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1. Top down proteomic analysis of gingival crevicular fluid in deciduous, exfoliating and permanent teeth in children

Author:
Iavarone, Federica; Olianas, Alessandra; Patini, Romeo; Gallenzi, Patrizia; Di Tonno, Laura; Desiderio, Claudia; Cabras, Tiziana; Manconi, Barbara; Vincenzoni, Federica; Cordaro, Massimo; Messana, Irene; Urbani, Andrea; Castagnola, Massimo
Source:
Journal of proteomics 2020 v.226 pp. 103890
ISSN:
1874-3919
Subject:
disease diagnosis,   etc   ; children; peptides; proteins; proteomics; teeth;     Show all 6 Subjects
Abstract:
... Gingival Crevicular Fluid (GCF), a plasma-derived exudate present in the gingival crevice was collected from deciduous, exfoliating and permanent teeth from 20 children (60 samples) with the aim to characterize and quantify by a mass spectrometry based top-down proteomic approach, the peptide/proteins in the fluid and verify possible variations occurring during the exfoliating process.The results ...
DOI:
10.1016/j.jprot.2020.103890

2. Reproducibility and validity of portable haemoglobinometer for the diagnosis of anaemia in children under the age of 5 years

Author:
da Silva Pereira, Alessandra; de Castro, Inês Rugani Ribeiro; Bezerra, Flávia Fioruci; Nogueira Neto, José Firmino; da Silva, Ana Carolina Feldenheimer
ISSN:
2048-6790
Subject:
disease diagnosis,   etc   ; anemia; automation; blood; blood sampling; children;     Show all 6 Subjects
Abstract:
... Portable haemoglobinometers have been used in order to estimate the prevalence of anaemia in diverse settings. However, few studies have been conducted to evaluate their performance in children of different age groups in distinct epidemiological contexts. To evaluate the reproducibility and reliability of a portable haemoglobinometer for the diagnosis of anaemia in children <5 years Hb was measure ...
DOI:
10.1017/jns.2019.43
PubMed:
32042411
PubMed Central:
PMC6984124

3. Correction to: Potential role of lncRNA-TSIX, miR-548-a-3p, and SOGA1 mRNA in the diagnosis of hepatocellular carcinoma

Author:
Habieb, Alaa; Matboli, Marwa; El-Tayeb, Hanaa; El-Asmar, Farid
Source:
Molecular biology reports 2019 v.46 no.4 pp. 4591
ISSN:
0301-4851
Subject:
disease diagnosis,   etc   ; hepatoma; messenger RNA;     Show all 3 Subjects
Abstract:
... The original publication has been updated. The family name of Alaa Habieb contained a typo that has now been corrected. ...
DOI:
10.1007/s11033-019-04838-z

4. Significance and interpretation of molecular diagnostics for insecticide resistance management of agricultural pests

Author:
Van Leeuwen, Thomas; Dermauw, Wannes; Mavridis, Konstantinos; Vontas, John
Source:
Current opinion in insect science 2020 v.39 pp. 69-76
ISSN:
2214-5745
Subject:
disease diagnosis,   etc   ; decision making; diagnostic techniques; genetic markers; insecticide resistance; insecticides; monitoring; pests; resistance management;     Show all 9 Subjects
Abstract:
... Insecticide resistant pests become increasingly difficult to control in current day agriculture. Because of environmental and health concerns, the insecticide portfolio to combat agricultural pests is gradually decreasing. It is therefore crucial to make rational decisions on insecticide use to assure effective resistance management. However, resistance monitoring programs that inform on pest susc ...
DOI:
10.1016/j.cois.2020.03.006

5. Donor effect dominated molybdenum disulfide/graphene nanostructure-based field-effect transistor for ultrasensitive DNA detection

Author:
Chen, Shuo; Sun, Yang; Xia, Yaping; Lv, Ke; Man, Baoyuan; Yang, Cheng
Source:
Biosensors & bioelectronics 2020 v.156 pp. 112128
ISSN:
0956-5663
Subject:
disease diagnosis,   etc   ; biosensors; detection limit; graphene; molybdenum disulfide; nanomaterials; nucleic acid hybridization; transistors;     Show all 8 Subjects
Abstract:
... Field effect transistor (FET) biosensors based on low-dimensional materials have the advantages of small in size, simple structure, fast response and high sensitivity. In this work, a field-effect transistor biosensor based on molybdenum disulfide/graphene (MoS₂/graphene) hybrid nanostructure was proposed and fabricated for DNA hybridization detection. The biosensor achieved an effective response ...
DOI:
10.1016/j.bios.2020.112128

6. Assessing the range of enzymatic and oxidative tunability for biosensor design

Author:
Schunk, Hattie C.; Hernandez, Derek S.; Austin, Mariah J.; Dhada, Kabir S.; Rosales, Adrianne M.; Suggs, Laura J.
Source:
Journal of materials chemistry B 2020 v.8 no.16 pp. 3460-3487
ISSN:
2050-7518
Subject:
disease diagnosis,   etc   ; biocompatible materials; biosensors; enzyme activity; inflammation; monitoring; nanomaterials; oxidative stress; research and development; therapeutics;     Show all 10 Subjects
Abstract:
... Development of multi-functional materials and biosensors that can achieve an in situ response designed by the user is a current need in the biomaterials field, especially in complex biological environments, such as inflammation, where multiple enzymatic and oxidative signals are present. In the past decade, there has been extensive research and development of materials chemistries for detecting an ...
DOI:
10.1039/c9tb02666e
PubMed:
32159202
PubMed Central:
PMC7219111

7. Owner Compliance to an Environmental Management Protocol for Severe Equine Asthma Syndrome

Author:
Simões, Joana; Sales Luís, José P.; Tilley, Paula
Source:
Journal of equine veterinary science 2020 v.87 pp. 102937
ISSN:
0737-0806
Subject:
disease diagnosis,   etc   ; adults; asthma; compliance; disease progression; dust; environmental management; horses; protocols;     Show all 9 Subjects
Abstract:
... Severe equine asthma (SEA) syndrome is a chronic recurrent respiratory disease, common among adult horses. The disease occurs in genetically susceptible individuals after their exposure to organic dust. Thus, environmental management has proved essential in controlling airway challenge and disease exacerbation. This is a demanding process that can only be achieved through the horse owners’ coopera ...
DOI:
10.1016/j.jevs.2020.102937

8. Aptamer-Cholesterol-Mediated Proximity Ligation Assay for Accurate Identification of Exosomes

Author:
Zhao, Xianxian; Luo, Canjun; Mei, Qiang; Zhang, Hongmin; Zhang, Wenqing; Su, Dongwei; Fu, Weiling; Luo, Yang
Source:
Analytical chemistry 2020 v.92 no.7 pp. 5411-5418
ISSN:
1520-6882
Subject:
disease diagnosis,   etc   ; biomarkers; cholesterol; engineering; exosomes; magnetism; oligonucleotides; precision medicine; surface proteins;     Show all 9 Subjects
Abstract:
... Accurate identification of exosomes plays an essential role in facilitating disease diagnosis and therapies. Herein, we proposed an Aptamer-cholesterol-mediated Proximity Ligation Assay (AcmPLA) for accurate identification of exosomes in a dual-probe strategy, one aptamer probe for recognition of exosomal innate surface protein CD63 and another cholesterol probe for biolipid layer targeting. By in ...
DOI:
10.1021/acs.analchem.0c00141

9. Skin-like Ultrasensitive Strain Sensor for Full-Range Detection of Human Health Monitoring

Author:
Sun, Zijing; Yang, Shuo; Zhao, Pengfei; Zhang, Junmo; Yang, Yahan; Ye, Xiaolin; Zhao, Xiaoli; Cui, Nan; Tong, Yanhong; Liu, Yichun; Chen, Xi; Tang, Qingxin
Source:
ACS applied materials & interfaces 2020 v.12 no.11 pp. 13287-13295
ISSN:
1944-8252
Subject:
disease diagnosis,   etc   ; carbon nanotubes; detection limit; durability; gold; human health; monitoring;     Show all 7 Subjects
Abstract:
... The development of strain sensors with high sensitivity and stretchability, which can accurately detect different human activities such as subtle physiological signals and large-scale joint motions is essential for disease diagnosis and human health monitoring. However, achieving both high sensitivity and stretchability is still an enormous challenge at the moment, particularly for intrinsically s ...
DOI:
10.1021/acsami.9b21751

10. A disease inference method based on symptom extraction and bidirectional Long Short Term Memory networks

Author:
Guo, Donglin; Duan, Guihua; Yu, Ying; Li, Yaohang; Wu, Fang-Xiang; Li, Min
Source:
Methods 2020 v.173 pp. 75-82
ISSN:
1046-2023
Subject:
disease diagnosis,   etc   ; data collection; databases; medical records; medical treatment; neural networks; patients; uncertainty;     Show all 8 Subjects
Abstract:
... The wide applications of automatic disease inference in many medical fields improve the efficiency of medical treatments. Many efforts have been made to predict patients’ future health conditions according to their full clinical texts, clinical measurements or medical codes. Symptoms reflect the onset of diseases and can provide credible information for disease diagnosis. In this study, we propose ...
DOI:
10.1016/j.ymeth.2019.07.009

11. Implementation of a classification strategy of Raman data collected in different clinical conditions: application to the diagnosis of chronic lymphocytic leukemia

Author:
Féré, M.; Gobinet, C.; Liu, L. H.; Beljebbar, A.; Untereiner, V.; Gheldof, D.; Chollat, M.; Klossa, J.; Chatelain, B.; Piot, O.
Source:
Analytical and bioanalytical chemistry 2020 v.412 no.4 pp. 949-962
ISSN:
1618-2642
Subject:
disease diagnosis,   etc   ; Raman spectroscopy; blood; cell lines; data collection; glass; lymphocytic leukemia; models; patients;     Show all 9 Subjects
Abstract:
... The literature is rich in proof of concept studies demonstrating the potential of Raman spectroscopy for disease diagnosis. However, few studies are conducted in a clinical context to demonstrate its applicability in current clinical practice and workflow. Indeed, this translational research remains far from the patient’s bedside for several reasons. First, samples are often cultured cell lines. S ...
DOI:
10.1007/s00216-019-02321-z

12. Prevalence and diagnostic value of the ultrasonographic honeycomb appearance of the spleen in cats

Author:
Harel, Mathieu; Touzet, Chloe; Barthélemy, Anthony; Ségard-Weisse, Emilie M
Source:
Journal of feline medicine and surgery 2020 v.22 no.2 pp. 186-192
ISSN:
1532-2750
Subject:
disease diagnosis,   etc   ; blood; cats; cell biology; hematopoiesis; hemoglobin; hyperplasia; medical records; neoplasms; spleen; ultrasonics; ultrasonography;     Show all 12 Subjects
Abstract:
... The aim of this study was to report the prevalence of a honeycomb appearance of the spleen in a population of referral cats presented for ultrasound examination, and to determine the diagnostic value of this finding vs the definitive diagnosis, the splenic cytological and haematological results. Data were obtained from the medical records (2016–2018) of cats that had an ultrasonographic honeycomb ...
DOI:
10.1177/1098612X19837336

13. Robust index-based semantic plant/background segmentation for RGB- images

Author:
Riehle, Daniel; Reiser, David; Griepentrog, Hans W.
Source:
Computers and electronics in agriculture 2020 v.169 pp. 105201
ISSN:
0168-1699
Subject:
disease diagnosis,   etc   ; algorithms; automation; cameras; color; digital images; image analysis; models; yield forecasting;     Show all 9 Subjects
Abstract:
... Plant/background segmentation is a key component of digital image analysis in agriculture. It can be used for yield prediction models, crop growth, disease diagnosis and automated navigation tasks. In particular, well-known segmentation methods are strongly influenced by changing light conditions during image acquisition as well as different color variations of plants and the background in the sce ...
DOI:
10.1016/j.compag.2019.105201

14. CRISPR/Cas Multiplexed Biosensing: A Challenge or an Insurmountable Obstacle?

Author:
Li, Yi; Liu, Linyang; Liu, Guozhen
Source:
Trends in biotechnology 2019 v.37 no.8 pp. 792-795
ISSN:
0167-7799
Subject:
disease diagnosis,   etc   ; diagnostic techniques; gene editing;     Show all 3 Subjects
Abstract:
... Performing multiplex detection is still an elusive goal for molecular diagnostics. CRISPR/Cas-based biosensing has demonstrated potential for multiplex detection. Instead of being an insurmountable obstacle, CRISPR/Cas multiplexed biosensing is a realistic challenge with some recent successful applications. Strategic considerations are required to fully explore its potential in multiplex diagnosti ...
DOI:
10.1016/j.tibtech.2019.04.012

15. Diagnosis of spontaneous hyperadrenocorticism in dogs. Part 2: Adrenal function testing and differentiating tests

Author:
Bennaim, Michael; Shiel, Robert E.; Mooney, Carmel T.
Source:
The veterinary journal 2019 pp. 105343
ISSN:
1090-0233
Subject:
disease diagnosis,   etc   ; dog diseases; dogs; hyperadrenocorticism;     Show all 4 Subjects
Abstract:
... Hyperadrenocorticism is a relatively common endocrine disorder in dogs that has been extensively described. However, its diagnosis remains challenging because there is no true reference standard test, and a myriad of factors can affect the diagnostic performance of the commonly used adrenal function tests. Ultimately, the diagnosis is based on a combination of signalment, history and clinical find ...
DOI:
10.1016/j.tvjl.2019.105343

16. A Diagnosis for All Rare Genetic Diseases: The Horizon and the Next Frontiers

Author:
Boycott, Kym M.; Hartley, Taila; Biesecker, Leslie G.; Gibbs, Richard A.; Innes, A. Micheil; Riess, Olaf; Belmont, John; Dunwoodie, Sally L.; Jojic, Nebojsa; Lassmann, Timo; Mackay, Deborah; Temple, I. Karen; Visel, Axel; Baynam, Gareth
Source:
Cell 2019 v.177 no.1 pp. 32-37
ISSN:
0092-8674
Subject:
disease diagnosis,   etc   ; genetic disorders; patients; sequence analysis;     Show all 4 Subjects
Abstract:
... The introduction of exome sequencing in the clinic has sparked tremendous optimism for the future of rare disease diagnosis, and there is exciting opportunity to further leverage these advances. To provide diagnostic clarity to all of these patients, however, there is a critical need for the field to develop and implement strategies to understand the mechanisms underlying all rare diseases and tra ...
DOI:
10.1016/j.cell.2019.02.040

17. Hybridization chain reaction-based nanoprobe for cancer cell recognition and amplified photodynamic therapy

Author:
Xiong, Mengyi; Rong, Qiming; Kong, Gezhi; Yang, Chan; Zhao, Yan; Qu, Feng-Li; Zhang, Xiao-Bing; Tan, Weihong
Source:
Chemical communications 2019 v.55 no.21 pp. 3065-3068
ISSN:
1364-548X
Subject:
disease diagnosis,   etc   ; nanotechnology; neoplasm cells; neoplasms; photochemotherapy;     Show all 5 Subjects
Abstract:
... Precision diagnosis and effective treatment are the cores of early cancer therapy. Here, for the first time, we report a hybridization chain reaction-based nanoprobe for selective and sensitive cancer cell recognition and amplified photodynamic therapy. ...
DOI:
10.1039/c8cc10074h

18. Diagnostic MS/MS fragmentation patterns for the discrimination between Schiff bases and their Amadori or Heyns rearrangement products

Author:
Xing, Haoran; Mossine, Valeri V.; Yaylayan, Varoujan
Source:
Carbohydrate research 2020 v.491 pp. 107985
ISSN:
0008-6215
Subject:
disease diagnosis,   etc   ; Maillard reaction; energy; furans; glucose; glycine (amino acid); ions; moieties; schiff bases; sodium; tandem mass spectrometry;     Show all 11 Subjects
Abstract:
... Schiff bases, the Amadori and Heyns rearrangement products are the most important isomeric intermediates involved in the early Maillard reaction; distinguishing between them by analytical mass spectroscopic techniques remains a challenge. Here we demonstrate that MS/MS fragmentation patterns can be used for the discrimination between glucose derived Schiff bases, Amadori, and Heyns compounds with ...
DOI:
10.1016/j.carres.2020.107985

19. Phosphoprotein Biosensors for Monitoring Pathological Protein Structural Changes

Author:
Ahmed, Mostak; Koo, Kevin M.; Mainwaring, Paul N.; Carrascosa, Laura G.; Trau, Matt
Source:
Trends in biotechnology 2020 v.38 no.5 pp. 519-531
ISSN:
0167-7799
Subject:
disease diagnosis,   etc   ; biosensors; cost effectiveness; genomics; monitoring; neoplasms; phosphoproteins; phosphorylation; post-translational modification; protein structure; proteomics; therapeutics; transcriptomics;     Show all 13 Subjects
Abstract:
... Current biotechnological developments are driving a significant shift towards integrating proteomic analysis with landmark genomic, methylomic, and transcriptomic data to elucidate functional effects. For the majority of proteins, structure and function are closely intertwined. Post-translational protein modifications (e.g., phosphorylation) leading to aberrantly active structures can originate a ...
DOI:
10.1016/j.tibtech.2019.11.006

20. Colorimetric Polymerase Chain Reaction Enabled by a Fast Light-Activated Substrate Chromogenic Detection Platform

Author:
Dong, Tianyu; Mansour, Hayam; Hu, Hao; Wang, Guan A.; Watson, Colton J. F.; Yousef, Michael; Matamoros, Gabriela; Sanchez, Ana L.; MacNeil, Adam J.; Wu, Peng; Li, Feng
Source:
Analytical chemistry 2020 v.92 no.9 pp. 6456-6461
ISSN:
1520-6882
Subject:
disease diagnosis,   etc   ; DNA; color; colorimetry; detection limit; dyes; fluorescence; photooxidation; point-of-care systems; quantitative polymerase chain reaction; singlet oxygen;     Show all 11 Subjects
Abstract:
... Miniaturization of nucleic acid tests (NATs) into portable, inexpensive detection platforms may aid disease diagnosis in point-of-care (POC) settings. Colorimetric signals are ideal readouts for portable NATs, and it remains of high demand to develop color readouts that are simple, quantitative, and versatile. Thus motivated, we report a fast light-activated substrate chromogenic polymerase chain ...
DOI:
10.1021/acs.analchem.9b05591