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epicotyls, etc ; Mimosa pudica; fish; flow cytometry; fluorescence; heterochromatin; hybridization; hypocotyls; karyotyping; nuclear genome; silver; Show all 11 Subjects
Abstract:
... Chromosomal details and linear differentiation, including heterochromatin distribution, silver staining based characterization of NOR, delineation of chromomycin (CMA₃) and 4′,6-diamidino-2-phenylindole (DAPI) bands and FISH based localization of 45 S and 5 S rDNA sites are investigated for the first time for Mimosa pudica. All the three populations examined showed 2n = 4x = 52 with karyotype cons ...
... The use of exogenous compounds such as ‘gasotransmitter’ molecules is a well-established agronomic strategy to improve the crop tolerance to environmental stresses. In this current work, when Cr (200 µM) was combined with the nitric oxide (NO) generator sodium nitroprusside SNP, 500 µM) there was a suppression of metal-induced alterations in embryo growth. Exogenous NO produced by SNP reduced the ...
Oussama Kharbech; Lamia Sakouhi; Yethreb Mahjoubi; Marouane Ben Massoud; Ahmed Debez; Ons Talbi Zribi; Wahbi Djebali; Abdelilah Chaoui; Luis Alejandro Jose Mur
... The current research aimed to assess the protective role of nitric oxide (NO) against chromium (Cr) toxicity in maize seedlings. Chromium (200 µM) lowered osmotic potential in epicotyls and mostly in radicles (by 38% and 63%, respectively) as compared to the control. Sodium nitroprusside (SNP, NO donor) restored seedling biomass (+90% for both organs) and water potential, whereas application of Nω ...
epicotyls, etc ; calcium; drought tolerance; ethylene; hypocotyls; models; peas; proteome; proteomics; rehydration; research; stress response; Show all 12 Subjects
Abstract:
... The model of loss and re-establishment of desiccation tolerance (DT) in germinated seeds has been well developed to explore the mechanisms associated with DT, but little attention has been paid to the tissue variation in this model. Herein, we investigated DT in different embryo axis tissues of germinated pea seeds and its re-establishment by poly(ethylene glycol) (PEG) treatment and then employed ...
... In the current work, attempts were made to investigate culture of epicotyls segments with three different cuts (i.e. transverse cut, oblique cut, longitudinal cut), hypocotyls and cotyledon segments derived from in vitro seedlings of pectinifera rootstock. Effect of several factors i.e. culture medium, explants types on regeneration response were examined based on parameters, percentage of explant ...
epicotyls, etc ; cell walls; chemical bonding; chromatography; cutin; enzymes; epidermis (plant); oligosaccharides; pH; peas; plant physiology; polyesters; tomatoes; xyloglucans; Show all 14 Subjects
Abstract:
... The shoot epidermal cell wall in land-plants is associated with a polyester, cutin, which controls water loss and possibly organ expansion. Covalent bonds between cutin and its neighbouring cell-wall polysaccharides have long been proposed. However, the lack of biochemical evidence makes cutin–polysaccharide linkages largely conjectural. Here we optimised a portfolio of radiochemical assays to loo ...
... Citrus canker caused by Xanthomonas citri subsp. citri is one of the most devastating citrus diseases worldwide. Generating disease-resistant citrus varieties is considered one of the most efficient and environmentally friendly measures for controlling canker. X. citri subsp. citri causes canker symptoms by inducing the expression of canker susceptibility gene LOB1 via PthA4, a transcription activ ...
epicotyls, etc ; Enterococcus faecalis; Gram-positive bacteria; Staphylococcus aureus; agriculture; antibacterial properties; antibiotic resistance; antioxidant activity; antioxidants; artichokes; basil; gel chromatography; hydrophobicity; lignin; nuclear magnetic resonance spectroscopy; pepper; seed germination; Show all 17 Subjects
Abstract:
... BACKGROUND: Bioactive components isolated from composted agricultural biomasses have been receiving progressive attention, because they may improve the antibiotic susceptibility of drug resistant bacterial strains. Here, three different humic substances (HS) were isolated from composted artichoke (HS-CYN) and pepper (HS-PEP) wastes, and from coffee grounds (HS-COF), and characterized by infrared s ...
... Using a wide range of modern biotechnologies and genetic techniques to study plant germplasm accessions held by VIR makes it possible to procure valuable results, required for the development of new high-yielding cultivars adapted to adverse environmental conditions and possessing specified technological properties, particularly to identify and mark new genes and alleles useful for plant breeding. ...
... Industrial hemp (Cannabis sativa L., family Cannabaceae) is a multi-purpose crop, used in the production of food, nutraceuticals, cosmetics and medicines. Therefore, development of new varieties with specific chemical profiles is necessary. In vitro culture methods could be complementary to conventional breeding and a useful tool for large-scale propagation. Strong apical dominance is considered a ...
epicotyls, etc ; Aeschynomene; Dalbergia; Machaerium; Neotropics; cotyledons; hypocotyls; polyphyly; seedlings; Brazil; Show all 10 Subjects
Abstract:
... Although seedling morphology studies have contributed to the systematics of Leguminosae at different taxonomic levels, seedlings are poorly known in Aeschynomene, a pantropical genus with approximately 180 species. Aeschynomene traditionally comprises two sections in the Neotropics, A. sect. Aeschynomene and A. sect. Ochopodium. However, molecular phylogenies have indicated that the genus is proba ...
epicotyls, etc ; Escherichia coli; Gram-negative bacteria; Klebsiella pneumoniae; Pseudomonas aeruginosa; antioxidant activity; antioxidants; artichokes; basil; dietary supplements; humans; hydrophobicity; infrared spectroscopy; lignin; minimum inhibitory concentration; nuclear magnetic resonance spectroscopy; pepper; phytotoxicity; pyrolysis gas chromatography; seed germination; waste management; water solubility; Show all 22 Subjects
Abstract:
... The acknowledgement of bioactive functions of compost teas promotes the research on characteristics and potential application of these heterogeneous water-soluble extracts from recycled biomasses. In this work, compost teas were isolated from on-farm composts made with agro-industrial residues of artichoke, pepper and coffee husks with the aim to evaluate the structural-activity relationship of di ...
epicotyls, etc ; anisotropy; hypocotyls; microgravity; microtubules; Show all 5 Subjects
Abstract:
... The body shape of plants varied in proportion to the logarithm of the magnitude of gravity in the range from microgravity to hypergravity to resist the gravitational force. Here we discuss the roles of cortical microtubule and 65 kDa microtubule-associated protein-1 (MAP65-1) in gravity-induced modification of growth anisotropy. Microgravity stimulated elongation growth and suppressed lateral expa ...
... Relationships between changes in the levels of intracellular cAMP and H₂O₂ were studied in various growth zones of pea (Pisumsativum L.) seedling roots upon infection with Rhizobium leguminosarum bv. viceae (Rlv), Pseudomonas syringae pv. pisi (Psp), or Clavibacter michiganensis ssp. sepedonicus (Cms). Previous studies have shown that these root zones are differentially sensitive to Rlv. In the pr ...
... Rodents regularly rely on emerged epicotyls to locate and remove cotyledons still containing valuable nutrients. However, the extent to which acorn characteristics influence tolerance to post‐germination predation has received little attention. Here, we investigated the impact of cotyledon removal following epicotyl emergence on seedling performance and survival of seven oak (Quercus) species. We ...
... Rhamnogalacturonan I (RG-I), one of the pectic components of the plant cell wall, is composed of a backbone of repeating disaccharide units of rhamnose and galacturonic acid, and side chains, such as galactans, arabinans, and arabinogalactans. The activity of RG-I galactosyltransferase, which transfers galactosyl residues to rhamnosyl residues in the RG-I backbone, has not been detected until now. ...
epicotyls, etc ; 1-aminocyclopropane-1-carboxylate synthase; Momordica charantia; biosynthesis; ethephon; ethylene; ethylene production; female flowers; flowering; genes; gibberellic acid; high performance liquid chromatography; indole acetic acid; plant height; root growth; signal transduction; tandem mass spectrometry; transcription (genetics); Show all 18 Subjects
Abstract:
... Bitter gourd (Momordica charantia L.) is sensitive to phytohormone treatment for organ development and growth. In light of this, we performed this study to evaluate the influence of ethephon at physiological and transcriptional levels. High performance liquid chromatography – tandem mass spectrometry (HPLC-MS/MS) has demonstrated that ethylene biosynthesis was enhanced by increased ACC synthase (A ...
epicotyls, etc ; Pisum sativum; etiolation; gravitropism; mutants; peas; seedlings; Alaska; Show all 8 Subjects
Abstract:
... Negative gravitropic bending and its possible mediator in etiolated Alaska pea seedlings were intensively studied in comparison with seedlings of an agravitropic mutant, ageotropum. When 3.5-day-old etiolated Alaska seedlings were horizontally placed, the growth suppression at the upper side of the epicotyls began 10 min after the onset of the gravitropic stimulation, whereas the growth accelerati ...
... This manuscript reports the production of specific polyclonal antibodies for PsPIN1, a putative auxin efflux carrier in Alaska pea (Pisum sativum L.) plants, and the cellular immunolocalization of PsPIN1. When pea seeds were set with the seed axis horizontal to the upper surface of a rockwool block, and allowed to germinate and grow for 3 days in the dark, the epicotyl grew upward. On the other ha ...
... The effects of distinct UV-A and UV-B radiations were studied on etiolated pea (Pisum sativum L.) epicotyls. Emission spectra of the native protochlorophyll and protochlorophyllide forms were measured when epicotyls were excited with 360 or 300 nm light. The UV-A (360 nm) excited mainly the non-enzyme-bound monomers of protochlorophyll and protochlorophyllide and the UV-B (300 nm) excited preferen ...