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1. Correction to: Gene overexpression screen for chromosome instability in yeast primarily identifies cell cycle progression genes

Author:
Tutaj, Hanna; Pogoda, Elzbieta; Tomala, Katarzyna; Korona, Ryszard
Source:
Current genetics 2019 v.65 no.2 pp. 493
ISSN:
0172-8083
Subject:
gene overexpression,   etc   ; cell cycle; chromosomes; genes; yeasts;     Show all 5 Subjects
Abstract:
... In the original publication, ‘Frumkin JP et al.’ reference was missed to include in the reference list. The complete reference should read as below. ...
DOI:
10.1007/s00294-018-0893-x

2. Tau Modulates VGluT1 Expression

Author:
Siano, Giacomo; Varisco, Martina; Caiazza, Maria Claudia; Quercioli, Valentina; Mainardi, Marco; Ippolito, Chiara; Cattaneo, Antonino; Di Primio, Cristina
Source:
Journal of molecular biology 2019 v.431 no.4 pp. 873-884
ISSN:
0022-2836
Subject:
gene overexpression,   etc   ; dementia; genes; microtubules; mutation; neurodegenerative diseases; synaptic transmission;     Show all 7 Subjects
Abstract:
... Tau displacement from microtubules is the first step in the onset of tauopathies and is followed by toxic protein aggregation. However, other non-canonical functions of Tau might have a role in these pathologies. Here, we demonstrate that a small amount of Tau localizes in the nuclear compartment and accumulates in both the soluble and chromatin-bound fractions. We show that favoring Tau nuclear t ...
DOI:
10.1016/j.jmb.2019.01.023

3. Corrections to: Enhanced lincomycin production by co‑overexpression of metK1 and metK2 in Streptomyces lincolnensis

Author:
Xu, Yurong; Tan, Guoqing; Ke, Meilan; Li, Jie; Tang, Yaqian; Meng, Sitong; Niu, Jingjing; Wang, Yansheng; Liu, Ruihua; Wu, Hang; Bai, Linquan; Zhang, Lixin; Zhang, Buchang
Source:
Journal of industrial microbiology & biotechnology 2018 v.45 no.6 pp. 447-448
ISSN:
1367-5435
Subject:
gene overexpression,   etc   ; Streptomyces lincolnensis; lincomycin;     Show all 3 Subjects
Abstract:
... In the online published article, row value “pIB139-metK1-metK2” in table 1 has been processed incorrectly. The correct table is given below ...
DOI:
10.1007/s10295-018-2036-2

4. An auto-inducible phosphate-controlled expression system of Bacillus licheniformis

Author:
Trung, Nguyen Thanh; Hung, Nguyen Minh; Thuan, Nguyen Huy; Canh, Nguyen Xuan; Schweder, Thomas; Jürgen, Britta
Source:
BMC biotechnology 2019 v.19 no.1 pp. 3
ISSN:
1472-6750
Subject:
gene overexpression,   etc   ; Bacillus licheniformis; batch fermentation; culture media; genes; models; phosphates; phytases; phytic acid; proteins;     Show all 10 Subjects
Abstract:
... BACKGROUND: A promoter that drives high-level, long-term expression of the target gene under substrate limited growth conditions in the absence of an artificial inducer would facilitate the efficient production of heterologous proteins at low cost. A novel phosphate-regulated expression system was constructed using the promoter of the phytase encoding gene phyL from Bacillus licheniformis for the ...
DOI:
10.1186/s12896-018-0490-6

5. Biosynthesis of nerol from glucose in the metabolic engineered Escherichia coli

Author:
Zong, Zhen; Hua, Qingsong; Tong, Xinyu; Li, Dongsheng; Wang, Chao; Guo, Daoyi; Liu, Zhijie
Source:
Bioresource technology 2019 v.287 pp. 121410
ISSN:
0960-8524
Subject:
gene overexpression,   etc   ; Escherichia coli; biosynthesis; catalytic activity; enzymes; genes; glucose; metabolic engineering; nerol;     Show all 9 Subjects
Abstract:
... In this study, nerol was biosynthesized in the metabolic engineered Escherichia coli from glucose for the first time. Firstly, the truncated neryl diphosphate synthase gene tNDPS1 was expressed that catalyzes isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) to form neryl diphosphate (NPP), and then the nerol synthase gene GmNES was co-expressed to synthesize the final product ne ...
DOI:
10.1016/j.biortech.2019.121410

6. Over-expression of Vitreoscilla hemoglobin (VHb) and flavohemoglobin (FHb) genes greatly enhances pullulan production

Author:
Xue, Si-Jia; Jiang, Hong; Chen, Lu; Ge, Na; Liu, Guang-Lei; Hu, Zhong; Chi, Zhen-Ming; Chi, Zhe
Source:
International journal of biological macromolecules 2019 v.132 pp. 701-709
ISSN:
0141-8130
Subject:
gene overexpression,   etc   ; Aureobasidium; Vitreoscilla; cell growth; fermentation; genes; hemoglobin; oxygen; pullulan; sucrose; transcription (genetics);     Show all 11 Subjects
Abstract:
... Overexpression of the optimized Vitreoscilla hemoglobin (VHb) gene and the native flavohemoglobin (FHb) gene in Aureobasidium melanogenum P16 rendered a V6 strain and a F44 strain to overproduce pullulan compared to that produced by their wild type strain P16. The capacity to bind CO and oxygen in the V6 strain and the F44 strain was also obviously enhanced. At the same time, the transcriptional l ...
DOI:
10.1016/j.ijbiomac.2019.04.007

7. Homologous overexpression of hydrogenase and glycerol dehydrogenase in Clostridium pasteurianum to enhance hydrogen production from crude glycerol

Author:
Sarma, Shyamali; Ortega, David; Minton, Nigel P.; Dubey, Vikash Kumar; Moholkar, Vijayanand S.
Source:
Bioresource technology 2019 v.284 pp. 168-177
ISSN:
0960-8524
Subject:
gene overexpression,   etc   ; Clostridium pasteurianum; biotransformation; genes; genetic engineering; glycerol; glycerol dehydrogenase; hydrogen; hydrogen production;     Show all 9 Subjects
Abstract:
... This study reports engineering of a hypertransformable variant of C. pasteurianum for bioconversion of glycerol into hydrogen (H2). A functional glycerol-triggered hydrogen pathway was engineered based on two approaches: (1) increasing product yield by overexpression of immediate enzyme catalyzing H2 production, (2) increasing substrate uptake by overexpression of enzymes involved in glycerol util ...
DOI:
10.1016/j.biortech.2019.03.074

8. Manipulation of polyhydroxyalkanoate granular sizes in Halomonas bluephagenesis

Author:
Shen, Rui; Ning, Zhi-Yu; Lan, Yu-Xuan; Chen, Jin-Chun; Chen, Guo-Qiang
Source:
Metabolic engineering 2019 v.54 pp. 117-126
ISSN:
1096-7176
Subject:
gene overexpression,   etc   ; Halomonas; bacteria; cell biology; culture media; engineering; genes; granules; molecular weight; mutants; polyhydroxyalkanoates; proteins;     Show all 12 Subjects
Abstract:
... Bacterial polyhydroxyalkanoates (PHA) are a family of intracellular polyester granules with sizes ranging from 100 to 500 nm. Due to their small sizes, it has been very difficult to separate the PHA granules from the bacterial broths. This study aims to engineer the PHA size control mechanism to obtain large PHA granular sizes beneficial for the separation. It has been reported that phasin (PhaP) ...
DOI:
10.1016/j.ymben.2019.03.011

9. Overexpression and repression of the tyrosinase gene in Lentinula edodes using the pChG vector

Author:
Sato, Toshitsugu; Takahashi, Machiko; Hasegawa, Junji; Watanabe, Hisayuki
Source:
Journal of bioscience and bioengineering 2019 v.128 no.1 pp. 1-7
ISSN:
1389-1723
Subject:
gene overexpression,   etc   ; Lentinula edodes; clones; enzyme activity; fruiting bodies; genes; glyceraldehyde-3-phosphate dehydrogenase; mycelium; protoplasts;     Show all 9 Subjects
Abstract:
... Tyrosinase is an industrially useful enzyme, however, it causes gill browning of Lentinula edodes fruiting bodies during preservation. In this study, we constructed two vectors, pChG-gTs and pChG-gTa, expressing sense and antisense tyrosinase gene of L. edodes, respectively, using promoters derived from the glyceraldehyde-3-phosphate dehydrogenase gene. The host strain SR-1 of L. edodes was select ...
DOI:
10.1016/j.jbiosc.2018.12.013

10. MicroRNA 4651 regulates nonsense-mediated mRNA decay by targeting SMG9 mRNA

Author:
Tan, Yanjie; Ma, Zhenfa; Jin, Yi; Zong, Ruojun; Wu, Jian; Ren, Zhuqing
Source:
Gene 2019 v.701 pp. 65-71
ISSN:
0378-1119
Subject:
gene overexpression,   etc   ; messenger RNA; microRNA; monitoring; protein synthesis; small interfering RNA; stop codon;     Show all 7 Subjects
Abstract:
... Nonsense-mediated mRNA decay (NMD) is originally identified as a conserved RNA surveillance mechanism that rapidly degrades aberrant mRNA containing premature termination codons (PTCs). However, the molecular regulation mechanisms by which microRNAs inhibit NMD has not been well understood. Here we identified that miR-4651 participated in the NMD pathway by downregulating expression levels of SMG9 ...
DOI:
10.1016/j.gene.2019.03.031

11. Overproduction of a β-fructofuranosidase1 with a high FOS synthesis activity for efficient biosynthesis of fructooligosaccharides

Author:
Aung, Thu; Jiang, Hong; Liu, Guang-Lei; Chi, Zhe; Hu, Zhong; Chi, Zhen-Ming
Source:
International journal of biological macromolecules 2019 v.130 pp. 988-996
ISSN:
0141-8130
Subject:
gene overexpression,   etc   ; Aureobasidium; beta-fructofuranosidase; biosynthesis; biotechnology; enzyme activity; fructooligosaccharides; genes; glycosylation; molecular weight; sucrose; yeasts;     Show all 12 Subjects
Abstract:
... Aureobasidium melanogenum 11–1 was found to be able to produce over 281.7 ± 7.1 U/mL of β-fructofuranosidase activity. The protein deduced from the cloned β-fructofuranosidase1 gene had the conserved motif A (IGDP), motif D (RDP) and motif E (ET) and 11 N-glycosylation sites, indicating it was a β-fructofuranosidase with the high-level fructooligosaccharide (FOS) biosynthesis. Overexpression of th ...
DOI:
10.1016/j.ijbiomac.2019.03.039

12. Bmp signaling in molar cusp formation

Author:
Meguro, Fumiya; Porntaveetus, Thantrira; Kawasaki, Maiko; Kawasaki, Katsushige; Yamada, Akane; Kakihara, Yoshito; Saeki, Makio; Tabeta, Koichi; Kessler, John A.; Maeda, Takeyasu; Ohazama, Atsushi
Source:
Gene expression patterns 2019 v.32 pp. 67-71
ISSN:
1567-133X
Subject:
gene overexpression,   etc   ; bone morphogenetic proteins; in situ hybridization; mice; teeth; transgenic animals;     Show all 6 Subjects
Abstract:
... Tooth cusp is a crucial structure, since the shape of the molar tooth is determined by number, shape, and size of the cusp. Bone morphogenetic protein (Bmp) signaling is known to play a critical role in tooth development, including in initiation. However, it remains unclear whether Bmp signaling is also involved in cusp formation. To address this question, we examined cusp in two different transge ...
DOI:
10.1016/j.gep.2019.04.002

13. Chrysanthemum requires short-day repeats for anthesis: Gradual CsFTL3 induction through a feedback loop under short-day conditions

Author:
Nakano, Yoshihiro; Takase, Tomoyuki; Takahashi, Shigekazu; Sumitomo, Katsuhiko; Higuchi, Yohei; Hisamatsu, Tamotsu
Source:
Plant science 2019 v.283 pp. 247-255
ISSN:
0168-9452
Subject:
gene overexpression,   etc   ; Chrysanthemum; RNA interference; flowering; leaves; loci; loss-of-function mutation; meristems; transgenes;     Show all 9 Subjects
Abstract:
... Chrysanthemums require continuous short-days (SD) for anthesis. FTL3 (FLOWERING LOCUS T-like 3), a floral promoter expressed in chrysanthemum leaf, forms a complex with its interacting partner FDL1 to induce floral meristem identity gene AFL1. We explored the FTL3 induction mechanism during SD repeats in Chrysanthemum seticuspe. CsFTL3 expression was not immediately induced by a shift from long-da ...
DOI:
10.1016/j.plantsci.2019.01.023

14. Effects of lipopolysaccharide structure on lycopene production in Escherichia coli

Author:
Cui, Mao; Wang, Zhou; Hu, Xiaoqing; Wang, Xiaoyuan
Source:
Enzyme and microbial technology 2019 v.124 pp. 9-16
ISSN:
0141-0229
Subject:
gene overexpression,   etc   ; Escherichia coli; bacteria; biosynthesis; lipopolysaccharides; lycopene; membrane permeability; mutants; operon;     Show all 9 Subjects
Abstract:
... Lipopolysaccharides, the major molecules in the outer membrane of Escherichia coli, affect the behavior of bacteria including outer membrane permeability, but its influence on lycopene production in E. coli has never been reported. In this study, the effects of lipopolysaccharides with different structures on lycopene biosynthesis were investigated. Firstly, the heterogenous crtEBI operon were ove ...
DOI:
10.1016/j.enzmictec.2019.01.009

15. Induction of S-nitrosoglutathione reductase reduces root cadmium uptake by inhibiting Iron-regulated transporter 1

Author:
Guan, Mei Yan; Zhu, Ya Xin; Liu, Xing Xing; Jin, Chong Wei
Source:
Plant and soil 2019 v.438 no.1-2 pp. 251-262
ISSN:
0032-079X
Subject:
gene overexpression,   etc   ; Arabidopsis; agricultural soils; cadmium; enzymes; mutants; mutation; polluted soils; roots; transgenic plants;     Show all 10 Subjects
Abstract:
... BACKGROUND AND AIMS: Increasing cadmium (Cd) contamination of agricultural soils is a serious problem. Identification of the mechanisms that control Cd uptake by roots is essential if we wish to improve the efficiency of plants in removing Cd from contaminated soils. METHODS: We dissected the role and the mechanism of S-nitrosoglutathione reductase (GSNOR) in regulating root Cd uptake in Arabidops ...
DOI:
10.1007/s11104-019-04014-z

16. Neural differentiation of mouse induced pluripotent stem cells using cadherin gene-engineered PA6 feeder cells

Author:
Paerhati, Paerwen; Ito, Akira; Yoshioka, Kantaro; Iwamoto, Kaori; Fujiwara, Sho; Horie, Masanobu; Kawabe, Yoshinori; Kamihira, Masamichi
Source:
Journal of bioscience and bioengineering 2019 v.127 no.5 pp. 633-640
ISSN:
1389-1723
Subject:
gene overexpression,   etc   ; cadherins; genes; mice; motor neurons; nerve regeneration; neurodevelopment; stem cells; stromal cells;     Show all 9 Subjects
Abstract:
... Investigating neural differentiation of pluripotent stem cells, including induced pluripotent stem (iPS) cells, is of importance for studying early neural development and providing a potential source of cells for nerve regeneration. Stromal cell-derived inducing activity (SDIA) using PA6 stromal cells promotes neural differentiation of iPS cells. Thus, we hypothesized that cadherin gene-engineered ...
DOI:
10.1016/j.jbiosc.2018.10.009

17. Autophagy exerts pivotal roles in regulatory effects of 1α,25-(OH)2D3 on the osteoclastogenesis

Author:
Ji, Lianmei; Gao, Jie; Kong, Ruina; Gao, Ying; Ji, Xiaofan; Zhao, Dongbao
Source:
Biochemical and biophysical research communications 2019 v.511 no.4 pp. 869-874
ISSN:
0006-291X
Subject:
gene overexpression,   etc   ; autophagy; bone marrow; cholecalciferol; drugs; genes; macrophages; osteoclasts; osteoporosis;     Show all 9 Subjects
Abstract:
... As an active form of vitamin D3, 1α,25-(OH)2D3 has a positive therapeutical effect on osteoporosis. However, 1α,25-(OH)2D3 can not only promote the osteoclastogenesis, but also inhibit the proliferation of osteoclast precursors (OCPs). Autophagy is regulated by 1α,25-(OH)2D3 and is considered to promote the osteoclastogenesis. Nevertheless, the role of 1α,25-(OH)2D3 in OCPs' autophagy remains unkn ...
DOI:
10.1016/j.bbrc.2019.02.114

18. Transcriptional regulation of the Ciona Gsx gene in the neural plate

Author:
Hudson, Clare; Esposito, Rosaria; Palladino, Antonio; Staiano, Leopoldo; Ferrier, David; Faure, Emmanuel; Lemaire, Patrick; Yasuo, Hitoyoshi; Spagnuolo, Antonietta
Source:
Developmental biology 2019 v.448 no.2 pp. 88-100
ISSN:
0012-1606
Subject:
gene overexpression,   etc   ; Ciona; brain; mitogen-activated protein kinase; models; signal transduction; transcription (genetics); transcription initiation site;     Show all 8 Subjects
Abstract:
... The ascidian neural plate consists of a defined number of identifiable cells organized in a grid of rows and columns, representing a useful model to investigate the molecular mechanisms controlling neural patterning in chordates. Distinct anterior brain lineages are specified via unique combinatorial inputs of signalling pathways with Nodal and Delta-Notch signals patterning along the medial-later ...
DOI:
10.1016/j.ydbio.2018.12.013

19. Cellular responses to proteostasis perturbations reveal non-optimal feedback in chaperone networks

Author:
Ghosh, Asmita; Gangadharan, Abhilash; Verma, Monika; Das, Sarada; Matai, Latika; Dash, Devi Prasanna; Dash, Debasis; Mapa, Koyeli; Chakraborty, Kausik
Source:
Cellular and molecular life sciences 2019 v.76 no.8 pp. 1605-1621
ISSN:
1420-682X
Subject:
gene overexpression,   etc   ; gene deletion; genes; protein folding; proteins; transcription (genetics); transcriptome;     Show all 7 Subjects
Abstract:
... The proteostasis network (PN) comprises a plethora of proteins that are dedicated to aid in protein folding and maintenance; some with overlapping functions. Despite this, there are multiple pathophysiological states associated with depletion of chaperones. This is counter-intuitive, assuming cells have the ability to re-program transcriptional outputs in accordance with its proteostasic limitatio ...
DOI:
10.1007/s00018-019-03013-8

20. Enhancement of gene knockdown efficiency by CNNC motifs in the intronic shRNA precursor

Author:
Park, Seong Kyun; Kee, Yun; Hwang, Byung Joon
Source:
Genes & genomics 2019 v.41 no.4 pp. 491-498
ISSN:
1976-9571
Subject:
gene overexpression,   etc   ; DNA-directed RNA polymerase; RNA interference; cytotoxicity; gene targeting; humans; microRNA; small interfering RNA;     Show all 8 Subjects
Abstract:
... BACKGROUND: Short hairpin RNAs (shRNAs) expressed from vectors have been used as an effective means of exploiting the RNA interference (RNAi) pathway in mammalian cells. Of several methods to express shRNA, a method of transcribing shRNAs embedded in microRNA precursors has been more widely used than the one that directly expresses shRNA from RNA polymerase III promoters because the microRNA precu ...
DOI:
10.1007/s13258-019-00783-0