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1. Cloning and Characterization of phzR Gene from Pseudomonas aeruginosa

Author:
Pang, Bingbing; Liu, Tingting; Zhang, Wenjia; Ye, Fengcai; Shang, Changhua
Source:
Current microbiology 2021 v.78 no.4 pp. 1482-1487
ISSN:
0343-8651
Subject:
gene overexpression,   etc   ; Pseudomonas aeruginosa; amino acid sequences; bacteria; genes; promoter regions;     Show all 6 Subjects
Abstract:
... A gene encoding phzR was isolated from a phenazine-producing bacterium Pseudomonas aeruginosa 2016NX1. This paper provided the full-length cDNA encoding phzR (GenBank Accession no., MW143078). The cDNA of the phzR contained an open reading frame (ORF) of 714 bp. The potential regulatory elements were predicted in the phzR promoter region. The deduced amino acid sequence of P. aeruginosa phzR showe ...
DOI:
10.1007/s00284-021-02410-2

2. Cell death-inducing DNA fragmentation factor-α-like effector C (CIDEC) regulates acetate- and β-hydroxybutyrate-induced milk fat synthesis by increasing FASN expression in mammary epithelial cells of dairy cows

Author:
Lv, He; Meng, Qingyu; Wang, Nan; Duan, Xiaoyu; Hou, Xiaoming; Lin, Ye
Source:
Journal of dairy science 2021 v.104 no.5 pp. 6212-6221
ISSN:
0022-0302
Subject:
gene overexpression,   etc   ; DNA fragmentation; acetates; cell growth; epithelium; fatty-acid synthase; mammary glands; milk fat; transcription factors; triacylglycerols;     Show all 10 Subjects
Abstract:
... Increasing acetate and β-hydroxybutyrate (BHB) supply to lactating cows will increase milk fat synthesis. However, the underlying molecular mechanism remains largely unknown. Cell death-inducing DNA fragmentation factor-α-like effector C (CIDEC) is a lipid droplet-associated protein that promotes intracellular triacylglycerol accumulation. In the present study, using gene overexpression and knockd ...
DOI:
10.3168/jds.2020-18975

3. Penicilliumdigitatum MFS transporters can display different roles during pathogen-fruit interaction

Author:
de Ramón-Carbonell, Marta; Sánchez-Torres, Paloma
Source:
International journal of food microbiology 2021 v.337 pp. 108918
ISSN:
0168-1605
Subject:
gene overexpression,   etc   ; Penicillium digitatum; citrus fruits; food microbiology; fruits; fungi; fungicide resistance; fungicides; genes; transcription (genetics); virulence;     Show all 11 Subjects
Abstract:
... Major facilitator superfamily (MFS) comprises a large family of fungal transporters. In this work four Penicillium digitatum MFS transporters named PdMFS2–5 were identified and functionally characterized through gene elimination and gene overexpression with aim of unveil the similarities and differences among members of the same family during pathogen-fruit interaction. Fungal mutants in which eac ...
DOI:
10.1016/j.ijfoodmicro.2020.108918

4. Coordination between GROWTH-REGULATING FACTOR1 and GRF-INTERACTING FACTOR1 plays a key role in regulating leaf growth in rice

Author:
Lu, Yuzhu; Meng, Yunlong; Zeng, Jia; Luo, Ying; Feng, Zhen; Bian, Liying; Gao, Suyun
Source:
BMC plant biology 2020 v.20 no.1 pp. 200
ISSN:
1471-2229
Subject:
gene overexpression,   etc   ; apical meristems; cell proliferation; gibberellins; leaves; phenotype; rice;     Show all 7 Subjects
Abstract:
... BACKGROUND: The interactions between Growth-regulating factors (GRFs) and GRF-Interacting Factors (GIFs) have been well demonstrated but it remains unclear whether different combinations of GRF and GIF play distinctive roles in the pathway downstream of the complex. RESULTS: Here we showed that OsGRF1 and OsGIF1 synergistically regulate leaf growth in rice. The expression of OsGIF1 emerged in all ...
DOI:
10.1186/s12870-020-02417-0

5. Effects of HSP27 gene expression on the resistance to Escherichia coli infection in piglets

Author:
Zong, Qiufang.; Jing, Pengfei; Sun, Shouyong; Wang, Haifei; Wu, Shenglong; Bao, Wenbin
Source:
Gene 2021 v.773 pp. 145415
ISSN:
0378-1119
Subject:
gene overexpression,   etc   ; Escherichia coli; Escherichia infections; adhesion; cells; gene expression regulation; genes; heat shock proteins; immune response; infection; inflammation; piglets; quantitative polymerase chain reaction; secretion; thallus; tissues;     Show all 16 Subjects
Abstract:
... Heat shock protein 27 (HSP27) plays an important role in protecting cells from various stress factors. This study aimed to investigate the function of HSP27 gene and its regulatory mechanism as infected by Escherichia coli (E. coli) at the tissue and cellular levels. Real-time PCR was used to detect the differential expression of HSP27 gene in F18 resistant and sensitive Sutai pigs and the differe ...
DOI:
10.1016/j.gene.2021.145415

6. Targeted delivery of siRNAs against hepatocellular carcinoma-related genes by a galactosylated polyaspartamide copolymer

Author:
Perrone, Francesca; Craparo, Emanuela Fabiola; Cemazar, Maja; Kamensek, Urska; Drago, Salvatore Emanuele; Dapas, Barbara; Scaggiante, Bruna; Zanconati, Fabrizio; Bonazza, Debora; Grassi, Mario; Truong, Nhung; Pozzato, Gabriele; Farra, Rossella; Cavallaro, Gennara; Grassi, Gabriele
Source:
Journal of controlled release 2021 v.330 pp. 1132-1151
ISSN:
0168-3659
Subject:
gene overexpression,   etc   ; animal models; composite polymers; galactose; gene expression regulation; genes; green fluorescent protein; hepatocytes; hepatoma; humans; messenger RNA; mice; polyethylene glycol; small interfering RNA; toxicity; xenotransplantation;     Show all 16 Subjects
Abstract:
... Given the lack of effective treatments for Hepatocellular carcinoma (HCC), the development of novel therapeutic approaches is very urgent. Here, siRNAs were delivered to HCC cells by a synthetic polymer containing α,β-poly-(N-2-hydroxyethyl)-D,L-aspartamide-(PHEA) derivatized with diethylene triamine (DETA) and bearing in the side chain galactose (GAL) linked via a polyethylene glycol (PEG) to obt ...
DOI:
10.1016/j.jconrel.2020.11.020

7. OsVP1 activates Sdr4 expression to control rice seed dormancy via the ABA signaling pathway

Author:
Chen, Wenqiang; Wang, Wei; Lyu, Yusong; Wu, Yawen; Huang, Pingliang; Hu, Shikai; Wei, Xiangjin; Jiao, Guiai; Sheng, Zhonghua; Tang, Shaoqing; Shao, Gaoneng; Luo, Ju
Source:
The crop journal 2021 v.9 no.1 pp. 68-78
ISSN:
2214-5141
Subject:
gene overexpression,   etc   ; Oryza sativa; abscisic acid; financial economics; gene editing; genes; loss-of-function mutation; mutants; protoplasts; rice; seed dormancy; seed germination; signal transduction; sprouting; vivipary (plants);     Show all 15 Subjects
Abstract:
... Pre-harvest sprouting (PHS) is a disadvantageous trait in cereal production worldwide, causing large economic losses each year. Its regulation mechanism is still unclear. We generated the Oryza sativa Viviparous1 (OsVP1) mutant using gene editing technique, which shows increased PHS compared with that of the wild type Nipponbare. OsVP1 is localized mainly in the nucleus and expressed in various ti ...
DOI:
10.1016/j.cj.2020.06.005

8. Overexpression of IbPAL1 promotes chlorogenic acid biosynthesis in sweetpotato

Author:
Yu, Yang; Wang, Yingjie; Yu, Yue; Ma, Peiyong; Jia, Zhaodong; Guo, Xiaoding; Xie, Yizhi; Bian, Xiaofeng
Source:
The crop journal 2021 v.9 no.1 pp. 204-215
ISSN:
2214-5141
Subject:
gene overexpression,   etc   ; Ipomoea batatas; bioactive properties; biochemical pathways; biosynthesis; chlorogenic acid; food crops; food security; health promotion; leaves; phenylalanine ammonia-lyase; polyphenols; secondary xylem; stems; sweet potatoes;     Show all 15 Subjects
Abstract:
... Sweetpotato [Ipomoea batatas (L.) Lam.], a food crop with both nutritional and medicinal uses, plays essential roles in food security and health-promoting. Chlorogenic acid (CGA), a polyphenol displaying several bioactivities, is distributed in all edible parts of sweetpotato. However, little is known about the specific metabolism of CGA in sweetpotato. In this study, IbPAL1, which encodes an endo ...
DOI:
10.1016/j.cj.2020.06.003

9. Nitroreductase Activites in Giardia lamblia: ORF 17150 Encodes a Quinone Reductase with Nitroreductase Activity

Author:
Müller, Joachim; Heller, Manfred; Uldry, Anne-Christine; Braga, Sophie; Müller, Norbert
Source:
Pathogens 2021 v.10 no.2
ISSN:
2076-0817
Subject:
gene overexpression,   etc   ; Giardia lamblia; NAD(P)H dehydrogenase (quinone); enzyme activity; flavoproteins; gel chromatography; mass spectrometry; metronidazole; nitroreductases; open reading frames; recombinant proteins;     Show all 11 Subjects
Abstract:
... The intestinal diplomonadid Giardia lamblia is a causative agent of persistent diarrhea. Current treatments are based on nitro drugs, especially metronidazole. Nitro compounds are activated by reduction, yielding toxic intermediates. The enzymatic systems responsible for this activation are not completely understood. By fractionating cell free crude extracts by size exclusion chromatography follow ...
DOI:
10.3390/pathogens10020129

10. Inhibition of hepatocyte nuclear factor 4 confers imidacloprid resistance in Nilaparvata lugens via the activation of cytochrome P450 and UDP-glycosyltransferase genes

Author:
Cheng, Yibei; Li, Yimin; Li, Wenru; Song, Yuanyuan; Zeng, Rensen; Lu, Kai
Source:
Chemosphere 2021 v.263 pp. 128269
ISSN:
0045-6535
Subject:
gene overexpression,   etc   ; Nilaparvata lugens; RNA interference; cytochrome P-450; gene activation; genetic resistance; glycosyltransferases; imidacloprid; insecticide resistance; metabolic detoxification; transcription factors; transcriptomics;     Show all 12 Subjects
Abstract:
... Increasing evidence indicates that insect resistance to synthesized insecticides is regulated by the nuclear receptors. However, the underlying mechanisms of this regulation are not clear. Here, we demonstrate that inhibition of hepatocyte nuclear factor 4 (HNF4) confers imidacloprid resistance in the brown planthopper (BPH) Nilaparvata lugens by regulating cytochrome P450 and UDP-glycosyltransfer ...
DOI:
10.1016/j.chemosphere.2020.128269

11. Correction to: Gene overexpression screen for chromosome instability in yeast primarily identifies cell cycle progression genes

Author:
Tutaj, Hanna; Pogoda, Elzbieta; Tomala, Katarzyna; Korona, Ryszard
Source:
Current genetics 2019 v.65 no.2 pp. 493
ISSN:
0172-8083
Subject:
gene overexpression,   etc   ; cell cycle; chromosomes; genes; yeasts;     Show all 5 Subjects
Abstract:
... In the original publication, ‘Frumkin JP et al.’ reference was missed to include in the reference list. The complete reference should read as below. ...
DOI:
10.1007/s00294-018-0893-x
PubMed:
30327879
PubMed Central:
PMC6828142

12. Corrections to: Enhanced lincomycin production by co‑overexpression of metK1 and metK2 in Streptomyces lincolnensis

Author:
Xu, Yurong; Tan, Guoqing; Ke, Meilan; Li, Jie; Tang, Yaqian; Meng, Sitong; Niu, Jingjing; Wang, Yansheng; Liu, Ruihua; Wu, Hang; Bai, Linquan; Zhang, Lixin; Zhang, Buchang
Source:
Journal of industrial microbiology & biotechnology 2018 v.45 no.6 pp. 447-448
ISSN:
1367-5435
Subject:
gene overexpression,   etc   ; Streptomyces lincolnensis; lincomycin;     Show all 3 Subjects
Abstract:
... In the online published article, row value “pIB139-metK1-metK2” in table 1 has been processed incorrectly. The correct table is given below ...
DOI:
10.1007/s10295-018-2036-2

13. Chassis engineering for microbial production of chemicals: from natural microbes to synthetic organisms

Author:
Liu, Jingyi; Wu, Xia; Yao, Mingdong; Xiao, Wenhai; Zha, Jian
Source:
Current opinion in biotechnology 2020 v.66 pp. 105-112
ISSN:
0958-1669
Subject:
gene overexpression,   etc   ; artificial cells; biobased products; biosynthesis; gene deletion; genes; manufacturing; microorganisms; synthetic biology;     Show all 9 Subjects
Abstract:
... Chassis provides a setting for the expression of heterologous pathway genes, which often requires extensive engineering to achieve complete functions. Traditionally, chassis engineering relies on gene deletion/overexpression for the regulation of precursor/cofactor supply and product transportation, which has generated thousands of high-performance strains. With the development of synthetic biolog ...
DOI:
10.1016/j.copbio.2020.06.013

14. Dysregulation of cancer genes by recurrent intergenic fusions

Author:
Yun, Jae Won; Yang, Lixing; Park, Hye-Young; Lee, Chang-Woo; Cha, Hongui; Shin, Hyun-Tae; Noh, Ka-Won; Choi, Yoon-La; Park, Woong-Yang; Park, Peter J.
Source:
Genome biology 2020 v.21 no.1 pp. 166
ISSN:
1474-760X
Subject:
gene overexpression,   etc   ; breasts; carcinogenesis; colon; genes; messenger RNA; patients; sequence analysis; therapeutics; thyroid neoplasms;     Show all 10 Subjects
Abstract:
... BACKGROUND: Gene fusions have been studied extensively, as frequent drivers of tumorigenesis as well as potential therapeutic targets. In many well-known cases, breakpoints occur at two intragenic positions, leading to in-frame gene-gene fusions that generate chimeric mRNAs. However, fusions often occur with intergenic breakpoints, and the role of such fusions has not been carefully examined. RESU ...
DOI:
10.1186/s13059-020-02076-2

15. Global identification and analysis revealed differentially expressed lncRNAs associated with meiosis and low fertility in autotetraploid rice

Author:
Li, Xiang; Shahid, Muhammad Qasim; Wen, Minsi; Chen, Shuling; Yu, Hang; Jiao, Yamin; Lu, Zijun; Li, Yajing; Liu, Xiangdong
Source:
BMC plant biology 2020 v.20 no.1 pp. 82
ISSN:
1471-2229
Subject:
gene overexpression,   etc   ; CRISPR-Cas systems; autotetraploidy; embryo sac; germplasm; megasporocytes; meiosis; mutants; pollen; rice; sequence analysis;     Show all 11 Subjects
Abstract:
... BACKGROUND: Autotetraploid rice is a useful germplasm for polyploid rice breeding. Our previous research showed that non-coding RNAs might be associated with low fertility in autotetraploid rice. However, little information is available on long non-coding RNAs (lncRNAs) involved in the low fertility of autotetraploid rice. In the present study, RNA-seq was employed to detect the differentially exp ...
DOI:
10.1186/s12870-020-2290-0

16. miR-29a-3p suppresses hepatic fibrosis pathogenesis by modulating hepatic stellate cell proliferation via targeting PIK3R3 gene expression

Author:
Fu, Juan; Wu, Biao; Zhong, Shaohua; Deng, Wei; Lin, Feng
Source:
Biochemical and biophysical research communications 2020 v.529 no.4 pp. 922-929
ISSN:
0006-291X
Subject:
gene overexpression,   etc   ; Western blotting; cell proliferation; genes; liver cirrhosis; luciferase; microRNA; models; pathogenesis; phosphorylation; research; transfection;     Show all 12 Subjects
Abstract:
... Hepatic stellate cells (HSC) activation and proliferation mediated the pathogenic development of hepatic fibrosis (HF). However, the underlying mechanisms remain poorly understood. In this study, we aimed to investigate the miR-29a-3p and its effects on PIK3R3 expression in HF pathogenesis.LX-2 cells treated with TGF-β1 was used as the in vitro HF model. The expression of microRNAs and proteins in ...
DOI:
10.1016/j.bbrc.2020.06.102

17. ESCRT-I Component VPS23A Sustains Salt Tolerance by Strengthening the SOS Module in Arabidopsis

Author:
Lou, Lijuan; Yu, Feifei; Tian, Miaomiao; Liu, Guangchao; Wu, Yaorong; Wu, Yujiao; Xia, Ran; Pardo, Jose M.; Guo, Yan; Xie, Qi
Source:
Molecular plant 2020 v.13 no.8 pp. 1134-1148
ISSN:
1674-2052
Subject:
gene overexpression,   etc   ; Arabidopsis; antiporters; excretion; plasma membrane; regulatory proteins; salt stress; salt tolerance; seedlings; sodium;     Show all 10 Subjects
Abstract:
... The Salt-Overly-Sensitive (SOS) signaling module, comprising the sodium-transport protein SOS1 and the regulatory proteins SOS2 and SOS3, is well known as the central salt excretion system, which helps protect plants against salt stress. Here we report that VPS23A, a component of the ESCRT (endosomal sorting complex required for transport), plays an essential role in the function of the SOS module ...
DOI:
10.1016/j.molp.2020.05.010

18. Alpinetin Inhibits Oral Squamous Cell Carcinoma Proliferation via miR-211-5p Upregulation and Notch Pathway Deactivation

Author:
Guo, Yanjun; Chen, Yong; Liu, Hongli; Yan, Wei
Source:
Nutrition and cancer 2020 v.72 no.5 pp. 757-767
ISSN:
1532-7914
Subject:
gene overexpression,   etc   ; cell viability; cyclins; human cell lines; interphase; mice; models; plasmids; squamous cell carcinoma;     Show all 9 Subjects
Abstract:
... Background: The effect of alpinetin (ALP) on miR-211-5p level and function in oral squamous cell carcinoma (OSCC) remains unclear. Materials and methods: Human OSCC cell lines (CAL-27 and TCA-8113) and a mouse xenograft model with subcutaneously injected TCA-8113 cells were used. Effect of ALP treatment on cell viability, cell cycle distributions, and p-p53, p21, c-PARP, cyclin D1, NICD, HES1, and ...
DOI:
10.1080/01635581.2019.1651878

19. miRNA target identification and prediction as a function of time in gene expression data

Author:
Grigaitis, Pranas; Starkuviene, Vytaute; Rost, Ursula; Serva, Andrius; Pucholt, Pascal; Kummer, Ursula
Source:
RNA biology 2020 v.17 no.7 pp. 990-1000
ISSN:
1555-8584
Subject:
gene overexpression,   etc   ; algorithms; bioinformatics; genes; humans; microRNA; microarray technology; prediction; screening;     Show all 9 Subjects
Abstract:
... The understanding of miRNA target interactions is still limited due to conflicting data and the fact that high-quality validation of targets is a time-consuming process. Faster methods like high-throughput screens and bioinformatics predictions are employed but suffer from several problems. One of these, namely the potential occurrence of downstream (i.e. secondary) effects in high-throughput scre ...
DOI:
10.1080/15476286.2020.1748921

20. Effects on the sporulation and secondary metabolism yields of Monascus purpureus with mokH gene deletion and overexpression

Author:
Zhang, Chan; Zhu, Qianqian; Zhang, Han; Zhang, Nan; Yang, Xuelian; Shi, Jiachen; Sun, Baoguo; Wang, Chengtao
Source:
Fungal biology 2020 v.124 no.7 pp. 661-670
ISSN:
1878-6146
Subject:
gene overexpression,   etc   ; Monascus purpureus; biosynthesis; cholesterol; gene deletion; humans; lovastatin; mycelium; secondary metabolites; sporulation;     Show all 10 Subjects
Abstract:
... Monacolin K is a secondary metabolite of Monascus and is known to decrease cholesterol levels in humans. There are 9 genes (mokA-mokI) controlling its biosynthesis, of which mokH is thought to act as a pathway-specific regulator. In this study, the Monascus purpureus M1 strain was compared with mokH gene deletion strains (△H1) and overexpression strains (H7). The monacolin K yields in the △H1 stra ...
DOI:
10.1016/j.funbio.2020.03.010