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1. Isochoric conditions enable high subfreezing temperature pancreatic islet preservation without osmotic cryoprotective agents

Author:
Powell-Palm, Matthew J.; Zhang, Yanfang; Aruda, Justin; Rubinsky, Boris
Source:
Cryobiology 2019 v.86 pp. 130-133
ISSN:
0011-2240
Subject:
cryopreservation,   etc   ; cryoprotectants; islets of Langerhans; temperature; thermodynamics;     Show all 5 Subjects
Abstract:
... While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day preservation at high subfreezing temperatures in a ...
DOI:
10.1016/j.cryobiol.2019.01.003

2. Cryopreservation and characterization of canine preantral follicles

Author:
Jivago, J.L.P.R.; Paulini, F.; Silva, R.C.; Araujo, M.S.; Marinho, A.P.S.; Lucci, C.M.
Source:
Cryobiology 2018 v.81 pp. 34-42
ISSN:
0011-2240
Subject:
cryopreservation,   etc   ; bitches; freezing; ultrastructure; vitrification;     Show all 5 Subjects
Abstract:
... The aim of this study was to define the population, morphological and ultrastructural characteristics of bitch preantral follicles (PAFs) and to compare the effects on the morphology of PAF of two cryopreservation techniques - slow freezing (SF) and vitrification (V) - of bitches' ovarian tissue. The average population (number per ovary) of PAFs was 48,541 ± 18,366, where 94.25% were primordial (4 ...
DOI:
10.1016/j.cryobiol.2018.02.013

3. Long-term maintenance of 23 strains of freshwater microalgae on solid microbiological culture media: A preliminary study

Author:
Yee, Willy; Tang, Shirley Gee Hoon; Phua, Pamela Szu Phin; Megawarnan, Himayawathy
Source:
Algal research 2019 v.41 pp. 101516
ISSN:
2211-9264
Subject:
cryopreservation,   etc   ; agar; coconut milk; culture media; glucose; microalgae; sucrose;     Show all 7 Subjects
Abstract:
... In this work, the ability of 23 strains of freshwater microalgae to be maintained on 10 different solid culture media was evaluated. Depending on the type of media, it was found that all strains of microalgae could be recovered after 19–24 months of culture in the dark. Bold's basal medium (BBM), BBM + 5% (v/v) coconut milk (CMA) and BBM + 5% (w/v) powdered skim milk (SMA) solidified with 1.5% (w/ ...
DOI:
10.1016/j.algal.2019.101516

4. Cryopreservation of cell line Paesun by a rapid cooling

Author:
Song, Jong Kui; Hui, Yu Lan
Source:
Cryobiology 2019 v.87 pp. 115-116
ISSN:
0011-2240
Subject:
cryopreservation,   etc   ; cell lines; cooling; liquids; nitrogen; staining; thawing; vapors; viruses;     Show all 9 Subjects
Abstract:
... The purpose of the present study was to clarify the possibility of a rapid cryopreservation for cell line Paesun by cooling in the range of 30–40 °C/min to vapor phase of −120 ∼-140 °C before immersion into liquid phase of liquid nitrogen using 10% Me2SO. After thawing, these cells were examined with assaying viability by trypan blue exclusion staining and survival by cloning in monolayer; the per ...
DOI:
10.1016/j.cryobiol.2019.01.017

5. Cryopreservation of shoot tips of recalcitrant and tropical species: Advances and strategies

Author:
Normah, M.N.; Sulong, Norafarain; Reed, Barbara M.
Source:
Cryobiology 2019 v.87 pp. 1-14
ISSN:
0011-2240
Subject:
cryopreservation,   etc   ; germplasm conservation; new species; recalcitrant seeds; shoot tips; temperature;     Show all 6 Subjects
Abstract:
... There is a pressing need for practical and successful conservation efforts to establish long-term germplasm collections of recalcitrant and tropical species, given the challenge and threat that these plants are facing. Cryopreservation is the only way of conserving some of these species, especially those with temperature or desiccation sensitive (recalcitrant) seeds. This review covers reports on ...
DOI:
10.1016/j.cryobiol.2019.01.008

6. Post‐thawing effects of three cryopreservation diluents on Rusa deer (Rusa timorensis) spermatozoa

Author:
Monaco, Davide; Zappia, Eduardo; Apichaya, Sudsukh; Lacalandra, Giovanni M.; Thongtip, Nikorn
Source:
Reproduction in domestic animals 2019 v.54 no.3 pp. 635-638
ISSN:
0936-6768
Subject:
cryopreservation,   etc   ; Rusa timorensis; acrosome; artificial insemination; semen; thermal stress; viability;     Show all 7 Subjects
Abstract:
... The aim of this study was to evaluate home‐made and commercial extenders for the cryopreservation of Rusa deer semen. After collection by electroejaculation, six ejaculates were diluted and frozen in TES‐based, Tris‐based and Triladyl® extenders. Subjective motility, viability, morphology, acrosome integrity and membrane functionality were assessed post‐thawing and after 1‐hr incubation at 37°C (T ...
DOI:
10.1111/rda.13380

7. The comparison of two different extenders for the improvement of large‐scale sperm cryopreservation in common carp (Cyprinus carpio)

Author:
Várkonyi, Levente; Bokor, Zoltán; Molnár, József; Fodor, Ferenc; Szári, Zsolt; Ferincz, Árpád; Staszny, Ádám; Láng, Levente Zete; Csorbai, Balázs; Urbányi, Béla; Bernáth, Gergely
Source:
Reproduction in domestic animals 2019 v.54 no.3 pp. 639-645
ISSN:
0936-6768
Subject:
cryopreservation,   etc   ; Cyprinus carpio; agglutination; carp; freezers; polystyrenes; spermatozoa; straw; thawing;     Show all 9 Subjects
Abstract:
... In our study, a traditionally used (Grayling, already used in cyprinid species) and a newly tested (Pike) extender was tested to avoid sperm agglutination phenomenon following thawing during carp sperm cryopreservation. A large‐scale (elevated volume of sperm) freezing method in a controlled‐rate freezer using 5 ml straw and 10 ml cryotube was also systematically established. In all experiments, t ...
DOI:
10.1111/rda.13383

8. The role of progesterone elevation in IVF

Author:
Drakopoulos, Panagiotis; Racca, Annalisa; Errázuriz, Joaquín; De Vos, Michel; Tournaye, Herman; Blockeel, Christophe; Pluchino, Nicola; Santos-Ribeiro, Samuel
Source:
Reproductive Biology 2019 v.19 no.1 pp. 1-5
ISSN:
1642-431X
Subject:
cryopreservation,   etc   ; endometrium; in vitro fertilization; pregnancy outcome; progesterone;     Show all 5 Subjects
Abstract:
... Elevation of progesterone during the late follicular phase of stimulated in-vitro fertilization cycles is a frequent event, which negatively impacts the outcome. Over the years evidence has demonstrated a direct relationship between late-follicular elevated progesterone and endometrial receptivity. In this regard, elective cryopreservation of all good quality embryos and transfer in a subsequent f ...
DOI:
10.1016/j.repbio.2019.02.003

9. Development of the droplet-vitrification cryopreservation technique as a viable alternative to the dormant bud technique for recalcitrant pears

Author:
Guyader, A.; Grapin, A.; Le Bras, C.; Laurens, F.
Source:
Acta horticulturae 2019 no.1234 pp. 219-224
ISSN:
0567-7572
Subject:
cryopreservation,   etc   ; Cydonia oblonga; Malus; Pyrus; cultivars; fruits; nitrogen; pears;     Show all 8 Subjects
Abstract:
... The French INRA Pip Fruit Biological Resource Center is in charge of the maintenance, management, characterization and promotion of the traditional and scientific genetic resources of apple, pear, quince and related species. In 2010, the first cryopreservation trials were performed on apple and pear using the dormant bud technique with the main aim of cryopreserving a major part of the INRA Malus ...
DOI:
10.17660/ActaHortic.2019.1234.29

10. Cryopreservation of donkey embryos by the cryotop method: Effect of developmental stage, embryo quality, diameter and age of embryos

Author:
Bottrel, M.; Ortiz, I.; Pereira, B.; Díaz-Jiménez, M.; Hidalgo, M.; Consuegra, C.; Morató, R.; Mogas, T.; Dorado, J.
Source:
Theriogenology 2019 v.125 pp. 242-248
ISSN:
0093-691X
Subject:
cryopreservation,   etc   ; asses; breeds; dyes; propidium; survival rate; viability; vitrification;     Show all 8 Subjects
Abstract:
... Cryopreservation of embryos has the potential to become a valuable tool for the conservation of endangered donkey breeds. However, there are several factors that can affect cryosurvival of embryos. This study evaluates the effectiveness of the Cryotop method to vitrify donkey embryos and factors affecting the survival of vitrified-warmed embryos. Day 6–8 embryos were measured and morphologically e ...
DOI:
10.1016/j.theriogenology.2018.11.011

11. Cryopreservation of mammalian embryos: Derivation of a method

Author:
Leibo, S.P.; Sztein, Jorge M.
Source:
Cryobiology 2019 v.86 pp. 1-9
ISSN:
0011-2240
Subject:
cryopreservation,   etc   ; cattle; embryo (animal); freezing; humans; medicine; mice; mothers; progeny;     Show all 9 Subjects
Abstract:
... In 1972, a procedure was derived to cryopreserve mouse embryos. Over the past four decades, this procedure has been adapted to freeze embryos of more than twenty-five mammalian species. Cryopreservation of embryos has become a routine procedure in both veterinary and human medicine, having been used to freeze millions of embryos of mice and cattle, and many hundreds of thousands of human embryos. ...
DOI:
10.1016/j.cryobiol.2019.01.007

12. Are cells from a snowman realistic? Cryopreserved tissues as a source for single-cell RNA-sequencing experiments

Author:
Vieira Braga, Felipe A.; Teichmann, Sarah A.; Stubbington, Michael J. T.
Source:
Genome biology 2017 v.18 no.1 pp. 54
ISSN:
1474-760X
Subject:
cryopreservation,   etc   ; genome; tissues;     Show all 3 Subjects
Abstract:
... A recently published study in Genome Biology shows that cells isolated from cryopreserved tissues are a reliable source of genetic material for single-cell RNA-sequencing experiments.Please see related Method article: http://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1171-9 ...
DOI:
10.1186/s13059-017-1192-4
PubMed:
28340618
PubMed Central:
PMC5364640

13. Autologous endothelialized vein allografts in coronary artery bypass surgery – Long term results

Author:
Herrmann, Florian Ernst Martin; Lamm, Peter; Wellmann, Petra; Milz, Stefan; Hagl, Christian; Juchem, Gerd
Source:
Biomaterials 2019 v.212 pp. 87-97
ISSN:
0142-9612
Subject:
cryopreservation,   etc   ; allografting; angiography; collagen; coronary vessels; death; endothelial cells; immunohistochemistry; monocytes; necropsy; patients;     Show all 11 Subjects
Abstract:
... Lack of autologous graft material restricts the ability to treat patients requiring coronary artery bypass surgery (CABG). An off the shelf tissue engineered small diameter vascular graft is the holy grail of cardiovascular surgery.Allograft saphenous veins were harvested from organ donors, cryopreserved, deendothelialized and then seeded with autologous endothelial cells prior to implantation dur ...
DOI:
10.1016/j.biomaterials.2019.05.019

14. Renaming of ‘Candidatus Methylocucumis oryzae’ as Methylocucumis oryzae gen. nov., sp. nov., a novel Type I methanotroph isolated from India

Author:
Pandit, Pranitha S.; Rahalkar, Monali C.
Source:
Antonie van Leeuwenhoek 2019 v.112 no.6 pp. 955-959
ISSN:
0003-6072
Subject:
cryopreservation,   etc   ; Methylocucumis; calcium; dimethyl sulfoxide; methanotrophs; new genus; new species; paddies; India; Japan; Korean Peninsula;     Show all 11 Subjects
Abstract:
... Candidatus Methylocucumis (Mcu.) oryzae, strain Sn10-6, a Gram-negative, pale pink pigmented, motile, large Type I methanotroph (3–6 µm × 1.5–2 µm) was recently isolated from an Indian rice field. Due to the difficulties in preservation of the strain it could not be deposited and preserved in two recognised culture collections with public access located in two different countries. Hence, Sn10-6 wa ...
DOI:
10.1007/s10482-018-01218-8

15. Influence of Different Combinations of Permeable and Nonpermeable Cryoprotectants on the Freezing Capacity of Equine Sperm

Author:
Vafaei, Farhad; Kohram, Hamid; Zareh-Shahne, Ahmad; Ahmad, Ejaz; Seifi-Jamadi, Afshin
Source:
Journal of equine veterinary science 2019 v.75 pp. 69-73
ISSN:
0737-0806
Subject:
cryopreservation,   etc   ; cryoprotectants; freezing; glycerol; lipid peroxidation; plasma membrane; spermatozoa; stallions; sucrose; synergism; trehalose; viability;     Show all 12 Subjects
Abstract:
... This study was aimed to evaluate the effect of permeable cryoprotectants in combination with trehalose or sucrose on the freezing capacity of stallion sperm. For this purpose, the ejaculates (n = 24) were collected from four healthy mature Turkmen stallions. The ejaculates were pooled and diluted with one of the extenders containing a combination of 5% of permeating (dimethylacetamide [DMA]; dimet ...
DOI:
10.1016/j.jevs.2019.01.014

16. Numerical analysis of an enhanced cooling rate cryopreservation process in a biological tissue

Author:
Sukumar, S.; Kar, S.P.
Source:
Journal of thermal biology 2019 v.81 pp. 146-153
ISSN:
0306-4565
Subject:
cryopreservation,   etc   ; algorithms; blood; differential equation; freezing; functional properties; heat; mathematical models; temperature; tissues;     Show all 10 Subjects
Abstract:
... Cryopreservation is the method of preservation of biological tissues for future references without causing significant damages to their physical and functional properties. This can be done by exposing them to very low cryogenic temperature that involves a greater heat removal rate. A two dimensional numerical model is developed to study the temperature distribution, cooling rate attained and movem ...
DOI:
10.1016/j.jtherbio.2019.03.001

17. Post-thaw Addition of Caffeine and/or Pentoxifylline Affect Differently Motility of Horse and Donkey-Cryopreserved Spermatozoa

Author:
Rota, Alessandra; Sabatini, Chiara; Przybył, Anna; Ciaramelli, Alessia; Panzani, Duccio; Camillo, Francesco
Source:
Journal of equine veterinary science 2019 v.75 pp. 41-47
ISSN:
0737-0806
Subject:
cryopreservation,   etc   ; additives; asses; caffeine; in vitro fertilization; milk; pentoxifylline; pregnancy rate; semen; sperm motility; spermatozoa; stallions;     Show all 12 Subjects
Abstract:
... To increase sperm motility, several molecules have been tested in mammals. Methylxanthines have shown effects on sperm motility, capacitation, and on in vitro fertilization processes. The aim of the study was to evaluate if the post-thaw addition of caffeine and/or pentoxifylline changes motility parameters of cryopreserved stallion and donkey spermatozoa. Straws derived from 14 horses and 7 donke ...
DOI:
10.1016/j.jevs.2019.01.003

18. Royal jelly (1%) were successfully used for drone semen cryopreservation successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders

Author:
Alcay, Selim; Cakmak, Selvinar; Cakmak, Ibrahim; Mulkpinar, Emine; Gokce, Elif; Ustuner, Burcu; Sen, Hasan; Nur, Zekariya
Source:
Cryobiology 2019
ISSN:
0011-2240
Subject:
cryopreservation,   etc   ; plasma membrane; royal jelly; semen; sexual maturity; sperm motility; spermatozoa; thawing;     Show all 8 Subjects
Abstract:
... The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thawing quality of drone sperm. Semen samples were collected from sexually mature drones. Pooled semen was diluted with extender without RJ (control) or supplemented with different concentrations of RJ (1, 2, 4 or 8%). Sperm motility, plasma membrane functional integrity, and a ...
DOI:
10.1016/j.cryobiol.2019.03.005

19. Antioxidants and glycine can improve the developmental competence of vitrified/warmed ovine immature oocytes

Author:
Ahmadi, Ebrahim; Shirazi, Abolfazl; Shams‐Esfandabadi, Naser; Nazari, Hassan
Source:
Reproduction in domestic animals 2019 v.54 no.3 pp. 595-603
ISSN:
0936-6768
Subject:
cryopreservation,   etc   ; antioxidants; apoptosis; ascorbic acid; blastocyst; cysteine; germinal vesicle; metaphase; oxidative stress; sheep; survival rate; vitrification;     Show all 12 Subjects
Abstract:
... Despite the numerous potential applications of oocyte cryopreservation, the poor success rate has limited its practical applications. In livestock, particularly in ovine, the oocytes have low developmental competence following vitrification/warming process. Considering the occurrence of osmotic and oxidative stresses during the vitrification/warming process, the application of antioxidants and osm ...
DOI:
10.1111/rda.13402

20. Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender

Author:
Igbokwe, Abigail A.; Iyasere, Oluwaseun S.; Sobayo, Richard A.; Iyasere, Seyifunmi; Animashaun, Rukayat I.; Balogun, Fatimoh A.; Aganran, Zainab O.; Fasola, Morakinyo O.; Adedokun, Afeez D.; Lakehinde, Olawale A.; Lasisi, Sodiq O.; Suleiman, Muhammad R.; Iyiola, Jamiu D.; Daramola, James O.
Source:
Reproduction in domestic animals 2019 v.54 no.3 pp. 551-559
ISSN:
0936-6768
Subject:
cryopreservation,   etc   ; acrosin; acrosome; bucks; freezing; goats; malondialdehyde; oxidative stress; semen; tiger nut milk;     Show all 10 Subjects
Abstract:
... Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk (TNM) extender was examined in this study. Pooled semen samples obtained from West African Dwarf (WAD) goat bucks were diluted with Tris‐based extenders containing different levels of TNM (0, 5, 10, 15 and 20 ml/100 ml extender). The dil ...
DOI:
10.1111/rda.13393