification, while 8% Amar ...
DOI:
10.1016/j.indcrop.2021.114471

6. Enzyme-assisted extraction of anthocyanins and other phenolic compounds from blackcurrant (Ribes nigrum L.) press cake: From processing to bioactivities

Author:
Daniel Granato; Marina Fidelis; Marjo Haapakoski; Amanda dos Santos Lima; Janeli Viil; Jarkko Hellström; Reelika Rätsep; Hedi Kaldmäe; Uko Bleive; Luciana Azevedo; Varpu Marjomäki; Alexander Zharkovsky; Nora Pap
Source:
Food chemistry 2022 v.391 pp. 133240
ISSN:
0308-8146
Subject:
cellulases,   etc   ; Betacoronavirus 1; Ribes nigrum; anthocyanins; anti-inflammatory activity; antioxidant activity; antiviral properties; black currants; cytotoxicity; fibroblasts; food chemistry; freeze drying; humans; lungs; lyases; mice; pectins; polyphenols;     Show all 18 Subjects
Abstract:
... The effects of commercial enzymes (pectinases, cellulases, beta-1-3-glucanases, and pectin lyases) on the recovery of anthocyanins and polyphenols from blackcurrant press cake were studied considering two solid:solvent ratios (1:10 and 1:4 w/v). β-glucanase enabled the recovery of the highest total phenolic content – 1142 mg/100 g, and the extraction of anthocyanins was similar using all enzymes ( ...
DOI:
10.1016/j.foodchem.2022.133240

7. Digestibility, bioaccessibility and bioactivity of compounds from algae

Author:
Mariana Demarco; Jaqueline Oliveira de Moraes; Ângelo Paggi Matos; Roberto Bianchini Derner; Fábio de Farias Neves; Giustino Tribuzi
Source:
Trends in food science & technology 2022 v.121 pp. 114-128
ISSN:
0924-2244
Subject:
cellulases,   etc   ; amino acids; bioactive properties; bioavailability; biomass; breads; carotenoids; cell walls; chickens; chlorophyll; digestibility; feed supplements; health promotion; homogenization; human health; humans; microalgae; phycocyanin; polyphenols; polysaccharides; sheep; yogurt;     Show all 22 Subjects
Abstract:
... Algae are aquatic organisms rich in several biocompounds, and major ones are proteins and amino acids, carbohydrates and polysaccharides, lipids and fatty acids, pigments like chlorophyll, carotenoid, and phycocyanin, vitamins, minerals, and polyphenols that exhibit multiple beneficial effects on human health. These compounds are present in high concentrations in raw algal biomass, but some of the ...
DOI:
10.1016/j.tifs.2022.02.004

8. Green biorefinery for sugar beet pulp valorisation: Microwave hydrothermal processing for pectooligosaccharides recovery and biobutanol production

Author:
Esther del Amo-Mateos; Juan C. López-Linares; M. Teresa García-Cubero; Susana Lucas; Mónica Coca
Source:
Industrial crops and products 2022 v.184 pp. 115060
ISSN:
0926-6690
Subject:
cellulases,   etc   ; Clostridium beijerinckii; biobutanol; biorefining; butanol; chemical composition; enzymatic hydrolysis; esterification; ethanol; experimental design; feedstocks; fermentation; fractionation; glucans; hot water treatment; microwave treatment; molecular weight; pectins; sugar beet pulp; temperature;     Show all 20 Subjects
Abstract:
... The complete valorisation of sugar beet pulp (SBP) through pectooligosaccharides (POS) extraction and biobutanol production was studied. A central composite experimental design was planned to optimise the time and temperature of the hydrothermal microwave-assisted extraction. 59.7% of the POS present in the SBP were recovered, and a prehydrolysate with 31.1 g POS/L was obtained under optimal condi ...
DOI:
10.1016/j.indcrop.2022.115060

9. In-situ corn fiber conversion method unlocks the role of viscosity on enhancing ethanol yield by reducing side-product glycerol

Author:
Xiujuan Li; Qiang Xiong; Minghui Wang; He Huang; Guojun Yue; Mingjie Jin
Source:
Industrial crops and products 2021 v.169 pp. 113653
ISSN:
0926-6690
Subject:
cellulases,   etc   ; bioethanol; capital; corn; energy; ethanol; ethanol production; fermentation; glucose; glycerol; transcriptome; transcriptomics; viscosity;     Show all 13 Subjects
Abstract:
... In-situ corn fiber conversion enables to greenly convert corn fiber into bioethanol with saving energy and capital expenditure. However, the remaining challenge is to understand how the additive cellulases improve substrate utilization efficiency but reduce the side-product glycerol concentration. Herein, we investigated a comprehensive responses pattern of cellulases-assisted ethanol production o ...
DOI:
10.1016/j.indcrop.2021.113653

10. Introducing hydroxyl groups as cellulose-binding sites into polymeric solid acids to improve their catalytic performance in hydrolyzing cellulose

Author:
Qiang Yang; Xuejun Pan
Source:
Carbohydrate polymers 2021 v.261 pp. 117895
ISSN:
0144-8617
Subject:
cellulases,   etc   ; biomass; catalytic activity; cellulose; glucose; hydrolysis; lignocellulose; polymerization; polymers; sulfonic acids; surface area;     Show all 11 Subjects
Abstract:
... Effective hydrolysis of cellulose to glucose is a crucial step to produce fuels and chemicals from lignocellulosic biomass. Solid acids are promising alternatives of cellulases and homogenous acids for hydrolyzing cellulose. In this study, porous polymeric solid acids bearing hydroxyl and sulfonic acid groups were fabricated for cellulose hydrolysis in water through the low-cost Friedel-Crafts “kn ...
DOI:
10.1016/j.carbpol.2021.117895

11. Comparative metagenomic discovery of the dynamic cellulose-degrading process from a synergistic cellulolytic microbiota

Author:
Ming Yang; Jingjing Zhao; Yue Yuan; Xiaoyi Chen; Fan Yang; Xianzhen Li
Source:
Cellulose 2021 v.28 no.4 pp. 2105-2123
ISSN:
0969-0239
Subject:
cellulases,   etc   ; Sporocytophaga; biodegradation; catabolism; cellobiose; cellulose; community structure; genes; hydrolysates; ion exchange chromatography; metagenomics; microorganisms;     Show all 12 Subjects
Abstract:
... To reveal the dynamic process of cellulose biodegradation and explore more potential cellulases, a microbiota (FPDM) with cellulose-degrading ability was cultivated, and different stages of filter paper degradation were compared. Ion chromatography and comparative metagenomic sequencing revealed that the diversity of FPDM enhanced as the hydrolysate length diversity increased. Sporocytophaga and C ...
DOI:
10.1007/s10570-020-03671-z

12. Fractionating process of lignocellulosic biomass for the enzymatic production of short chain cello-oligosaccharides

Author:
Patrícia F. Ávila; Rosana Goldbeck
Source:
Industrial crops and products 2022 v.178 pp. 114671
ISSN:
0926-6690
Subject:
cellulases,   etc   ; alkali treatment; biomass; cellulose; chlorites; delignification; esterases; glucose; hulls; lignocellulose; straw; sugarcane; temperature; wastes;     Show all 14 Subjects
Abstract:
... This study performed a prior delignification with sodium chlorite treatment on sugarcane straw and coffee husk waste, followed by alkaline treatment strategies; aiming at the increase cellulose recovery efficiency and the decrease of chemical consumption process. This study also explored an effective approach in the use of the cellulose recovery fraction for the enzymatic production of short chain ...
DOI:
10.1016/j.indcrop.2022.114671

13. Penicillium Ochrochloron RLS11 Secretome Containing Carbohydrate-Active Enzymes Improves Commercial Enzyme Mixtures During Sugarcane Straw Saccharification

Author:
Túlio Morgan; Daniel Luciano Falkoski; Murillo Peterlini Tavares; Mariana Bicalho Oliveira; Valéria Monteze Guimarães; Tiago Antônio de Oliveira Mendes
Source:
Applied biochemistry and biotechnology 2022 v.194 no.7 pp. 2946-2967
ISSN:
0273-2289
Subject:
cellulases,   etc   ; Basidiomycota; Penicillium; biomass; biotechnology; cell walls; fungi; genetic variation; proteomics; saccharification; straw; sugarcane; xylanases;     Show all 13 Subjects
Abstract:
... Filamentous fungi are prolific producers of carbohydrate-active enzymes (CAZymes) and important agents that carry out plant cell wall degradation in natural environments. The number of fungal species is frequently reported in the millions range, with a huge diversity and genetic variability, reflecting on a vast repertoire of CAZymes that these organisms can produce. In this study, we evaluated th ...
DOI:
10.1007/s12010-022-03898-5

14. Distinct Miscanthus lignocellulose improves fungus secreting cellulases and xylanases for consistently enhanced biomass saccharification of diverse bioenergy crops

Author:
Peng Liu; Ao Li; Youmei Wang; Qiuming Cai; Haizhong Yu; Yuqi Li; Hao Peng; Qian Li; Yanting Wang; Xiaoyang Wei; Ran Zhang; Yuanyuan Tu; Tao Xia; Liangcai Peng
Source:
Renewable energy 2021 v.174 pp. 799-809
ISSN:
0960-1481
Subject:
cellulases,   etc   ; Miscanthus; arabinose; bioenergy; biomass; cellulose; crystal structure; fuel production; fungi; hemicellulose; lignin; lignocellulose; saccharification; xylanases;     Show all 14 Subjects
Abstract:
... Bioenergy crops provide enormous renewable biomass resources convertible for biofuel production, but lignocellulose recalcitrance fundamentally determines its enzymatic saccharification at high cost and low efficiency. In this study, total 30 diverse Miscanthus lignocellulose substrates were incubated with T. reesei strain to secret lignocellulose-degradation enzymes, and their major wall polymers ...
DOI:
10.1016/j.renene.2021.04.107

15. Current understanding and optimization strategies for efficient lignin-enzyme interaction: A review

Author:
Mohan Li; Bo Jiang; Wenjuan Wu; Shufang Wu; Yiqin Yang; Junlong Song; Mehraj Ahmad; Yongcan Jin
Source:
International journal of biological macromolecules 2022 v.195 pp. 274-286
ISSN:
0141-8130
Subject:
cellulases,   etc   ; adsorption; bioethanol; cellulose; energy; enzymatic hydrolysis; feedstocks; lignin; lignocellulose; molecular weight; value added; water solubility;     Show all 12 Subjects
Abstract:
... From energy perspective, with abundant polysaccharides (45–85%), the renewable lignocellulosic is recognized as the 2nd generation feedstock for bioethanol and bio-based products production. Enzymatic hydrolysis is a critical pathway to yield fermentable monosaccharides from pretreated substrates of lignocellulose. Nevertheless, the lignin presence in lignocellulosic substrates leads to the low su ...
DOI:
10.1016/j.ijbiomac.2021.11.188

16. Diversity and distribution of thermophilic microorganisms and their applications in biotechnology

Author:
Safia Arbab; Hanif Ullah; Muhammad I. U. Khan; Muhammad N. K. Khattak; Jiyu Zhang; Ka Li; Inam Ul Hassan
Source:
Journal of basic microbiology 2022 v.62 no.2 pp. 95-108
ISSN:
0233-111X
Subject:
cellulases,   etc   ; Archaea; DNA; amylases; biotechnology; carboxylic ester hydrolases; ecosystems; humans; industry; proteinases; pullulanase; thermal stability; xylanases;     Show all 13 Subjects
Abstract:
... Hot springs ecosystem is the most ancient continuously inhabited ecosystem on earth which harbors diverse thermophilic bacteria and archaea distributed worldwide. Life in extreme environments is very challenging so there is a great potential biological dark matter and their adaptation to harsh environments eventually producing thermostable enzymes which are very vital for the welfare of mankind. T ...
DOI:
10.1002/jobm.202100529

17. Growth and phycocyanin production with Galdieria sulphuraria UTEX 2919 using xylose, glucose, and corn stover hydrolysates under heterotrophy and mixotrophy

Author:
Francisco Vera-López Portillo; Estefanía Sierra-Ibarra; Rosario Vera-Estrella; Sergio Revah; Octavio T. Ramírez; Luis Caspeta; Alfredo Martinez
Source:
Algal research 2022 v.65 pp. 102752
ISSN:
2211-9264
Subject:
cellulases,   etc   ; acetates; biomass production; corn stover; extremophiles; glucose; microalgae; phycocyanin; research; specific growth rate; syrups; xylose;     Show all 12 Subjects
Abstract:
... The production of cell mass and phycocyanin (PC) was evaluated with the extremophile microalgae Galdieria sulphuraria 2919, under mixotrophic and heterotrophic conditions, in media supplemented with glucose, xylose, or a mixture of both sugars, and hemicellulosic and cellulosic hydrolysates from corn stover. Compared to xylose, cultivations with glucose under heterotrophic conditions showed 51%, 4 ...
DOI:
10.1016/j.algal.2022.102752

18. Comparative analysis of two recombinant LPMOs from Aspergillus fumigatus and their effects on sugarcane bagasse saccharification

Author:
Josman Velasco; Vanessa de Oliveira Arnoldi Pellegrini; Ana Gabriela Veiga Sepulchro; Marco Antonio Seiki Kadowaki; Melissa Cristina Espirito Santo; Igor Polikarpov; Fernando Segato
Source:
Enzyme and microbial technology 2021 v.144 pp. 109746
ISSN:
0141-0229
Subject:
cellulases,   etc   ; Aspergillus fumigatus; anion exchange chromatography; biomass; cellulose; heat tolerance; hydrolysis; lignin; lignocellulose; oxidation; saccharification; sugarcane bagasse;     Show all 12 Subjects
Abstract:
... Lytic polysaccharide monooxygenases (LPMOs) have been introduced into industrial cocktails used for biomass saccharification due to their capacity to boost enzymatic conversion of recalcitrant cellulose. The genome of the thermotolerant ascomycete Aspergillus fumigatus encodes 7 genes for LPMOs that belong to auxiliary activity family 9 (AA9). Here, we cloned, successfully expressed and performed ...
DOI:
10.1016/j.enzmictec.2021.109746

19. Hybrid Genome Assembly for Predicting Functional Potential of a Novel Streptomyces Strain as Plant Biomass Valorisation Agent

Author:
Hitesh Tikariha; Shruti Pavagadhi; Sevugan Mayalagu; Miko Chin Hong Poh; Sanjay Swarup
Source:
Indian journal of microbiology 2021 v.61 no.3 pp. 283-290
ISSN:
0046-8991
Subject:
cellulases,   etc   ; Streptomyces; bioremediation; biosynthesis; chitin; farms; genome assembly; hybrids; lignin; lignocellulose; lignocellulosic wastes; microbiology; nanopores; phytomass; prediction; rhizosphere;     Show all 16 Subjects
Abstract:
... Environmental bioremediation relies heavily on the realized potential of efficient bioremediation agents or microbial strains of interest. Identifying suitable microbial agents for plant biomass waste valorization requires (i) high-quality genome assemblies to predict the full metabolic and functional potential, (ii) accurate mapping of lignocellulose metabolizing enzymes. However, fragmented natu ...
DOI:
10.1007/s12088-021-00935-5

20. Integrating bio-oil and carbohydrate valorization on the fractionation of sugarcane bagasse via Organosolv process using Mo2C-based catalysts

Author:
Gleicielle Tozzi Wurzler; Victor Teixeira da Silva; Débora de Almeida Azevedo; Ayla Sant’ Ana da Silva; Fábio Bellot Noronha
Source:
Fuel processing technology 2022 v.230 pp. 107208
ISSN:
0378-3820
Subject:
cellulases,   etc   ; activated carbon; biofuels; catalysts; delignification; depolymerization; fractionation; furfural; glucose; glycols; hemicellulose; isopropyl alcohol; molybdenum; sugarcane bagasse; xylose;     Show all 15 Subjects
Abstract:
... This work studied the fractionation of sugarcane bagasse via Organosolv treatment using isopropanol/water in the presence of Raney-Ni and molybdenum carbide catalysts (Bulk Mo₂C and Mo₂C supported on activated carbon (AC) or Al₂O₃). The degree of delignification, the bio-oil and solid residue composition depended on the type of catalyst. A partial extraction of hemicellulose occurred followed by d ...
DOI:
10.1016/j.fuproc.2022.107208