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1. The Escherichia coli Amino Acid Uptake Protein CycA: Regulation of Its Synthesis and Practical Application in l-Isoleucine Production

Author:
Christine Hook; Natalya Eremina; Petr Zaytsev; Daria Varlamova; Nataliya Stoynova
Source:
Microorganisms 2022 v.10 no.3 pp. -
ISSN:
2076-2607
Subject:
gel electrophoresis,   etc   ; Escherichia coli; reporter genes;     Show all 3 Subjects
Abstract:
... Amino acid transport systems perform important physiological functions; their role should certainly be considered in microbial production of amino acids. Typically, in the context of metabolic engineering, efforts are focused on the search for and application of specific amino acid efflux pumps. However, in addition, importers can also be used to improve the industrial process as a whole. In this ...
DOI:
10.3390/microorganisms10030647

2. Characterization of the Reduced IgE Binding Capacity in Boiled and Autoclaved Soybeans through Proteomic Approaches

Author:
Xiaowen Pi; Yuxue Sun; Xiaomin Deng; Dawei Xin; Jianjun Cheng; Mingruo Guo
Source:
Foods 2022 v.11 no.3 pp. -
ISSN:
2304-8158
Subject:
polyacrylamide gel electrophoresis,   etc   ; allergens; autoclaving; epitopes; heat; peptides; proteomics; soybeans;     Show all 8 Subjects
Abstract:
... The study investigated the changes in IgE binding capacity, protein profiles and peptide compositions after soybeans were boiled and autoclaved. The results of ELISA showed that the IgE binding capacity of soybean was reduced by 69.3% and 88.9% after boiling and autoclaving, respectively. Above 43 and 10 kDa proteins disappeared in boiled and autoclaved soybeans from SDS-PAGE, respectively. A Venn ...
DOI:
10.3390/foods11030479

3. Faster and sensitive zymographic detection of oxidases generating hydrogen peroxide. The case of diamine oxidase

Author:
Paul Chomdom Kounga; Armelle Tchoumi Neree; Paola Pietrangeli; Lucia Marcocci; Mircea Alexandru Mateescu
Source:
Analytical biochemistry 2022 v.648 pp. 114676
ISSN:
0003-2697
Subject:
polyacrylamide gel electrophoresis,   etc   ; color; diamine oxidase; hydrogen peroxide; peroxidase; stabilizers;     Show all 6 Subjects
Abstract:
... The existing zymography method for the detection of diamine oxidase (DAO) activity has been improved by a new staining procedure with the aim to ameliorate its sensitivity. Both procedures used SDS-PAGE gels containing uniformly distributed entrapped peroxidase (that wouldn't migrate during electrophoresis). The new approach with 3,5-dichloro-2-hydroxybenzenesulfonate (DCHBS) as peroxidase substra ...
DOI:
10.1016/j.ab.2022.114676

4. Colloidal dynamics of emulsion droplets in mouth

Author:
Ivanna Colijn; Anthony Ash; Marie Dufauret; Melissa lepage; Céline Loussert-Fonta; Martin E. Leser; Peter J. Wilde; Tim J. Wooster
Source:
Journal of colloid and interface science 2022 v.620 pp. 153-167
ISSN:
0021-9797
Subject:
polyacrylamide gel electrophoresis,   etc   ; astringency; bioadhesives; emulsions; fluorescence; hydrophobicity; mucins; mucoadhesion; saliva; tongue;     Show all 10 Subjects
Abstract:
... The interaction of emulsions with the tongue is key to the sensory appeal of food and can potentially be exploited for oral/buccal pharmaceutical delivery. Whilst there is good understanding of the different mucoadhesive forces governing emulsion interaction with the tongue, their relative importance is not well understood. In addition, the physical location of emulsions within the saliva papillae ...
DOI:
10.1016/j.jcis.2022.03.117

5. Functional properties of peptides obtained from whey proteins by ficin extract hydrolysis

Author:
Afaf Kheroufi; María E. Brassesco; Débora A. Campos; Halima Boughellout; Manuela E. Pintado
Source:
Food bioscience 2022 v.47 pp. 101707
ISSN:
2212-4292
Subject:
polyacrylamide gel electrophoresis,   etc   ; antioxidant activity; ficain; hydrolysis; liquid chromatography; peptides; solubility; whey;     Show all 8 Subjects
Abstract:
... Enzymatic whey proteins concentrates (WPC) hydrolysis under optimal conditions of ficin crude extract (FCE) in two ratios E/S (0.5 and 1%) was studied, assessing the impact of hydrolysis on WPC by determination degree of hydrolysis (DH), Fast proteins liquid chromatography (FPLC), Tricine SDS-PAGE, antioxidant activity at sampling time beside, and technological properties (solubility, emulsifying ...
DOI:
10.1016/j.fbio.2022.101707

6. Separation methods for milk proteins on polyacrylamide gel electrophoresis: Critical analysis and options for better resolution

Author:
Neelima Sharma; Rajan Sharma; Yudhishthir S. Rajput; Bimlesh Mann; Richa Singh; Kamal Gandhi
Source:
International dairy journal 2021 v.114 pp. 104920
ISSN:
0958-6946
Subject:
polyacrylamide gel electrophoresis; two-dimensional gel electrophoresis,   etc   ; milk; sulfates; whey;     Show all 5 Subjects
Abstract:
... Over the years, a number of methodological variations in polyacrylamide gel electrophoresis (PAGE) have been attempted for the separation of milk proteins. Caseins are similar in size and differ very little in net charge from each other. Also, in heated milk, κ-casein not only complexes with whey proteins but also undergoes self-polymerisation. These cause problems in the resolution of milk protei ...
DOI:
10.1016/j.idairyj.2020.104920

7. Einsatz von Proteasen zur Verbesserung der Filtration und Stabilität des österreichischen Weines

Author:
Karin Mandl; Jasmina Suljic; Harald Scheiblhofer; Michael Winkler; Michael Schneider
Source:
Die Bodenkultur 2022 v.72 no.2 pp. 93-103
ISSN:
2719-5430
Subject:
polyacrylamide gel electrophoresis,   etc   ; aspergillopepsin; bentonite; disease prevention; enzymatic treatment; filterability; filtration; taste; wines;     Show all 9 Subjects
Abstract:
... Wine haze often occurs after bottling due to insufficient protein stabilization and is often referred as a quality defect by customers. To prevent wine haze, bentonite is commonly used to remove certain protein fractions. Proteases may represent an alternative to bentonite, especially Aspergillopepsin. The aim of this work was to find out if there are advantages for winemaker by using an acid prot ...
DOI:
10.2478/boku-2021-0009

8. Strain‐dependent effectivity, and protective role against enzymes of S‐layers in Lactiplantibacillus plantarum strains

Author:
Duygu Alp
Source:
Journal of basic microbiology 2022 v.62 no.5 pp. 555-567
ISSN:
0233-111X
Subject:
polyacrylamide gel electrophoresis,   etc   ; adhesion; gastrointestinal system; lithium chloride; lysozyme; phenol; protective effect; viability;     Show all 8 Subjects
Abstract:
... The present study investigated whether the surface layer (S‐layer), which is known to have a varying effect from strain to strain on aggregation, adhesion ability, also has an effect on the resistance of bacteria to digestive enzymes, phenol, lysozymes. The effect of S‐layers on the resistance against various enzymes, aggregation and adhesion abilities, and strain specificity were determined of ei ...
DOI:
10.1002/jobm.202100590

9. A novel G·G·T non-conventional intramolecular triplex formed by the double repeat sequence of Chlamydomonas telomeric DNA [Erratum: June 2022, v.12(27); p.170496]

Author:
Aparna Bansal; Priyanka Phogat; Shrikant Kukreti
Source:
RSC advances 2022 v.12 no.25 pp. 15918-15924
ISSN:
2046-2069
Subject:
gel electrophoresis,   etc   ; Chlamydomonas reinhardtii; DNA; circular dichroism spectroscopy; fluorescence; nuclear magnetic resonance spectroscopy; telomeres;     Show all 7 Subjects
Abstract:
... Competition among DNA non-canonical structures has been widely studied in repetitive DNA sequences. The factors affecting DNA structural polymorphism have always been an important area of research. The Chlamydomonas reinhardtii telomere (TTTTAGGG)ₙ is found to be an exception to the general idea of forming a folded G-quadruplex by a few repeats in any telomeric sequence. Herein, using gel electrop ...
DOI:
10.1039/d2ra00861k

10. Characterization of prolamin recycled from the byproduct of the Baijiu brewing industry (Jiuzao) by SDS-PAGE, multispectral analysis, and morphological analysis

Author:
Lin Zhu; Xing Li; Xuebo Song; Qing Li; Fuping Zheng; Hehe Li; Jinyuan Sun; Mingquan Huang; Baoguo Sun
Source:
Food bioscience 2022 pp. 101854
ISSN:
2212-4292
Subject:
polyacrylamide gel electrophoresis,   etc   ; active ingredients; byproducts; encapsulation; fermentation; hydrophobicity; kafirins; multispectral imagery; prolamins; solubility;     Show all 10 Subjects
Abstract:
... Prolamins have attracted increasing attention in recent years because of their potential application in active ingredient encapsulation and food packaging. As a byproduct of the Baijiu brewing industry with huge production (40 million tons every year), Jiuzao is a great source of prolamin because the fermentation process consumes mainly starch instead of protein. However, information on the extrac ...
DOI:
10.1016/j.fbio.2022.101854

11. Complement C1q in plasma induces nonspecific binding of poly(acrylic acid)-coated upconverting nanoparticle antibody conjugates

Author:
Saara Kuusinen; Miikka Ekman; Kirsti Raiko; Heidi Hannula; Annika Lyytikäinen; Satu Lahtinen; Tero Soukka
Source:
Analytical and bioanalytical chemistry 2022 v.414 no.12 pp. 3741-3749
ISSN:
1618-2642
Subject:
gel electrophoresis,   etc   ; analytical chemistry; antibodies; chromatography; complement; immunoassays; ionic strength; mass spectrometry; troponin I;     Show all 9 Subjects
Abstract:
... Upconverting nanoparticles are attractive reporters for immunoassays, because their high specific activity and lack of autofluorescence background enable their detection at extremely low concentrations. However, the sensitivity achieved with heterogeneous sandwich immunoassays using nanoparticle reporters is generally limited by the nonspecific binding of nanoparticle antibody conjugates to solid ...
DOI:
10.1007/s00216-022-04021-7

12. One-step extraction of oat protein by choline chloride-alcohol deep eutectic solvents: Role of chain length of dihydric alcohol

Author:
Jianxiong Yue; Zhenbao Zhu; Jianhua Yi; Hui Li; Bingcan Chen; Jiajia Rao
Source:
Food chemistry 2022 v.376 pp. 131943
ISSN:
0308-8146
Subject:
polyacrylamide gel electrophoresis,   etc   ; alcohols; biorefining; choline; choline chloride; food chemistry; oat protein; oats; pH;     Show all 9 Subjects
Abstract:
... The aim of this study is to investigate the effect of dihydric alcohol chain length (1,2-ethanediol, 1,3-propanediol, and 1,4-butanediol) on the structure of deep eutectic solvents (DESs) and the properties of the extracted oat proteins. Herein, five anhydrous and nine hydrated DESs were successfully prepared by mixing choline chloride, dihydric alcohol, and/or water in a heating method. The struc ...
DOI:
10.1016/j.foodchem.2021.131943

13. Stronger together: Analytical techniques for recombinant adeno associated virus

Author:
Andrei Hutanu; David Boelsterli; Claudio Schmidli; Cristina Montealegre; Mike H. N. Dang Thai; Balazs Bobaly; Marius Koch; Maria A. Schwarz
Source:
Electrophoresis 2022 v.43 no.9-10 pp. 1107-1117
ISSN:
0173-0835
Subject:
gel electrophoresis,   etc   ; DNA; Dependoparvovirus; capillary zone electrophoresis; quality control; transgenes; ultracentrifugation; viral genome; viruses;     Show all 9 Subjects
Abstract:
... With recent FDA approval of two recombinant adeno‐associated virus (rAAV)‐based gene therapies, these vectors have proven that they are suitable to address monogenic diseases. However, rAAVs are relatively new modalities, and their production and therapy costs significantly exceed those of conventional biologics. Thus, significant efforts are made to improve the processes, methods, and techniques ...
DOI:
10.1002/elps.202100302

14. Venom of several Indian green pit vipers: Comparison of biochemical activities and cross-reactivity with antivenoms

Author:
Susmita Thakur; Anita Malhotra; Surajit Giri; H.T. Lalremsanga; Omesh K. Bharti; Vishal Santra; Gerard Martin; Robin Doley
Source:
Toxicon 2022 v.210 pp. 66-77
ISSN:
0041-0101
Subject:
polyacrylamide gel electrophoresis,   etc   ; Trimeresurus; antivenoms; coagulation; community health; cross reaction; hemolysis; phospholipases; venoms; India;     Show all 10 Subjects
Abstract:
... Green pit vipers, a name that can refer to several unrelated species, comprise a large group of venomous snakes found across the humid areas of tropical and sub-tropical Asia, and are responsible for most of the bite cases across this region. In India, green pit vipers belonging to several genera are prevalent in the northern and north-eastern hilly region, unrelated to species present in the peni ...
DOI:
10.1016/j.toxicon.2022.02.014

15. A novel ribosome-dimerization protein found in the hyperthermophilic archaeon Pyrococcus furiosus using ribosome-associated proteomics

Author:
Chiaki Yaeshima; Natsumi Murata; Sonoko Ishino; Ikuko Sagawa; Kosuke Ito; Toshio Uchiumi
Source:
Biochemical and biophysical research communications 2022 v.593 pp. 116-121
ISSN:
0006-291X
Subject:
gel electrophoresis,   etc   ; Pyrococcus furiosus; dimerization; liquid chromatography; protein synthesis; proteomics; research; ribosomes; tandem mass spectrometry;     Show all 9 Subjects
Abstract:
... Ribosome dimerization is one of the bacterial events that suppresses protein synthesis in the stationary phase. Protein factors responsible for ribosome dimerization in bacteria are well characterized, whereas no information is available for the corresponding factors in archaeal and eukaryotic cells. Here we describe a protein found among the ribosome-associated proteins which dimerizes the 30S ri ...
DOI:
10.1016/j.bbrc.2022.01.043

16. DNA–Protein Cross-Linking Sequencing for Genome-Wide Mapping of Thymidine Glycol

Author:
Feng Tang; Jun Yuan; Bi-Feng Yuan; Yinsheng Wang
Source:
Journal of the American Chemical Society 2022 v.144 no.1 pp. 454-462
ISSN:
1520-5126
Subject:
polyacrylamide gel electrophoresis,   etc   ; DNA; DNA glycosylases; crosslinking; deamination; fractionation; genome; genomics; heterochromatin; humans; oxidation; thymidine;     Show all 12 Subjects
Abstract:
... Thymidine glycol (Tg) is the most prevalent form of oxidatively induced pyrimidine lesions in DNA. Tg can arise from direct oxidation of thymidine in DNA. In addition, 5-methyl-2′-deoxycytidine (5-mdC) can be oxidized to 5-mdC glycol, and its subsequent deamination also yields Tg. However, Tg's distribution in the human genome remains unknown. Here, we presented a DNA–protein cross-linking sequenc ...
DOI:
10.1021/jacs.1c10490

17. Determination of three sites involved in the divergence of L-aspartate-α-decarboxylase self-cleavage in bacteria

Author:
Man Zhao; Meinan Wang; Li Peng; Wei Liu; Xiangting Song; Zhiqiang Liu; Yuguo Zheng
Source:
Enzyme and microbial technology 2022 v.158 pp. 110048
ISSN:
0141-0229
Subject:
polyacrylamide gel electrophoresis,   etc   ; aspartic acid; catalytic activity; decarboxylation; enzymes; evolution; genes; site-directed mutagenesis; technology;     Show all 9 Subjects
Abstract:
... L-aspartate-α-decarboxylase (PanD) is an essential enzyme catalysing the decarboxylation of L-aspartate to β-alanine in organisms. To perform the catalytic functions, PanD pro-proteins need to be self-cleaved to form two subunits: active α-subunit and β-subunit. However, the processes of self-cleavage have diverged in different organisms for unknown reasons. To reveal the possible divergence mecha ...
DOI:
10.1016/j.enzmictec.2022.110048

18. Digestive propensity of Aflatoxin M1 (4‐Hydroxyaflatoxin B1), an indication from in vitro digestion model system

Author:
Lal Krishan Kumar; Ankita Kapri; Rajeev Chandel; Varun Kumar; Suryakant Verma; Gowdar Veerappa Vedamurthy; Dheer Singh; Suneel Kumar Onteru
Source:
Journal of food processing and preservation 2022 v.46 no.5 pp. e16577
ISSN:
0145-8892
Subject:
polyacrylamide gel electrophoresis,   etc   ; aflatoxin M1; carboxylic ester hydrolases; chemical pollutants; milk; milk quality; pancreatic juice;     Show all 7 Subjects
Abstract:
... Milk quality and safety maintenance is an important issue, as many reports showed several biological and chemical contaminants in the milk. Among all contaminants, AFM1 is detected frequently in milk samples. Therefore, our main aim was to understand the digestive fate of AFM1 in an in vitro digestion (IVD) model system by using AFM1 spiked milk samples (2 μg/L). The IVD of the milk was confirmed ...
DOI:
10.1111/jfpp.16577

19. A simple screening strategy for complex RNA-DNA hybrid nanoshapes

Author:
Shi Chen; Alba Monferrer; Thomas Hermann
Source:
Methods 2022 v.197 pp. 106-111
ISSN:
1046-2023
Subject:
gel electrophoresis,   etc   ; DNA; RNA;     Show all 3 Subjects
Abstract:
... The design of hybrid nucleic acid nanomaterials capitalizes on the partitioning of architectural and functional roles between structurally diverse RNA modules and chemically robust DNA components. Selecting optimal combinations of RNA and DNA building blocks is the key to preparing stable polygonal RNA-DNA hybrid nanoshapes. Here, we outline a simple screening strategy by gel electrophoresis under ...
DOI:
10.1016/j.ymeth.2021.02.015

20. A strain of Saccharomyces cerevisiae with high production of butanol

Author:
Yunxia Gong; Naranzul Tumurbaatar; Junying Zhou; Xiaobao Qi
Source:
European food research & technology 2022 v.248 no.1 pp. 207-217
ISSN:
1438-2377
Subject:
pulsed-field gel electrophoresis,   etc   ; Saccharomyces cerevisiae; butanol; dough; fermentation; flour; food research; glucose; maltose; pH; sucrose;     Show all 11 Subjects
Abstract:
... The paper was aimed to isolate a strain of Saccharomyces cerevisiae with higher alcohol, butanol. 32 strains of S. cerevisiae were isolated and identified from the dough starters from different regions. First, sterile flour solution (SFS) was developed as a substrate, and the HS-SPME-GC/MS technique was used to detect the volatile compounds, especially butanol. Then, gas production rate and acid-p ...
DOI:
10.1007/s00217-021-03874-z