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diacylglycerols, etc ; egg yolk; food chemistry; glycerophospholipids; lipidomics; lysophospholipids; metabolism; metabolites; sphingolipids; sterols; storage time; Show all 11 Subjects
Abstract:
... A comprehensive UPLC-MS/MS-based lipidomics analysis of egg yolk at different storage periods was performed. Through the analysis, 836 lipid species from 27 subclasses were detected, including 159 significantly different lipid species. Glycerolipids (GL) and glycerophospholipids (GP) were the most abundant lipids, followed by fatty acyls (FA), sphingolipids (SP), sterol lipids (ST), and prenol lip ...
diacylglycerols, etc ; Pampus argenteus; Thunnus obesus; food composition; marine fish; mass spectrometry; palmitoleic acid; phospholipids; sardines; seafoods; ultra-performance liquid chromatography; Show all 11 Subjects
Abstract:
... Palmitoleic acid (POA) is a monounsaturated n-7 fatty acid (16:1 n-7) that is active in modulating cardiovascular and metabolic processes. POA-structured phospholipids, diglycerides, and triglycerides (POA-PL, -DG, -TG) are abundantly present in marine life. In this study, POA and POA-lipids contained in crude lipids in visceral by-products of six seafood products (n = 6 for each sample) were anal ...
diacylglycerols, etc ; chickens; docosahexaenoic acid; egg yolk; eicosapentaenoic acid; food composition; lipid composition; lipidomics; liquid chromatography; lysophosphatidylcholine; phosphatidylethanolamines; tandem mass spectrometry; Show all 12 Subjects
Abstract:
... Chicken egg yolk (EY) can be divided into yolk granule (EYG) and yolk plasma (EYP); they develop individually based on their remarkably different nutritional compositions and properties. However, the differences in lipid profiles between EYG and EYP have not been fully revealed. In this study, quantitative lipidomic analyses of EY, EYG, and EYP were implemented through a liquid chromatography-tand ...
diacylglycerols, etc ; flavor; food chemistry; intramuscular fat; lipid peroxidation; lipidomics; odors; Show all 7 Subjects
Abstract:
... Intramuscular fat (IMF) content is associated with lamb flavor and largely varies between individuals of the same breed. However, studies investigating the effects of IMF variations on lipid profiles and aroma compounds are limited. Here, we performed lipidomic and volatilomic profiling of high-IMF and low-IMF Hu lambs. Triglycerides and diglycerides in the high-IMF group were significantly higher ...
diacylglycerols, etc ; Saanen; food research; glucosylceramides; goat milk; lipidomics; sphingomyelins; traceability; triacylglycerols; China; Show all 10 Subjects
Abstract:
... The lipids in goat milk from Guangdong Province (GGM), Shaanxi Province (SGM), and Inner Mongolia Province (NGM) were analyzed and compared using untargeted lipidomics. A total of 16 lipid sub-classes and 638 lipid molecules were identified in the three groups. The main lipids were diacylglycerol (DG), triacylglycerol (TG), and glycerophosphatidylethanolamine (PE). The contents of glycerophosphati ...
diacylglycerols, etc ; lipidomics; lysophosphatidylcholine; mass spectrometry; positional isomers; supercritical fluid chromatography; temperature; triacylglycerols; Show all 8 Subjects
Abstract:
... Powerful chromatographic techniques are required for lipidomic analyses due to the extreme complexity of natural lipidomes. In the past few years, ultrahigh-performance supercritical fluid chromatography (UHPSFC) has proven to be a good alternative to conventional LC methods for comprehensive lipidomic analysis. The goal of this work was to study UHPSFC intraclass separation of lipids according to ...
diacylglycerols, etc ; Marinobacter aquaeolei; acyl coenzyme A; biodiesel; biosynthesis; carbon; catalytic activity; crystal structure; Show all 8 Subjects
Abstract:
... The biosynthesis of wax esters and triglycerides in bacteria is accomplished through the action of the wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase (WS/DGAT or wax ester synthase). A hallmark of these enzymes is the broad substrate profile that accepts alcohols, diglycerides, and fatty acyl-CoAs of various carbon chain lengths and degrees of branching. These enzymes have a bro ...
diacylglycerols, etc ; biosynthesis; brain; cannabinoids; carboxylic ester hydrolases; fluorescence; humans; liver; microsomes; rats; spermatozoa; Show all 11 Subjects
Abstract:
... 1,2-Diacylglycerol lipases (DAGLs) are the most important enzymes for the biosynthesis of the endocannabinoid 2-arachidonoylglycerol (2-AG), and their role in various pathophysiological conditions is currently under investigation. We synthesized a new 1,2-diacylglycerol substrate for these enzymes with a fluorogenic 4-(pyren-1-yl)butanoyl residue in sn-2 position. Using the fluorescent substrate, ...
diacylglycerols, etc ; fermentation; grapes; lipid content; lipidomics; mouthfeel; phospholipids; sweetness; taste; temperature; winemaking; wines; yeasts; Show all 13 Subjects
Abstract:
... Lipids have important impacts on wine sensory. By targeting the lipid sources in wine, mainly from grape tissues and yeast cell walls, it was possible to alter the wine lipid profile thus potentially changing the final product quality. This research examined the changes of wine total lipids, lipid composition and sensory characteristics of Pinot noir wines in response to the winemaking factors, fe ...
diacylglycerols, etc ; Trichiurus; food chemistry; frozen storage; hydrolysis; lipidomics; muscles; oxidation; phosphatidylcholines; phosphatidylethanolamines; storage time; Show all 11 Subjects
Abstract:
... Chemical and LC/MS-based lipidomics strategies were performed to explore the alterations of lipid profiles in hairtail (Trichiurus haumela) muscle during 120 days of frozen storage. Chemical results indicated that the PV and TBARS values in hairtail muscle significantly increased during 120 days of frozen storage. Lipidomics results detected 1223 lipids in hairtail muscle assigned to 26 lipid cate ...
diacylglycerols, etc ; Candida; bioreactors; carboxylic ester hydrolases; esterification; glycerol; hydrodynamics; mass transfer; models; oleic acid; Show all 10 Subjects
Abstract:
... Diacylglycerols (DAG) have been widely used in many industries due to their remarkable capabilities as emulsifiers and stabilisers. However, developing a sustainable and an effective synthesis method for DAG remains a challenge. Continuous flow bio-reactor is recognized to be more productive, controllable, and reliable instrument for developing green and intensified processes. In this work, a cont ...
diacylglycerols, etc ; Bacillus cereus; enzyme activity; hydrolysis; lipolysis; mammals; phosphatidylcholines; phospholipase C; phospholipases; phosphorylcholine; spectroscopy; Show all 11 Subjects
Abstract:
... Phospholipase C (PLC) represents an important type of enzymes with the feature of hydrolyzing phospholipids at the position of the glycerophosphate bond, among which PLC extracted from Bacillus cereus (BC-PLC) has been extensively studied owing to its similarity to hitherto poorly characterized mammalian analogues. This study focuses on investigating the interfacial hydrolysis mechanism of phospha ...
diacylglycerols, etc ; acid phosphatase; cytoplasm; immunocytochemistry; inositols; mammals; phosphatidylcholines; phospholipase C; phospholipases; plasma membrane; research; Show all 11 Subjects
Abstract:
... Activation of Gq protein-coupled receptors triggers the phospholipase C (PLC) pathway, which yields a pair of second messengers: diacylglycerol (DG) and inositol 1,4,5-trisphosphate (IP₃). DG kinase (DGK) phosphorylates DG to produce phosphatidic acid (PA), which serves as another second messenger. Along with PLC-DGK pathway, PA is produced directly by the action of phospholipase D (PLD), which hy ...
diacylglycerols, etc ; Sardina pilchardus; freshness; ice; polyunsaturated fatty acids; raw materials; storage time; texture; triacylglycerols; Show all 9 Subjects
Abstract:
... We studied how storing fresh sardines (Sardina pilchardus) on ice for 0–15 days would affect lipid quality and sensory acceptance after the sardines were later canned. Average moisture and diacylglycerol contents showed a decreasing trend during storage time for sardines stored for to 0–10 days and an increasing trend for samples stored for 13–15 days. Total lipid and triacylglycerol average value ...
diacylglycerols, etc ; Saccharomyces cerevisiae; antibodies; dephosphorylation; energy; interphase; mice; phosphorylation; research; triacylglycerol lipase; triacylglycerols; yeasts; Show all 12 Subjects
Abstract:
... Triacylglycerols (TGs) serve as reservoirs for diacylglycerols and fatty acids, which play important roles in synthesizing energy and membrane lipids that are required for cell cycle progression. In the yeast, Saccharomyces cerevisiae, Tgl4, the functional ortholog of murine adipose triacylglycerol lipase (ATGL), is activated by Cdk1/Cdc28-mediated phosphorylation and facilitates the G1/S transiti ...
diacylglycerols, etc ; X-radiation; ambient temperature; crystallization; emulsions; ingredients; light microscopy; liquids; monoacylglycerols; oils; petroleum; rheology; Show all 12 Subjects
Abstract:
... The crystallization behavior of commercial mono- and diglycerides (MDG) in paraffin oil is studied to develop an in-depth understanding of the polymorphic transitions useful for the physical stability of petroleum oil-based topical emulsions. Optical microscopy and differential scanning calorimetry measurements showed the formation of plate-like and spherulite crystals at high and low temperatures ...
diacylglycerols, etc ; cocoa butter; food industry; food science; health promotion; microalgae; microbial growth; oleogels; phytochemicals; triacylglycerols; Show all 10 Subjects
Abstract:
... Omega-3 fatty acids and phytochemicals are known to offer essential functions needed to maintain the wellness of our body systems. Driven by the upsurging demand and the diminishing traditional sources, sustainable alternatives from agri-food sector by-products, native or genetically engineered microalgae and terrestrial plants are proposed. Herein, enzymatically synthesized structured lipids (e.g ...
... The Coccomyxa sp. strain Obi is a unicellular green alga that accumulates >40% triacylglycerol (TAG) per cell dry weight under nitrogen (N)-limiting conditions. In this study, we analyzed the de novo synthesis of TAG under N-replete and N-limiting conditions using ¹⁴C tracers and quantitative reverse transcription PCR (RT-qPCR) approaches. In cells grown under N-replete conditions, the incorporati ...
... Peach fruit is prone to chilling injury (CI) during long-term low temperature (LT) storage, and develops protective mechanisms against LT stress. This study revealed that 4 °C storage induced the occurrence of CI in peach fruit by promoting the expression of membrane lipid metabolism genes and phosphatidic acid (PA) accumulation, and stimulated a protective mechanism for peach fruit against LT by ...
... Punicic acid (PA)-enriched diacylglycerol (DAG) possesses both the beneficial effects of PA and DAG. However, few studies have focused on the preparation of PA-enriched DAG. Here, we report a novel and highly efficient method for the preparation of PA-enriched DAG through Lipozyme 435-catalyzed ethanolysis of pomegranate seed oil (PSO). Firstly, PSO was obtained through microwave-assisted extracti ...