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1. In situ fixation and subsequent collection of cultured endothelial cells in a shear flow

Author:
Aldrich, Jessica L.; Long, David S.
Source:
MethodsX 2019 v.6 pp. 1164-1173
ISSN:
2215-0161
Subject:
endothelial cells,   etc   ; chromatin; dissociation;     Show all 3 Subjects
Abstract:
... In situ fixation of adherent cells is a necessary process for downstream assays. Current methods to dissociate adherent endothelial cells require the use of a cell scraper that may introduce variability in nuclear morphology. Also, a cell scraper is not an option for experiments using sealed flow chambers. HMEC-1 cells were sheared at 5 dyn/cm² for 24 h and then fixed in situ, quenched, and dissoc ...
DOI:
10.1016/j.mex.2019.05.001
PubMed:
31193472
PubMed Central:
PMC6531827

2. A chitosan modified asymmetric small-diameter vascular graft with anti-thrombotic and anti-bacterial functions for vascular tissue engineering

Author:
Wang, Yilin; He, Chao; Feng, Yunbo; Yang, Ye; Wei, Zhiwei; Zhao, Weifeng; Zhao, Changsheng
Source:
Journal of materials chemistry B 2020 v.8 no.3 pp. 568-577
ISSN:
2050-7518
Subject:
endothelial cells,   etc   ; biocompatibility; chitosan; lactones; mechanical properties; thrombosis; tissue engineering;     Show all 7 Subjects
Abstract:
... Rapid endothelialization and prevention of restenosis are two vital challenges for the preparation of a small-diameter vascular graft (SDVG), while postoperative infection after implantation is often neglected. In the present study, carboxymethyl chitosan (CMC) and chitosan (CS) were chosen as the anti-thrombotic and anti-bacterial components, respectively; and then an asymmetric vascular graft wa ...
DOI:
10.1039/c9tb01755k

3. A novel biofunctionalizing peptide for metallic alloy

Author:
Sakaguchi-Mikami, Akane; Fujimoto, Kazuhiro; Taguchi, Tetsushi; Isao, Karube; Yamazaki, Tomohiko
Source:
Biotechnology letters 2020 v.42 no.5 pp. 747-756
ISSN:
0141-5492
Subject:
endothelial cells,   etc   ; antibodies; biocompatibility; biocompatible materials; myocytes; peptides; phages; smooth muscle; stainless steel; thrombosis; toxicity;     Show all 11 Subjects
Abstract:
... OBJECTIVES: Improving biocompatibility of metallic alloy biomaterials has been of great interest to prevent implant associated-diseases, such as stent thrombosis. Herein a simple and efficient procedure was designed to biofunctionalize a biomaterial surface by isolating a SUS316L stainless steel binding peptide. RESULTS: After three rounds of phage panning procedure, 12 mer peptide (SBP-A; VQHNTKY ...
DOI:
10.1007/s10529-020-02832-1

4. Phase angle could be a marker of microvascular damage in systemic sclerosis

Author:
Gigante, Antonietta; Gasperini, Maria Ludovica; Rosato, Edoardo; Navarini, Luca; Margiotta, Domenico; Afeltra, Antonella; Muscaritoli, Maurizio
Source:
Nutrition 2020 v.73 pp. 110730
ISSN:
0899-9007
Subject:
endothelial cells,   etc   ; autoimmune diseases; disease severity; females; fibrosis; males; patients; sclerosis; vascular endothelial growth factors;     Show all 9 Subjects
Abstract:
... Systemic sclerosis (SSc) is an autoimmune disease characterized by endothelial dysfunction with fibrosis of skin and internal organs. Integrity of the endothelial cell is important to its physiologic function such as production of angiogenetic factors. The aim of this study was to assess whether phase angle (PhA) is altered in patients with SSc and whether its values correlate with vascular endoth ...
DOI:
10.1016/j.nut.2020.110730

5. Skeleton binding protein 1 (SBP1) of Plasmodium falciparum accumulates in electron-dense material before passing through the parasitophorous vacuole membrane

Author:
Iriko, Hideyuki; Ishino, Tomoko; Tachibana, Mayumi; Omoda, Ayaka; Torii, Motomi; Tsuboi, Takafumi
Source:
Parasitology international 2020 v.75 pp. 102003
ISSN:
1383-5769
Subject:
endothelial cells,   etc   ; Plasmodium falciparum; antibodies; binding proteins; erythrocytes; immunization; microscopy; parasites; vacuoles;     Show all 9 Subjects
Abstract:
... Plasmodium falciparum proteins involved in vascular endothelial cell adherence are transported to the surface of infected erythrocytes. These proteins are exported through parasite-derived membrane structures within the erythrocyte cytoplasm called Maurer's clefts. Skeleton binding protein 1 (SBP1) is localized in the Maurer's clefts and plays an important role in transporting molecules to the sur ...
DOI:
10.1016/j.parint.2019.102003

6. A 3D polydimethylsiloxane microhourglass-shaped channel array made by reflowing photoresist structures for engineering a blood capillary network

Author:
Rho, Hoon Suk; Veltkamp, Henk-Willem; Baptista, Danielle; Gardeniers, Han; Le Gac, Séverine; Habibović, Pamela
Source:
Methods 2020
ISSN:
1046-2023
Subject:
endothelial cells,   etc   ; blood; blood vessels; cost effectiveness; drugs; engineering; humans; lungs; microstructure; polydimethylsiloxane; screening; vascular diseases;     Show all 12 Subjects
Abstract:
... This paper describes an innovative yet straightforward fabrication technique to create three-dimensional microstructures with controllable tapered geometries by combining conventional photolithography and thermal reflow of photoresist. Positive photoresist-based microchannel structures with varying width-to-length ratios were reflowed after their fabrication to generate three-dimensional funnel st ...
DOI:
10.1016/j.ymeth.2020.03.007

7. Effect of Ni ion release on the cells in contact with NiTi alloys

Author:
Veverkova, Jana; Bartkova, Denisa; Weiser, Adam; Dlouhy, Antonin; Babula, Petr; Stepka, Petr; Pavkova Goldbergova, Monika
Source:
Environmental science and pollution research international 2020 v.27 no.8 pp. 7934-7942
ISSN:
0944-1344
Subject:
human umbilical vein endothelial cells,   etc   ; alloys; biocompatibility; heat treatment; hydrogen; ions; models; nickel;     Show all 8 Subjects
Abstract:
... Nickel-titanium alloys have been used in medical applications for several years; however, biocompatibility of the material remains controversial. In the present study, the human umbilical vein endothelial cells (HUVEC) were cultured in contact with the nitinol used in two different heat treatment surface modifications—helium and hydrogen. The amount of Ni ions released from these alloys in contact ...
DOI:
10.1007/s11356-019-07506-8

8. Stab2-Mediated Clearance of Supramolecular Polymer Nanoparticles in Zebrafish Embryos

Author:
Saez Talens, Victorio; Arias-Alpizar, Gabriela; Makurat, D. M. M.; Davis, Joyal; Bussmann, Jeroen; Kros, Alexander; Kieltyka, Roxanne E.
Source:
Biomacromolecules 2020 v.21 no.3 pp. 1060-1068
ISSN:
1526-4602
Subject:
endothelial cells,   etc   ; Danio rerio; caudal vein; drugs; embryo (animal); fluorescence; liver; macrophages; nanocarriers; nanoparticles; polymers;     Show all 11 Subjects
Abstract:
... Supramolecular polymers are attractive scaffolds for use as nanocarriers in drug delivery thanks to their modularity and easy fabrication; however, a molecular view into their in vivo behavior is lacking. Herein, we prepare fluorescent squaramide-based supramolecular polymer nanoparticles that range from fibers to spheres while maintaining their surface chemistry and near-neutral surface charge by ...
DOI:
10.1021/acs.biomac.9b01318

9. Hhex regulates the specification and growth of the hepatopancreatic ductal system

Author:
Villasenor, Alethia; Gauvrit, Sébastien; Collins, Michelle M.; Maischein, Hans-Martin; Stainier, Didier Y.R.
Source:
Developmental biology 2020 v.458 no.2 pp. 228-236
ISSN:
0012-1606
Subject:
endothelial cells,   etc   ; Danio rerio; bile ducts; blood vessels; endoderm; transcription factors;     Show all 6 Subjects
Abstract:
... Significant efforts have advanced our understanding of foregut-derived organ development; however, little is known about the molecular mechanisms that underlie the formation of the hepatopancreatic ductal (HPD) system. Here, we report a role for the homeodomain transcription factor Hhex in directing HPD progenitor specification in zebrafish. Loss of Hhex function results in impaired HPD system for ...
DOI:
10.1016/j.ydbio.2019.10.021

10. Complement fragments are biomarkers of antibody-mediated endothelial injury

Author:
Stites, Erik; Renner, Brandon; Laskowski, Jennifer; Le Quintrec, Moglie; You, Zhiying; Freed, Brian; Cooper, James; Jalal, Diana; Thurman, Joshua M.
Source:
Molecular immunology 2020 v.118 pp. 142-152
ISSN:
0161-5890
Subject:
endothelial cells,   etc   ; allografting; antibodies; barium; biomarkers; biopsy; blood serum; complement; humans; kidney transplant; kidneys; models; patients;     Show all 13 Subjects
Abstract:
... Antibody-mediated rejection (AbMR) adversely affects long-term graft survival in kidney transplantation. Currently, the diagnosis of AbMR requires a kidney biopsy, and detection of complement C4d deposition in the allograft is one of the diagnostic criteria. Complement activation also generates several soluble fragments which could potentially provide non-invasive biomarkers of the process. Furthe ...
DOI:
10.1016/j.molimm.2019.12.011
PubMed:
31884386
PubMed Central:
PMC7192552

11. Synchronized tissue-scale vasculogenesis and ubiquitous lateral sprouting underlie the unique architecture of the choriocapillaris

Author:
Ali, Zaheer; Cui, Dongmei; Yang, Yunlong; Tracey-White, Dhani; Vazquez-Rodriguez, Gabriela; Moosajee, Mariya; Ju, Rong; Li, Xuri; Cao, Yihai; Jensen, Lasse D.
Source:
Developmental biology 2020 v.457 no.2 pp. 206-214
ISSN:
0012-1606
Subject:
endothelial cells,   etc   ; Danio rerio; angiogenesis; mice; models; nutrients; retina; sclera; sprouting; vision; wastes;     Show all 11 Subjects
Abstract:
... The choriocapillaris is an exceptionally high density, two-dimensional, sheet-like capillary network, characterized by the highest exchange rate of nutrients for waste products per area in the organism. These unique morphological and physiological features are critical for supporting the extreme metabolic requirements of the outer retina needed for vision. The developmental mechanisms and processe ...
DOI:
10.1016/j.ydbio.2019.02.002

12. MLCK and ROCK mutualism in endothelial barrier dysfunction

Author:
Kazakova, Olga A.; Khapchaev, Asker Y.; Shirinsky, Vladimir P.
Source:
Biochimie 2020 v.168 pp. 83-91
ISSN:
0300-9084
Subject:
endothelial cells,   etc   ; antagonists; edema; mutualism; myosin light chain kinase; myosin light chains; phosphorylation; thrombin; tissues;     Show all 9 Subjects
Abstract:
... Myosin activation contributes to the contractile forces that induce disturbances in the vascular endothelial integrity and promote protein-rich edema of the underlying tissues. Myosin light chain kinase (MLCK) and Rho-associated protein kinase (ROCK) have been reported to phosphorylate myosin regulatory light chains (RLC) for myosin activation. However, the relative contribution and roles of these ...
DOI:
10.1016/j.biochi.2019.10.010

13. Lung endothelial cells are sensitive to epsilon toxin from Clostridium perfringens

Author:
Dorca-Arévalo, Jonatan; Dorca, Eduard; Torrejón-Escribano, Benjamín; Blanch, Marta; Martín-Satué, Mireia; Blasi, Juan
Source:
Veterinary research 2020 v.51 no.1 pp. 27
ISSN:
1297-9716
Subject:
endothelial cells,   etc   ; Clostridium perfringens; brain; cytotoxicity; dogs; edema; endothelium; epithelial cells; kidneys; lungs; messenger RNA; models; myelin sheath; oligomerization; poisoning;     Show all 15 Subjects
Abstract:
... The pore-forming protein epsilon toxin (Etx) from Clostridium perfringens produces acute perivascular edema affecting several organs, especially the brain and lungs. Despite the toxin evident effect on microvasculature and endothelial cells, the underlying molecular and cellular mechanisms remain obscure. Moreover, no Etx-sensitive endothelial cell model has been identified to date. Here, we chara ...
DOI:
10.1186/s13567-020-00748-2
PubMed:
32093740
PubMed Central:
PMC7041264

14. Senescence-Induced Vascular Remodeling Creates Therapeutic Vulnerabilities in Pancreas Cancer

Author:
Ruscetti, Marcus; Morris, John P.; Mezzadra, Riccardo; Russell, James; Leibold, Josef; Romesser, Paul B.; Simon, Janelle; Kulick, Amanda; Ho, Yu-jui; Fennell, Myles; Li, Jinyang; Norgard, Robert J.; Wilkinson, John E.; Alonso-Curbelo, Direna; Sridharan, Ramya; Heller, Daniel A.; de Stanchina, Elisa; Stanger, Ben Z.; Sherr, Charles J.; Lowe, Scott W.
Source:
Cell 2020 v.181 no.2 pp. 424-441.e21
ISSN:
0092-8674
Subject:
endothelial cells,   etc   ; CD8-positive T-lymphocytes; adenocarcinoma; animal models; cytotoxicity; drug therapy; drugs; immunosuppression; immunotherapy; mice; mutants; pancreatic neoplasms; phenotype;     Show all 13 Subjects
Abstract:
... KRAS mutant pancreatic ductal adenocarcinoma (PDAC) is characterized by a desmoplastic response that promotes hypovascularity, immunosuppression, and resistance to chemo- and immunotherapies. We show that a combination of MEK and CDK4/6 inhibitors that target KRAS-directed oncogenic signaling can suppress PDAC proliferation through induction of retinoblastoma (RB) protein-mediated senescence. In p ...
DOI:
10.1016/j.cell.2020.03.008

15. A fluorinated low-molecular-weight PEI/HIF-1α shRNA polyplex system for hemangioma therapy

Author:
Guo, Xiaoshuang; Yuan, Zihan; Xu, Yang; Wei, Minyan; Fang, Zhiwei; Yuan, Wei-En
Source:
Biomaterials science 2020 v.8 no.8 pp. 2129-2142
ISSN:
2047-4849
Subject:
endothelial cells,   etc   ; RNA interference; anhydrides; blood serum; cations; cell proliferation; cytotoxicity; hemangioma; hypoxia-inducible factor 1; therapeutics; transfection;     Show all 11 Subjects
Abstract:
... Hemangioma, one of the most common angiogenic diseases in infants and children, is characterized by the abnormal and aggressive proliferation of vascular endothelial cells. Advanced therapeutic strategies like RNA interference can inhibit the expression of target proteins at the translational level, but they are rarely used in hemangioma treatment owing to the lack of safe carriers. In this study, ...
DOI:
10.1039/d0bm00171f

16. Macrophage-derived netrin-1 is critical for neuroangiogenesis in endometriosis

Author:
Guo, Xinyue; Ding, Shaojie; Li, Tiantian; Wang, Jianzhang; Yu, Qin; Zhu, Libo; Xu, Xinxin; Zou, Gen; Peng, Yangying; Zhang, Xinmei
Source:
International journal of biological macromolecules 2020 v.148 pp. 226-237
ISSN:
0141-8130
Subject:
endothelial cells,   etc   ; angiogenesis; ganglia; macrophages; mitogen-activated protein kinase; nerve tissue; neurites; neutralizing antibodies; phosphorylation; rats; receptors;     Show all 11 Subjects
Abstract:
... Netrin-1 is an extracellular guidance cue of neuronal navigation, mediated through interaction with its main receptors, and is known to be crucial in the development of multiple chronic inflammatory diseases. However, the expression pattern and mechanism of netrin-1 in endometriosis are currently undefined. Here we report that netrin-1 expression peaked in peritoneal macrophages found in endometri ...
DOI:
10.1016/j.ijbiomac.2020.01.130

17. PM2.5 induces autophagy and apoptosis through endoplasmic reticulum stress in human endothelial cells

Author:
Wang, Yan; Tang, Meng
Source:
The Science of the total environment 2020 v.710 pp. 136397
ISSN:
0048-9697
Subject:
endothelial cells,   etc   ; apoptosis; autophagy; cardiovascular system; cell viability; endoplasmic reticulum; endoplasmic reticulum stress; homeostasis; humans; lysosomes; particulates; protective effect;     Show all 12 Subjects
Abstract:
... Endothelial cells integrally form a crucial interface that maintains homeostasis of the cardiovascular system. As a vulnerable target of PM2.5, the underlying mechanisms of endothelial cell damage have yet to be fully elucidated. In the current study, two types of cell death, including autophagy and apoptosis, and an important organelle of the endoplasmic reticulum (ER) were focalized following PM ...
DOI:
10.1016/j.scitotenv.2019.136397

18. Repair strategies for injured peripheral nerve: Review

Author:
Raza, Chand; Riaz, Hasib Aamir; Anjum, Rabia; Shakeel, Noor ul Ain
Source:
Life sciences 2020 v.243 pp. 117308
ISSN:
0024-3205
Subject:
endothelial cells,   etc   ; axons; cell growth; engineering; extracellular matrix; nerve regeneration; nerve tissue; patients; peripheral nerves; polymers; stumps; surgeons; tissue transplantation;     Show all 13 Subjects
Abstract:
... Compromised functional regains in about half of the patients following surgical nerve repair pose a serious socioeconomic burden to the society. Although surgical strategies such as end-to-end neurorrhaphy, nerve grafting and nerve transfer are widely applied in distal injuries leading to optimal recovery; however in proximal nerve defects functional outcomes remain unsatisfactory. Biomedical engi ...
DOI:
10.1016/j.lfs.2020.117308

19. An integrated microfluidic device for studying controllable gas embolism induced cellular responses

Author:
Ma, Peng; Wang, Shanshan; Guan, Ruixue; Hu, Liang; Wang, Xixian; Ge, Anle; Zhu, Jinchi; Du, Wei; Liu, Bi-Feng
Source:
Talanta 2020 v.208 pp. 120484
ISSN:
0039-9140
Subject:
endothelial cells,   etc   ; calcium; calcium signaling; cell viability; drugs; embolism; gap junctions; ischemia; microbubbles; nucleotides; octanol; permeability; suramin;     Show all 13 Subjects
Abstract:
... Gas embolism is the abnormal emergence of bubble in the vascular system, which can induce local ischemic symptoms. For studying the mechanism underlying gas embolism and revealing local ischemic diseases information, novel technique for analyzing cells response to bubble contact with high controllability is highly desired. In this paper, we present an integrated microfluidic device for the precise ...
DOI:
10.1016/j.talanta.2019.120484

20. Thiol-Methylsulfone-Based Hydrogels for 3D Cell Encapsulation

Author:
Paez, Julieta I.; Farrukh, Aleeza; Valbuena-Mendoza, Rocío; Włodarczyk-Biegun, Małgorzata K.; del Campo, Aránzazu
Source:
ACS applied materials & interfaces 2020 v.12 no.7 pp. 8062-8072
ISSN:
1944-8252
Subject:
human umbilical vein endothelial cells,   etc   ; cell culture; chemical bonding; crosslinking; encapsulation; fibroblasts; hydrogels; ligands; mixing; models; pH; peptides; reaction kinetics;     Show all 13 Subjects
Abstract:
... Thiol-maleimide and thiol-vinylsulfone cross-linked hydrogels are widely used systems in 3D culture models, in spite of presenting uncomfortable reaction kinetics for cell encapsulation: too fast (seconds for thiol-maleimide) or too slow (minutes-hours for thiol-vinylsulfone). Here, we introduce the thiol-methylsulfone reaction as alternative cross-linking chemistry for cell encapsulation, particu ...
DOI:
10.1021/acsami.0c00709