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1. A multidomain α-glucan synthetase 2 (AmAgs2) is the key enzyme for pullulan biosynthesis in Aureobasidium melanogenum P16

Author:
Chen, Tie-Jun; Liu, Guang-Lei; Wei, Xin; Wang, Kai; Hu, Zhong; Chi, Zhe; Chi, Zhen-Ming
Source:
International journal of biological macromolecules 2020 v.150 pp. 1037-1045
ISSN:
0141-8130
Subject:
gene overexpression,   etc   ; Aureobasidium; amylases; biosynthesis; exopolysaccharides; genes; glycogen (starch) synthase; hydrolysis; maltotriose; plasma membrane; pullulan;     Show all 11 Subjects
Abstract:
... Pullulan, a biological macromolecule, has many applications. However, it is completely unknown how and where it is synthesized. In this study, it was found that the multidomain AmAgs2 (α-glucan synthase 2) encoded by an AmAGS2 gene in Aureobasidium melanogenum P16 contained the amylase domain (Amy_D), the glycogen synthetase domain (Gys_D) and the transmembrane regions in which the exopolysacchari ...
DOI:
10.1016/j.ijbiomac.2019.10.108

2. Function of c-type cytochromes of Shewanella xiamenensis in enhanced anaerobic bioreduction of Cr(VI) by graphene oxide and graphene oxide/polyvinyl alcohol films

Author:
Li, Yixin; Chen, Zheng; Shi, Yanyan; Luo, Qingliu; Wang, Yiming; Wang, Honghui; Peng, Yajuan; Wang, Haitao; He, Ning; Wang, Yuanpeng
Source:
Journal of hazardous materials 2020 v.387 pp. 122018
ISSN:
0304-3894
Subject:
gene overexpression,   etc   ; Shewanella; chromium; cytochromes; gene expression regulation; genes; graphene; graphene oxide; polyvinyl alcohol;     Show all 9 Subjects
Abstract:
... Graphene-based materials have been demonstrated to facilitate electron extracellular transfer (EET) of Shewanella. In this study, compared to group lacking graphene oxide (GO)-based materials, GO films-added group and graphene oxide/polyvinyl alcohol (GO/PVA) film-added group delivered 2.67- and 3.13-fold increases in the Cr(VI) reduction by Shewanella xiamenensis, respectively. The whole reductio ...
DOI:
10.1016/j.jhazmat.2020.122018

3. Angiopoietin-1 accelerates Alzheimer's disease via FOXA2/PEN2/APP pathway in APP/PS1 mice

Author:
Peng, Zhe; Luo, Yan; Xiao, Zhi-Yong
Source:
Life sciences 2020 v.246 pp. 117430
ISSN:
0024-3205
Subject:
gene overexpression,   etc   ; Alzheimer disease; angiogenesis; angiopoietin-1; cognitive disorders; gene expression regulation; genes; humans; secretion;     Show all 9 Subjects
Abstract:
... Angiopoietin-1 (Ang-1), a regulatory angiogenesis protein and it has been found to be involved in the occurrence and progression of Alzheimer's disease. However, it was still to be addressed the distinctly role and the molecular mechanisms of Ang-1 affects Alzheimer's disease. Our data suggest that Ang-1 aggravated the accumulation of Aβ₄₂ and cognitive decline in APP/PS1 mice. The upregulation of ...
DOI:
10.1016/j.lfs.2020.117430

4. Heterologous overexpression of Nothapodytes foetida strictosidine synthase enhances levels of anti-cancer compound camptothecin in Ophiorrhiza rugosa

Author:
Singh, Sudhir; Kamble, Suchita N.; Satdive, Ramesh K.; Fulzele, Devanand P.
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.1 pp. 67-76
ISSN:
0167-6857
Subject:
gene overexpression,   etc   ; Ophiorrhiza; antineoplastic agents; endangered species; genes; indole alkaloids; mature plants; monoterpenoids; tissues; trees; tryptamine;     Show all 11 Subjects
Abstract:
... Nothapodytes foetida, an endangered tree of Indian origin, is a major source of the anti-cancer monoterpenoid indole alkaloid, camptothecin (CPT). Strictosidine synthase (STR) condenses tryptamine and secologanin to form strictosidine, a universal precursor of terpenoid indole alkaloids including CPT. We cloned full-length str cDNA with an open reading frame of 1059 bp from N. foetida (Nfstr) usin ...
DOI:
10.1007/s11240-020-01767-9

5. Improved milbemycin production by engineering two Cytochromes P450 in Streptomyces bingchenggensis

Author:
Wang, Haiyan; Cheng, Xu; Liu, Yuqing; Li, Shanshan; Zhang, Yanyan; Wang, Xiangjing; Xiang, Wensheng
Source:
Applied microbiology and biotechnology 2020 v.104 no.7 pp. 2935-2946
ISSN:
0175-7598
Subject:
gene overexpression,   etc   ; Streptomyces; biosynthesis; byproducts; cytochrome P-450; furans; genes; milbemycins; prices; transcription (genetics);     Show all 10 Subjects
Abstract:
... Milbemycins and their semisynthetic derivatives are recognized as effective and eco-friendly pesticides, whereas the high price limits their widespread applications in agriculture. One of the pivotal questions is the accumulation of milbemycin-like by-products, which not only reduces the yield of the target products milbemycin A3/A4, but also brings difficulty to the purification. With other analo ...
DOI:
10.1007/s00253-020-10410-8

6. Enhancing titers and productivity of rCHO clones with a combination of an optimized fed-batch process and ER-stress adaptation

Author:
Chandrawanshi, Vikas; Kulkarni, Rohan; Prabhu, Anuja; Mehra, Sarika
Source:
Journal of biotechnology 2020 v.311 pp. 49-58
ISSN:
0168-1656
Subject:
gene overexpression,   etc   ; cell engineering; clones; evolutionary adaptation; genes; metabolic flux analysis; monoclonal antibodies; tunicamycin;     Show all 8 Subjects
Abstract:
... To increase the productivity of rCHO cells, many cell engineering approaches have been demonstrated that over-express or knockout a specific gene to achieve increased titers. In this work, we present an alternate approach, based on the concept of evolutionary adaptation, to achieve cells with higher titers. rCHO cells, producing a monoclonal antibody, are adapted to ER-stress, by continuous cultur ...
DOI:
10.1016/j.jbiotec.2020.02.008

7. Improved production of 3-hydroxypropionic acid in engineered Escherichia coli by rebalancing heterologous and endogenous synthetic pathways

Author:
Lee, Tae-Young; Min, Won-Ki; Kim, Hyo Jin; Seo, Jin-Ho
Source:
Bioresource technology 2020 v.299 pp. 122600
ISSN:
0960-8524
Subject:
gene overexpression,   etc   ; Escherichia coli; aldehyde dehydrogenase; batch fermentation; biochemical pathways; glucose; glycerol; operon; transcription (genetics); xylose;     Show all 10 Subjects
Abstract:
... To improve 3-hydroxypropionic acid (3-HP) production by Escherichia coli, glycerol accumulation needs to be reduced. To accomplish this, we constructed a novel E. coli strain that overexpresses the endogenous aldehyde dehydrogenase gene (puuC) under the control of a strong promoter. The fermentation performance of the engineered strain was significantly improved compared to that of the parental co ...
DOI:
10.1016/j.biortech.2019.122600

8. SRSF1-3, a splicing and somatic hypermutation regulator, controls transcription of IgV genes via chromatin regulators SATB2, UBN1 and histone variant H3.3

Author:
Singh, Amit Kumar; Tamrakar, Anubhav; Jaiswal, Ankit; Kanayama, Naoki; Kodgire, Prashant
Source:
Molecular immunology 2020 v.119 pp. 69-82
ISSN:
0161-5890
Subject:
gene overexpression,   etc   ; RNA; chromatin; gene expression regulation; genes; histones; promoter regions; transcription (genetics); transcription factors; transcriptomics;     Show all 10 Subjects
Abstract:
... SRSF1, a member of the SR protein family, is an important splicing factor and regulator of splicing. Multiple splicing isoforms have been reported for this gene. SRSF1–3, a splicing isoform of SRSF1, is necessary for AID-dependent SHM of IgV genes. However, its precise role in SHM remains enigmatic. Transcriptomic analysis of SRSF1–3 reconstituted cells shows upregulation of transcription factor S ...
DOI:
10.1016/j.molimm.2020.01.005

9. The effects of kanamycin concentration on gene transcription levels in Escherichia coli

Author:
Liu, Xiaozhen; Li, Na; Jia, Minjun; Zhang, Shuangli; Niu, Hao; Li, Qiang; Gu, Pengfei
Source:
3 Biotech 2020 v.10 no.3 pp. 93
ISSN:
2190-572X
Subject:
gene overexpression,   etc   ; Escherichia coli; antibiotic resistance; enzymes; fermentation; fluorescence; kanamycin; operon; plasmids; reporter genes; transcription (genetics);     Show all 11 Subjects
Abstract:
... In the area of microbial production of valuable chemicals, plasmids have been widely applied for overexpressing rate-limiting enzymes with high yields. However, the effect of antibiotic concentrations on the transcription of target genes in E. coli is less involved in previous reports. In this study, we constructed E. coli strains expressing the reporter gene and the kanamycin resistant gene in an ...
DOI:
10.1007/s13205-020-2100-2
PubMed:
32099734
PubMed Central:
PMC7002634

10. Hedgehog signaling promotes lipolysis in adipose tissue through directly regulating Bmm/ATGL lipase

Author:
Zhang, Jie; Liu, Yajuan; Jiang, Kai; Jia, Jianhang
Source:
Developmental biology 2020 v.457 no.1 pp. 128-139
ISSN:
0012-1606
Subject:
gene overexpression,   etc   ; Drosophila; binding sites; carboxylic ester hydrolases; fat body; genomics; lipolysis; mutation; proteins; triacylglycerols;     Show all 10 Subjects
Abstract:
... Hedgehog (Hh) signaling has been shown to regulate multiple developmental processes, however, it is unclear how it regulates lipid metabolism. Here, we demonstrate that Hh signaling exhibits potent activity in Drosophila fat body, which is induced by both locally expressed and midgut-derived Hh proteins. Inactivation of Hh signaling increases, whereas activation of Hh signaling decreases lipid acc ...
DOI:
10.1016/j.ydbio.2019.09.009

11. Functional identification of apple MdMYB2 gene in phosphate-starvation response

Author:
Yang, Yu-Ying; Ren, Yi-Ran; Zheng, Peng-Fei; Qu, Feng-Jia; Song, Lai-Qing; You, Chun-Xiang; Wang, Xiao-Fei; Hao, Yu-Jin
Source:
Journal of plant physiology 2020 v.244 pp. 153089
ISSN:
0176-1617
Subject:
gene overexpression,   etc   ; Arabidopsis; apples; flowering; genes; gibberellic acid; phosphates; plant growth; starvation;     Show all 9 Subjects
Abstract:
... Inorganic phosphate (Pi) starvation severely affects the normal growth and development of plants. Here, a Pi-responsive gene, named MdMYB2 (MDP0000823458), was cloned and functionally identified in apple. Overexpression of MdMYB2 regulated the expression of Pi starvation-induced (PSI) genes and then promoted phosphate assimilation and utilization. The ectopic expression of MdMYB2 in Arabidopsis in ...
DOI:
10.1016/j.jplph.2019.153089

12. The ethylene response factor SmERF8 regulates the expression of SmKSL1 and is involved in tanshinone biosynthesis in Saliva miltiorrhiza hairy roots

Author:
Bai, Zhenqing; Wu, Jiawen; Huang, Wenli; Jiao, Jie; Zhang, Chenlu; Hou, Zhuoni; Yan, Kaijing; Zhang, Xuemin; Han, Ruilian; Liang, Zongsuo; Zhang, Xiujuan
Source:
Journal of plant physiology 2020 v.244 pp. 153006
ISSN:
0176-1617
Subject:
gene overexpression,   etc   ; RNA interference; biosynthesis; cDNA libraries; ethylene; genes; genetically modified organisms; roots; saliva; transcription (genetics);     Show all 10 Subjects
Abstract:
... Saliva miltiorrhiza ethylene response factor (SmERF), predicted to be expressed genome-wide, is the potential regulator of tanshinone biosynthesis. However, few studies have investigated its transcriptional regulation pathways in tanshinone biosynthesis. Here, we report an ethylene response factor (SmERF8), which was screened by the SmKSL1 (a key gene in tanshinone biosynthesis) promoter from the ...
DOI:
10.1016/j.jplph.2019.153006

13. Improved 2,3-butanediol yield and productivity from lignocellulose biomass hydrolysate in metabolically engineered Enterobacter aerogenes

Author:
Kim, Duck Gyun; Yoo, Seok Woo; Kim, Minsun; Ko, Ja Kyong; Um, Youngsoon; Oh, Min-Kyu
Source:
Bioresource technology 2020 v.309 pp. 123386
ISSN:
0960-8524
Subject:
gene overexpression,   etc   ; Enterobacter aerogenes; batch fermentation; biomass; glucose; hydrolysates; lignocellulose; metabolic engineering; operon; succinic acid; sugarcane bagasse; xylose;     Show all 12 Subjects
Abstract:
... We previously engineered Enterobacter aerogenesfor glucose and xylose co-utilization and 2,3-butanediol production. Here, strain EMY-22 was further engineered to improve the 2,3-butanediol titer, productivity, and yield by reducing the production of byproducts. To reduce succinate production, the budABC operon and galP gene were overexpressed, which increased 2,3-butanediol production. For further ...
DOI:
10.1016/j.biortech.2020.123386

14. Pluripotency factor NANOG promotes germ cell maintenance in vitro without triggering dedifferentiation of spermatogonial stem cells

Author:
Yang, Mi; Deng, Bingquan; Geng, Lei; Li, Lufan; Wu, Xin
Source:
Theriogenology 2020 v.148 pp. 68-75
ISSN:
0093-691X
Subject:
gene overexpression,   etc   ; allografting; epigenetics; mice; pachytene stage; spermatids; spermatocytes; spermatogenesis; spermatogonia; spermatozoa; stem cells; testes; transcription factors;     Show all 13 Subjects
Abstract:
... Spermatogonial stem cells (SSCs) are defined as the tissue-specific stem cells in the testes that produce sperm and support life-long spermatogenesis. A culture microenvironment could convert mouse SSCs from unipotent to pluripotent states; however, the underlying mechanism is unclear. NANOG has been considered the decisive transcriptional factor for pluripotency transition of stem cells, but NANO ...
DOI:
10.1016/j.theriogenology.2020.02.028

15. Significance of 195 bp-enhancer of PdCYP51B in the acquisition of Penicillium digitatum DMI resistance and increase of fungal virulence

Author:
de Ramón-Carbonell, Marta; Sánchez-Torres, Paloma
Source:
Pesticide biochemistry and physiology 2020 v.165 pp. 104522
ISSN:
0048-3575
Subject:
gene overexpression,   etc   ; Citrus; Penicillium digitatum; enzymes; fungi; fungicide resistance; gene expression regulation; genes; imazalil; mutants; sterols; transcription (genetics); virulence;     Show all 13 Subjects
Abstract:
... Two sterol 14α-demethylase genes from Penicillium digitatum, PdCYP51A and PdCYP51B, were evaluated and revealed that 95% of Imazalil (IMZ)-resistant isolates carried a 195-bp insertion in the PdCYP51B promoter. We functionally characterized both sterol 14α-demethylases by overexpression. Molecular analysis of overexpression mutants showed that the introduction of PdCYP51B insertion is more stable ...
DOI:
10.1016/j.pestbp.2020.01.003

16. APETALA 2 transcription factor CBX1 is a regulator of mycorrhizal symbiosis and growth of Lotus japonicus

Author:
Liu, Fang; Xu, Yunjian; Wang, Hequn; Zhou, Yuan; Cheng, Beijiu; Li, Xiaoyu
Source:
Plant cell reports 2020 v.39 no.4 pp. 445-455
ISSN:
0721-7714
Subject:
gene overexpression,   etc   ; Lotus corniculatus var. japonicus; biosynthesis; genes; lignin; mutants; mycorrhizae; mycorrhizal fungi; root systems; transcription factors;     Show all 10 Subjects
Abstract:
... KEY MESSAGE: An AP2 family gene CBX1 is involved in mycorrhizal symbiosis and growth of Lotus japonicus. APETALA 2 (AP2) transcriptional regulator is highly conserved in plants. CBX1 from Lotus japonicus is a member of AP2 family. AMF (Arbuscular mycorrhizal fungi) inoculation experiment demonstrated that expression of CBX1 was significantly induced by AMF. Further promoter analysis showed that th ...
DOI:
10.1007/s00299-019-02501-2

17. β-Lactam resistance development affects binding of penicillin-binding proteins (PBPs) of Clostridium perfringens to the fluorescent penicillin, BOCILLIN FL

Author:
Park, Miseon; Sutherland, John B.; Rafii, Fatemeh
Source:
Anaerobe 2020 v.62 pp. 102179
ISSN:
1075-9964
Subject:
gene overexpression,   etc   ; Clostridium perfringens; drug development; drug resistance; enzymes; fluorescence; genes; molecular weight; mutants; mutation; penicillins; proteins; transcription (genetics);     Show all 13 Subjects
Abstract:
... Alteration in the binding of bacterial penicillin-binding proteins (PBPs) to β-lactams is important in the development of drug resistance. The PBPs of wild type Clostridium perfringens ATCC 13124 and three β-lactam-resistant mutants were compared for the ability to bind to a fluorescent penicillin, BOCILLIN FL. The binding of the high molecular weight protein PBP1, a transpeptidase, to BOCILLIN FL ...
DOI:
10.1016/j.anaerobe.2020.102179

18. Overexpression of global regulator LaeA increases secondary metabolite production in Monascus purpureus

Author:
Zhang, Chan; Zhang, Han; Zhu, Qianqian; Hao, Shuai; Chai, Shiyuan; Li, Yinghui; Jiao, Zi; Shi, Jiachen; Sun, Baoguo; Wang, Chengtao
Source:
Applied microbiology and biotechnology 2020 v.104 no.7 pp. 3049-3060
ISSN:
0175-7598
Subject:
gene overexpression,   etc   ; Monascus purpureus; fungi; genes; high performance liquid chromatography; lovastatin; mycelium; pigments; scanning electron microscopy; secondary metabolites; spectrophotometers;     Show all 11 Subjects
Abstract:
... Monascus is a filamentous fungus that produces several secondary metabolites. Here, we investigated the effects of the global regulator LaeA on the synthesis of pigments and monacolin K in Monascus purpureus with spectrophotometer and HPLC methods. The LaeA gene was isolated from M. purpureus M1 to create an overexpression construct. An LaeA-overexpressing strain L3 was with 48.6% higher monacolin ...
DOI:
10.1007/s00253-020-10379-4

19. Overproduction of hydroxytyrosol in Saccharomyces cerevisiae by heterologous overexpression of the Escherichia coli 4-hydroxyphenylacetate 3-monooxygenase

Author:
Muñiz-Calvo, Sara; Bisquert, Ricardo; Puig, Sergi; Guillamón, José Manuel
Source:
Food chemistry 2020 v.308 pp. 125646
ISSN:
0308-8146
Subject:
gene overexpression,   etc   ; Escherichia coli; Saccharomyces cerevisiae; antioxidant activity; carbon; enzymes; genes; metabolism; polyphenols; tyrosine; wine yeasts; wines;     Show all 12 Subjects
Abstract:
... Hydroxytyrosol (HT), which is a polyphenol with a high antioxidant power and many associated health benefits, has been found in wines. Wine yeasts are capable of producing high amounts of the higher alcohol tyrosol, which is the precursor for HT synthesis. We have improved the ability of Saccharomyces cerevisiae to produce HT by heterologously expressing the HpaBC enzyme complex of Escherichia col ...
DOI:
10.1016/j.foodchem.2019.125646

20. Detoxification of ochratoxin A by Lysobacter sp. CW239 and characteristics of a novel degrading gene carboxypeptidase cp4

Author:
Wei, Wei; Qian, Yingying; Wu, Yanbo; Chen, Ying; Peng, Cheng; Luo, Mingzhong; Xu, Junfeng; Zhou, Yu
Source:
Environmental pollution 2020 v.258 pp. 113677
ISSN:
0269-7491
Subject:
gene overexpression,   etc   ; Escherichia coli; Lysobacter; carboxypeptidases; food safety; genetic vectors; mass spectrometry; metabolic detoxification; ochratoxin A; recombinant proteins; toxicity;     Show all 11 Subjects
Abstract:
... Ochratoxin A (OTA) is a potent mycotoxin that frequently contaminates agro-products and threatens food safety. A highly efficient OTA degrading strain Lysobacter sp. CW239 was isolated, and the OTA degradation characteristics were investigated. A novel OTA degrading gene carboxypeptidase cp4 was successfully cloned and characterized from CW239. The heterologous recombinant was constructed by gene ...
DOI:
10.1016/j.envpol.2019.113677