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1. An update on molecular counting in fluorescence microscopy

Author:
Hummert, Johan; Tashev, Stanimir Asenov; Herten, Dirk-Peter
Source:
The international journal of biochemistry & cell biology 2021 v.135 pp. 105978
ISSN:
1357-2725
Subject:
fluorescence; fluorescence microscopy,   etc   ; biochemistry;     Show all 3 Subject
Abstract:
... Quantitative assessment of protein complexes, such as receptor clusters in the context of cellular signalling, has become a pressing objective in cell biology. The advancements in the field of single molecule fluorescence microscopy have led to different approaches for counting protein copy numbers in various cellular structures. This has resulted in an increasing interest in robust calibration pr ...
DOI:
10.1016/j.biocel.2021.105978

2. Triple-Color STED Nanoscopy: Sampling Absorption Spectra Differences for Efficient Linear Species Unmixing

Author:
Gonzalez Pisfil, Mariano; Rohilla, Sumeet; König, Marcelle; Krämer, Benedikt; Patting, Matthias; Koberling, Felix; Erdmann, Rainer
Source:
TheJournal of physical chemistry 2021 v.125 no.22 pp. 5694-5705
ISSN:
1520-5207
Subject:
fluorescence; fluorescence microscopy,   etc   ; absorption; algorithms;     Show all 4 Subjects
Abstract:
... Stimulated emission depletion (STED) in confocal fluorescence microscopy enables a visualization of biological structures within cells far below the optical diffraction limit. To meet the demand in the field for simultaneous investigations of multiple species within a cell, a couple of different STED techniques have been proposed, each with their own challenges. By systemically exploiting spectral ...
DOI:
10.1021/acs.jpcb.0c11390

3. Dechloranes exhibit binding potency and activity to thyroid hormone receptors

Author:
Zhu, Jianqiao; Zhao, Lixia; Guo, Lianghong
Source:
Journal of environmental sciences (China) 2022 v.112 pp. 16-24
ISSN:
1001-0742
Subject:
fluorescence,   etc   ; humans; luciferase; reporter genes; risk; thyroid function; thyroid hormones; China;     Show all 8 Subjects
Abstract:
... Dechloranes are a group of halogenated flame retardants with a basic bicyclo[2.2.1]heptene, including Dechlorane Plus (DP), Dechlorane 602 (Dec 602), Dechlorane 603 (Dec 603) and Dechlorane 604 (Dec 604). A few epidemiological investigations and animal experiments have shown that DP exhibited thyroid-interfering effects. In the present study, we investigated whether DP and three other dechloranes ...
DOI:
10.1016/j.jes.2021.04.030

4. Number of galloyl moiety and intramolecular bonds in galloyl-based polyphenols affect their interaction with alpha-glucosidase

Author:
Cao, Junwei; Yan, Shaoqing; Xiao, Yao; Han, Lin; Sun, Lijun; Wang, Min
Source:
Food chemistry 2022 v.367 pp. 129846
ISSN:
0308-8146
Subject:
fluorescence,   etc   ; active sites; alpha-glucosidase; amino acids; ellagic acid; enzyme inhibition; food chemistry; hydrogen; moieties; polyphenols;     Show all 10 Subjects
Abstract:
... The inhibition of α-glucosidase by nine galloyl-based polyphenols with free and unfree galloyl moieties (GMs) was studied. The results show that the inhibitory activity increased with the free GM number increasing. For the compounds with unfree GMs, ellagic acid and hexahydroxydiphenoyl group contributed to the enzyme inhibition. Free GMs could bind not only with the active site of α-glucosidase ( ...
DOI:
10.1016/j.foodchem.2021.129846

5. Accelerated alkalinity regulation and long-term dry-wet aging durability for bauxite residue remediated with biomass pyrolysis

Author:
He, Zhexiang; Zhou, Jingju; Tang, Jianwei; Li, Chuxuan; Jiang, Jun; Chen, Wei; Zhu, Feng; Xue, Shengguo
Source:
Journal of environmental sciences (China) 2022 v.111 pp. 220-228
ISSN:
1001-0742
Subject:
fluorescence,   etc   ; alkalinity; bauxite; biomass; calcite; durability; fermentation; humic acids; pH stability; pyrolysis; remediation; soil formation; straw; China;     Show all 14 Subjects
Abstract:
... Biomass fermentation provides a potential route toward the ecological disposal for the bauxite residue (BR) with high alkalinity issues. However, how to accelerate the remediation of the alkaline problem with a long-term durability is still a big challenge. Herein, we investigated the acceleration of the decomposition of straw toward organic acid species via a pyrolysis strategy as well as the pH ...
DOI:
10.1016/j.jes.2021.03.021

6. Single-Molecule Tracking of Chromatin-Associated Proteins in the C. elegans Gonad

Author:
von Diezmann, Lexy; Rog, Ofer
Source:
TheJournal of physical chemistry 2021 v.125 no.23 pp. 6162-6170
ISSN:
1520-5207
Subject:
fluorescence; fluorescence microscopy,   etc   ; chromatin; meiosis; molecular dynamics;     Show all 5 Subjects
Abstract:
... Biomolecules are distributed within cells by molecular-scale diffusion and binding events that are invisible in standard fluorescence microscopy. These molecular search kinetics are key to understanding nuclear signaling and chromosome organization and can be directly observed by single-molecule tracking microscopy. Here, we report a method to track individual proteins within intact C. elegans gon ...
DOI:
10.1021/acs.jpcb.1c03040

7. Using constant-wavelength synchronous fluorescence spectroscopy in nanoparticle-based sensors: a minireview

Author:
Jouyban, Abolghasem; Rahimpour, Elaheh
Source:
Analytical methods 2021 v.13 no.8 pp. 968-973
ISSN:
1759-9679
Subject:
fluorescence; fluorescence emission spectroscopy,   etc   ; sensors (equipment);     Show all 3 Subject
Abstract:
... This study focuses on optical nanosensors based on constant-wavelength synchronous fluorescence spectroscopy (SFS) and reviews their applications for analysis purposes in the last few decades. In comparison to conventional fluorescence, SFS shows a higher selectivity owing to the narrowing of spectral bands and the simplification of spectra. The reported SFS-based nanosensors are classified based ...
DOI:
10.1039/d0ay02096f

8. Lasting Tracking and Rapid Discrimination of Live Gram-Positive Bacteria by Peptidoglycan-Targeting Carbon Quantum Dots

Author:
Yan, Chaoren; Wang, Chaoli; Hou, Tongtong; Guan, Ping; Qiao, Youbei; Guo, Liulong; Teng, Yonggang; Hu, Xiaoling; Wu, Hong
Source:
ACS applied materials & interfaces 2021 v.13 no.1 pp. 1277-1287
ISSN:
1944-8252
Subject:
fluorescence; fluorescence microscopy,   etc   ; bacterial infections; viability;     Show all 4 Subjects
Abstract:
... Selective discrimination and lasting tracking of live bacteria are primary steps for microbiology research and treatment of bacterial infection. However, conventional detection methods, such as the gold standard of Gram staining, are being challenged under actual test conditions. Herein, we provided a novel method, namely, three excitation peaks and single-color emission carbon quantum dots (T-SCQ ...
DOI:
10.1021/acsami.0c19651

9. Impact of pulsating flows on particle deposition in forward osmosis with spacers

Author:
Kastl, Andreas; Bar-Zeev, Edo; Spinnler, Markus; Sattelmayer, Thomas
Source:
Journal of membrane science 2021 v.635 pp. 119444
ISSN:
0376-7388
Subject:
fluorescence microscopy,   etc   ; osmosis; polystyrenes;     Show all 3 Subjects
Abstract:
... Fouling continues to be a limiting factor in membrane processes. Pulsating feed flows have the potential to decrease fouling while maintaining a constant pressure drop over the module. This study quantifies the impact of pulsating flows on particle deposition in a forward osmosis system with spacers at a mean crossflow velocity of 14 cm s⁻¹ and permeate water flux of 18 L m⁻² h⁻¹. Carboxylate modi ...
DOI:
10.1016/j.memsci.2021.119444

10. Carbon dots with dual emission: A versatile sensing platform for rapid assay of Cr (VI)

Author:
Jia, Mingyan; Peng, Lulin; Yang, Maohua; Wei, Huangzhao; Zhang, Mei; Wang, Yu
Source:
Carbon 2021 v.182 pp. 42-50
ISSN:
0008-6223
Subject:
fluorescence,   etc   ; absorption; chemical species;     Show all 3 Subjects
Abstract:
... In this article, we show that the conventional boron-nitrogen co-doped carbon dots (BNCDs) exhibit unique fluorescent ratiometricity in the detection of Cr (VI) due to their unique dual emission system. The 3D fluorescence spectrum study reveals that besides the commonplace emission centered at (360, 465) nm in blue fluorescence region, another center which may be corresponding to N-contain conjug ...
DOI:
10.1016/j.carbon.2021.05.050

11. Facile fabrication of luminescent rare-earth-doped PS/AA composites for anti-counterfeiting applications

Author:
Wang, Qing; Xie, Dong; Chen, Junjia; Liu, Guang; Yu, Mingguang
Source:
Journal of materials science 2021 v.56 no.23 pp. 13146-13155
ISSN:
0022-2461
Subject:
fluorescence,   etc   ; microparticles; photoluminescence; polymerization;     Show all 4 Subjects
Abstract:
... Herein, a facile method to develop luminescent PS/AA/Rare-Earth-doped composites was presented. The PS/AA microspheres were first carried out via one-pot dispersion polymerization. Then, the PS/AA microspheres bearing free carboxyl groups were used as templates for surface chemical deposition of rare earth ions forming lanthanide-based nanocrystal-doped PS/AA composites. The morphologies and struc ...
DOI:
10.1007/s10853-021-06133-4

12. Neutral reducing agents supported on merrifield resin obtained by solid phase organic synthesis

Author:
Aguilar-Martínez, Milagros; Vargas-Durazo, Judas Tadeo; Ochoa-Terán, Adrián; Santacruz-Ortega, Hisila; Ochoa-Lara, Karen; Zizumbo-López, Arturo; Gálvez-Ruíz, Juan Carlos
Source:
Tetrahedron 2021 v.91 pp. 132195
ISSN:
0040-4020
Subject:
fluorescence,   etc   ; borohydrides; thermogravimetry;     Show all 3 Subjects
Abstract:
... An efficient methodology for the synthesis of neutral reducing agents supported on Merrifield resin has been developed. The process was carried out under mild conditions, varying the linker length, the bidentate chelating amine and the alkali metal tetrahydroborate. Fluorescence and infrared spectroscopies and thermogravimetric analysis were used for monitoring the chemical modifications. The deve ...
DOI:
10.1016/j.tet.2021.132195

13. Measurement of mercury with highly selective fluorescent chemoprobe by carbon dots and silver nanoparticles

Author:
Zhou, Qingxiang; Yongli Liu,; Wu, Yalin; Li, Zhi; Li, Yanhui; Liu, Menghua; Qu, Tongxu; Chen, Chunmao
Source:
Chemosphere 2021 v.274 pp. 129959
ISSN:
0045-6535
Subject:
fluorescence,   etc   ; mercury; nanosilver;     Show all 3 Subjects
Abstract:
... This work describes a novel fluorescent chemoprobe that uses carbon dots and silver nanoparticles (AgNPs) to monitor mercury ions in aqueous samples attributed to the principle of inner filter effect. The fluorescent response signal of the carbon dots is diminished by AgNPs, attributed to inner filter effect, and is restored with the addition of Hg²⁺. The fluorescent chemoprobe was specific over t ...
DOI:
10.1016/j.chemosphere.2021.129959

14. Tough Bioinspired Composites That Self-Report Damage

Author:
Magrini, Tommaso; Kiebala, Derek; Grimm, Dominique; Nelson, Anna; Schrettl, Stephen; Bouville, Florian; Weder, Christoph; Studart, André R.
Source:
ACS applied materials & interfaces 2021 v.13 no.23 pp. 27481-27490
ISSN:
1944-8252
Subject:
fluorescence,   etc   ; color; polymers;     Show all 3 Subjects
Abstract:
... The increasing use of lightweight composite materials in structural applications requires the development of new damage monitoring technologies to ensure their safe use and prevent accidents. Although several molecular strategies have been proposed to report damage in polymers through mechanochromic responses, these approaches have not yet been translated into lightweight bioinspired composites fo ...
DOI:
10.1021/acsami.1c05964

15. Turn-folding in fluorescent anthracene-substituted cyclopenta[d]isoxazoline short peptides

Author:
Leusciatti, Marco; Mannucci, Barbara; Recca, Teresa; Quadrelli, Paolo
Source:
RSC advances 2021 v.11 no.32 pp. 19551-19559
ISSN:
2046-2069
Subject:
fluorescence,   etc   ; amino acids; peptides;     Show all 3 Subjects
Abstract:
... Cyclopenta[d]isoxazoline aminols were used for the synthesis of β-turn mimics. The peptide chain choice ascertained the influence of their structural features on the applicability/reliability/robustness of these scaffolds as β-turn inducers and their limitations. The amino acid selection as well as steric demands can favor or disfavor the structure folding and the correct design of the peptide cha ...
DOI:
10.1039/d1ra03685h

16. Fe3+ sensitivity fluorescence sensor from β-cyclodextrin-enhanced Eu3+ luminescence aggregates

Author:
Wang, Jing; Wang, Tao; Hu, Yimeng; Zhang, Xiaonan; Ma, Yanyan; Lv, Hongmin; Xu, Shanshan; Wang, Yinglong; Jiang, Zike
Source:
Journal of materials science 2021 v.56 no.18 pp. 10979-10989
ISSN:
0022-2461
Subject:
fluorescence,   etc   ; aqueous solutions; ligands;     Show all 3 Subjects
Abstract:
... In this work, novel β-cyclodextrin-enhanced Eu³⁺ luminescence aggregates (CELAs) with bright red fluorescence of Eu³⁺ are prepared. These CELAs exhibit the quick response and highly selective sensitivity at the concentration of 50 µM Fe³⁺. A series of ligands (such as TTA, phen, β-cyclodextrin) sensitizes the luminescence of Eu³⁺ (the “antenna effect”), and metal ions–ligands interactions can diff ...
DOI:
10.1007/s10853-021-05961-8

17. Mitochondria selective trackers for long-term imaging based on readily accessible neutral BODIPYs

Author:
Ramos-Torres, Ágata; Avellanal-Zaballa, Edurne; García-Garrido, Fernando; Fernández-Martínez, Ana B.; Prieto-Castañeda, Alejandro; Agarrabeitia, Antonia R.; Bañuelos, Jorge; García-Moreno, Inmaculada; Lucio-Cazaña, Francisco-Javier; Ortiz, María J.
Source:
Chemical communications 2021 v.57 no.43 pp. 5318-5321
ISSN:
1364-548X
Subject:
fluorescence,   etc   ; mitochondria; models;     Show all 3 Subjects
Abstract:
... We report the design of a new model based on a small neutral 8-aryl-3-formylBODIPY and its suitability to develop privileged highly bright and photostable fluorescent probes for selective and, more importantly, covalent staining of mitochondria. ...
DOI:
10.1039/d1cc00451d

18. Visualization of third-level information in latent fingerprints by a new fluorogenic l-tyrosine analogue

Author:
Singh, Harminder; Verma, Sandeep
Source:
Chemical communications 2021 v.57 no.43 pp. 5290-5293
ISSN:
1364-548X
Subject:
fluorescence,   etc   ; peptides; tyrosine;     Show all 3 Subjects
Abstract:
... Incorporation of fluorescent α-amino acids in peptide/protein sequences, at desired positions, is eminently useful for non-invasive detection of cellular events, without impacting their native properties. As an extension to such an approach, we describe the design of two stable, fluorescent l-tyrosine analogs, FHBY and BHBY, exhibiting photophysical properties associated with the AIE-coupled ESIPT ...
DOI:
10.1039/d1cc01910d

19. Donor–Acceptor Conjugated Polymers with Efficient Thermally Activated Delayed Fluorescence: Random versus Alternative Polymerization

Author:
Li, Xue; Rao, Jiancheng; Yang, Liuqing; Zhao, Lei; Wang, Shumeng; Tian, Hongkun; Ding, Junqiao; Wang, Lixiang
Source:
Macromolecules 2021 v.54 no.11 pp. 5260-5266
ISSN:
1520-5835
Subject:
fluorescence,   etc   ; energy; polymerization; polymers;     Show all 4 Subjects
Abstract:
... Random polymerization is newly proposed for the achievement of efficient thermally activated delayed fluorescence (TADF) in donor–acceptor (D–A) conjugated polymers. As a proof of concept, a series of random polymers named Ran-P(Ac-TRZ02) ∼ Ran-P(Ac-TRZ20) have been designed and synthesized based on the donor N-alkyl acridine (Ac) and the acceptor triphenyltriazine (TRZ) as the major and minor com ...
DOI:
10.1021/acs.macromol.1c00188

20. Nonconjugated Triptycene-Spaced Donor–Acceptor-Type Emitters Showing Thermally Activated Delayed Fluorescence via Both Intra- and Intermolecular Charge-Transfer Transitions

Author:
Dai, Gaole; Zhang, Ming; Wang, Kai; Fan, Xiaochun; Shi, Yizhong; Sun, Dianming; Liu, Wei; Chen, Jiaxiong; Yu, Jia; Ou, Xuemei; Xiong, Shiyun; Zheng, Caijun; Zhang, Xiaohong
Source:
ACS applied materials & interfaces 2021 v.13 no.21 pp. 25193-25201
ISSN:
1944-8252
Subject:
fluorescence,   etc   ; electrochemiluminescence; moieties;     Show all 3 Subjects
Abstract:
... Thermally activated delayed fluorescence (TADF) emitters have aroused considerable attention, particularly for their great potential in organic light-emitting diodes (OLEDs). In typical TADF molecules, intramolecular charge transfer (CT) between electron-donor (D) and electron-acceptor (A) moieties is the dominant transition. Actually, CT transitions can possibly occur between different molecules ...
DOI:
10.1021/acsami.1c05646