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1. Degas-Driven Deterministic Lateral Displacement in Poly(dimethylsiloxane) Microfluidic Devices

Author:
Tottori, Naotomo; Nisisako, Takasi
Source:
Analytical chemistry 2019 v.91 no.4 pp. 3093-3100
ISSN:
1520-6882
Subject:
erythrocytes,   etc   ; fluorescence; leukocytes; polymers;     Show all 4 Subjects
Abstract:
... In this work, degas-driven microfluidic deterministic lateral displacement devices were fabricated from poly(dimethylsiloxane). Two device configurations were considered: one with a single input for the enrichment of particles and the other one with sheath inputs for the separation of particles based on their sizes. Using the single-input device, the characteristics of the degas-driven fluid throu ...
DOI:
10.1021/acs.analchem.8b05587

2. Magnetic Manipulation of Blood Conductivity with Superparamagnetic Iron Oxide-Loaded Erythrocytes

Author:
Philips, Gavin R.; Gleich, Bernhard; Paredes-Juarez, Genaro A.; Antonelli, Antonella; Magnani, Mauro; Bulte, Jeff W. M.
Source:
ACS applied materials & interfaces 2019 v.11 no.12 pp. 11194-11201
ISSN:
1944-8252
Subject:
erythrocytes,   etc   ; electrodes; electrophysiology; iron; magnetic fields; prototypes;     Show all 6 Subjects
Abstract:
... The active and passive electrophysiological properties of blood and tissue have been utilized in a vast array of clinical techniques to noninvasively characterize anatomy and physiology and to diagnose a wide variety of pathologies. However, the accuracy and spatial resolution of such techniques are limited by several factors, including an ill-posed inverse problem, which determines biological par ...
DOI:
10.1021/acsami.9b00394

3. Thermal sensitivity and haemolysis of erythrocytes with membranopathy

Author:
Ivanov, I.T.; Chakaarov, I.; Chakaarova, P.
Source:
Journal of thermal biology 2019 v.81 pp. 98-102
ISSN:
0306-4565
Subject:
erythrocyte membrane; erythrocytes,   etc   ; denaturation; heat; hemolysis; hemolytic anemia; patients; permeability; plasma membrane; spectrin; temperature;     Show all 11 Subjects
Abstract:
... Measuring the impedance of heated suspensions of erythrocytes and erythrocyte ghost membranes, two thermally-induced alterations are registered in the plasma membrane at TA (denaturation of spectrin with inducing temperature at 49,5 °C) and TG (hyperthermic activation of basal ion permeability with inducing temperature at 60.7 °C). In this study erythrocytes from 9 healthy patients and 15 patients ...
DOI:
10.1016/j.jtherbio.2019.02.019

4. Interactions of mannose binding-lectin with red blood cells by employing cationic quantum dots

Author:
Lima, Carinna N.; Cabral Filho, Paulo E.; Santos, Beate S.; Moura, Patrícia; Fontes, Adriana
Source:
International journal of biological macromolecules 2019 v.125 pp. 1168-1174
ISSN:
0141-8130
Subject:
erythrocyte membrane; erythrocytes,   etc   ; Candida albicans; antibodies; electrical charges; electrostatic interactions; fluorescence; lectins; mannose; quantum dots; rheology;     Show all 11 Subjects
Abstract:
... Mannose-binding lectin (MBL) plays important roles by interacting with specific molecular patterns on cell surfaces, triggering first-line host defense. We investigated the MBL interaction with healthy red blood cell membranes as well as its effects on the membrane rheology. We explored electrostatic interactions between cationic quantum dots (QDs) and negatively charged red blood cell surfaces to ...
DOI:
10.1016/j.ijbiomac.2018.12.098

5. PEG Bottle Brush Copolymers as Antimicrobial Mimics: Role of Entropic Templating in Membrane Lysis

Author:
Garle, Amit L.; Budhlall, Bridgette M.
Source:
Langmuir 2019 v.35 no.9 pp. 3372-3382
ISSN:
1520-5827
Subject:
erythrocyte membrane; erythrocytes,   etc   ; Escherichia coli; Gram-negative bacteria; composite polymers; dyes; encapsulation; ethanolamine; moieties; phospholipids; zwitterions;     Show all 11 Subjects
Abstract:
... Novel polymers containing quaternary functional groups, with and without (control copolymer) PEG side chains, were synthesized and characterized for their ability to lyse the phospholipid membranes of liposome vesicles. Calcein loaded unilamellar vesicles composed of 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) were used to mimic red-blood cell membranes, and a 80:20 (mol/mol) mixture of 1 ...
DOI:
10.1021/acs.langmuir.8b00756

6. Continuous erythrocyte removal and leukocyte separation from whole blood based on viscoelastic cell focusing and the margination phenomenon

Author:
Nam, Jeonghun; Yoon, Jung; Kim, Jeeyong; Jang, Woong Sik; Lim, Chae Seung
Source:
Journal of chromatography 2019 v.1595 pp. 230-239
ISSN:
0021-9673
Subject:
erythrocytes,   etc   ; blood vessels; centrifugation; chromatography; fluorescence; hematocrit; leukocytes; polymers; viscoelasticity;     Show all 9 Subjects
Abstract:
... The removal of erythrocytes from whole blood is an essential step during sample preparations intended for biomedical analyses and clinical diagnoses. To address the limitations of present methods, such as centrifugation and chemical lysis, we propose a novel microfluidic device for erythrocyte removal with high-efficiency and leukocyte separation from bulk flows of highly concentrated erythrocytes ...
DOI:
10.1016/j.chroma.2019.02.019

7. Biophysical measurement of red blood cells by laboratory on print circuit board chip

Author:
Zhao, Ying; Zhang, Wengeng
Source:
Electrophoresis 2019 v.40 no.8 pp. 1140-1143
ISSN:
0173-0835
Subject:
erythrocytes,   etc   ; adults; cost effectiveness; electrochemistry; electrodes; electrolytes; electrophoresis; humans;     Show all 8 Subjects
Abstract:
... Microfluidic impedance pulse sensor has emerged as an easily handled and low‐cost platform in the electrical analysis of biological cells. In the conventional method, impedance sensor demanded expensive patterning metal electrodes on the substrate, which are directly in touch with electrolytes in order to measure the microfluidic channel impedance change. In this article, a cost‐effective microflu ...
DOI:
10.1002/elps.201800123

8. Laser interference‐based technique for dynamic measurement of single cell deformation manipulated by optical tweezers

Author:
Liu, Jiaqi; Zhang, Fan; Zhu, Lianqing; Qu, Xinghua; Chu, Daping
Source:
Electrophoresis 2019 v.40 no.8 pp. 1160-1165
ISSN:
0173-0835
Subject:
erythrocytes,   etc   ; cameras; deformation; electrophoresis; human cell lines; optical traps; semiconductors;     Show all 7 Subjects
Abstract:
... A laser interference‐based method was proposed to measure the deformation response of cell manipulated by optical tweezers. This method was implemented experimentally by integrating a laser illuminating system and optical tweezers with an inverted microscope. Interference fringes generated by the transmitted and reflected lights were recorded by a complementary metal oxide semiconductor camera. Fr ...
DOI:
10.1002/elps.201800336

9. Impedance‐based viscoelastic flow cytometry

Author:
Serhatlioglu, Murat; Asghari, Mohammad; Tahsin Guler, Mustafa; Elbuken, Caglar
Source:
Electrophoresis 2019 v.40 no.6 pp. 906-913
ISSN:
0173-0835
Subject:
erythrocytes,   etc   ; electrophoresis; flow cytometry; humans; polyethylene glycol; polystyrenes; viscoelasticity;     Show all 7 Subjects
Abstract:
... Elastic nature of the viscoelastic fluids induces lateral migration of particles into a single streamline and can be used by microfluidic based flow cytometry devices. In this study, we investigated focusing efficiency of polyethylene oxide based viscoelastic solutions at varying ionic concentration to demonstrate their use in impedimetric particle characterization systems. Rheological properties ...
DOI:
10.1002/elps.201800365

10. Proton-controlled synthesis of red-emitting carbon dots and application for hematin detection in human erythrocytes

Author:
Ju, Yan Jun; Li, Na; Liu, Shi Gang; Liang, Jia Yu; Gao, Xin; Fan, Yu Zhu; Luo, Hong Qun; Li, Nian Bing
Source:
Analytical and bioanalytical chemistry 2019 v.411 no.6 pp. 1159-1167
ISSN:
1618-2642
Subject:
erythrocytes,   etc   ; carbon quantum dots; fluorescent dyes; humans; optical properties; pH;     Show all 6 Subjects
Abstract:
... The Red-emitting nitrogen-doped carbon dots (N-CDs) are synthesized using o-phenylenediamine by a one-step method, and can serve as a fluorescent probe for “turn off” detection of hematin in human red cells. The red-emitting N-CDs can be obtained only in acidic conditions and the emission of the red-emitting N-CDs is pH-dependent, indicating proton-controlled synthesis and emission. The red-emitti ...
DOI:
10.1007/s00216-018-1547-z

11. Influence of Type of Use, Age and Gender on Haematological and Biochemical Blood Parameters of Małopolski Horses

Author:
Czech, Anna; Kiesz, Martyna; Kiesz, Adrian; Próchniak, Tomasz; Różański, Paweł; Klimiuk, Kamila
Source:
Annals of animal science 2019 v.19 no.1 pp. 85-96
ISSN:
2300-8733
Subject:
erythrocytes,   etc   ; bone formation; calcium; exercise; mares; neutrophils; phosphorus; sports; stallions;     Show all 9 Subjects
Abstract:
... The purpose of the study was to determine whether the type of use, age and gender of Małopolski horses affect the level of selected haematological and biochemical parameters of their blood and whether there is an interaction between these factors. The research was carried out on 30 horses of the Małopolski breed, which were divided according to gender (18 mares and 12 stallions), age (2–6 years, 7 ...
DOI:
10.2478/aoas-2018-0031

12. Highly sensitive and wide-range nanoplasmonic detection of fibrinogen using erythrocyte membrane-blanketed nanoparticles

Author:
Jo, Seongjae; Kim, Insu; Lee, Wonseok; Kim, Minwoo; Park, Joohyung; Lee, Gyudo; Yoon, Dae Sung; Park, Jinsung
Source:
Biosensors & bioelectronics 2019 v.135 pp. 216-223
ISSN:
0956-5663
Subject:
erythrocyte membrane; erythrocytes,   etc   ; angiogenesis; biomarkers; biosensors; blood coagulation; cardiovascular diseases; fibrinogen; fibroblasts; glycoproteins; hemophilia; nanoparticles; plasma membrane; surface plasmon resonance;     Show all 14 Subjects
Abstract:
... Fibrinogen, which is a glycoprotein that circulates in the blood, plays various important biological roles, e.g., in blood coagulation, fibroblast proliferation, angiogenesis, and wound healing. Abnormal levels of fibrinogen in plasma have been identified as a key biomarker of a variety of disorders from cardiovascular diseases to hemophilia. Therefore, the development of a quantitative assay for ...
DOI:
10.1016/j.bios.2019.04.030

13. Electrochemical impedance spectroscopy of blood. Part 3: a study of the correlation between blood conductivity and sedimentation to shorten the erythrocyte sedimentation rate test

Author:
Zhbanov, A.; Yang, S.
Source:
Analytical methods 2018 v.10 no.2 pp. 180-189
ISSN:
1759-9679
Subject:
erythrocyte sedimentation rate; erythrocytes,   etc   ; cameras; dielectric spectroscopy; hematocrit; mathematical models;     Show all 6 Subjects
Abstract:
... The study of erythrocyte aggregation and erythrocyte sedimentation rate (ESR) is very important both for basic research and medical applications. The duration of the Westergren ESR test is one hour which seems excessive. The hematocrit and conductivity of blood demonstrate high correlation. This makes it possible to evaluate the erythrocyte aggregation kinetics and ESR by measuring changes in the ...
DOI:
10.1039/c7ay01696d

14. Intermolecular ionic interactions serve as a possible switch for stem release in the staphylococcal bi-component toxin for β-barrel pore assembly

Author:
Takeda, Kein; Tanaka, Yoshikazu; Abe, Naoki; Kaneko, Jun
Source:
Toxicon 2018 v.155 pp. 43-48
ISSN:
0041-0101
Subject:
erythrocytes,   etc   ; electrostatic interactions; hemolysis; mutation;     Show all 4 Subjects
Abstract:
... The β-strand stem release system of staphylococcal β-barrel pore-forming toxin γ-hemolysin was investigated. Mutations at K15 and R16 in the cap domain of Hlg2 decreased hemolytic activity more markedly than their effect on erythrocyte binding. In addition, D122N mutation of LukF prestem lost the activity with Hlg2 R16A, indicating that electrostatic interactions between residues in the Hlg2 cap a ...
DOI:
10.1016/j.toxicon.2018.10.002

15. Hexachloronaphthalene as a hemostasis disturbing factor in female Wistar rats – A pilot study

Author:
Kilanowicz, Anna; Markowicz-Piasecka, Magdalena; Klimczak, Michał; Stragierowicz, Joanna; Sikora, Joanna
Source:
Chemosphere 2019 v.228 pp. 577-585
ISSN:
0045-6535
Subject:
erythrocyte membrane; erythrocytes,   etc   ; adverse effects; blood platelet count; chlorinated naphthalenes; coagulation; females; fibrinogen; fibrinolysis; hematology; hemostasis; laboratory animals; persistent organic pollutants; polychlorinated dibenzodioxins; rats; thromboplastin; toxic substances; toxicity;     Show all 18 Subjects
Abstract:
... Although Persistent Organic Pollutants (POPs) are some of the most dangerous environmental toxicants, data on their impact on hemostasis are virtually limited. 1,2,3,5,6,7-hexachloronaphthalene (PCN67) seems to be one of the most toxic congeners of polychlorinated naphthalenes (PCNs), which have recently been listed as POPs. The toxic effects of PCNs are similar to other chlorinated aromatics, e.g ...
DOI:
10.1016/j.chemosphere.2019.04.147

16. More than just the numbers—contrasting response of snake erythrocytes to thermal acclimation

Author:
Bury, Stanisław; Bury, Agata; Sadowska, Edyta T.; Cichoń, Mariusz; Bauchinger, Ulf
Source:
Die Naturwissenschaften 2019 v.106 no.5-6 pp. 24
ISSN:
0028-1042
Subject:
erythrocyte count; erythrocytes,   etc   ; Natrix natrix; acclimation; ambient temperature; blood flow; blood viscosity; cold; ectothermy; endothermy; energy expenditure; hematocrit; hemoglobin; metabolism; models; oxygen; oxygen consumption; snakes;     Show all 18 Subjects
Abstract:
... Acclimation to lower temperatures decreases energy expenditure in ectotherms but increases oxygen consumption in most endotherms, when dropped below thermoneutrality. Such differences should be met by adjustments in oxygen transport through blood. Changes in hematological variables in correspondence to that in metabolic rates are, however, not fully understood, particularly in non-avian reptiles. ...
DOI:
10.1007/s00114-019-1617-x

17. Decreased erythrocyte CD44 and CD58 expression link e-waste Pb toxicity to changes in erythrocyte immunity in preschool children

Author:
Huo, Xia; Dai, Yifeng; Yang, Tian; Zhang, Yu; Li, Minghui; Xu, Xijin
Source:
The Science of the total environment 2019 v.664 pp. 690-697
ISSN:
0048-9697
Subject:
erythrocyte count; erythrocytes,   etc   ; biomarkers; electronic wastes; immunity; interferon-gamma; interleukin-12; interleukin-1beta; interleukin-2; lead; leukocyte count; long term effects; preschool children; regression analysis; secretion; toxicity;     Show all 16 Subjects
Abstract:
... Lead (Pb) toxicity damages blood cells and disturbs the immune micro-environment. When Pb enters the circulatory system, >95% of Pb accumulates in erythrocytes. We therefore conducted this study to explore the long-term effect of Pb exposure on expression of erythrocyte adhesion molecules (CD44 and CD58) and related downstream cytokine concentrations. We enrolled a total of 267 preschool children, ...
DOI:
10.1016/j.scitotenv.2019.02.040

18. Erythrocyte membrane camouflaged graphene oxide for tumor-targeted photothermal-chemotherapy

Author:
Li, Jian; Huang, Xueyuan; Huang, Rong; Jiang, Jing; Wang, Yanjie; Zhang, Junhua; Jiang, Haiye; Xiang, Xinying; Chen, Wansong; Nie, Xinmin; Gui, Rong
Source:
Carbon 2019 v.146 pp. 660-670
ISSN:
0008-6223
Subject:
erythrocyte membrane; erythrocytes,   etc   ; biocompatibility; blood circulation; doxorubicin; drug therapy; folic acid; graphene oxide; in vivo studies; nanoparticles; neoplasm cells; neoplasms; photosensitizing agents; reticuloendothelial system;     Show all 14 Subjects
Abstract:
... Nanodrug carrier-based cancer therapy has been actively developed in the past decades. The main challenges faced by nanodrug carriers include poor drug loading capacity, rapid clearance from blood circulation, and low antitumor efficiency. In this work, a new type of antineoplastic agents, F-RGID, were developed using a two-dimensional graphene oxide (GO) with an incorporated photosensitizer (Indo ...
DOI:
10.1016/j.carbon.2019.02.056

19. An in vitro study of the protective effect of caffeic acid on human erythrocytes

Author:
Colina, José R.; Suwalsky, Mario; Manrique-Moreno, Marcela; Petit, Karla; Aguilar, Luis F.; Jemiola-Rzeminska, Malgorzata; Strzalka, Kazimierz
Source:
Archives of biochemistry and biophysics 2019 v.662 pp. 75-82
ISSN:
0003-9861
Subject:
erythrocyte membrane; erythrocytes,   etc   ; X-ray diffraction; caffeic acid; differential scanning calorimetry; fluorescence emission spectroscopy; humans; in vitro studies; molecular models; phospholipids; protective effect; scanning electron microscopy;     Show all 12 Subjects
Abstract:
... The interaction and protective effect of caffeic acid (CA) on human erythrocytes (RBC) and molecular models of its membrane were studied. The latter consisted of bilayers built up of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipid classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. X ...
DOI:
10.1016/j.abb.2018.12.006

20. The effect of dipotassium EDTA and lithium heparin on hematologic values of farmed brown trout Salmo trutta (L.) spawners

Author:
Ciepliński, Mateusz; Kasprzak, Mariusz; Grandtke, Monika; Steliga, Aleksandra; Kamiński, Piotr; Jerzak, Leszek
Source:
Aquaculture international 2019 v.27 no.1 pp. 79-87
ISSN:
0967-6120
Subject:
erythrocyte count; erythrocytes,   etc   ; EDTA (chelating agent); Salmo trutta; animal health; anticoagulants; environmental factors; fish; hematocrit; hematology; hemoglobin; hemolysis; heparin; leukocyte count; leukocytes; lithium; normal values; spawning;     Show all 18 Subjects
Abstract:
... Blood analysis is a very important and powerful diagnostic tool in animal health and welfare control. It is routinely performed in higher vertebrates, for which reference values are well established, but fish hematology still needs further research. Many intrinsic and environmental factors have profound impact on fish hematological values, making determination of reference values difficult. Additi ...
DOI:
10.1007/s10499-018-0308-5