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cryopreservation, etc ; Cyprinus carpio; carp; cryoprotectants; eggs; embryogenesis; sperm concentration; sperm motility; spermatozoa; Show all 9 Subjects
Abstract:
... To avoid mechanical compression of spermatozoa during the cryopreservation, a high dilution ratio with the cryoprotective medium is applied. Due to this, the sample volume increases, which entails increased cryobank capacity. Moreover, the high volume of cryomedias does not allow to fertilize of large volumes of eggs in practical artificial reproduction of common carp aquaculture. The current stud ...
... Ice recrystallization inhibitors are novel cryoprotective agents that can reduce the freezing damage of cells, tissues, and organs in cryopreservation. To date, potent ice recrystallization inhibition (IRI) activity has been found on antifreeze (glyco)proteins, polymers, nanomaterials, and a limited number of chemically synthesized small molecules. This paper reports a relatively potent IRI activi ...
cryopreservation, etc ; cattle; markets; pregnancy rate; vitrification; Show all 5 Subjects
Abstract:
... The expansion of the use of in vitro production techniques has revolutionized the bovine embryo market. In the last decade, we have seen the number of in vitro produced (IVP) embryos surpass the number of in vivo-derived (IVD) embryos obtained worldwide. Concomitantly, other biotechnologies were also improved, following the global trend. Embryo cryopreservation has received special attention, as i ...
cryopreservation, etc ; cryoprotectants; crystallization; ice; light microscopes; Show all 5 Subjects
Abstract:
... Cyclodextrin-based cryoprotectants were developed. α-TMCD, which can be easily put into large-scale production, showed enhanced cell viabilities of 19.97 ± 0.78%, 13.93 ± 4.46% and 19.10 ± 0.95% against GES-1, hucMSCs and A549 cells. Moreover, the viable cells observed by light microscope imaging showed that the enhanced hucMSC cell number percentage of α-TMCD was 103.2%. An α-TMCD-DMSO-based CPA ...
cryopreservation, etc ; cryoprotectants; cytotoxicity; ice; ice nucleation; medicine; Show all 6 Subjects
Abstract:
... Cryopreservation of tissues and organs can bring transformative changes to medicine and medical science. In the past decades, limited progress has been achieved, although cryopreservation of tissues and organs has long been intensively pursued. One key reason is that the cryoprotective agents (CPAs) currently used for cell cryopreservation cannot effectively preserve tissues and organs because of ...
cryopreservation, etc ; blastocyst; eggs; embryo transfer; humans; oocytes; Show all 6 Subjects
Abstract:
... Centrally located cytoplasmic granulation (central granulation) is a common cytoplasmic dysmorphism in human oocytes retrieved after controlled ovarian hyperstimulation (COH). In order to achieve a better understanding of its formation and effects on clinical outcomes, we retrospectively analyzed 422 ICSI treatment cycles. Three groups of patients were classified according to the ratio of central ...
cryopreservation, etc ; animals; cell structures; cold storage; tissues; Show all 5 Subjects
Abstract:
... Cryopreservation is a fundamental procedure to preserve the structure and function of cells and tissues by storing them at low temperatures for long periods [...] ...
cryopreservation, etc ; Dipterocarpaceae; Pittosporaceae; Rhizophoraceae; herbaceous plants; pollen; Show all 6 Subjects
Abstract:
... Cryopreservation is increasingly important as a conservation tool, particularly for threatened exceptional species. The goal of this study was to investigate the current knowledge of plant cryopreservation through a search of the literature in Web of Science and align that with the 775 species currently identified on the Working List of Exceptional Plants. While there is a good foundation in plant ...
cryopreservation, etc ; Gibbs free energy; evolution; ice; metrology; temperature; Show all 6 Subjects
Abstract:
... Phase diagrams are integral to the application and interpretation of materials thermodynamics, and none is more ubiquitous than the common temperature–pressure diagram of water and its many icy phases. Inspired by recent advances in isochoric thermodynamics, we here employ a simple convex hull approach to efficiently calculate an updated temperature–volume phase diagram for water and five of its s ...
cryopreservation, etc ; fluorescence microscopy; linoleic acid; mice; viability; vitrification; Show all 6 Subjects
Abstract:
... The study examines the effect of in vitro cultivation of mouse embryos for 48 h with 200 µM linoleic acid (LA) on their viability after vitrification. Fluorescence microscopy of DAPI-stained embryos showed increased fragmentation of nuclei in case of exposure to LA without cryopreservation and a significant decrease in the number of cells in vitrified embryos 24 h after their thawing. Thus, in vit ...
cryopreservation, etc ; adhesion; digestion; dissociation; mesothelioma; neoplasm cells; phenotype; Show all 7 Subjects
Abstract:
... Current methodologies for the expansion of primary tumor cell lines from rare tumor types are lacking. This protocol describes methods to expand primary tumor cells from surgically resected, malignant pleural mesothelioma (MPM) by providing a complete overview of the process from digestion to enrichment, expansion, cryopreservation, and phenotypic characterization. In addition, this protocol intro ...
cryopreservation, etc ; animal reproduction; boars; semen; sperm quality; viability; Show all 6 Subjects
Abstract:
... This study was conducted to evaluate whether there were differences in viability of cryopreserved semen when using two different freezing (Minitube Cryoguard – F1 or Androstar® CryoPlus – F2) and thawing (Minitube Cryoguard Thawing solution – T1 or Androstar® Plus – T2) extenders. Ejaculates were collected, diluted (1:1), and cooled before shipping at 17 °C overnight. Samples were aliquoted in cry ...
cryopreservation, etc ; ambient temperature; energy; evaporation; liquefaction; prototypes; refrigeration; Show all 7 Subjects
Abstract:
... In this study, a dual mixed-refrigerants refrigeration system coupled with a Linde-Hampson refrigeration (LHR) cycle and a three-stage auto-cascade refrigeration (ACR) cycle was designed and built. The thermodynamic performance of the coupled system was experimentally investigated using low GWP zeotropic mixtures of R1234yf/R32 for the LHR and R170/R14/R50 for the ACR. The experimental results sho ...
Oliver Sampson; Nicholas Lim; Jemima White; Vinicius Vieira; Henrik Kløverpris; Emily Adland; Chris Conlon; Donal Skelly; Lucy Jones; Lizzie Stafford; Anni Jamsen; Ellie Barnes; Susie Dunachie; John Frater; Paul Klenerman; Marcus Altfeld; Philip Goulder
cryopreservation, etc ; blood; cytokines; immune response; innate immunity; vaccination; Show all 6 Subjects
Abstract:
... Central to sex differences observed in outcome from infection and vaccination is the innate immune response, and specifically production of type I interferons by plasmacytoid dendtiric cells (pDCs), the main producers of IFN-α. Evaluation of IFN-α production by pDCs is therefore critical for studies of innate immune function. However, reliable measurement of pDC IFN-α is hampered by reduced cell y ...
cryopreservation, etc ; Cyprinus carpio; absorbance; aquaculture; spectroscopy; sperm concentration; spermatozoa; Show all 7 Subjects
Abstract:
... Absorbance and computer-assisted sperm analysis (CASA) was used to assess the sperm concentration of common carp (Cyprinus carpio) as an alternative to hemocytometer counting. Also the effect of different sperm concentrations on post-thaw sperm parameters and fertilizing capacity were tested. The main goal of the research was to find out whether the tested methods are suitable for fast measurement ...
cryopreservation, etc ; cold storage; cryoprotectants; crystallization; erythrocytes; glycoproteins; humans; ice; Show all 8 Subjects
Abstract:
... Antifreeze proteins (AFPs) and glycoproteins (AFGPs) are exemplary at modifying ice crystal growth and at inhibiting ice recrystallization (IRI) in frozen solutions. These properties make them highly attractive for cold storage and cryopreservation applications of biological tissue, food, and other water-based materials. The specific requirements for optimal cryostorage remain unknown, but high IR ...
cryopreservation, etc ; cerebrospinal fluid; flow cytometry; immunophenotyping; monocytes; pathogenesis; sclerosis; Show all 7 Subjects
Abstract:
... Analysis of cerebrospinal fluid (CSF) represents a valuable window into the pathogenesis of neuroinflammatory diseases, such as multiple sclerosis (MS). However, analysis of the cellular fraction of CSF is often neglected because CSF cells die rapidly ex vivo. Immunophenotyping of CSF cells in multicenter clinical trials requires sample preservation and shipping to a centralized lab. Yet, there is ...
cryopreservation, etc ; biocompatible materials; biotechnology; cold; cold tolerance; cryoprotectants; metabolism; therapeutics; Show all 8 Subjects
Abstract:
... Cryopreservation can help to meet the demand for biosamples of high medical value. However, it remains difficult to effectively cryopreserve some sensitive cells, tissues, and reproductive organs. A coordinated effort from the perspective of the whole frozen biological system is necessary to advance cryopreservation technology. Animals that survive in cold temperatures, such as hibernators and col ...
cryopreservation, etc ; cryoprotectants; glycerol; roosters; semen; sperm motility; sperm quality; straw; Show all 8 Subjects
Abstract:
... The long-term semen cryopreservation is increasingly crucial for conservation of endangered livestock and poultry species. Glycerol is the most widely used cryoprotectant for freezing chicken semen. Continuous improvement in details with glycerol may help increase the fertility of post-thawed semen. Two experiments were performed in the present study to investigate the effects of glycerol concentr ...
cryopreservation, etc ; Perca fluviatilis; aquaculture; commercialization; glucose; methanol; semen; sperm motility; Show all 8 Subjects
Abstract:
... This study emphasis improved semen cryopreservation protocol for commercial scale fertilization in European perch. The optimized procedure involves a new extender (GM 25 K) containing 0.30 M glucose, 7.5% methanol and 25 mM KCl at 3.0 × 10⁹/mL spermatozoa. The supplementation of glucose-methanol extender with 25 mM KCl enabled high sperm motility (54%) up to 30 min post thawing, showing improvemen ...