U.S. flag

An official website of the United States government

PubAg

Main content area

You searched for: Subject "double-stranded RNA" Remove constraint Subject: "double-stranded RNA"
Start Over

Search

2,781  Search Results

« Previous | 1 - 20 of 2,781 |
Number of results to display per page

Search Results

1. Structure Unveils Relationships between RNA Virus Polymerases

Author:
Mönttinen, Heli A. M.; Ravantti, Janne J.; Poranen, Minna M.
Source:
Viruses 2021 v.13 no.2
ISSN:
1999-4915
Subject:
double-stranded RNA,   etc   ; RNA primers; automation; phylogeny; sequence homology; topology;     Show all 6 Subjects
Abstract:
... RNA viruses are the fastest evolving known biological entities. Consequently, the sequence similarity between homologous viral proteins disappears quickly, limiting the usability of traditional sequence-based phylogenetic methods in the reconstruction of relationships and evolutionary history among RNA viruses. Protein structures, however, typically evolve more slowly than sequences, and structura ...
DOI:
10.3390/v13020313

2. A unified genotypic classification of infectious bursal disease virus based on both genome segments

Author:
Islam, Mohammad Rafiqul; Nooruzzaman, Mohammed; Rahman, Tazinur; Mumu, Tanjin Tamanna; Rahman, Mohammad Mijanur; Chowdhury, Emdadul Haque; Eterradossi, Nicolas; Müller, Hermann
Source:
Avian pathology 2021 v.50 no.2 pp. 190-206
ISSN:
1465-3338
Subject:
double-stranded RNA,   etc   ; Infectious bursal disease virus; genome; phylogeny; serotypes; virulence;     Show all 6 Subjects
Abstract:
... Infectious bursal disease virus (IBDV) of chickens is a birnavirus with a bi-segmented double-stranded RNA genome, the segments designated as A and B. We performed phylogenetic analysis using a 366-bp fragment of segment A (nt 785–1150) and a 508-bp fragment of segment B (nt 328–835) of IBDV. A total of 463 segment A and 434 segment B sequences from GenBank, including the sequences of eight recent ...
DOI:
10.1080/03079457.2021.1873245

3. ABCE1 Regulates RNase L-Induced Autophagy during Viral Infections

Author:
Ramnani, Barkha; Manivannan, Praveen; Jaggernauth, Sarah; Malathi, Krishnamurthy
Source:
Viruses 2021 v.13 no.2
ISSN:
1999-4915
Subject:
double-stranded RNA,   etc   ; ; antiviral properties; apoptosis; autophagy; caspase-3; cell proliferation; interferons; ribonucleases; virus replication;     Show all 10 Subjects
Abstract:
... Host response to a viral infection includes the production of type I interferon (IFN) and the induction of interferon-stimulated genes that have broad antiviral effects. One of the key antiviral effectors is the IFN-inducible oligoadenylate synthetase/ribonuclease L (OAS/RNase L) pathway, which is activated by double-stranded RNA to synthesize unique oligoadenylates, 2-5A, to activate RNase L. RNa ...
DOI:
10.3390/v13020315

4. Western Corn Rootworm, Plant and Microbe Interactions: A Review and Prospects for New Management Tools

Author:
Paddock, Kyle J.; Robert, Christelle A. M.; Erb, Matthias; Hibbard, Bruce E.
Source:
Insects 2021 v.12 no.2
ISSN:
2075-4450
Subject:
double-stranded RNA,   etc   ; Diabrotica virgifera virgifera; RNA interference; biological control; crop rotation; herbivores;     Show all 6 Subjects
Abstract:
... The western corn rootworm, Diabrotica virgifera virgifera LeConte, is resistant to four separate classes of traditional insecticides, all Bacillius thuringiensis (Bt) toxins currently registered for commercial use, crop rotation, innate plant resistance factors, and even double-stranded RNA (dsRNA) targeting essential genes via environmental RNA interference (RNAi), which has not been sold commerc ...
DOI:
10.3390/insects12020171

5. Nanoparticle‐mediated double‐stranded RNA delivery system: A promising approach for sustainable pest management

Author:
Yan, Shuo; Ren, Bin‐Yuan; Shen, Jie
Source:
Insect science 2021 v.28 no.1 pp. 21-34
ISSN:
1672-9609
Subject:
double-stranded RNA,   etc   ; RNA interference; chitosan; dendrimers; insects; lethal genes; nanoparticles; pesticides; plant protection;     Show all 9 Subjects
Abstract:
... RNA interference (RNAi) targeting lethal genes in insects has great potential for sustainable crop protection. Compared with traditional double‐stranded (ds)RNA delivery systems, nanoparticles such as chitosan, liposomes, and cationic dendrimers offer advantages in delivering dsRNA/small interfering (si)RNA to improve RNAi efficiency, thus promoting the development and practice of RNAi‐based pest ...
DOI:
10.1111/1744-7917.12822

6. Spliceosome-targeted therapies trigger an antiviral immune response in triple-negative breast cancer

Author:
Bowling, Elizabeth A.; Wang, Jarey H.; Gong, Fade; Wu, William; Neill, Nicholas J.; Kim, Ik Sun; Tyagi, Siddhartha; Orellana, Mayra; Kurley, Sarah J.; Dominguez-Vidaña, Rocio; Chung, Hsiang-Ching; Hsu, Tiffany Y.-T.; Dubrulle, Julien; Saltzman, Alexander B.; Li, Heyuan; Meena, Jitendra K.; Canlas, Gino M.; Chamakuri, Srinivas; Singh, Swarnima; Simon, Lukas M.; Olson, Calla M.; Dobrolecki, Lacey E.; Lewis, Michael T.; Zhang, Bing; Golding, Ido; Rosen, Jeffrey M.; Young, Damian W.; Malovannaya, Anna; Stossi, Fabio; Miles, George; Ellis, Matthew J.; Yu, Lihua; Buonamici, Silvia; Lin, Charles Y.; Karlin, Kristen L.; Zhang, Xiang H.-F.; Westbrook, Thomas F.
Source:
Cell 2021 v.184 no.2 pp. 384-403.e21
ISSN:
0092-8674
Subject:
double-stranded RNA,   etc   ; apoptosis; breast neoplasms; breasts; cold; humans; hypersensitivity; immune response; neoplasm cells;     Show all 9 Subjects
Abstract:
... Many oncogenic insults deregulate RNA splicing, often leading to hypersensitivity of tumors to spliceosome-targeted therapies (STTs). However, the mechanisms by which STTs selectively kill cancers remain largely unknown. Herein, we discover that mis-spliced RNA itself is a molecular trigger for tumor killing through viral mimicry. In MYC-driven triple-negative breast cancer, STTs cause widespread ...
DOI:
10.1016/j.cell.2020.12.031

7. Characterization, expression patterns, and transcriptional responses of three core RNA interference pathway genes from Ostrinia nubilalis

Author:
Cooper, Anastasia M.W.; Song, Huifang; Shi, Xuekai; Yu, Zhitao; Lorenzen, Marcé; Silver, Kristopher; Zhang, Jianzhen; Zhu, Kun Yan
Source:
Journal of insect physiology 2021 v.129 pp. 104181
ISSN:
0022-1910
Subject:
double-stranded RNA,   etc   ; ; Ostrinia nubilalis; RNA interference; amino acid sequences; enzyme activity; genes; ingestion; insect physiology; ribonucleases; transcription (genetics);     Show all 11 Subjects
Abstract:
... RNA interference (RNAi) is commonly used in the laboratory to analyze gene function, and RNAi-based pest management strategies are now being employed. Unfortunately, RNAi is hindered by inefficient and highly-variable results when different insects are targeted, especially lepidopterans, such as the European corn borer (ECB), Ostrinia nubilalis (Lepidoptera: Crambidae). Previous efforts to achieve ...
DOI:
10.1016/j.jinsphys.2020.104181

8. Extensive Phylogenetic Analysis of Piscine Orthoreovirus Genomic Sequences Shows the Robustness of Subgenotype Classification

Author:
Godoy, Marcos; Medina, Daniel A.; Suarez, Rudy; Valenzuela, Sandro; Romero, Jaime; Kibenge, Molly; Wang, Yingwei; Kibenge, Frederick
Source:
Pathogens 2021 v.10 no.1
ISSN:
2076-0817
Subject:
double-stranded RNA,   etc   ; ; Orthoreovirus; Salmonidae; fish; genetic databases; genetic variation; genome; genomics; host specificity; phylogeny; sequence analysis; subgenotype; virulence;     Show all 14 Subjects
Abstract:
... Piscine orthoreovirus (PRV) belongs to the family Reoviridae and has been described mainly in association with salmonid infections. The genome of PRV consists of about 23,600 bp, with 10 segments of double-stranded RNA, classified as small (S1 to S4), medium (M1, M2 and M3) and large (L1, L2 and L3); these range approximately from 1000 bp (segment S4) to 4000 bp (segment L1). How the genetic varia ...
DOI:
10.3390/pathogens10010041

9. Plant Functional Genomics in A Few Days: Laser-Assisted Delivery of Double-Stranded RNA to Higher Plants

Author:
Killiny, Nabil; Gonzalez-Blanco, Pedro; Gowda, Siddarame; Martini, Xavier; Etxeberria, Ed
Source:
Plants 2021 v.10 no.1
ISSN:
2223-7747
Subject:
double-stranded RNA,   etc   ; Citrus; bleaching; cells; genomics; leaves; light; phenotype; phloem; reporter genes; technology; transgenic plants; trees;     Show all 13 Subjects
Abstract:
... The technology of transgenic plants is challenging and time consuming, especially for higher plants and trees such as citrus. Double-stranded RNA (dsRNA) delivery via a plant virus is an alternative method to create transgenic plants by suppressing the expression of plant endogenous genes. Citrus tristeza virus-based vector has been constructed specifically for use in citrus trees. However, this i ...
DOI:
10.3390/plants10010093

10. RS_CRZ1, a C2H2-Type Transcription Factor Is Required for Pathogenesis of Rhizoctonia solani AG1-IA in Tomato

Author:
Ghosh, Srayan; Kant, Ravi; Pradhan, Amrita; Jha, Gopaljee
Source:
Molecular plant-microbe interactions 2021 v.34 no.1 pp. 26-38
ISSN:
1943-7706
Subject:
double-stranded RNA,   etc   ; Thanatephorus cucumeris; genes; genomics; mutants; pathogenesis; pathogenicity; pathogens; proteinases; tomatoes; transcription factors; transcriptomics; yeasts; zinc finger motif;     Show all 14 Subjects
Abstract:
... Rhizoctonia solani is a necrotrophic fungal pathogen that causes disease in diverse plant species. In recent years, the genomic and transcriptomic studies have identified several candidate pathogenicity determinants of R. solani; however, most of them remain to be validated. In this study, we report a viral vector-based host-induced gene silencing (HIGS) as well as a dsRNA (double-stranded RNA)-ba ...
DOI:
10.1094/MPMI-05-20-0121-R

11. A Chemosensory Protein Detects Antifeedant in Locust (Locusta migratoria)

Author:
Jiang, Xingcong; Xu, Haozhi; Zheng, Nan; Yin, Xuewei; Zhang, Long
Source:
Insects 2020 v.12 no.1
ISSN:
2075-4450
Subject:
double-stranded RNA,   etc   ; Locusta migratoria; antifeedants; bioassays; fluorescence; food consumption;     Show all 6 Subjects
Abstract:
... Chemosensory system is vitally important for animals to select food. Antifeedants that herbivores encounter can interfere with feeding behavior and exert physiological effects. Few studies have assessed the molecular mechanisms underlying the chemoreception of antifeedants. In this study, we demonstrated that a chemosensory protein (CSP) in Locusta migratoria is involved in detecting an antifeedan ...
DOI:
10.3390/insects12010001

12. Double-stranded RNA confers resistance to pepper mottle virus in Nicotiana benthamiana

Author:
Yoon, Jeongyeon; Fang, Miao; Lee, Dowhan; Park, Minsu; Kim, Kook-Hyung; Shin, Chanseok
Source:
Applied biological chemistry 2021 v.64 no.1 pp. 1
ISSN:
2468-0834
Subject:
double-stranded RNA,   etc   ; Nicotiana benthamiana; Pepper mottle virus; RNA interference; chemistry; gene expression; genes; host plants; infection; injection; leaves; plant protection; vaccines; viral growth; viruses;     Show all 15 Subjects
Abstract:
... RNA interference (RNAi) is a regulatory mechanism of gene expression mediated by small RNAs. By using the RNAi technique, exogenous double-stranded RNA (dsRNA) designed to target mRNA, suppresses target gene expression levels in plants. In this study, we adopted the RNAi mechanism as a tool to protect plants from viruses. We designed and synthesized several dsRNAs targeting the pepper mottle virus ...
DOI:
10.1186/s13765-020-00581-3

13. PU14, a Novel Matrix Protein, Participates in Pearl Oyster, Pinctada Fucata, Shell Formation

Author:
Ji, Yinghui; Yang, Xue; Yang, Dong; Zhang, Rongqing
Source:
Marine biotechnology 2021 v.23 no.2 pp. 189-200
ISSN:
1436-2228
Subject:
double-stranded RNA,   etc   ; Pinctada fucata; amino acid sequences; biomineralization; biotechnology; calcite; gene expression; isoelectric point; molecular weight; nucleotide sequences; recombinant proteins;     Show all 11 Subjects
Abstract:
... Biomineralization is a widespread biological process, involved in the formation of shells, teeth, and bones. Shell matrix proteins have been widely studied for their importance during shell formation. In 2015, our group identified 72 unique shell matrix proteins in Pinctada fucata, among which PU14 is a matrix protein detected in the soluble fraction that solely exists in the prismatic layer. Howe ...
DOI:
10.1007/s10126-020-10014-3

14. RNA interference targeting ecdysone receptor blocks the larval–pupal transition in Henosepilachna vigintioctopunctata

Author:
Wu, Jian‐Jian; Mu, Li‐Li; Kang, Wei‐Nan; Ze, Long‐Ji; Shen, Chen‐Hui; Jin, Lin; Anjum, Ahmad Ali; Li, Guo‐Qing
Source:
Insect science 2021 v.28 no.2 pp. 419-429
ISSN:
1672-9609
Subject:
double-stranded RNA,   etc   ; ; Cucurbitaceae; Escherichia coli; Henosepilachna vigintioctopunctata; RNA interference; bioassays; ecdysone receptor; genes; greenhouses; human health; ingestion; insect pests; leaves; pesticides; pollution; potatoes; prepupae; Australia; Brazil;     Show all 20 Subjects
Abstract:
... Henosepilachna vigintioctopunctata is a serious insect pest which attacks a large number of nightshades and cucurbits in Asian countries, Brazil and Australia. Prolonged application of traditional pesticides has caused environmental pollution and exerted deleterious effects on human health. Finding new approaches with high target specificity and low environmental contamination has become an urgent ...
DOI:
10.1111/1744-7917.12777

15. Presymptomatic viral shedding and infective ability of SARS-CoV-2; a case report

Author:
Nissen, Karolina; Hagbom, Marie; Krambrich, Janina; Akaberi, Dario; Sharma, Sumit; Ling, Jiaxin; Hoffman, Tove; Svensson, Lennart; Bondeson, Kåre; Salaneck, Erik
Source:
Heliyon 2021 v.7 no.2 pp. e06328
ISSN:
2405-8440
Subject:
double-stranded RNA,   etc   ; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; case studies; cytopathogenicity; fluorescent antibody technique; health care workers;     Show all 7 Subjects
Abstract:
... Possible pre- or asymptomatic transmission has been reported, both from SARS-CoV and from MERS-CoV outbreaks, although this appears to be uncommon. In contrast, during the COVID-19 pandemic, an increasing number of studies and case reports indicate that pre- or asymptomatic transmission of SARS-CoV-2 is not only possible but also occurs frequently. We report repeated rRT-PCR detection of SARS-CoV- ...
DOI:
10.1016/j.heliyon.2021.e06328

16. The Capsid Protein of Rubella Virus Antagonizes RNA Interference in Mammalian Cells

Author:
Xu, Jiuyue; Kong, Jing; Lyu, Bao; Wang, Xiaotong; Qian, Qi; Zhou, Xi; Qiu, Yang
Source:
Viruses 2021 v.13 no.2
ISSN:
1999-4915
Subject:
double-stranded RNA,   etc   ; RNA interference; Rubella virus; capsid; coat proteins; congenital abnormalities; dimerization; eukaryotic cells; mammals; pathogens; public health; replicon; therapeutics; viruses;     Show all 14 Subjects
Abstract:
... Rubella virus (RuV) is the infectious agent of a series of birth defect diseases termed congenital rubella syndrome, which is a major public health concern all around the world. RNA interference (RNAi) is a crucial antiviral defense mechanism in eukaryotes, and numerous viruses have been found to encode viral suppressors of RNAi (VSRs) to evade antiviral RNAi response. However, there is little kno ...
DOI:
10.3390/v13020154

17. MicroRNA-Mediated Responses to Cadmium Stress in Arabidopsis thaliana

Author:
Pegler, Joseph L.; Oultram, Jackson M. J.; Nguyen, Duc Quan; Grof, Christopher P. L.; Eamens, Andrew L.
Source:
Plants 2021 v.10 no.1
ISSN:
2223-7747
Subject:
double-stranded RNA,   etc   ; Arabidopsis thaliana; RNA interference; anthropogenic activities; cadmium; environment; gene expression; heavy metals; microRNA; mutants; phenotype; roots; shoots; soil; toxicity;     Show all 15 Subjects
Abstract:
... In recent decades, the presence of cadmium (Cd) in the environment has increased significantly due to anthropogenic activities. Cd is taken up from the soil by plant roots for its subsequent translocation to shoots. However, Cd is a non-essential heavy metal and is therefore toxic to plants when it over-accumulates. MicroRNA (miRNA)-directed gene expression regulation is central to the response of ...
DOI:
10.3390/plants10010130

18. An improved 4′-aminomethyltrioxsalen-based nucleic acid crosslinker for biotinylation of double-stranded DNA or RNA

Author:
Wielenberg, Kevin; Wang, Miao; Yang, Min; Ozer, Abdullah; Lis, John T.; Lin, Hening
Source:
RSC advances 2020 v.10 no.65 pp. 39870-39874
ISSN:
2046-2069
Subject:
double-stranded RNA,   etc   ; DNA; biotinylation; chemical bonding; crosslinking; drugs; moieties; psoralen; ultraviolet radiation;     Show all 9 Subjects
Abstract:
... Nucleic acid crosslinkers that covalently join complementary strands of DNA/RNA have applications in both pharmaceuticals and as biochemical probes. Psoralen is a popular crosslinking moiety that reacts with double stranded DNA and RNA upon exposure to longwave UV light. The commercially available compound EZ-link psoralen-PEG3-biotin has been used in numerous studies to crosslink DNA and double-s ...
DOI:
10.1039/d0ra07437c

19. Dual roles of juvenile hormone signaling during early oogenesis in Drosophila

Author:
Luo, Wei; Veeran, Sethuraman; Wang, Jian; Li, Sheng; Li, Kang; Liu, Su‐Ning
Source:
Insect science 2020 v.27 no.4 pp. 665-674
ISSN:
1672-9609
Subject:
double-stranded RNA,   etc   ; Drosophila; germ cells; insects; juvenile hormones; metamorphosis; mutants; myocytes; oogenesis; stem cells;     Show all 10 Subjects
Abstract:
... Juvenile hormone (JH) signaling plays crucial roles in insect metamorphosis and reproduction. Function of JH signaling in germline stem cells (GSCs) remains largely unknown. Here, we found that the number of GSCs significantly declined in the ovaries of Met, Gce and JHAMT mutants. Then we inhibited JH signaling in selected cell types of ovaries by expressing Met and Gce or Kr‐h1 double‐stranded RN ...
DOI:
10.1111/1744-7917.12698

20. A new cypovirus from the Japanese peppered moth, Biston robustus

Author:
Takatsuka, Jun
Source:
Journal of invertebrate pathology 2020 v.174 pp. 107417
ISSN:
0022-2011
Subject:
double-stranded RNA,   etc   ; Biston betularia; Bombyx mori; Cypovirus; conserved sequences; genome; proteins; silkworms;     Show all 8 Subjects
Abstract:
... A cypovirus was isolated from larvae of the Japanese peppered moth, Biston robustus. The viral genome is 23,954 bp comprising 10 segmented double-stranded RNAs with a new electropherotype among cypoviruses. Each segment encodes one putative protein and has non-coding regions that contain conserved sequences at their 5′ and 3′ termini, 5′-AGAA(U/A)U-3′ and 5′-UGC-3′, respectively. Seven proteins en ...
DOI:
10.1016/j.jip.2020.107417