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1. Using two confluent capillary columns for improved gas chromatography‐electroantennographic detection (GC‐EAD)

Author:
Shuttleworth, Adam; Johnson, Steven D.
Source:
Entomologia experimentalis et applicata 2020 v.168 no.2 pp. 191-197
ISSN:
0013-8703
Subject:
electroantennography,   etc   ; active ingredients; gas chromatography; micellar electrokinetic capillary chromatography;     Show all 4 Subjects
Abstract:
... We discuss the modification of an old method of connecting two capillary columns in a confluent configuration which elegantly allows introduction of make‐up gas at a controlled flow rate when the effluent is split for electroantennographic detection experiments, and allows for easy switching of the stationary phase between successive GC runs. Being able to run samples on different phases is extrem ...
DOI:
10.1111/eea.12873

2. CcOBP2 plays a crucial role in 3-carene olfactory response of the parasitoid wasp Chouioia cunea

Author:
Pan, Lina; Xiang, Weifang; Sun, Zeyang; Yang, Yixin; Han, Jiayi; Wang, Yonghui; Yan, Chuncai; Li, Min
Source:
Insect biochemistry and molecular biology 2020 v.117 pp. 103286
ISSN:
0965-1748
Subject:
electroantennography,   etc   ; amino acids; antennae; carene; computer simulation; fluorescence; insects; natural enemies; parasitic wasps; pupae; semiochemicals; signal transduction; volatile compounds;     Show all 13 Subjects
Abstract:
... Chouioia cunea (Yang) is a pupal parasitoid wasp and this species is able to seek host insects depending on its olfactory system. However, the molecular mechanism of the olfactory system in the C. cunea is still limited. To identify putative semiochemicals bound to CcOBP2, a protein specifically expressed in antennae, 14 compounds from the pupae of H. cunea and 11 common volatile compounds from pl ...
DOI:
10.1016/j.ibmb.2019.103286

3. Presence and roles of myrtenol, myrtanol and myrtenal in Dendroctonus armandi (Coleoptera: Curculionidae: Scolytinae) and Pinus armandi (Pinales: Pinaceae: Pinoideae)

Author:
Zhao, Mingzhen; Liu, Bin; Sun, Yaya; Wang, Yuanyuan; Dai, Lulu; Chen, Hui
Source:
Pest management science 2020 v.76 no.1 pp. 188-197
ISSN:
1526-498X
Subject:
electroantennography,   etc   ; Curculionidae; Dendroctonus; Pinus; aggregation pheromones; females; gas chromatography-mass spectrometry; insect pheromones; pest control; phloem; pollution; toxicity; trees;     Show all 13 Subjects
Abstract:
... BACKGROUND: Insect pheromones and host volatiles are important for pest control due to their high efficiency and low potential for environmental pollution. The functions of myrtenol, myrtanol and myrtenal in pest–host interactions are unknown. This study aimed to determine the presence of myrtenol, myrtanol and myrtenal in newly emerged and emerged stages of Dendroctonus armandi, and in infected a ...
DOI:
10.1002/ps.5492

4. Conflicting selection on floral scent emission in the orchid Gymnadenia conopsea

Author:
Chapurlat, Elodie; Ågren, Jon; Anderson, Joseph; Friberg, Magne; Sletvold, Nina
Source:
Thenew phytologist 2019 v.222 no.4 pp. 2009-2022
ISSN:
0028-646X
Subject:
electroantennography,   etc   ; Gymnadenia conopsea; evolution; flowering; odors; pollinators; reproductive success;     Show all 7 Subjects
Abstract:
... Floral scent is a crucial trait for pollinator attraction. Yet only a handful of studies have estimated selection on scent in natural populations and no study has quantified the relative importance of pollinators and other agents of selection. In the fragrant orchid Gymnadenia conopsea, we used electroantennographic data to identify floral scent compounds detected by local pollinators and quantifi ...
DOI:
10.1111/nph.15747

5. Detecting Host-Plant Volatiles with Odorant Receptors from Grapholita molesta (Busck) (Lepidoptera: Tortricidae)

Author:
Chen, Lihui; Tian, Ke; Xu, Xiangli; Fang, Aisheng; Cheng, Weining; Wang, Guirong; Liu, Wei; Wu, Junxiang
Source:
Journal of agricultural and food chemistry 2020 v.68 no.9 pp. 2711-2717
ISSN:
1520-5118
Subject:
electroantennography,   etc   ; Grapholita molesta; Xenopus; adults; aldehydes; antennae; females; genes; host plants; insect control; insects; males; odor compounds; odorant receptors; oocytes; pests; pome fruits; quantitative polymerase chain reaction;     Show all 18 Subjects
Abstract:
... Grapholita molesta is a global pest of stone and pome fruits. The sensitive olfactory system plays a crucial role in regulating key behavioral activities of insects and G. molesta relies heavily on general odorant receptors (ORs) to detect host-plant volatiles. In this study, three general OR genes from G. molesta (GmolOR12, GmolOR20, and GmolOR21) were identified. Quantitative polymerase chain re ...
DOI:
10.1021/acs.jafc.9b07305

6. Functional characterization of one sex pheromone receptor (AlucOR4) in Apolygus lucorum (Meyer-Dür)

Author:
An, Xingkui; Khashaveh, Adel; Liu, Danfeng; Xiao, Yong; Wang, Qi; Wang, Shanning; Geng, Ting; Gu, Shaohua; Zhang, Yongjun
Source:
Journal of insect physiology 2020 v.120 pp. 103986
ISSN:
0022-1910
Subject:
electroantennography,   etc   ; Xenopus; adults; antennae; bait traps; butyrates; field experimentation; insect physiology; males; messenger RNA; monitoring; odorant receptors; oocytes; quantitative polymerase chain reaction; sex pheromones;     Show all 15 Subjects
Abstract:
... Traps baited with female-produced sex pheromones have been very effective in the monitoring and management of mirid bugs in numerous field trials. However, none of the target odorant receptors for sex pheromone components in Apolygus lucorum have been identified. Here, we identified one candidate sex pheromone receptor, AlucOR4, from A. lucorum. Quantitative real-time PCR (qPCR) analysis revealed ...
DOI:
10.1016/j.jinsphys.2019.103986

7. Reduced effectiveness of repellents in a pyrethroid‐resistant strain of Aedes aegypti (Diptera: culicidae) and its correlation with olfactory sensitivity

Author:
Yang, Liu; Norris, Edmund J; Jiang, Shiyao; Bernier, Ulrich R; Linthicum, Kenneth J; Bloomquist, Jeffrey R
Source:
Pest management science 2020 v.76 no.1 pp. 118-124
ISSN:
1526-498X
Subject:
electroantennography,   etc   ; Aedes aegypti; deet; dengue; electrophysiology; evolution; insect repellents; mutation; pest management; pesticide resistance; pyrethrins; vapors; viruses; yellow fever; Puerto Rico;     Show all 15 Subjects
Abstract:
... BACKGROUND: The mosquito, Aedes aegypti (Diptera: Culicidae), is a vector of dengue fever, zika, chikungunya, and yellow fever viruses, and in many areas possesses significant levels of resistance to pyrethroids. Behavioral performance was assessed in 15, 30, and 60 min exposures in a high throughput vapor phase spatial repellency assay to three contact repellent standards: N,N‐diethyl‐3‐methylben ...
DOI:
10.1002/ps.5562

8. Role of chemical cues in cabbage stink bug host plant selection

Author:
Piersanti, Silvana; Rebora, Manuela; Ederli, Luisa; Pasqualini, Stefania; Salerno, Gianandrea
Source:
Journal of insect physiology 2020 v.120 pp. 103994
ISSN:
0022-1910
Subject:
electroantennography,   etc   ; Brassica oleracea; Eruca vesicaria subsp. sativa; Eurydema; adults; broccoli; cabbage; cole crops; host plants; insect physiology; pests; sensilla; smell; trap crops; volatile organic compounds; Europe;     Show all 16 Subjects
Abstract:
... The cabbage stink bugs of the genus Eurydema, encompassing several oligophagous species, such as Eurydema oleracea (L.), are known to be important pests of cabbage, broccoli, and other cole crops in Europe. Despite their economic importance, the knowledge regarding the role of chemical cues in host plant selection of these species is very limited. The present investigation on E. oleracea at the ad ...
DOI:
10.1016/j.jinsphys.2019.103994

9. (S)-2-Heptanol, the alarm pheromone of the stingless bee Melipona solani (Hymenoptera, Meliponini)

Author:
Alavez-Rosas, David; Sánchez-Guillén, Daniel; Malo, Edi A.; Cruz-López, Leopoldo
Source:
Apidologie 2019 v.50 no.3 pp. 277-287
ISSN:
0044-8435
Subject:
electroantennography,   etc   ; Melipona solani; active ingredients; alarm pheromones; bioassays; electrophysiology; flight; mandibular glands; optical isomerism; stingless bees;     Show all 10 Subjects
Abstract:
... Timely defence response is critical in any highly social bee species. Chemical signalling is closely linked to this behaviour, and several pheromones have been discovered. In this work, we identified the pheromone of the stingless bee Melipona solani and evaluated the electrophysiological and behavioural response of the bee to the identified compound. We determined that the mandibular glands serve ...
DOI:
10.1007/s13592-019-00639-2

10. Early Olfactory Environment Influences Antennal Sensitivity and Choice of the Host-Plant Complex in a Parasitoid Wasp

Author:
Luquet, Martin; Tritto, Olympe; Cortesero, Anne-Marie; Jaloux, Bruno; Anton, Sylvia
Source:
Insects 2019 v.10 no.5
ISSN:
2075-4450
Subject:
electroantennography,   etc   ; Aphidius ervi; biological control; cropping systems; females; foraging; host plants; olfactometers; olfactory perception; parasitic wasps;     Show all 10 Subjects
Abstract:
... Early experience of olfactory stimuli associated with their host–plant complex (HPC) is an important driver of parasitoid foraging choices, notably leading to host fidelity. Mechanisms involved, such as peripheral or central modulation, and the impact of a complex olfactory environment are unknown. Using olfactometer assays, we compared HPC preference of Aphidius ervi Haliday (Hymenoptera:Br ...
DOI:
10.3390/insects10050127
PubMed:
31058845
PubMed Central:
PMC6571609

11. Identification of Aggregation-Sex Pheromone Components for a “Living Fossil”, the False Click Beetle, Palaeoxenus dohrni Horn (Coleoptera: Eucnemidae)

Author:
Serrano, Jacqueline M.; McElfresh, J. Steven; Zou, Yunfan; Millar, Jocelyn G.
Source:
Journal of chemical ecology 2019 v.45 no.4 pp. 366-370
ISSN:
0098-0331
Subject:
electroantennography,   etc   ; Coleoptera; adults; antennae; attractants; bioassays; endangered species; headspace analysis; insect pheromones; surveys; California;     Show all 11 Subjects
Abstract:
... Insect pheromones have rarely been exploited in surveys or studies of rare and endangered species, despite their potential as effective and highly selective attractants for target species. Here, we report the identification, synthesis, and field bioassays of a male-produced aggregation-sex pheromone blend of a rare false click beetle species endemic to southern California, Dohrn’s elegant eucnemid ...
DOI:
10.1007/s10886-019-01068-2

12. Identification of Δ6‐unsaturated, monoenyl type I pheromone compounds from the cashew stem borer Anthistarcha binocularis (Lepidoptera: Gelechiidae)

Author:
Leal Soares, Ariane M; B França, Paulo H; Triana, Merybeth F; dos Santos, Jakeline M; S Dias‐Pini, Nívia; F Goulart, Henrique; Araújo‐Júnior, João X; Goulart Santana, Antônio E
Source:
Pest management science 2020 v.76 no.4 pp. 1435-1442
ISSN:
1526-498X
Subject:
electroantennography,   etc   ; Gelechiidae; acetates; alcohols; antennae; bioactive compounds; boring insects; chemical control; electrophysiology; endophytes; females; field experimentation; financial economics; gas chromatography-mass spectrometry; insect larvae; males; monitoring; orchards; pest management; sex pheromones; stereoisomers; Brazil;     Show all 22 Subjects
Abstract:
... BACKGROUND: The cashew stem borer Anthistarcha binocularis (Meyrick) is a major pest in cashew orchards in Brazil. The damage caused by the larvae results in economic losses, and the available chemical control is not suitable because of the endophytic nature of A. binocularis. The identification of the A. binocularis sex pheromone could provide novel applications for the detection and monitoring o ...
DOI:
10.1002/ps.5656

13. Identification of odorant-binding proteins and functional analysis of antenna-specific AplaOBP1 in the emerald ash borer, Agrilus planipennis

Author:
Wang, Shan-Ning; Shan, Shuang; Yu, Guo-Yue; Wang, He; Dhiloo, Khalid Hussain; Khashaveh, Adel; Zhang, Fan; Zhang, Yong-Jun
Source:
Journal of pest science 2020 v.93 no.2 pp. 853-865
ISSN:
1612-4758
Subject:
electroantennography,   etc   ; Agrilus planipennis; antennae; binding capacity; electrophysiology; fluorescence; genes; immunocytochemistry; in situ hybridization; ligands; limonene; lymph; myrcene; nerolidol; ocimene; odorant receptors; olfactory perception; phylogeny; reverse transcriptase polymerase chain reaction; semiochemicals; sensilla; smell; transcriptome;     Show all 23 Subjects
Abstract:
... Olfaction-based strategies have been successfully applied to monitor the emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae); however, roles of chemosensory-associated proteins in olfactory perception of A. planipennis are rarely reported. Here, we identified 11 odorant-binding proteins (OBPs) from the A. planipennis transcriptome and genome. Sequence and phylogenetic analyse ...
DOI:
10.1007/s10340-019-01188-4

14. Pyrethroid-Derived Acids and Alcohols: Bioactivity and Synergistic Effects on Mosquito Repellency and Toxicity

Author:
Yang, Liu; Richoux, Gary M.; Norris, Edmund J.; Cuba, Ingeborg; Jiang, Shiyao; Coquerel, Quentin; Demares, Fabien; Linthicum, Kenneth J.; Bloomquist, Jeffrey R.
Source:
Journal of agricultural and food chemistry 2020 v.68 no.10 pp. 3061-3070
ISSN:
1520-5118
Subject:
electroantennography,   etc   ; Aedes aegypti; Drosophila melanogaster; acids; alcohols; antennae; cross resistance; deet; electrophysiology; esterases; insect larvae; insecticides; mechanism of action; mutation; neurons; pyrethrins; sodium channels; synergism; toxicity; vapors; Puerto Rico;     Show all 21 Subjects
Abstract:
... Pyrethroids are one of the most commonly used classes of insecticides, and their acid and alcohol components are esterase degradation products, usually considered to be biologically inactive. In this study, it was found that several pyrethroid acids had a spatial repellent activity that was greater than DEET, often more active than the parent pyrethroids, and showed little cross resistance in a py ...
DOI:
10.1021/acs.jafc.9b07979

15. Diel rhythm of volatile emissions of males and females of the peach fruit fly Bactrocera zonata

Author:
Levi-Zada, A.; Levy, A.; Rempoulakis, P.; Fefer, D.; Steiner, S.; Gazit, Y.; Nestel, D.; Yuval, B.; Byers, J.A.
Source:
Journal of insect physiology 2020 v.120 pp. 103970
ISSN:
0022-1910
Subject:
electroantennography,   etc   ; Bactrocera zonata; antennae; automation; dose response; emissions; esters; females; fruit flies; fruits; gas chromatography-mass spectrometry; headspace analysis; insect pests; insect physiology; males; peaches; periodicity; pheromones; pyrazines; solid phase microextraction; solvents; vegetables; volatile compounds;     Show all 23 Subjects
Abstract:
... Fruit flies in the genus Bactrocera are among the most destructive insect pests of fruits and vegetables throughout the world. A number of studies have identified volatiles from fruit flies, but few reports have demonstrated behavioral effects or sensitivities of fly antennae to these compounds. We applied a recently developed method of automated headspace analysis using SPME (Solid Phase Microext ...
DOI:
10.1016/j.jinsphys.2019.103970

16. Evaluation of pheromone of false codling moth Thaumatotibia leucotreta in Israel by sequential SPME/GCMS analysis and field trials

Author:
Levi-Zada, Anat; Fefer, Daniela; Madar, Reut; Steiner, Sara; Kaspi, Roy
Source:
Journal of pest science 2020 v.93 no.1 pp. 519-529
ISSN:
1612-4758
Subject:
electroantennography,   etc   ; Citrus; Thaumatotibia leucotreta; crops; eclosion; females; field experimentation; fruit trees; gas chromatography-mass spectrometry; geographical distribution; insects; males; monitoring; pests; pheromone glands; pheromone traps; pheromones; polyethylene; solid phase microextraction; Israel; Sub-Saharan Africa;     Show all 21 Subjects
Abstract:
... The false codling moth (FCM), Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae), is an indigenous pest of citrus and other crops in sub-Saharan areas of Africa. The pest was first recorded in Israel in the 1980s and has remained at low population levels although extending its geographic range. The pest is difficult to detect without pheromone traps, but published information is conflic ...
DOI:
10.1007/s10340-019-01138-0

17. Host plant preferences and detection of host plant volatiles of the migrating psyllid species Cacopsylla pruni, the vector of European Stone Fruit Yellows

Author:
Gallinger, Jannicke; Jarausch, Barbara; Jarausch, Wolfgang; Gross, Jürgen
Source:
Journal of pest science 2020 v.93 no.1 pp. 461-475
ISSN:
1612-4758
Subject:
electroantennography,   etc   ; Cacopsylla; Candidatus Phytoplasma prunorum; Prunus spinosa; conifers; developmental stages; females; gas chromatography; herbivores; host plants; host preferences; insect reproduction; insects; mass spectrometry; migratory behavior; overwintering; plant pathogens; plant-insect relations; plums; rootstocks; volatile organic compounds;     Show all 21 Subjects
Abstract:
... Plant-emitted volatile organic compounds play an important role in plant–insect interactions. Thanks to plant-emitted volatiles, herbivores are able to find suitable hosts. Recognition and location of host plants are a key challenge for successful survival and reproduction of migrating insects, such as the plum psyllid Cacopsylla pruni. This psyllid migrates between Prunus spp. for reproduction an ...
DOI:
10.1007/s10340-019-01135-3

18. Optimizing the attractiveness of pheromone baits used for trapping the four‐eyed spruce bark beetle Polygraphus poligraphus

Author:
Viklund, Lina; Rahmani, Rizan; Bång, Joakim; Schroeder, Martin; Hedenström, Erik
Source:
Journal of applied entomology 2019 v.143 no.7 pp. 721-730
ISSN:
0931-2048
Subject:
electroantennography,   etc   ; Picea; Polygraphus; alpha-terpineol; attractants; bark beetles; forest damage; forests; pheromone traps; pheromones; repellents; synergism; trapping; Sweden;     Show all 14 Subjects
Abstract:
... Bark beetles have caused extensive damage to forests in central Sweden during the past decade, and the four‐eyed spruce bark beetle, Polygraphus poligraphus, seems to be involved. However, its role in these bark beetle outbreaks is still not clear. The purpose of this study was to develop an efficient pheromone bait for P. poligraphus, which would make it possible to study the species more careful ...
DOI:
10.1111/jen.12641

19. Electroantennographic responses and field attraction of an emerging invader, the red-necked longicorn beetle Aromia bungii (Coleoptera: Cerambycidae), to the chiral and racemic forms of its male-produced aggregation-sex pheromone

Author:
Yasui, Hiroe; Fujiwara-Tsujii, Nao; Yasuda, Tetsuya; Fukaya, Midori; Kiriyama, Satoshi; Nakano, Akio; Watanabe, Takahito; Mori, Kenji
Source:
Applied entomology and zoology 2019 v.54 no.1 pp. 109-114
ISSN:
0003-6862
Subject:
electroantennography,   etc   ; Cerambycidae; antennae; apricots; cherries; enantiomers; females; field experimentation; males; peaches; pheromones; plant pests; prunes; trees; Japan;     Show all 15 Subjects
Abstract:
... The red-necked longicorn beetle Aromia bungii (Faldermann) (Coleoptera: Cerambycidae) is an emerging invader in Japan and has become a major pest of cherry blossom trees, peaches, prunes and Japanese apricots. The male-produced aggregation-sex pheromone, recently identified as (E)-2-cis-6,7-epoxynonenal, has two enantiomers. Here, we determined which enantiomer the male produces and tested the res ...
DOI:
10.1007/s13355-018-0600-x

20. Repellent action and contact toxicity mechanisms of the essential oil extracted from Chinese chive against Plutella xylostella larvae

Author:
Gao, Quan; Song, Li; Sun, Jia; Cao, Hai‐Qun; Wang, Likun; Lin, Huafeng; Tang, Feng
Source:
Archives of insect biochemistry and physiology 2019 v.100 no.1 pp. e21509
ISSN:
0739-4462
Subject:
electroantennography,   etc   ; Allium tuberosum; Plutella xylostella; acetylcholinesterase; active ingredients; botanical pesticides; carboxylesterase; essential oils; glutathione transferase; larvae; pests; pyrazines; toxicity;     Show all 13 Subjects
Abstract:
... Botanical pesticides play increasingly important roles in the control of agricultural pests. In this study, the insecticidal effects, specifically the repellent action and contact toxicity, of the essential oil extracted from Chinese chive (EOC) against Plutella xylostella larvae were confirmed. The mechanisms of repellent’s action were studied using electroantennograms (EAGs), and the effects on ...
DOI:
10.1002/arch.21509