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1. The status of the terminal regions of α-synuclein in different forms of aggregates during fibrillization

Author:
Marvian, Amir Tayaranian; Aliakbari, Farhang; Mohammad-Beigi, Hossein; Ahmadi, Zeinab Alsadat; Mehrpooyan, Sina; Lermyte, Frederik; Nasouti, Mahour; Collingwood, Joanna F.; Otzen, Daniel E.; Morshedi, Dina
Source:
International journal of biological macromolecules 2020 v.155 pp. 543-550
ISSN:
0141-8130
Subject:
polyacrylamide gel electrophoresis,   etc   ; Parkinson disease; amyloid; fluorescence; models; neurons;     Show all 6 Subjects
Abstract:
... The α-synuclein (αSN) amyloid fibrillization process is known to be a crucial phenomenon associated with neuronal loss in various neurodegenerative diseases, most famously Parkinson's disease. The process involves different aggregated species and ultimately leads to formation of β-sheet rich fibrillar structures. Despite the essential role of αSN aggregation in the pathoetiology of various neurolo ...
DOI:
10.1016/j.ijbiomac.2020.03.238

2. Differential vs. comparative gel electrophoresis: New technology drives standardisation and quantification in protein two-dimensional gel electrophoresis

Author:
König, Simone
Source:
Trends in analytical chemistry 2020 v.122 pp. 115731
ISSN:
0165-9936
Subject:
two-dimensional gel electrophoresis,   etc   ; databases; fluorescence; gels; protein synthesis;     Show all 5 Subjects
Abstract:
... Two-dimensional gel electrophoresis (2DE) is a proven high-resolution separation technology in the protein laboratory. Nevertheless, ever since its invention the technique has had the drawback of low reproducibility. For protein expression analysis based on replicates, differential gel electrophoresis (DIGE) has, therefore, been invented. This technique is however not applicable for the comparison ...
DOI:
10.1016/j.trac.2019.115731

3. Differences in corona formation of catalase immobilised on gold and silver nanoparticles

Author:
Ranoszek-Soliwoda, Katarzyna; Czechowska, Ewa; Tomaszewska, Emilia; Pudlarz, Agnieszka; Szemraj, Janusz; Celichowski, Grzegorz; Grobelny, Jaroslaw
Source:
Colloids and surfaces 2020 v.600 pp. 125003
ISSN:
0927-7757
Subject:
polyacrylamide gel electrophoresis,   etc   ; adsorption; catalase; chemical composition; gold; models; moieties; nanoparticles; nanosilver; prediction; protein corona; silver;     Show all 12 Subjects
Abstract:
... The adsorption process of proteins on nanoparticles (NPs) is spontaneous and depends on many factors, including the NP composition, size, shape, dispersity, and surface chemistry, as well the protein type, its chemical structure, affinity to NP surface, size, shape, availability of functional groups, etc. As a result, prediction of the adsorption process and the surface coverage of NPs with protei ...
DOI:
10.1016/j.colsurfa.2020.125003

4. Peroxidase zymograms obtained by agarose native gel electrophoresis have unmet resolution and completeness

Author:
Oloketuyi, Sandra; Annovi, Giulia; de Marco, Ario
Source:
International journal of biological macromolecules 2020 v.156 pp. 869-873
ISSN:
0141-8130
Subject:
gel electrophoresis,   etc   ; acrylamides; agarose; biomarkers; gels; horseradish; peroxidase; protein isoforms; staining;     Show all 9 Subjects
Abstract:
... A recently published method to separate protein isoforms by means of a flat-bed agarose native gel was adapted for identifying simultaneously both acid and basic isoforms of plant peroxidases. These were evidenced by in situ activity staining using alternative substrates for which the isoforms showed specific preference. Such approach allowed the detection of a significantly higher number of horse ...
DOI:
10.1016/j.ijbiomac.2020.04.058

5. Development of an S-layer gene-based PCR-DGGE assay for monitoring dominant Lactobacillus helveticus strains in natural whey starters of Grana Padano cheese

Author:
Miragoli, Francesco; Patrone, Vania; Romaniello, Francesco; Rebecchi, Annalisa; Callegari, Maria Luisa
Source:
Food microbiology 2020 v.89 pp. 103457
ISSN:
0740-0020
Subject:
denaturing gradient gel electrophoresis,   etc   ; Grana cheese; Lactobacillus helveticus; cheesemaking; genes; loci; monitoring; starter cultures; whey;     Show all 9 Subjects
Abstract:
... Monitoring L. helveticus strain dynamics in natural whey starters is of great interest at the industrial level due to the key role that this bacterial population plays in Grana Padano cheese production. In this study, we aimed to develop a PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) assay based on the slpH locus, in parallel with performing culture-dependent analysis of whey samples usi ...
DOI:
10.1016/j.fm.2020.103457

6. Plasmids and genes contributing to high-level quinolone resistance in Escherichia coli

Author:
Vinué, Laura; Sater, Mohamad R.A.; Herriott, Ian C.; Huntley, Miriam H.; Wang, Minggui; Jacoby, George A.; Hooper, David C.
Source:
International journal of antimicrobial agents 2020 v.56 no.1 pp. 105987
ISSN:
0924-8579
Subject:
pulsed-field gel electrophoresis,   etc   ; Escherichia coli; ciprofloxacin; genes; nanopores; plasmids; transposons;     Show all 7 Subjects
Abstract:
... Introduction: The importance of plasmid-mediated quinolone resistance (PMQR) in Enterobacterales and its high incidence has been emphasised many times. However, a clinical strain carrying more than two PMQR genes is rare. This study sequenced plasmid transconjugants from a donor strain carrying four different PMQR genes to establish their genetic locations.Methods: An Escherichia coli clinical str ...
DOI:
10.1016/j.ijantimicag.2020.105987

7. Collagen fibrils of sea cucumber (Apostichopus japonicus) are heterotypic

Author:
Tian, Mo; Xue, Changhu; Chang, Yaoguang; Shen, Jingjing; Zhang, Yuying; Li, Zhaojie; Wang, Yanchao
Source:
Food chemistry 2020 v.316 pp. 126272
ISSN:
0308-8146
Subject:
polyacrylamide gel electrophoresis,   etc   ; Apostichopus japonicus; bioinformatics; collagen; food quality; integument; proteomics;     Show all 7 Subjects
Abstract:
... Sea cucumbers attracted increasing interest due to its nutritional functions. Collagen is the most important structural biomacromolecule in sea cucumber body wall, and is highly related to the textual properties and food quality of sea cucumber. In this study, the type of constituent collagens of sea cucumber collagen fibrils was investigated, employing a commercially important species Apostichopu ...
DOI:
10.1016/j.foodchem.2020.126272

8. Agarose native gel electrophoresis for characterization of antibodies

Author:
Li, Cynthia; Akuta, Teruo; Nakagawa, Masataka; Sato, Tomomi; Shibata, Takashi; Maruyama, Toshiaki; Okumura, C.J.; Kurosawa, Yasunori; Arakawa, Tsutomu
Source:
International journal of biological macromolecules 2020 v.151 pp. 885-890
ISSN:
0141-8130
Subject:
agar gel electrophoresis,   etc   ; agarose; blood serum; cell culture; electrodes; monoclonal antibodies; polyclonal antibodies;     Show all 7 Subjects
Abstract:
... This study was conducted to evaluate applicability of the previously reported native agarose gel electrophoresis to the analysis of various monoclonal and polyclonal antibodies. Experiments were carried to test the electrophoresis system for characterization of different monoclonal antibodies and animal serum, analysis of expressed antibodies in cell culture and evaluation of antibody stability. A ...
DOI:
10.1016/j.ijbiomac.2020.02.185

9. Thermal inactivation of jack bean urease

Author:
Illeová, Viera; Šefčík, Ján; Polakovič, Milan
Source:
International journal of biological macromolecules 2020 v.151 pp. 1084-1090
ISSN:
0141-8130
Subject:
gel electrophoresis,   etc   ; Canavalia ensiformis; enzyme activity; heat inactivation; hydrodynamics; indigenous species; light scattering; models; temperature; urease;     Show all 10 Subjects
Abstract:
... Thermal inactivation of oligomeric enzymes results in complex structural changes. This work deals with thermal inactivation of a native hexamer, jack bean urease. In order to find the mechanism and kinetics of thermal inactivation corresponding well with the modification of tertiary and quaternary structure of this enzyme, several types of experiments were carried out in the temperature range of 6 ...
DOI:
10.1016/j.ijbiomac.2019.10.150

10. Proteomic analysis of the phytogenic fungus Sclerotinia sclerotiorum

Author:
Otun, Sarah; Ntushelo, Khayalethu
Source:
Journal of chromatography 2020 v.1144 pp. 122053
ISSN:
1570-0232
Subject:
polyacrylamide gel electrophoresis,   etc   ; Sclerotinia sclerotiorum; chromatography; fungi; mycelium; plant pathogens; proteins; proteome; proteomics; sodium; spectrometers;     Show all 11 Subjects
Abstract:
... Sclerotinia sclerotiorum is a necrotrophic phytopathogen that has been the subject of several scientific research efforts. Despite the numerous research efforts its proteome remains understudied. This study aimed to identify proteins produced by S. sclerotiorum, thereby increasing the current proteomic knowledge base. Total proteins were extracted from mycelia scraped from five-day old cultures of ...
DOI:
10.1016/j.jchromb.2020.122053

11. Effect of the frying process on the properties of gluten protein of you-tiao

Author:
Zhou, Ruoxin; Sun, Juan; Qian, Haifeng; Li, Yan; Zhang, Hui; Qi, Xiguang; Wang, Li
Source:
Food chemistry 2020 v.310 pp. 125973
ISSN:
0308-8146
Subject:
polyacrylamide gel electrophoresis,   etc   ; disulfide bonds; frying; gluten; human nutrition; hydrophobic bonding; hydrophobicity; processing technology; wheat;     Show all 9 Subjects
Abstract:
... Wheat is one of the most important grains in cereal products. Gluten protein is an important component of wheat and plays an important role in the human diet. The variations of gluten protein in you-tiao were investigated in this study during frying, with a view toward a theoretical basis for the improvement of processing methods and quality in you-tiao. During the processing of you-tiao, gluten p ...
DOI:
10.1016/j.foodchem.2019.125973

12. Changes in strand 6B and helix B during neuroserpin inhibition: Implication in severity of clinical phenotype

Author:
Ali, Mohammad Farhan; Kaushik, Abhinav; Gupta, Dinesh; Ansari, Shoyab; Jairajpuri, Mohamad Aman
Source:
Biochimica et biophysica acta 2020 v.1868 no.4 pp. 140363
ISSN:
1570-9639
Subject:
polyacrylamide gel electrophoresis,   etc   ; brain; dementia; emissions factor; epilepsy; fluorescence; hydrophobicity; memory; phenotype; plasminogen activator; polymerization; serpins;     Show all 12 Subjects
Abstract:
... Neuroserpin (NS) is predominantly expressed in brain and inhibits tissue-type plasminogen activator (tPA) with implications in brain development and memory. Nature of conformational change in pathological variants in strand 6B and helix B of NS that cause a relatively mild to severe epilepsy (and/or dementia) remains largely elusive. MD simulation with wild type (WT) NS, strand 6B and helix B vari ...
DOI:
10.1016/j.bbapap.2020.140363

13. Facile Identification and Isolation of Protease Using SDS-PAGE and Zymography

Author:
Cho, Yun Ji; Park, Jong Ho; Chung, Goo Yong; Shin, Hwa Sung
Source:
Biotechnology and bioprocess engineering 2020 v.25 no.2 pp. 164-169
ISSN:
1226-8372
Subject:
polyacrylamide gel electrophoresis,   etc   ; drugs; gels; molecular weight; proteinases; proteolysis; staining; stroke; therapeutics;     Show all 9 Subjects
Abstract:
... Purification and identification of novel proteases is critical in the development of new therapeutic drugs, such as those for ischemic stroke. However, it's a time-consuming and expensive job to purify enzymes from natural sources. Furthermore, even if the process is completed, the enzyme could be a known protease. Zymography is a protease-confirming assay, but its molecular weight is hard to be a ...
DOI:
10.1007/s12257-019-0396-8

14. Development of a bacterial DNA extraction modular chip using a magnetic particle and portable vibration motor

Author:
Lim, Sun Young; Lee, Tae Jae; Shin, Seol Yi; Bae, Nam Ho; Lee, Seok Jae; Park, Yoo Min
Source:
Analytical methods 2020 v.12 no.9 pp. 1197-1202
ISSN:
1759-9679
Subject:
gel electrophoresis,   etc   ; DNA; Escherichia coli O157; magnetism; mixing; pathogens; quantitative polymerase chain reaction; vibration;     Show all 8 Subjects
Abstract:
... A novel modular chip-based bacterial DNA extraction device was developed to overcome limitations associated with conventional DNA extraction processes that involve lab-scale instrument systems. The DNA extraction modular chip consists of a core chamber, a magnetic jig, and a vibration jig. The vibration jig allows the precise blending of solutions such as a lysis buffer and target bacterial sample ...
DOI:
10.1039/c9ay02690h

15. Detection of bacteria in bovine ovarian follicular fluid

Author:
Salary, A.; Kafi, M.; Derakhshandeh, A.; Moezzi, M.S.
Source:
Letters in applied microbiology 2020 v.70 no.3 pp. 137-142
ISSN:
0266-8254
Subject:
pulsed-field gel electrophoresis,   etc   ; Escherichia coli; Staphylococcus aureus; anaerobes; bacteria; dairy cows; follicular fluid; lipopolysaccharides; uterus;     Show all 9 Subjects
Abstract:
... The aim of this study was to examine the sterility of follicular fluid in large ovarian follicles in dairy cows. In all, 17 samples of paired follicular fluids and uterine contents collected from post‐slaughtered dairy cows were cultured to detect aerobic and facultative anaerobic bacteria. Furthermore, the origin of the bacterial isolates from samples of follicular fluid and the uteri was also in ...
DOI:
10.1111/lam.13254

16. Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering

Author:
Arana-Peña, Sara; Rios, Nathalia S.; Mendez-Sanchez, Carmen; Lokha, Yuliya; Carballares, Diego; Gonçalves, Luciana R.B.; Fernandez-Lafuente, Roberto
Source:
International journal of biological macromolecules 2020 v.145 pp. 856-864
ISSN:
0141-8130
Subject:
polyacrylamide gel electrophoresis,   etc   ; Pseudozyma antarctica; biocatalysts; coatings; crosslinking; glutaraldehyde; immobilized enzymes; phospholipases; polyethyleneimine; triacylglycerol lipase;     Show all 10 Subjects
Abstract:
... This paper shows the step by step coimmobilization of up to five different enzymes following two different orders in the coimmobilization to alter the effect of substrate diffusion limitations. The enzymes were the lipases A and B from Candida antarctica, the lipases from Rhizomocur miehei and, Themomyces lanuginosus and the phospholipase Lecitase Ultra. The utilized strategy was a layer by layer ...
DOI:
10.1016/j.ijbiomac.2019.10.087

17. Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples

Author:
Anton, Milan; Moranova, Ludmila; Hrstka, Roman; Bartosik, Martin
Source:
Analytical methods 2020 v.12 no.6 pp. 822-829
ISSN:
1759-9679
Subject:
gel electrophoresis,   etc   ; Papillomaviridae; chronic diseases; electrochemistry; genotype; polymerase chain reaction; rapid methods; screening; uterine cervical neoplasms;     Show all 9 Subjects
Abstract:
... Cervical cancer is caused by a persistent infection with high-risk types of human papillomaviruses (HPVs), especially HPV16 and HPV18. HPV testing therefore represents an important alternative to standard cytological screening. However, existing HPV tests are often laborious, expensive or time-consuming, and thus novel techniques are developed that would be inexpensive, rapid, easy to perform and ...
DOI:
10.1039/c9ay02383f

18. Mass spectrometric approach for the analysis of the hard protein corona of nanoparticles in living cells

Author:
Szekeres, Gergo Peter; Fernández-Iglesias, Nerea; Kneipp, Janina; Montes-Bayón, Maria; Bettmer, Jörg
Source:
Journal of proteomics 2020 v.212 pp. 103582
ISSN:
1874-3919
Subject:
polyacrylamide gel electrophoresis,   etc   ; cell culture; human cell lines; macrophages; mass spectrometry; nanogold; nanoparticles; protein corona; therapeutics;     Show all 9 Subjects
Abstract:
... The diagnostic and therapeutic application of nanoparticles requires comprehensive knowledge of their interaction with the biomolecular surroundings. The formation of the protein corona on nanoparticles that were internalized by living cells is yet to be understood. In this study, we present a robust approach for the electrophoretic and mass spectrometric analysis of the hard protein corona compos ...
DOI:
10.1016/j.jprot.2019.103582

19. Genetic Relatedness Among Streptococcus agalactiae Isolated from Cattle, Fish, and Humans

Author:
Boonyayatra, Sukolrat; Wongsathein, Dilok; Tharavichitkul, Prasit
Source:
Foodborne pathogens & disease 2020 v.17 no.2 pp. 137-143
ISSN:
1556-7125
Subject:
pulsed-field gel electrophoresis,   etc   ; Streptococcus agalactiae; cattle; fish; genes; genetic relationships; multilocus sequence typing;     Show all 7 Subjects
Abstract:
... Streptococcus agalactiae is well recognized to cause a variety of infections in many animal species and humans. We aimed to investigate genetic relatedness of S. agalactiae strains isolated from humans and animal origins, including cattle and fish, using capsular gene typing, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing techniques. Our results revealed that S. agalactiae ...
DOI:
10.1089/fpd.2019.2687

20. Medium-throughput in vitro detection of DNA cleavage by CRISPR-Cas12a

Author:
Creutzburg, Sjoerd C.A.; Swartjes, Thomas; van der Oost, John
Source:
Methods 2020 v.172 pp. 27-31
ISSN:
1046-2023
Subject:
agar gel electrophoresis,   etc   ; CRISPR-Cas systems; DNA damage; agarose; biotin; fluorescence; fluorescent dyes; gels; nucleases; streptavidin;     Show all 10 Subjects
Abstract:
... Quantifying DNA cleavage by CRISPR-Cas nucleases is usually done by separating the cleaved products from the non-cleaved target by agarose gel electrophoresis. We devised a method that eliminates the quantification from band intensity on agarose gel, and uses a target with a fluorescent dye on the one end and a biotin on the other. Cleavage of the target will separate the dye from the biotin, and ...
DOI:
10.1016/j.ymeth.2019.11.005