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- Author:
- Christiane Tröße; Mats Kindt; Steffen Blindheim; Linda Andersen; Are Nylund
- Source:
- Journal of fish diseases 2021 v.44 no.6 pp. 739-745
- ISSN:
- 0140-7775
- Subject:
- cryopreservation, etc ; Paramoeba perurans; amoebic gill disease; aquaculture; fish; parasites; storage time; Show all 7 Subjects
- Abstract:
- ... Paramoeba perurans causes amoebic gill disease (AGD), which is a major problem in aquaculture worldwide. The parasite can be cultured in vitro, but to this date, no method for long‐term storage of the clones exists. In this study, we describe a method for cryopreservation of Paramoeba perurans. The method was successfully employed on four out the five clones we tested. The thawing success rate ...
- DOI:
- 10.1111/jfd.13295
- https://doi.org/10.1111/jfd.13295
- Author:
- Chris O’Brien; Jayeni Hiti-Bandaralage; Raquel Folgado; Alice Hayward; Sean Lahmeyer; Jim Folsom; Neena Mitter
- Source:
- Plants 2021 v.10 no.5 pp. -
- ISSN:
- 2223-7747
- Subject:
- cryopreservation, etc ; avocados; cultivars; ex situ conservation; food security; germplasm; nutrition; protocols; recalcitrant seeds; shoot tips; somatic embryogenesis; tissue culture; Show all 12 Subjects
- Abstract:
- ... Recent development and implementation of crop cryopreservation protocols has increased the capacity to maintain recalcitrant seeded germplasm collections via cryopreserved in vitro material. To preserve the greatest possible plant genetic resources globally for future food security and breeding programs, it is essential to integrate in situ and ex situ conservation methods into a cohesive ...
- DOI:
- 10.3390/plants10050934
- https://doi.org/10.3390/plants10050934
- Author:
- João Pedro Nascimento; Yevhen Horokhovatskyi; Vitaliy Kholodnyy; Marek Rodina; Viktoriya Dzyuba; Taisiya Stechkina; Sergii Boryshpolets; Borys Dzyuba
- Source:
- Aquaculture 2021 v.540 pp. 736682
- ISSN:
- 0044-8486
- Subject:
- cryopreservation, etc ; Acipenser ruthenus; aquaculture; centrifugation; cold storage; sperm concentration; sperm motility; spermatozoa; sturgeon; urine; Show all 10 Subjects
- Abstract:
- ... One of the critical points in low temperature storage is the expense of storage capacities. Moreover, sperm concentration in a sample is not only a parameter for effective use of a cryobanks but also a parameter affecting cryopreservation outcomes. Sturgeon sperm in comparison to other species is significantly less concentrated due to the specificity of sperm maturation process, during which ...
- DOI:
- 10.1016/j.aquaculture.2021.736682
- https://doi.org/10.1016/j.aquaculture.2021.736682
- Author:
- S. Campos; J. Troncoso; E. Paredes
- Source:
- Cryobiology 2021 v.98 pp. 1-4
- ISSN:
- 0011-2240
- Subject:
- cryopreservation, etc ; Paracentrotus lividus; aquaculture; cryobiology; oocytes; spermatozoa; Show all 6 Subjects
- Abstract:
- ... Cryopreservation of gametes, embryos and larvae of marine invertebrates has been investigated in many studies throughout the years. There are many favorable studies on sperm cryopreservation but oocytes are still under research as no successful results have been sustainably obtained for this type of cells. The preservation of both maternal and paternal gametes separately would provide a reliable ...
- DOI:
- 10.1016/j.cryobiol.2020.11.008
- https://doi.org/10.1016/j.cryobiol.2020.11.008
- Author:
- Kazutoshi Nishijima; Shuji Kitajima; Fumikazu Matsuhisa; Manabu Niimi; Chen-chi Wang; Jianglin Fan
- Source:
- Animals 2021 v.11 no.5 pp. -
- ISSN:
- 2076-2615
- Subject:
- cryopreservation, etc ; cold stress; cooling; cryoprotectants; cytoplasm; genome; ice; males; osmotic stress; plasma membrane; rabbits; reactive oxygen species; spermatozoa; Show all 13 Subjects
- Abstract:
- ... The rabbit is a valuable animal for both the economy and biomedical sciences. Sperm cryopreservation is one of the most efficient ways to preserve rabbit strains because it is easy to collect ejaculate repeatedly from a single male and inseminate artificially into multiple females. During the cooling, freezing and thawing process of sperms, the plasma membrane, cytoplasm and genome structures ...
- DOI:
- 10.3390/ani11051220
- https://doi.org/10.3390/ani11051220
- Author:
- Jean Carlos Bettoni; Remi Bonnart; Gayle M. Volk
- Source:
- Plant cell, tissue, and organ culture 2021 v.144 no.1 pp. 21-34
- ISSN:
- 0167-6857
- Subject:
- cryopreservation, etc ; germplasm conservation; human resources; laboratories; literature; materials; organ culture; plant genetic resources; pretreatment; protocols; regrowth; shoot tips; storage; Show all 13 Subjects
- Abstract:
- ... Cryopreservation is a safe approach for the long-term preservation of plant genetic resources that complements traditional field and in vitro germplasm collections. There are many reports of cryopreservation successes in the literature; however, only a fraction of those methods has been routinely used for long-term conservation. A common problem is the difficulty of transferring technology ...
- DOI:
- 10.1007/s11240-020-01846-x
- https://doi.org/10.1007/s11240-020-01846-x
- Author:
- Elena Popova; Mukund Shukla; Haeng-Hoon Kim; Praveen K. Saxena
- Source:
- Plant cell, tissue, and organ culture 2021 v.144 no.1 pp. 49-66
- ISSN:
- 0167-6857
- Subject:
- cryopreservation, etc ; area; bioactive compounds; biosynthesis; cost effectiveness; environment; explants; genetic stability; industry; liquid nitrogen; materials; organ culture; plant genetic resources; protocols; research; root tips; vitrification; Show all 17 Subjects
- Abstract:
- ... Cryopreservation in liquid nitrogen (− 196 °C) ensures safe and cost-effective conservation of plant genetic resources using diverse materials, including roots. To date, successful cryopreservation of root explants isolated from in vitro grown plants as well as adventitious and hairy root cultures has been reported in large numbers of plant species. In particular, significant progress has been ...
- DOI:
- 10.1007/s11240-020-01859-6
- https://doi.org/10.1007/s11240-020-01859-6
- Author:
- Henrik Nausch; Johannes F. Buyel
- Source:
- New biotechnology 2021 v.62 pp. 86-95
- ISSN:
- 1871-6784
- Subject:
- cryopreservation, etc ; cell lines; cell suspension culture; encapsulation; plants (botany); thawing; vitrification; Show all 7 Subjects
- Abstract:
- ... Plant cell cultures can be used as biotechnological platforms for the commercial production of small-molecule active ingredients and recombinant proteins, such as biopharmaceuticals. This requires the cryopreservation of well-characterized cell lines as master cell banks from which uniform working cell banks can be derived to ensure high batch-to-batch reproducibility during production campaigns ...
- DOI:
- 10.1016/j.nbt.2021.02.002
- https://doi.org/10.1016/j.nbt.2021.02.002
- Author:
- Heather J. Gladfelter; Jack Johnston; H. Dayton Wilde; Scott A. Merkle
- Source:
- Plant cell, tissue, and organ culture 2021 v.144 no.1 pp. 211-221
- ISSN:
- 0167-6857
- Subject:
- cryopreservation, etc ; Stewartia; cultivars; endosperm; explants; germplasm; greenhouses; landscaping; mass; organ culture; ornamental value; picloram; plant growth substances; research; somatic embryogenesis; somatic embryos; storage; testing; trade; trees; East Asia; North America; Show all 22 Subjects
- Abstract:
- ... to difficulty with mass propagation. In addition to commercial propagation, there is a need to develop propagation techniques for the conservation of the North American species, which are rare (S. ovata) or endangered (S. malacodendron). This research examined somatic embryogenesis as a means to mass propagate Asian and North American Stewartia species and to provide material for cryopreservation of valuable ...
- DOI:
- 10.1007/s11240-020-01834-1
- https://doi.org/10.1007/s11240-020-01834-1
- Author:
- Yentel Mateo-Otero; Marc Yeste; Anna Damato; Elisa Giaretta
- Source:
- Research in veterinary science 2021 v.135 pp. 20-26
- ISSN:
- 0034-5288
- Subject:
- antioxidants; blastocyst; females; gene expression regulation; genes; germinal vesicle; germplasm; oxidative stress; research; veterinary medicine; vitrification
- Abstract:
- ... Several vitrification protocols have been established for porcine oocytes so as to facilitate gene banking of female germplasm. Although live piglets have been successfully produced from pig oocytes vitrified at the germinal vesicle (GV) stage, the competence of vitrified oocytes to develop into the blastocyst stage is greatly compromised following cryopreservation. The focus of this review ...
- DOI:
- 10.1016/j.rvsc.2020.12.024
- https://doi.org/10.1016/j.rvsc.2020.12.024
- Author:
- Justin D. Tanner; Katheryn Y. Chen; Remi M. Bonnart; Ioannis S. Minas; Gayle M. Volk
- Source:
- Plant cell, tissue, and organ culture 2021 v.144 no.1 pp. 35-48
- ISSN:
- 0167-6857
- Subject:
- cryopreservation, etc ; agronomic traits; crops; decision making; dormancy; gene banks; genetic variation; germplasm conservation; horticulture; labor; plant germplasm; shoot tips; shrubs; trees; winter; Show all 15 Subjects
- Abstract:
- ... Cryopreservation of clonal plant germplasm is a reliable way to preserve important agronomic traits and protect against loss of crop genetic diversity of many horticultural species. Dormant bud cryopreservation techniques present an efficient alternative to the labor-intensive shoot tip cryopreservation process and may allow a single technician to preserve large quantities of germplasm ...
- Handle:
- 10113/7111141
- DOI:
- 10.1007/s11240-020-01884-5
- https://doi.org/10.1007/s11240-020-01884-5
- Author:
- Ayushi Dalmia; Ajay W. Tumaney
- Source:
- Journal of applied phycology 2021 v.33 no.2 pp. 869-878
- ISSN:
- 0921-8971
- Subject:
- cryopreservation, etc ; algology; cell structures; cryoprotectants; dimethyl sulfoxide; ethylene glycol; fatty acid composition; freezers; genetic stability; glycerol; humans; labor; liquid nitrogen; methanol; omega-3 fatty acids; viability; Show all 16 Subjects
- Abstract:
- ... -intensive. Having a cryopreservation technique will be useful for the preservation of A. limacinum cultures. Hence, we explored and developed a cryopreservation technique to preserve A. limacinum. Cryopreservation was done at two different temperatures by using a mechanical freezer (− 80 °C) and liquid nitrogen (− 196 °C). In the mechanical freezer, the cells were preserved in glycerol, while in liquid ...
- DOI:
- 10.1007/s10811-020-02346-3
- https://doi.org/10.1007/s10811-020-02346-3
- Author:
- Christian A. Shirley; Michael E. Colvin; Terrence R. Tiersch; Peter J. Allen
- Source:
- Journal of the World Aquaculture Society 2021 v.52 no.2 pp. 405-417
- ISSN:
- 0893-8849
- Subject:
- cryopreservation, etc ; Pomoxis annularis; Pomoxis nigromaculatus; aquaculture; cryoprotectants; dimethyl sulfoxide; eggs; hatcheries; methanol; phenotype; sperm concentration; sperm motility; spermatozoa; straw; Show all 14 Subjects
- Abstract:
- ... Approaches for white crappie, Pomoxis annularis sperm cryopreservation have led to interest in applying similar methods to black‐stripe black crappie, Pomoxis nigromaculatus. Their rarity in wild populations makes them a preferred phenotype for hatchery use. Sperm cryopreservation procedures were compared between black‐stripe black crappie and white crappie for sperm motility and egg ...
- DOI:
- 10.1111/jwas.12763
- https://doi.org/10.1111/jwas.12763
- Author:
- Christopher S. Andrae; Erika C.S. Oliveira; Marcia A.M.M. Ferraz; Jennifer B. Nagashima
- Source:
- Cryobiology 2021 v.100 pp. 173-179
- ISSN:
- 0011-2240
- Subject:
- cryopreservation, etc ; Canis lupus; Sertoli cells; apoptosis; cell viability; cryobiology; cryoprotectants; dimethyl sulfoxide; ethylene glycol; genomics; liquid nitrogen; males; spermatogonia; sucrose; vitrification; wolves; Show all 16 Subjects
- Abstract:
- ... Development of genomic preservation technologies for canids, especially for seasonally breeding species like the grey wolf (Canis lupus), is needed in advance of growing species conservation concerns. Here, we evaluated the efficacy of two cryopreservation protocols – needle immersion vitrification (NIV) and slow freezing (SF) on grey wolf (n = 7) testicular tissue morphology. NIV samples were ...
- DOI:
- 10.1016/j.cryobiol.2021.01.010
- https://doi.org/10.1016/j.cryobiol.2021.01.010
- Author:
- Monika Trzcińska; Magdalena Bryła
- Source:
- Annals of animal science 2021 v.21 no.2 pp. 513-525
- ISSN:
- 2300-8733
- Subject:
- cryopreservation, etc ; acrosome; animal science; boars; membrane permeability; semen; sperm motility; sperm quality; Show all 8 Subjects
- Abstract:
- ... This study aimed to define potential markers that could determine the suitability of ejaculate for cryopreservation. Fresh semen from eleven boars (4–7 ejaculates/boar), regardless of their sperm motility, was subjected to a cryopreservation procedure. The sperm quality before and after freezing was assessed based on the sperm membrane permeability and acrosome integrity. The results showed ...
- DOI:
- 10.2478/aoas-2020-0095
- https://doi.org/10.2478/aoas-2020-0095
- Author:
- Carla Benelli; Lara S. O. Carvalho; Soumaya EL merzougui; Raffaella Petruccelli
- Source:
- Plants 2021 v.10 no.2 pp. -
- ISSN:
- 2223-7747
- Subject:
- cryopreservation, etc ; Stevia rebaudiana; liquid nitrogen; regrowth; storage time; vitrification; Show all 6 Subjects
- Abstract:
- ... Cryopreservation is a useful tool for the long-term storage of plant genetic resources, and different cryogenic procedures have recently been developed. The present study focused on the use of the Droplet-vitrification (DV) and V cryo-plate protocol for the cryopreservation of Stevia rebaudiana in vitro-derived apical shoot tips and axillary shoot tips. A preliminary test showed that 90 and 120 ...
- DOI:
- 10.3390/plants10020277
- https://doi.org/10.3390/plants10020277
- Author:
- Sang-Eun Jung; Ju-Hee Jin; Jin Seop Ahn; Yong-Hee Kim; Min-Hyung Yun; Seo-Hee Kim; Bang-Jin Kim; Buom-Yong Ryu
- Source:
- Theriogenology 2021 v.159 pp. 165-175
- ISSN:
- 0093-691X
- Subject:
- cryopreservation, etc ; apoptosis; blood serum; fluorescent antibody technique; human fertility; human serum albumin; livestock; mice; stem cells; Show all 9 Subjects
- Abstract:
- ... Cryopreservation of spermatogonial stem cells (SSCs) is a necessity to preserve the genetic information of valuable livestock herds and to produce transgenic animals. However, serum, a key component that allows efficient cryopreservation, has many limitations attributed to its undefined composition, inter-batch variations, and contamination potential. Therefore, we aimed to establish a method ...
- DOI:
- 10.1016/j.theriogenology.2020.10.037
- https://doi.org/10.1016/j.theriogenology.2020.10.037
- Author:
- Małgorzata Maślanka¹; Agnieszka Szewczyk²
- Source:
- Plant cell, tissue, and organ culture 2021 v.144 no.1 pp. 91-95
- ISSN:
- 0167-6857
- Subject:
- cryopreservation, etc ; Tulipa; apical meristems; cold treatment; culture media; explants; organ culture; protocols; regrowth; sucrose; vitrification; Show all 11 Subjects
- Abstract:
- ... The present study provides an efficient cryopreservation protocol for Tulipa tarda cultured in vitro. Apices were excised from bulblets cultivated on MS medium, supplemented with 60 or 90 g l⁻ ¹ sucrose. Half of the bulblets were subjected to a cold treatment at 5 °C for 10 weeks, before exposure of the apices to loading solution (LS) and plant vitrification solution 2 (PVS2). Ten weeks after ...
- DOI:
- 10.1007/s11240-020-01910-6
- https://doi.org/10.1007/s11240-020-01910-6
- Author:
- Nikolas Zuchowicz; Jonathan Daly; Claire Lager; Olivia Williamson; Mary Hagedorn
- Source:
- Cryobiology 2021 v.101 pp. 135-139
- ISSN:
- 0011-2240
- Subject:
- cryopreservation, etc ; corals; cryobiology; spermatozoa; Show all 4 Subjects
- Abstract:
- ... Cryopreservation of coral sperm requires reliable, travel-ready, inexpensive hardware. To this end, we developed and tested a robust, second-generation, conduction-based cryovial cooling rack assembled from 3D-printed and commercially available parts. Cooling rates from −10 to −80 °C were found to be repeatable at −22.9 ± 1.9 (rate ± SD) °C/min for 1-mL samples and −35.4 ± 3.3 °C/min for 0.5-mL ...
- DOI:
- 10.1016/j.cryobiol.2021.04.005
- https://doi.org/10.1016/j.cryobiol.2021.04.005
- Author:
- Tuija Aronen; Susanna Virta; Saila Varis
- Source:
- Plants 2021 v.10 no.2 pp. -
- ISSN:
- 2223-7747
- Subject:
- cryopreservation, etc ; DNA replication; Picea abies; cell senescence; genetic variation; somatic embryogenesis; telomeres; tissue culture; Show all 8 Subjects
- Abstract:
- ... Telomeres i.e., termini of the eukaryotic chromosomes protect chromosomes during DNA replication. Shortening of telomeres, either due to stress or ageing is related to replicative cellular senescence. There is little information on the effect of biotechnological methods, such as tissue culture via somatic embryogenesis (SE) or cryopreservation on plant telomeres, even if these techniques ...
- DOI:
- 10.3390/plants10020416
- https://doi.org/10.3390/plants10020416