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1. Modification of French mini-straw plug position for cryopreservation of small doses of bull sperm

Author:
Lone, Shabir Ahmad; Mohanty, Tushar Kumar; Bhakat, Mukesh; Paray, Adil Rasool; Yadav, Hanuman Prasad; Singh, Ajeet; Sinha, Ranjana; Baithalu, Rubina Kumari; Rahim, Abdul; Kumar, Raj; Kumar, Pradeep; Shah, Nadeem
Source:
Animal reproduction science 2020 v.218 pp. 106485
ISSN:
0378-4320
Subject:
cryopreservation,   etc   ; bulls; cotton; semen; spermatozoa; straw; thawing;     Show all 7 Subjects
Abstract:
... Greater than optimal diluting of semen for producing a large number of doses containing relatively small numbers of sperm can lead to compromised quality of sperm, post-thawing. In the present study the French mini-straw plug position was modified and the effect of re-positioning was evaluated on the quality of sperm after thawing subsequent to cryopreservation of small doses of sperm. Four types ...
DOI:
10.1016/j.anireprosci.2020.106485

2. Growth and albumin secretion of mouse fetal liver cells cryopreserved within porous polymer scaffolds as a viable cell source for bioartificial livers

Author:
Miyoshi, Hirotoshi; Iwamoto, Ayako; Koyama, Toshie
Source:
Journal of bioscience and bioengineering 2020 v.130 no.2 pp. 212-216
ISSN:
1389-1723
Subject:
cryopreservation,   etc   ; albumins; cell viability; hepatocytes; liver; mice; polymers; secretion; thawing;     Show all 9 Subjects
Abstract:
... To clinically apply bioartificial livers (BALs), an effective liver cell cryopreservation method is required for a stable cell supply. In this study, we performed tissue-engineered construct (TEC) cryopreservation of fetal liver cells (FLCs) in which FLCs cultured within a porous polymer scaffold were cryopreserved. Growth and albumin secretion in TEC-cryopreserved FLCs after thawing were compared ...
DOI:
10.1016/j.jbiosc.2020.03.013

3. Microfabrication of low-cost customisable counting chambers for standardised estimation of sperm concentration

Author:
Liu, Yue; Chesnut, Megan; Guitreau, Amy; Beckham, Jacob; Melvin, Adam; Eades, Jason; Tiersch, Terrence R.; Monroe, William Todd
Source:
Reproduction, fertility, and development 2020 v.32 no.9 pp. 873-878
ISSN:
1031-3613
Subject:
cryopreservation,   etc   ; Danio rerio; models; polydimethylsiloxane; prototypes; sperm concentration; sperm quality;     Show all 7 Subjects
Abstract:
... Evaluation of sperm concentration is essential for research and procedures involving AI, cryopreservation and sperm quality assessment. Microfabrication technologies have shown tremendous potential for rapid prototyping and fabrication of devices to assist reproduction and fertility research, but such utility has not yet been made available for most reproduction laboratories. The aim of this study ...
DOI:
10.1071/RD19154

4. Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents

Author:
Jeong, Young-Hoon; Kim, Ukjin; Lee, Seul-Gi; Ryu, Bokyeong; Kim, Jin; Igor, Artyuhov; Kim, Jong Soo; Jung, Cho-Rok; Park, Jae-Hak; Kim, C-Yoon
Source:
BMC biotechnology 2020 v.20 no.1 pp. 45
ISSN:
1472-6750
Subject:
cryopreservation,   etc   ; apoptosis; cell viability; cryoprotectants; cytotoxicity; humans; ice; mesenchymal stromal cells; organoids; stem cells; vitrification;     Show all 11 Subjects
Abstract:
... BACKGROUND: Vitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6–8 M) of cryoprotective agents (CPAs) used in stem cell induce osmotic and metabolic injuries. Moreover, the application of conventional slow-freezing methods to cultures of 3-D organoids of stem cells in various studie ...
DOI:
10.1186/s12896-020-00636-9

5. Demonstration of the cryoprotective properties of the fucose-containing polysaccharide FucoPol

Author:
Guerreiro, Bruno M.; Freitas, Filomena; Lima, João C.; Silva, Jorge C.; Dionísio, Madalena; Reis, Maria A.M.
Source:
Carbohydrate polymers 2020 v.245 pp. 116500
ISSN:
0144-8617
Subject:
cryopreservation,   etc   ; Enterobacter; biodegradability; calorimetry; cryoprotectants; exopolysaccharides; freezing point; ice; melting point; viability;     Show all 10 Subjects
Abstract:
... We report the cryoprotective potential of FucoPol, a fucose-containing bacterial exopolysaccharide produced by Enterobacter A47. In vitro cryopreservation assays of Vero, Saos-2, HFFF2 and C2C12 cell lines exposed to a validated non-cytotoxic 2.5 mg/mL FucoPol concentration demonstrated a consistent post-thaw metabolic viability increase. Calorimetric analysis showed a non-colligative, FucoPol con ...
DOI:
10.1016/j.carbpol.2020.116500

6. Vitrification of caprine secondary and early antral follicles as a perspective to preserve fertility function

Author:
Lopes, Everton Pimentel Ferreira; Rodrigues, Giovanna Quintino; de Brito, Danielle Cristina Calado; Rocha, Rebeca Magalhães Pedrosa; Ferreira, Anna Clara Accioly; de Sá, Naíza Arcângela Ribeiro; Silva, Renato Félix da; de Alcântara, Gabriel Las Heras; Alves, Benner Geraldo; Figueiredo, José Ricardo de; Zelinski, Mary; Rodrigues, Ana Paula Ribeiro
Source:
Reproductive Biology 2020 v.20 no.3 pp. 371-378
ISSN:
1642-431X
Subject:
cryopreservation,   etc   ; aromatase; fluorescence; goats; in vitro culture; ovarian follicles; polymers; staining; viability; vitrification;     Show all 10 Subjects
Abstract:
... The present study aimed to evaluate the structure, survival and development of isolated caprine (secondary-SEC and early antral-EANT) follicles, after vitrification in the presence of synthetic polymers and in vitro culture. Additionally, transzonal projections (TZPs) and p450 aromatase enzyme were evaluated. After isolation, SEC and EANT follicles were in vitro cultured for six days or vitrified. ...
DOI:
10.1016/j.repbio.2020.05.001

7. Long-term Preservation of Micro-algal Stock for Fish Hatcheries

Author:
Chellappan, Ajan; Thangamani, Praba; Markose, Shyni; Thavasimuthu, Citarasu; Thangaswamy, Selvaraj; Mariavincent, Michaelbabu
Source:
Aquaculture reports 2020 v.17 pp. 100329
ISSN:
2352-5134
Subject:
cryopreservation,   etc   ; Nannochloropsis; cryoprotectants; ethylene glycol; fish culture; fish larvae; glycerol; hatcheries; methanol; microalgae; sorbitol; sucrose; viability;     Show all 13 Subjects
Abstract:
... Aquaculture is a fast-growing sector with a high scope for augmentation. Microalgae are frequently used for fish larvae culture. Hatcheries face many problems regarding the maintenance of a stock culture of the microalgae. Long term maintenance of microalgal species by serial sub culturing is often expensive, time-consuming and leads to genetic change. The present study is aimed to develop an alte ...
DOI:
10.1016/j.aqrep.2020.100329

8. Advances in Holding and Cryopreservation of Equine Oocytes and Embryos

Author:
Hinrichs, Katrin
Source:
Journal of equine veterinary science 2020 v.89 pp. 102990
ISSN:
0737-0806
Subject:
cryopreservation,   etc   ; ambient temperature; blastocyst; embryogenesis; intracytoplasmic sperm injection; mares; oocytes; storage temperature; viability;     Show all 9 Subjects
Abstract:
... Methods for holding of oocytes and embryos during shipment as well as for their cryopreservation can greatly aid equine reproductive management. Oocytes can be held at room temperature overnight or at cooler temperatures for two nights without affecting maturation or embryo development after intracytoplasmic sperm injection. In contrast, methods for cryopreservation of equine oocytes that support ...
DOI:
10.1016/j.jevs.2020.102990

9. The proportion of tyrosine phosphorylated spermatozoa in cryopreserved semen is negatively related to crossbred bull fertility

Author:
Vignesh, Kolanjiyappan; Murugavel, Kailasam; Antoine, Dourey; Prakash, Mani Arul; Saraf, Kausthub Kishore; Nag, Pradeep; Karuthadurai, Thirumalaisamy; Kumaresan, Arumugam
Source:
Theriogenology 2020 v.149 pp. 46-54
ISSN:
0093-691X
Subject:
cryopreservation,   etc   ; acrosome; acrosome reaction; bulls; crossbreds; male fertility; phosphorylation; semen; sperm motility; tyrosine;     Show all 10 Subjects
Abstract:
... Sub-fertility is a major problem in crossbred bulls. Identification of subtle differences in the quality of cryopreserved spermatozoa among bulls belonging to different fertility rankings would help determine the latent fertility of semen before their use at field conditions. In the present study, we assessed the status of tyrosine phosphorylation, membrane integrity and acrosome reaction of cryop ...
DOI:
10.1016/j.theriogenology.2020.03.020

10. Morphological features and microtubular changes in vitrified ovine oocytes

Author:
Serra, Elisa; Gadau, Sergio Domenico; Berlinguer, Fiammetta; Naitana, Salvatore; Succu, Sara
Source:
Theriogenology 2020 v.148 pp. 216-224
ISSN:
0093-691X
Subject:
cryopreservation,   etc   ; acetylation; adults; cytoskeleton; females; meiosis; oocytes; parthenogenesis; post-translational modification; puberty; sheep; temperature; tubulin; vitrification;     Show all 14 Subjects
Abstract:
... Cryobanking of oocytes collected from prepubertal donors may supply a virtually unlimited number of female gametes for both basic research and commercial applications. Prepubertal oocytes show some structural and functional limitations compared to the adult ones that may impair their ability to recover damages from cryopreservation. In oocytes, the meiotic spindle is acutely sensitive to temperatu ...
DOI:
10.1016/j.theriogenology.2019.11.007

11. Cryopreservation of feline red blood cells in liquid nitrogen using glycerol and hydroxyethyl starch

Author:
Hon, Marcelle; Thomovsky, Elizabeth J; Brooks, Aimee C; Johnson, Paula A
Source:
Journal of feline medicine and surgery 2020 v.22 no.4 pp. 366-375
ISSN:
1532-2750
Subject:
cryopreservation,   etc   ; cats; cryoprotectants; dogs; erythrocytes; freeze-thaw cycles; glycerol; hematocrit; liquid nitrogen; starch; thawing; viscosity;     Show all 12 Subjects
Abstract:
... The objective of this study was to evaluate the techniques and short-term effects of cryopreservation of feline red blood cells (RBCs) in liquid nitrogen using glycerol or hydroxyethyl starch (HES) as a cryoprotectant. Feline RBCs were manually mixed with either 20% glycerol or 12.5% HES and frozen for 24 h in liquid nitrogen. The samples were thawed and glycerolized samples were manually washed. ...
DOI:
10.1177/1098612X19850932

12. Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depik Rasbora tawarensis (Pisces: Cyprinidae) eggs after short‐term cryopreservation

Author:
Muchlisin, Zainal A.; Sarah, Putri I.; Aldila, Dhea F.; Eriani, Kartini; Hasri, Iwan; Batubara, Agung S.; Nur, Firman M.; Mustaqim, Mustaqim; Muthmainnah, Cut Ruhul; Abinawanto, Abinawanto; Wilkes, Martin
Source:
Aquaculture research 2020 v.51 no.4 pp. 1700-1705
ISSN:
1355-557X
Subject:
cryopreservation,   etc   ; Rasbora; cryoprotectants; dimethyl sulfoxide; egg yolk; eggs; experimental design; fish; hatching; sperm motility; spermatozoa;     Show all 11 Subjects
Abstract:
... Cryoprotectant is the crucial factor in the cryopreservation process. In general, there are two types of cryoprotectant, permeating and non‐permeating cryoprotectants. Dimethyl sulfoxide (DMSO) and egg yolk are common permeating and non‐permeating cryoprotectants respectively. Hence, the objective of the present study was to determine the best proportion of DMSO and egg yolk for the cryopreservati ...
DOI:
10.1111/are.14516

13. Cryopreservation of ram sperm alters the dynamic changes associated with in vitro capacitation

Author:
Peris-Frau, Patricia; Martín-Maestro, Alicia; Iniesta-Cuerda, María; Sánchez-Ajofrín, Irene; Cesari, Andreina; Garde, J. Julián; Villar, Margarita; Soler, Ana J.
Source:
Theriogenology 2020 v.145 pp. 100-108
ISSN:
0093-691X
Subject:
cryopreservation,   etc   ; acrosome; computers; flow cytometry; freeze-thaw cycles; mitochondria; phosphorylation; rams; sperm quality; tyrosine;     Show all 10 Subjects
Abstract:
... The aim of this study was to investigate the dynamic changes that ram sperm experience during in vitro capacitation before and after cryopreservation. Using flow cytometry and computer assisted sperm analysis system (CASA), protein tyrosine phosphorylation and several functional parameters were evaluated in fresh and cryopreserved ram sperm incubated under capacitating and non-capacitating conditi ...
DOI:
10.1016/j.theriogenology.2020.01.046

14. Impact of time between repeated sperm freezing cycles on sperm quality

Author:
Zaghi, Benjamin; Barda, Shimi; Kleiman, Sandra Edith; Hauser, Ron
Source:
Reproductive Biology 2020 v.20 no.1 pp. 75-80
ISSN:
1642-431X
Subject:
cryopreservation,   etc   ; DNA fragmentation; ambient temperature; freeze-thaw cycles; freezing; humans; liquid nitrogen; semen; sperm quality; thawing;     Show all 10 Subjects
Abstract:
... Refreezing of sperm samples would provide the possibility of performing more cycles of fertility treatments. Although the effect of repeated cycles of freezing on sperm quality was studied, the effect of the length of the time interval between each freeze-thaw cycle has not been reported. Hence, we assessed the effect of incubation time on the sperm quality of thawed sperm after repeated freezing. ...
DOI:
10.1016/j.repbio.2019.12.003

15. ‘Semen dilution effect’ on sperm variables and conception rate in buffalo

Author:
Patil, S.; Kumar, Pradeep; Singh, G.; Bala, Renu; Jerome, A.; Patil, C.S.; Kumar, Dharmendra; Singh, Sajjan; Sharma, R.K.
Source:
Animal reproduction science 2020 v.214 pp. 106304
ISSN:
0378-4320
Subject:
cryopreservation,   etc   ; buffaloes; bulls; conception rate; low density lipoprotein; mitochondria; plasma membrane; semen; sperm motility; spermatozoa;     Show all 10 Subjects
Abstract:
... The study aim was to determine how to minimize effects of buffalo semen dilution by evaluating the use of egg yolk (EY), low-density lipoprotein (LDL), and OptiXcell (OC) extenders. Ejaculates (n = 18 from six bulls) were divided into three aliquots that were diluted separately with EY, LDL, and OC extenders corresponding to 20 million (M), 12 M, and 2 M sperm/dose, respectively, and cryopreserved ...
DOI:
10.1016/j.anireprosci.2020.106304

16. Slow Propagation of Ice Binding Limits the Ice-Recrystallization Inhibition Efficiency of PVA and Other Flexible Polymers

Author:
Naullage, Pavithra M.; Molinero, Valeria
Source:
Journal of the American Chemical Society 2020 v.142 no.9 pp. 4356-4366
ISSN:
1520-5126
Subject:
cryopreservation,   etc   ; antifreeze proteins; biomimetics; crystallization; cytotoxicity; frozen foods; ice; mathematical models; molecular weight; polymerization; polyvinyl alcohol;     Show all 11 Subjects
Abstract:
... Ice recrystallization inhibitors (IRI) are of critical importance in biology, cryopreservation of cells and organs, and frozen foods. Antifreeze glycoproteins (AFGPs) are the most potent IRI. Their cost and cytotoxicity drive the design of synthetic flexible polymers that mimic their function. Poly(vinyl alcohol) (PVA) is the most potent biomimetic found to date, although it is orders of magnitude ...
DOI:
10.1021/jacs.9b12943

17. Protein Preferential Solvation in Water:Glycerol Mixtures

Author:
Chéron, Nicolas; Naepels, Margaux; Pluhařová, Eva; Laage, Damien
Source:
TheJournal of physical chemistry 2020 v.124 no.8 pp. 1424-1437
ISSN:
1520-5207
Subject:
cryopreservation,   etc   ; aqueous solutions; glycerol; molecular dynamics; proteins; simulation models; solvation; solvents; temperature;     Show all 9 Subjects
Abstract:
... For proteins in solvent mixtures, the relative abundances of each solvent in their solvation shell have a critical impact on their properties. Preferential solvation of a series of proteins in water–glycerol mixtures is studied here over a broad range of solvent compositions via classical molecular dynamics simulations. Our simulation results reveal that the differences between shell and bulk comp ...
DOI:
10.1021/acs.jpcb.9b11190

18. The role of seminal plasma in the liquid storage of spermatozoa

Author:
Höfner, Lisa; Luther, Anne-Marie; Waberski, Dagmar
Source:
Animal reproduction science 2020 pp. 106290
ISSN:
0378-4320
Subject:
cryopreservation,   etc   ; adverse effects; biomarkers; boars; bulls; male fertility; proteomics; rams; seminal plasma; spermatozoa; stallions;     Show all 11 Subjects
Abstract:
... Ongoing progress in proteomic characterization of seminal plasma has stimulated research on the identification of biomarkers for male fertility and sperm preservability. So far, many studies have evaluated the benefits of reconstituting cryopreserved or sex-sorted semen with seminal plasma. Less information is available about the effect of remaining or added seminal plasma in liquid preserved seme ...
DOI:
10.1016/j.anireprosci.2020.106290

19. Characterization of antisperm antibody binding patterns in relation to sperm phenotypic attributes and field fertility in dairy bulls

Author:
Kuntareddi, C.; Kumaresan, A.; Saraf, K.K.; Nag, P.; Paul, N.; Kurati, S.P.; Selvaraju, S.; Jeyakumar, S.; Manimaran, A.; Ramesha, K.P.; Arangasamy, A.
Source:
Theriogenology 2020 v.141 pp. 161-167
ISSN:
0093-691X
Subject:
cryopreservation,   etc   ; acrosome; antibodies; antigens; calves; conception; crossbreds; dairy bulls; male fertility; phenotype;     Show all 10 Subjects
Abstract:
... To test our hypothesis that antisperm antibodies (ASA) might alter sperm phenotypic attributes thus leading to sub-fertility/infertility in bulls, ASA were generated in crossbred male calves by immunizing with sperm two times. Cryopreserved spermatozoa from crossbred bulls (n = 24) with different field fertility ratings were incubated with ASA and different patterns of ASA immunolocalization were ...
DOI:
10.1016/j.theriogenology.2019.09.022

20. The effect of cryopreservation extender on sperm motility and hatch success in northern pike (Esox lucius)

Author:
Cejko, Beata Irena; Sarosiek, Beata; Dryl, Katarzyna; Judycka, Sylwia; Szczepkowska, Bożena; Szczepkowski, Mirosław; Kowalski, Radosław Kajetan
Source:
Aquaculture 2020 v.514 pp. 734482
ISSN:
0044-8486
Subject:
cryopreservation,   etc   ; Esox lucius; eggs; hatching; ice; longevity; methanol; pH; potassium chloride; sperm motility; spermatozoa; thawing; trehalose;     Show all 13 Subjects
Abstract:
... Northern pike (Esox lucius) sperm equilibration and cryopreservation were conducted using a simple cryopreservation extender (CE) containing 185 mM trehalose, 40 mM KCl, 20 mM Tris at pH 8.5 and supplemented with 10% of methanol. Sperm was diluted tenfold (1:9; sperm:CE), and a subset of the samples was equilibrated (over 15 min) and stored on crushed ice (+4 °C). The remainder of the samples were ...
DOI:
10.1016/j.aquaculture.2019.734482