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1. GFP fluorescence: A few lesser-known nuggets that make it work

Author:
Sarkar, Parijat; Chattopadhyay, Amitabha
Source:
Journal of biosciences 2018 v.43 no.3 pp. 421-430
ISSN:
0250-5991
Subject:
green fluorescent protein,   etc   ; biomarkers; fluorescence;     Show all 3 Subjects
Abstract:
... Use of Green Fluorescent Protein (GFP) as a marker has revolutionized biological research in the last few decades. In this brief commentary, we reflect upon the success story of GFP and highlight a few lesser-known facets about GFP that add up to its usefulness. ...
DOI:
10.1007/s12038-018-9779-9

2. A simple fluorescent assay for the discovery of protein-protein interaction inhibitors

Author:
Al-Mugotir, Mona; Kolar, Carol; Vance, Krysten; Kelly, David L.; Natarajan, Amarnath; Borgstahl, Gloria E.O.
Source:
Analytical biochemistry 2019 v.569 pp. 46-52
ISSN:
0003-2697
Subject:
green fluorescent protein,   etc   ; fluorescence; protein-protein interactions; protocols; screening; therapeutics;     Show all 6 Subjects
Abstract:
... Due to the therapeutic potential of targeting protein-protein interactions (PPIs) there is a need for easily executed assays to perform high throughput screening (HTS) of inhibitors. We have developed and optimized an innovative and robust fluorescence-based assay for detecting PPI inhibitors, called FluorIA (Fluorescence-based protein-protein Interaction Assay). Targeting the PPI of RAD52 with re ...
DOI:
10.1016/j.ab.2019.01.010

3. Assessment of GFP Tag Position on Protein Localization and Growth Fitness in Yeast

Author:
Weill, Uri; Krieger, Gat; Avihou, Zohar; Milo, Ron; Schuldiner, Maya; Davidi, Dan
Source:
Journal of molecular biology 2019 v.431 no.3 pp. 636-641
ISSN:
0022-2836
Subject:
green fluorescent protein,   etc   ; molecular biology; protein tagging; yeasts;     Show all 4 Subjects
Abstract:
... While protein tags are ubiquitously utilized in molecular biology, they harbor the potential to interfere with functional traits of their fusion counterparts. Systematic evaluation of the effect of protein tags on function would promote accurate use of tags in experimental setups. Here we examine the effect of green fluorescent protein tagging at either the N or C terminus of budding yeast protein ...
DOI:
10.1016/j.jmb.2018.12.004

4. RNA-aptamers—As targeted inhibitors of protein functions in plants

Author:
Abdeeva, Inna A.; Maloshenok, Liliya G.; Pogorelko, Gennady V.; Mokrykova, Maria V.; Bruskin, Sergey A.
Source:
Journal of plant physiology 2019 v.232 pp. 127-129
ISSN:
0176-1617
Subject:
green fluorescent protein,   etc   ; abiotic stress; biotic stress; fluorescence; nucleotide aptamers;     Show all 5 Subjects
Abstract:
... The scope of RNA-aptamers application is becoming wider and has expanded beyond solely medical use. We propose the use of RNA-aptamers in plants to suppress the functions of individual proteins, thereby achieving resistance to various biotic and abiotic stresses. In current work we experimentally demonstrate the possibility of inhibiting protein activity in N. bentamiana plants by quenching the fl ...
DOI:
10.1016/j.jplph.2018.10.026

5. Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein

Author:
Svendsen, Annette; Kiefer, Hjalte V.; Pedersen, Henrik B.; Bochenkova, Anastasia V.; Andersen, Lars H.
Source:
Journal of the American Chemical Society 2017 v.139 no.25 pp. 8766-8771
ISSN:
1520-5126
Subject:
green fluorescent protein,   etc   ; fluorescence; spectroscopy;     Show all 3 Subjects
Abstract:
... Green fluorescent protein, GFP, has revolutionized biology, due to its use in bioimaging. It is widely accepted that the protein environment makes its chromophore fluoresce, whereas the fluorescence is completely lost when the native chromophore is taken out of GFP. By the use of a new femtosecond pump–probe scheme, based on time-resolved action spectroscopy, we demonstrate that the isolated depro ...
DOI:
10.1021/jacs.7b04987

6. A GFP-tagged version of the pseudorabies virus protein UL56 localizes to the Golgi and trans-Golgi network through a predicted C-terminal leucine-rich helix in transfected cells

Author:
Lyu, Chuang; Cai, Xuehui
Source:
Virology journal 2019 v.16 no.1 pp. 81
ISSN:
1743-422X
Subject:
green fluorescent protein,   etc   ; Suid alphaherpesvirus 1; alanine; leucine; mutagenesis; mutants; viral proteins; virulence;     Show all 8 Subjects
Abstract:
... BACKGROUND: Pseudorabies virus (PRV) protein UL56 (pUL56) has been implicated in viral dissemination and virulence in vivo. However, the properties of PRV pUL56 remain largely unknown. In the present study, we aim to investigate the subcellular localization of pUL56 and the underlying molecular basis in transfected cells. METHODS: Constructs of N-terminal green fluorescent protein (GFP) fused pUL5 ...
DOI:
10.1186/s12985-019-1191-z

7. Identification of cucurbit chlorotic yellows virus P4.9 as a possible movement protein

Author:
Wei, Ying; Shi, Yajuan; Han, Xaioyu; Chen, Siyu; Li, Honglian; Chen, Linlin; Sun, Bingjian; Shi, Yan
Source:
Virology journal 2019 v.16 no.1 pp. 82
ISSN:
1743-422X
Subject:
green fluorescent protein,   etc   ; Agrobacterium; Cucurbit chlorotic yellows virus; RNA; endoplasmic reticulum; fluorescence; genome; pathogenicity; plasmids; plasmodesmata;     Show all 10 Subjects
Abstract:
... BACKGROUND: Cucurbit chlorotic yellows virus (CCYV) is a bipartite cucurbit-infecting crinivirus within the family Closteroviridae. The crinivirus genome varies among genera. P4.9 is the first protein encoded by CCYV RNA2. P5, which is encoded by LIYV, is necessary for efficient viral infectivity in plants; however, it remains unknown whether CCYV P4.9 is involved in movement. FINDING: In this stu ...
DOI:
10.1186/s12985-019-1192-y

8. Heavy metal(loid) biosensor based on split-enhanced green fluorescent protein: development and characterization

Author:
Kim, Hyojin; Lee, Woonwoo; Yoon, Youngdae
Source:
Applied microbiology and biotechnology 2019 v.103 no.15 pp. 6345-6352
ISSN:
0175-7598
Subject:
green fluorescent protein,   etc   ; biosensors; cadmium; fluorescence; heavy metals; human health; mercury; metal ions; metalloids; soil amendments;     Show all 10 Subjects
Abstract:
... Heavy metal(loid)s such as Cd and Hg adversely affect human health and are therefore strictly regulated and monitored; however, their quantitation in the environment is usually performed by expensive and time-consuming instrumental analysis techniques, which necessitates the search for more practical alternatives. Herein, we prepare enhanced green fluorescent protein (eGFP)–based biomolecules for ...
DOI:
10.1007/s00253-019-09908-7

9. Identification of the anchoring protein SpoIIIJ for construction of the microbial cell surface display system in Bacillus spp.

Author:
Ding, Zundan; Guan, Feifei; Yu, Xiaoxia; Li, Qingbin; Wang, Qian; Tian, Jian; Wu, Ningfeng
Source:
International journal of biological macromolecules 2019 v.133 pp. 614-623
ISSN:
0141-8130
Subject:
green fluorescent protein,   etc   ; Bacillus subtilis; Gram-positive bacteria; aryldialkylphosphatase; binding proteins; bioinformatics; bioremediation; membrane proteins; pollution;     Show all 9 Subjects
Abstract:
... Microbial cell surface display technology is a powerful tool for displaying proteins on the surfaces of cells. However, few anchoring proteins can be employed for the display of target proteins on the cell surface of the environmentally benign Gram-positive bacterium Bacillus subtilis. In this study, bioinformatics tools were used to screen all of the encoded proteins of B. subtilis for potential ...
DOI:
10.1016/j.ijbiomac.2019.04.033

10. A rapid in situ fluorescence census for coral reef monitoring

Author:
Ramesh, C.H.; Koushik, S.; Shunmugaraj, T.; Ramana Murthy, M.V.
Source:
Regional studies in marine science 2019 v.28 pp. 100575
ISSN:
2352-4855
Subject:
green fluorescent protein,   etc   ; Scyphozoa; algae; coral reefs; corals; fluorescence; health status; marine science; monitoring; ultraviolet radiation;     Show all 10 Subjects
Abstract:
... Since the discovery of Green fluorescent protein (GFP) from jellyfish, many GFP-like marine fluorescent proteins have been unveiled and implicated in myriad of biomedical and toxicological research applications. However, fluorescence trait has not been implemented in the field applications so far. In this study, using a UV-blue light, we induced fluorescence of corals to identify the diseased and ...
DOI:
10.1016/j.rsma.2019.100575

11. Oligoarginine-Bearing Tandem Repeat Penetration-Accelerating Sequence Delivers Protein to Cytosol via Caveolae-Mediated Endocytosis

Author:
Okuda, Akiko; Tahara, Shinya; Hirose, Hisaaki; Takeuchi, Toshihide; Nakase, Ikuhiko; Ono, Atsushi; Takehashi, Masanori; Tanaka, Seigo; Futaki, Shiroh
Source:
Biomacromolecules 2019 v.20 no.5 pp. 1849-1859
ISSN:
1526-4602
Subject:
green fluorescent protein,   etc   ; actin; crosslinking; cytosol; endocytosis; genistein; immunoglobulin G; mixing; peptides; polymerization; small interfering RNA;     Show all 11 Subjects
Abstract:
... To facilitate the cytosolic delivery of larger molecules such as proteins, we developed a new cell-penetrating peptide sequence, named Pas2r12, consisting of a repeated Pas sequence (FFLIG-FFLIG) and d-dodeca-arginine (r12). This peptide significantly enhanced the cellular uptake and cytosolic release of enhanced green fluorescent protein and immunoglobulin G as cargos. We found that simply mixing ...
DOI:
10.1021/acs.biomac.8b01299

12. Co-culturing on dry filter paper significantly increased the efficiency of Agrobacterium-mediated transformations of maize immature embryos

Author:
Duan, Xueqing; Zheng, Liru; Sun, Jinhao; Liu, Wenbo; Wang, Wenqian; An, Hailong
Source:
Physiology and molecular biology of plants 2019 v.25 no.2 pp. 549-560
ISSN:
0971-5894
Subject:
green fluorescent protein,   etc   ; Agrobacterium radiobacter; callus; coculture; corn; corn ears; plasmids; pollination;     Show all 8 Subjects
Abstract:
... In the Agrobacterium tumefaciens-mediated transformations of maize immature embryos (IEs), the common co-culturing media used are MS or N6-based (MC). Here, we used a novel co-culturing method in which maize ‘Qi319’ IEs inoculated with Agrobacterium-harboring target vector were placed on dry filter paper (DC) in a petri dish. To compare the effects of the DC and MC co-culturing methods on transfor ...
DOI:
10.1007/s12298-018-00641-5

13. Visualizing fungicide action: an in vivo tool for rapid validation of fungicides with target location HOG pathway

Author:
Bohnert, Stefan; Neumann, Hendrik; Thines, Eckhard; Jacob, Stefan
Source:
Pest management science 2019 v.75 no.3 pp. 772-778
ISSN:
1526-498X
Subject:
green fluorescent protein,   etc   ; Magnaporthe oryzae; fludioxonil; fluorescence; fungi; histidine kinase; mutants; osmotic stress;     Show all 8 Subjects
Abstract:
... BACKGROUND: The mitogen‐activated protein kinase MoHog1p was fused with a green fluorescent protein (GFP) in the filamentous fungus Magnaporthe oryzae. The MoHOG1::GFP mutant was found to be an excellent tool visualizing in vivo fungicide‐dependent translocation of MoHog1p into the nucleus. Validation of pathway specificity was achieved by generating fluorescence‐labelled MoHog1p in the ΔMohik1 ‘l ...
DOI:
10.1002/ps.5177

14. Developing Polysulfide-Sensitive GFPs for Real-Time Analysis of Polysulfides in Live Cells and Subcellular Organelles

Author:
Hu, Xin; Li, Huanjie; Zhang, Xi; Chen, Zhigang; Zhao, Rui; Hou, Ningke; Liu, Jihua; Xun, Luying; Liu, Huaiwei
Source:
Analytical chemistry 2019 v.91 no.6 pp. 3893-3901
ISSN:
1520-6882
Subject:
green fluorescent protein,   etc   ; cysteine; developmental stages; homeostasis; in vivo studies; microorganisms; models; organelles;     Show all 8 Subjects
Abstract:
... Polysulfides are newly discovered cellular contents, and they are involved in multiple intracellular processes, including redox homeostasis and protein sulfhydration. The dynamic changes of polysulfides inside the cell are directly related to these processes. To monitor the intracellular dynamics and subcellular levels of polysulfides, we developed green-fluorescent-protein (GFP)-based probes that ...
DOI:
10.1021/acs.analchem.8b04634

15. Encapsulation of biomacromolecules by soaking and co-crystallization into porous protein crystals of hemocyanin

Author:
Hashimoto, Tsubasa; Ye, Yuxin; Matsuno, Asuka; Ohnishi, Yuki; Kitamura, Akira; Kinjo, Masataka; Abe, Satoshi; Ueno, Takafumi; Yao, Min; Ogawa, Tomohisa; Matsui, Takashi; Tanaka, Yoshikazu
Source:
Biochemical and biophysical research communications 2019 v.509 no.2 pp. 577-584
ISSN:
0006-291X
Subject:
green fluorescent protein,   etc   ; catalytic activity; confocal microscopy; crystals; encapsulation; ferritin; hemocyanin; molecular weight; photobleaching; phycoerythrin; soaking;     Show all 11 Subjects
Abstract:
... Encapsulation of guest molecules into the hollow spaces of crystals has been applied for a variety of purposes such as structure determination, separation, and catalysis of the guest. Although host−guest studies have been developed mainly in crystals of small molecules, those of biomacromolecules have recently been applied. In those reports, a huge hollow space in the protein crystal is commonly u ...
DOI:
10.1016/j.bbrc.2018.12.096

16. SLIM microscopy allows for visualization of DNA-containing liposomes designed for sperm-mediated gene transfer in cattle

Author:
Rubessa, Marcello; Lotti, Samantha N.; Kandel, Mikhail E.; Popescu, Gabriel; Wheeler, Mathew B.
Source:
Molecular biology reports 2019 v.46 no.1 pp. 695-703
ISSN:
0301-4851
Subject:
green fluorescent protein,   etc   ; DNA; cattle; freeze-thaw cycles; gene transfer; microscopy; quantitative polymerase chain reaction; spermatozoa;     Show all 8 Subjects
Abstract:
... Naked DNA has been shown to bind naturally to the sperm, a method called sperm-mediated gene transfer (SMGT). Based on these observations, we examined the efficiency of exogenous DNA binding to sperm using liposomes. In this experiment, we analyzed methods to select frozen-thawed bovine sperm, and evaluated the binding of exogenous DNA to those sperm. To determine the optimal selection method, we ...
DOI:
10.1007/s11033-018-4525-9

17. Functional Analysis of Three Rice Chloroplast Transit Peptides

Author:
He, Lei; Chen, Guang; Zhang, Sen; Qiu, Zhennan; Hu, Jiang; Zeng, Dali; Zhang, Guangheng; Dong, Guojun; Gao, Zhenyu; Ren, Deyong; Shen, Lan; Guo, Longbiao; Qian, Qian; Zhu, Li
Source:
Rice science 2019 v.26 no.1 pp. 11-20
ISSN:
1672-6308
Subject:
green fluorescent protein,   etc   ; Oryza sativa; chloroplast transit peptides; chloroplasts; metabolism; protoplasts; rice;     Show all 7 Subjects
Abstract:
... Chloroplast transit peptides (CTPs) can be used to transport non-chloroplastic proteins into the chloroplasts. Here, we studied the CTPs of three rice (Oryza sativa L.) chloroplast-localized proteins and found that their CTPs could be used to transport non-chloroplast-localized proteins into the chloroplasts. Fusion proteins lacking the CTP remained located in the cytoplasm. Furthermore, we constr ...
DOI:
10.1016/j.rsci.2018.12.001

18. Orientation Controlled Protein Nanocapsules by Enzymatic Removal of a Polymer Template

Author:
Lee, Chaeyeon; Hwang, Aran; Jose, Leeja; Park, Ji Hyun; Song, Jae Kwang; Shim, KyuHwan; An, Seong Soo A.; Paik, Hyun-jong
Source:
Biomacromolecules 2018 v.19 no.11 pp. 4219-4227
ISSN:
1526-4602
Subject:
green fluorescent protein,   etc   ; biocompatibility; crosslinking; nanocapsules; nanocarriers; nickel; polylactic acid;     Show all 7 Subjects
Abstract:
... Protein nanocapsules are potentially useful as functional nanocarriers because of their hollow structure and high biocompatibility and the intrinsic activity of their protein constituents. However, the development of a facile method for the preparation of oriented nanocapsules that retain their protein activity has been challenging. Here we describe the preparation of protein nanocapsules through ...
DOI:
10.1021/acs.biomac.8b00965

19. Synthesis and Properties of the p-Sulfonamide Analogue of the Green Fluorescent Protein (GFP) Chromophore: The Mimic of GFP Chromophore with Very Strong N–H Photoacid Strength

Author:
Chen, Yi-Hui; Sung, Robert; Sung, Kuangsen
Source:
Organic letters 2018 v.20 no.7 pp. 1768-1772
ISSN:
1523-7052
Subject:
green fluorescent protein,   etc   ; chemical reactions; chemical structure; dimethyl sulfoxide; solvents;     Show all 5 Subjects
Abstract:
... The para-sulfonamide analogue (p-TsABDI) of a green fluorescent protein (GFP) chromophore was synthesized to mimic the GFP chromophore. Its S₁ excited-state pKₐ* value in dimethylsulfoxide (DMSO) is −1.5, which is strong enough to partially protonate dipolar aprotic solvents and causes excited-state proton transfer (ESPT), so it can partially mimic the GFP chromophore to further study the ESPT-rel ...
DOI:
10.1021/acs.orglett.8b00257

20. Endosomal Escape and Delivery of CRISPR/Cas9 Genome Editing Machinery Enabled by Nanoscale Zeolitic Imidazolate Framework

Author:
Alsaiari, Shahad K.; Patil, Sachin; Alyami, Mram; Alamoudi, Kholod O.; Aleisa, Fajr A.; Merzaban, Jasmeen S.; Li, Mo; Khashab, Niveen M.
Source:
Journal of the American Chemical Society 2018 v.140 no.1 pp. 143-146
ISSN:
1520-5126
Subject:
green fluorescent protein,   etc   ; RNA; gene editing; genetic disorders; moieties;     Show all 5 Subjects
Abstract:
... CRISPR/Cas9 is a combined protein (Cas9) and an engineered single guide RNA (sgRNA) genome editing platform that offers revolutionary solutions to genetic diseases. It has, however, a double delivery problem owning to the large protein size and the highly charged RNA component. In this work, we report the first example of CRISPR/Cas9 encapsulated by nanoscale zeolitic imidazole frameworks (ZIFs) w ...
DOI:
10.1021/jacs.7b11754