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- de Giuseppe, Priscila Oliveira, et al. ; Cordeiro, Rosa Lorizolla; Pirolla, Renan Augusto Siqueira; Persinoti, Gabriela Felix; Gozzo, Fábio Cesar; Murakami, Mario Tyago; Show all 6 Authors
- Journal of molecular biology 2019 v.431 no.4 pp. 732-747
- Bacteroidetes; Bifidobacterium; active sites; bees; beta-mannosidase; carbohydrates; depolymerization; digestive system; ecosystems; hosts; humans; intestinal microorganisms; moieties; nutrition; probiotics; rabbits; swine; symbionts; tryptophan
- ... Bifidobacteria represent one of the first colonizers of human gut microbiota, providing to this ecosystem better health and nutrition. To maintain a mutualistic relationship, they have enzymes to degrade and use complex carbohydrates non-digestible by their hosts. To succeed in the densely populated gut environment, they evolved molecular strategies that remain poorly understood. Herein, we report ...
2. The mechanism by which a distinguishing arabinofuranosidase can cope with internal di-substitutions in arabinoxylans
- de Giuseppe, Priscila Oliveira, et al. ; dos Santos, Camila Ramos; de Souza, Flávio Henrique Moreira; Zanphorlin, Letícia Maria; Domingues, Mariane Noronha; Pirolla, Renan Augusto Siqueira; Honorato, Rodrigo Vargas; Tonoli, Celisa Caldana Costa; de Morais, Mariana Abrahão Bueno; de Matos Martins, Vanesa Peixoto; Fonseca, Lucas Miranda; Büchli, Fernanda; de Oliveira, Paulo Sergio Lopes; Gozzo, Fábio Cesar; Murakami, Mário Tyago; Show all 15 Authors
- Biotechnology for biofuels 2018 v.11 no.1 pp. 223
- alpha-N-arabinofuranosidase; arabinoxylan; biomass; cell walls; corn stover; crystal structure; depolymerization; fungi; grasses; hydrolysis; mass spectrometry; reducing sugars; saccharification; sugarcane; sugarcane bagasse; xylan
- ... BACKGROUND: Arabinoxylan is an abundant polysaccharide in industrially relevant biomasses such as sugarcane, corn stover and grasses. However, the arabinofuranosyl di-substitutions that decorate the xylan backbone are recalcitrant to most known arabinofuranosidases (Abfs). RESULTS: In this work, we identified a novel GH51 Abf (XacAbf51) that forms trimers in solution and can cope efficiently with ...
- PubMed Central:
- de Giuseppe, Priscila Oliveira, et al. ; Vieira, Plínio Salmazo; Souza, Tatiana de Arruda Campos Brasil; Honorato, Rodrigo Vargas; Zanphorlin, Letícia Maria; Severiano, Kelven Ulisses; Rocco, Silvana Aparecida; de Oliveira, Arthur Henrique Cavalcante; Cordeiro, Artur Torres; Oliveira, Paulo Sérgio Lopes; Murakami, Mário Tyago; Show all 11 Authors
- Biochemical and biophysical research communications 2017
- Leishmania major; active sites; amphotericin B; antiparasitic properties; calorimetry; catalytic activity; ligands; molecular models; mutants; new drugs; nucleoside-diphosphate kinase; nucleosides; nucleotides; parasites; site-directed mutagenesis; titration
- ... Nucleoside diphosphate kinases (NDKs) are key enzymes in the purine-salvage pathway of trypanosomatids and have been associated with the maintenance of host-cell integrity for the benefit of the parasite, being potential targets for rational drug discovery and design. The NDK from Leishmania major (LmNDK) and mutants were expressed and purified to homogeneity. Thermal shift assays were employed to ...
4. Structure of a novel class II phospholipase D: Catalytic cleft is modified by a disulphide bridge
- de Giuseppe, Priscila Oliveira, et al. ; Ullah, Anwar; Silva, Dilza Trevisan; Gremski, Luiza Helena; Wille, Ana Carolina Martins; Chaves Moreira, Daniele; Ribeiro, Andrea Senff; Chaim, Olga Meiri; Murakami, Mario Tyago; Veiga, Silvio Sanches; Arni, Raghuvir Krishnaswamy; Show all 11 Authors
- Biochemical and biophysical research communications 2011 v.409 no.4 pp. 622-627
- Loxosceles; Thermus; active sites; crystal structure; disulfide bonds; hemolysis; molecular weight; phospholipase D; phospholipases; point mutation; substrate specificity; venoms
- ... Phospholipases D (PLDs) are principally responsible for the local and systemic effects of Loxosceles envenomation including dermonecrosis and hemolysis. Despite their clinical relevance in loxoscelism, to date, only the SMase I from Loxosceles laeta, a class I member, has been structurally characterized. The crystal structure of a class II member from Loxosceles intermedia venom has been determine ...