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... Food quality and safety has become a subject of major concern for authorities and professionals in the food supply chain. Rapid methods, particularly biosensors, have exceptional specificity and sensitivity, rapid response times, low cost, relatively compact size, and are user friendly to operate. Cell-based biosensors are portable, and provide the biological activity of the analyte suitable for a ...
... Listeria monocytogenes is a major foodborne pathogen. Testing multiple portions of the same final product is often required to verify the effectiveness of a food safety management system. Therefore, it will be advantageous to the laboratories to combine these test portions and process as one sample. However, combining samples for analysis, i.e., pooling, can be done only if there is no negative im ...
... Rapid and efficient detection of pathogenic bacteria from food is critical to prevent epidemic food poisoning. However, the isolation of pathogenic bacteria from spoiled food is hampered by the lack of proper cell cultivation and/or isolation methods. Most of currently used methods suffer from complex, time-consuming culturing steps, low scalability, and high operation cost. Herein, we developed a ...
foodpathogens, etc ; Lactuca sativa var. capitata; O-antigens; Salmonella Typhimurium; ambient temperature; antibiotics; bacteriophages; biological control agents; cell viability; cucumbers; flagellum; fresh produce; genes; receptors; sequence analysis; Show all 15 Subjects
Abstract:
... Salmonella contamination of fresh produce is the primary bacterial cause of a significant number of foodborne outbreaks and infections. Bacteriophages can be used as natural antibacterial agents to control foodborne pathogens. However, the rapid development of bacterial resistance to phage infection is a significant barrier to practical phage application. To overcome this problem, we developed a n ...
foodpathogens, etc ; Aspergillus amstelodami; fungi; new species; Show all 4 Subjects
Abstract:
... Giving a correct name to a strain is an important step in biological research. A correct name is vital for optimal communication, and is often the link between various studies. Several names of commonly known food associated fungi changed in the last decade due to the changes in the nomenclatural rules, new insights in the classification and the description of new species. These changes might caus ...
Malcolm, Tan Turk Hsern; Chang, Wei San; Loo, Yuet Ying; Cheah, Yoke Kqueen; Radzi, Che Wan Jasimah Wan Mohamed; Kantilal, Haresh Kumar; Nishibuchi, Mitsuaki; Son, Radu
foodpathogens, etc ; Vibrio parahaemolyticus; clams; cleaning; cross contamination; decontamination; direct contact; food sanitation; hands; lettuce; slicing; Show all 11 Subjects
Abstract:
... Kitchen mishandling practices contribute to a large number of foodborne illnesses. In this study, the transfer and cross-contamination potential of Vibrio parahaemolyticus from bloody clams to ready-to-eat food (lettuce) was assessed. Three scenarios were investigated: 1) direct cross-contamination, the transfer of V. parahaemolyticus from bloody clams to non-food contact surfaces (hands and kitch ...
foodpathogens, etc ; Listeria monocytogenes; bacteria; quantitative polymerase chain reaction; skim milk; soft cheeses; yeast extract; Show all 7 Subjects
Abstract:
... Microplate immunocapture is an inexpensive method for the concentration of foodborne pathogens using an antibody-coated microplate. The objective of this study was to determine the efficacy of microplate immunocapture as an alternative to traditional enrichment for concentrating Listeria monocytogenes to levels detectable with selective plating or real-time PCR. L. monocytogenes isolates serologic ...
foodpathogens, etc ; color; detection limit; dielectric properties; energy; heat; microwave treatment; sugar content; water activity; Show all 9 Subjects
Abstract:
... The objective of this study was to investigate the effect of sugar content on dielectric properties of chili sauce, and inactivation efficiency when exposed to microwave energy. Heating history showed sugar content was an important factor influencing heating rate when chili sauce was exposed to microwave energy. Also, chili sauce of lower sugar content had a higher dielectric constant and dielectr ...
foodpathogens, etc ; epidemiological studies; essential genes; high-throughput nucleotide sequencing; molecular epidemiology; multilocus sequence typing; Show all 6 Subjects
Abstract:
... The technological advancement of molecular epidemiological analysis using next-generation sequencing (NGS) for foodborne pathogens has a groundbreaking impact over the past three years. In particular, the emergence of cg (core genome) multilocus sequence typing(MLST) has a significant impact. This is because this technology made it possible for many researchers to carry out molecular epidemiologic ...
... A paper chip device-based recombinase polymerase amplification (RPA) method was developed for highly sensitive and selective single-step detection of foodborne pathogens. A paper chip was manufactured by simply stacking functional papers. RPA reagents and fluorescent probe were dried on the reaction zone of a patterned poly(ether sulfone) membrane. The RPA reaction was initiated by adding pathogen ...
foodpathogens, etc ; Listeria monocytogenes; Raman spectroscopy; bacteria; benzalkonium chloride; cadmium; nanomaterials; principal component analysis; proteins; virulence; Show all 10 Subjects
Abstract:
... One of the potential applications of surface-enhanced Raman spectroscopy (SERS) is the detection of biological compounds and microorganisms. Here we demonstrate that SERS coupled with principal component analysis (PCA) serves as a perfect method for determining the taxonomic affiliation of bacteria at the strain level. We demonstrate for the first time that it is possible to distinguish different ...
foodpathogens, etc ; Escherichia coli O157; Listeria monocytogenes; Salmonella Typhimurium; cantaloupes; color; steam; surface roughness; watermelons; Show all 9 Subjects
Abstract:
... The purpose of this study was evaluation of the effectiveness of superheated steam (SHS) on inactivation of foodborne pathogens on cantaloupes and watermelons. Saturated steam (SS) treatment was performed at 100 °C and that of SHS at 150 and 200 °C. Escherichia coli O157:H7, Salmonella Typhimurium and Listeria monocytogenes-inoculated cantaloupes and watermelons were exposed for a maximum of 30 s ...
foodpathogens, etc ; Cronobacter; central nervous system; cross reaction; humans; hydrocephalus; infant formulas; infants; nanoparticles; nutrition; risk; Show all 11 Subjects
Abstract:
... Cronobacter species are foodborne pathogens that can affect the human central nervous system. Survivors of Cronobacter infections often suffer from severe neurological impairments, including hydrocephalus, quadriplegia, and developmental delays in all ages, especially in infants and the immunocompromised. Moreover, Cronobacter species pose a high risk in powdered infant formula (PIF) because PIF i ...
foodpathogens, etc ; analytical methods; gene amplification; loop-mediated isothermal amplification; nucleic acids; Show all 5 Subjects
Abstract:
... Isothermal amplification technology has developed rapidly in recent years. This form of nucleic acid amplification technology has the characteristics of high specificity, high sensitivity, convenience, and low cost. Isothermal amplification detection is expected to become an especially important method for the detection of foodborne and environmental pathogens. This review highlights the reaction ...
... Rapid detection of Listeria and other microbial pathogens in food is an essential part of quality control and it is critical for ensuring the safety of consumers. Culture-based methods for detecting foodborne pathogens are time-consuming, laborious and cannot detect viable but non-culturable microorganism, whereas viability PCR methodology provides quick results; it is able to detect viable but no ...
foodpathogens, etc ; Escherichia coli O157; Listeria monocytogenes; Salmonella enterica; bags; polyethylene; polystyrenes; table grapes; temperature; Show all 9 Subjects
Abstract:
... We examined the survival of Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella enterica Thompson inoculated on commercially packed table grapes under simulated refrigerated transit conditions (1.1 ± 0.5 °C; 90% RH). Grapes were placed in perforated polyethylene cluster bags, within a commercial expanded polystyrene box equipped with either a SO2-generating pad; a perforated polyethyl ...
foodpathogens, etc ; Staphylococcus aureus; bacteria; biosensors; electrochemistry; food safety; foodborne illness; public health; Show all 8 Subjects
Abstract:
... Foodborne illness outbreaks caused by the consumption of food contaminated with harmful bacteria has drastically increased in the past decades. Therefore, detection of harmful bacteria in the food has become an important factor for the recognition and prevention of problems associated with food safety and public health. Staphylococcus aureus is one of the most commonly isolated foodborne pathogen ...
foodpathogens, etc ; Vibrio parahaemolyticus; antibiotics; bacteriophages; coasts; genes; genomics; metabolism; phylogeny; Korean Peninsula; Show all 10 Subjects
Abstract:
... Vibrio parahaemolyticus, a foodborne pathogen, has become resistant to antibiotics. Therefore, alternative bio-control agents such bacteriophage are urgently needed for its control. Six novel bacteriophages specific to V. parahaemolyticus (vB_VpaP_KF1~2, vB_VpaS_KF3~6) were characterized at the molecular level in this study. Genomic similarity analysis revealed that these six bacteriophages could ...
foodpathogens, etc ; Cronobacter sakazakii; antibodies; detection limit; immunoassays; infant formulas; nanogold; neonates; Show all 8 Subjects
Abstract:
... Cronobacter sakazakii is an opportunistic foodborne pathogen that can infect newborns through powdered infant formula (PIF). In this study, we developed a novel enhanced lateral flow immunoassay (LFA) with enhanced sensitivity for detection of C. sakazakii in PIF by the naked eye. The proposed strategy for signal enhancement of the traditional LFA used concentrated gold nanoparticles (AuNP) as the ...
foodpathogens, etc ; DNA; Listeria monocytogenes; food contamination; genes; genomics; milk; morbidity; polymerase chain reaction; serotypes; Show all 10 Subjects
Abstract:
... Listeria monocytogenes serotype 1/2a is considered as a foodborne pathogen, resulting in high morbidity. Detection of Listeria monocytogenes serotype 1/2a is rather difficult and time-consuming thus, the need for better detection methods is encouraged. In order to rapidly detect and identify L. monocytogenes serotype 1/2a, specific target genes were mined by the comparative genomics approach. Thre ...