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You searched for: Subject "cryopreservation" Remove constraint Subject: "cryopreservation"
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1. High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp

Author:
Anatolii Sotnikov; Marek Rodina; Taisiya Stechkina; Cristiane Fernanda Benevente; David Gela; Sergii Boryshpolets; Vitaliy Kholodnyy; Otomar Linhart; Borys Dzyuba
Source:
Aquaculture 2023 v.562 pp. 738746
ISSN:
0044-8486
Subject:
cryopreservation,   etc   ; Cyprinus carpio; carp; cryoprotectants; eggs; embryogenesis; sperm concentration; sperm motility; spermatozoa;     Show all 9 Subjects
Abstract:
... To avoid mechanical compression of spermatozoa during the cryopreservation, a high dilution ratio with the cryoprotective medium is applied. Due to this, the sample volume increases, which entails increased cryobank capacity. Moreover, the high volume of cryomedias does not allow to fertilize of large volumes of eggs in practical artificial reproduction of common carp aquaculture. The current stud ...
DOI:
10.1016/j.aquaculture.2022.738746

2. Ice recrystallization inhibition activity in bile salts

Author:
Zhihong Wang; Min Li; Tao Wu
Source:
Journal of colloid and interface science 2023 v.629 pp. 728-738
ISSN:
0021-9797
Subject:
cryopreservation,   etc   ; bile; cryoprotectants; crystallization; deoxycholic acid; gelation; hydrophilicity; hysteresis; ice; ice nucleation; liquid crystals; micelles; nanomaterials; sodium; sodium cholate; temperature; viscosity;     Show all 17 Subjects
Abstract:
... Ice recrystallization inhibitors are novel cryoprotective agents that can reduce the freezing damage of cells, tissues, and organs in cryopreservation. To date, potent ice recrystallization inhibition (IRI) activity has been found on antifreeze (glyco)proteins, polymers, nanomaterials, and a limited number of chemically synthesized small molecules. This paper reports a relatively potent IRI activi ...
DOI:
10.1016/j.jcis.2022.09.102

3. Basic and applied features in the cryopreservation progress of bovine embryos

Author:
Roniele Santana Valente; Thamiris Vieira Marsico; Mateus José Sudano
Source:
Animal reproduction science 2022 v.239 pp. 106970
ISSN:
0378-4320
Subject:
cryopreservation,   etc   ; cattle; markets; pregnancy rate; vitrification;     Show all 5 Subjects
Abstract:
... The expansion of the use of in vitro production techniques has revolutionized the bovine embryo market. In the last decade, we have seen the number of in vitro produced (IVP) embryos surpass the number of in vivo-derived (IVD) embryos obtained worldwide. Concomitantly, other biotechnologies were also improved, following the global trend. Embryo cryopreservation has received special attention, as i ...
DOI:
10.1016/j.anireprosci.2022.106970

4. Macrocycle molecule-based cryoprotectants for ice recrystallization inhibition and cell cryopreservation

Author:
Bing Hu; Guo-Kai Li; Guo-Min Ai; Man Zhang; Shan-Shan Su; Xu He; Xiao-Liu Li; Ke-Rang Wang
Source:
Journal of materials chemistry B 2022 v.10 no.36 pp. 6922-6927
ISSN:
2050-7518
Subject:
cryopreservation,   etc   ; cryoprotectants; crystallization; ice; light microscopes;     Show all 5 Subjects
Abstract:
... Cyclodextrin-based cryoprotectants were developed. α-TMCD, which can be easily put into large-scale production, showed enhanced cell viabilities of 19.97 ± 0.78%, 13.93 ± 4.46% and 19.10 ± 0.95% against GES-1, hucMSCs and A549 cells. Moreover, the viable cells observed by light microscope imaging showed that the enhanced hucMSC cell number percentage of α-TMCD was 103.2%. An α-TMCD-DMSO-based CPA ...
DOI:
10.1039/d2tb01083f

5. Bioinspired Ice-Binding Materials for Tissue and Organ Cryopreservation

Author:
Zhang Liu; Xia Zheng; Jianjun Wang
Source:
Journal of the American Chemical Society 2022 v.144 no.13 pp. 5685-5701
ISSN:
1520-5126
Subject:
cryopreservation,   etc   ; cryoprotectants; cytotoxicity; ice; ice nucleation; medicine;     Show all 6 Subjects
Abstract:
... Cryopreservation of tissues and organs can bring transformative changes to medicine and medical science. In the past decades, limited progress has been achieved, although cryopreservation of tissues and organs has long been intensively pursued. One key reason is that the cryoprotective agents (CPAs) currently used for cell cryopreservation cannot effectively preserve tissues and organs because of ...
DOI:
10.1021/jacs.2c00203

6. Different occurrence rates of centrally located cytoplasmic granulation in one cohort oocytes show distinctive embryo competence and clinical outcomes

Author:
Fangyuan Sun; Jintao Cun; Rongfang Huang; Yunyan Chen; Gideon Verwoerd; Yuansong Yu
Source:
Reproductive Biology 2022 v.22 no.3 pp. 100649
ISSN:
1642-431X
Subject:
cryopreservation,   etc   ; blastocyst; eggs; embryo transfer; humans; oocytes;     Show all 6 Subjects
Abstract:
... Centrally located cytoplasmic granulation (central granulation) is a common cytoplasmic dysmorphism in human oocytes retrieved after controlled ovarian hyperstimulation (COH). In order to achieve a better understanding of its formation and effects on clinical outcomes, we retrospectively analyzed 422 ICSI treatment cycles. Three groups of patients were classified according to the ratio of central ...
DOI:
10.1016/j.repbio.2022.100649

7. New Challenges in Cryopreservation: A Reproductive Perspective

Author:
Daniela Bebbere; Sara Succu
Source:
Animals 2022 v.12 no.13 pp. -
ISSN:
2076-2615
Subject:
cryopreservation,   etc   ; animals; cell structures; cold storage; tissues;     Show all 5 Subjects
Abstract:
... Cryopreservation is a fundamental procedure to preserve the structure and function of cells and tissues by storing them at low temperatures for long periods [...] ...
DOI:
10.3390/ani12131598

8. The Tip of the Iceberg: Cryopreservation Needs for Meeting the Challenge of Exceptional Plant Conservation

Author:
Valerie C. Pence; Emily Beckman Bruns
Source:
Plants 2022 v.11 no.12 pp. -
ISSN:
2223-7747
Subject:
cryopreservation,   etc   ; Dipterocarpaceae; Pittosporaceae; Rhizophoraceae; herbaceous plants; pollen;     Show all 6 Subjects
Abstract:
... Cryopreservation is increasingly important as a conservation tool, particularly for threatened exceptional species. The goal of this study was to investigate the current knowledge of plant cryopreservation through a search of the literature in Web of Science and align that with the 775 species currently identified on the Working List of Exceptional Plants. While there is a good foundation in plant ...
DOI:
10.3390/plants11121528

9. Calculation of a temperature–volume phase diagram of water to inform the study of isochoric freezing down to cryogenic temperatures

Author:
Matthew J. Powell-Palm
Source:
RSC advances 2022 v.12 no.32 pp. 20603-20609
ISSN:
2046-2069
Subject:
cryopreservation,   etc   ; Gibbs free energy; evolution; ice; metrology; temperature;     Show all 6 Subjects
Abstract:
... Phase diagrams are integral to the application and interpretation of materials thermodynamics, and none is more ubiquitous than the common temperature–pressure diagram of water and its many icy phases. Inspired by recent advances in isochoric thermodynamics, we here employ a simple convex hull approach to efficiently calculate an updated temperature–volume phase diagram for water and five of its s ...
DOI:
10.1039/d2ra03683e

10. Effect of Linoleic Acid on the Efficiency of Mouse Embryos’ Vitrification

Author:
E. Yu. Brusentsev; T. N. Igonina; S. V. Okotrub; E. A. Chuyko; S. Ya. Amstislavsky
Source:
Russian journal of developmental biology 2022 v.53 no.1 pp. 51-54
ISSN:
1062-3604
Subject:
cryopreservation,   etc   ; fluorescence microscopy; linoleic acid; mice; viability; vitrification;     Show all 6 Subjects
Abstract:
... The study examines the effect of in vitro cultivation of mouse embryos for 48 h with 200 µM linoleic acid (LA) on their viability after vitrification. Fluorescence microscopy of DAPI-stained embryos showed increased fragmentation of nuclei in case of exposure to LA without cryopreservation and a significant decrease in the number of cells in vitrified embryos 24 h after their thawing. Thus, in vit ...
DOI:
10.1134/S1062360422010027

11. Generation and expansion of primary, malignant pleural mesothelioma tumor lines

Author:
Griffiths Tamara M.; Ramos Carlos; Haymaker Cara L.
Source:
Journal of visualized experiments 2022 no.182 pp. e63374
ISSN:
1940-087X
Subject:
cryopreservation,   etc   ; adhesion; digestion; dissociation; mesothelioma; neoplasm cells; phenotype;     Show all 7 Subjects
Abstract:
... Current methodologies for the expansion of primary tumor cell lines from rare tumor types are lacking. This protocol describes methods to expand primary tumor cells from surgically resected, malignant pleural mesothelioma (MPM) by providing a complete overview of the process from digestion to enrichment, expansion, cryopreservation, and phenotypic characterization. In addition, this protocol intro ...
DOI:
10.3791/63374

12. In vitro effects of two different commercial freezing and thawing extenders on boar sperm quality

Author:
AFC De Andrade; R Grossfeld; RV Knox
Source:
Animal reproduction science 2022 v.236 pp. 106906
ISSN:
0378-4320
Subject:
cryopreservation,   etc   ; animal reproduction; boars; semen; sperm quality; viability;     Show all 6 Subjects
Abstract:
... This study was conducted to evaluate whether there were differences in viability of cryopreserved semen when using two different freezing (Minitube Cryoguard – F1 or Androstar® CryoPlus – F2) and thawing (Minitube Cryoguard Thawing solution – T1 or Androstar® Plus – T2) extenders. Ejaculates were collected, diluted (1:1), and cooled before shipping at 17 °C overnight. Samples were aliquoted in cry ...
DOI:
10.1016/j.anireprosci.2021.106906

13. Experimental characterization of an innovative refrigeration system coupled with Linde-Hampson cycle and auto-cascade cycle for multi-stage refrigeration temperature applications

Author:
Yanbin Qin; Nanxi Li; Hua Zhang; Binhui Jin; Baolin Liu
Source:
Energy 2022 v.240 pp. 122498
ISSN:
0360-5442
Subject:
cryopreservation,   etc   ; ambient temperature; energy; evaporation; liquefaction; prototypes; refrigeration;     Show all 7 Subjects
Abstract:
... In this study, a dual mixed-refrigerants refrigeration system coupled with a Linde-Hampson refrigeration (LHR) cycle and a three-stage auto-cascade refrigeration (ACR) cycle was designed and built. The thermodynamic performance of the coupled system was experimentally investigated using low GWP zeotropic mixtures of R1234yf/R32 for the LHR and R170/R14/R50 for the ACR. The experimental results sho ...
DOI:
10.1016/j.energy.2021.122498

14. A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells

Author:
Oliver Sampson; Nicholas Lim; Jemima White; Vinicius Vieira; Henrik Kløverpris; Emily Adland; Chris Conlon; Donal Skelly; Lucy Jones; Lizzie Stafford; Anni Jamsen; Ellie Barnes; Susie Dunachie; John Frater; Paul Klenerman; Marcus Altfeld; Philip Goulder
Source:
Journal of immunological methods 2022 v.504 pp. 113263
ISSN:
0022-1759
Subject:
cryopreservation,   etc   ; blood; cytokines; immune response; innate immunity; vaccination;     Show all 6 Subjects
Abstract:
... Central to sex differences observed in outcome from infection and vaccination is the innate immune response, and specifically production of type I interferons by plasmacytoid dendtiric cells (pDCs), the main producers of IFN-α. Evaluation of IFN-α production by pDCs is therefore critical for studies of innate immune function. However, reliable measurement of pDC IFN-α is hampered by reduced cell y ...
DOI:
10.1016/j.jim.2022.113263

15. Adjustment of common carp sperm concentration prior to cryopreservation: Does it matter?

Author:
Bernadett Pataki; Ákos Horváth; Gergely Mészáros; Nevena Kitanović; András Ács; Árpád Hegyi; József Molnár; Balázs Csorbai; Béla Urbányi
Source:
Aquaculture reports 2022 v.24 pp. 101109
ISSN:
2352-5134
Subject:
cryopreservation,   etc   ; Cyprinus carpio; absorbance; aquaculture; spectroscopy; sperm concentration; spermatozoa;     Show all 7 Subjects
Abstract:
... Absorbance and computer-assisted sperm analysis (CASA) was used to assess the sperm concentration of common carp (Cyprinus carpio) as an alternative to hemocytometer counting. Also the effect of different sperm concentrations on post-thaw sperm parameters and fertilizing capacity were tested. The main goal of the research was to find out whether the tested methods are suitable for fast measurement ...
DOI:
10.1016/j.aqrep.2022.101109

16. Ice Recrystallization Inhibition Is Insufficient to Explain Cryopreservation Abilities of Antifreeze Proteins

Author:
Yuling Sun; Daria Maltseva; Jie Liu; Theordore Hooker; Volker Mailänder; Hans Ramløv; Arthur L. DeVries; Mischa Bonn; Konrad Meister
Source:
Biomacromolecules 2022 v.23 no.3 pp. 1214-1220
ISSN:
1526-4602
Subject:
cryopreservation,   etc   ; cold storage; cryoprotectants; crystallization; erythrocytes; glycoproteins; humans; ice;     Show all 8 Subjects
Abstract:
... Antifreeze proteins (AFPs) and glycoproteins (AFGPs) are exemplary at modifying ice crystal growth and at inhibiting ice recrystallization (IRI) in frozen solutions. These properties make them highly attractive for cold storage and cryopreservation applications of biological tissue, food, and other water-based materials. The specific requirements for optimal cryostorage remain unknown, but high IR ...
DOI:
10.1021/acs.biomac.1c01477

17. Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials

Author:
Ina Mexhitaj; Noha Lim; Jose I. Fernandez-Velasco; Tobias Zrzavy; Kristina M. Harris; Paolo A. Muraro; Luisa M. Villar; Amit Bar-Or; Laura A. Cooney
Source:
Journal of immunological methods 2022 v.510 pp. 113344
ISSN:
0022-1759
Subject:
cryopreservation,   etc   ; cerebrospinal fluid; flow cytometry; immunophenotyping; monocytes; pathogenesis; sclerosis;     Show all 7 Subjects
Abstract:
... Analysis of cerebrospinal fluid (CSF) represents a valuable window into the pathogenesis of neuroinflammatory diseases, such as multiple sclerosis (MS). However, analysis of the cellular fraction of CSF is often neglected because CSF cells die rapidly ex vivo. Immunophenotyping of CSF cells in multicenter clinical trials requires sample preservation and shipping to a centralized lab. Yet, there is ...
DOI:
10.1016/j.jim.2022.113344

18. Bioinspired materials and technology for advanced cryopreservation

Author:
Mengjia Dou; Chennan Lu; Wei Rao
Source:
Trends in biotechnology 2022 v.40 no.1 pp. 93-106
ISSN:
0167-7799
Subject:
cryopreservation,   etc   ; biocompatible materials; biotechnology; cold; cold tolerance; cryoprotectants; metabolism; therapeutics;     Show all 8 Subjects
Abstract:
... Cryopreservation can help to meet the demand for biosamples of high medical value. However, it remains difficult to effectively cryopreserve some sensitive cells, tissues, and reproductive organs. A coordinated effort from the perspective of the whole frozen biological system is necessary to advance cryopreservation technology. Animals that survive in cold temperatures, such as hibernators and col ...
DOI:
10.1016/j.tibtech.2021.06.004

19. Effect of glycerol concentration, glycerol removal method, and straw type on the quality and fertility of frozen chicken semen

Author:
Yunhe Zong; Yanyan Sun; Yunlei Li; Gamal M.K. Mehaisen; Jingwei Yuan; Hui Ma; Aixin Ni; Yuanmei Wang; Shaimaa K. Hamad; Ahmed M. Elomda; Ahmed O. Abbas; Jilan Chen
Source:
Poultry science 2022 v.101 no.6 pp. 101840
ISSN:
0032-5791
Subject:
cryopreservation,   etc   ; cryoprotectants; glycerol; roosters; semen; sperm motility; sperm quality; straw;     Show all 8 Subjects
Abstract:
... The long-term semen cryopreservation is increasingly crucial for conservation of endangered livestock and poultry species. Glycerol is the most widely used cryoprotectant for freezing chicken semen. Continuous improvement in details with glycerol may help increase the fertility of post-thawed semen. Two experiments were performed in the present study to investigate the effects of glycerol concentr ...
DOI:
10.1016/j.psj.2022.101840

20. Toward commercialization: Improvement of a semen cryopreservation protocol for European perch enables its implementation for commercial-scale fertilization

Author:
S. Judycka; D. Żarski; M.A. Dietrich; H. Karol; P. Hliwa; M. Błażejewski; A. Ciereszko
Source:
Aquaculture 2022 v.549 pp. 737790
ISSN:
0044-8486
Subject:
cryopreservation,   etc   ; Perca fluviatilis; aquaculture; commercialization; glucose; methanol; semen; sperm motility;     Show all 8 Subjects
Abstract:
... This study emphasis improved semen cryopreservation protocol for commercial scale fertilization in European perch. The optimized procedure involves a new extender (GM 25 K) containing 0.30 M glucose, 7.5% methanol and 25 mM KCl at 3.0 × 10⁹/mL spermatozoa. The supplementation of glucose-methanol extender with 25 mM KCl enabled high sperm motility (54%) up to 30 min post thawing, showing improvemen ...
DOI:
10.1016/j.aquaculture.2021.737790