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- Author:
- Perrone, Francesca; Craparo, Emanuela Fabiola; Cemazar, Maja; Kamensek, Urska; Drago, Salvatore Emanuele; Dapas, Barbara; Scaggiante, Bruna; Zanconati, Fabrizio; Bonazza, Debora; Grassi, Mario; Truong, Nhung; Pozzato, Gabriele; Farra, Rossella; Cavallaro, Gennara; Grassi, Gabriele
- Source:
- Journal of controlled release 2021 v.330 pp. 1132-1151
- ISSN:
- 0168-3659
- Subject:
- green fluorescent protein, etc ; animal models; composite polymers; galactose; gene expression regulation; gene overexpression; genes; hepatocytes; hepatoma; humans; messenger RNA; mice; polyethylene glycol; small interfering RNA; toxicity; xenotransplantation; Show all 16 Subjects
- Abstract:
- ... Given the lack of effective treatments for Hepatocellular carcinoma (HCC), the development of novel therapeutic approaches is very urgent. Here, siRNAs were delivered to HCC cells by a synthetic polymer containing α,β-poly-(N-2-hydroxyethyl)-D,L-aspartamide-(PHEA) derivatized with diethylene triamine (DETA) and bearing in the side chain galactose (GAL) linked via a polyethylene glycol (PEG) to obt ...
- DOI:
- 10.1016/j.jconrel.2020.11.020
-
https://dx.doi.org/10.1016/j.jconrel.2020.11.020
- Author:
- Xu, Jingyi; Su, Liyun; Han, Jinyan; Gao, Kaimin; Zhang, Miaomiao; Wang, Shuo; Chen, Chaoxiang; Yan, Xiaomei
- Source:
- Talanta 2021 v.222 pp. 121523
- ISSN:
- 0039-9140
- Subject:
- green fluorescent protein, etc ; antimycin A; apoptosis; betulinic acid; cancer therapy; cytosol; fluorescence; genomics; in vitro studies; membrane potential; mitochondria; neoplasm progression; neoplasms; red fluorescent protein; Show all 14 Subjects
- Abstract:
- ... Mitochondrial fusion is essential to maintain genomic stability and physiological functions of mitochondria. Since mitochondrial fusion and fission work in concert to regulate mitochondrial morphology and functions, it has been challenging to quantitatively measure the direct roles of mitochondrial fusion in apoptosis and cancer progression. Here, we report the development of a high-throughput in ...
- DOI:
- 10.1016/j.talanta.2020.121523
-
https://dx.doi.org/10.1016/j.talanta.2020.121523
- Author:
- Yang, Linjiang; Hu, Xixia; Cheng, Anchun; Wang, Mingshu; Jia, Renyong; Yang, Qiao; Wu, Ying; Chen, Shun; Liu, Mafeng; Zhu, Dekang; Ou, Xumin; Wen, XingJian; Mao, Sai; Sun, Di; Zhang, Shaqiu; Zhao, Xinxin; Huang, Juan; Gao, Qun; Liu, Yunya; Yu, Yanling; Zhang, Ling; Tian, Bin; Pan, Leichang; Chen, Xiaoyue
- Source:
- Poultry science 2021 v.100 no.1 pp. 26-38
- ISSN:
- 0032-5791
- Subject:
- green fluorescent protein, etc ; Anatid alphaherpesvirus 1; amino acids; cell culture; importins; ivermectin; mutants; nuclear localization signals; physiological transport; poultry diseases; protein kinases; transfection; virus replication; Show all 13 Subjects
- Abstract:
- ... Duck enteritis virus (DEV) multifunctional tegument protein UL13 is predicted to be a conserved herpesvirus protein kinase; however, little is known about its subcellular localization signal. In this study, through transfection of 2 predicted nuclear signals of DEV UL13 fused to enhanced green fluorescent protein, 2 bipartite nuclear localization signals (NLS) were identified. We found that iverme ...
- DOI:
- 10.1016/j.psj.2020.09.069
-
https://dx.doi.org/10.1016/j.psj.2020.09.069
- Author:
- Zappi, Daniele; Coronado, Edith; Soljan, Vice; Basile, Giovanni; Varani, Gabriele; Turemis, Mehmet; Giardi, Maria Teresa
- Source:
- Talanta 2021 v.221 pp. 121438
- ISSN:
- 0039-9140
- Subject:
- green fluorescent protein, etc ; Escherichia coli; Nitrosomonas europaea; ammonia; bioassays; bioluminescence; fluorescence; mercury; monitoring; nitrification; nitrogen; oxidation; phenol; promoter regions; reporter genes; toxic substances; toxicity; wastewater; wastewater treatment; water quality; Show all 20 Subjects
- Abstract:
- ... To avoid the upset of nitrification process in wastewater treatment plants, monitoring of influent toxic chemicals is essential for stable operation. Toxic chemical compounds can interfere with the biological nitrogen removal, thus affecting plant efficiency and effluent water quality. Here we report the development of fluorescence and bioluminescence bioassays, based on E. coli engineered to cont ...
- DOI:
- 10.1016/j.talanta.2020.121438
-
https://dx.doi.org/10.1016/j.talanta.2020.121438
- Author:
- Markus, Miles B.
- Source:
- Trends in parasitology 2020 v.36 no.5 pp. 407-408
- ISSN:
- 1471-4922
- Subject:
- green fluorescent protein, etc ; Plasmodium; humans; malaria; relapse; schizonts; Show all 6 Subjects
- Abstract:
- ... The progression to schizont formation of individual activated hypnozoites has been observed in vitro for the first time by Voorberg-van der Wel et al. Green-fluorescent protein-positive hypnozoites turned red-fluorescent (mCherry) upon activation. Thus, we now have empirical parasitological proof that supports the 40-year-old hypnozoite theory of relapse in malaria. ...
- DOI:
- 10.1016/j.pt.2020.01.011
-
https://dx.doi.org/10.1016/j.pt.2020.01.011
- Author:
- Marter, Pia; Schmidt, Sebastian; Kiontke, Stephan; Moog, Daniel
- Source:
- Protist 2020 v.171 no.1 pp. 125715
- ISSN:
- 1434-4610
- Subject:
- green fluorescent protein, etc ; Phaeodactylum tricornutum; fluorescence; genes; microalgae; models; plastids; Show all 7 Subjects
- Abstract:
- ... Phaeodactylum tricornutum is an ecologically and evolutionarily relevant microalga that has developed into an important model for molecular biological studies on organisms with complex plastids. The diatom is particularly suitable for in vivo protein localization analyses via fluorescence microscopy in which the green fluorescent protein (GFP) and its derivatives are dominantly used. Whereas GFP f ...
- DOI:
- 10.1016/j.protis.2020.125715
-
https://dx.doi.org/10.1016/j.protis.2020.125715
- Author:
- Thomson, Nicholas M.; Zhang, Chuanzhen; Trampari, Eleftheria; Pallen, Mark J.
- Source:
- BMC biotechnology 2020 v.20 no.1 pp. 54
- ISSN:
- 1472-6750
- Subject:
- green fluorescent protein, etc ; DNA fragmentation; Escherichia coli; biotechnology; chromosomes; gene editing; genes; mutagenesis; plasmids; screening; suicide; Show all 11 Subjects
- Abstract:
- ... BACKGROUND: Gene doctoring is an efficient recombination-based genetic engineering approach to mutagenesis of the bacterial chromosome that combines the λ-Red recombination system with a suicide donor plasmid that is cleaved in vivo to generate linear DNA fragments suitable for recombination. The use of a suicide donor plasmid makes Gene Doctoring more efficient than other recombineering technolog ...
- DOI:
- 10.1186/s12896-020-00648-5
-
https://dx.doi.org/10.1186/s12896-020-00648-5
- Author:
- Sato, R.; Minamihata, K.; Wakabayashi, R.; Goto, M.; Kamiya, N.
- Source:
- Analytical biochemistry 2020 v.600 pp. 113700
- ISSN:
- 0003-2697
- Subject:
- green fluorescent protein, etc ; catalytic activity; crosslinking; molecular weight; peptides; protein-glutamine gamma-glutamyltransferase; site-directed mutagenesis; Show all 7 Subjects
- Abstract:
- ... Assembling proteins in close vicinity to each other provides an opportunity to gain unique function because collaborative and even synergistic functionalities can be expected in an assembled form. There have been a variety of strategies to synthesize functional protein assemblies but site-specific covalent assembly of monomeric protein units without impairing their intrinsic function remains chall ...
- DOI:
- 10.1016/j.ab.2020.113700
-
https://dx.doi.org/10.1016/j.ab.2020.113700
- Author:
- Lin, Chi-Yun; Boxer, Steven G.
- Source:
- Journal of the American Chemical Society 2020 v.142 no.25 pp. 11032-11041
- ISSN:
- 1520-5126
- Subject:
- green fluorescent protein, etc ; color; dyes; image analysis; models; mutants; protonation; site-directed mutagenesis; spectroscopy; zwitterions; Show all 10 Subjects
- Abstract:
- ... The neutral or A state of the green fluorescent protein (GFP) chromophore is a remarkable example of a photoacid naturally embedded in the protein environment and accounts for the large Stokes shift of GFP in response to near UV excitation. Its color tuning mechanism has been largely overlooked, as it is less preferred for imaging applications than the redder anionic or B state. Past studies, base ...
- DOI:
- 10.1021/jacs.0c02796
-
http://dx.doi.org/10.1021/jacs.0c02796
- Author:
- Ko, Sanghwan; Jeon, Hyunwoo; Yoon, Sanghan; Kyung, Munsu; Yun, Hyungdon; Na, Jung-Hyun; Jung, Sang Taek
- Source:
- Journal of agricultural and food chemistry 2020 v.68 no.21 pp. 5873-5879
- ISSN:
- 1520-5118
- Subject:
- green fluorescent protein, etc ; Pseudomonas putida; bacteria; fluorescence; gastrointestinal system; humans; reducing agents; screening; Show all 8 Subjects
- Abstract:
- ... Oxygen-independent, flavin-binding fluorescent proteins (FbFPs) are emerging as alternatives to green fluorescent protein (GFP), which has limited applicability in studying anaerobic microorganisms, such as human gastrointestinal bacteria, which grow in oxygen-deficient environments. However, the utility of these FbFPs has been compromised because of their poor fluorescence emission. To overcome t ...
- DOI:
- 10.1021/acs.jafc.0c00121
-
http://dx.doi.org/10.1021/acs.jafc.0c00121
- Author:
- Figg, C. Adrian; Winegar, Peter H.; Hayes, Oliver G.; Mirkin, Chad A.
- Source:
- Journal of the American Chemical Society 2020 v.142 no.19 pp. 8596-8601
- ISSN:
- 1520-5126
- Subject:
- green fluorescent protein, etc ; DNA; base pair mismatch; hybridization chain reaction; mutants; oligomerization; polymerization; protein subunits; Show all 8 Subjects
- Abstract:
- ... A novel method for controlling the oligomerization of metastable DNA hairpins using the hybridization chain reaction (HCR) is reported. Control was achieved through the introduction of a base-pair mismatch in the duplex of the hairpins. The mismatch modification allows one to kinetically differentiate initiation versus propagation events, leading to DNA oligomers up to 10 monomers long and improvi ...
- DOI:
- 10.1021/jacs.0c02892
-
http://dx.doi.org/10.1021/jacs.0c02892
- Author:
- Singh, Ashish; Verma, Parul; Laha, Subhajit; Samanta, Debabrata; Roy, Syamantak; Maji, Tapas Kumar
- Source:
- ACS applied materials & interfaces 2020 v.12 no.18 pp. 20991-20997
- ISSN:
- 1944-8252
- Subject:
- green fluorescent protein, etc ; aldehydes; encapsulation; heat treatment; irradiation; isomers; polymers; porosity; porous media; ultraviolet radiation; Show all 10 Subjects
- Abstract:
- ... Design and synthesis of solid-state photochromic materials remain a challenge because of high structural constrain. However, this can be mitigated in attaining structural flexibility by introducing permanent porosity into the system. Here, we report for the first time the design and synthesis of a photochromic conjugated microporous polymer (pcCMP) by assembling photochromic dithienylethene aldehy ...
- DOI:
- 10.1021/acsami.0c05182
-
http://dx.doi.org/10.1021/acsami.0c05182
- Author:
- Yang, Hongyi; Zhao, Xingyu; Li, Lili; Zhang, Jie
- Source:
- Plant methods 2020 v.16 no.1 pp. 102
- ISSN:
- 1746-4811
- Subject:
- green fluorescent protein, etc ; Agrobacterium radiobacter; Cryptosporiopsis; Sordariomycetes; Vaccinium uliginosum; fluorescence; genes; mineral cycle; mycorrhizal fungi; polymerase chain reaction; roots; Show all 11 Subjects
- Abstract:
- ... BACKGROUND: Ericoid mycorrhizal fungi (EMF) play important roles in mineral cycling and plant nutrient acquisition, and they increase plant survival in nutrient-poor environments. In this study, we detected the colonization of EMF using a green fluorescent protein (GFP) expression method and in situ PCR. RESULTS: Genetic transformants of Cryptosporiopsis ericae and Sordariomycetes sp. expressing G ...
- DOI:
- 10.1186/s13007-020-00645-x
-
https://dx.doi.org/10.1186/s13007-020-00645-x
- Author:
- Zhong, Zhaohui; Liu, Shishi; Liu, Xiaopei; Liu, Binglin; Tang, Xu; Ren, Qiurong; Zhou, Jianping; Zheng, Xuelian; Qi, Yiping; Zhang, Yong
- Source:
- Rice 2020 v.13 no.1 pp. 8
- ISSN:
- 1939-8425
- Subject:
- green fluorescent protein, etc ; CRISPR-Cas systems; RNA; engineering; gene editing; introns; light; open reading frames; plant biology; rice; slicing; testing; Show all 12 Subjects
- Abstract:
- ... BACKGROUND: Expression of either Cas9 or Cas12a and guide RNAs by a single Polymerase II (Pol II) promoter represents a compact CRISPR expression system and has many advantages for different applications. In order to make this system routine in plant biology, engineering efforts are needed for developing and optimizing such single transcript unit (STU) systems for plant genome editing. RESULTS: To ...
- DOI:
- 10.1186/s12284-020-0369-8
-
https://dx.doi.org/10.1186/s12284-020-0369-8
- Author:
- Tokumoto, Shoko; Miyata, Yugo; Usui, Kengo; Deviatiiarov, Ruslan; Ohkawa, Takahiro; Kondratieva, Sabina; Shagimardanova, Elena; Gusev, Oleg; Cornette, Richard; Itoh, Masayoshi; Hayashizaki, Yoshihide; Kikawada, Takahiro
- Source:
- Insects 2020 v.11 no.11
- ISSN:
- 2075-4450
- Subject:
- green fluorescent protein, etc ; Aequorea; Avian myeloblastosis virus; DNA; Polypedilum; RNA-directed DNA polymerase; catalytic activity; gene expression; luciferase; reporter genes; Show all 10 Subjects
- Abstract:
- ... The Pv11 cell line established from an African chironomid, Polypedilum vanderplanki, is the only cell line tolerant to complete desiccation. In Pv11 cells, a constitutive expression system for Pv11 cells was previously exploited and several reporter genes were successfully expressed. Here we report the identification of an effective minimal promoter for Pv11 cells and its application to the Tet-On ...
- DOI:
- 10.3390/insects11110781
-
http://dx.doi.org/10.3390/insects11110781
- Author:
- Carlos, Carlota P. A.; Correia, Sandra F. H.; Martins, Margarida; Savchuk, Oleksandr A.; Coutinho, João A. P.; André, Paulo S.; Nieder, Jana B.; Ventura, Sónia P. M.; Ferreira, Rute A. S.
- Source:
- Green chemistry 2020 v.22 no.15 pp. 4943-4951
- ISSN:
- 1463-9270
- Subject:
- green fluorescent protein, etc ; absorption; aqueous solutions; energy; green chemistry; liquids; luminescence; photostability; solar cells; solar collectors; urban areas; Show all 11 Subjects
- Abstract:
- ... Luminescent solar concentrators (LSCs) are a solution to overcome the mismatch between solar cell absorption and the solar spectrum, facilitating the integration of photovoltaic (PV) devices into the urban environment, since they can be incorporated in building façades and windows. Challenges include the search for environmentally friendly materials with chemical and optical stabilities. The enhan ...
- DOI:
- 10.1039/d0gc01742f
-
http://dx.doi.org/10.1039/d0gc01742f
- Author:
- Limoges, Marie; Frontino, Gina; Donnelly, Catherine
- Source:
- Food control 2020 v.114 pp. 107272
- ISSN:
- 0956-7135
- Subject:
- green fluorescent protein, etc ; Listeria innocua; Listeria monocytogenes; artisan cheese; biobased products; bricks; environmental monitoring; plastics; risk; stainless steel; wood; Show all 11 Subjects
- Abstract:
- ... Preventing Listeria contamination of artisan cheeses requires routine and effective environmental monitoring of product contact surfaces within the production environment. Consideration of the efficacy of environmental monitoring methods is essential when testing for the presence of Listeria spp. within the processing environment as a way to control the risk of cheese contamination. Four environme ...
- DOI:
- 10.1016/j.foodcont.2020.107272
-
https://dx.doi.org/10.1016/j.foodcont.2020.107272
- Author:
- Sathesh-Prabu, Chandran; Kim, Donghyuk; Lee, Sung Kuk
- Source:
- Bioresource technology 2020 v.309 pp. 123361
- ISSN:
- 0960-8524
- Subject:
- green fluorescent protein, etc ; Escherichia coli; Pseudomonas putida; biochemical pathways; biomass; fruits; gene expression; glucose; hydrolysates; lignocellulose; metabolic engineering; xylose; Show all 12 Subjects
- Abstract:
- ... A glucose-inducible gene expression system has been developed using HexR-Pzwf₁ of Pseudomonas putida to induce the metabolic pathways. Since the system is controlled by an Entner–Doudoroff pathway (EDP) intermediate, the EDP of Escherichia coli was activated by deleting pfkA and gntR genes. Growth experiment with green fluorescent protein as a reporter indicated that the induction of this system w ...
- DOI:
- 10.1016/j.biortech.2020.123361
-
https://dx.doi.org/10.1016/j.biortech.2020.123361
- Author:
- Li, Liangliang; Tan, Jiajin; Chen, Fengmao; Hao, Dejun
- Source:
- Journal of forestry research 2020 v.31 no.3 pp. 1025-1033
- ISSN:
- 1007-662X
- Subject:
- green fluorescent protein, etc ; Bacillus cereus; Pinus elliottii; Pinus massoniana; antibiotics; bacteria; bacterial colonization; endophytes; genes; nematicidal properties; plasmids; roots; stems; Show all 13 Subjects
- Abstract:
- ... Bacillus cereus NJSZ-13, an endophytic bacterium with nematicidal activity, was isolated from stems of healthy Pinus elliottii Engelm. Colonization of P. massoniana Lamb. by endophytic B. cereus was studied using scanning electron microscopy and confocal laser scanning microscopy. After the plasmid pGFP78 containing the green fluorescent protein (GFP) gene was transformed into the NJSZ-13 strain, ...
- DOI:
- 10.1007/s11676-018-0823-2
-
http://dx.doi.org/10.1007/s11676-018-0823-2
- Author:
- Thakare, Swapnil S.; Bansal, Navita; Vanchinathan, S.; Rama Prashat, G.; Krishnan, Veda; Sachdev, Archana; Praveen, Shelly; Vinutha, T.
- Source:
- Journal of plant biochemistry and biotechnology 2020 v.29 no.2 pp. 183-192
- ISSN:
- 0971-7811
- Subject:
- green fluorescent protein, etc ; CRISPR-Cas systems; Glycine max; assays; biotechnology; detection; gene editing; genes; leaves; plant biochemistry; transgenic plants; Show all 11 Subjects
- Abstract:
- ... The CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR—associated proteins 9) is simple and highly efficient technology applied to functional studies of genes and genetic crop improvement. In this study, we have demonstrated the utility of green fluorescent protein (GFP) marker to detect the targeting efficiency of gRNAs. As a proof of concept, Glycine max De-Etiolated 1 ...
- DOI:
- 10.1007/s13562-019-00540-0
-
https://dx.doi.org/10.1007/s13562-019-00540-0
