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You searched for: Subject "genetic vectors" Remove constraint Subject: "genetic vectors"
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1. Bioprocess characterization of virus‐like particle production with the insect cell baculovirus expression system at nanoparticle level

Author:
Eduard Puente‐Massaguer; Irene González‐Domínguez; Florian Strobl; Reingard Grabherr; Gerald Striedner; Martí Lecina; Francesc Gòdia
Source:
Journal of chemical technology and biotechnology 2022 v.97 no.9 pp. 2456-2465
ISSN:
0268-2575
Subject:
genetic vectors,   etc   ; DNA; bioprocessing; bioreactors; cell lines; drugs; gene therapy; insects;     Show all 8 Subjects
Abstract:
... Background. Virus‐like particles (VLPs) are a multivalent platform showing great promise for the development of vaccines, gene therapy, diagnostic, and drug delivery approaches. Particularly, HIV‐1 Gag VLPs provide a robust and flexible scaffold for the presentation of a variety of antigens. The insect cell baculovirus expression vector system (BEVS) is nowadays one of the reference systems to pro ...
DOI:
10.1002/jctb.7105

2. Increase recombinant antibody yields through optimizing vector design and production process in CHO cells

Author:
Yongxiao Yang; Zhengmei Li; Qin Li; Kai Ma; Yan Lin; Huigen Feng; Tianyun Wang
Source:
Applied microbiology and biotechnology 2022 v.106 no.13-16 pp. 4963-4975
ISSN:
0175-7598
Subject:
genetic vectors,   etc   ; Cricetulus griseus; antibody formation; biotechnology; microbiology; necrosis; neoplasms; recombinant antibodies;     Show all 8 Subjects
Abstract:
... Chinese hamster ovary (CHO) cells are the most commonly used host cells for the production of recombinant monoclonal antibodies (mAbs) due to their several advantages. Although the yields of recombinant mAbs can be greatly increased by some strategies, such as medium formulation, culture conditions, and cell engineering, most studies focused on either upstream design or downstream processes. In th ...
DOI:
10.1007/s00253-022-12051-5

3. Cloning and functional characterization of arsenite oxidase (aoxB) gene associated with arsenic transformation in Pseudomonas sp. strain AK9

Author:
Ghanshyam Kumar Satyapal; Raghawendra Kumar; Sanjeev Kumar; Ravi Shankar Singh; Prashant; Rajesh Kumar Ranjan; Kundan Kumar; Amrit Kumar Jha; Nater Pal Singh; Rizwanul Haque; Asheesh Shanker; Nitish Kumar
Source:
Gene 2022 pp. 146926
ISSN:
0378-1119
Subject:
genetic vectors,   etc   ; DNA; Pseudomonas; Thiomonas arsenivorans; arsenates; arsenic; arsenites; genes; oxidoreductases; phylogeny; toxicity; India;     Show all 12 Subjects
Abstract:
... Arsenic transforming bacterial strains belong to genus Pseudomonas sp.AK9 (KY569424), were isolated from the middle Gangetic plains of Bihar, India. The Pseudomonas sp. AK9 strains were able to transform toxic arsenite to a less toxic arsenate. In the present work, the presence of different arsenic resistance genes (aoxB, arsB, acr3 and aoxAB) were observed in isolated strain. Furthermore, the aox ...
DOI:
10.1016/j.gene.2022.146926

4. Multifunctional Therapeutic Cyclodextrin-Appended Dendrimer Complex for Treatment of Systemic and Localized Amyloidosis

Author:
Masamichi Inoue; Taishi Higashi; Yuya Hayashi; Risako Onodera; Kazuya Fujisawa; Toru Taharabaru; Ryoma Yokoyama; Kenta Ouchi; Yohei Misumi; Mitsuharu Ueda; Yasuteru Inoue; Mineyuki Mizuguchi; Takashi Saito; Takaomi C. Saido; Yukio Ando; Hidetoshi Arima; Keiichi Motoyama; Hirofumi Jono
Source:
ACS applied materials & interfaces 2022 v.14 no.36 pp. 40599-40611
ISSN:
1944-8252
Subject:
genetic vectors,   etc   ; amyloid; amyloidosis; dendrimers; drugs; humans; prealbumin; protein synthesis; small interfering RNA; therapeutics;     Show all 10 Subjects
Abstract:
... Amyloidosis pathologically proceeds via production of amyloidogenic proteins by organs, formation of protein aggregates through structural changes, and their deposition on tissues. A growing body of evidence demonstrates that amyloidosis generally develops through three critical pathological steps: (1) production of amyloid precursor proteins, (2) amyloid formation, and (3) amyloid deposition. How ...
DOI:
10.1021/acsami.2c09913

5. Analysis of the functional gene of degrading BDE-47 by Acinetobacter pittii GB-2 based on transcriptome sequencing

Author:
Zi-Wei Li; Ji-Hua Wang
Source:
Gene 2022 v.844 pp. 146826
ISSN:
0378-1119
Subject:
genetic vectors,   etc   ; Acinetobacter; diphenyl ethers; gene expression regulation; genes; hydroxylation; metabolism; phenol; toxicity; transcriptome;     Show all 10 Subjects
Abstract:
... 2,2′,4,4′-tetrabrominated diphenyl ether (BDE-47) is one of the most widely distributed PBDEs. BDE-47 is also the most abundant in organisms and the most toxic to humans and animals. Herein, we have studied the pathway of BDE-47 degradation and gene involvement in Acinetobacter pittii GB-2. This degradation is dominated by hydroxylation, resulting in hydroxylated products 6-OH-BDE-47, 5-OH-BDE-47 ...
DOI:
10.1016/j.gene.2022.146826

6. Cell-penetrating peptide-mediated transfection of proteins into tissues of Arabidopsis and Chinese cabbage

Author:
Han Wu; Zhipeng Zhang; Kai Zhu; Yanqiu Wang; Fulai Ke; Fei Zhang; Ying Zhao; Yun Zhang; Hui Feng
Source:
In vitro cellular & developmental biology 2022 v.58 no.1 pp. 28-34
ISSN:
1054-5476
Subject:
genetic vectors,   etc   ; Arabidopsis; Chinese cabbage; DNA; Escherichia coli; arginine; cysteine; histidine; peptides; ribonucleoproteins; transfection;     Show all 11 Subjects
Abstract:
... Cell-penetrating peptides (CPPs) are a class of short peptides, which have the ability of intracellular delivery. CPPs can also be used as an ideal transfection tool whereby biomacromolecules can be transferred as cargo into recipient cells. Here, a synthesized cell-penetrating peptide (R9)-mediated DNA-free transfection is presented. DNA sequences of nine tandem arginine (R9), one cysteine (cys), ...
DOI:
10.1007/s11627-021-10221-0

7. Development of a Taxon-Specific Real-Time PCR Method Targeting the Bacillus subtilis Group to Strengthen the Control of Genetically Modified Bacteria in Fermentation Products

Author:
Marie-Alice Fraiture; Andrea Gobbo; Nina Papazova; Nancy H. C. Roosens
Source:
Fermentation 2022 v.8 no.2 pp. -
ISSN:
2311-5637
Subject:
genetic vectors,   etc   ; Bacillus subtilis; DNA; antibiotic resistance; fermentation; markets; quantitative polymerase chain reaction; traceability;     Show all 8 Subjects
Abstract:
... Most of the bacteria that are used to produce fermentation products, such as enzymes, additives and flavorings, belong to the Bacillus subtilis group. Recently, unexpected contaminations with unauthorized genetically modified (GM) bacteria (viable cells and associated DNA) that were carrying antimicrobial resistance (AMR) genes was noticed in several microbial fermentation products that have been ...
DOI:
10.3390/fermentation8020078

8. Homologous recombination risk in baculovirus expression vector system

Author:
Jianan Huang; Huanlei Liu; Xiaodong Xu
Source:
Virus research 2022 v.321 pp. 198924
ISSN:
0168-1702
Subject:
genetic vectors,   etc   ; DNA fragmentation; fluorescence; homologous recombination; research; risk; signal peptide; viral genome; viruses;     Show all 9 Subjects
Abstract:
... The baculovirus expression vector system (BEVS) is widely used for producing recombinant proteins. To achieve high expression level of recombinant proteins, baculoviral elements, such as enhancers, promoters, signal peptide coding sequences and 3′-UTR, have been extensively employed. There is a recombination risk derived from homologous sequences between viral genome and functional baculovirus-der ...
DOI:
10.1016/j.virusres.2022.198924

9. Molecular characterization, expression and anti-tumor function analysis of yak IFITM2 gene

Author:
Haipeng Wang; Li Wang; Xiaolin Luo; Jiuqiang Guan; Xiangfei Zhang; Ling Zhang; Yi Xiang
Source:
International journal of biological macromolecules 2022 v.209 pp. 405-412
ISSN:
0141-8130
Subject:
genetic vectors,   etc   ; B-lymphocytes; genes; lungs; molecular weight; nucleotide sequences; rapamycin; transmembrane proteins; yaks;     Show all 9 Subjects
Abstract:
... IFITM2 is interferon-induced transmembrane protein 2, which plays an extremely key role in anti-tumor and anti-virus diseases. In this study, the 602 bp cDNA sequence of the yak (Bos grunniens) IFITM2 (BgIFITM2) gene was obtained. Moreover, the prokaryotic expression vector of BgIFITM2 protein was constructed and expressed successfully, with a molecular weight of 33.680 kDa. The proliferation acti ...
DOI:
10.1016/j.ijbiomac.2022.03.212

10. Heterologous expression of a cryptic gene cluster from a marine proteobacterium Thalassomonas actiniarum affords new lanthipeptides thalassomonasins A and B

Author:
Chanaphat Thetsana; Shinta Ijichi; Issara Kaweewan; Hiroyuki Nakagawa; Shinya Kodani
Source:
Journal of applied microbiology 2022 v.132 no.5 pp. 3629-3639
ISSN:
1364-5072
Subject:
genetic vectors,   etc   ; Escherichia coli; Thalassomonas; biosynthesis; heterologous gene expression; microbiology; multigene family; nucleotide sequences; plasmids;     Show all 9 Subjects
Abstract:
... AIMS: The aim of this study was to utilize a cryptic biosynthetic gene cluster (BGC) of a marine proteobacterium Thalassomonas actiniarum for production of new lanthipeptides by heterologous expression system. METHODS AND RESULTS: Based on genome mining, a new BGC of class I lanthipeptide was found in the genome sequence of a marine proteobacterium T. actiniarum. Molecular cloning was performed to ...
DOI:
10.1111/jam.15491

11. Actin Contributes to the Hyperexpression of Baculovirus Polyhedrin (polh) and p10 as a Component of Transcription Initiation Complex (TIC)

Author:
Nan Chen; Guanping Chen; Xiangshuo Kong; Xiaofeng Wu
Source:
Viruses 2022 v.14 no.1 pp. -
ISSN:
1999-4915
Subject:
genetic vectors,   etc   ; Baculoviridae; DNA; DNA-directed RNA polymerase; actin; chromatin immunoprecipitation; luciferase; physiological transport; transcription initiation;     Show all 9 Subjects
Abstract:
... Hyperexpression of polh and p10, two very late genes, is one of the remarkable characteristics in the baculovirus life cycle. However, the mechanisms underlying the hyperexpression of these two genes are still incompletely understood. In this study, actin was identified as a highly potential binding partner of polh and p10 promoters by conducting DNA pull-down and LC–MS/MS analyses. Inhibiting act ...
DOI:
10.3390/v14010153

12. Rapid titration of recombinant baculoviruses based on NanoLuc secretion in early infection

Author:
Jige Li; Meng Xu; Juan Sun; Junjun Li; Jianqing Chen; Qin Chen; Jian Chen
Source:
Journal of virological methods 2022 v.307 pp. 114565
ISSN:
0166-0934
Subject:
genetic vectors,   etc   ; Baculoviridae; culture media; gene transfer; genes; luciferase; protein synthesis; recombinant proteins; secretion; titration; viruses;     Show all 11 Subjects
Abstract:
... The baculovirus expression vector system has become a powerful tool for recombinant protein production and gene delivery. However, existing titration methods for baculovirus are not economical in terms of test time and cost. A titration method based on NanoLuc secretion that allows for titration of recombinant baculoviruses at 4 h post infection (hpi) is described. In the assay, the envelop protei ...
DOI:
10.1016/j.jviromet.2022.114565

13. Challenges of expressing recombinant human tissue factor as a secreted protein in Pichia pastoris

Author:
Mohammad Jalili-Nik; Mohammad Soukhtanloo; Majid Mojarrad; Mohammad Hadi Sadeghian; Baratali Mashkani
Source:
Preparative biochemistry & biotechnology 2022 v.52 no.9 pp. 1001-1007
ISSN:
1532-2297
Subject:
genetic vectors,   etc   ; Komagataella pastoris; Western blotting; amino acid sequences; biotechnology; databases; humans; polyacrylamide gel electrophoresis; protein secretion; prothrombin;     Show all 10 Subjects
Abstract:
... Tissue factor (TF) is the core reagent in the prothrombin time (PT) assay. In this study, expression and α-factor mediated secretion of three forms of tissue factor (full-length TF (Full-TF), extracellular plus transmembrane domain (TED-TF), and only extracellular domain (ED-TF) were investigated in Pichia pastoris. The amino acid sequence of TF was obtained from the UniProt database, back-transla ...
DOI:
10.1080/10826068.2021.2023823

14. Heterologous expression of human C‐reactive protein in the green alga Chlamydomonas reinhardtii

Author:
Wei Liang; Junjie Qiu; Mengping Zhang; Chuan Wang
Source:
Journal of food biochemistry 2022 v.46 no.2 pp. e14067
ISSN:
0145-8884
Subject:
genetic vectors,   etc   ; C-reactive protein; Chlamydomonas reinhardtii; genes; glass; heterologous gene expression; humans; markets; protein synthesis; value added;     Show all 10 Subjects
Abstract:
... C‐reactive protein (CRP) participates in human inflammatory responses and is an important indicator in clinical diagnoses. At present, the use of monoclonal antibodies to detect CRP in the human body is high, but they are unstable and expensive. Understanding the CRP expression pathway is of great significance for developing CRP tests and reagents. Chlamydomonas reinhardtii is a model organism tha ...
DOI:
10.1111/jfbc.14067

15. Development of a heterologous gene expression vector in plants based on an infectious clone of a tobravirus, pepper ringspot virus

Author:
Moana Lima Tavares‐Esashika; Ravi Narayan Souza Campos; Matheus Hideki Kihara Maeda; Lucas Hideo Hataka Koyama; Pedro Ricardo Vieira Hamann; Eliane Ferreira Noronha; Tatsuya Nagata
Source:
Annals of applied biology 2022 v.181 no.1 pp. 107-116
ISSN:
0003-4746
Subject:
genetic vectors,   etc   ; Nicotiana benthamiana; Pepper ringspot virus; biotechnology; coat proteins; green fluorescent protein; heterologous gene expression; reporter genes; xylanases;     Show all 9 Subjects
Abstract:
... Heterologous gene expression systems are important tools for biotechnology, especially due to the increasing demands for mass production of pharmaceutical proteins. In this study, we assessed the use of the infectious clone of a tobravirus, pepper ringspot virus (PepRSV), as a heterologous gene expression tool in Nicotiana benthamiana plants. The insert region of gene of interest was evaluated in ...
DOI:
10.1111/aab.12758

16. Metallothionein (MT1): A molecular stress marker in chickpea enhances drought and heavy metal stress adaptive efficacy in transgenic plants

Author:
Sanoj Kumar; Ankita Yadav; Rita Verma; Arvind Kumar Dubey; Shiv Narayan; Ankesh Pandey; Anshu Sahu; Sudhakar Srivastava; Indraneel Sanyal
Source:
Environmental and experimental botany 2022 v.199 pp. 104871
ISSN:
0098-8472
Subject:
genetic vectors,   etc   ; chickpeas; cultivars; drought; genes; heavy metals; metallothionein; nitrogen fixation; photosynthesis; stomatal conductance; stress tolerance; water use efficiency;     Show all 12 Subjects
Abstract:
... Metallothioneins (MTs) are diverse class of cysteine-rich proteins having metal-chelation properties. The role of MTs has been demonstrated in different abiotic stresses and MTs have been designated as biomolecular stress markers. Chickpea is an important legume crop supplying proteins to humans, as well as acting as great soil-binders along with nitrogen-fixation capability. The present research ...
DOI:
10.1016/j.envexpbot.2022.104871

17. Molecular cloning, expression and appetite regulation function of adiponectin in Siberian sturgeon (Acipenser baerii)

Author:
Ni Tang; Ya Li; Yingzi Li; Yanling Liu; Shupeng Zhang; Shaoqi Xu; Mei Wang; Bin Wang; Hu Chen; Xin Zhang; Bo Zhou; Defang Chen; Zhiqiong Li
Source:
International journal of biological macromolecules 2022 v.214 pp. 360-369
ISSN:
0141-8130
Subject:
genetic vectors,   etc   ; Acipenser baerii; Escherichia coli; adiponectin; amino acids; appetite; fasting; fish; food intake; gene expression; genes; hypothalamus; intestines;     Show all 13 Subjects
Abstract:
... Adiponectin (AdipoQ) as an adipocytokine has the potential to regulate feeding behavior, but the information about adipoq in fish is limited. In this study, Siberian sturgeon adiponectin (Ssadipoq) gene was cloned encoding 264 amino acids. The amino acid identity of SsAdipoQ was low compared with that of mammals, birds, amphibians and teleost fishes. The expression of Ssadipoq in the hypothalamus ...
DOI:
10.1016/j.ijbiomac.2022.06.097

18. Improved high-throughput screening technique to rapidly isolate Chlamydomonas transformants expressing recombinant proteins

Author:
Ashley E. Sproles; Anthony Berndt; Francis J. Fields; Stephen P. Mayfield
Source:
Applied microbiology and biotechnology 2022 v.106 no.4 pp. 1677-1689
ISSN:
0175-7598
Subject:
genetic vectors,   etc   ; Chlamydomonas reinhardtii; antibiotics; biotechnology; fluorescence; gene expression; microalgae; microbiology; nuclear genome; phenotype; protein synthesis; recombinant proteins; transgenes;     Show all 13 Subjects
Abstract:
... The single-celled eukaryotic green alga Chlamydomonas reinhardtii has long been a model system for developing genetic tools for algae, and is also considered a potential platform for the production of high-value recombinant proteins. Identifying transformants with high levels of recombinant protein expression has been a challenge in this organism, as random integration of transgenes into the nucle ...
DOI:
10.1007/s00253-022-11790-9

19. Preparation of polyclonal antibody against phosphatidylethanolamine binding protein 1 recombinant protein and its functional verification in pulmonary hypertension syndrome in broilers

Author:
Qingqing Li; Yueming Gu; Xiaona Gao; Xiaoquan Guo; Cheng Huang; Pei Liu; Guoliang Hu; Guyue Li; Weile Fang; Wanrui Mai; Cong Wu; Zheng Xu; Feixiang Huang; Ping Liu
Source:
International journal of biological macromolecules 2022 v.213 pp. 19-26
ISSN:
0141-8130
Subject:
genetic vectors,   etc   ; Western blotting; angiogenesis; ascites; fluorescent antibody technique; hypertension; metastasis; neoplasms; phosphatidylethanolamines; polyclonal antibodies; pulmonary artery; recombinant proteins;     Show all 12 Subjects
Abstract:
... Pulmonary hypertension syndrome (PHS) is a disease that is difficult to overcome for fast-growing broilers. It causes pulmonary vascular remodeling and ascites in broilers. As a classical inhibitor of cancer metastasis, phosphatidylethanolamine binding protein 1 (PEBP1) regulates angiogenesis in the process of tumor metastasis through multiple signal pathways. However, whether PEBP1 can regulate p ...
DOI:
10.1016/j.ijbiomac.2022.05.159

20. A protocol for the generation of Arachis hypogaea composite plants: A valuable tool for the functional study of mycorrhizal symbiosis

Author:
Kalpana Nanjareddy; Isaac Zepeda‐Jazo; Manoj‐Kumar Arthikala
Source:
Applications in plant sciences 2022 v.10 no.1 pp. e11454
ISSN:
2168-0450
Subject:
genetic vectors,   etc   ; Agrobacterium; Arachis hypogaea; cost effectiveness; genetically modified organisms; green fluorescent protein; legumes; root systems; rooting; vesicular arbuscular mycorrhizae;     Show all 10 Subjects
Abstract:
... PREMISE: Agrobacterium rhizogenes–induced hairy root systems are one of the most preferred and versatile systems for the functional characterization of genes. The use of hairy root systems is a rapid and convenient alternative for studying root biology, biotic and abiotic stresses, and root symbiosis in in vitro recalcitrant legume species such as Arachis hypogaea. METHODS AND RESULTS: We present ...
DOI:
10.1002/aps3.11454