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1. Quantification of hepatitis E virus in raw pork livers using droplet digital RT-PCR

Author:
Kairui Wang; Libing Liu; Jinfeng Wang; Xiaoxia Sun; Qingan Han; Cang Zhou; Xiangdong Xu; Jianchang Wang
Source:
Food microbiology 2023 v.109 pp. 104114
ISSN:
0740-0020
Subject:
etiological agents,   etc   ; Orthohepevirus A; RNA; biosafety; detection limit; droplets; food microbiology; genes; hepatitis E; livestock; phylogeny; pork; reverse transcription; viral load; viruses; China;     Show all 16 Subjects
Abstract:
... Hepatitis E virus (HEV) is the causative agent of hepatitis E. Some of the rise in hepatitis E infection in China may be linked to undercooked pork. In this study, we established a reverse transcription droplet digital PCR (RT-ddPCR) method to detect HEV in raw pork livers. The detection limit of the assay for HEV RNA was as low as 1.81 copies/μL. The suggested approach was validated on 14 samples ...
DOI:
10.1016/j.fm.2022.104114

2. Atypical porcine pestivirus—A widespread virus in the Swedish wild boar population

Author:
Hedvig Stenberg; Elena Leveringhaus; Anna Malmsten; Anne‐Marie Dalin; Alexander Postel; Maja Malmberg
Source:
Transboundary and emerging diseases 2022 v.69 no.4 pp. 2349-2360
ISSN:
1865-1674
Subject:
etiological agents,   etc   ; Pestivirus K; blood serum; seroprevalence; viruses; wild boars; Sweden;     Show all 7 Subjects
Abstract:
... The recently identified causative agent of congenital tremor in domestic piglets, atypical porcine pestivirus (APPV), was detected in serum from Swedish wild boar. A previous study from Sweden described APPV in domestic piglets suffering from congenital tremor, but the APPV situation in the wild boar population was unknown. In this study, 595 serum samples from wild boar originating from 13 counti ...
DOI:
10.1111/tbed.14251

3. First report of leaf anthracnose caused by Colletotrichum chrysophilum on açaí palm (Euterpe oleracea Mart.) in Brazil

Author:
Gleiciane Rodrigues dos Santos; Alessandra Jackeline Guedes de Moraes; Alice de Paula de Sousa Cavalcante; Gisele Barata da Silva
Source:
Journal of general plant pathology 2022 v.88 no.3 pp. 212-216
ISSN:
1345-2630
Subject:
etiological agents,   etc   ; Colletotrichum; Euterpe oleracea; anthracnose; leaves; pathogenicity; plant pathology; Brazil;     Show all 8 Subjects
Abstract:
... Açaí palm anthracnose caused by Colletotrichum chrysophilum is reported for the first time in Brazil. The etiological agent was identified by a combination of morphological and molecular approaches. Cultural morphological of the identified causal agent were consistent and resembled those described for C. chrysophilum. Pathogenicity was confirmed and Koch’s postulates fulfilled for a selected isola ...
DOI:
10.1007/s10327-022-01059-4

4. Cutaneous disseminated sporotrichosis in immunocompetent patient: Case report and literature review

Author:
Flávio Queiroz-Telles; Regielly Caroline Cognialli; Gabriel Lucca Salvador; Gabriela Araujo Moreira; Patricia Fernanda Herkert; Ferry Hagen
Source:
Medical mycology case reports 2022 v.36 pp. 31-34
ISSN:
2211-7539
Subject:
etiological agents,   etc   ; Sporothrix; case studies; farmers; itraconazole; mycology; patients; sporotrichosis; Brazil;     Show all 9 Subjects
Abstract:
... Sporotrichosis is a global occurring implantation (subcutaneous) mycosis, caused by Sporothrix species, usually affecting the skin and the lymphatic vessels, from where it can disseminate. Nowadays, the vast majority of sporotrichosis infections in Brazil are caused by zoonotic transmission of S. brasiliensis which is the main etiologic agent. We report a cutaneous disseminated case of this diseas ...
DOI:
10.1016/j.mmcr.2022.05.003

5. Non-agreement between 2 serologic techniques for detecting antibody to Brucella ovis in naturally infected sheep

Author:
José V. P. Barreto; Patrícia A. M. Oliveira; Simone F. N. Pertile; Maria C. R. Sbizera; Fabiola C. A. Rego; Gustavo R. Queiroz; Luiz F. C. Cunha Filho
Source:
Journal of veterinary diagnostic investigation 2022 v.34 no.1 pp. 164-166
ISSN:
1943-4936
Subject:
etiological agents,   etc   ; Brucella melitensis biovar Ovis; agar; antibodies; blood serum; brucellosis; gels;     Show all 7 Subjects
Abstract:
... Brucella ovis is the causative agent of ovine brucellosis, which is an important infectious disease in sheep farming worldwide and is responsible for economic losses because of its negative effect on the reproductive system of rams and ewes. Serologic tests are the main tools for detection of infection; however, these tests commonly yield a high frequency of false-negative results. We compared 2 s ...
DOI:
10.1177/10406387211053589

6. Phi 6 recovery from inoculated fingerpads based on elution buffer and methodology

Author:
Christopher A. Baker; Kristen E. Gibson
Source:
Journal of virological methods 2022 v.299 pp. 114307
ISSN:
0166-0934
Subject:
etiological agents,   etc   ; bacteriophages; beef extracts; disease transmission; gloves; humans; hygiene; juices; phosphates;     Show all 9 Subjects
Abstract:
... Phi 6 (Φ6) bacteriophage is a proposed surrogate to study pathogenic enveloped viruses including SARS-CoV-2—the causative agent of COVID-19—based on structural similarities, BSL-1 status, and ease of use. To determine the role of virus-contaminated hands in disease transmission, an enhanced understanding of buffer and method performance for Φ6 recovery needs to be determined. Four buffer types and ...
DOI:
10.1016/j.jviromet.2021.114307

7. gltA typing of Anaplasma strains related to A. platys: Taxonomical and one health implications

Author:
Rosanna Zobba; Eleonora Schianchi; Mourad Ben Said; Hanène Belkahia; Lilia Messadi; Roberta Piredda; Marco Pittau; Alberto Alberti
Source:
Ticks and tick-borne diseases 2022 v.13 no.1 pp. 101850
ISSN:
1877-959X
Subject:
etiological agents,   etc   ; Anaplasma phagocytophilum; dogs; genes; humans; phylogeny; public health; thrombocytopenia; China;     Show all 9 Subjects
Abstract:
... Species belonging to the genus Anaplasma (Rickettsiales) include bacteria of veterinary and public health importance. Beside the zoonotic Anaplasma phagocytophilum, A. platys, the etiological agent of canine cyclic thrombocytopenia, has been sporadically reported in clinically ill human patients. The ongoing emergence of novel strains related to this species in vertebrate hosts emphasises the need ...
DOI:
10.1016/j.ttbdis.2021.101850

8. The Paradox of the Mediterranean Diet in Pediatric Age during the COVID-19 Pandemic

Author:
Elvira Verduci; Giulia Fiore; Elisabetta Di Profio; Gian Vincenzo Zuccotti
Source:
Nutrients 2022 v.14 no.3 pp. -
ISSN:
2072-6643
Subject:
etiological agents,   etc   ; COVID-19 infection; Mediterranean diet; Severe acute respiratory syndrome coronavirus 2;     Show all 4 Subjects
Abstract:
... The outbreak of the COVID-19 pandemic, whose causative agent is Severe Acute Respiratory Syndrome Coronavirus 2, has caused a global crisis that has had a major impact on the health of the global population [...] ...
DOI:
10.3390/nu14030705

9. Prevotella oris: A lesser known etiological agent of pleural effusion

Author:
Lakshmi Shree Viswanath; Anitha Gunalan; Imola Jamir; Balamurugan S; Anbarasan S; Aswin K; Rakhi Biswas
Source:
Anaerobe 2022 v.78 pp. 102644
ISSN:
1075-9964
Subject:
etiological agents,   etc   ; Prevotella oris; X-radiation; anaerobes; chest; digestive tract; lungs; mouth; pain;     Show all 9 Subjects
Abstract:
... Prevotella are gram negative, obligate anaerobes and includes pigmented and non-pigmented species. They are typically regarded as commensals, especially of the gastrointestinal tract and oral cavity, although opportunistic endogenous infections are known to be caused by a small number of strains. We report a case of a 51-year-old man with right-sided chest pain. Chest X-ray showed mild haziness in ...
DOI:
10.1016/j.anaerobe.2022.102644

10. An investigation on bloom dynamics of Alexandrium catenella and A. pacificum and toxin accumulation in shellfish along the coast of Qinhuangdao, China

Author:
Wen-Jiao Tang; Zhuo-Ru Lin; Qing-Chun Zhang; Hui-Xia Geng; Hong-Xiao Sun; Xue-Xi Tang; Ren-Cheng Yu
Source:
Marine pollution bulletin 2022 v.183 pp. 114058
ISSN:
0025-326X
Subject:
etiological agents,   etc   ; Alexandrium catenella; coasts; marine pollution; phytoplankton; shellfish; toxicity; China; Yellow Sea;     Show all 9 Subjects
Abstract:
... The dinoflagellate genus Alexandrium comprises most of the toxic bloom-forming species producing paralytic shellfish toxins (PSTs) in the sea. Recently, repeated paralytic shellfish poisoning episodes have been recorded in Qinhuangdao located at the west coast of the Bohai Sea. To elucidate the relationship between toxic Alexandrium blooms and the poisoning episodes, a year-round investigation was ...
DOI:
10.1016/j.marpolbul.2022.114058

11. A rare case of ocular filariasis caused by Brugia malayi

Author:
Chitchanok Tantipat; Metta Wongkamchai; Kati Zaelai; Ameorn Amesombun; Sirichit Wongkamchai
Source:
Parasitology international 2022 v.90 pp. 102606
ISSN:
1383-5769
Subject:
etiological agents,   etc   ; Brugia malayi; conjunctiva; eyelids; filariasis; humans; migratory behavior; parasitology; women; Thailand;     Show all 10 Subjects
Abstract:
... Extra-lymphatic manifestation of filariasis is uncommon and may usually be clinically misdiagnosed. The extra lymphatic presentation of Brugia malayi may due to the site of entry of the infective larvae, and only a few cases have been proven for the causative species. We report here a 59-year-old woman presented with swollen right conjunctiva and complaint of migratory swelling at her eyelid, whic ...
DOI:
10.1016/j.parint.2022.102606

12. Biological characteristics of the Trypanosoma cruzi Arequipa strain make it a good model for Chagas disease drug discovery

Author:
Rubén Martín-Escolano; María José Rosales; Clotilde Marín
Source:
Acta tropica 2022 v.236 pp. 106679
ISSN:
0001-706X
Subject:
etiological agents,   etc   ; Chagas disease; Trypanosoma cruzi; benznidazole; genetic variation; models; parasitemia; parasites; therapeutics;     Show all 9 Subjects
Abstract:
... Trypanosoma cruzi, the causative agent of Chagas disease (CD), is a genuine parasite with tremendous genetic diversity and a complex life cycle. Scientists have studied this disease for more than 100 years, and CD drug discovery has been a mainstay due to the absence of an effective treatment. Technical advances in several areas have contributed to a better understanding of the complex biology and ...
DOI:
10.1016/j.actatropica.2022.106679

13. Penicillinase plasmid Australia type in Neisseria gonorrhoeae isolated in Poland

Author:
Szymon Walter de Walthoffen
Source:
Archives of microbiology 2022 v.204 no.2 pp. 130
ISSN:
0302-8933
Subject:
etiological agents,   etc   ; Neisseria gonorrhoeae; beta-lactamase; ceftriaxone; genes; plasmids; public health; Australia; Poland;     Show all 9 Subjects
Abstract:
... PURPOSE: Neisseria gonorrhoeae is an etiological agent of gonorrhea which remains a major public health problem the mechanisms that determine resistance to drugs of the beta-lactam class, which are recommended for the treatment of gonorrhea, are currently the most important problem in its treatment. Chromosomal mutations are responsible for resistance to ceftriaxone and cefepime. The possibility o ...
DOI:
10.1007/s00203-021-02623-w

14. Efficacy of flukicides on Fasciola gigantica, a food-borne zoonotic helminth affecting livestock in Bangladesh

Author:
Mohammad Manjurul Hasan; Babul Chandra Roy; Hiranmoy Biswas; Moizur Rahman; Anisuzzaman Anisuzzaman; Mohammad Zahangir Alam; Md. Hasanuzzaman Talukder
Source:
Parasitology 2022 v.149 no.10 pp. 1339-1348
ISSN:
1469-8161
Subject:
etiological agents,   etc   ; Fasciola gigantica; fascioliasis; flukes; histopathology; livestock; nitroxinil; oxyclozanide; parasitology; triclabendazole; Bangladesh;     Show all 11 Subjects
Abstract:
... Fasciola gigantica, the causative agent of tropical fasciolosis, is a food-borne zoonotic trematode that affects around 80% livestock of Bangladesh. Triclabendazole (TCBZ), nitroxynil (NTON) and oxyclozanide (OCZN) are frequently used against fascioliasis; however, the current status of potency of these flukicides was unknown. In this study, in vitro efficacy of TCBZ, NTON and OCZN at various conc ...
DOI:
10.1017/S0031182022000580

15. Establishment of different challenge models for Aeromonas salmonicida subsp. achromogenes in turbot and sole

Author:
Yolanda Torres-Corral; Oscar González-Barreiro; Ana Riaza; Ysabel Santos
Source:
Aquaculture 2022 v.555 pp. 738261
ISSN:
0044-8486
Subject:
etiological agents,   etc   ; Scophthalmus maximus; Solea senegalensis; aquaculture; furunculosis; host specificity; mortality; septicemia; turbot;     Show all 9 Subjects
Abstract:
... Aeromonas salmonicida subsp. achromogenes is one of the causative agents of “atypical furunculosis”, characterized by the presence of dermal ulcerations and external pathology with or without subsequent septicaemia. This study was aimed to identify possible routes of infection of A. salmonicida subsp. achromogenes in turbot (Scophthalmus maximus) and sole (Solea senegalensis). Thus, challenge test ...
DOI:
10.1016/j.aquaculture.2022.738261

16. Human‐mediated trophic mismatch between fire, plants and herbivores

Author:
Marcus A. Lashley; M. Colter Chitwood; Jacob L. Dykes; Christopher S. DePerno; Christopher E. Moorman
Source:
Ecography 2022 v.2022 no.3 pp. e06045
ISSN:
0906-7590
Subject:
etiological agents,   etc   ; climate; field experimentation; herbivores; humans; lactation; lightning; phenology; Southeastern United States;     Show all 9 Subjects
Abstract:
... Trophic mismatches are commonly reported across a wide array of taxa and can have important implications for species participating in the interaction. However, to date, examples of trophic mismatch have centrally focused on those induced by shifts in climate. Here we report on the potential for humans to induce trophic mismatch by shifting the phenology of fire. Globally, anthropogenic fire igniti ...
DOI:
10.1111/ecog.06045

17. Long-Read Sequencing and Hybrid Assembly for Genomic Analysis of Clinical Brucella melitensis Isolates

Author:
Hillary A. Craddock; Yair Motro; Bar Zilberman; Boris Khalfin; Svetlana Bardenstein; Jacob Moran-Gilad
Source:
Microorganisms 2022 v.10 no.3 pp. -
ISSN:
2076-2607
Subject:
etiological agents,   etc   ; Brucella melitensis; brucellosis; computer simulation; databases; genomics; nanopores; phylogeny; virulence; Israel;     Show all 10 Subjects
Abstract:
... Brucella melitensis is a key etiological agent of brucellosis and has been increasingly subject to characterization using sequencing methodologies. This study aimed to investigate and compare short-read, long-read, and hybrid assemblies of B. melitensis. Eighteen B. melitensis isolates from Southern Israel were sequenced using Illumina and the Oxford Nanopore (ONP) MinION, and hybrid assemblies we ...
DOI:
10.3390/microorganisms10030619

18. Ophidiomyces ophidiicola detection and infection: a global review on a potential threat to the world’s snake populations

Author:
Matteo R. Di Nicola; Luca Coppari; Tommaso Notomista; Daniele Marini
Source:
European journal of wildlife research 2022 v.68 no.5 pp. 64
ISSN:
1612-4642
Subject:
etiological agents,   etc   ; captive animals; fungi; monitoring; mortality; mycoses; predation; research; snakes; thermoregulation; wildlife;     Show all 11 Subjects
Abstract:
... Ophidiomyces ophidiicola (Oo) is one of the most relevant fungal pathogens for snakes. It is the etiological agent of ophidiomycosis, an emerging disease causing dysecdysis, skin abnormalities, crusting cutaneous lesions, and ulcerations. Despite this major tegumentary “tropism”, Oo infection can be systemic and it is capable of inducing visceral lesions. Moreover, ophidiomycosis may lead to abnor ...
DOI:
10.1007/s10344-022-01612-8

19. Taylorella asinigenitalis: raising awareness of its importance and presence in equine and asinine populations

Author:
Abel Dorrego; Consuelo Serres; Fatima Cruz‐Lopez
Source:
Veterinary record 2022 v.190 no.6 pp. e1602
ISSN:
0042-4900
Subject:
etiological agents,   etc   ; Taylorella asinigenitalis; Taylorella equigenitalis; asses; contagious equine metritis; horses; surveys; Spain;     Show all 8 Subjects
Abstract:
... Taylorella equigenitalis has long been recognised as a causative agent of contagious equine metritis, but practitioners may be less familiar with Taylorella asinigenitalis, which has been identified more recently. Here, Abel Dorrego, Consuelo Serres and Fatima Cruz‐Lopez of the Universidad Complutense de Madrid describe T asinigenitalis and report the findings of a survey they carried out in donke ...
DOI:
10.1002/vetr.1602

20. Bacterial Antagonistic Species of the Pathogenic Genus Legionella Isolated from Cooling Tower

Author:
Kiran Paranjape; Simon Lévesque; Sébastien P. Faucher
Source:
Microorganisms 2022 v.10 no.2 pp. -
ISSN:
2076-2607
Subject:
etiological agents,   etc   ; Legionella pneumophila; agar; antimicrobial properties; genes; genetic variation; pneumonia; probability; sequence analysis;     Show all 9 Subjects
Abstract:
... Legionella pneumophila is the causative agent of Legionnaires’ disease, a severe pneumonia. Cooling towers are a major source of large outbreaks of the disease. The growth of L. pneumophila in these habitats is influenced by the resident microbiota. Consequently, the aim of this study was to isolate and characterize bacterial species from cooling towers capable of inhibiting several strains of L. ...
DOI:
10.3390/microorganisms10020392