PubAg

Main content area

You searched for: Subject "genotyping" Remove constraint Subject: "genotyping"
Start Over

Search

9,673  Search Results

« Previous | 1 - 20 of 9,673 |
Number of results to display per page

Search Results

1. Application of Toxoplasma gondii GRA15 peptides in diagnosis and serotyping

Author:
Li, Runli; Ma, Yeting; Li, Jin; Zhou, Penglai; Zheng, Fuguo; Liu, Qing; Gao, Wenwei
Source:
Microbial pathogenesis 2020 v.143 pp. 104168
ISSN:
0882-4010
Subject:
genotyping,   etc   ; Toxoplasma gondii; Western blotting; antibodies; genes; peptides; proteins; serodiagnosis;     Show all 8 Subjects
Abstract:
... Previous studies showed that three clonal strain types (I, II, and III) of Toxoplasma gondii can be distinguished using serotyping based on a series of polymorphic proteins. However, to establish a systematic serotyping method with higher resolution even being equal to that of genotyping, more specific peptide markers are needed. The objective of the present study was to determine the possibility ...
DOI:
10.1016/j.micpath.2020.104168

2. Application of hematoxylin reagent for sperm cell separation in sexual crime evidence

Author:
Kim, Joo-Young; Kim, Man Il; Lee, Hye Hyeon; Kim, Hye Lim; Lee, Eun-Jung; Lee, Yang-Han; Hwang, In-Kwan; Chun, Byung-Won; Kang, Pil-Won
Source:
Forensic science international 2020 v.307 pp. 110114
ISSN:
0379-0738
Subject:
genotyping,   etc   ; DNA; crime; forensic sciences; microsatellite repeats; spermatozoa; staining;     Show all 7 Subjects
Abstract:
... Seminal evidence obtained from a sexual crime scene provides clues for solving a case through forensic analysis. However, most evidence collected from sexual crime scenes is a mixture of sperm cells and vaginal discharge. Therefore, separating the sperm cells from the seminal evidence is very important. In this study, we developed a separation method for effectively separating sperm cells using di ...
DOI:
10.1016/j.forsciint.2019.110114

3. New high-sensitive rhAmp method for A1 allele detection in A2 milk samples

Author:
Giglioti, Rodrigo; Gutmanis, Gunta; Katiki, Luciana Morita; Okino, Cintia Hiromi; de Sena Oliveira, Márcia Cristina; Vercesi Filho, Anibal Eugênio
Source:
Food chemistry 2020 v.313 pp. 126167
ISSN:
0308-8146
Subject:
genotyping,   etc   ; DNA; alleles; animals; beta-casein; detection limit; gastrointestinal system; melting; milk; peptides; single nucleotide polymorphism;     Show all 11 Subjects
Abstract:
... Cows’ milk may contain two types of β-casein: A1 and A2. A1 digestion is associated with the release of β-casomorphine-7 peptide, which can cause adverse gastrointestinal effects. Two methods – high-resolution melting (HRM) and rhAmp® SNP genotyping – were developed to identify the β-casein gene (CSN2) A1 and A2 alleles directly in milk. DNA milk samples from 45 animals were examined and 10 sample ...
DOI:
10.1016/j.foodchem.2020.126167

4. Single-nucleotide polymorphism (SNP) genotyping assays for the varietal authentication of ‘Nebbiolo’ musts and wines

Author:
Boccacci, Paolo; Chitarra, Walter; Schneider, Anna; Rolle, Luca; Gambino, Giorgio
Source:
Food chemistry 2020 v.312 pp. 126100
ISSN:
0308-8146
Subject:
genotyping,   etc   ; DNA; Vitis vinifera; databases; fraud; genotype; polymerase chain reaction; red wines; single nucleotide polymorphism; winemaking;     Show all 10 Subjects
Abstract:
... ‘Nebbiolo’ (Vitis vinifera L.) is renowned for its use in producing monovarietal high-quality red wines, such Barolo and Barbaresco. The fight against fraud to safeguard high-quality productions requires an effective varietal identification system applicable in musts and wines. ‘Nebbiolo’-specific single-nucleotide polymorphisms (SNPs) were identified starting from available databases and 260 geno ...
DOI:
10.1016/j.foodchem.2019.126100

5. A novel high-resolution melting analysis-based method for Salmonella genotyping

Author:
Hu, Miaomiao; Yang, Dong; Wu, Xiaoli; Luo, Meng; Xu, Feng
Source:
Journal of microbiological methods 2020 v.172 pp. 105806
ISSN:
0167-7012
Subject:
genotyping,   etc   ; DNA; Salmonella; bacteria; butanol; dried milk; food safety; melting; salmonellosis; temperature;     Show all 10 Subjects
Abstract:
... To establish a simple and rapid high-resolution melting curve (HRM) method, 5 different strains of Salmonella were identified by adding DNA denaturants at different concentrations into the HRM system to change the characteristics of DNA melting and to obtain different Tm (dissolving temperature) values of DNA from different target bacteria. When the concentration of n-butanol was 7% (v/v), the Tm ...
DOI:
10.1016/j.mimet.2019.105806

6. A review of likelihood ratios in forensic science based on a critique of Stiffelman “No longer the Gold standard: Probabilistic genotyping is changing the nature of DNA evidence in criminal trials”

Author:
Buckleton, John; Robertson, Bernard; Curran, James; Berger, Charles; Taylor, Duncan; Bright, Jo-Anne; Hicks, Tacha; Gittelson, Simone; Evett, Ian; Pugh, Simone; Jackson, Graham; Kelly, Hannah; Kalafut, Tim; Bieber, Frederick R.
Source:
Forensic science international 2020 v.310 pp. 110251
ISSN:
0379-0738
Subject:
genotyping,   etc   ; Bayesian theory; DNA; computer software; decision making; forensic sciences; probability; scientists;     Show all 8 Subjects
Abstract:
... Stiffelman [1] gives a broad critique of the application of likelihood ratios (LRs) in forensic science, in particular their use in probabilistic genotyping (PG) software. These are discussed in this review.LRs do not infringe on the ultimate issue. The Bayesian paradigm clearly separates the role of the scientist from that of the decision makers and distances the scientist from comment on the ult ...
DOI:
10.1016/j.forsciint.2020.110251

7. Mitochondrial Profiles of the East Bulgarian and the Pleven Horse Breeds

Author:
Hristov, Peter; Yordanov, Georgi; Vladov, Vladimir; Neov, Boyko; Palova, Nadezhda; Radoslavov, Georgi
Source:
Journal of equine veterinary science 2020 v.88 pp. 102933
ISSN:
0737-0806
Subject:
genotyping,   etc   ; cultural heritage; genetic profile; genetic structure; horse breeding; horse breeds; horses; mitochondria; Bulgaria; Middle East;     Show all 10 Subjects
Abstract:
... It is well known that horse breeding in Bulgaria is a cultural heritage in Bulgaria, dating from prehistoric and historic times. Until now, molecular data on Bulgarian horses from the plain regions of the country were not available. Therefore, for the first time, we have collected genetic information about some modern horse breeds from the plain regions in Bulgaria. A total of 50 horses originatin ...
DOI:
10.1016/j.jevs.2020.102933

8. Opinion: Is gene mapping in wild populations useful for understanding and predicting adaptation to global change?

Author:
Gienapp, Phillip
Source:
Global change biology 2020 v.26 no.5 pp. 2737-2749
ISSN:
1354-1013
Subject:
genotyping,   etc   ; biodiversity; chromosome mapping; climate change; environmental factors; extinction; genomics; prediction;     Show all 8 Subjects
Abstract:
... Changing environmental conditions will inevitably alter selection pressures. Over the long term, populations have to adapt to these altered conditions by evolutionary change to avoid extinction. Quantifying the ‘evolutionary potential’ of populations to predict whether they will be able to adapt fast enough to forecasted changes is crucial to fully assess the threat for biodiversity posed by clima ...
DOI:
10.1111/gcb.15058

9. Warmblood fragile foal syndrome type 1 mutation (PLOD1 c.2032G>A) is not associated with catastrophic breakdown and has a low allele frequency in the Thoroughbred breed

Author:
Bellone, R. R.; Ocampo, N. R.; Hughes, S. S.; Le, V.; Arthur, R.; Finno, C. J.; Penedo, M. C. T.
Source:
Equine veterinary journal 2020 v.52 no.3 pp. 411-414
ISSN:
0425-1644
Subject:
genotyping,   etc   ; DNA; Thoroughbred; alleles; collagen; crosslinking; death; foals; gene frequency; homozygosity; mutation; racehorses; risk factors; screening;     Show all 14 Subjects
Abstract:
... BACKGROUND: Catastrophic fractures are among the most common cause of fatalities in racehorses. Several factors, including genetics, likely contribute to increased risk for fatal injuries. A variant in the procollagen‐lysine, 2‐oxoglutarate 5‐dioxygenase1 gene (PLOD1 c.2032G>A) was shown to cause Warmblood fragile foal syndrome type 1 (WFFS), a fatal recessive defect of the connective tissue. Scre ...
DOI:
10.1111/evj.13182
PubMed:
31502696
PubMed Central:
PMC7062577

10. Validation of the Microreader™ 29Y Prime ID system for forensic use

Author:
Zhao, Yi; Zhang, Juanceng; He, Ti; Chen, Chengjian; Guo, Dan; Jiao, Zhangping; Yang, Zhipan; Yu, Zailiang; Ma, Wanshan; Zhang, Qingxia
Source:
Forensic science international 2020 v.309 pp. 110147
ISSN:
0379-0738
Subject:
genotyping,   etc   ; DNA; forensic sciences; guidelines; inheritance (genetics); loci; males; paternity; polymerase chain reaction;     Show all 9 Subjects
Abstract:
... Currently, Y-short tandem repeat loci (Y-STRs) have been increasingly used in the forensic field, particularly in investigations of sexual assault, determination of paternity and male lineage studies because of the characteristics of male-only and paternal inheritance. The Microreader™ 29Y Prime ID system is a 29-plex Y-STR genotyping system that amplifies 17 widely used commercial loci (DYS570, D ...
DOI:
10.1016/j.forsciint.2020.110147

11. Different filtering strategies of genotyping‐by‐sequencing data provide complementary resolutions of species boundaries and relationships in a clade of sexually deceptive orchids

Author:
Cozzolino, Salvatore; Scopece, Giovanni; Roma, Luca; Schlüter, Philipp M.
Source:
Journal of systematics and evolution 2020 v.58 no.2 pp. 133-144
ISSN:
1674-4918
Subject:
genotyping by sequencing,   etc   ; DNA; Ophrys; biological speciation; data collection; haplotypes; homozygosity; hybridization; loci; nuclear genome; phylogeny;     Show all 11 Subjects
Abstract:
... Ongoing hybridization and retained ancestral polymorphism in rapidly radiating lineages could mask recent cladogenetic events. This presents a challenge for the application of molecular phylogenetic methods to resolve differences between closely related taxa. We reanalyzed published genotyping‐by‐sequencing (GBS) data to infer the phylogeny of four species within the Ophrys sphegodes complex, a re ...
DOI:
10.1111/jse.12493

12. Polycyclic aromatic hydrocarbons exposure and their joint effects with age, smoking, and TCL1A variants on mosaic loss of chromosome Y among coke-oven workers

Author:
Liu, Yuhang; Bai, Yansen; Wu, Xiulong; Li, Guyanan; Wei, Wei; Fu, Wenshan; Wang, Gege; Feng, Yue; Meng, Hua; Li, Hang; Li, Mengying; Guan, Xin; Zhang, Xiaomin; He, Meian; Wu, Tangchun; Guo, Huan
Source:
Environmental pollution 2020 v.258 pp. 113655
ISSN:
0269-7491
Subject:
genotyping,   etc   ; alleles; blood; chromosomes; genetic variation; genotype; males; metabolites; mortality; pollution; polycyclic aromatic hydrocarbons; risk factors;     Show all 12 Subjects
Abstract:
... Mosaic loss of chromosome Y (mLOY) is the most common structure somatic event that related to increased risks of various diseases and mortality. Environmental pollution and genetic susceptibility were important contributors to mLOY. We aimed to explore the associations of polycyclic aromatic hydrocarbons (PAHs) exposure, as well as their joint effects with age, smoking, and genetic variants on per ...
DOI:
10.1016/j.envpol.2019.113655

13. The first evidence of an association between a polymorphism in the endocannabinoid-degrading enzyme FAAH (FAAH rs2295633) with attention deficit hyperactivity disorder

Author:
Ahmadalipour, Ali; Mehdizadeh Fanid, Leila; Zeinalzadeh, Narges; Alizadeh, Maedeh; Vaezi, Helaleh; Hassanpour Aydinlou, Zahra; Noorazar, Seyed Gholamreza
Source:
Genomics 2020 v.112 no.2 pp. 1330-1334
ISSN:
0888-7543
Subject:
genotyping,   etc   ; alleles; amide hydrolases; cannabinoids; children; fatty acids; gene frequency; genotype; risk; single nucleotide polymorphism;     Show all 10 Subjects
Abstract:
... Several single nucleotide polymorphisms (SNPs) of the fatty acid amide hydrolase (FAAH), the degrading enzyme of the endocannabinoids, have been shown to be associated with many neuropsychiatric disorders. Here, FAAH rs2295633 was studied in ADHD and case-control healthy children. There was a significant difference in the allele frequency (P = .04) and genotype distribution (P = .04) of the FAAH r ...
DOI:
10.1016/j.ygeno.2019.07.024

14. Genetic variability of Polish serogroup B meningococci (2010–2016) including the 4CMenB vaccine component genes

Author:
Waśko, Izabela; Gołębiewska, Agnieszka; Kiedrowska, Marlena; Ronkiewicz, Patrycja; Wróbel-Pawelczyk, Izabela; Kuch, Alicja; Hong, Eva; Skoczyńska, Anna
Source:
Vaccine 2020 v.38 no.8 pp. 1943-1952
ISSN:
0264-410X
Subject:
genotyping,   etc   ; Neisseria meningitidis; alleles; antigens; genetic variation; peptides; proteins; serotypes; vaccines; Poland;     Show all 10 Subjects
Abstract:
... Neisseria meningitidis serogroup B (MenB) has recently become the major cause of invasive meningococcal disease in Poland. Therefore, the purpose of this study was to characterize MenB isolates, responsible for invasive meningococcal disease in 2010–2016, by MLST and sequencing of genes encoding proteins used as 4CMenB vaccine antigens. Two methods of coverage estimation were performed: extrapolat ...
DOI:
10.1016/j.vaccine.2020.01.021

15. Molecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in Poland

Author:
Krajewska-Wędzina, Monika; Kozińska, Monika; Radulski, Łukasz; Lipiec, Marek; Augustynowicz-Kopeć, Ewa; Weiner, Marcin; Szulowski, Krzysztof
Source:
Journal of Veterinary Research 2020 v.64 no.1 pp. 45-50
ISSN:
2450-8608
Subject:
genotyping,   etc   ; Mycobacterium bovis; bovine tuberculosis; cattle; farms; herds; laboratory experimentation; phylogeny; zoonoses; Poland;     Show all 10 Subjects
Abstract:
... Since 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there were from 8 to 18 outbreaks of the disease. In 2008–2016, the largest number of cattle infected with bovine mycobacteria were eliminated in the Masovian Province (the central region of Poland) and the largest number of outbreaks of this zoonosis were record ...
DOI:
10.2478/jvetres-2020-0003
PubMed:
32258799
PubMed Central:
PMC7105984

16. Equine fetal genotyping via aspiration of yolk-sac fluid at 22–28 days of gestation

Author:
Ripley, A.M.; Penedo, M.C.T.; Grahn, R.A.; Martinez de Andino, E.V.; Walbornn, S.R.; Serafini, R.; Love, C.C.; Hinrichs, K.
Source:
Theriogenology 2020 v.142 pp. 34-40
ISSN:
0093-691X
Subject:
genotyping,   etc   ; DNA; alleles; biopsy; endometrium; foals; fractionation; genotype; loci; mares; polymerase chain reaction; pregnancy; yolk sac;     Show all 13 Subjects
Abstract:
... Fetal genotyping has important applications in the horse, but currently necessitates embryo recovery and biopsy. We investigated whether fetal genotyping could be performed on yolk-sac fluid recovered from pregnant mares via transvaginal aspiration. Fluid was collected before Day 30 to provide results before establishment of the endometrial cups (Day 37). Genotyping and assessment of maternal DNA ...
DOI:
10.1016/j.theriogenology.2019.09.012

17. Genetic polymorphism of angiotensin converting enzyme and angiotensin II type 1 receptors and their impact on the outcome of acute coronary syndrome

Author:
Ghafil, Fadhaa A.; Mohammad, Bassim I.; Al-Janabi, Hussain S.; Hadi, Najah R.; Al-Aubaidy, Hayder A.
Source:
Genomics 2020 v.112 no.1 pp. 867-872
ISSN:
0888-7543
Subject:
genotyping,   etc   ; angiotensin II; genetic polymorphism; genotype; patients; peptidyl-dipeptidase A; phenotype; receptors; risk;     Show all 9 Subjects
Abstract:
... This study aims to investigate the impact of ACE (rs4343) and AT1R (rs 5182) genetic polymorphisms on the outcome of acute coronary syndrome (ACS) in patients on captopril. Two hundred and fifty participants with ACS were included in this study (Group 1 (120) participants on captopril 25 mg twice daily and Group 2 (130) participants received no captopril (control study)). Participants were genotyp ...
DOI:
10.1016/j.ygeno.2019.05.028

18. Genotyping Neisseria gonorrhoeae gyrA and penA antimicrobial genes from remnant Neisseria gonorrhoeae positive Cepheid Xpert® clinical specimens – A feasibility study

Author:
Qadir, S.; Ellis, O.; Keizur, E.M.; Stafylis, C.; Cortado, R.; Klausner, J.D.
Source:
Journal of microbiological methods 2020 v.168 pp. 105783
ISSN:
0167-7012
Subject:
genotyping,   etc   ; DNA topoisomerase (ATP-hydrolysing); Neisseria gonorrhoeae; antibiotics; feasibility studies; genes; serine;     Show all 7 Subjects
Abstract:
... Neisseria gonorrhoeae (NG) has developed resistance to most antibiotics, making it increasingly difficult to treat. Previous studies have predicted antimicrobial NG susceptibility based on the antimicrobial gene target DNA gyrase subunit A (gyrA) codon serine 91 and the penicillin-binding protein 2 (penA) using Roche Cobas® and Hologic APTIMA™ clinical specimens. We studied whether similar methods ...
DOI:
10.1016/j.mimet.2019.105783

19. In silico quality assessment of SNPs—A case study on the Axiom® Wheat genotyping arrays

Author:
Lange, Thomas M.; Heinrich, Felix; Enders, Matthias; Wolf, Markus; Schmitt, Armin O.
Source:
Current plant biology 2020 v.21 pp. 100140
ISSN:
2214-6628
Subject:
genotyping,   etc   ; Triticum aestivum; case studies; computer simulation; computer software; databases; genome; genomics; single nucleotide polymorphism; wheat;     Show all 10 Subjects
Abstract:
... Genotyping arrays proved to be an exemplary tool for the simultaneous analysis of a multitude of single nucleotide polymorphisms (SNPs), a special case of genomic variants. By the example of SNPs represented on the Axiom® Wheat HD genotyping array as well as on the Axiom® Wheat Breeder's genotyping array, we applied a three way classification system to assess the quality of SNPs in bread wheat (Tr ...
DOI:
10.1016/j.cpb.2020.100140

20. Short communication: Lethal mutations in Vorderwald cattle through Montbéliarde incrossings

Author:
Reinartz, S.; Distl, O.
Source:
Journal of dairy science 2020 v.103 no.1 pp. 613-618
ISSN:
0022-0302
Subject:
genotyping,   etc   ; Simmental; alleles; bulls; fetus; gene frequency; haplotypes; heterozygosity; homozygosity; introgression; loci; mutants; mutation;     Show all 13 Subjects
Abstract:
... Vorderwald cattle are a dual-purpose cattle breed with high migrant contributions from Montbéliarde bulls in the recent past. Through the wide use of Montbéliarde bulls, undesirable alleles were also disseminated into the Vorderwald population. Haplotypes on bovine chromosome 19 (MH1) and 29 (MH2), supposed to harbor lethal mutations, were identified in Montbéliarde cattle. A study in French Montb ...
DOI:
10.3168/jds.2019-17213