U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject "fluorescent antibody technique" Remove constraint Subject: "fluorescent antibody technique"
Start Over

Search

8,616  Search Results

« Previous | 1 - 20 of 8,616 |
Number of results to display per page

Search Results

1. The crude extract from the flowers of Trollius chinensis Bunge exerts anti-influenza virus effects through modulation of the TLR3 signaling pathway

Author:
Yuxi Liang; Xiaoli Liu; Jingyan Hu; Songli Huang; Xin Ma; Xiaoyan Liu; Rufeng Wang; Xiuhua Hu
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115743
ISSN:
0378-8741
Subject:
fluorescent antibody technique,   etc   ; Influenza A virus; Oriental traditional medicine; Trollius chinensis; Western blotting; blood; cytoplasm; epithelium; lungs; nasal cavity; pharmacology; pharyngitis; pneumonia; spleen; therapeutics; viruses;     Show all 16 Subjects
Abstract:
... The flowers of Trollius chinensis Bunge (Ranunculaceae) is a traditional Chinese medicine used to treat various inflammatory diseases, including upper respiratory infections, chronic tonsillitis, and pharyngitis. Recently, there has been growing research on the antiviral role of the flowers of T. chinensis Bunge. However, little is known about its anti-influenza virus effects and the underlying me ...
DOI:
10.1016/j.jep.2022.115743

2. Quantitative detection of malachite green in sediment by a time-resolved immunofluorescence method combined with a portable 3D printing equipment platform

Author:
Xinchi Wang; Tingting Yang; Xi Chen; Longxiang Fang; Yong Yang; Guoqing Cao; Haitao Zhang; Alex Bogere; Shunlong Meng; Jiazhang Chen; Chao Song
Source:
Science of the total environment 2023 v.855 pp. 158897
ISSN:
0048-9697
Subject:
fluorescent antibody technique,   etc   ; antibodies; aquaculture; detection limit; drugs; environment; equations; europium; fisheries; fluorescence; immune response; malachite green; microparticles; mobile telephones; oxidation; qualitative analysis; quantitative analysis; rapid methods; regression analysis; sediments;     Show all 20 Subjects
Abstract:
... Rapid detection technology of aquaculture fishery drug residues is needed to supplement large-scale instrument methods. To do this, the time-resolved fluorescence immunoassay (TRFIA) method and portable three-dimensional (3D) printing equipment platform were used, in combination with smartphones, to detect malachite green (MG) in pond sediments. The TRFIA was coupled to MG monoclonal antibodies (m ...
DOI:
10.1016/j.scitotenv.2022.158897

3. Atractylodes lancea and Magnolia officinalis combination protects against high fructose-impaired insulin signaling in glomerular podocytes through upregulating Sirt1 to inhibit p53-driven miR-221

Author:
Jie Yang; Zi-Xuan Wang; Lei Fang; Tu-Shuai Li; Zhi-Hong Liu; Ying Pan; Ling-Dong Kong
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115688
ISSN:
0378-8741
Subject:
fluorescent antibody technique,   etc   ; Atractylodes lancea; Magnolia officinalis; Oriental traditional medicine; Western blotting; adiponectin receptors; agonists; animal models; diet; fructose; humans; insulin; insulin resistance; kidneys; liquid chromatography; non-specific serine/threonine protein kinase; pharmacology; rats; tandem mass spectrometry; therapeutics; thermosensitivity;     Show all 21 Subjects
Abstract:
... In traditional Chinese medicine, a long term of improper diet causes the Dampness and disturbs Zang-Fu's functions including Kidney deficiency. Atractylodes lancea (Atr) and Magnolia officinalis (Mag) as a famous herb pair are commonly used to transform Dampness, with kidney protection. To explore how Atr and Mag protected against insulin signaling impairment in glomerular podocytes induced by hig ...
DOI:
10.1016/j.jep.2022.115688

4. Xianglian Pill attenuates ulcerative colitis through TLR4/MyD88/NF-κB signaling pathway

Author:
Yuxin Dai; Qiulu Lu; Peiyi Li; Junyu Zhu; Jiaxin Jiang; Tong Zhao; Yue Hu; Kang Ding; Min Zhao
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115690
ISSN:
0378-8741
Subject:
fluorescent antibody technique,   etc   ; Oriental traditional medicine; Western blotting; blood serum; colon; dextran sulfate; enzyme activity; enzyme-linked immunosorbent assay; histopathology; interleukin-18; interleukin-6; liquid chromatography; models; myeloperoxidase; necrosis; neoplasms; protein domains; quantitative polymerase chain reaction; salicylic acid; ubiquitination; ulcerative colitis;     Show all 21 Subjects
Abstract:
... Xianglian Pill (XLP) is a classical Chinese medicine prescription applied for controlling ulcerative colitis (UC). Whereas, the underlying mechanism remains unclear. The present work was aimed to investigate the mechanism of XLP in dextran sulfate sodium (DSS)-induced UC via the Toll Like Receptor 4 (TLR4)/Myeloid Differentiation factor 88 (MyD88)/Nuclear Factor kappa-B (NF-κB) signaling in mice.  ...
DOI:
10.1016/j.jep.2022.115690

5. 23-O-acetylshengmanol-3-O-α-L-arabinoside alleviates lipopolysaccharide-induced acute lung injury through inhibiting IκB/NF-κB and MAPK/AP-1 signaling pathways

Author:
Chunyan Chen; Lin Li; Xiaomei Liu; Deqin Zhang; Yang Liu; Yuhong Li
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115725
ISSN:
0378-8741
Subject:
fluorescent antibody technique,   etc   ; Actaea cimicifuga; Oriental traditional medicine; Western blotting; blood serum; cytotoxicity; edema; enzyme-linked immunosorbent assay; eosin; genes; glycosides; histopathology; interleukin-6; lipopolysaccharides; lung function; lungs; macrophages; models; nitric oxide; pharyngitis; phosphorylation; plethysmography; protein content; rhizomes; therapeutics; toxicity testing; triterpenoids;     Show all 27 Subjects
Abstract:
... Cimicifuga foetida L. is a well-established traditional Chinese medicine with heat-clearing and detoxifying effects and has good therapeutic effect on oral mucosal ulcer and pharyngitis. The rhizome of this herb is rich in triterpenoid glycosides, including 23-O-acetylshengmanol-3-o-α-L-arabinoside (DA). Whether and how DA attenuates acute lung injury (ALI) are unclear. Accordingly, we focused on ...
DOI:
10.1016/j.jep.2022.115725

6. Transcriptome analysis and pattern recognition receptors (PRRs) identification in different tissues of adult Pacific oysters infected with Vibrio parahaemolyticus

Author:
Petcharat Namwong; Shuang Wang; Qing Kong; Haijin Mou; Lei Ma; Prapansak Srisapoome
Source:
Aquaculture 2023 v.562 pp. 738824
ISSN:
0044-8486
Subject:
fluorescent antibody technique,   etc   ; Crassostrea gigas; Gram-negative bacteria; Vibrio parahaemolyticus; adults; aquaculture industry; bacterial adhesion; bacterial infections; carbohydrate binding; cytoskeleton; data collection; epithelium; food sanitation; galectins; gene expression; gene expression regulation; genes; host-pathogen relationships; macrophages; mannose; oxidative stress; pathogens; peptidoglycans; peroxidase; phagocytosis; recombinant proteins; shellfish culture; transcriptomics;     Show all 28 Subjects
Abstract:
... Contamination of V. parahaemolyticus in raw product and culture areas of Crassostrea gigas has been a concern in food hygiene and infectious diseases in the shellfish aquaculture industry for decades. Although numerous researchers have presently objective of decreasing pathogen microorganism contaminated load in raw oysters or genetics improvement for prevention, the in-depth information at the mo ...
DOI:
10.1016/j.aquaculture.2022.738824

7. Integration of Multiple, Diverse Methods to Identify Biologically Significant Marker Genes

Author:
Christopher P. Chaney; Keri A. Drake; Thomas J. Carroll
Source:
Journal of molecular biology 2022 v.434 no.19 pp. 167754
ISSN:
0022-2836
Subject:
fluorescent antibody technique,   etc   ; gene expression regulation; molecular biology;     Show all 3 Subjects
Abstract:
... Identification of genes that reliably mark distinct cell types is key to leveraging single-cell RNA sequencing to better understand organismal biology. Such genes are usually chosen by measurement of differential expression between groups of cells and selecting those with the greatest magnitude or most statistically significant change. Many methods have been developed for performing such analyses, ...
DOI:
10.1016/j.jmb.2022.167754

8. Successful Repigmentation of Full-Thickness Wound Healing in Fraser’s Dolphins (Lagenodelphis hosei)

Author:
Chen-Yi Su; Hao-Ven Wang; Michael W. Hughes; Tzu-Yu Liu; Cheng-Ming Chuong; Wei-Cheng Yang
Source:
Animals 2022 v.12 no.12 pp. -
ISSN:
2076-2615
Subject:
fluorescent antibody technique,   etc   ; Cetacea; immunohistochemistry; melanin; melanocytes; pigmentation;     Show all 6 Subjects
Abstract:
... Fraser’s dolphins (Lagenodelphis hosei) exhibit the capability to restore nearly normal pigmentation after full-thickness wounding. However, the association among melanocytes, melanin and skin pigmentation during wound healing in cetaceans has yet to be addressed. Here, the number of melanocytes and the distribution of melanocytes and melanin in different-colored skin and different wound-healing s ...
DOI:
10.3390/ani12121482

9. Serological Evidence of Exposure to Onyong-Nyong and Chikungunya Viruses in Febrile Patients of Rural Taita-Taveta County and Urban Kibera Informal Settlement in Nairobi, Kenya

Author:
Moses Muia Masika; Essi M. Korhonen; Teemu Smura; Ruut Uusitalo; Joseph Ogola; Dufton Mwaengo; Anne J. Jääskeläinen; Hussein Alburkat; Yong-Dae Gwon; Magnus Evander; Omu Anzala; Olli Vapalahti; Eili Huhtamo
Source:
Viruses 2022 v.14 no.6 pp. -
ISSN:
1999-4915
Subject:
fluorescent antibody technique,   etc   ; Sindbis virus; blood; neutralization; seroprevalence; Kenya;     Show all 6 Subjects
Abstract:
... Several alphaviruses, such as chikungunya (CHIKV) and Onyong-nyong (ONNV), are endemic in Kenya and often cause outbreaks in different parts of the country. We assessed the seroprevalence of alphaviruses in patients with acute febrile illness in two geographically distant areas in Kenya with no previous record of alphavirus outbreaks. Blood samples were collected from febrile patients in health fa ...
DOI:
10.3390/v14061286

10. Mechanically induced histochemical and structural damage in the annulus fibrosus and cartilaginous endplate: a multi-colour immunofluorescence analysis

Author:
Jackie D. Zehr; Fasih Ahmad Rahman; Jack P. Callaghan; Joe Quadrilatero
Source:
Cell and tissue research 2022 v.390 no.1 pp. 59-70
ISSN:
0302-766X
Subject:
fluorescent antibody technique,   etc   ; byproducts; collagen; fluorescence; histochemistry; research; swine;     Show all 7 Subjects
Abstract:
... The annulus fibrosus (AF) and endplate (EP) are collagenous spine tissues that are frequently injured due to gradual mechanical overload. Macroscopic injuries to these tissues are typically a by-product of microdamage accumulation. Many existing histochemistry and biochemistry techniques are used to examine microdamage in the AF and EP; however, there are several limitations when used in isolation ...
DOI:
10.1007/s00441-022-03649-2

11. Expression of FMRpolyG in Peripheral Blood Mononuclear Cells of Women with Fragile X Mental Retardation 1 Gene Premutation

Author:
Xuan Phuoc Nguyen; Adriana Vilkaite; Birgitta Messmer; Jens E. Dietrich; Katrin Hinderhofer; Knut Schäkel; Thomas Strowitzki; Julia Rehnitz
Source:
Genes 2022 v.13 no.3 pp. -
ISSN:
2073-4425
Subject:
fluorescent antibody technique,   etc   ; alleles; amenorrhea; behavior disorders; flow cytometry; toxicity;     Show all 6 Subjects
Abstract:
... Fragile X-associated primary ovarian insufficiency (FXPOI) is characterized by oligo/amenorrhea and hypergonadotropic hypogonadism and is caused by the expansion of the CGG repeat in the 5′UTR of Fragile X Mental Retardation 1 (FMR1). Approximately 20% of women carrying an FMR1 premutation (PM) allele (55–200 CGG repeat) develop FXPOI. Repeat Associated Non-AUG (RAN)-translation dependent on the v ...
DOI:
10.3390/genes13030451

12. Seroprevalence to Rabies Virus in Wildlife in Brazil

Author:
Felipe Fornazari; Karin Corrêa Scheffer; Sandriana dos Ramos Silva; Karina Ribeiro da Silva; Adriana Cândido Rodrigues; Carlos Roberto Teixeira; Luna Scarpari Rolim; Helio Langoni
Source:
Journal of wildlife diseases 2022 v.58 no.2 pp. 431-435
ISSN:
0090-3558
Subject:
fluorescent antibody technique,   etc   ; Rabies lyssavirus; blood serum; seroprevalence; wildlife; Brazil;     Show all 6 Subjects
Abstract:
... Serum samples of 638 free-ranging wild mammals from São Paulo state, Brazil, were tested for neutralizing antibodies against rabies virus by the rapid fluorescent focus inhibition test. Overall seroprevalence was 1.7% among 24 species surveyed, with individuals of six species having positive results indicating exposure to rabies virus. ...
DOI:
10.7589/JWD-D-21-00065

13. Single-cell variations in the expression of codominant alleles A and B on RBC of AB blood group individuals

Author:
ANJALI BAJPAI; VIDYA JONNALAGADDA; BADANAPURAM SRIDEVI; PATHMA MUTHUKOTTIAPPAN; RACHEL A. JESUDASAN
Source:
Journal of genetics 2022 v.101 no.2 pp. 36
ISSN:
0973-7731
Subject:
fluorescent antibody technique,   etc   ; antigens; blood groups; normal distribution; phenotypic variation;     Show all 5 Subjects
Abstract:
... A key question in biology is whether all cells of a given ‘cell type’ within an individual have more or less the same phenotype, especially in relation to nonimprinted autosomal loci. Some studies have shown differential allelic expression of autosomal genes to confer phenotypic variability at the individual cell level. Here, we report the amount of A and B histo-blood group antigens, products of ...
DOI:
10.1007/s12041-022-01376-9

14. A cost-effective sample pooling strategy for line blot assay in detecting onconeural antibodies

Author:
Renfen Chen; Irene Luo; Megan Rae; Shirley Huang
Source:
Journal of immunological methods 2022 v.503 pp. 113235
ISSN:
0022-1759
Subject:
fluorescent antibody technique,   etc   ; antigens; autoantibodies; blood serum; cost effectiveness; labor;     Show all 6 Subjects
Abstract:
... Onconeural antibodies are a group of autoantibodies present in patients with paraneoplastic syndromes (PNS), and are indicative for underlying malignancies. The use of indirect immunofluorescence assay (IFA) as the sole screening method for onconeural antibodies without line blot assay (LBA) could potentially miss a significant population of patients with PNS. However, testing each serum individua ...
DOI:
10.1016/j.jim.2022.113235

15. A new method for primary culture of microglia in rats with spinal cord injury

Author:
Chengjie Wu; Yalan Pan; Lining Wang; Mengmin Liu; Mao Wu; Jianwei Wang; Guanglu Yang; Yang Guo; Yong Ma
Source:
Biochemical and biophysical research communications 2022 v.599 pp. 63-68
ISSN:
0006-291X
Subject:
fluorescent antibody technique,   etc   ; animal injuries; models; neuroglia; research; spinal cord;     Show all 6 Subjects
Abstract:
... At present, the primary culture method of microglia is complicated, and the culture of spinal cord microglia is rare, so we will explore to establish a new and efficient primary culture method of microglia in rats with spinal cord injury (SCI). The SCI model of SD rats was established by modified A11en′s method, and the model of SCI was performed on 1 d, 3 d, 7 d and 14 d respectively. Then the in ...
DOI:
10.1016/j.bbrc.2022.02.027

16. Single cell analysis of PANoptosome cell death complexes through an expansion microscopy method

Author:
Yaqiu Wang; Nagakannan Pandian; Joo-Hui Han; Balamurugan Sundaram; SangJoon Lee; Rajendra Karki; Clifford S. Guy; Thirumala-Devi Kanneganti
Source:
Cellular and molecular life sciences 2022 v.79 no.10 pp. 531
ISSN:
1420-682X
Subject:
fluorescent antibody technique,   etc   ; caspase-8; cytokines; inflammasomes; innate immunity; mice; microscopy; pyroptosis;     Show all 8 Subjects
Abstract:
... In response to infection or sterile insults, inflammatory programmed cell death is an essential component of the innate immune response to remove infected or damaged cells. PANoptosis is a unique innate immune inflammatory cell death pathway regulated by multifaceted macromolecular complexes called PANoptosomes, which integrate components from other cell death pathways. Growing evidence shows that ...
DOI:
10.1007/s00018-022-04564-z

17. A Comparative Evaluation of the Estimation of Rabies Virus Antibodies among Free-Roaming, Vaccinated Dogs in Bengaluru, India

Author:
Lekshmi J. Das; Shrikrishna Isloor; Alur Kotrappa Santosh; Avinash Bhat; Ramakrishnaiah Sharada; Doddamane Rathnamma; Belamaranahally Muniveerappa Veeregowda; Konanduru Lingappa Phaniraj; Nageshkumar Abhijit Kumar; Abi T. Vanak
Source:
Viruses 2022 v.14 no.3 pp. -
ISSN:
1999-4915
Subject:
fluorescent antibody technique,   etc   ; Rabies lyssavirus; blood serum; dogs; immunogenicity; rabies; vaccination; India;     Show all 8 Subjects
Abstract:
... Vaccination is the practical solution for the prevention of rabies in dogs. Assessment of the immunogenicity of vaccination includes estimation of specific rabies virus neutralizing antibodies (VNA) in the target species. We undertook a study to estimate the levels of VNA in free-roaming dogs with a history of rabies vaccination in Bengaluru city, India. We compared the rapid fluorescent focus inh ...
DOI:
10.3390/v14030484

18. Differential distribution and genetic determination of eccrine sweat glands and hair follicles in the volar skin of C57BL/6 mice and SD rats

Author:
Zixiu Chen; Junhong Zhao; Yongjing Yan; Lei Zhang; Lijie Du; Xiang Liu; Manxiu Cao; Cangyu Wang; Yue Tang; Haihong Li
Source:
BMC veterinary research 2022 v.18 no.1 pp. 316
ISSN:
1746-6148
Subject:
fluorescent antibody technique,   etc   ; body temperature; eosin; genes; humans; sweat; symporters; veterinary medicine;     Show all 8 Subjects
Abstract:
... BACKGROUND: Eccrine sweat glands (ESGs) and hair follicles (HFs) are the prominent skin appendages regulating human body temperature. C57BL/6 mice and Sprague–Dawley (SD) rats are the most commonly used model animals for studying ESGs and HFs. Previous studies have shown the distribution of ESGs and HFs in volar hindfeet of C57BL/6 mice, but there are few or no reports on the distribution of ESGs ...
DOI:
10.1186/s12917-022-03416-z

19. Importin alpha 1 is required for the nucleus entry of Fowl Adenovirus serotype 4 Fiber-1 protein

Author:
Ruiling Huang; Qing He; Shaohua Lu; Menghan Yan; Lihui Xu; Quanxi Wang
Source:
Veterinary microbiology 2022 v.266 pp. 109351
ISSN:
0378-1135
Subject:
fluorescent antibody technique,   etc   ; Adenoviridae; cytoplasm; fluorescence; importins; microbiology; serotypes; structural proteins; transfection;     Show all 9 Subjects
Abstract:
... Fiber-1 protein (F1) is the structural protein of Fowl Adenovirus serotype 4 (FAdV-4), which could recondite the receptors of host cytomembrane. In this study, we firstly determined that F1 protein located in nucleus of LMH cells after infection with FAdV-4. We additionally revealed that F1 protein had a classic NLS, and the NLS was required for F1 nucleus entry, which was intently associated to t ...
DOI:
10.1016/j.vetmic.2022.109351

20. Neuroprotective effect of pseudoginsenoside-F11 on permanent cerebral ischemia in rats by regulating calpain activity and NR2A submit-mediated AKT-CREB signaling pathways

Author:
Yueyang Liu; Xiaoxiao Fu; Yuhuan Liu; Tianyu Zhang; Peirui Cui; Saiqian Wang; Liting Liu; Zongjuan Hou; Huiyang Wang; Yang Zhao; Zinv Zhang; Haotian Zhang; Chunfu Wu; Jingyu Yang
Source:
Phytomedicine 2022 v.96 pp. 153847
ISSN:
0944-7113
Subject:
fluorescent antibody technique,   etc   ; antagonists; calpain; cortex; death; ischemia; neurons; neuroprotective effect; stroke;     Show all 9 Subjects
Abstract:
... N-methyl-d-aspartate receptors (NMDARs) have been demonstrated to play central roles in stroke pathology and recovery, including dual roles in promoting either neuronal survival or death with their different subtypes and locations.We have previously demonstrated that pseudoginsenoside-F11 (PF11) can provide long-term neuroprotective effects on transient and permanent ischemic stroke-induced neuron ...
DOI:
10.1016/j.phymed.2021.153847