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You searched for: Subject "cytoplasm" Remove constraint Subject: "cytoplasm"
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1. Biomineralized Zeolitic Imidazolate Framework-8 Nanoparticles Enable Polymerase-Driven DNA Biocomputing for Reliable Cell Identification

Author:
Dan-Dan Wang; Juan Zhang; Qiao-Qin Yu; Ke Zhang; Ting-Ting Chen; Xia Chu
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4794-4802
ISSN:
1520-6882
Subject:
cytoplasm,   etc   ; DNA; analytical chemistry; microRNA; therapeutics;     Show all 5 Subjects
Abstract:
... Investigating multiple miRNA expression patterns in living cells by DNA logic biocomputing is a valuable strategy for diagnosis and biomedical studies. The introduction of protein enzymes in DNA logic biocomputing circuits not only expands the toolbox of nucleic acid assembly techniques, but also further improves the specificity of recognizing and processing of DNA input. Herein, a polymerase-driv ...
DOI:
10.1021/acs.analchem.1c05605

2. Non-monotonic fibril surface occlusion by GFP tags from coarse-grained molecular simulations

Author:
Julian C. Shillcock; Janna Hastings; Nathan Riguet; Hilal A. Lashuel
Source:
Computational and Structural Biotechnology Journal 2022 v.20 pp. 309-321
ISSN:
2001-0370
Subject:
cytoplasm,   etc   ; amyloid; biotechnology; brain; fluorescence; pathogenicity; polymerization;     Show all 7 Subjects
Abstract:
... The pathological growth of amyloid fibrils in neurons underlies the progression of neurodegenerative diseases including Alzheimer’s and Parkinson’s disease. Fibrils form when soluble monomers oligomerise in the cytoplasm. Their subsequent growth occurs via nucleated polymerization mechanisms involving the free ends of the fibrils augmented by secondary nucleation of new oligomers at their surface. ...
DOI:
10.1016/j.csbj.2021.12.017

3. A Difference between In Vitro and In-Cell Protein Dimer Formation

Author:
I-Te Chu; Claire J. Stewart; Shannon L. Speer; Gary J. Pielak
Source:
Biochemistry 2022 v.61 no.6 pp. 409-412
ISSN:
1520-4995
Subject:
cytoplasm,   etc   ; Escherichia coli; nuclear magnetic resonance spectroscopy; urea;     Show all 4 Subjects
Abstract:
... The high concentration of macromolecules in cells affects the stability of proteins and protein complexes via hard repulsions and chemical interactions, yet few studies have focused on chemical interactions. We characterized the domain-swapped dimer of the B1 domain of protein G in buffer and Escherichia coli cells by using heteronuclear, multidimensional nuclear magnetic resonance spectroscopy. I ...
DOI:
10.1021/acs.biochem.1c00780

4. Three‐dimensional architecture of Cyrilia lignieresi gametocyte‐stage development inside red blood cells

Author:
Maíra Turiel‐Silva; Camila Wendt; Edilene O. Silva; Ana Paula Drummond Rodrigues; Wanderley de Souza; Kildare Miranda; José A. P. Diniz
Source:
journal of eukaryotic microbiology 2022 v.69 no.3 pp. e12894
ISSN:
1066-5234
Subject:
cytoplasm,   etc   ; Haemogregarinidae; Protozoa; erythrocytes; fish; microbiology; parasites; tomography;     Show all 8 Subjects
Abstract:
... The Haemogregarinidae family (Apicomplexa: Adeleina) comprises hemoprotozoa that infect mammals, birds, amphibians, fish, and reptiles. Some morphological characteristics of the Cyrilia lignieresi have been described previously, but the parasite–erythrocyte relationship is still poorly understood. In order to understand the structural architecture of C. lignieresi‐infected red blood cells, electro ...
DOI:
10.1111/jeu.12894

5. Arabidopsis exocyst subunit SEC6 is involved in cell plate formation during Microgametogenesis

Author:
Xiaoyun Tan; Haoyue Xu; Jie Ye; Jin Wang; Wenlong Liu; Feng Liu; Qingchen Rui; Yiqun Bao
Source:
Biochemical and biophysical research communications 2022 v.598 pp. 100-106
ISSN:
0006-291X
Subject:
cytoplasm,   etc   ; Arabidopsis; cytokinesis; gametophytes; males; mitosis; mutants; mutation; pollen; research;     Show all 10 Subjects
Abstract:
... Cytokinesis during pollen mitosis I is critical for cell division and differentiation in the male gametophyte development, but the vesicle trafficking mechanisms in this process are largely unknown. Exocyst is an octameric tethering complex which plays multiple important roles in plant cell vesicle trafficking. Here we report the characterization of exocyst subunit SEC6 in the cytokinesis during p ...
DOI:
10.1016/j.bbrc.2022.01.092

6. Seasonal expression of extracellular signal regulated kinases in the colon of wild ground squirrels (Spermophilus dauricus)

Author:
Yue Song; Xiaoying Yang; Xueying Zhang; Jueyu Zhu; Yixin Chen; Fuli Gao; Haolin Zhang; Yingying Han; Qiang Weng; Zhengrong Yuan
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2209-2215
ISSN:
0301-4851
Subject:
cytoplasm,   etc   ; Spermophilus; colon; immunohistochemistry; molecular biology; quantitative polymerase chain reaction;     Show all 6 Subjects
Abstract:
... BACKGROUND: The purpose of the experiment was to explore the localization and seasonal expression of extracellular signal regulated kinase (ERK) in the colonic tissue of wild ground squirrels (Spermophilus dauricus). METHODS AND RESULTS: Hematoxylin–eosin staining, immunohistochemistry, real-time quantitative PCR and Western blotting were used in this experiment. The histological results showed th ...
DOI:
10.1007/s11033-021-07042-0

7. Biochemical changes in the cytoplasm of bovine oocytes during the in vitro maturation process: a Raman microscopy study

Author:
Luis Emanuel Jimenez; Ana Carolina Juárez; Mariela Roldán-Olarte; Rosa María Susana Álvarez
Source:
Veterinary research communications 2022 v.46 no.2 pp. 549-562
ISSN:
0165-7380
Subject:
cytoplasm,   etc   ; cattle; lipids; microscopy; oocytes; protein content; veterinary medicine; zona pellucida;     Show all 8 Subjects
Abstract:
... The sequence and chronology of the main biochemical changes occurring in the cytoplasm of bovine oocytes during the in vitro maturation process were tracked by Raman microscopy applied to cells previously subjected to enzymatic digestion of the zona pellucida. Specific spectral markers for proteins, lipids and carbohydrates were used to evaluate the developmental status of the ooplasm at four diff ...
DOI:
10.1007/s11259-021-09882-4

8. Long Intergenic Non-Protein Coding RNA 02381 Promotes the Proliferation and Invasion of Ovarian Endometrial Stromal Cells through the miR-27b-3p/CTNNB1 Axis

Author:
Xiaoqing Wang; Peili Wu; Cheng Zeng; Jingwen Zhu; Yingfang Zhou; Ye Lu; Qing Xue
Source:
Genes 2022 v.13 no.3 pp. -
ISSN:
2073-4425
Subject:
cytoplasm,   etc   ; bioinformatics; cell proliferation; endometriosis; endometrium; hybridization; luciferase; plasmids; transcription (genetics);     Show all 9 Subjects
Abstract:
... Purpose: Catenin Beta 1 (CTNNB1) is a key regulator of cell proliferation and invasion in endometriosis; however, its upstream factor is not clear. Long noncoding RNAs may participate in endometriosis. The aim of this study was to investigate the mechanism of interaction between LINC02381 and CTNNB1 in endometriosis. Method: Screening and validation of RNAs were completed by whole transcriptional ...
DOI:
10.3390/genes13030433

9. Morphology and Molecular Phylogeny of Fuscheriides baugilensis sp. nov. (Protozoa, Ciliophora, Haptorida) from South Korea

Author:
Seok Won Jang; Atef Omar; Seung Won Nam; Jae-Ho Jung
Source:
Diversity 2022 v.14 no.2 pp. -
ISSN:
1424-2818
Subject:
cytoplasm,   etc   ; Haptorida; Protozoa; body size; genes; new species; paraphyly; South Korea;     Show all 8 Subjects
Abstract:
... The morphology and molecular phylogeny of a new haptorid ciliate, Fuscheriides baugilensis sp. nov., discovered in a temporary pond in South Korea, were investigated. The new species is characterized by its small body size (30–55 × 15–20 μm in vivo), oblong to rod-shaped extrusomes in the oral bulge and cytoplasm, 14–16 somatic kineties, two dorsal brush rows, and single subapical ciliary condensa ...
DOI:
10.3390/d14020070

10. Sequencing-free Analysis of Multiple Methylations on Gene-Specific mRNAs

Author:
Xi Zhao; Xianglin Ji; Jin Qu; Kai Xie; Zixun Wang; Peilin Fang; Yuan Wang; Youyang Wan; Yang Yang; Wenjun Zhang; Peng Shi
Source:
Journal of the American Chemical Society 2022 v.144 no.13 pp. 6010-6018
ISSN:
1520-5126
Subject:
cytoplasm,   etc   ; epigenetics; gene expression; genes; mammals; methylation; quantitative analysis; stress response;     Show all 8 Subjects
Abstract:
... RNA epigenetics is a new layer of mechanism to regulate gene expression, but limited techniques are available to profile the status of mRNA modifications. Here, we describe a molecule proximity-based technique for simultaneous analysis of multiple types of mRNA methylation with specific gene information in living cells. N⁶-methyladenosine (m⁶A) or N¹-methyladenosine (m¹A) modifications on multiple ...
DOI:
10.1021/jacs.2c01036

11. The roles of diol dehydratase from pdu operon on glycerol catabolism in Klebsiella pneumoniae

Author:
Lin Shu; Qinghui Wang; Weiyan Jiang; Marina Tišma; Beakrock Oh; Jiping Shi; Gary J. Lye; Frank Baganz; Dong Wei; Jian Hao
Source:
Enzyme and microbial technology 2022 v.157 pp. 110021
ISSN:
0141-0229
Subject:
cytoplasm,   etc   ; Klebsiella pneumoniae; catabolism; culture media; glycerol; operon; propanediol dehydratase; technology;     Show all 8 Subjects
Abstract:
... The dha operon of Klebsiella pneumoniae is responsible for glycerol catabolism and 1,3-propanediol formation. Subunits of glycerol dehydratase and the large subunit of glycerol dehydratase reactivating factor are encoded by dhaBCE and dhaF, respectively. Proteins of pdu operon form a microcompartment (bacteria organelle) and responsible for 1,2-propanediol catabolism. In this operon, pduCDE and pd ...
DOI:
10.1016/j.enzmictec.2022.110021

12. Kinetic Studies of the Activation of Bordetella pertussis Adenylate Cyclase by Calmodulin

Author:
Ross L. Stein
Source:
Biochemistry 2022 v.61 no.7 pp. 554-562
ISSN:
1520-4995
Subject:
cytoplasm,   etc   ; Bordetella pertussis; adenosine monophosphate; adenylate cyclase; calmodulin; cough; lungs; toxins; virulence;     Show all 9 Subjects
Abstract:
... Adenylate cyclase toxin (ACT) is a virulence factor secreted by Bordetella pertussis and plays a causative role in whooping cough. After ACT attaches to lung phagocytes, the adenylate cyclase (AC) domain of the toxin is transported into the cytoplasm where it is activated by calmodulin (CaM) to cyclize ATP into 3′,5′-cyclic adenosine monophosphate (cAMP). Production of high concentrations of cAMP ...
DOI:
10.1021/acs.biochem.1c00824

13. Presence of two RIG-I-like receptors, MDA5 and LGP2, and their dsRNA binding capacity in a perciform fish, the snakehead Channa argus

Author:
Lan Hao Liu; Yong-An Zhang; P. Nie; Shan Nan Chen
Source:
Developmental and comparative immunology 2022 v.126 pp. 104235
ISSN:
0145-305X
Subject:
cytoplasm,   etc   ; Channa argus; Clupeiformes; Polypteriformes; databases; fish; genes; genomics; innate immunity; phylogeny;     Show all 10 Subjects
Abstract:
... Fish retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) are critical RNA sensors in cytoplasm and are involved in antiviral innate immunity. However, some species of fish lack RIG-I gene, and the function of RLR members in RIG-I-absent fish is poorly understood. In the present study, MDA5, LGP2 and MAVS genes were identified in commercially important snakehead Channa argus. But, RIG-I ge ...
DOI:
10.1016/j.dci.2021.104235

14. Ultrastructure of the larval eyes of the hangingfly Terrobittacus implicatus (Mecoptera: Bittacidae)

Author:
Ying-Ying Zheng; Qing-Xiao Chen; Qi Yi; Bao-Zhen Hua
Source:
Micron 2022 v.152 pp. 103176
ISSN:
0968-4328
Subject:
cytoplasm,   etc   ; Bittacidae; Panorpidae; cornea; evolution; larvae; ommatidia; photoreceptors; transmission electron microscopy; ultrastructure;     Show all 10 Subjects
Abstract:
... The fine structure of the larval eyes of the hangingfly Terrobittacus implicatus (Huang & Hua) was investigated using scanning and transmission electron microscopy. The results show that the larval eyes of T. implicatus each consist of seven spaced ommatidia. Each ommatidium is composed of a corneal lens with about 45 lamellae, a tetrapartite eucone type of crystalline cone, eight retinula cells, ...
DOI:
10.1016/j.micron.2021.103176

15. A novel putative 2-oxoglutarate-dependent dioxygenase gene (BoaAOP-like) regulates aliphatic glucosinolate biosynthesis in Chinese kale

Author:
Hao Zheng; Yating Wang; Xiangxiang Li; Wenli Huang; Huiying Miao; Huanxiu Li; Qiaomei Wang; Bo Sun; Fen Zhang
Source:
Scientia horticulturae 2022 v.297 pp. 110921
ISSN:
0304-4238
Subject:
cytoplasm,   etc   ; Brassica oleracea var. alboglabra; biosynthesis; genes; gluconapin; oxygenases; plant growth; secondary metabolites;     Show all 8 Subjects
Abstract:
... Glucosinolates are important secondary metabolites in Brassica vegetables, and the side-chain modifications of aliphatic glucosinolates are regulated by 2-oxoglutarate-dependent dioxygenases (AOPs). However, how the side-chain modifications of aliphatic glucosinolates are regulated in Chinese kale remains unclear. Here, we show that the novel gene BoaAOP-like affects the biosynthesis of aliphatic ...
DOI:
10.1016/j.scienta.2022.110921

16. Iron carriers promote biofilm formation and p-nitrophenol degradation

Author:
Lifeng Cao; Ge Zhu; Jinzhao Tao; Yongming Zhang
Source:
Chemosphere 2022 v.293 pp. 133601
ISSN:
0045-6535
Subject:
cytoplasm,   etc   ; Achromobacter; bacterial communities; biochemical pathways; biodiversity; biofilm; iron; p-nitrophenol; sodium acetate;     Show all 9 Subjects
Abstract:
... Vertical baffled biofilm reactors (VBBR) equipped with Plastic-carriers and Fe-carriers were employed to explore the effect of biofilm carriers on biofilm formation and p-nitrophenol (PNP) degradation. The results showed that Fe-carriers enhanced biofilm formation and PNP degradation. The maximum thickness of biofilm grown on the Fe-carriers was 1.5-fold higher than that on the Plastic-carriers. T ...
DOI:
10.1016/j.chemosphere.2022.133601

17. Overexpression of ZmPP2C55 positively enhances tolerance to drought stress in transgenic maize plants

Author:
Pengyu Zhang; Zhen Yuan; Li Wei; Xiao Qiu; Guorui Wang; Zhixue Liu; Jiaxu Fu; Liru Cao; Tongchao Wang
Source:
Plant science 2022 v.314 pp. 111127
ISSN:
0168-9452
Subject:
cytoplasm,   etc   ; corn; drought; electrolyte leakage; genes; proline; serine; stress response; threonine; water stress;     Show all 10 Subjects
Abstract:
... Serine/threonine protein phosphatases play essential roles in plants. PP2C has diverse functions related to development and stress response, while little is known about the functions of PP2C genes with respect to a variety of stresses in maize. In the present study, three ZmPP2C genes, ZmPP2C55, ZmPP2C28, and ZmPP2C71, were identified. Subcellular localization demonstrated that ZmPP2C28 and ZmPP2C ...
DOI:
10.1016/j.plantsci.2021.111127

18. Size and Charge Characterization of Lipid Nanoparticles for mRNA Vaccines

Author:
Camille Malburet; Laurent Leclercq; Jean-François Cotte; Jérôme Thiebaud; Emilie Bazin; Marie Garinot; Hervé Cottet
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4677-4685
ISSN:
1520-6882
Subject:
cytoplasm,   etc   ; analytical chemistry; capillary electrophoresis; electrostatic interactions; immune response; lipids; messenger RNA; vaccines;     Show all 8 Subjects
Abstract:
... Messenger RNA vaccines have come into the spotlight as a promising and adaptive alternative to conventional vaccine approaches. The efficacy of mRNA vaccines relies on the ability of mRNA to reach the cytoplasm of cells, where it can be translated into proteins of interest, allowing it to trigger the immune response. However, unprotected mRNA is unstable and susceptible to degradation by exo- and ...
DOI:
10.1021/acs.analchem.1c04778

19. The disruption of human trophoblast functions by autophagy activation through PI3K/AKT/mTOR pathway induced by exposure to titanium carbide (Ti3C2) MXene

Author:
Limei Yang; Le Hu; Hongyu Tang; Xuemei Chen; Xueqing Liu; Yue Zhang; Yixian Wen; Yongxiu Yang; Yanqing Geng
Source:
Food and chemical toxicology 2022 v.165 pp. 113128
ISSN:
0278-6915
Subject:
cytoplasm,   etc   ; autophagy; cell movement; cell viability; humans; therapeutics; titanium; toxicity; toxicology; trophoblast; villi;     Show all 11 Subjects
Abstract:
... Ti₃C₂ MXene, as a novel nanomaterial, has attracted great attention due to its promising properties in biomedical applications. However, the potential effects of Ti₃C₂ MXene on trophoblast functions have not been investigated. Here, we found that Ti₃C₂ MXene exposure weakened the extension ability of villus explants in vitro. We employed human trophoblast HTR-8/SVneo cells to reveal the underlying ...
DOI:
10.1016/j.fct.2022.113128

20. A role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa

Author:
Béatrice Roche; Mariel A. Garcia‐Rivera; Vincent Normant; Lauriane Kuhn; Philippe Hammann; Mark Brönstrup; Gaëtan L. A. Mislin; Isabelle J. Schalk
Source:
Environmental microbiology 2022 v.24 no.2 pp. 866-877
ISSN:
1462-2912
Subject:
cytoplasm,   etc   ; ABC transporters; Pseudomonas aeruginosa; bacterial growth; bioavailability; iron; molecular biology; opportunistic pathogens; siderophores;     Show all 9 Subjects
Abstract:
... Iron is an essential nutrient for bacterial growth but poorly bioavailable. Bacteria scavenge ferric iron by synthesizing and secreting siderophores, small compounds with a high affinity for iron. Pyochelin (PCH) is one of the two siderophores produced by the opportunistic pathogen Pseudomonas aeruginosa. After capturing a ferric iron molecule, PCH‐Fe is imported back into bacteria first by the ou ...
DOI:
10.1111/1462-2920.15811