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geneoverexpression, etc ; Acidithiobacillus ferrooxidans; bioleaching; biotechnology; genes; iron; plasmids; sulfur; Show all 8 Subjects
Abstract:
... There are several natural and anthropomorphic environments where iron- and/or sulfur-oxidizing bacteria thrive in extremely acidic conditions. These acidophilic chemolithautotrophs play important roles in biogeochemical iron and sulfur cycles, are critical catalysts for industrial metal bioleaching operations, and have underexplored potential in future biotechnological applications. However, their ...
geneoverexpression, etc ; bone marrow; breast neoplasms; carcinogenesis; exosomes; genomics; mice; xenotransplantation; Show all 8 Subjects
Abstract:
... This study intends to conquer the mystery of microRNA-16-5p/erythropoietin-producing hepatocellular A1/nuclear factor-κB signaling (miR-16-5p/EPHA1/NF-κB signaling) in breast cancer. Expression of miR-16-5p, EPHA1 and NF-κB signaling-related proteins were detected. Gene overexpression or silencing was used to examine the biological roles of bone marrow mesenchymal stem cells (BMSCs)-derived exo-mi ...
... Nannochloropsis is one of the most important microalgae utilized as the feedstuff in aquaculture and is emerging model oleaginous microalgae. Recently, different genetic tools such as RNA interference, gene overexpression and gene editing have been developed in Nannochloropsis. However, the available genetic modification methods based on CRISPR technology are rather limited for modulating gene exp ...
geneoverexpression, etc ; Streptomyces rimosus; acetyl-CoA carboxylase; biosynthesis; enzyme activity; fermentation; genes; macrolides; malonyl coenzyme A; microarray technology; secondary metabolites; time series analysis; transcriptome; Show all 13 Subjects
Abstract:
... Precursor engineering is an effective strategy for the overproduction of secondary metabolites. The polyene macrolide rimocidin, which is produced by Streptomyces rimosus M527, exhibits a potent activity against a broad range of phytopathogenic fungi. It has been predicted that malonyl-CoA is used as extender units for rimocidin biosynthesis. Based on a systematic analysis of three sets of time-se ...
... BACKGROUND: The kynurenine pathway (KP) can be involved in the pathogenesis of neurodegenerative diseases and excessive neurotoxic metabolite production. This study aimed to evaluate the effects of overexpression of murine 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase (Acmsd) gene in inflammatory conditions in RAW 264.7 cell line to present more information about the effect of this gene o ...
geneoverexpression, etc ; Bacillus subtilis; N-acetylglucosamine; biomass; biotechnology; hyaluronic acid; oligopeptides; polyhydroxyalkanoates; protein secretion; xylooligosaccharides; Show all 10 Subjects
Abstract:
... Bacillus subtilis is regarded as a suitable host for biochemical production owing to its excellent growth and bioresource utilization characteristics. In addition, the distinct endogenous metabolic pathways and the suitability of the heterologous pathways have made B. subtilis a robust and promising host for producing biochemicals, such as: bioalcohols; bioorganic acids (lactic acids, α-ketoglutar ...
geneoverexpression, etc ; Brassica oleracea var. alboglabra; biosynthesis; chloroplasts; color; cultivars; flowers; genes; lutein; neoxanthin; violaxanthin; Show all 11 Subjects
Abstract:
... Chinese kale (Brassica oleracea var. alboglabra) is rich in carotenoids, and neoxanthin is one of the most important carotenoids in Chinese kale. In this study, the function of the neoxanthin synthase gene (BoaNXS) in Chinese kale was investigated. BoaNXS, which had a 699-bp coding sequence, was cloned from the white flower cultivar of Chinese kale and was expressed in all developmental stages and ...
geneoverexpression, etc ; Rhizobium leguminosarum; biofilm; calcium; exopolysaccharides; genes; proteinases; regulatory proteins; root nodules; symbiosis; tomatoes; Show all 11 Subjects
Abstract:
... The regulatory protein encoded by the rosR gene is involved in the processes of adaptation of root nodule bacteria Rhizobium leguminosarum to changes in environmental conditions. It affects the expression of a number of genes associated with the synthesis of exopolysaccharides, which play a critical role in formation of bacterial biofilms on various surfaces. The goal of this work was to study the ...
geneoverexpression, etc ; DNA fragmentation; acetates; cell growth; epithelium; fatty-acid synthase; mammary glands; milk fat; transcription factors; triacylglycerols; Show all 10 Subjects
Abstract:
... Increasing acetate and β-hydroxybutyrate (BHB) supply to lactating cows will increase milk fat synthesis. However, the underlying molecular mechanism remains largely unknown. Cell death-inducing DNA fragmentation factor-α-like effector C (CIDEC) is a lipid droplet-associated protein that promotes intracellular triacylglycerol accumulation. In the present study, using gene overexpression and knockd ...
... The induction of plant somatic embryogenesis is often a limiting step for plant multiplication and genetic manipulation in numerous crops. It depends on multiple signaling developmental processes involving phytohormones and the induction of specific genes. The WUSCHEL gene (WUS) is required for the production of plant embryogenic stem cells. To explore a different approach to induce somatic embryo ...
... The expression level of the Cftr and Ocln genes was shown to decrease against an increase in the Nfkb1 gene expression level during the healing of both planar full-thickness excisional and purulonecrotic skin wounds in rats. The restoration of the Ocln gene expression could be mediated by an increase in the Cftr gene expression level, due to a decrease in the mRNA level of the Nfkb1 gene. With the ...
... Selectable marker genes are very important for the screening of target mutants. At least two selectable marker genes are usually required to identify the function of a gene by gene knockout, gene complementation, and gene overexpression. However, there are few selectable marker genes that can be applied to Cordyceps militaris. In order to obtain the selectable marker gene which can be used to C. m ...
... Cofactor availability is often a rate-limiting factor in the bioconversion of xylose to xylitol. The overexpression of pentose phosphate pathway genes and the deletion of Embden–Meyerhof–Parnas pathway genes can modulate the glucose metabolic flux and increase the intracellular NADPH supply, enabling Escherichia coli cells to produce xylitol from corncob hydrolysates. The effects of zwf and/or gnd ...
... Overexpression of normal Ras and its aberrant CpG island methylation in the promoter regions have been shown to direct cells for uncontrolled abnormal growth and bladder tumor formation and therefore, fetched recent attention as a marker of diagnosis and prognosis to predict the biological behavior of urothelial carcinoma of bladder (UCB). Methylation pattern at CpG islands of the promoter regions ...
... Increasing evidence indicates that insect resistance to synthesized insecticides is regulated by the nuclear receptors. However, the underlying mechanisms of this regulation are not clear. Here, we demonstrate that inhibition of hepatocyte nuclear factor 4 (HNF4) confers imidacloprid resistance in the brown planthopper (BPH) Nilaparvata lugens by regulating cytochrome P450 and UDP-glycosyltransfer ...
... Late blight (caused by Phytophthora infestans) poses a serious threat to tomato production but the number of late blight resistance genes isolated from tomato is limited, making resistance gene mining a high research priority. In this study, highly resistant CLN2037E and susceptible No. 5 tomato inbred lines were used to identify late blight resistance genes. Using transcriptome sequencing, we dis ...
geneoverexpression, etc ; cell biology; free radicals; genes; melanin; superoxide anion; Show all 6 Subjects
Abstract:
... Decrease in Tjp 1 gene expression against the background of the activation of free radical processes (increase in the content of superoxide anion-radical) was observed during full-thickness skin wounds' healing in rats. The restoration of the expression level of this gene may be mediated by an increase in the expression level of the Tlr2 gene. The level of Tjp1 gene expression, as well as the cont ...
geneoverexpression, etc ; Bombyx mori; Bombyx mori nucleopolyhedrovirus; cell division; enzyme activation; gene expression regulation; genes; heat-shock protein 90; insect proteins; silkworms; virus replication; Show all 11 Subjects
Abstract:
... Cell division cycle protein 37 (Cdc37) is a molecular chaperone that actively participates in many intracellular physiological and biochemical processes as well as pathogen infection. However, the function of Cdc37 in silkworm cells under Bombyx mori nucleopolyhedrovirus (BmNPV) infection is unknown. We cloned and identified BmCdc37, a Cdc37 gene from B. mori, which is highly conserved among other ...
... Metal elements are widely used in various industrial activities and are considered as common water source contaminants. Thus, the development of cost-effective, simple design and efficient processes for trace metal elements removal from contaminated water sources is of great interest. The effects of cadmium, lead and chromium on growth, biomolecules accumulation and metabolic responses of Amphora ...
geneoverexpression, etc ; Arabidopsis; apples; flowering; genes; gibberellic acid; phosphates; plant growth; starvation; Show all 9 Subjects
Abstract:
... Inorganic phosphate (Pi) starvation severely affects the normal growth and development of plants. Here, a Pi-responsive gene, named MdMYB2 (MDP0000823458), was cloned and functionally identified in apple. Overexpression of MdMYB2 regulated the expression of Pi starvation-induced (PSI) genes and then promoted phosphate assimilation and utilization. The ectopic expression of MdMYB2 in Arabidopsis in ...