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1. Differential vs. comparative gel electrophoresis: New technology drives standardisation and quantification in protein two-dimensional gel electrophoresis

Author:
König, Simone
Source:
Trends in analytical chemistry 2020 v.122 pp. 115731
ISSN:
0165-9936
Subject:
gels; two-dimensional gel electrophoresis,   etc   ; databases; fluorescence; protein synthesis;     Show all 5 Subjects
Abstract:
... Two-dimensional gel electrophoresis (2DE) is a proven high-resolution separation technology in the protein laboratory. Nevertheless, ever since its invention the technique has had the drawback of low reproducibility. For protein expression analysis based on replicates, differential gel electrophoresis (DIGE) has, therefore, been invented. This technique is however not applicable for the comparison ...
DOI:
10.1016/j.trac.2019.115731

2. Multiphase Viscoplastic Flows in a Nonuniform Hele-Shaw Cell: A Fluidic Device to Control Interfacial Patterns

Author:
Eslami, Ali; Basak, Raunak; Taghavi, Seyed Mohammad
Source:
Industrial & engineering chemistry process design and development 2020 v.59 no.9 pp. 4119-4133
ISSN:
1520-5045
Subject:
gels,   etc   ; air; interface phenomena; viscosity;     Show all 4 Subjects
Abstract:
... Inspired by the problem of the removal of in situ gel-like materials via a displacement flow process, we use a fluidic device known as Hele-Shaw cell to study the flow parameters related to the displacement flow efficiency. In a displacement flow process, when a less viscous fluid displaces a more viscous fluid, exotic interfacial patterns known as viscous fingering appear. Motivated by the recent ...
DOI:
10.1021/acs.iecr.9b06064

3. The effect of DNA recovery on the subsequent quality of latent fingermarks: A pseudo-operational trial

Author:
Fieldhouse, S.; Parsons, R.; Bleay, S.; Walton-Williams, L.
Source:
Forensic science international 2020 v.307 pp. 110076
ISSN:
0379-0738
Subject:
gels,   etc   ; DNA; application parameters; crime;     Show all 4 Subjects
Abstract:
... The recovery of fingermarks and DNA from the same location at a crime scene can be problematic because of contamination issues associated with powdering or laboratory-based visualisation processes and/or the perceived destructive impact of commonly employed ‘swabbing’ approaches to DNA recovery. Previous research in a controlled environment demonstrated that it was possible to recover DNA and late ...
DOI:
10.1016/j.forsciint.2019.110076

4. A dynamic self-regulation actuator combined double network gel with gradient structure driven by chemical oscillating reaction

Author:
Li, Jie; Li, Xiuchen; Zheng, Zhaohui; Ding, Xiaobin
Source:
RSC advances 2019 v.9 no.23 pp. 13168-13172
ISSN:
2046-2069
Subject:
gels
Abstract:
... Self-regulation of the dynamic actuation of a chemical oscillating reaction-based gel was realized by altering the network structure of the gradient double network gel. We demonstrated that the propagation mode of the chemical wave was influenced by the network structure, and consequently determined the dynamic feature of the gel actuator. ...
DOI:
10.1039/c9ra02340b

5. Structural evolution of binder gel in alkali-activated cements exposed to electrically accelerated leaching conditions

Author:
Gook Jang, Jeong; Park, Solmoi; Yoon, H.N.; Seo, Joonho; Lee, H.K.; Jang, Jeong Gook
Source:
Journal of hazardous materials 2020 v.387 pp. 121825
ISSN:
0304-3894
Subject:
gels,   etc   ; durability; fly ash; leaching; slags;     Show all 5 Subjects
Abstract:
... The structural evolution of a binder gel in alkali-activated cements exposed to accelerated leaching conditions is investigated for the first time. Samples incorporating fly ash and/or slag were synthesized and were exposed to electrically accelerated leaching by applying a current density of 5 A/m². The leaching behavior of the samples greatly depended on the binder gel formed in the samples. The ...
DOI:
10.1016/j.jhazmat.2019.121825

6. Unexpected microphase transitions in flow towards nematic order of cellulose nanocrystals

Author:
Kádár, Roland; Fazilati, Mina; Nypelö, Tiina
Source:
Cellulose 2020 v.27 no.4 pp. 2003-2014
ISSN:
0969-0239
Subject:
gels,   etc   ; cellulose; liquid crystals; nanocrystals; nanoparticles; rheology;     Show all 6 Subjects
Abstract:
... Organization of nanoparticles is essential in order to control their light-matter interactions. We present cellulose nanocrystal suspension organization in flow towards a unidirectional state. Visualization of evolving polarization patterns of the cellulose nanocrystal suspensions is combined with steady and oscillatory shear rheology. Elucidation of the chiral nematic mesophase in a continuous pr ...
DOI:
10.1007/s10570-019-02888-x

7. Cryo-XPS – A new technique for the quantitative analysis of the structure of electric double layer at colloidal particles?

Author:
Škvarla, Jiří; Kaňuchová, Mária; Shchukarev, Andrey; Girová, Anna; Brezáni, Ivan
Source:
Colloids and surfaces 2020 v.586 pp. 124234
ISSN:
0927-7757
Subject:
gels,   etc   ; colloids; prediction; quantitative analysis; silica; uncertainty;     Show all 6 Subjects
Abstract:
... There is a long-lasting uncertainty as to the structure of the metal oxide/water interface. Various structures have especially been invoked to rationalize the seemingly anomalous silica/water interface. But, none of them seems to be approved in a quantitative and systematic way. We show that the concentration ratio of monovalent counterions and coions around colloidal silica spheres, as detected b ...
DOI:
10.1016/j.colsurfa.2019.124234

8. Thermal gelling properties and structural properties of myofibrillar protein including thermo-reversible and thermo-irreversible curdlan gels

Author:
Jiang, Shuai; Zhao, Shenchi; Jia, Xiwen; Wang, Hui; Zhang, Huan; Liu, Qian; Kong, Baohua
Source:
Food chemistry 2020 v.311 pp. 126018
ISSN:
0308-8146
Subject:
gel strength; gels,   etc   ; curdlan; gelation; particle size; temperature; water holding capacity;     Show all 7 Subjects
Abstract:
... This study investigated the gelling and structural properties of composite gels prepared with myofibrillar protein (MP) and different concentrations of thermo-reversible curdlan gels (TRC) and thermo-irreversible curdlan gels (TIRC). The gel strength, water holding capacity, and whiteness of MP gels were significantly increased with increasing TRC and TIRC content (P < 0.05). Dynamic rheological t ...
DOI:
10.1016/j.foodchem.2019.126018

9. Molecular insights into the impacts of iron(III) ions on membrane fouling by alginate

Author:
Chen, Yifeng; Teng, Jiaheng; Liao, Bao-Qiang; Li, Renjie; Lin, Hongjun
Source:
Chemosphere 2020 v.242 pp. 125232
ISSN:
0045-6535
Subject:
gel chromatography; gels,   etc   ; crosslinking; density functional theory; filtration; fouling; ions; iron; microstructure; moieties; pH; sodium alginate;     Show all 12 Subjects
Abstract:
... Molecular mechanisms responsible for the filtration behaviors of sodium alginate (SA) in presence of different iron(III) ion concentration were explored in this study. It was found that specific filtration resistance (SFR) of alginate mixtures (1.0 gSA/L) firstly increased and then decreased to a trough with iron(III) concentration increase from 0 to 2.5 mM. Alginate mixture interacting with 0.1 m ...
DOI:
10.1016/j.chemosphere.2019.125232

10. Medium-throughput in vitro detection of DNA cleavage by CRISPR-Cas12a

Author:
Creutzburg, Sjoerd C.A.; Swartjes, Thomas; van der Oost, John
Source:
Methods 2020 v.172 pp. 27-31
ISSN:
1046-2023
Subject:
agar gel electrophoresis; gels,   etc   ; CRISPR-Cas systems; DNA damage; agarose; biotin; fluorescence; fluorescent dyes; nucleases; streptavidin;     Show all 10 Subjects
Abstract:
... Quantifying DNA cleavage by CRISPR-Cas nucleases is usually done by separating the cleaved products from the non-cleaved target by agarose gel electrophoresis. We devised a method that eliminates the quantification from band intensity on agarose gel, and uses a target with a fluorescent dye on the one end and a biotin on the other. Cleavage of the target will separate the dye from the biotin, and ...
DOI:
10.1016/j.ymeth.2019.11.005

11. Identification of sperm‐specific proteins associated with high fertility of Punjab Red and RIR x Local cross roosters with SDS‐PAGE and Immunoblotting

Author:
Mavi, Gurjot Kaur; Dubey, Prem P.; Cheema, Ranjna S.
Source:
Reproduction in domestic animals 2020 v.55 no.2 pp. 127-136
ISSN:
0936-6768
Subject:
gels; polyacrylamide gel electrophoresis,   etc   ; acrylamides; breeds; immunoblotting; immunoglobulin G; polyclonal antibodies; rabbits; roosters; spermatozoa;     Show all 10 Subjects
Abstract:
... The present study aimed at characterization of fertility‐associated proteins in PR and RIR x Local roosters and was conducted on two generations of birds. Roosters were divided into high‐ (>50%) and low‐fertility groups (<50%) based on sperm function tests and fertility rate in both the generations. Polyclonal antibodies were raised in rabbits against sperm proteins of first generation highly fert ...
DOI:
10.1111/rda.13593

12. Effect of microbial transglutaminase on the mechanical properties and microstructure of acid-induced gels and emulsion gels produced from thermal denatured egg white proteins

Author:
Alavi, Farhad; Emam-Djomeh, Zahra; Salami, Maryam; Mohammadian, Mehdi
Source:
International journal of biological macromolecules 2020 v.153 pp. 523-532
ISSN:
0141-8130
Subject:
gel strength; gels; polyacrylamide gel electrophoresis,   etc   ; crosslinking; egg albumen; emulsions; mechanical properties; mechanical stress; microstructure; modulus of elasticity; pH; protein-glutamine gamma-glutamyltransferase; solubility; tissue engineering;     Show all 14 Subjects
Abstract:
... In the current study, the impact of microbial transglutaminase (MTGase)-mediated crosslinking on the thermally denatured egg white protein (TD-EWP) made in alkaline pH in relation to their gelling properties of in gel and emulsion gel systems was investigated. Unlike native EWP that was not a good substrate for MTGase due to its compact structure, SDS-PAGE and gel solubility data showed the TD-EWP ...
DOI:
10.1016/j.ijbiomac.2020.03.008

13. A study of the physicochemical properties of rabbit glycated myofibrillary protein with high solubility in low ionic strength medium

Author:
Li, Shaobo; He, Zhifei; Li, Mingqi; Li, Ranran; Lu, Jingzhi; Li, Hongjun
Source:
International journal of biological macromolecules 2020 v.147 pp. 241-249
ISSN:
0141-8130
Subject:
gel strength; gels; polyacrylamide gel electrophoresis,   etc   ; denaturation; emulsifying; emulsifying properties; foaming; foaming properties; gelation; glycation; ionic strength; pH; rabbits; solubility; storage modulus; temperature; thermal stability;     Show all 17 Subjects
Abstract:
... To analyze the physicochemical properties of rabbit glycated myofibrillary protein (GMP) with different solubility ((15.10 ± 0.76)%, (35.03 ± 1.01)%, (55.06 ± 1.25)%, and (75.07 ± 1.86)%) in low ionic strength medium, the changes in SDS-PAGE, foaming properties, emulsifying properties, gelling properties, rheological properties, and thermal stability under different pH conditions were determined. ...
DOI:
10.1016/j.ijbiomac.2020.01.069

14. Hydrolysis by Indigenous Plasmin: Consequences for Enzymatic Cross-Linking and Acid-Induced Gel Formation of Non-Micellar Casein

Author:
Raak, Norbert; Brehm, Lena; Leidner, Rebecca; Henle, Thomas; Rohm, Harald; Jaros, Doris
Source:
Food biophysics 2020 v.15 no.1 pp. 32-41
ISSN:
1557-1858
Subject:
gel chromatography; gel electrophoresis; gels,   etc   ; buffers; casein; crosslinking; hydrolysis; lysine; nutritive value; pH; phosphates; plasmin; polypeptides; protein-glutamine gamma-glutamyltransferase; rheology; sodium caseinate;     Show all 16 Subjects
Abstract:
... Casein is a group of milk proteins with high nutritional value, and the exploitation of its techno-functional potentials has been investigated for decades. In this study, acid casein powder was dissolved in 0.1 mol/L phosphate buffers with different pH, resulting in casein solutions with pH 5.9, 6.6 and 7.3. During preparation and storage (40 °C) of the samples, casein hydrolysis was observed in s ...
DOI:
10.1007/s11483-019-09601-2

15. Impact of raw ham quality and tumbling time on the technological properties of polyphosphate-free cooked ham

Author:
Steen, Liselot; Neyrinck, Ellen; De Mey, Eveline; De Grande, Annatachja; Telleir, Danny; Raes, Katleen; Paelinck, Hubert; Fraeye, Ilse
Source:
Meat science 2020 v.164 pp. 108093
ISSN:
0309-1740
Subject:
gels,   etc   ; cooked foods; ham; hardness; pork quality; water holding capacity;     Show all 6 Subjects
Abstract:
... The effect of tumbling time (5 h30, 19 h and 26 h) and raw ham quality (superior, inferior or mixed quality) on the quality of polyphosphate-free cooked ham was investigated. The water holding capacity and total yield of the polyphosphate-free tumbled hams were dependent on both tumbling time and ham quality. Higher values of both parameters were obtained with an increase in tumbling time from 5 h ...
DOI:
10.1016/j.meatsci.2020.108093

16. Properties of lotus seed starch-glycerin monostearin V-complexes after long-term retrogradation

Author:
Zheng, Yixin; Wang, Bailong; Guo, Zebin; Zhang, Yi; Zheng, Baodong; Zeng, Shaoxiao; Zeng, Hongliang
Source:
Food chemistry 2020 v.311 pp. 125887
ISSN:
0308-8146
Subject:
gels,   etc   ; crystal structure; enthalpy; retrogradation; starch; thermosensitivity; viscoelasticity;     Show all 7 Subjects
Abstract:
... The properties of lotus seed starch-glycerin monostearin with V₆II and V₆I-complexes formed at 50 MPa and 100 MPa after long-term retrogradation (named as LS-GMS-50 and LS-GMS-100, respectively) were investigated. The results indicated LS-GMS-50 and LS-GMS-100 were conducive to the formation of crystallinity and an ordered structure of starch compared to lotus seed, lotus seed at 50 MPa and 100 MP ...
DOI:
10.1016/j.foodchem.2019.125887

17. Histidine Proton Shuttle-Initiated Switchable Inversion of Circularly Polarized Luminescence

Author:
Niu, Dian; Ji, Lukang; Ouyang, Guanghui; Liu, Minghua
Source:
ACS applied materials & interfaces 2020 v.12 no.15 pp. 18148-18156
ISSN:
1944-8252
Subject:
gels,   etc   ; deprotonation; enzymes; histidine; hydrogen bonding; luminescence; optical isomerism; protonation;     Show all 8 Subjects
Abstract:
... Switchable inversion of the sign of circularly polarized luminescence (CPL) in chiral supramolecular systems has gained remarkable interest because of its role in understanding the chirality-switching phenomena in biological systems and developing smart chiral luminescent materials. Herein, inspired by the histidine proton shuttle in natural enzymes, we synthesized a histidine π-gel (PyC₃H) and re ...
DOI:
10.1021/acsami.0c02080

18. Ba0.1Sr0.9Zr0.18Ti0.82O3 ceramics: dielectric properties and energy storage density under external electric field and temperature

Author:
Zhang, Tao; Aziguli, Haibibu; Wu, Yanhong; Yin, Jie; Yu, Ping
Source:
Journal of materials science 2020 v.55 no.7 pp. 2764-2771
ISSN:
0022-2461
Subject:
sol-gel processing,   etc   ; ceramics; dielectric properties; electric field; energy; temperature;     Show all 6 Subjects
Abstract:
... In order to develop the application of energy storage ceramics, the dielectric properties and energy storage density of Ba₀.₁Sr₀.₉Zr₀.₁₈Ti₀.₈₂O₃ ceramics (BSZT-118) prepared by the modified sol–gel process were researched under different temperatures under electric field. The results show that the dielectric constant of BSZT-118 is consistent with Johnson’s formula based on Devonshire phenomenolog ...
DOI:
10.1007/s10853-019-04248-3

19. B/N co-doped graphene oxide gel with extremely-high mobility and ION/IOFF for large-area field effect transistors

Author:
Novodchuk, I.; Kayaharman, M.; Ibrahim, K.; Al-Tuairqi, S.; Irannejad, M.; Abdel-Rahman, E.; Sanderson, J.; Bajcsy, M.; Yavuz, M.
Source:
Carbon 2020 v.158 pp. 624-630
ISSN:
0008-6223
Subject:
gels,   etc   ; chemical reactions; films (materials); graphene; graphene oxide; transistors;     Show all 6 Subjects
Abstract:
... Thin films of large-area graphene and graphene-based materials are highly desired for electrical applications. However, the current state-of-art synthesis methods produce large-area graphene films with multiple grain boundaries (GBs) that highly hinder their charge carrier mobilities and Iₒₙ/Iₒff ratios. Here, we demonstrate a femtosecond laser ablation process to produce B and N co-doped graphene ...
DOI:
10.1016/j.carbon.2019.11.034

20. Enhancement of ionic conductivity in Li0.5La0.5TiO3 with Ag nanoparticles

Author:
Ling, Ming’en; Jiang, Yue; Huang, Yi; Zhou, Yuxi; Zhu, Xiaohong
Source:
Journal of materials science 2020 v.55 no.9 pp. 3750-3759
ISSN:
0022-2461
Subject:
sol-gel processing,   etc   ; electrolytes; melting point; nanosilver; oxidation; silver;     Show all 6 Subjects
Abstract:
... Li₀.₅La₀.₅TiO₃ (LLTO)/nano-Ag composite electrolytes were synthesized by a sol–gel process. Through the addition of nano-Ag powder with a relatively low melting point, the lattice parameter, grain size and morphology of LLTO-based electrolytes were tailored. The results revealed that Ag was oxidized into Ag⁺ during high-temperature sintering in air, and partial Ag⁺ entered into LLTO lattice and im ...
DOI:
10.1007/s10853-019-04180-6