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... Recent advances in the sequencing and assembly of plant genomes have allowed the generation of genomes with increasing contiguity and sequence accuracy. Chromosome level genome assemblies using sequence contigs generated from long read sequencing have involved the use of proximity analysis (Hi‐C) or traditional genetic maps to guide the placement of sequence contigs within chromosomes. The develop ...
chromosomemapping, etc ; Panax notoginseng; genes; nitrogen; people; phylogeny; China; Show all 7 Subjects
Abstract:
... Panax notoginseng (Burkill) F. H. Chen ex C. H. increasingly entered into people's daily life, but its quality has been greatly affected in recent years. Panax notoginseng is one of the representatives of the ginseng plant of Araliaceae, which is an important and precious local medicinal material in China. Basic helix–loop–helix transcription factors play an irreplaceable position in the life of p ...
chromosomemapping, etc ; barley; genetic recombination; genomics; genotyping by sequencing; Show all 5 Subjects
Abstract:
... As genotyping-by-sequencing (GBS) is widely used in barley genetic studies, the translation of the physical position of GBS-derived SNPs into accurate genetic positions has become relevant. The main aim of this study was to develop a high-resolution consensus linkage map based on GBS-derived SNPs. The construction of this integrated map involved 11 bi-parental populations composed of 3743 segregat ...
chromosomemapping, etc ; Fagopyrum esculentum; agronomy; buckwheat; genes; phenotype; pseudocereals; transcriptome; Ukraine; Show all 9 Subjects
Abstract:
... Common buckwheat (Fagopyrum esculentum M.) is an important pseudo-cereal crop and contains an abundance of nutrients and bioactive compounds. However, the yield of buckwheat is low compared to that of other major crops. QTL mapping and candidate gene screening of grain size are very important for increasing production in common buckwheat through molecular breeding in the future. In the present stu ...
Chie Sawada; Carol Moreau; Gabriel H. J. Robinson; Burkhard Steuernagel; Luzie U. Wingen; Jitender Cheema; Ellen Sizer-Coverdale; David Lloyd; Claire Domoney; Noel Ellis
chromosomemapping, etc ; Pisum sativum; cultivars; dissection; inbred lines; peas; population structure; Show all 7 Subjects
Abstract:
... Biparental recombinant inbred line (RIL) populations are sets of genetically stable lines and have a simple population structure that facilitates the dissection of the genetics of interesting traits. On the other hand, populations derived from multiparent intercrosses combine both greater diversity and higher numbers of recombination events than RILs. Here, we describe a simple population structur ...
chromosomemapping, etc ; genome; genomics; genotyping by sequencing; single nucleotide polymorphism; soybeans; Show all 6 Subjects
Abstract:
... Genetic linkage maps are used to localize markers on the genome based on the recombination frequency. Most often, these maps are based on the segregation observed within a single biparental population of limited size (n < 300) where relatively few recombination events are sampled and in which some genomic regions are monomorphic because both parents carry the same alleles. Together, these two limi ...
chromosomemapping, etc ; Aphelinus; Aphidoidea; biological control; genes; host specificity; parasitoids; quantitative traits; Show all 8 Subjects
Abstract:
... Safe, effective biological-control introductions against invasive pests depend on narrowly host-specific natural enemies with the ability to adapt to a changing environment. As part of a project on the genetic architectures of these traits, we assembled and annotated the genomes of two aphid parasitoids, Aphelinus atriplicis and Aphelinus certus. We report here several assemblies of A. atriplicis ...
chromosomemapping, etc ; DNA; backcrossing; color; cultivars; genomics; introgression; leaves; peaches; phenotype; progeny; Show all 11 Subjects
Abstract:
... Based on detailed maps, DNA sequences and phenotypic data, there is a great deal of information on the genetics and genomics of ‘Earlygold’, a historical peach cultivar from the US. The F₂ between ‘Texas’ almond and ‘Earlygold’ peach (T × E) was used to construct the first saturated peach linkage map that later became the reference map for the Prunus genus. This population and the first backcross ...
chromosomemapping, etc ; Takifugu; chromosomes; economic valuation; genes; genetic recombination; genome assembly; genomics; Show all 8 Subjects
Abstract:
... Takifugu genus has been brought to the fore in scientific and practical research due to its compact genome, explosive speciation progress and economic value. Here we updated the chromosome-level genome of Takifugu bimaculatus by an ultra-high-density linkage map, a classic and accurate way of chromosome assembly. The map constituted a robust assembly frame, with 92.2% (372.77 Mb) of the draft geno ...
chromosomemapping, etc ; DNA fragmentation; circular DNA; genomics; humans; meiosis; microscopy; somatic cells; spermatozoa; Show all 9 Subjects
Abstract:
... Extrachromosomal circular DNA (eccDNA) is common in somatic tissue, but its existence and effects in the human germline are unexplored. We used microscopy, long-read DNA sequencing, and new analytic methods to document thousands of eccDNAs from human sperm. EccDNAs derived from all genomic regions and mostly contained a single DNA fragment, although some consisted of multiple fragments. The genera ...
chromosomemapping, etc ; Acipenser baerii; conservation genetics; fish roe; gene frequency; heterozygosity; water pollution; Show all 7 Subjects
Abstract:
... Acipenser baerii is one of the most important sources of caviar production. However, the population of the A. baerii is constantly being reduced in recent years due to dam construction, overfishing, and water pollution. Insufficient molecular markers have limited the effective conservation and management of this species; In the present study, we reported the isolation and characterization of 34 SN ...
chromosomemapping, etc ; cost effectiveness; cotton; genomics; genotype; genotyping; liquids; single nucleotide polymorphism arrays; Show all 8 Subjects
Abstract:
... Genotyping platforms provide robust support for genomics, genetics, and molecular breeding. Given the economic importance of cotton, it is urgent to develop a cotton genotyping platform that features high throughput, precision, efficiency, and cost-effectiveness. Here we reported the development of a liquid SNP chip named “ZJU CottonSNP40K” through genotyping by target sequencing, along with its u ...
chromosomemapping, etc ; Solanum melongena; delphinidin; eggplants; fruits; genes; metabolism; molecular biology; myrtillin; tomatoes; Show all 10 Subjects
Abstract:
... BACKGROUND: The appearance quality of the eggplant (Solanum melongena L.) fruit is an important trait that influences its commercial value. It is known that quality traits such as anthocyanin composition and fruit surface pattern are categorical and are inherited simply. However, research examples of gene mapping for the composition (anthocyanin accumulation profile) and the surface pattern in egg ...
chromosomemapping, etc ; alleles; cultivars; genotyping; inbred lines; marker-assisted selection; phenotype; seeds; wheat; Show all 9 Subjects
Abstract:
... BACKGROUND: Kernel number per spike (KNS) and spike length (SL) are important spike-related traits in wheat variety improvement. Discovering genetic loci controlling these traits is necessary to elucidate the genetic basis of wheat yield traits and is very important for marker-assisted selection breeding. RESULTS: In the present study, we used a recombinant inbred line population with 248 lines de ...
chromosomemapping, etc ; DNA; Litopenaeus vannamei; aquaculture; body weight; genomics; genotyping; quantitative traits; salinity; shrimp; Show all 10 Subjects
Abstract:
... Low-salinity tolerance and growth are crucial economic traits of shrimp in areas with low-salinity water. In the current study, an F1 full-sib family of Pacific white shrimp (Litopenaeus vannamei) was obtained by the “semidirectional cross” method, and 2 parents and 146 progenies were genotyped using double-digest restriction site-associated DNA sequencing technology (ddRAD-Seq). A total of 3335 h ...
Hafiz Muhammad Khalid Abbas; Yang Yang Zhou; He Xun Huang; Muhammad Tahir ul Qamar; Man Man Wang; Sen Lu; Congping Xu; Zhanshu Liu; Ting Quan Wu; Yu Juan Zhong
chromosomemapping, etc ; females; genes; loci; males; marker-assisted selection; pumpkins; quantitative traits; sequence analysis; Show all 9 Subjects
Abstract:
... Finding the quantitative trait loci (QTLs) and uncovering the genes controlling early flowering in pumpkin are the basic needs to select appropriate varieties for cultivation. Two QTLs related to first female flowering node (FFFN) and four QTLs related to first male flowering node (FMFN) were detected based on high‐density genetic map of pumpkin. Based on annotation information, two candidate gene ...
chromosomemapping, etc ; Setaria italica; dissection; genomics; models; panicles; phenotypic variation; plant height; quantitative traits; Show all 9 Subjects
Abstract:
... Foxtail millet (Setaria italica) is an ideal model of genetic system for functional genomics of the Panicoideae crop. Identification of QTL responsible for morpho-agronomic and yield-related traits facilitates dissection of genetic control and breeding in cereal crops. Here, based on a Yugu1 × Longgu7 RIL population and genome-wide resequencing data, an updated linkage map harboring 2297 bin and 7 ...
chromosomemapping, etc ; Benincasa hispida; chromosomes; color; fruits; genotyping; horticultural crops; phenotypic variation; quantitative traits; Show all 9 Subjects
Abstract:
... Wax gourd (Benincasa hispida [Thunb] Cogn.) is an important horticultural crop in the Cucurbitaceae family that has high nutritive and medicinal value. We analysed quantitative trait loci (QTLs) related to the fruit-related traits of wax gourd by constructing a high-density genetic map through whole-genome resequencing. We obtained an F₂ population of 195 individuals by crossing two inbred wax gou ...
chromosomemapping, etc ; Arabidopsis; fruits; genomics; genotyping; melons; rice; seed size; sequence analysis; vegetable crops; Show all 10 Subjects
Abstract:
... Melon is a popular fruit vegetable crop worldwide with diverse morphological variation. We report a high-density genetic map of melon and nine major QTLs with physical region ranging from 43.47 kb to 1.89 Mb. Importantly, two seed-related trait QTLs were repeatedly detected in two environments, and the mapping region was narrowed to 522 kb according to a regional linkage analysis. A total of 40 an ...
chromosomemapping, etc ; Arachis hypogaea; DNA; Ralstonia solanacearum; bacterial wilt; disease resistance; germplasm; peanuts; China; Show all 9 Subjects
Abstract:
... KEY MESSAGE: A major QTL, qBWA12, was fine mapped to a 216.68 kb physical region, and A12.4097252 was identified as a useful KASP marker for breeding peanut varieties resistant to bacterial wilt. Bacterial wilt, caused by Ralstonia solanacearum, is a major disease detrimental to peanut production in China. Breeding disease-resistant peanut varieties is the most economical and effective way to prev ...