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1. Protease inhibitor from Libidibia ferrea seeds attenuates inflammatory and nociceptive responses in mice

Author:
Raquel Carvalho; Iluska Senna Bonfá; Jéssica de Araújo Isaías Muller; Silvana Cristina Pando; Mônica Cristina Toffoli-Kadri
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115694
ISSN:
0378-8741
Subject:
formalin; formalin test,   etc   ; Caesalpinia ferrea; acetic acid; adhesion; analgesic effect; anti-inflammatory activity; asthma; bark; bronchitis; cell adhesion; cytokines; cytotoxicity; edema; enterocolitis; fever; fruits; hydrogen peroxide; leukocytes; macrophages; males; mast cells; nitric oxide; pain; permeability; pharyngitis; proteinase inhibitors; proteinases; rheumatoid arthritis; traditional medicine; trees;     Show all 31 Subjects
Abstract:
... Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz is a Brazilian native tree locally known as jucá and pau-ferro, and it has been used in folk medicine for relieving, asthma, bronchitis, sore throat, rheumatism, enterocolitis and fever. The anti-inflammatory properties of L. ferrea were confirmed for its stem, fruit, leaves, bark and seeds extracts, however little is known about the natural compounds ...
DOI:
10.1016/j.jep.2022.115694

2. Analgesic, anti-inflammatory activity and metabolite profiling of the methanolic extract of Callicarpa arborea Roxb. leaves

Author:
Rubaet Sharmin Ema; S.M. Neamul Kabir Zihad; Md Naharul Islam; Nazifa Sifat; Razina Rouf; Jamil A. Shilpi; Shaikh Jamal Uddin
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115757
ISSN:
0378-8741
Subject:
formalin test,   etc   ; Callicarpa; acetic acid; acute toxicity; albino; analgesic effect; analgesics; anti-inflammatory activity; chemical composition; dose response; edema; egg albumen; gas chromatography-mass spectrometry; hot plate test; metabolites; mortality; pain; phytochemicals; rheumatoid arthritis; toxicity testing; traditional medicine; tribal peoples; Bangladesh; India;     Show all 24 Subjects
Abstract:
... Callicarpa arborea Roxb. is widely used as traditional medicine especially by the tribal people of Bangladesh in the management of wide range of ailments. In addition to Bangladesh, the leaves of this plant is utilized as a remedy to various painful and inflammatory conditions including rheumatism, toothache and stomachache in other countries of Indian subcontinent. Depending on the ethnomedicinal ...
DOI:
10.1016/j.jep.2022.115757

3. Orexin receptors in the CA1 region of hippocampus modulate the stress-induced antinociceptive responses in an animal model of persistent inflammatory pain

Author:
Fatemeh Zareie; Seyedehdelaram Ghalebandi; Kobra Askari; Zahra Mousavi; Abbas Haghparast
Source:
Peptides 2022 v.147 pp. 170679
ISSN:
0196-9781
Subject:
formalin; formalin test,   etc   ; adults; analgesia; analgesic effect; animal models; antagonists; hippocampus; hypothalamus; males; neurotransmitters; pain; peptides;     Show all 13 Subjects
Abstract:
... Stress activates multiple neural pathways and neurotransmitters that often suppress pain perception, the phenomenon called stress-induced analgesia (SIA). Orexin neurons from the lateral hypothalamus project to entire brain structures such as the hippocampus. The present study examined this hypothesis that orexinergic receptors in the CA1 region of the hippocampus may play a modulatory role in the ...
DOI:
10.1016/j.peptides.2021.170679

4. Orbitacolax kukiensis sp. nov. (Copepoda, Cyclopoida, Bomolochidae) recovered from an uncertain host in Japan

Author:
Kunihiko Izawa
Source:
Crustaceana 2022 v.95 no.3 pp. 325-330
ISSN:
0011-216X
Subject:
formalin,   etc   ; Actinopterygii; Cyclopoida; Japan; females;     Show all 5 Subjects
Abstract:
... Orbitacolax kukiensis sp. nov. is described based on specimens of the female recovered from a solution of formalin in which eight species of coastal actinopterygian fishes had been fixed and preserved all together. These fishes had earlier been captured by means of a fixed net at Kuki, Mie Prefecture, Japan. ...
DOI:
10.1163/15685403-bja10186

5. The antinociceptive effect of manual acupuncture in the auricular branch of the vagus nerve in visceral and somatic acute pain models and its laterality dependence

Author:
Marcos Lisboa Neves; Jhenifer Karvat; Róli Rodrigues Simões; Guilherme Fleury Fina Speretta; Renata Maria Lataro; Morgana Duarte da Silva; Adair Roberto Soares Santos
Source:
Life sciences 2022 pp. 121000
ISSN:
0024-3205
Subject:
formalin; formalin test,   etc   ; acetic acid; acupuncture; analgesic effect; brain; females; ganglia; males; models; nociception; pain; vagus nerve;     Show all 13 Subjects
Abstract:
... The vagus nerve provides an important route to the central nervous system, and its brain projections are involved in nociceptive control and pain perception. We investigated the effect of ABVN stimulation on the inhibition of nociceptive signaling and the role of the cholinergic system in its neurobiological effects in models of visceral-somatic pain in rats, as well as the potential difference in ...
DOI:
10.1016/j.lfs.2022.121000

6. Atmospheric-Pressure Infrared Laser-Ablation Plasma-Postionization Mass Spectrometry Imaging of Formalin-Fixed Paraffin-Embedded (FFPE) and Fresh-Frozen Tissue Sections with No Sample Preparation

Author:
Rory T. Steven; Marcel Niehaus; Adam J. Taylor; Ammar Nasif; Efstathios Elia; Richard J. A. Goodwin; Zoltan Takats; Josephine Bunch
Source:
Analytical chemistry 2022 v.94 no.28 pp. 9970-9974
ISSN:
1520-6882
Subject:
formalin,   etc   ; analytical chemistry; atmospheric pressure; lipids; metabolites; motivation;     Show all 6 Subjects
Abstract:
... Mass spectrometry imaging (MSI) encompasses a powerful suit of techniques which provide spatially resolved atomic and molecular information from almost any sample type. MSI is now widely used in preclinical research to provide insight into metabolic phenotypes of disease. Typically, fresh-frozen tissue preparations are considered optimal for biological MSI and other traditional preservation method ...
DOI:
10.1021/acs.analchem.2c00690

7. Nothobomolochus aigo sp. nov. (Copepoda, Cyclopoida, Bomolochidae) parasitic on the mottled spinefoot Siganus fuscescens (Houttuyn, 1782) (Pisces, Acanthuriformes, Siganidae) in Japan

Author:
Kunihiko Izawa
Source:
Crustaceana 2022 v.95 no.3 pp. 345-351
ISSN:
0011-216X
Subject:
formalin,   etc   ; Acanthuriformes; Cyclopoida; Japan; Siganus; females; fish;     Show all 7 Subjects
Abstract:
... Nothobomolochua aigo sp. nov. is described based on two specimens of the female recovered from the bucco-branchial cavity of the mottled spinefoot, Siganus fuscescens (Houttuyn, 1782), captured by a fixed net at Kuki, Mie Prefecture. The fish was subsequently found in a vessel with formalin solution in which eight species of coastal actinopterygian fishes, including S. fuscescens, all captured by ...
DOI:
10.1163/15685403-bja10192

8. Investigating the anti-inflammatory and analgesic properties of leaves ethanolic extracts of Cedruslibani and Pinusbrutia

Author:
Lina Karrat; Mohammad Yaser Abajy; Ream Nayal
Source:
Heliyon 2022 v.8 no.4 pp. e09254
ISSN:
2405-8440
Subject:
formalin; formalin test,   etc   ; albumins; analgesic effect; analgesics; anti-inflammatory activity; denaturation; diclofenac; gels; hemolysis; humans; lidocaine; plant extracts; tail; tail flick test;     Show all 15 Subjects
Abstract:
... This investigation aimed to assess the anti-inflammatory and analgesic effects of Cedruslibani and Pinusbrutia leaves. The anti-inflammatory property was evaluated by Human Red Blood Cells (HRBC) membrane stabilization assay and Albumin denaturation assay using Sodium diclofenac as a positive control. To evaluate the analgesic property, formalin and tail flick tests were carried out using ethanoli ...
DOI:
10.1016/j.heliyon.2022.e09254

9. Deep oncopanel sequencing reveals within block position-dependent quality degradation in FFPE processed samples

Author:
Yifan Zhang; Thomas M. Blomquist; Rebecca Kusko; Daniel Stetson; Zhihong Zhang; Lihui Yin; Robert Sebra; Binsheng Gong; Jennifer S. Lococo; Vinay K. Mittal; Natalia Novoradovskaya; Ji-Youn Yeo; Nicole Dominiak; Jennifer Hipp; Amelia Raymond; Fujun Qiu; Hanane Arib; Melissa L. Smith; Jay E. Brock; Daniel H. Farkas; Daniel J. Craig; Erin L. Crawford; Dan Li; Tom Morrison; Nikola Tom; Wenzhong Xiao; Mary Yang; Christopher E. Mason; Todd A. Richmond; Wendell Jones; Donald J. Johann Jr; Leming Shi; Weida Tong; James C. Willey; Joshua Xu
Source:
Genome biology 2022 v.23 no.1 pp. 141
ISSN:
1474-760X
Subject:
formalin,   etc   ; DNA; cell lines; diploidy; genome; genomics; histology; mutation; patients; therapeutics;     Show all 10 Subjects
Abstract:
... BACKGROUND: Clinical laboratories routinely use formalin-fixed paraffin-embedded (FFPE) tissue or cell block cytology samples in oncology panel sequencing to identify mutations that can predict patient response to targeted therapy. To understand the technical error due to FFPE processing, a robustly characterized diploid cell line was used to create FFPE samples with four different pre-tissue proc ...
DOI:
10.1186/s13059-022-02709-8

10. Evaluation of the treatment of fever, pain and inflammation with Indigofera suffruticosa Miller Leaves Aqueous Extract

Author:
Weber Melo Nascimento; João Ricardhis Saturnino Oliveira; Rebeca Xavier Cunha; Daywison Silva Rodrigues Gambôa; Ana Paula Sant'Anna Silva; Vera Lúcia de Menezes Lima
Source:
Journal of ethnopharmacology 2022 v.287 pp. 114958
ISSN:
0378-8741
Subject:
formalin; formalin test,   etc   ; Indigofera suffruticosa; acute toxicity; anti-inflammatory activity; edema; fever; indomethacin; inflammation; leukocytes; nitric oxide; pain; peritonitis; protein denaturation; quercetin; thin layer chromatography; traditional medicine;     Show all 17 Subjects
Abstract:
... Indigofera suffruticosa has reports of its popular use for analgesy in several cultures. Besides that, all parts of this plant are used for some medicinal outcome. The leaves are used in teas, decoctions, juices and included in baths for treating fever and inflammatory processes.To evaluate the anti-inflammatory activity mechanisms of I. suffruticosa leave aqueous extract (IsAE).Phytochemical scre ...
DOI:
10.1016/j.jep.2021.114958

11. Adapted lead (Pb) histochemical detection procedure in tissue samples of blue crabs Callinectes danae (Smith, 1869)

Author:
André Luiz Veiga Conrado; Renata Stecca Iunes; Matheus Santos Costa; Giovana da Silva Chagas; Rebecca Castro; Denis Moledo de Souza Abessa; José Roberto Machado Cunha da Silva; Isabella Cristina Bordon
Source:
Journal of trace elements and minerals 2022 pp. 100022
ISSN:
2773-0506
Subject:
formalin,   etc   ; Callinectes danae; crabs; hepatopancreas; histochemistry; lead; methylene blue; potassium; wildlife;     Show all 9 Subjects
Abstract:
... Lead (Pb) cellular deposits can be detected by histochemistry using a classical potassium dichromate - formalin fixation and post-fixation with 3% potassium dichromate solution. Here we describe an alternative procedure for Pb histochemical detection in the blue crab Callinectes danae. Animals were exposed to Pb (2 µg/mL) for 24, 48 and 96h. Then, buffered formalin solution was used for hepatopanc ...
DOI:
10.1016/j.jtemin.2022.100022

12. 2,4-dinitrophenylhydrazine capturing combined with mass defect filtering strategy to identify aliphatic aldehydes in biological samples

Author:
Si-Yu Wang; Hui Liu; Jin-Hao Zhu; Shan-Shan Zhou; Jin-Di Xu; Jing Zhou; Qian Mao; Ming Kong; Song-Lin Li; He Zhu
Source:
Journal of chromatography 2022 v.1679 pp. 463405
ISSN:
0021-9673
Subject:
formalin,   etc   ; blood serum; immunosuppression; oxidative stress; toxicity; ultra-performance liquid chromatography;     Show all 6 Subjects
Abstract:
... Aliphatic aldehydes are toxic substances that correlate with the onset of many diseases. However, up to date, the methods to identify aliphatic aldehydes in biological samples are less selectivity and/or robustness. In this study, a strategy based on 2,4-dinitrophenylhydrazine (DNPH) capturing combined with mass defect filtering (MDF) was established and validated to identify aliphatic aldehydes i ...
DOI:
10.1016/j.chroma.2022.463405

13. Length–weight relationship of eight native fish species from Paraguaçu River basin, Brazil

Author:
Fabiane Barreto Souza; Alexandre Clistenes de Alcantara Santos; Nuno Caiola
Source:
Journal of applied ichthyology 2022 v.38 no.3 pp. 336-339
ISSN:
0175-8659
Subject:
formalin,   etc   ; Geophagus; allometry; freshwater fish; ichthyology; indigenous species; regression analysis; rivers; watersheds; Brazil;     Show all 10 Subjects
Abstract:
... This study reports the first record of Length‐Weight Relationships (LWR) of eight endemic freshwater fish species from the Paraguaçu River basin, Northeast Brazil. Sampling was conducted in thirteen streams using electrofishing. Only Kalyptodoras bahiensis specimens from the middle section of Paraguaçu river were obtained from local fishermen. Fishes were fixed with 4% formalin before measurement, ...
DOI:
10.1111/jai.14308

14. Insight into the possible mechanism(s) involved in the antinociceptive and antineuropathic activity of Descurainia sophia L. Webb ex Prantl essential oil

Author:
Donya Ziafatdoost Abed; Sajjad Jabbari; Zainul Amiruddin Zakaria; Saeed Mohammadi
Source:
Journal of ethnopharmacology 2022 v.298 pp. 115638
ISSN:
0378-8741
Subject:
formalin; formalin test,   etc   ; Descurainia sophia; analgesic effect; cannabinoids; dopamine; essential oils; fever; glibenclamide; headache; indigenous species; inflammation; linolenic acid; methylene blue; models; serotonin; somatosensory disorders; spinal cord; traditional medicine; Iran;     Show all 20 Subjects
Abstract:
... Descurainia sophia (L.)(Brassicaceae), popularly known as “Khaksheer”, is a native species widely distributed in Iran. The seeds and essential oil has been used in local traditional medicine (Persian folk ethnomedicine) to treat fever, inflammation, back pain, and headache. To investigate in vitro anti-nociceptive and antineuropathic activities of Descurainia sophia seeds essential oil (DSEO) in r ...
DOI:
10.1016/j.jep.2022.115638

15. Histological, histochemical, ultrastructural and immunohistochemical identification and characterization of the neurosecretory cells of the adult rabbit's adrenal medulla

Author:
Usama Kamal Moawad; Shehata Mohamed Mohamed Soliman; Khaled Mohamed Mazher; Randa Mohamed Hassan; Taghreed Mohamed Nabil
Source:
Anatomia, histologia, embryologia 2022 v.51 no.2 pp. 280-288
ISSN:
0340-2096
Subject:
formalin,   etc   ; adrenal medulla; adults; alum; antibodies; color; epinephrine; immunohistochemistry; norepinephrine; rabbits; silver; New Zealand;     Show all 12 Subjects
Abstract:
... The present investigation was conducted on the adrenal glands of 40 adult New Zealand rabbits of both sexes to characterize and identify the histological, histochemical, ultrastructural, and immunohistochemical features of the neurosecretory cells of the adrenal medulla. The obtained specimens of adrenal medulla were subjected to routine histological techniques and then stained with different hist ...
DOI:
10.1111/ahe.12789

16. Surgical excision and differential histopathological attributes of vaginal fibroma in a canine female: A pathomorphological study

Author:
Deeksha Bharti; Akshay Kumar; Shiv Kumar; Dhaleshwari; Dipanshu Bisht; Manjusha; Abhishek Bhardwaj; Rajneesh; Manish Arya
Source:
Indian journal of veterinary pathology 2022 v.46 no.1 pp. 90-92
ISSN:
0973-970X
Subject:
formalin,   etc   ; animal pathology; case studies; collagen; crossbreds; dogs; estrus; excision; females; fibroblasts; fibroma; histopathology;     Show all 12 Subjects
Abstract:
... Fibromas are usually benign, hard, white, spherical and are mesenchymal neoplasm of fibroblasts with abundant collagenous stroma. This case report illustrates a female dog with vaginal fibroma condition. A 6-year-old intact nulliparous, crossbreed female dog was presented with the history of vulvar bleeding and tenesmus. The dog had regular oestrus cycles, but was incompetent in conceiving. Vagina ...
DOI:
10.5958/0973-970X.2022.00014.1

17. Impact of standardization in tissue processing: the performance of different fixatives

Author:
Eleonora De Martino; Caterina Medeot; Lorenzo D’Amico; Giorgio Stanta; Serena Bonin
Source:
New biotechnology 2022 v.71 pp. 30-36
ISSN:
1871-6784
Subject:
formalin,   etc   ; DNA; RNA; ambient temperature; biotechnology; histology; liver; mice; micro-computed tomography; refrigeration;     Show all 10 Subjects
Abstract:
... Most tissues in clinical practice are formalin-fixed and paraffin-embedded for histological as well as molecular analyses. The reproducibility and uniformity of molecular analyses is strictly dependent on the quality of the biomolecules, which is highly influenced by pre-analytical processes. In this study, the effect of different fixatives was compared, including formalin, Bouin’s solution, RCL2® ...
DOI:
10.1016/j.nbt.2022.07.001

18. Anti-inflammatory, antinociceptive effects and involvement of opioid receptors in the antinociceptive activity of Eugenia uniflora leaves obtained with water, ethanol, and propylene glycol mixture

Author:
Glenda Laíssa Oliveira de Melo Candeia; Wêndeo Kennedy Costa; Alisson Macário de Oliveira; Thiago Henrique Napoleão; Patrícia Maria Guedes Paiva; Magda Rhayanny Assunção Ferreira; Luiz Alberto Lira Soares
Source:
Journal of ethnopharmacology 2022 v.296 pp. 115508
ISSN:
0378-8741
Subject:
formalin; formalin test,   etc   ; Eugenia uniflora; analgesic effect; analgesics; anti-inflammatory activity; edema; ellagic acid; ethanol; ethyl acetate; flavonoids; gallic acid; indigenous species; inflammation; naloxone; neutrophils; peritonitis; propylene glycol; spray drying; tail flick test; traditional medicine; Brazil;     Show all 22 Subjects
Abstract:
... Eugenia uniflora (Myrtaceae) is a species native to Brazil and has a traditional use in the treatment of inflammation. To evaluate the anti-inflammatory and antinociceptive effects, and the involvement of opioid receptors in the antinociceptive activity of extract and fractions from Eugenia uniflora leaves. TLC and HPLC were used to characterize the spray-dried extract (SDE) and fractions. In the ...
DOI:
10.1016/j.jep.2022.115508

19. Aerosol Survival, Disinfection and Formalin Inactivation of Nipah Virus

Author:
Sophie J. Smither; Lin S. Eastaugh; Lyn M. O’Brien; Amanda L. Phelps; Mark S. Lever
Source:
Viruses 2022 v.14 no.9 pp. -
ISSN:
1999-4915
Subject:
formalin,   etc   ; Nipah henipavirus; aerosols; animals; culture media; death; disinfection; ethanol; sodium hypochlorite; tissue culture; viruses;     Show all 11 Subjects
Abstract:
... Nipah virus is a relatively newly discovered emerging virus on the WHO list of priority pathogens which has the potential to cause outbreaks with high fatality rates. Whilst progress is being made in the development of animal models for evaluating vaccines and therapies, some of the more fundamental data on Nipah virus are lacking. We performed studies to generate novel information on the aerosol ...
DOI:
10.3390/v14092057

20. A Stereological Study of the Three Types of Ganglia of Male, Female, and Undifferentiated Scrobicularia plana (Bivalvia)

Author:
Sukanlaya Tantiwisawaruji; Maria J. Rocha; Ana Silva; Miguel A. Pardal; Uthaiwan Kovitvadhi; Eduardo Rocha
Source:
Animals 2022 v.12 no.17 pp. -
ISSN:
2076-2615
Subject:
formalin,   etc   ; Scrobicularia plana; adults; cortex; females; ganglia; gonads; light microscopy; males; neuroglia; neurons; neurophysiology; neurotransmitters;     Show all 13 Subjects
Abstract:
... Neurotransmitters modulate gonadal maturation in bivalves. However, it remains unclear whether there are differences in the nervous system structure between sexes, maturation, and ganglia. Therefore, a stereological study was conducted on the ganglia of adult peppery furrow shell (Scrobicularia plana). Equal-sized males, females, and undifferentiated (gamete absence) animals were fixed with 10% fo ...
DOI:
10.3390/ani12172248