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gelelectrophoresis, etc ; Escherichia coli; reporter genes; Show all 3 Subjects
Abstract:
... Amino acid transport systems perform important physiological functions; their role should certainly be considered in microbial production of amino acids. Typically, in the context of metabolic engineering, efforts are focused on the search for and application of specific amino acid efflux pumps. However, in addition, importers can also be used to improve the industrial process as a whole. In this ...
polyacrylamide gelelectrophoresis, etc ; allergens; autoclaving; epitopes; heat; peptides; proteomics; soybeans; Show all 8 Subjects
Abstract:
... The study investigated the changes in IgE binding capacity, protein profiles and peptide compositions after soybeans were boiled and autoclaved. The results of ELISA showed that the IgE binding capacity of soybean was reduced by 69.3% and 88.9% after boiling and autoclaving, respectively. Above 43 and 10 kDa proteins disappeared in boiled and autoclaved soybeans from SDS-PAGE, respectively. A Venn ...
polyacrylamide gelelectrophoresis, etc ; color; diamine oxidase; hydrogen peroxide; peroxidase; stabilizers; Show all 6 Subjects
Abstract:
... The existing zymography method for the detection of diamine oxidase (DAO) activity has been improved by a new staining procedure with the aim to ameliorate its sensitivity. Both procedures used SDS-PAGE gels containing uniformly distributed entrapped peroxidase (that wouldn't migrate during electrophoresis). The new approach with 3,5-dichloro-2-hydroxybenzenesulfonate (DCHBS) as peroxidase substra ...
polyacrylamide gelelectrophoresis, etc ; astringency; bioadhesives; emulsions; fluorescence; hydrophobicity; mucins; mucoadhesion; saliva; tongue; Show all 10 Subjects
Abstract:
... The interaction of emulsions with the tongue is key to the sensory appeal of food and can potentially be exploited for oral/buccal pharmaceutical delivery. Whilst there is good understanding of the different mucoadhesive forces governing emulsion interaction with the tongue, their relative importance is not well understood. In addition, the physical location of emulsions within the saliva papillae ...
polyacrylamide gelelectrophoresis, etc ; antioxidant activity; ficain; hydrolysis; liquid chromatography; peptides; solubility; whey; Show all 8 Subjects
Abstract:
... Enzymatic whey proteins concentrates (WPC) hydrolysis under optimal conditions of ficin crude extract (FCE) in two ratios E/S (0.5 and 1%) was studied, assessing the impact of hydrolysis on WPC by determination degree of hydrolysis (DH), Fast proteins liquid chromatography (FPLC), Tricine SDS-PAGE, antioxidant activity at sampling time beside, and technological properties (solubility, emulsifying ...
polyacrylamide gelelectrophoresis; two-dimensional gelelectrophoresis, etc ; milk; sulfates; whey; Show all 5 Subjects
Abstract:
... Over the years, a number of methodological variations in polyacrylamide gel electrophoresis (PAGE) have been attempted for the separation of milk proteins. Caseins are similar in size and differ very little in net charge from each other. Also, in heated milk, κ-casein not only complexes with whey proteins but also undergoes self-polymerisation. These cause problems in the resolution of milk protei ...
polyacrylamide gelelectrophoresis, etc ; aspergillopepsin; bentonite; disease prevention; enzymatic treatment; filterability; filtration; taste; wines; Show all 9 Subjects
Abstract:
... Wine haze often occurs after bottling due to insufficient protein stabilization and is often referred as a quality defect by customers. To prevent wine haze, bentonite is commonly used to remove certain protein fractions. Proteases may represent an alternative to bentonite, especially Aspergillopepsin. The aim of this work was to find out if there are advantages for winemaker by using an acid prot ...
polyacrylamide gelelectrophoresis, etc ; adhesion; gastrointestinal system; lithium chloride; lysozyme; phenol; protective effect; viability; Show all 8 Subjects
Abstract:
... The present study investigated whether the surface layer (S‐layer), which is known to have a varying effect from strain to strain on aggregation, adhesion ability, also has an effect on the resistance of bacteria to digestive enzymes, phenol, lysozymes. The effect of S‐layers on the resistance against various enzymes, aggregation and adhesion abilities, and strain specificity were determined of ei ...
gelelectrophoresis, etc ; Chlamydomonas reinhardtii; DNA; circular dichroism spectroscopy; fluorescence; nuclear magnetic resonance spectroscopy; telomeres; Show all 7 Subjects
Abstract:
... Competition among DNA non-canonical structures has been widely studied in repetitive DNA sequences. The factors affecting DNA structural polymorphism have always been an important area of research. The Chlamydomonas reinhardtii telomere (TTTTAGGG)ₙ is found to be an exception to the general idea of forming a folded G-quadruplex by a few repeats in any telomeric sequence. Herein, using gel electrop ...
polyacrylamide gelelectrophoresis, etc ; active ingredients; byproducts; encapsulation; fermentation; hydrophobicity; kafirins; multispectral imagery; prolamins; solubility; Show all 10 Subjects
Abstract:
... Prolamins have attracted increasing attention in recent years because of their potential application in active ingredient encapsulation and food packaging. As a byproduct of the Baijiu brewing industry with huge production (40 million tons every year), Jiuzao is a great source of prolamin because the fermentation process consumes mainly starch instead of protein. However, information on the extrac ...
gelelectrophoresis, etc ; analytical chemistry; antibodies; chromatography; complement; immunoassays; ionic strength; mass spectrometry; troponin I; Show all 9 Subjects
Abstract:
... Upconverting nanoparticles are attractive reporters for immunoassays, because their high specific activity and lack of autofluorescence background enable their detection at extremely low concentrations. However, the sensitivity achieved with heterogeneous sandwich immunoassays using nanoparticle reporters is generally limited by the nonspecific binding of nanoparticle antibody conjugates to solid ...
polyacrylamide gelelectrophoresis, etc ; alcohols; biorefining; choline; choline chloride; food chemistry; oat protein; oats; pH; Show all 9 Subjects
Abstract:
... The aim of this study is to investigate the effect of dihydric alcohol chain length (1,2-ethanediol, 1,3-propanediol, and 1,4-butanediol) on the structure of deep eutectic solvents (DESs) and the properties of the extracted oat proteins. Herein, five anhydrous and nine hydrated DESs were successfully prepared by mixing choline chloride, dihydric alcohol, and/or water in a heating method. The struc ...
gelelectrophoresis, etc ; DNA; Dependoparvovirus; capillary zone electrophoresis; quality control; transgenes; ultracentrifugation; viral genome; viruses; Show all 9 Subjects
Abstract:
... With recent FDA approval of two recombinant adeno‐associated virus (rAAV)‐based gene therapies, these vectors have proven that they are suitable to address monogenic diseases. However, rAAVs are relatively new modalities, and their production and therapy costs significantly exceed those of conventional biologics. Thus, significant efforts are made to improve the processes, methods, and techniques ...
polyacrylamide gelelectrophoresis, etc ; Trimeresurus; antivenoms; coagulation; community health; cross reaction; hemolysis; phospholipases; venoms; India; Show all 10 Subjects
Abstract:
... Green pit vipers, a name that can refer to several unrelated species, comprise a large group of venomous snakes found across the humid areas of tropical and sub-tropical Asia, and are responsible for most of the bite cases across this region. In India, green pit vipers belonging to several genera are prevalent in the northern and north-eastern hilly region, unrelated to species present in the peni ...
gelelectrophoresis, etc ; Pyrococcus furiosus; dimerization; liquid chromatography; protein synthesis; proteomics; research; ribosomes; tandem mass spectrometry; Show all 9 Subjects
Abstract:
... Ribosome dimerization is one of the bacterial events that suppresses protein synthesis in the stationary phase. Protein factors responsible for ribosome dimerization in bacteria are well characterized, whereas no information is available for the corresponding factors in archaeal and eukaryotic cells. Here we describe a protein found among the ribosome-associated proteins which dimerizes the 30S ri ...
polyacrylamide gelelectrophoresis, etc ; DNA; DNA glycosylases; crosslinking; deamination; fractionation; genome; genomics; heterochromatin; humans; oxidation; thymidine; Show all 12 Subjects
Abstract:
... Thymidine glycol (Tg) is the most prevalent form of oxidatively induced pyrimidine lesions in DNA. Tg can arise from direct oxidation of thymidine in DNA. In addition, 5-methyl-2′-deoxycytidine (5-mdC) can be oxidized to 5-mdC glycol, and its subsequent deamination also yields Tg. However, Tg's distribution in the human genome remains unknown. Here, we presented a DNA–protein cross-linking sequenc ...
polyacrylamide gelelectrophoresis, etc ; aspartic acid; catalytic activity; decarboxylation; enzymes; evolution; genes; site-directed mutagenesis; technology; Show all 9 Subjects
Abstract:
... L-aspartate-α-decarboxylase (PanD) is an essential enzyme catalysing the decarboxylation of L-aspartate to β-alanine in organisms. To perform the catalytic functions, PanD pro-proteins need to be self-cleaved to form two subunits: active α-subunit and β-subunit. However, the processes of self-cleavage have diverged in different organisms for unknown reasons. To reveal the possible divergence mecha ...
polyacrylamide gelelectrophoresis, etc ; aflatoxin M1; carboxylic ester hydrolases; chemical pollutants; milk; milk quality; pancreatic juice; Show all 7 Subjects
Abstract:
... Milk quality and safety maintenance is an important issue, as many reports showed several biological and chemical contaminants in the milk. Among all contaminants, AFM1 is detected frequently in milk samples. Therefore, our main aim was to understand the digestive fate of AFM1 in an in vitro digestion (IVD) model system by using AFM1 spiked milk samples (2 μg/L). The IVD of the milk was confirmed ...
... The design of hybrid nucleic acid nanomaterials capitalizes on the partitioning of architectural and functional roles between structurally diverse RNA modules and chemically robust DNA components. Selecting optimal combinations of RNA and DNA building blocks is the key to preparing stable polygonal RNA-DNA hybrid nanoshapes. Here, we outline a simple screening strategy by gel electrophoresis under ...
pulsed-field gelelectrophoresis, etc ; Saccharomyces cerevisiae; butanol; dough; fermentation; flour; food research; glucose; maltose; pH; sucrose; Show all 11 Subjects
Abstract:
... The paper was aimed to isolate a strain of Saccharomyces cerevisiae with higher alcohol, butanol. 32 strains of S. cerevisiae were isolated and identified from the dough starters from different regions. First, sterile flour solution (SFS) was developed as a substrate, and the HS-SPME-GC/MS technique was used to detect the volatile compounds, especially butanol. Then, gas production rate and acid-p ...