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1. Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates

Author:
Morais, Stephanie L.; Barros, Piedade; Santos, Marlene; Delerue-Matos, Cristina; Gomes, Andreia C.; Fátima Barroso, M.
Source:
Talanta 2021 v.222 pp. 121416
ISSN:
0039-9140
Subject:
fluorescein,   etc   ; Alexandrium minutum; DNA; DNA probes; databases; electrochemistry; electrodes; genes; gold; nucleic acid hybridization; toxicity; Atlantic Ocean;     Show all 12 Subjects
Abstract:
... This work addresses the development of a disposable electrochemical genosensor for the detection of the toxic dinoflagellate, Alexandrium minutum. Analyzing public databases, a specific 70 bp DNA probe, targeting A. minutum, was selected and designed. The genosensor methodology implied the immobilization of a A. minutum-specific DNA-capture probe onto screen-printed gold electrodes (SPGE). To impr ...
DOI:
10.1016/j.talanta.2020.121416

2. 3D-printed and fully portable fluorescent-based platform for sulfide determination in waters combining vapor generation extraction and digital images treatment

Author:
Leal, Vanderli Garcia; Batista, Alex Domingues; Petruci, João Flávio da Silveira
Source:
Talanta 2021 v.222 pp. 121558
ISSN:
0039-9140
Subject:
fluorescein,   etc   ; absorbance; acetates; chemical reactions; digital images; diodes; fluorescence; hydrogen sulfide; mercury; mobile telephones; monitoring; phosphoric acid; pumps; reflectance; river water; tap water; three-dimensional printing; toxicity; vapors; wastewater;     Show all 20 Subjects
Abstract:
... The determination of sulfide anion in a variety of waters (e.g. wastewaters and natural waters) even at low concentration (i.e. in the μM range) is essential due to its high toxicity, corrosivity and unpleasant smelling proprieties. Despite several methodologies are dedicated to aqueous sulfide determination, most of them need sampling/transport steps – which is no adequate to sulfide due to its r ...
DOI:
10.1016/j.talanta.2020.121558

3. Tricolor imaging of MMPs to investigate the promoting roles of inflammation on invasion and migration of tumor cells

Author:
Guo, Wenfei; Gao, Xiaonan; Zhan, Renhui; Zhao, Zengteng; Xu, Kehua; Tang, Bo
Source:
Talanta 2021 v.222 pp. 121525
ISSN:
0039-9140
Subject:
fluorescein,   etc   ; biomarkers; chemical bonding; confocal laser scanning microscopy; energy transfer; fluorescence; gelatinase A; gelatinase B; gold; image analysis; inflammation; isothiocyanates; lipopolysaccharides; matrilysin; migratory behavior; nanogold; neoplasm cells; peptides; selenium;     Show all 19 Subjects
Abstract:
... The expression levels of matrix metalloproteinases (MMPs) are closely related to the degree of inflammation which facilitates tumor cells' invasion and migration. A tricolor fluorescence nanoprobe based on high-fidelity gold-selenium (Au–Se) nanoplatform was designed and constructed for simultaneously imaging matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-7 (MMP-7) and matrix metallo ...
DOI:
10.1016/j.talanta.2020.121525

4. Directing a rational design of aptamer-based fluorescence anisotropy assay for sensitive detection of immunoglobulin E by site-specific binding study

Author:
Zhao, Qiang; Bai, Yunlong; Wang, Hailin
Source:
Talanta 2020 v.217 pp. 121018
ISSN:
0039-9140
Subject:
fluorescein,   etc   ; anisotropy; dissociation; fluorescence; immunoglobulin E; oligonucleotides;     Show all 6 Subjects
Abstract:
... Mapping aptamer-protein interactions is important for characterization and applications of aptamers against proteins. We describe here probing affinity interactions between aptamer and immunoglobulin E (IgE) with a fluorescence anisotropy (FA) approach using a series of aptamer probes having single fluorescein (FAM) label at individual nucleotide (A, C, T). Studies of binding between IgE and aptam ...
DOI:
10.1016/j.talanta.2020.121018

5. Aggregation-induced emission and solid fluorescence of fluorescein derivatives

Author:
Feng, Shumin; Gong, Shengyi; Feng, Guoqiang
Source:
Chemical communications 2020 v.56 no.16 pp. 2511-2513
ISSN:
1364-548X
Subject:
fluorescein,   etc   ; chemical compounds; chemical reactions; fluorescence;     Show all 4 Subjects
Abstract:
... We report herein for the first time that the aggregation-induced emission and strong solid fluorescence of fluorescein derivatives can be realized by slightly modifying their structure, which provides a new option for AIEgens and solid fluorescent materials. ...
DOI:
10.1039/c9cc09784h

6. Direct Measurement of Two-Photon Action Cross Section

Author:
Cabrera, Ricardo; Gabriel, Manuela; Estrada, Laura C.; Etchenique, Roberto
Source:
Analytical chemistry 2019 v.91 no.9 pp. 5968-5972
ISSN:
1520-6882
Subject:
fluorescein,   etc   ; microscopy; photolysis; serotonin;     Show all 4 Subjects
Abstract:
... We present a simple and fast methodology for measuring the two-photon (2P) action cross section of phototriggers. The method uses a standard 2P microscopy setup for both uncaging and detection and a set of lithographically made microcuvettes in order to reduce the total excitation volume and, thus, the photolysis time. The procedure does not need a standard and can be used for any caged compounds ...
DOI:
10.1021/acs.analchem.9b00392

7. Efficacy of fluorescent calcein mark retention and identification in endangered Colorado pikeminnow Ptychocheilus lucius

Author:
Gilbert, Eliza I.; Durst, Scott L.; Diver, Tracy A.; Mello, Hannah; Bertrand, Nick G.; Franssen, Nathan R.
Source:
Fisheries management and ecology 2020 v.27 no.3 pp. 258-269
ISSN:
0969-997X
Subject:
fluorescein,   etc   ; Ptychocheilus lucius; animal growth; endangered species; fish; probability;     Show all 6 Subjects
Abstract:
... Distinguishing hatchery‐reared fishes from wild conspecifics can be required to quantify the success of augmentation programmes. This study estimated the probability of identifying calcein‐marked, hatchery‐reared Colorado pikeminnow Ptychocheilus lucius Girard from external and internal structures. Both control and marked fish held in the laboratory were correctly identified 80% of the time after ...
DOI:
10.1111/fme.12408

8. Characterizing the impact of thermal gels on isotachophoresis in microfluidic devices

Author:
Ward, Cassandra L.; Linz, Thomas H.
Source:
Electrophoresis 2020 v.41 no.9 pp. 691-696
ISSN:
0173-0835
Subject:
fluorescein,   etc   ; chemical species; detection limit; electrophoresis; gels; liquids; polymers; temperature;     Show all 8 Subjects
Abstract:
... Thermally reversible Pluronic gels have been employed as separation matrices in microfluidic devices in the analysis of biological macromolecules. The phase of these gels can be tuned between liquid and solid states using temperature to vary fluidic resistance and alter peak resolution. Although separations in thermal gels have been characterized, their effect on isotachophoresis has not. This stu ...
DOI:
10.1002/elps.201900407

9. Cysteine specific bioconjugation with benzyl isothiocyanates

Author:
Petri, László; Szijj, Péter A.; Kelemen, Ádám; Imre, Tímea; Gömöry, Ágnes; Lee, Maximillian T. W.; Hegedűs, Krisztina; Ábrányi-Balogh, Péter; Chudasama, Vijay; Keserű, György Miklós
Source:
RSC advances 2020 v.10 no.25 pp. 14928-14936
ISSN:
2046-2069
Subject:
fluorescein,   etc   ; antibodies; benzyl isothiocyanate; cysteine; diagnostic techniques; disulfide bonds; pH;     Show all 7 Subjects
Abstract:
... Protein labelling has a wide variety of applications in medicinal chemistry and chemical biology. In addition to covalent inhibition, specific labelling of biomolecules with fluorescent dyes is important in both target discovery, validation and diagnostics. Our research was conducted through the fragment-based development of a new benzyl-isothiocyanate-activated fluorescent dye based on the fluore ...
DOI:
10.1039/d0ra02934c

10. A General Descriptor ΔE Enables the Quantitative Development of Luminescent Materials Based on Photoinduced Electron Transfer

Author:
Chi, Weijie; Chen, Jie; Liu, Wenjuan; Wang, Chao; Qi, Qingkai; Qiao, Qinglong; Tan, Tee Meng; Xiong, Kangming; Liu, Xiao; Kang, Keegan; Chang, Young-Tae; Xu, Zhaochao; Liu, Xiaogang
Source:
Journal of the American Chemical Society 2020 v.142 no.14 pp. 6777-6785
ISSN:
1520-5126
Subject:
fluorescein,   etc   ; bioimaging; biosensors; electron transfer; luminescence; prediction; rhodamines;     Show all 7 Subjects
Abstract:
... Photoinduced electron transfer (PET) is one of the most important mechanisms for developing fluorescent probes and biosensors. Quantitative prediction of the quantum yields of these probes and sensors is crucial to accelerate the rational development of novel PET-based functional materials. Herein, we developed a general descriptor (ΔE) for predicting the quantum yield of PET probes, with a thresh ...
DOI:
10.1021/jacs.0c01473

11. Spectrophotometric Determination of Copper(II) in Water based on Fluorescein Diacetate

Author:
Haishuang Jia,; Liu, Wenfang; Li, Na; Wang, Jun; Song, Youtao
Source:
Journal of analytical chemistry 2020 v.75 no.3 pp. 330-342
ISSN:
1061-9348
Subject:
fluorescein diacetate,   etc   ; cell membranes; copper; detection limit; hydrazones; lipids; medicine;     Show all 7 Subjects
Abstract:
... Two novel methods for spectrophotometric determination of Cu²⁺ ion using synthesized and characterized furfuraldehyde fluorescein diacetate hydrazone (FFDH) and furfuraldehyde fluorescein hydrazone (FFH) have been proposed. Addition of Cu²⁺ to FFDH and FFH solutions results in a rapid color change from colorless to dark yellow. Experimental results show that FFH and FFDH both can provide a rapid, ...
DOI:
10.1134/S1061934820030089

12. Spectral Distortions in Metal-Enhanced Fluorescence: Experimental Evidence for Ultra-Fast and Slow Transitions

Author:
Knoblauch, Rachael; Ben Hamo, Hilla; Marks, Robert; Geddes, Chris D.
Source:
Journal of physical chemistry 2020 v.124 no.8 pp. 4723-4737
ISSN:
1932-7455
Subject:
fluorescein,   etc   ; fluorescence; fluorescence emission spectroscopy; nanosilver; physical chemistry;     Show all 5 Subjects
Abstract:
... Metal-enhanced fluorescence (MEF) has become an increasingly important technology in recent years, with thorough research addressing the fundamentals of MEF. In many studies, spectral distortion is observed in the enhanced spectra as compared to free-space fluorescence emission profiles. Despite this observation, very little experimentation has hitherto been undertaken to investigate the mechanist ...
DOI:
10.1021/acs.jpcc.9b11055

13. Elucidating relationship between nitrous oxide emission and functional soil microbes from tropical lowland rice soil exposed to elevated CO2: A path modelling approach

Author:
Kumar, Anjani; Padhy, Soumya Ranjan; Das, Rasmita Rani; Shahid, Mohammad; Dash, Pradeep Kumar; Senapati, Ansuman; Panneerselvam, P.; Kumar, Upendra; Chatterjee, Dibyendu; Adak, Totan; Tripathi, Rahul; Nayak, P.K.; Nayak, A.K.
Source:
Agriculture, ecosystems & environment 2021 v.308 pp. 107268
ISSN:
0167-8809
Subject:
fluorescein diacetate,   etc   ; agriculture; carbon dioxide; carbon dioxide enrichment; denitrifying microorganisms; dynamics; ecosystems; environment; field experimentation; least squares; microbial activity; mineralization; models; nitrogen; nitrous oxide; nitrous oxide production; path analysis; population; prediction; rice; rice soils; soil microorganisms; urease; vegetative growth; water stress;     Show all 25 Subjects
Abstract:
... Understanding the effect of elevated atmospheric CO₂ on nitrous oxide (N₂O) emission and plant-microbe interactions in rice soils is of great significance for predicting the long-term response of rice ecosystems to elevated CO₂ concentration. Elevated atmospheric CO₂ concentration has the potential to augment rice production and alter the soil nitrogen (N) dynamics. Because N₂O emission is control ...
DOI:
10.1016/j.agee.2020.107268

14. Fluorescein propiolate: a propiolate-decorated fluorescent probe with remarkable selectivity towards cysteine

Author:
Karakuş, Erman; Sayar, Melike; Dartar, Suay; Kaya, Beraat Umur; Emrullahoğlu, Mustafa
Source:
Chemical communications 2019 v.55 no.34 pp. 4937-4940
ISSN:
1364-548X
Subject:
fluorescein,   etc   ; chemical reactions; cysteine; fluorescence;     Show all 4 Subjects
Abstract:
... A fluorescent probe decorated with an alkynyl ester unit (e.g. propiolate) displayed a selective turn-on type fluorescent response towards cysteine. Following a sequential addition–cyclisation pathway mediated by the addition of cysteine, the pre-fluorescent dye rapidly transformed into a new structure and induced a fluorescent response clearly observable with the naked eye. ...
DOI:
10.1039/c9cc01774g

15. Fluorescently labeled cellulose nanofibrils for detection and loss analysis

Author:
Reid, Michael S.; Karlsson, Maria; Abitbol, Tiffany
Source:
Carbohydrate polymers 2020 v.250 pp. 116943
ISSN:
0144-8617
Subject:
fluorescein,   etc   ; cellulose; cellulose nanofibers; cold; fluorescence; food contact surfaces; homogenization; leaching; paper; pulp; water;     Show all 11 Subjects
Abstract:
... Fluorescently labeled cellulose nanofibrils (CNFs) were used to evaluate CNF leaching from paper according to standard safety assays for food contact materials. Enzymatically pretreated pulp was first labeled with 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF), followed by homogenization to produce fluorescent CNFs of varying degrees of fibrillation. Labeling at the μmolar DTA ...
DOI:
10.1016/j.carbpol.2020.116943

16. A split aptamer sensing platform for highly sensitive detection of theophylline based on dual-color fluorescence colocalization and single molecule photobleaching

Author:
Liu, Shi Gang; Zhang, Dong; He, Yu; Gao, Wenli; Shi, Xingbo
Source:
Biosensors & bioelectronics 2020 v.166 pp. 112461
ISSN:
0956-5663
Subject:
fluorescein,   etc   ; RNA; aptasensors; detection limit; fluorescence; isothiocyanates; oligonucleotides; photobleaching; streptavidin; theophylline;     Show all 10 Subjects
Abstract:
... A new split aptamer sensing platform is developed for highly sensitive and selective detection of theophylline based on single molecule photobleaching (SMPB) technique. The sensing system contains two probes. One is formed by one streptavidin and four biotinylated RNA fragments labelled with fluorescein isothiocyanate (FITC). Each biotinylated RNA fragment contains two repeating aptamer fragments. ...
DOI:
10.1016/j.bios.2020.112461

17. A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET

Author:
Shi, Pan; Zhang, Yanan; Lv, Pei; Fang, Wei; Ling, Shenglong; Guo, Xiaoqi; Li, Dong; Liu, Sanling; Sun, Demeng; Zhang, Longhua; Liu, Dongsheng; Zheng, Ji-Shen; Tian, Changlin
Source:
Chemical communications 2020 v.56 no.51 pp. 6941-6944
ISSN:
1364-548X
Subject:
fluorescein,   etc   ; G-protein coupled receptors; G-proteins; chemical compounds; chemical reactions; energy transfer; fluorescence; signal transduction;     Show all 8 Subjects
Abstract:
... The dynamics of GPCRs (G protein-coupled receptors) coupling for cognate G proteins play a critical role in signal transduction. Herein, we reported a site-specifically labelled small-sized fluorescent pair 7-HC/FlAsH ((7-hydroxycoumarin-4-yl)-ethylglycine/fluorescein arsenical hairpin) for fluorescence lifetime based FRET (fluorescence resonance energy transfer) to reveal conformational differenc ...
DOI:
10.1039/d0cc02691c

18. Determining the critical micelle concentrations of cationic surfactants based on the visible-light-induced oxidase-like activity of fluorescein

Author:
Wei, Zixuan; Yi, Danyang; Hu, Xuemei; Sun, Chaoqun; Long, Yijuan; Zheng, Huzhi
Source:
Colloids and surfaces 2020 v.595 pp. 124698
ISSN:
0927-7757
Subject:
fluorescein,   etc   ; absorbance; aqueous solutions; catalytic activity; cationic surfactants; electrostatic interactions; enzymes; fluorescence; lighting; micelles; oxidation;     Show all 11 Subjects
Abstract:
... As the critical micelle concentration (CMC) is one of the essential parameters of surfactant, the determination of CMC value is of great significance. We explored a facile and novel strategy based on the visible-light-induced oxidase-like activity of fluorescein for determining the CMC value of cationic surfactants. Fluorescein as a visible-light-induced oxidase mimic can catalyze the change of 3, ...
DOI:
10.1016/j.colsurfa.2020.124698

19. Synthetic non-classical luminescence generation by enhanced silica nanophotonics based on nano-bio-FRET

Author:
Salinas, Carina; Amé, María Valeria; Bracamonte, A. Guillermo
Source:
RSC advances 2020 v.10 no.35 pp. 20620-20637
ISSN:
2046-2069
Subject:
fluorescein,   etc   ; Cyanobacteria; emissions; energy transfer; fluorescence; fluorescence microscopy; nanoparticles; rhodamines; silica; transmission electron microscopy;     Show all 10 Subjects
Abstract:
... Fluorescent silica nanoparticles (NPs–(SiO₂–Fluo)) were synthesized based on the classical Störber method for cyanobacteria labelling. Modified mono-coloured SiO₂ NPs with fluorescein (Fl) and rhodamine B (RhB) were obtained (NPs–(SiO₂–Fl) and NPs–(SiO₂–RhB)). Moreover, multi-coloured SiO₂ NPs, via the incorporation of both emitters (NPs–(SiO₂–RhB–Fl)), were tuned for optimal emissions and the bio ...
DOI:
10.1039/d0ra02939d

20. Quantitative analysis of the defects in CVD grown graphene by plasmon-enhanced Raman scattering

Author:
Liu, Yansheng; Feng, Huayu; Luo, Feng
Source:
Carbon 2020 v.161 pp. 153-161
ISSN:
0008-6223
Subject:
fluorescein,   etc   ; Raman spectroscopy; gold; graphene; nanocrystallites; nanogold; nanoparticles; quantitative analysis; vapors;     Show all 9 Subjects
Abstract:
... In this paper, a study of quantitative analysis of the defects in chemical vapor deposition (CVD) grown graphene through plasmon-enhanced Raman scattering has been performed. By designing and fabricating three-dimensional hybrid Au nano-particles/single-layer-graphene/Au nano-holes (Au NPs/SLG/Au NHs) structures, the defects induced Raman scattering signals of SLG have been extremely enhanced. The ...
DOI:
10.1016/j.carbon.2020.01.063