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1. Genetic engineering of the acidophilic chemolithoautotroph Acidithiobacillus ferrooxidans

Author:
Heejung Jung; Yuta Inaba; Scott Banta
Source:
Trends in biotechnology 2022 v.40 no.6 pp. 677-692
ISSN:
0167-7799
Subject:
gene overexpression,   etc   ; Acidithiobacillus ferrooxidans; bioleaching; biotechnology; genes; iron; plasmids; sulfur;     Show all 8 Subjects
Abstract:
... There are several natural and anthropomorphic environments where iron- and/or sulfur-oxidizing bacteria thrive in extremely acidic conditions. These acidophilic chemolithautotrophs play important roles in biogeochemical iron and sulfur cycles, are critical catalysts for industrial metal bioleaching operations, and have underexplored potential in future biotechnological applications. However, their ...
DOI:
10.1016/j.tibtech.2021.10.004

2. Bone marrow mesenchymal stem cells-derived exosomal microRNA-16-5p restrains epithelial-mesenchymal transition in breast cancer cells via EPHA1/NF-κB signaling axis

Author:
Yuan Zhang; Xiaofeng Lai; Qingfang Yue; Fei Cao; Yue Zhang; Yang Sun; Jun Tian; Yizhao Lu; Li He; Jun Bai; Yifang Wei
Source:
Genomics 2022 v.114 no.3 pp. 110341
ISSN:
0888-7543
Subject:
gene overexpression,   etc   ; bone marrow; breast neoplasms; carcinogenesis; exosomes; genomics; mice; xenotransplantation;     Show all 8 Subjects
Abstract:
... This study intends to conquer the mystery of microRNA-16-5p/erythropoietin-producing hepatocellular A1/nuclear factor-κB signaling (miR-16-5p/EPHA1/NF-κB signaling) in breast cancer. Expression of miR-16-5p, EPHA1 and NF-κB signaling-related proteins were detected. Gene overexpression or silencing was used to examine the biological roles of bone marrow mesenchymal stem cells (BMSCs)-derived exo-mi ...
DOI:
10.1016/j.ygeno.2022.110341

3. A CRISPR/dCas9-based transcription activated system developed in marine microalga Nannochloropsis oceanica

Author:
Li Wei; Zhouyi Jiang; Bingqing Liu
Source:
Aquaculture 2022 v.546 pp. 737064
ISSN:
0044-8486
Subject:
gene overexpression,   etc   ; DNA; Nannochloropsis; RNA; RNA interference; aquaculture; carbon dioxide; feeds; genes; methylation; methyltransferases; microalgae; photosynthesis; transactivators; transcription (genetics); transcriptional activation;     Show all 16 Subjects
Abstract:
... Nannochloropsis is one of the most important microalgae utilized as the feedstuff in aquaculture and is emerging model oleaginous microalgae. Recently, different genetic tools such as RNA interference, gene overexpression and gene editing have been developed in Nannochloropsis. However, the available genetic modification methods based on CRISPR technology are rather limited for modulating gene exp ...
DOI:
10.1016/j.aquaculture.2021.737064

4. Improvement of Rimocidin Biosynthesis by Increasing Supply of Precursor Malonyl-CoA via Over-expression of Acetyl-CoA Carboxylase in Streptomyces rimosus M527

Author:
Zhijun Liao; Jinyao Zhang; Yue Shi; Yongyong Zhang; Zheng Ma; Andreas Bechthold; Xiaoping Yu
Source:
Current microbiology 2022 v.79 no.6 pp. 174
ISSN:
0343-8651
Subject:
gene overexpression,   etc   ; Streptomyces rimosus; acetyl-CoA carboxylase; biosynthesis; enzyme activity; fermentation; genes; macrolides; malonyl coenzyme A; microarray technology; secondary metabolites; time series analysis; transcriptome;     Show all 13 Subjects
Abstract:
... Precursor engineering is an effective strategy for the overproduction of secondary metabolites. The polyene macrolide rimocidin, which is produced by Streptomyces rimosus M527, exhibits a potent activity against a broad range of phytopathogenic fungi. It has been predicted that malonyl-CoA is used as extender units for rimocidin biosynthesis. Based on a systematic analysis of three sets of time-se ...
DOI:
10.1007/s00284-022-02867-9

5. The effect of 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase gene overexpression in the kynurenine pathway on the expression levels of indoleamine 2,3-dioxygenase 1 and interferon-γ in inflammatory conditions: an in vitro study

Author:
Marzieh Rostaminejad; Abdolmohamad Rostami; Abbas Behzad-Behbahani; Gholam Reza Rafiei Dehbidi; Tahereh Kalantari
Source:
Molecular biology reports 2022 v.49 no.2 pp. 1103-1111
ISSN:
0301-4851
Subject:
gene overexpression,   etc   ; cell lines; genes; genetic vectors; green fluorescent protein; indoleamine 2,3-dioxygenase; interferon-gamma; kynurenine pathway; lipopolysaccharides; macrophages; metabolites; mice; molecular biology; neurotoxicity; pathogenesis; plasmids; polyethyleneimine; quantitative polymerase chain reaction; transfection;     Show all 19 Subjects
Abstract:
... BACKGROUND: The kynurenine pathway (KP) can be involved in the pathogenesis of neurodegenerative diseases and excessive neurotoxic metabolite production. This study aimed to evaluate the effects of overexpression of murine 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase (Acmsd) gene in inflammatory conditions in RAW 264.7 cell line to present more information about the effect of this gene o ...
DOI:
10.1007/s11033-021-06935-4

6. Bacillus subtilis as a robust host for biochemical production utilizing biomass

Author:
Seo A. Park; Shashi Kant Bhatia; Hyun A. Park; Seo Yeong Kim; Pamidimarri D. V. N. Sudheer; Yung-Hun Yang; Kwon-Young Choi
Source:
Critical reviews in biotechnology 2021 v.41 no.6 pp. 827-848
ISSN:
1549-7801
Subject:
gene overexpression,   etc   ; Bacillus subtilis; N-acetylglucosamine; biomass; biotechnology; hyaluronic acid; oligopeptides; polyhydroxyalkanoates; protein secretion; xylooligosaccharides;     Show all 10 Subjects
Abstract:
... Bacillus subtilis is regarded as a suitable host for biochemical production owing to its excellent growth and bioresource utilization characteristics. In addition, the distinct endogenous metabolic pathways and the suitability of the heterologous pathways have made B. subtilis a robust and promising host for producing biochemicals, such as: bioalcohols; bioorganic acids (lactic acids, α-ketoglutar ...
DOI:
10.1080/07388551.2021.1888069

7. Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale

Author:
Yue Jian; Chenlu Zhang; Yating Wang; Zhiqing Li; Jing Chen; Wenting Zhou; Wenli Huang; Min Jiang; Hao Zheng; Mengyao Li; Huiying Miao; Fen Zhang; Huanxiu Li; Qiaomei Wang; Bo Sun
Source:
Genes 2021 v.12 no.8 pp. -
ISSN:
2073-4425
Subject:
gene overexpression,   etc   ; Brassica oleracea var. alboglabra; biosynthesis; chloroplasts; color; cultivars; flowers; genes; lutein; neoxanthin; violaxanthin;     Show all 11 Subjects
Abstract:
... Chinese kale (Brassica oleracea var. alboglabra) is rich in carotenoids, and neoxanthin is one of the most important carotenoids in Chinese kale. In this study, the function of the neoxanthin synthase gene (BoaNXS) in Chinese kale was investigated. BoaNXS, which had a 699-bp coding sequence, was cloned from the white flower cultivar of Chinese kale and was expressed in all developmental stages and ...
DOI:
10.3390/genes12081122

8. Effect of rosR Gene Overexpression on Biofilm Formation by Rhizobium leguminosarum

Author:
Z. R. Vershinina; O. V. Chubukova; Yu. M. Nikonorov; L. R. Khakimova; A. M. Lavina; L. R. Karimova; An. Kh. Baymiev; Al. Kh. Baymiev
Source:
Microbiology 2021 v.90 no.2 pp. 198-209
ISSN:
0026-2617
Subject:
gene overexpression,   etc   ; Rhizobium leguminosarum; biofilm; calcium; exopolysaccharides; genes; proteinases; regulatory proteins; root nodules; symbiosis; tomatoes;     Show all 11 Subjects
Abstract:
... The regulatory protein encoded by the rosR gene is involved in the processes of adaptation of root nodule bacteria Rhizobium leguminosarum to changes in environmental conditions. It affects the expression of a number of genes associated with the synthesis of exopolysaccharides, which play a critical role in formation of bacterial biofilms on various surfaces. The goal of this work was to study the ...
DOI:
10.1134/S0026261721020144

9. Cell death-inducing DNA fragmentation factor-α-like effector C (CIDEC) regulates acetate- and β-hydroxybutyrate-induced milk fat synthesis by increasing FASN expression in mammary epithelial cells of dairy cows

Author:
He Lv; Qingyu Meng; Nan Wang; Xiaoyu Duan; Xiaoming Hou; Ye Lin
Source:
Journal of dairy science 2021 v.104 no.5 pp. 6212-6221
ISSN:
0022-0302
Subject:
gene overexpression,   etc   ; DNA fragmentation; acetates; cell growth; epithelium; fatty-acid synthase; mammary glands; milk fat; transcription factors; triacylglycerols;     Show all 10 Subjects
Abstract:
... Increasing acetate and β-hydroxybutyrate (BHB) supply to lactating cows will increase milk fat synthesis. However, the underlying molecular mechanism remains largely unknown. Cell death-inducing DNA fragmentation factor-α-like effector C (CIDEC) is a lipid droplet-associated protein that promotes intracellular triacylglycerol accumulation. In the present study, using gene overexpression and knockd ...
DOI:
10.3168/jds.2020-18975

10. WUSCHEL Overexpression Promotes Callogenesis and Somatic Embryogenesis in Medicago truncatula Gaertn

Author:
Aline Kadri; Ghislaine Grenier De March; François Guerineau; Viviane Cosson; Pascal Ratet
Source:
Plants 2021 v.10 no.4 pp. -
ISSN:
2223-7747
Subject:
gene overexpression,   etc   ; Medicago truncatula; callus formation; genes; genetic engineering; jasmonic acid; leaves; plant growth; plant hormones; somatic embryogenesis;     Show all 10 Subjects
Abstract:
... The induction of plant somatic embryogenesis is often a limiting step for plant multiplication and genetic manipulation in numerous crops. It depends on multiple signaling developmental processes involving phytohormones and the induction of specific genes. The WUSCHEL gene (WUS) is required for the production of plant embryogenic stem cells. To explore a different approach to induce somatic embryo ...
DOI:
10.3390/plants10040715

11. Expression of the Cftr, Nfkb1, and Ocln Genes during Restoration of Skin Integrity

Author:
A. S. Huet; K. O. Dvorshchenko; D. M. Grebinyk; T. V. Beregova; L. I. Ostapchenko
Source:
Cytology and genetics 2022 v.56 no.3 pp. 236-243
ISSN:
0095-4527
Subject:
gene overexpression,   etc   ; cell biology; genes; melanin;     Show all 4 Subjects
Abstract:
... The expression level of the Cftr and Ocln genes was shown to decrease against an increase in the Nfkb1 gene expression level during the healing of both planar full-thickness excisional and purulonecrotic skin wounds in rats. The restoration of the Ocln gene expression could be mediated by an increase in the Cftr gene expression level, due to a decrease in the mRNA level of the Nfkb1 gene. With the ...
DOI:
10.3103/S0095452722030148

12. Cloning of the ben gene and its functional identification in Cordyceps militaris

Author:
Hai-Wei Lou; Yu Zhao; Chuan-Shun Ren; Ren-Yong Zhao; Zhi-Wei Ye; Jun-Fang Lin; Li-Qiong Guo
Source:
Scientia horticulturae 2021 v.281 pp. 109953
ISSN:
0304-4238
Subject:
gene overexpression,   etc   ; Agrobacterium radiobacter; Cordyceps militaris; Neurospora crassa; benomyl; biosynthesis; fungicides; gene targeting; genetic markers; homologous recombination; plasmids; resistance genes;     Show all 12 Subjects
Abstract:
... Selectable marker genes are very important for the screening of target mutants. At least two selectable marker genes are usually required to identify the function of a gene by gene knockout, gene complementation, and gene overexpression. However, there are few selectable marker genes that can be applied to Cordyceps militaris. In order to obtain the selectable marker gene which can be used to C. m ...
DOI:
10.1016/j.scienta.2021.109953

13. Increasing NADPH Availability for Xylitol Production via Pentose-Phosphate-Pathway Gene Overexpression and Embden–Meyerhof–Parnas-Pathway Gene Deletion in Escherichia coli

Author:
Xinsong Yuan; Yudi Mao; Shuai Tu; Jianping Lin; Huahao Shen; Lirong Yang; Mianbin Wu
Source:
Journal of agricultural and food chemistry 2021 v.69 no.33 pp. 9625-9631
ISSN:
1520-5118
Subject:
gene overexpression,   etc   ; Escherichia coli; batch fermentation; bioreactors; biotransformation; corn cobs; food chemistry; gene deletion; glucose; glycolysis; pentose phosphate cycle; xylitol; xylose;     Show all 13 Subjects
Abstract:
... Cofactor availability is often a rate-limiting factor in the bioconversion of xylose to xylitol. The overexpression of pentose phosphate pathway genes and the deletion of Embden–Meyerhof–Parnas pathway genes can modulate the glucose metabolic flux and increase the intracellular NADPH supply, enabling Escherichia coli cells to produce xylitol from corncob hydrolysates. The effects of zwf and/or gnd ...
DOI:
10.1021/acs.jafc.1c03283

14. Promoter hypomethylation as potential confounder of Ras gene overexpression and their clinical significance in subsets of urothelial carcinoma of bladder

Author:
Kiran Tripathi; Apul Goel; Atin Singhai; Minal Garg
Source:
Molecular biology reports 2021 v.48 no.3 pp. 2183-2199
ISSN:
0301-4851
Subject:
gene overexpression,   etc   ; bladder; carcinoma; demographic statistics; genes; genomic islands; methylation; molecular biology; muscles; pathogenesis; prognosis; rats; sarcoma; transcriptomics; urinary bladder neoplasms;     Show all 15 Subjects
Abstract:
... Overexpression of normal Ras and its aberrant CpG island methylation in the promoter regions have been shown to direct cells for uncontrolled abnormal growth and bladder tumor formation and therefore, fetched recent attention as a marker of diagnosis and prognosis to predict the biological behavior of urothelial carcinoma of bladder (UCB). Methylation pattern at CpG islands of the promoter regions ...
DOI:
10.1007/s11033-021-06227-x

15. Inhibition of hepatocyte nuclear factor 4 confers imidacloprid resistance in Nilaparvata lugens via the activation of cytochrome P450 and UDP-glycosyltransferase genes

Author:
Yibei Cheng; Yimin Li; Wenru Li; Yuanyuan Song; Rensen Zeng; Kai Lu
Source:
Chemosphere 2021 v.263 pp. 128269
ISSN:
0045-6535
Subject:
gene overexpression,   etc   ; Nilaparvata lugens; RNA interference; cytochrome P-450; gene activation; genetic resistance; glycosyltransferases; imidacloprid; insecticide resistance; metabolic detoxification; transcription factors; transcriptomics;     Show all 12 Subjects
Abstract:
... Increasing evidence indicates that insect resistance to synthesized insecticides is regulated by the nuclear receptors. However, the underlying mechanisms of this regulation are not clear. Here, we demonstrate that inhibition of hepatocyte nuclear factor 4 (HNF4) confers imidacloprid resistance in the brown planthopper (BPH) Nilaparvata lugens by regulating cytochrome P450 and UDP-glycosyltransfer ...
DOI:
10.1016/j.chemosphere.2020.128269

16. Two Tomato (Solanum lycopersicum) Thaumatin-Like Protein Genes Confer Enhanced Resistance to Late Blight (Phytophthora infestans)

Author:
Haishan Zhu; Minghua Deng; Zhengan Yang; Lianzhen Mao; Shurui Jiang; Yanling Yue; Kai Zhao
Source:
Phytopathology 2021 v.111 no.10 pp. 1790-1799
ISSN:
0031-949X
Subject:
gene overexpression,   etc   ; Phytophthora infestans; Solanum lycopersicum; cluster analysis; disease resistance; gene expression regulation; plant pathology; quantitative polymerase chain reaction; resistance genes; tomatoes; transcriptome;     Show all 11 Subjects
Abstract:
... Late blight (caused by Phytophthora infestans) poses a serious threat to tomato production but the number of late blight resistance genes isolated from tomato is limited, making resistance gene mining a high research priority. In this study, highly resistant CLN2037E and susceptible No. 5 tomato inbred lines were used to identify late blight resistance genes. Using transcriptome sequencing, we dis ...
DOI:
10.1094/PHYTO-06-20-0237-R

17. Tlr2 and Tjp1 Genes’ Expression during Restoration of Skin Integrity

Author:
A. Huet; K. Dvorshchenko; O. Taburets; D. Grebinyk; T. Beregova; L. Ostapchenko
Source:
Cytology and genetics 2020 v.54 no.6 pp. 539-545
ISSN:
0095-4527
Subject:
gene overexpression,   etc   ; cell biology; free radicals; genes; melanin; superoxide anion;     Show all 6 Subjects
Abstract:
... Decrease in Tjp 1 gene expression against the background of the activation of free radical processes (increase in the content of superoxide anion-radical) was observed during full-thickness skin wounds' healing in rats. The restoration of the expression level of this gene may be mediated by an increase in the expression level of the Tlr2 gene. The level of Tjp1 gene expression, as well as the cont ...
DOI:
10.3103/S0095452720060122

18. Bombyx mori cell division cycle protein 37 promotes the proliferation of BmNPV

Author:
Qian Zhang; Yun-Fei Wu; Peng Chen; Tai-Hang Liu; Zhan-Qi Dong; Cheng Lu; Min-Hui Pan
Source:
Pesticide biochemistry and physiology 2021 v.178 pp. 104923
ISSN:
0048-3575
Subject:
gene overexpression,   etc   ; Bombyx mori; Bombyx mori nucleopolyhedrovirus; cell division; enzyme activation; gene expression regulation; genes; heat-shock protein 90; insect proteins; silkworms; virus replication;     Show all 11 Subjects
Abstract:
... Cell division cycle protein 37 (Cdc37) is a molecular chaperone that actively participates in many intracellular physiological and biochemical processes as well as pathogen infection. However, the function of Cdc37 in silkworm cells under Bombyx mori nucleopolyhedrovirus (BmNPV) infection is unknown. We cloned and identified BmCdc37, a Cdc37 gene from B. mori, which is highly conserved among other ...
DOI:
10.1016/j.pestbp.2021.104923

19. Potential of three local marine microalgae from Tunisian coasts for cadmium, lead and chromium removals

Author:
Jihen Elleuch; Rihab Hmani; Marwa Drira; Philippe Michaud; Imen Fendri; Slim Abdelkafi
Source:
Science of the total environment 2021 v.799 pp. 149464
ISSN:
0048-9697
Subject:
gene overexpression,   etc   ; Amphora; Dunaliella salina; Navicula; antioxidants; bioaccumulation; bioremediation; cadmium; catalase; chelating agents; chromium; cost effectiveness; environment; genes; lead; microalgae; phosphates; sulfates; superoxide dismutase; water pollution;     Show all 20 Subjects
Abstract:
... Metal elements are widely used in various industrial activities and are considered as common water source contaminants. Thus, the development of cost-effective, simple design and efficient processes for trace metal elements removal from contaminated water sources is of great interest. The effects of cadmium, lead and chromium on growth, biomolecules accumulation and metabolic responses of Amphora ...
DOI:
10.1016/j.scitotenv.2021.149464

20. Functional identification of apple MdMYB2 gene in phosphate-starvation response

Author:
Yu-Ying Yang; Yi-Ran Ren; Peng-Fei Zheng; Feng-Jia Qu; Lai-Qing Song; Chun-Xiang You; Xiao-Fei Wang; Yu-Jin Hao
Source:
Journal of plant physiology 2020 v.244 pp. 153089
ISSN:
0176-1617
Subject:
gene overexpression,   etc   ; Arabidopsis; apples; flowering; genes; gibberellic acid; phosphates; plant growth; starvation;     Show all 9 Subjects
Abstract:
... Inorganic phosphate (Pi) starvation severely affects the normal growth and development of plants. Here, a Pi-responsive gene, named MdMYB2 (MDP0000823458), was cloned and functionally identified in apple. Overexpression of MdMYB2 regulated the expression of Pi starvation-induced (PSI) genes and then promoted phosphate assimilation and utilization. The ectopic expression of MdMYB2 in Arabidopsis in ...
DOI:
10.1016/j.jplph.2019.153089