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1. Effect of heat treatment on the conformational stability of intact and cleaved forms of the peanut allergen Ara h 6 in relation to its IgE-binding potency

Author:
de Jongh, Harmen H.J.; de Jong, Govardus A.H.; Apostolovic, Danijela; Taylor, Steve L.; Baumert, Joseph L; Koppelman, Stef J.
Source:
Food chemistry 2020 v.326 pp. 127027
ISSN:
0308-8146
Subject:
denaturation,   etc   ; activation energy; allergens; heat treatment; peanuts;     Show all 5 Subjects
Abstract:
... This work reports on theeffect of heat treatment on the protein conformational stabilityof intact and post-translationallycleaved peanut allergen Ara h 6 in relation to IgE-binding. Intact and post-translationallycleaved Ara h 6 are structurally similar and theirstrong resistance to denaturant-inducedunfolding is comparable. Only upon exposure toautoclave conditions the twoforms of Ara h 6 demonst ...
DOI:
10.1016/j.foodchem.2020.127027

2. Influence of ohmic heating on commercial peroxidase and sugarcane juice peroxidase inactivation

Author:
Brochier, Bethania; Hertz, Plinho Francisco; Marczak, Ligia Damasceno Ferreira; Mercali, Giovana Domeneghini
Source:
Journal of food engineering 2020 v.284 pp. 110066
ISSN:
0260-8774
Subject:
denaturation,   etc   ; electric field; isozymes; ohmic heating; peroxidase; sucrose; sugarcane juice;     Show all 7 Subjects
Abstract:
... Different fractions of sugarcane juice (liquid, solid and concentrated) were ohmically (75 °C and 7.8 V/cm for 25 min) treated to investigate the effect of different sample compounds on peroxidase inactivation. Moreover, commercial peroxidase solutions with and without the addition of sucrose were evaluated during ohmic (OH) and conventional heating (CH – with the same parameters of OH but without ...
DOI:
10.1016/j.jfoodeng.2020.110066

3. The conformational stability of terminal helices of [formula omitted]-repressor protein in aqueous dodine and choline-O-sulfate solutions

Author:
Paul, Srijita; Paul, Sandip
Source:
International journal of biological macromolecules 2020 v.154 pp. 1332-1346
ISSN:
0141-8130
Subject:
denaturation,   etc   ; acetates; dodine; ions; molecular dynamics; simulation models;     Show all 6 Subjects
Abstract:
... In this article, we have ventured into the denaturation of fast folding λ6-85-repressor protein at a millimolar concentration of dodine and henceforth, evaluated the candidature of choline-O-sulfate as a protecting osmolyte against it, employing classical molecular dynamics simulations. Our simulation results show that, the terminal helices of λ-repressor protein get unfolded in presence of ∼15 mM ...
DOI:
10.1016/j.ijbiomac.2019.11.013

4. Water dynamics changes and protein denaturation in surf clam evaluated by two-dimensional LF-NMR T1-T2 relaxation technique during heating process

Author:
Wang, Siqi; Lin, Rong; Cheng, shasha; Tan, Mingqian
Source:
Food chemistry 2020 v.320 pp. 126622
ISSN:
0308-8146
Subject:
denaturation; protein denaturation,   etc   ; Mactridae; chewiness; clams; color; differential scanning calorimetry; hardness; heat; magnetic resonance imaging; microstructure; nuclear magnetic resonance spectroscopy; temperature; water holding capacity;     Show all 14 Subjects
Abstract:
... Water dynamics and protein denaturation in surf clam during heating were studied by the two-dimensional low-field nuclear magnetic resonance (LF-NMR) T₁-T₂ relaxation technique. A significant change was found for clam around 80 °C and direct visualization of the water state change was provided by the magnetic resonance imaging. Principal components and heatmap analysis revealed that clam treated a ...
DOI:
10.1016/j.foodchem.2020.126622

5. Estimating the structure of sarcoplasmic proteins extracted from pork tenderloin thawed by a high-voltage electrostatic field

Author:
Jia, Guoliang; van den Berg, Frans; Hao, Han; Liu, Haijie
Source:
Journal of food science and technology 2020 v.57 no.4 pp. 1574-1578
ISSN:
0022-1155
Subject:
denaturation; protein denaturation,   etc   ; Fourier transform infrared spectroscopy; differential scanning calorimetry; polyacrylamide gel electrophoresis; pork; principal component analysis; protein solubility; proteins; thawing;     Show all 10 Subjects
Abstract:
... The application of high-voltage electrostatic field (HVEF) is a novel method of thawing. To determine if HVEF thawing could lead to sarcoplasmic proteins denaturation, and to provide a theoretical estimation of the structure of the sarcoplasmic proteins, pork tenderloin was thawed by traditional and HVEF methods. The results from protein solubility analysis, sodium dodecyl sulfate–polyacrylamide g ...
DOI:
10.1007/s13197-020-04253-4
PubMed:
32180654
PubMed Central:
PMC7054592

6. Bioactive proteins in bovine colostrum and effects of heating, drying and irradiation

Author:
Chatterton, Dereck E. W.; Aagaard, Sasha; Hesselballe Hansen, Tim; Nguyen, Duc Ninh; De Gobba, Cristian; Lametsch, René; Sangild, Per T.
Source:
Food & function 2020 v.11 no.3 pp. 2309-2327
ISSN:
2042-650X
Subject:
denaturation; protein denaturation,   etc   ; cow colostrum; gamma radiation; heat; immunoglobulin G; irradiation; lactalbumin; lactoferrin; methionine; microbial contamination; pasteurization; patients; peroxidase; reactive oxygen species; spray drying;     Show all 16 Subjects
Abstract:
... Bovine colostrum (BC) contains bioactive proteins, such as immunoglobulin G (IgG), lactoferrin (LF) and lactoperoxidase (LP). BC was subjected to low-temperature, long-time pasteurization (LTLT, 63 °C, 30 min) or high-temperature, short-time pasteurization (HTST, 72 °C, 15 s) and spray-drying (SD), with or without γ-irradiation (GI, ∼14 kGy) to remove microbial contamination. Relative to unpasteur ...
DOI:
10.1039/c9fo02998b

7. Probing Protein Denaturation during Size-Exclusion Chromatography Using Native Mass Spectrometry

Author:
Ventouri, Iro K.; Malheiro, Daniel B. A.; Voeten, Robert L. C.; Kok, Sander; Honing, Maarten; Somsen, Govert W.; Haselberg, Rob
Source:
Analytical chemistry 2020 v.92 no.6 pp. 4292-4300
ISSN:
1520-6882
Subject:
denaturation; protein denaturation,   etc   ; additives; ammonium; ammonium acetate; bicarbonates; carbonate dehydratase; cytochrome c; electrospray ionization mass spectrometry; formates; gel chromatography; ionic strength; models; myoglobin; pH;     Show all 15 Subjects
Abstract:
... Size-exclusion chromatography employing aqueous mobile phases with volatile salts at neutral pH combined with electrospray-ionization mass spectrometry (SEC-ESI-MS) is a useful tool to study proteins in their native state. However, whether the applied eluent conditions actually prevent protein–stationary phase interactions, and/or protein denaturation, often is not assessed. In this study, the eff ...
DOI:
10.1021/acs.analchem.9b04961
PubMed:
32107919
PubMed Central:
PMC7081181

8. A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics

Author:
Kirley, Terence L.; Norman, Andrew B.; Wetzel, Hanna N.
Source:
Journal of immunological methods 2020 v.476 pp. 112676
ISSN:
0022-1759
Subject:
denaturation; protein denaturation,   etc   ; binding capacity; clinical trials; cocaine; fluorescent dyes; fluorometry; hydrophobicity; ligands; metabolites; monoclonal antibodies; protein domains; quantitative analysis; therapeutics; thermal stability;     Show all 15 Subjects
Abstract:
... An anti-cocaine monoclonal antibody (mAb) designated h2E2 will soon enter clinical trials for the treatment of cocaine abuse disorders. Importantly, this antibody selectively binds cocaine and its active metabolite, cocaethylene, with high affinity, while binding inactive metabolites with substantially lower affinities. Here, we used differential scanning fluorimetry (DSF) to characterize the stab ...
DOI:
10.1016/j.jim.2019.112676
PubMed:
31634480
PubMed Central:
PMC6939126

9. The threshold of amyloid aggregation of beta-lactoglobulin: Relevant factor combinations

Author:
Heyn, Timon R.; Mayer, Julian; Neumann, Hendrikje R.; Selhuber-Unkel, Christine; Kwade, Arno; Schwarz, Karin; Keppler, Julia K.
Source:
Journal of food engineering 2020 v.283 pp. 110005
ISSN:
0260-8774
Subject:
protein denaturation,   etc   ; amyloid; beta-lactoglobulin; developmental stages; mixing; pH; shear stress; temperature;     Show all 8 Subjects
Abstract:
... This study identifies critical factor combinations of pH, temperature, stirring speed, protein and ion concentration that specifically affect the lag-, and growth phase of beta-lactoglobulin amyloid aggregation and provides information on how, when and why certain factor combinations affect the onset of amyloid aggregation.Conditions at the threshold of amyloid formation were chosen to prolong the ...
DOI:
10.1016/j.jfoodeng.2020.110005

10. Development of a direct and visual isothermal method for meat adulteration detection in low resource settings

Author:
Yan, Chunyu; Wang, Xiudan; Zhao, Xiaoli; Wei, Manman; Shi, Chao; Ma, Cuiping
Source:
Food chemistry 2020 v.319 pp. 126542
ISSN:
0308-8146
Subject:
denaturation,   etc   ; DNA; adulterated products; color; duck meat; ducks; heat; mitochondria; point-of-care systems;     Show all 9 Subjects
Abstract:
... Simple, fast and efficient method for on-site detection of meat adulteration is of increasing demand in low-resource regions. Here, a direct and visual denaturation bubble-mediated strand exchange amplification method (SEA) was developed to detect meat adulteration by targeting the mitochondrial sequence from duck, which required only a heating block. The method allowed detecting as low as 10 pg/μ ...
DOI:
10.1016/j.foodchem.2020.126542

11. The sol-gel-sol transformation behavior of egg white proteins induced by alkali

Author:
Gao, Xuejing; Yao, Yao; Wu, Na; Xu, Mingsheng; Zhao, Yan; Tu, Yonggang
Source:
International journal of biological macromolecules 2020 v.155 pp. 588-597
ISSN:
0141-8130
Subject:
denaturation,   etc   ; Fourier transform infrared spectroscopy; alkali treatment; alkalinity; egg albumen; gels; hardness; hydrophobicity; liquefaction; peptides; viscosity;     Show all 11 Subjects
Abstract:
... In the current study, we found an interesting phenomenon that fresh egg white (EW) undergo the sol-gel-sol transition with alkali treatment. The transformation behavior at different alkalinity (1.5%, 2.0%, and 2.5%) was investigated. As the gel formed, the hardness, lightness, surface hydrophobicity and the total number of identified peptides increased, and then, remarkable reduction when the gel ...
DOI:
10.1016/j.ijbiomac.2020.03.209

12. Reactive oxygen species and their applications toward enhanced lipid accumulation in oleaginous microorganisms

Author:
Zhang, Sai; He, Yaodong; Sen, Biswarup; Wang, Guangyi
Source:
Bioresource technology 2020 v.307 pp. 123234
ISSN:
0960-8524
Subject:
protein denaturation,   etc   ; DNA damage; antioxidant activity; biofuels; byproducts; lipid peroxidation; lipids; microorganisms; reactive oxygen species;     Show all 9 Subjects
Abstract:
... Oleaginous microorganisms are among the most promising alternative sources of lipids for oleochemicals and biofuels. However, in the course of lipid production, reactive oxygen species (ROS) are generated inevitably as byproducts of aerobic metabolisms. Although excessive accumulation of ROS leads to lipid peroxidation, DNA damage, and protein denaturation, ROS accumulation has been suggested to e ...
DOI:
10.1016/j.biortech.2020.123234

13. Kinetics of denaturation and aggregation of highly concentrated β-Lactoglobulin under defined thermomechanical treatment

Author:
Quevedo, Maria; Kulozik, Ulrich; Karbstein, Heike P.; Emin, M. Azad
Source:
Journal of food engineering 2020 v.274 pp. 109825
ISSN:
0260-8774
Subject:
denaturation,   etc   ; activation energy; beta-lactoglobulin; chemical bonding; heat; mechanical methods; temperature;     Show all 7 Subjects
Abstract:
... The influence of thermomechanical treatment (temperature 60 °C – 100 °C and shear rate 0.05 s⁻¹ – 50 s⁻¹) on the denaturation and aggregation of β-Lactoglobulin at high concentrations (50 % – 70 %) was investigated. While denaturation and aggregation reaction rate decreased at higher concentrations, a combination of heat and mechanical treatment resulted in an increase in reaction rates and conseq ...
DOI:
10.1016/j.jfoodeng.2019.109825

14. Denaturant effect on amyloid fibrils: Declasterization, depolymerization, denaturation and reassembly

Author:
Sulatsky, M.I.; Sulatskaya, A.I.; Stepanenko, Olga V.; Povarova, O.I.; Kuznetsova, I.M.; Turoverov, K.K.
Source:
International journal of biological macromolecules 2020 v.150 pp. 681-694
ISSN:
0141-8130
Subject:
denaturation,   etc   ; acute kidney injury; amyloid; amyloidosis; depolymerization; hemodialysis; microdialysis; models; pathogenicity; protein aggregates;     Show all 10 Subjects
Abstract:
... Accumulation of amyloid fibrils in organism accompanies many serious diseases, such as Alzheimer's and Parkinson's diseases, diabetes, prion diseases, etc. It is generally accepted that amyloids are highly resistant to degradation, which complicates their elimination in vivo and is one of the reasons for their pathogenicity. However, using a wide range of physicochemical approaches and specially e ...
DOI:
10.1016/j.ijbiomac.2020.01.290

15. Molecular and macromolecular crowding-induced stabilization of proteins: Effect of dextran and its building block alone and their mixtures on stability and structure of lysozyme

Author:
Ghosh, Shayamita; Shahid, Sumra; Raina, Neha; Ahmad, Faizan; Hassan, Md. Imtaiyaz; Islam, Asimul
Source:
International journal of biological macromolecules 2020 v.150 pp. 1238-1248
ISSN:
0141-8130
Subject:
denaturation,   etc   ; Gibbs free energy; dextran; enthalpy; entropy; glucose; lysozyme; proteins; solutes;     Show all 9 Subjects
Abstract:
... Dextran 70 and its building block (glucose) has been used as macromolecular crowder and osmolyte, respectively. The difference in size and structure of both made us inquisitive to measure stability of lysozyme in the presence of their mixture. The effects of mixture of a fixed dextran 70 concentration (300 mg/ml) containing different concentrations of glucose and vice versa, were studied. It was o ...
DOI:
10.1016/j.ijbiomac.2019.10.135

16. Effects of the thermal denaturation of Sabin-derived inactivated polio vaccines on the D-antigenicity and the immunogenicity in rats

Author:
Murakami, Kosuke; Fujii, Yoshiki; Someya, Yuichi
Source:
Vaccine 2020 v.38 no.17 pp. 3295-3299
ISSN:
0264-410X
Subject:
denaturation,   etc   ; heat treatment; immunogenicity; neutralizing antibodies; quality control; rats; vaccines; Japan;     Show all 8 Subjects
Abstract:
... The efficacy of a Sabin-derived inactivated polio vaccine (sIPV) can be evaluated by measuring the immunogenicity and the contents of D-antigens, which induce the neutralizing antibodies. The immunogenic potency test in rats was done as a national assay in Japan. The two manufacturers of sIPV in Japan have performed both assays since development, and there is no clear discrepancy between the resul ...
DOI:
10.1016/j.vaccine.2020.03.027

17. Effects of phosphate ion concentration on in-vitro fibrillogenesis of sturgeon type I collagen

Author:
Meng, Dawei; Li, Wen; Ura, Kazuhiro; Takagi, Yasuaki
Source:
International journal of biological macromolecules 2020 v.148 pp. 182-191
ISSN:
0141-8130
Subject:
denaturation,   etc   ; cell culture; cell proliferation; collagen; fibroblasts; phosphates; sturgeon; swim bladder; temperature; thermal stability; tissue engineering;     Show all 11 Subjects
Abstract:
... Nonmammalian collagens have attracted significant attention owing to their potential for use as a source of cell scaffolds for tissue engineering. Since the morphology of collagen fibrils controls cell proliferation and differentiation, its regulation is essential for fabricating scaffolds with desirable characteristics. In this study, we evaluated the effects of the phosphate ion (Pi) concentrati ...
DOI:
10.1016/j.ijbiomac.2020.01.128

18. Whey protein-polysaccharide conjugates obtained via dry heat treatment to improve the heat stability of whey protein stabilized emulsions

Author:
Setiowati, Arima Diah; Wijaya, Wahyu; Van der Meeren, Paul
Source:
Trends in food science & technology 2020 v.98 pp. 150-161
ISSN:
0924-2244
Subject:
protein denaturation,   etc   ; emulsifiers; emulsions; food industry; heat; heat stability; heat treatment; polysaccharides; temperature; whey; whey protein;     Show all 11 Subjects
Abstract:
... As the demand for “clean label emulsions” and natural emulsifiers is increasing, whey proteins have a big potency to be used as an emulsifier in food emulsions. However, in order to enable their application, whey proteins should withstand high temperature processing. Hence, the limited heat stability of whey proteins is a major drawback: they are highly heat labile and thus prone to heat induced p ...
DOI:
10.1016/j.tifs.2020.02.011

19. Novel noscapine derivatives stabilize the native state of insulin against fibrillation

Author:
Alijanvand, Saeid Hadi; Christensen, Mikkel Hovden; Christiansen, Gunna; Harikandei, Kosar Babanezhad; Salehi, Peyman; Schiøtt, Birgit; Moosavi-Movahedi, Ali Akbar; Otzen, Daniel E.
Source:
International journal of biological macromolecules 2020 v.147 pp. 98-108
ISSN:
0141-8130
Subject:
denaturation,   etc   ; Papaver somniferum; amyloid; cell viability; human diseases; humans; insulin; models; toxicity;     Show all 9 Subjects
Abstract:
... Protein aggregation to form amyloid is associated with many human diseases, increasing the need to develop inhibitors of this process. Here we evaluate the ability of derivatives of the small organic compound noscapine, derived from the opium poppy, to inhibit fibrillation of the model protein insulin. We combined biophysical methods to assess insulin stability and aggregation with computational d ...
DOI:
10.1016/j.ijbiomac.2020.01.061

20. Artificial peptide-controlled protein release of Zn2+-triggered, self-assembled histidine-tagged protein microparticle

Author:
Chen, Tzu-Yin; Cheng, Wan-Jung; Horng, Jia-Cherng; Hsu, Hsin-Yun
Source:
Colloids and surfaces 2020 v.187 pp. 110644
ISSN:
0927-7765
Subject:
protein denaturation,   etc   ; drug delivery systems; drugs; microparticles; peptides; recombinant proteins; zinc;     Show all 7 Subjects
Abstract:
... Protein microparticles have received attention as drug delivery systems because of their high protein stability and prolonged release in vivo. However, most current preparation processes introduce chemical crosslinkers, which often lead to protein inactivation and limit drug efficacy in delivery systems. In this study, we employed the well-known hexahistidine (His)-tag recombinant protein technolo ...
DOI:
10.1016/j.colsurfb.2019.110644