U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject "double-stranded RNA" Remove constraint Subject: "double-stranded RNA"
Start Over

Search

3,065  Search Results

« Previous | 1 - 20 of 3,065 |
Number of results to display per page

Search Results

1. Enrichment and recovery of oligonucleotide impurities by N-Rich twin-column continuous chromatography

Author:
Giulio Lievore; Richard Weldon; Martina Catani; Alberto Cavazzini; Thomas Müller-Späth
Source:
Journal of chromatography 2022 v.1209 pp. 123439
ISSN:
1570-0232
Subject:
double-stranded RNA,   etc   ; chromatography; oligonucleotides; therapeutics;     Show all 4 Subjects
Abstract:
... N-Rich is a twin-column continuous chromatography technology well suited for small-scale isolation and the enrichment of product related impurities. For the first time, N-Rich was used for impurity isolation from a double-stranded RNA (dsRNA) therapeutic synthetic oligonucleotide (ON), produced by solid-phase synthesis. By employing the N-Rich process, where the desired impurities are recycled and ...
DOI:
10.1016/j.jchromb.2022.123439

2. TIR domains as two-tiered enzymes to activate plant immunity

Author:
Lei Tian; Xin Li
Source:
Cell 2022 v.185 no.13 pp. 2208-2209
ISSN:
0092-8674
Subject:
double-stranded RNA,   etc   ; DNA; cell death; enzymes; pathogens;     Show all 5 Subjects
Abstract:
... Plant immune receptors often contain TIR domains, which can oligomerize to form active enzyme complexes in response to pathogen infections. In this issue of Cell, Yu and colleagues discover that some plant TIR domains possess a novel 2′,3′-cAMP/cGMP synthetase activity that cleaves double-stranded RNA/DNA, triggering cell death during plant immune responses. ...
DOI:
10.1016/j.cell.2022.05.025

3. Mammalian viral suppressors of RNA interference

Author:
Wan-Xiang Li; Shou-Wei Ding
Source:
Trends in biochemical sciences 2022 pp. -
ISSN:
0968-0004
Subject:
double-stranded RNA,   etc   ; RNA interference; cell culture; mammals; viruses;     Show all 5 Subjects
Abstract:
... The antiviral defense directed by the RNAi pathway employs distinct specificity and effector mechanisms compared with other immune responses. The specificity of antiviral RNAi is programmed by siRNAs processed from virus-derived double-stranded RNA by Dicer endonuclease. Argonaute-containing RNA-induced silencing complex loaded with the viral siRNAs acts as the effector to mediate specific virus c ...
DOI:
10.1016/j.tibs.2022.05.001

4. New Insights into the Genome Organization of Yeast Double-Stranded RNA LBC Viruses

Author:
Manuel Ramírez; Alberto Martínez; Felipe Molina
Source:
Microorganisms 2022 v.10 no.1 pp. -
ISSN:
2076-2607
Subject:
double-stranded RNA,   etc   ; Saccharomyces cerevisiae; Torulaspora delbrueckii; genome; phenotype; viruses; yeasts;     Show all 7 Subjects
Abstract:
... The yeasts Torulaspora delbrueckii (Td) and Saccharomyces cerevisiae (Sc) may show a killer phenotype that is encoded in dsRNA M viruses (V-M), which require the helper activity of another dsRNA virus (V-LA or V-LBC) for replication. Recently, two TdV-LBCbarr genomes, which share sequence identity with ScV-LBC counterparts, were characterized by high-throughput sequencing (HTS). They also share so ...
DOI:
10.3390/microorganisms10010173

5. Bombyx mori Akirin hijacks a viral peptide vSP27 encoded by BmCPV circRNA and activates the ROS-NF-κB pathway against viral infection

Author:
Yunshan Zhang; Xing Zhang; Kun Dai; Min Zhu; Zi Liang; Jun Pan; Ziyao Zhang; Renyu Xue; Guangli Cao; Xiaolong Hu; Chengliang Gong
Source:
International journal of biological macromolecules 2022 v.194 pp. 223-232
ISSN:
0141-8130
Subject:
double-stranded RNA,   etc   ; Bombyx mori; Cypovirus; antimicrobial peptides; circular RNA; genome; immunity; models;     Show all 8 Subjects
Abstract:
... Bombyx mori cypovirus (BmCPV), a member of the family Reoviridae, is a model of Cypovirus, has a 10 segmented double-stranded RNA genome. However, so far, only one viral small peptide vSP27 with negative regulation on viral infection was identified; the mechanisms underlying host-BmCPV interaction are still unknown. Here, we identified that vSP27 was translated from a BmCPV derived circular RNA (c ...
DOI:
10.1016/j.ijbiomac.2021.11.201

6. Trunk injection delivery of dsRNA for RNAi‐based pest control in apple trees

Author:
John C Wise; Annabel G Wise; Mamy Rakotondravelo; Christine Vandervoort; Candace Seeve; Brad Fabbri
Source:
Pest management science 2022 v.78 no.8 pp. 3528-3533
ISSN:
1526-498X
Subject:
double-stranded RNA,   etc   ; RNA interference; apples; canopy; leaves; reverse transcriptase polymerase chain reaction;     Show all 6 Subjects
Abstract:
... BACKGROUND: RNA interference (RNAi) is a promising new approach for controlling insect pests without the use of synthetic pesticides. Trunk injection is a delivery system for woody plants that harnesses the vascular system of the tree to transport materials to the tree canopy. Full size apple trees were injected with double‐stranded RNA (dsRNA), and season‐long leaf samples were taken to measure t ...
DOI:
10.1002/ps.6993

7. Micropeptide vsp21 translated by Reovirus circular RNA 000048 attenuates viral replication

Author:
Yunshan Zhang; Min Zhu; Xing Zhang; Kun Dai; Zi Liang; Jun Pan; Ziyao Zhang; Manman Cao; Renyu Xue; Guangli Cao; Xiaolong Hu; Chengliang Gong
Source:
International journal of biological macromolecules 2022 v.209 pp. 1179-1187
ISSN:
0141-8130
Subject:
double-stranded RNA,   etc   ; Bombyx mori; Cypovirus; DNA; amino acids; circular RNA; genomics; virus replication;     Show all 8 Subjects
Abstract:
... To date, some DNA viruses and single-stranded RNA viruses have been found to generate circRNAs. However, the reports on circRNAs produced by double-stranded RNA viruses are very limited. In this study, Bombyx mori cypovirus (BmCPV), a typical double-stranded RNA virus belonging to the Reoviridae, was demonstrated to generate viral circRNAs (vcircRNAs) and a vcircRNA_000048 whose sequence correspon ...
DOI:
10.1016/j.ijbiomac.2022.04.136

8. Nanoparticle facilitated stacked-dsRNA improves suppression of the Lepidoperan pest Chilo suppresallis

Author:
Lin Niu; Haixia Yan; Yajie Sun; Delin Zhang; Weihua Ma; Yongjun Lin
Source:
Pesticide biochemistry and physiology 2022 v.187 pp. 105183
ISSN:
0048-3575
Subject:
double-stranded RNA,   etc   ; gene expression; gene targeting; genes; larvae; midgut; mortality; pest control; pesticides;     Show all 9 Subjects
Abstract:
... In recent years, gene knockdown technology using double-stranded RNA (dsRNA) has been widely used as an environment-friendly pest control strategy, but its instability and limited cellular uptake have limited its overall effect. Studies have shown that the efficiency of single dsRNA can be improved by using various nanomaterials. However, the effect of stacked-dsRNA wrapped by nanomaterial on pest ...
DOI:
10.1016/j.pestbp.2022.105183

9. Protection of Double-Stranded RNA via Complexation with Double Hydrophilic Block Copolymers: Influence of Neutral Block Length in Biologically Relevant Environments

Author:
Charlotte E. Pugsley; R. Elwyn Isaac; Nicholas. J. Warren; Juliette S. Behra; Kaat Cappelle; Rosa Dominguez-Espinosa; Olivier. J. Cayre
Source:
Biomacromolecules 2022 v.23 no.6 pp. 2362-2373
ISSN:
1526-4602
Subject:
double-stranded RNA,   etc   ; DNA; agrochemicals; composite polymers; gel electrophoresis; hydrophilicity; ionic strength; polymerization; ribonucleases; therapeutics;     Show all 10 Subjects
Abstract:
... Interaction between the anionic phosphodiester backbone of DNA/RNA and polycations can be exploited as a means of delivering genetic material for therapeutic and agrochemical applications. In this work, quaternized poly(2-(dimethylamino)ethyl methacrylate)-block-poly(N,N-dimethylacrylamide) (PQDMAEMA-b-PDMAₘ) double hydrophilic block copolymers (DHBCs) were synthesized via reversible addition–frag ...
DOI:
10.1021/acs.biomac.2c00136

10. CRISPR guides induce gene silencing in plants in the absence of Cas

Author:
Veerendra Kumar Sharma; Sandeep Marla; Wenguang Zheng; Divya Mishra; Jun Huang; Wei Zhang; Geoffrey Preston Morris; David Edward Cook
Source:
Genome biology 2022 v.23 no.1 pp. 6
ISSN:
1474-760X
Subject:
double-stranded RNA,   etc   ; Arabidopsis; CRISPR-Cas systems; DNA; eukaryotic cells; genes; genomics; immunity; insects; microRNA;     Show all 10 Subjects
Abstract:
... BACKGROUND: RNA-targeting CRISPR-Cas can provide potential advantages over DNA editing, such as avoiding pleiotropic effects of genome editing, providing precise spatiotemporal regulation, and expanded function including antiviral immunity. RESULTS: Here, we report the use of CRISPR-Cas13 in plants to reduce both viral and endogenous RNA. Unexpectedly, we observe that crRNA designed to guide Cas13 ...
DOI:
10.1186/s13059-021-02586-7

11. Silencing of CYP6AS160 in Solenopsis invicta Buren by RNA interference enhances worker susceptibility to fipronil

Author:
Bai-Zhong Zhang; Gui-Lei Hu; Liu-Yang Lu; Xi-Ling Chen; Xi-Wu Gao
Source:
Bulletin of entomological research 2022 v.112 no.2 pp. 171-178
ISSN:
1475-2670
Subject:
double-stranded RNA,   etc   ; RNA interference; Solenopsis invicta; abdomen; cytochrome P-450; fipronil; genes; mortality; pest resistance; research;     Show all 10 Subjects
Abstract:
... Cytochrome P450 monooxygenases play a key role in pest resistance to insecticides by detoxification. Four new P450 genes, CYP6AS160, CYP6AS161, CYP4AB73 and CYP4G232 were identified from Solenopsis invicta. CYP6AS160 was highly expressed in the abdomen and its expression could be induced significantly with exposure to fipronil, whereas CYP4AB73 was not highly expressed in the abdomen and its expre ...
DOI:
10.1017/S0007485321000651

12. Molecularly engineered siRNA conjugates for tumor-targeted RNAi therapy

Author:
Jong Won Lee; Jiwon Choi; Yeonho Choi; Kwangmeyung Kim; Yoosoo Yang; Sun Hwa Kim; Hong Yeol Yoon; Ick Chan Kwon
Source:
Journal of controlled release 2022 pp. -
ISSN:
0168-3659
Subject:
double-stranded RNA,   etc   ; RNA interference; adsorption; chemical bonding; gene expression; genes; liver; nanocarriers; oligonucleotides; peptides; pharmacokinetics; toxicity;     Show all 12 Subjects
Abstract:
... RNA interference (RNAi) is a major cellular mechanism regulating gene expression in which short double-stranded RNA molecules called small interfering RNA (siRNA) mediate sequence-specific mRNA degradation. RNAi technology has recently emerged as a promising therapeutic platform for the effective treatment of various diseases caused by inappropriate gene activity, such as cancer. However, the clin ...
DOI:
10.1016/j.jconrel.2022.09.040

13. RNA circles with minimized immunogenicity as potent PKR inhibitors

Author:
Chu-Xiao Liu; Si-Kun Guo; Fang Nan; Yi-Feng Xu; Li Yang; Ling-Ling Chen
Source:
Molecular cell 2022 v.82 no.2 pp. 420-434.e6
ISSN:
1097-2765
Subject:
double-stranded RNA,   etc   ; Anabaena; RNA ligase (ATP); bacteriophages; exons; immune response; immunogenicity; introns; protein kinases; thymidylate synthase;     Show all 10 Subjects
Abstract:
... Exon back-splicing-generated circular RNAs, as a group, can suppress double-stranded RNA (dsRNA)-activated protein kinase R (PKR) in cells. We have sought to synthesize immunogenicity-free, short dsRNA-containing RNA circles as PKR inhibitors. Here, we report that RNA circles synthesized by permuted self-splicing thymidylate synthase (td) introns from T4 bacteriophage or by Anabaena pre-tRNA group ...
DOI:
10.1016/j.molcel.2021.11.019

14. The Characterization of a Novel Virus Discovered in the Yeast Pichia membranifaciens

Author:
Mark D. Lee; Jack W. Creagh; Lance R. Fredericks; Angela M. Crabtree; Jagdish Suresh Patel; Paul A. Rowley
Source:
Viruses 2022 v.14 no.3 pp. -
ISSN:
1999-4915
Subject:
double-stranded RNA,   etc   ; Pichia membranifaciens; RNA replication; Saccharomyces cerevisiae virus L-A; ancestry; animal diseases; mammals; monophyly; mycoviruses; yeasts;     Show all 10 Subjects
Abstract:
... Mycoviruses are widely distributed across fungi, including the yeasts of the Saccharomycotina subphylum. This manuscript reports the first double-stranded RNA (dsRNA) virus isolated from Pichia membranifaciens. This novel virus has been named Pichia membranifaciens virus L-A (PmV-L-A) and is a member of the Totiviridae. PmV-L-A is 4579 bp in length, with RNA secondary structures similar to the pac ...
DOI:
10.3390/v14030594

15. Molecular Characterization of UDP-N-Acetylglucosamine Pyrophosphorylase and Its Role in the Growth and Development of the White-Backed Planthopper Sogatella furcifera (Hemiptera: Delphacidae)

Author:
Zhao Wang; Gui-Yun Long; Cao Zhou; Dao-Chao Jin; Hong Yang; Wen-Jia Yang
Source:
Genes 2022 v.13 no.8 pp. -
ISSN:
2073-4425
Subject:
double-stranded RNA,   etc   ; Sogatella furcifera; biosynthesis; chitin; enzymes; genes; growth and development; head; integument; mortality; quantitative polymerase chain reaction;     Show all 11 Subjects
Abstract:
... UDP-N-acetylglucosamine pyrophosphorylase (UAP) is a key enzyme in the chitin biosynthesis pathway of insects. Here, we described the gene SfUAP in the white-backed planthopper Sogatella furcifera (Horváth) with an open reading frame of 1470 bp. Quantitative real-time polymerase chain reaction (qPCR) suggested that SfUAP exhibits a different developmental expression pattern and a higher expression ...
DOI:
10.3390/genes13081340

16. Transgenic plants expressing immunosuppressive dsRNA improve entomopathogen efficacy against Spodoptera littoralis larvae

Author:
Ilaria Di Lelio; Eleonora Barra; Mariangela Coppola; Giandomenico Corrado; Rosa Rao; Silvia Caccia
Source:
Journal of pest science 2022 v.95 no.3 pp. 1413-1428
ISSN:
1612-4758
Subject:
double-stranded RNA,   etc   ; Spodoptera littoralis; encapsulation; entomopathogens; food consumption; genes; hemocytes; immune response; immunosuppression; insects; pest control; pests; tobacco;     Show all 13 Subjects
Abstract:
... Transgenic plants that express double-stranded RNA (dsRNA) targeting vital insect genes have recently emerged as a valuable new tool for pest control. In this study, tobacco plants were transformed to produce dsRNA targeting Sl 102 gene that is involved in the immune response of Spodoptera littoralis larvae, a serious lepidopteran pest of several crops. Experimental larvae reared on transgenic tob ...
DOI:
10.1007/s10340-021-01467-z

17. The stability and sequence cleavage preference of dsRNA are key factors differentiating RNAi efficiency between migratory locust and Asian corn borer

Author:
Yunhe Fan; Huifang Song; Mureed Abbas; Yanli Wang; Xiaojian Liu; Tao Li; Enbo Ma; Kun Yan Zhu; Jianzhen Zhang
Source:
Insect biochemistry and molecular biology 2022 v.143 pp. 103738
ISSN:
0965-1748
Subject:
double-stranded RNA,   etc   ; Locusta migratoria; Ostrinia furnacalis; genes; hemolymph; insect biochemistry; insects; integument; midgut; migratory behavior; molecular biology; nucleases;     Show all 12 Subjects
Abstract:
... We compared the stability of double-stranded RNA (dsRNA) in each of two body fluids (hemolymph, midgut fluid) and in each of two tissues (integument, midgut), and the uptake of dsRNA in each of two cultured tissues (integument, midgut) between the migratory locust (Locusta migratoria) and the Asian corn borer (Ostrinia furnacalis). We further compared the abundance of putative small interfering RN ...
DOI:
10.1016/j.ibmb.2022.103738

18. RNAi-Mediated Silencing of Putative Halloween Gene Phantom Affects the Performance of Rice Striped Stem Borer, Chilo suppressalis

Author:
Muhammad Faisal Shahzad; Atif Idrees; Ayesha Afzal; Jamshaid Iqbal; Ziyad Abdul Qadir; Azhar Abbas Khan; Ayat Ullah; Jun Li
Source:
Insects 2022 v.13 no.8 pp. -
ISSN:
2075-4450
Subject:
double-stranded RNA,   etc   ; Chilo suppressalis; biogenesis; biosynthesis; boring insects; cholesterol; ecdysterone; genes; instars; larvae; larval development; molting; mortality; phylogeny; rice;     Show all 15 Subjects
Abstract:
... The physiological and biochemical characterization of the “Halloween” genes has fundamental importance in the biosynthesis pathway of ecdysteroids. These genes were found to catalyze the final phases of ecdysteroid biosynthesis from dietary cholesterol to the molting hormone 20-hydroxyecdysone. We report the characterization of the Cs-Phm in a major insect pest in agriculture, the rice striped ste ...
DOI:
10.3390/insects13080731

19. Characterization of a Novel Double-Stranded RNA Virus from Phytophthora pluvialis in New Zealand

Author:
Zhi Xu; Mahmoud E. Khalifa; Rebekah A. Frampton; Grant R. Smith; Rebecca L. McDougal; Robin M. MacDiarmid; Falk Kalamorz
Source:
Viruses 2022 v.14 no.2 pp. -
ISSN:
1999-4915
Subject:
double-stranded RNA,   etc   ; Phytophthora; RNA-directed RNA polymerase; amino acids; diagnostic techniques; genome; genotype; geographical distribution; phylogeny; prediction; viruses; New Zealand;     Show all 12 Subjects
Abstract:
... A new dsRNA virus from the oomycete Phytophthora pluvialis has been characterized and designated as Phytophthora pluvialis RNA virus 1 (PplRV1). The genome of the PplRV1 reference genome is 6742 bp that encodes two predicted open reading frames (ORFs). ORF1 and ORF2 overlap by a 47 nt “slippery” frameshift sequence. ORF1 encodes a putative protein of unknown function. ORF2 shows high similarity to ...
DOI:
10.3390/v14020247

20. Impaired Antiviral Responses to Extracellular Double-Stranded RNA and Cytosolic DNA, but Not to Interferon-α Stimulation, in TRIM56-Deficient Cells

Author:
Dang Wang; Ruixue Wang; Kui Li
Source:
Viruses 2022 v.14 no.1 pp. -
ISSN:
1999-4915
Subject:
double-stranded RNA,   etc   ; DNA; Toll-like receptor 3; antiviral properties; gene targeting; genes; humans; innate immunity; ubiquitin-protein ligase; vesicular stomatitis;     Show all 10 Subjects
Abstract:
... The physiologic function of tripartite motif protein 56 (TRIM56), a ubiquitously expressed E3 ligase classified within the large TRIM protein family, remains elusive. Gene knockdown studies have suggested TRIM56 as a positive regulator of the type I interferon (IFN-I) antiviral response elicited via the Toll-like receptor 3 (TLR3) and cyclic GMP–AMP synthase (cGAS)–stimulator of interferon genes ( ...
DOI:
10.3390/v14010089