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1. Phylogeny and classification of Leptophlebiidae (Ephemeroptera) with an emphasis on Neotropical fauna

Author:
Monjardim, Marina; Paresque, Roberta; Salles, Frederico F.
Source:
Systematic entomology 2020 v.45 no.2 pp. 415-429
ISSN:
0307-6970
Subject:
genetic markers,   etc   ; Leptophlebiidae; Neotropics; fauna; insect taxonomy; phylogeny;     Show all 6 Subjects
Abstract:
... Mayflies from the family Leptophlebiidae are cosmopolitan and highly diverse morphologically; they are also the largest family in numbers of genera and the second in number of species in the order Ephemeroptera. In spite of their broad diversity and the efforts employed to understand the evolution of this group, the internal classification of Leptophlebiidae remains controversial at all levels. Mo ...
DOI:
10.1111/syen.12402

2. Correction to: Biodegradation of naphthenic acids: identification of Rhodococcus opacus R7 genes as molecular markers for environmental monitoring and their application in slurry microcosms

Author:
Zampolli, J.; Di Canito, A.; Cappelletti, M.; Collina, E.; Lasagni, M.; Di Gennaro, P.
Source:
Applied microbiology and biotechnology 2020 v.104 no.6 pp. 2747
ISSN:
0175-7598
Subject:
genetic markers,   etc   ; Rhodococcus opacus; biodegradation; environmental monitoring; genes; naphthenates; slurries;     Show all 7 Subjects
Abstract:
... The published online version contains mistake in the author list for the author “M. Cappelletti” was incorrectly presented. ...
DOI:
10.1007/s00253-020-10442-0

3. Generating amplicon reads for microbial community assessment with next‐generation sequencing

Author:
Gołębiewski, M.; Tretyn, A.
Source:
Journal of applied microbiology 2020 v.128 no.2 pp. 330-354
ISSN:
1364-5072
Subject:
genetic markers,   etc   ; high-throughput nucleotide sequencing; microbial communities;     Show all 3 Subjects
Abstract:
... Marker gene amplicon sequencing is often preferred over whole genome sequencing for microbial community characterization, due to its lower cost while still enabling assessment of uncultivable organisms. This technique involves many experimental steps, each of which can be a source of errors and bias. We present an up‐to‐date overview of the whole experimental pipeline, from sampling to sequencing ...
DOI:
10.1111/jam.14380

4. Intragenomic variation of rDNA internal transcribed spacers in the endemic Baikal sponge Lubomirskia baikalensis (Pallas, 1776) (Spongillida, Lubomirskiidae): Implications for Porifera barcoding

Author:
Itskovich, V.
Source:
Journal of Great Lakes research 2020 v.46 no.1 pp. 62-66
ISSN:
0380-1330
Subject:
genetic markers,   etc   ; Porifera; concerted evolution; haplotypes; ribosomal DNA; species identification;     Show all 6 Subjects
Abstract:
... Baikal endemic sponges are a species group for which species identification is difficult and the taxonomy is insufficiently developed. The rDNA ITS (internal transcribed spacers) are practical molecular markers for sponges. However, intragenomic polymorphism of the ITS spacers has recently been detected in many organisms including marine and freshwater sponges. We for the first time demonstrate in ...
DOI:
10.1016/j.jglr.2019.10.009

5. Analysis of mtDNA variability in closely related Baikal sponge species for new barcoding marker development

Author:
Yakhnenko, A. S.; Itskovich, V. B.
Source:
Limnology 2020 v.21 no.1 pp. 49-57
ISSN:
1439-8621
Subject:
genetic markers,   etc   ; cryptic speciation; haplotypes; interspecific variation; mitochondrial DNA;     Show all 5 Subjects
Abstract:
... Morphology-based identification and classification of sponge species is complicated, because their morphological features are highly variable. Therefore, molecular markers are ideal tools for delimiting sponge species. In this study, we compared the variability of two sections of COI (the standard 5′-end fragment and the I3-M11 fragment) for closely related Baikal sponge species. Although the mean ...
DOI:
10.1007/s10201-019-00599-7

6. Host-specific mitochondrial DNA markers for tracking the sources of fecal pollution

Author:
Malla, Bikash; Haramoto, Eiji
Source:
Current opinion in environmental science & health 2020 v.16 pp. 34-46
ISSN:
2468-5844
Subject:
genetic markers,   etc   ; epithelial cells; feces; host specificity; hosts; mitochondrial DNA; pollution;     Show all 7 Subjects
Abstract:
... Epithelial cells that contain a large number of mitochondrial DNA (mtDNA) are shed in host feces. Recently, researchers have developed mtDNA markers targeting mtDNA of specific hosts and applied them to microbial source tracking (MST) for different environmental waters. This review describes the performance, persistence in environmental waters, and correlations of host-specific mtDNA markers with ...
DOI:
10.1016/j.coesh.2020.02.006

7. Zebrafish can regenerate endoskeleton in larval pectoral fin but the regenerative ability declines

Author:
Yoshida, Keigo; Kawakami, Koichi; Abe, Gembu; Tamura, Koji
Source:
Developmental biology 2020 v.463 no.2 pp. 110-123
ISSN:
0012-1606
Subject:
genetic markers,   etc   ; Danio rerio; adults; amputation; chondrocytes; ectoderm; epithelium; genes; larvae; larval development; mammals; models;     Show all 12 Subjects
Abstract:
... We show for the first time endoskeletal regeneration in the developing pectoral fin of zebrafish. The developing pectoral fin contains an aggregation plate of differentiated chondrocytes (endochondral disc; primordium for endoskeletal components, proximal radials). The endochondral disc can be regenerated after amputation in the middle of the disc. The regenerated disc sufficiently forms endoskele ...
DOI:
10.1016/j.ydbio.2020.04.010

8. Changes in the phospholipid molecular species in water-boiled salted duck during processing based on shotgun lipidomics

Author:
Li, Cong; Li, Xinfu; Huang, Qianli; Zhuo, You; Xu, Baocai; Wang, Zhouping
Source:
Food research international 2020 v.132 pp. 109064
ISSN:
0963-9969
Subject:
genetic markers,   etc   ; Pekin; boiling; duck meat; ducks; phosphatidylcholines; phosphatidylethanolamines; phosphatidylglycerols; phosphatidylinositols; phosphatidylserines; raw meat;     Show all 11 Subjects
Abstract:
... Knowledge of the changes in the phospholipid molecular species during processing is helpful to understanding the complicated mechanisms of lipid degradation and transformation. The shotgun lipidomics strategy was utilized to analyze the phospholipid (PL) molecular species in raw Pekin duck and the subsequent dynamic changes that occur during the processing of water-boiled salted duck (WSD). Only 1 ...
DOI:
10.1016/j.foodres.2020.109064

9. Development and Maintenance of Tropical Gynoecious Inbred Lines in Cucumber (Cucumis sativus) and Validation by DNA Markers

Author:
Bommesh, J. C.; Pitchaimuthu, M.; Ravishankar, K. V.; Varalakshmi, B.
Source:
Agricultural research 2020 v.9 no.2 pp. 161-168
ISSN:
2249-720X
Subject:
genetic markers,   etc   ; Cucumis sativus; cucumbers; horticulture; hybrids; inbred lines; microsatellite repeats; pedigree; phenotype; pickling; slicing; India;     Show all 12 Subjects
Abstract:
... Gynoecious parthenocarpic hybrids in cucumber (Cucumis sativus L.) have great importance for its successful cultivation under protected conditions to enhance productivity and quality. Work was undertaken to develop and maintain the gynoecious inbred lines from the gynoecious parthenocarpic cucumber hybrids (Silyon hybrid, Pickling cucumber-1 (PC-1), Pickling cucumber-2 (PC-2) and Pune cucumber hyb ...
DOI:
10.1007/s40003-019-00423-9

10. Morphological description of Opalina obtrigonoidea Metcalf, 1923 (Heterokonta, Opalinea) from Duttaphrynus melanostictus and evaluation of the ITS region as a suitable genetic marker for inter-species identification in Opalina

Author:
Zhao, Weishan; Hu, Guangran; Ponce-Gordo, Francisco; Zou, Hong; Li, Wenxiang; Wu, Shangong; Li, Ming; Wang, Guitang
Source:
Parasitology international 2020 v.76 pp. 102103
ISSN:
1383-5769
Subject:
genetic markers,   etc   ; Opalinea; genes; internal transcribed spacers; rectum; redescriptions; ribosomal DNA; sequence analysis; toads;     Show all 9 Subjects
Abstract:
... The redescription of Opalina obtrigonoidea Metcalf, 1923, collected from the rectum of the toads Duttaphrynus melanostictus, is presented in this paper based on detailed morphological information and molecular data. Our results revealed that O. obtrigonoidea varies greatly in body dimensions. Its morphological characteristics allow its differentiation from Opalina undulata. Surprisingly, we sequen ...
DOI:
10.1016/j.parint.2020.102103

11. A general strategy for highly sensitive analysis of genetic biomarkers at single-base resolution with ligase-based isothermally exponential amplification

Author:
Wang, Hui; Wang, Honghong; Sun, Yuanyuan; Liu, Xiaoling; Liu, Ye; Wang, Chengli; Zhang, Pengbo; Li, Zhengping
Source:
Talanta 2020 v.212 pp. 120754
ISSN:
0039-9140
Subject:
genetic markers,   etc   ; DNA; DNA probes; RNA; diagnostic techniques; ligases; precision medicine;     Show all 7 Subjects
Abstract:
... Robust, reliable, and sensitively quantitative detection of genetic biomarkers at single-base resolution has the potential to revolutionize medical diagnostics, especially for precision medicine. Here, taking the advantages of the high specificity of ligase reaction and the powerful amplification features of the isothermally exponential amplification, we have demonstrated a novel methodology to se ...
DOI:
10.1016/j.talanta.2020.120754

12. Novel Variants in the HMGA2 Gene Are Associated With Withers Height in Debao Pony

Author:
Liu, Shuqin; Jiang, Shunyan; Dong, Xiang Gui; Cui, Ran; Ling, Yao; Zhao, Chunjiang
Source:
Journal of equine veterinary science 2020 v.88 pp. 102948
ISSN:
0737-0806
Subject:
genetic markers,   etc   ; DNA; exons; genetic variation; horse breeding; horses; introns; mutation; withers; China;     Show all 10 Subjects
Abstract:
... The Debao pony is a well-known dwarf horse breed in China. High-mobility group AT-hook 2 (HMGA2) gene is regarded as one of the important candidate genes regulating body height in horses. The aim of this study was to study the association between mutations in HMGA2 gene and withers height in Debao ponies. The polymorphisms in all exons and partial introns of the HMGA2 gene were screened with seque ...
DOI:
10.1016/j.jevs.2020.102948

13. Combined molecular and morphological data provide insights into the evolution and classification of Chilocorini ladybirds (Coleoptera: Coccinellidae)

Author:
Li, Wenjing; Łączyński, Piotr; Escalona, Hermes E.; Eberle, Jonas; Huo, Lizhi; Chen, Xiaosheng; Huang, Weidong; Chen, Bingxu; Ahrens, Dirk; Ślipiński, Adam; Tomaszewska, Wioletta; Wang, Xingmin
Source:
Systematic entomology 2020 v.45 no.2 pp. 447-463
ISSN:
0307-6970
Subject:
genetic markers,   etc   ; Bayesian theory; Chilocorus; Cretaceous period; Paleogene period; adults; biological control agents; monophyly; statistical analysis;     Show all 9 Subjects
Abstract:
... Ladybirds of the cosmopolitan tribe Chilocorini prey mainly on coccids and include several important biocontrol agents. The phylogenetic relationships of Chilocorini are poorly known. In this paper, we provide a phylogenetic reconstruction of Chilocorini containing all 27 genera based on five molecular markers and 86 adult morphological characters. Morphological character states were mapped on the ...
DOI:
10.1111/syen.12409

14. Phylogeny of the genus Pinnixa White, 1846 (Crustacea, Brachyura, Pinnotheridae) and allies inferred from mitochondrial and nuclear molecular markers, with generic reassignment of twenty-one species

Author:
Theil, Emma Palacios; Felder, Darryl L.
Source:
Zoosystema 2020 v.42 no.6 pp. 85-103
ISSN:
1280-9551
Subject:
genetic markers,   etc   ; Pinnotheridae; genes; histones; mitochondria; new genus; polyphyly; statistical analysis;     Show all 8 Subjects
Abstract:
... We used mitochondrial 16S-NADH1 complex, mitochondrial 12S, and nuclear histone 3 genes to infer a molecular-based phylogeny, which allowed us to study phylogenetic relationships between species of PinnixaWhite, 1846sensu lato and other closely related pinnotherids. Polyphyly of Pinnixa s.l. was confirmed by maximum likelihood analyses. By our restricted definition, the genus Pinnixa sensu stricto ...
DOI:
10.5252/zoosystema2020v42a6

15. Multiallelic marker system for traceability of Black Slavonian pig meat

Author:
Kušec, Ivona Djurkin; Gvozdanović, Kristina; Djurkin Kušec, Ivona; Margeta, Polonca; Salajpal, Krešimir; Džijan, Snježana; Bošnjak, Zinka; Kušec, Goran
Source:
Food control 2020 v.109 pp. 106917
ISSN:
0956-7135
Subject:
genetic markers,   etc   ; Black Slavonian; fraud; genotype; loci; meat; probability; swine; traceability;     Show all 9 Subjects
Abstract:
... Meat from Black Slavonian pig (Crna slavonska, CS), an autochthonous Croatian breed, is used for the production of high-quality products, which makes it an interesting target for meat fraud. The aim of the study was to develop a set of microsatellite markers suitable for precise genetic traceability of the meat originating from this breed. Initially, 23 microsatellite markers were selected and gro ...
DOI:
10.1016/j.foodcont.2019.106917

16. Species delimitation within Campomanesia (Myrtaceae) using morphology and amplification profiles of microsatellite markers

Author:
Luber, Jaquelini; Christ, Jheniffer Abeldt; Ferreira, Marcia Flores da Silva; Carrijo, Tatiana Tavares
Source:
Revista brasileira de botânica 2020 v.43 no.1 pp. 131-137
ISSN:
0100-8404
Subject:
genetic markers,   etc   ; Campomanesia; DNA fingerprinting; cluster analysis; heat; microsatellite repeats; plant identification; regression analysis; species identification;     Show all 9 Subjects
Abstract:
... Campomanesia is a well-circumscribed genus within Myrtaceae, but the boundaries among some of its species remain poorly understood. Microsatellite amplification profiles have proven useful DNA fingerprinting for plant species identification, but have never been applied to the taxonomy of this genus. Here we studied 12 species of Campomanesia, including a set of related and non-related species, as ...
DOI:
10.1007/s40415-020-00583-x

17. Development of 22 Microsatellite Markers for Assessing Hybridization in the Genus Gekko (Squamata: Gekkonidae)

Author:
Okamoto, Kota; Kurita, Takaki; Nagano, Masahiro; Sato, Yukuto; Aoyama, Hiroaki; Saitoh, Seikoh; Shinzato, Naoya; Toda, Mamoru
Source:
Current herpetology 2020 v.39 no.1 pp. 66-74
ISSN:
1881-1019
Subject:
genetic markers,   etc   ; Gekko; alleles; introgression; loci; planning; polymerase chain reaction; Japan;     Show all 8 Subjects
Abstract:
... Gekko yakuensis and G. tawaensis, both endemic to western Japan, are threatened by genetic introgression from G. hokouensis and G. japonicus, respectively. To know detailed situation of their hybridizations for planning relevant conservation measures, development of sensitive genetic markers is desired. We here developed microsatellite markers based on the sequences obtained from G. hokouensis usi ...
DOI:
10.5358/hsj.39.66

18. Evolution of an Eleusine-Specific Subgroup of Pyricularia oryzae Through a Gain of an Avirulence Gene

Author:
Asuke, Soichiro; Tanaka, Masaki; Hyon, Gang-Su; Inoue, Yoshihiro; Vy, Trinh Thi Phuong; Niwamoto, Daisuke; Nakayashiki, Hitoshi; Tosa, Yukio
Source:
Molecular plant-microbe interactions 2020 v.33 no.2 pp. 153-165
ISSN:
0894-0282
Subject:
genetic markers,   etc   ; DNA fragmentation; Eleusine coracana; Eragrostis; Magnaporthe oryzae; genes; pathotypes; phylogeny; virulence;     Show all 9 Subjects
Abstract:
... Eleusine isolates (members of the Eleusine pathotype) of Pyricularia oryzae are divided into two subgroups, EC-I and EC-II, differentiated by molecular markers. A multilocus phylogenetic analysis revealed that these subgroups are very close to Eragrostis isolates. EC-II and Eragrostis isolates were exclusively virulent on finger millet and weeping lovegrass, respectively, while EC-I isolates were ...
DOI:
10.1094/MPMI-03-19-0083-R

19. Screening of Polymorphic SSR Molecular Markers Between Resistant and Susceptible Parents for Localization of Brown Rust Resistance Gene

Author:
Shan, Hongli; Li, Wenfeng; Huang, Yingkun; Wang, Xiaoyan; Zhang, Rongyue; Li, Jie; Luo, Zhiming; Yin, Jiong; Cang, Xiaoyan
Source:
Sugar tech 2020 v.22 no.1 pp. 1-7
ISSN:
0972-1525
Subject:
genetic markers,   etc   ; Puccinia melanocephala; breeding; chromosome mapping; leaf rust; microsatellite repeats; parents; resistance genes; screening; sugarcane;     Show all 10 Subjects
Abstract:
... Sugarcane brown rust induced by Puccinia melanocephala is an important global disease. Exploring novel resistance genes and breeding varieties with durable resistance is the most economical and effective way of controlling brown rust. The establishment of complete and high-density molecular genetics maps is a key prerequisite for the discovery and location of sugarcane brown rust resistance genes. ...
DOI:
10.1007/s12355-019-00750-9

20. Phylogenetic position of Pleurogenoides species (Plagiorchiida: Pleurogenidae) from the duodenum of Indian skipper frog, Euphlyctis cyanophlyctis (Amphibia: Dicroglossidae) inhabiting the Western Ghats, India

Author:
Chaudhary, A.; Shinad, K.; Prasadan, P. K.; Singh, H. S.
Source:
Helminthologia 2020 v.57 no.1 pp. 71-77
ISSN:
1336-9083
Subject:
genetic markers,   etc   ; Euphlyctis; Plagiorchiida; cations; duodenum; frogs; genes; hosts; phylogeny; polymerase chain reaction; ribosomal RNA; India;     Show all 12 Subjects
Abstract:
... Two species of digenetic trematodes of the genus Pleurogenoides viz., P. cyanophlycti Shinad & Prasadan (2018a) and P. euphlycti Shinad & Prasadan (2018b) have been described from India. Information regarding the molecular data of various species of the genus Pleurogenoides Travassos, 1921 is virtually lacking. This study addresses the application of molecular markers to validate the phylogenetic ...
DOI:
10.2478/helm-2020-0006
PubMed:
32063743
PubMed Central:
PMC6996260