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Valerij Y. Grinberg; Tatiana V. Burova; Natalia V. Grinberg; Vladimir E. Tikhonov; Alexander S. Dubovik; Victor N. Orlov; Irina G. Plashchina; Anatolii I. Usov; Alexei R. Khokhlov
denaturation, etc ; chitosan; enthalpy; entropy; hydrocolloids; pH; sorption isotherms; temperature; Show all 8 Subjects
Abstract:
... β-lactoglobulin–oligochitosan (MW 9500 Da) system was studied under conditions of protein-polysaccharide incompatibility, the dipole-charge, and charge-charge protein-polysaccharide interactions at pH 3.0, 5.5, and 6.0, respectively, using ITC, DSC, and DLS. At pH 5.5 and 6.0, the ITC data revealed a complex formation between β-lactoglobulin and oligochitosan. The binding curves of the protein to ...
... Trans-2-hexenal (T2H) has great commercial value for development as a biopesticide, but its toxicity risk to nontarget organisms is unknown. Here, the toxicity and underlying mechanism of T2H on zebrafish (Danio rerio) were investigated. The LC₅₀ (48 h) of T2H on zebrafish is 4.316 μg/mL, and the aldehyde group is essential to its toxicity. In 14-day chronic toxicity tests, 0.432 μg/mL T2H resulte ...
denaturation, etc ; Setaria italica; glutelins; hydrocolloids; hydrophobicity; particle size; prolamins; protein content; protein sources; solubility; ultrasonics; zeta potential; Show all 12 Subjects
Abstract:
... Foxtail millet protein constitutes the second most important component and has remarkable essential acid composition. Out of which prolamin and glutelin forms the major portion of this protein. In the present study, foxtail millet protein was obtained with a recovery yield of 70.79%, and prolamin and glutelin fractions constituted 31.10 and 34.69% of total protein. The objective of this study was ...
... Aflatoxin as the potent carcinogenic mycotoxin had been received great attention for detection in food industry and safety. Due to low quantity of aflatoxin in food samples, there is a need to develop a sensitive method toward its detection. In the present study, an aflatoxin B1 (AFB1) specific aptasensor with internal complementary sequence was developed for detection of AFB1. The FAM-functionali ...
... Single molecule force-spectroscopy techniques have granted access to unprecedented molecular-scale details about biochemical and biological mechanisms. However, the interpretation of the experimental data is often challenging. Computational and simulation approaches (all-atom steered MD simulations in particular) are key to provide molecular details about the associated mechanisms, to help test di ...
denaturation; protein denaturation, etc ; bovine serum albumin; cattle; chemical bonding; hydrocolloids; immunoglobulin G; temperature; Show all 8 Subjects
Abstract:
... Shearing can modify native properties of proteins alone or in combination with heating. Effects of shearing on secondary structure, extent of denaturation and interactions of immunoglobulin G (IgG) and bovine serum albumin (BSA) were studied at temperatures where these changes start to take place. Pure IgG (0.8 mL mL⁻¹) or BSA (0.4 mg mL⁻¹) and their binary mixture were subjected to selected tempe ...
denaturation; protein denaturation, etc ; gelation; heat treatment; milk fat; pH; recombined milk; skim milk; whey; whey protein; whole milk; Show all 11 Subjects
Abstract:
... Recombined whole milk was prepared by heating skim milk before homogenising with milkfat (HEHO milk) or by homogenising milkfat with unheated skim milk and heating the recombined milk (HOHE milk). Heat treatment caused whey protein denaturation, more at higher temperatures and slightly more in HEHO milk than in HOHE milk. Less protein was adsorbed to the fat globules of the HEHO milk than the HOHE ...
denaturation; protein denaturation, etc ; crystallization; freeze-thaw cycles; ice; ionic strength; meat; meat science; muscles; pH; water holding capacity; Show all 11 Subjects
Abstract:
... Formation of thaw loss cannot generally be avoided when meat is frozen and then thawed. Explanations have mainly focused on the damage to muscle fibers resulting from ice crystallization and the freezing-induced denaturation of myofibrillar proteins, the latter of which has, however, not received much research focus. This review discusses the relationship between myofibrillar protein denaturation ...
denaturation; protein denaturation, etc ; combustion; droplet size; droplets; drugs; energy; kinetics; mathematical models; product quality; temperature; thermosensitivity; whey protein isolate; Show all 13 Subjects
Abstract:
... Pulse combustion drying is a novel advanced drying technique that uses pulsating gas flow generated by intermittent combustion. The technique has been used for fine powder production from various materials, including heat sensitive materials such as foods and pharmaceuticals. In this study, denaturation of whey protein isolate (WPI) during drying in pulsated gas flow is investigated numerically. A ...
denaturation; protein denaturation, etc ; bound water; fatty acids; humans; hydrogen; intestines; magnetism; models; molecular dynamics; nuclear magnetic resonance spectroscopy; urea; Show all 12 Subjects
Abstract:
... The mechanism of urea's action in protein denaturation remains largely unknown. To provide an experimental basis for molecular dynamics (MD) simulations on urea-protein interactions, we investigated the effect of urea on human intestinal fatty acid binding protein (hIFABP) by nuclear magnetic resonance (NMR). Hydrogen-deuterium exchange (HDX) rates at ≤ 2 M urea indicate that urea affects hIFABP i ...
denaturation; protein denaturation, etc ; X-ray diffraction; cellobiose; cryoprotectants; crystallization; food chemistry; freeze-thaw cycles; hydrophobicity; pH; particle size; protein degradation; Show all 12 Subjects
Abstract:
... The aim of this study was to investigate myofibrillar protein (MFP) denaturation induced by pH changes during freeze–thaw (FT) cycles, and to propose an effective mitigation strategy. Owing to the selective crystallization of Na₂HPO₄·12H₂O and the consequent pH change, a pH change of 3.32 units was observed when the MFP solution were frozen. The surface hydrophobicity, particle size and confocal l ...
denaturation, etc ; corn; food chemistry; hydrophobicity; zearalenone; zein; Show all 6 Subjects
Abstract:
... Zein, the plant protein, has received great attention in the last years. However, hidden zearalenone (ZEN), the zein-bound ZEN present in zein would have a great hazard to humans and animals. To date, the fates of hidden ZEN under different extraction methods of zein have not been clarified. In this study, the effect of zein extraction methods on conversion of free ZEN with hidden ZEN and its corr ...
denaturation, etc ; antioxidants; cattle; collagen; food industry; gelatin; swine; Show all 7 Subjects
Abstract:
... Gelatin, which is widely used in the food industry, is a protein derived from partial denaturation of collagen. The primary sources of industrial gelatin are porcine skin or bovine hide, but their utilization is restricted due to disease outbreaks and on religious grounds. Therefore, gelatin derived from aquatic organisms is attracting widespread interest and can be a good source for harvesting of ...
denaturation, etc ; cattle; enthalpy; entropy; meat; meat science; temperature; Show all 7 Subjects
Abstract:
... Thermal properties of muscles are of great interest in meat science. A recent article (Vaskoska et al., 2021) examines the thermal denaturation of proteins in bovine muscles, masseter and cutaneous trunci, by measuring the enthalpy and melting temperature of the denaturation. In the present article, we report the numerical values of entropy in the denaturation of proteins. Furthermore, we describe ...
denaturation, etc ; DNA; energy; heat; irradiation; molecular dynamics; nanogold; Show all 7 Subjects
Abstract:
... There is significant interest in developing photothermal systems that can precisely control the structure and function of biomolecules through local temperature modulation. One specific application is the denaturation of double-stranded (ds) DNA through femtosecond (fs) laser pulse optical heating of gold nanoparticles (AuNPs); however, the mechanism of DNA melting in these systems is not fully un ...
protein denaturation, etc ; Maillard reaction; food chemistry; food industry; Show all 4 Subjects
Abstract:
... In modern days, to meet the steadily growing demand for novel and nutritional food products several innovative technologies have been used in the food industry. Among them, explosion puffing (EP) is considered an efficient method for the processing of food products and is proposed as an alternative to conventional processing methods. This review discusses the wide applications of EP in drying, puf ...
denaturation; protein denaturation, etc ; amino acids; dialysis; digestion; heat; hydrolysis; infant formulas; ingredients; intermediate product; models; peptides; protein composition; protein depletion; whey protein isolate; Show all 15 Subjects
Abstract:
... Knowledge about how molecular properties of proteins affect their digestion kinetics is crucial to understand protein postprandial plasma amino acid (AA) responses. Previously it was found that a native whey protein isolate (NWPI) and heat denatured whey protein isolate (DWPI) elicit comparable postprandial plasma AA peak concentrations in neonatal piglets, while a protein base ingredient for infa ...
denaturation, etc ; biotechnology; food science; heat; modified starch; value added; Show all 6 Subjects
Abstract:
... Starch is often subjected to denaturation treatment to improve its useful properties and eliminate its shortcomings. Various methods have been developed to produce modified starches with different properties and for a variety of uses. Because physically modified starch can be produced without chemical substances or biological agents, the modification method is very simple and inexpensive, and the ...
denaturation; protein denaturation, etc ; Sargassum; acarbose; acetone; albumins; alpha-amylase; anti-inflammatory activity; antioxidants; coasts; ethyl acetate; hexane; ketoprofen; phlorotannins; sulfates; triacylglycerol lipase; Mediterranean Sea; Show all 17 Subjects
Abstract:
... Brown seaweeds are a good source of bioactive compounds, particularly of phlorotannins, which may exert a wide spectrum of pharmacological properties. In the present study, phlorotannins of S. vulgare were extracted using a 70% acetone solution and the crude extract was further purified through liquid–liquid partition, giving rise to n-hexane, ethyl acetate and aqueous residue fractions. The crude ...
denaturation, etc ; cytosol; endoplasmic reticulum; heat stress; hydrolysis; molecular biology; Show all 6 Subjects
Abstract:
... Hsp90 and Hsp70 are highly conserved molecular chaperones that promote the proper folding and activation of substrate proteins that are often referred to as clients. The two chaperones functionally collaborate to fold specific clients in an ATP-dependent manner. In eukaryotic cytosol, initial client folding is done by Hsp70 and its co-chaperones, followed by a direct transfer of client refolding i ...