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1. Enhanced solid-phase recombinase polymerase amplification and electrochemical detection

Author:
del Río, Jonathan Sabaté,   et al.   ; Lobato, Ivan Magriñà; Mayboroda, Olena; Katakis, Ioanis; O’Sullivan, Ciara K.;     Show all 5 Authors
Source:
Analytical and bioanalytical chemistry 2017 v.409 no.12 pp. 3261-3269
ISSN:
1618-2642
Subject:
DNA; DNA probes; Francisella tularensis; biotin; detection limit; electrochemistry; hares; horseradish; quantitative polymerase chain reaction; temperature
Abstract:
... Recombinase polymerase amplification (RPA) is an elegant method for the rapid, isothermal amplification of nucleic acids. Here, we elucidate the optimal surface chemistry for rapid and efficient solid-phase RPA, which was fine-tuned in order to obtain a maximum signal-to-noise ratio, defining the optimal DNA probe density, probe-to-lateral spacer ratio (1:0, 1:1, 1:10 and 1:100) and length of a ve ...
DOI:
10.1007/s00216-017-0269-y

2. Electrochemical detection of Piscirickettsia salmonis genomic DNA from salmon samples using solid-phase recombinase polymerase amplification

Author:
del Río, Jonathan Sabaté,   et al.   ; Svobodova, Marketa; Bustos, Paulina; Conejeros, Pablo; O’Sullivan, Ciara K.;     Show all 5 Authors
Source:
Analytical and bioanalytical chemistry 2016 v.408 no.30 pp. 8611-8620
ISSN:
1618-2642
Subject:
DNA; Piscirickettsia salmonis; biosensors; detection limit; electrochemistry; electrodes; gold; liver; quantitative polymerase chain reaction; salmon
Abstract:
... Electrochemical detection of solid-phase isothermal recombinase polymerase amplification (RPA) of Piscirickettsia salmonis in salmon genomic DNA is reported. The electrochemical biosensor was constructed by surface functionalization of gold electrodes with a thiolated forward primer specific to the genomic region of interest. Solid-phase RPA and primer elongation were achieved in the presence of t ...
DOI:
10.1007/s00216-016-9639-0

3. Isothermal solid-phase amplification system for detection of Yersinia pestis

Author:
del Rio, Jonathan Sabaté,   et al.   ; Mayboroda, Olena; Benito, Angel Gonzalez; Svobodova, Marketa; Julich, Sandra; Tomaso, Herbert; O’Sullivan, Ciara K.; Katakis, Ioanis;     Show all 8 Authors
Source:
Analytical and bioanalytical chemistry 2016 v.408 no.3 pp. 671-676
ISSN:
1618-2642
Subject:
Yersinia pestis; detection limit; field experimentation; polymerase chain reaction; single-stranded DNA; temperature
Abstract:
... DNA amplification is required for most molecular diagnostic applications, but conventional polymerase chain reaction (PCR) has disadvantages for field testing. Isothermal amplification techniques are being developed to respond to this problem. One of them is the recombinase polymerase amplification (RPA) that operates at isothermal conditions without sacrificing specificity and sensitivity in easy ...
DOI:
10.1007/s00216-015-9177-1

4. Electro-catalytically active Au@Pt nanoparticles for hydrogen evolution reaction: an insight into a tryptophan mediated supramolecular interface towards a universal core–shell synthesis approach

Author:
del Río, Jonathan Sabaté,   et al.   ; Srivastava, Sarvesh Kumar; O'Sullivan, Ciara K.; Ogino, Chiaki; Kondo, Akihiko;     Show all 5 Authors
Source:
RSC advances 2014 v.4 no.89 pp. 48458-48464
ISSN:
2046-2069
Subject:
ambient temperature; catalytic activity; electrochemistry; glassy carbon electrode; gold; hydrogen production; nanoparticles; tryptophan
Abstract:
... We report an eco-friendly, one-pot, room-temperature method for the rapid synthesis of electrocatalytically active Au@Pt (50 nm) bimetallic nanoparticles via a tryptophan (Trp) mediated supramolecular interface in an aqueous environment. Our results demonstrate a simple universal approach for high shell–metal loading where a pre-stabilized tryptophan polymerized-Au core serves as a template to fac ...
DOI:
10.1039/c4ra08365b

5. Rubber-based substrates modified with carbon nanotubes inks to build flexible electrochemical sensors

Author:
del Río, Jonathan Sabaté,   et al.   ; Cuartero, María; Blondeau, Pascal; Ortuño, Joaquín A.; Rius, F. Xavier; Andrade, Francisco J.;     Show all 6 Authors
Source:
Analytica chimica acta 2014 v.827 pp. 95-102
ISSN:
0003-2670
Subject:
analytical chemistry; carbon nanotubes; detection limit; electrochemistry; polymers; potassium; specific ion electrodes; sweat
Abstract:
... The development of a solid-contact potentiometric sensor based on conducting rubbers using a carbon nanotubes ink is described here. Commercial rubbers are turned into conductive ones by a simple and versatile method, i.e. painting an aqueous dispersion of single-walled carbon nanotubes on the polymer surface. On this substrate, both the working ion-selective electrode and the reference electrode ...
DOI:
10.1016/j.aca.2014.04.022

6. Electrochemical detection of Francisella tularensis genomic DNA using solid-phase recombinase polymerase amplification

Author:
del Río, Jonathan Sabaté,   et al.   ; Yehia Adly, Nouran; Acero-Sánchez, Josep Lluis; Henry, Olivier Y.F.; O'Sullivan, Ciara K.;     Show all 5 Authors
Source:
Biosensors & bioelectronics 2014 v.54 pp. 674-678
ISSN:
0956-5663
Subject:
DNA; Francisella tularensis; biosensors; colorimetry; detection limit; electrochemistry; peroxidase; temperature; thiols
Abstract:
... Solid-phase isothermal DNA amplification was performed exploiting the homology protein recombinase A (recA). The system was primarily tested on maleimide activated microtitre plates as a proof-of-concept and later translated to an electrochemical platform. In both cases, forward primer for Francisella tularensis holarctica genomic DNA was surface immobilised via a thiol or an amino moiety and then ...
DOI:
10.1016/j.bios.2013.11.035