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1. Mutation of conserved residues K329 and R330 on the surface of firefly luciferase: Effect on proteolytic degradation

Author:
Jarchi, Samaneh; Ataei, Farangis; Hosseinkhani, Saman
Source:
International journal of biological macromolecules 2018 v.115 pp. 7-330
ISSN:
0141-8130
Subject:
half life,   etc   ; Photinus pyralis; fluorescence; luciferase; mutagenesis; mutants; point mutation; proteolysis; trypsin;     Show all 9 Subjects
Abstract:
... Firefly luciferase is highly susceptible to proteolytic digestion that reduces its half-life and leads to loss in sensitivity. Due to the protease contamination in most in vitro and in vivo environments, it has interest to generate some mutations that may lead to improved susceptibility to digestion. Some important conserved residues (including K206, R213, R218, K329, R330 and R337) on accessible ...
DOI:
10.1016/j.ijbiomac.2018.04.050

2. Tissue-specific turnover rates of the nitrogen stable isotope as functions of time and growth in a cyprinid fish

Author:
Busst, Georgina M. A.; Britton, J. Robert
Source:
Hydrobiologia 2018 v.805 no.1 pp. 49-60
ISSN:
0018-8158
Subject:
half life,   etc   ; Barbus barbus; diet; fish; juveniles; muscles; nitrogen; stable isotopes; tissues;     Show all 9 Subjects
Abstract:
... Ecological applications of stable isotope data require knowledge on the isotopic turnover rate of tissues, usually described as the isotopic half-life in days (T ₀.₅) or the change in mass (G ₀.₅). Ecological studies increasingly analyse tissues collected non-destructively, such as fish fin and scales, but there is limited knowledge on their turnover rates. Determining turnover rates in situ is ch ...
DOI:
10.1007/s10750-017-3276-2

3. B-factor-saturation mutagenesis as a strategy to increase the thermostability of α-L-rhamnosidase from Aspergillus terreus

Author:
Ge, Lin; Li, Dongdong; Wu, Tao; Zhao, Linguo; Ding, Gang; Wang, Zhenzhong; Xiao, Wei
Source:
Journal of biotechnology 2018 v.275 pp. 7-23
ISSN:
0168-1656
Subject:
half life,   etc   ; Aspergillus terreus; alpha-L-rhamnosidase; food industry; hydrogen bonding; isoquercitrin; mutagenesis; mutants; pharmaceutical industry; rhamnose; screening; thermal stability;     Show all 12 Subjects
Abstract:
... The α-L-Rhamnosidase is an important enzyme with applications in the food and pharmaceutical industries because it can release terminal L-rhamnose residues from various natural products. In this study, the B-factor-saturation mutagenesis strategy was used to increase the thermostability of α-L-rhamnosidase from Aspergillus terreus. The D594Q and G827K/D594Q mutant enzymes were obtained by screenin ...
DOI:
10.1016/j.jbiotec.2018.03.013

4. Determination and dissipation of afidopyropen and its metabolite in wheat and soil using QuEChERS–UHPLC–MS/MS

Author:
Chen, Yajie; Guo, Mingcheng; Liu, Xingang; Xu, Jun; Dong, Fengshou; Wu, Xiaohu; Li, Baotong; Zheng, Yongquan
Source:
Journal of separation science 2018 v.41 no.7 pp. 8-1681
ISSN:
1615-9306
Subject:
half life,   etc   ; ecosystems; metabolites; models; soil; statistical analysis; tandem mass spectrometry; ultra-performance liquid chromatography; wheat; wheat straw;     Show all 10 Subjects
Abstract:
... The dissipations of afidopyropen and its metabolite in wheat plant and soil were determined using a quick, easy, cheap, effective, rugged, and safe method with ultra‐high performance liquid chromatography and tandem mass spectrometry under a field ecosystem. The limits of quantification were estimated for both target compounds as 0.001 mg/kg. The recoveries of afidopyropen and its metabolite range ...
DOI:
10.1002/jssc.201700773

5. Augmenting atrazine and hexachlorobenzene degradation under different soil redox conditions in a bioelectrochemistry system and an analysis of the relevant microorganisms

Author:
Wang, Hui; Cao, Xian; Li, Lei; Fang, Zhou; Li, Xianning
Source:
Ecotoxicology and environmental safety 2018 v.147 pp. 735-741
ISSN:
0147-6513
Subject:
half life,   etc   ; Desulfitobacterium; Desulfuromonas; Rhodocyclaceae; anodes; atrazine; bioelectrochemistry; hexachlorobenzene; microbial communities; microbial fuel cells; microorganisms; pollutants; soil; sustainable technology;     Show all 14 Subjects
Abstract:
... Soil microbial fuel cells (MFCs) are a sustainable technology that degrades organic pollutants while generating electricity. However, there have been no detailed studies of the mechanisms of pollutant degradation in soil MFCs. In this study, the effects of external resistance and electrode effectiveness on atrazine and hexachlorobenzene (HCB) degradation were evaluated, the performance of soil MFC ...
DOI:
10.1016/j.ecoenv.2017.09.033

6. Fundamental uncertainty equations for nuclear dating applied to the 140Ba-140La and 227Th-223Ra chronometers

Author:
Pommé, S.; Collins, S.M.; Harms, A.; Jerome, S.M.
Source:
Journal of environmental radioactivity 2016 v.162-163 pp. 358-370
ISSN:
0265-931X
Subject:
half life,   etc   ; equations; radionuclides; uncertainty;     Show all 4 Subjects
Abstract:
... Basic equations for age dating through activity ratio measurements are presented and applied to nuclear chronometers based on parent-daughter decay. Uncertainty propagation formulae are derived which relate the relative uncertainty on the half-lives and measured activity ratios with the relative uncertainty on the calculated time of a nuclear event. Particular attention is paid to the case of rela ...
DOI:
10.1016/j.jenvrad.2016.06.013

7. Arylazoindazole Photoswitches: Facile Synthesis and Functionalization via SNAr Substitution

Author:
Travieso-Puente, Raquel; Budzak, Simon; Chen, Juan; Stacko, Peter; Jastrzebski, Johann T. B. H.; Jacquemin, Denis; Otten, Edwin
Source:
Journal of the American Chemical Society 2017 v.139 no.9 pp. 3328-3331
ISSN:
1520-5126
Subject:
half life,   etc   ; ambient temperature; cis-trans isomers; isomerization;     Show all 4 Subjects
Abstract:
... A straightforward synthetic route to arylazoindazoles via nucleophilic aromatic substitution is presented. Upon deprotonation of the NH group, a C₆F₅-substituted formazan undergoes facile cyclization as a result of intermolecular nucleophilic substitution (SNAr). This new class of azo photoswitches containing an indazole five-membered heterocycle shows photochemical isomerization with high fatigue ...
DOI:
10.1021/jacs.6b12585

8. Why Are Vinyl Cations Sluggish Electrophiles?

Author:
Byrne, Peter A.; Kobayashi, Shinjiro; Würthwein, Ernst-Ulrich; Ammer, Johannes; Mayr, Herbert
Source:
Journal of the American Chemical Society 2017 v.139 no.4 pp. 1499-1511
ISSN:
1520-5126
Subject:
half life,   etc   ; bromides; cations; energy; ethanol; ionization; photolysis; pyrroles; solvents;     Show all 9 Subjects
Abstract:
... The kinetics of the reactions of the vinyl cations 2 [Ph₂CC⁺–(4-MeO–C₆H₄)] and 3 [Me₂CC⁺–(4-MeO–C₆H₄)] (generated by laser flash photolysis) with diverse nucleophiles (e.g., pyrroles, halide ions, and solvents containing variable amounts of water or alcohol) have been determined photometrically. It was found that the reactivity order of the nucleophiles toward these vinyl cations is the same as ...
DOI:
10.1021/jacs.6b10889

9. Controlling the Isomerization Rate of an Azo-BF2 Switch Using Aggregation

Author:
Qian, Hai; Wang, Yu-Ying; Guo, Dong-Sheng; Aprahamian, Ivan
Source:
Journal of the American Chemical Society 2017 v.139 no.3 pp. 1037-1040
ISSN:
1520-5126
Subject:
half life,   etc   ; azo compounds; isomerization; photochemistry; reaction mechanisms; synthesis;     Show all 6 Subjects
Abstract:
... A novel visible-light activated azo-BF₂ switch possessing a phenanthridinyl π-system has been synthesized, and its switching properties have been characterized as a function of concentration. The switch self-aggregates through π–π interactions, and the degree of aggregation modulates the Z → E thermal isomerization rate. This property allows for the active tuning of the thermal relaxation half-lif ...
DOI:
10.1021/jacs.6b10982

10. Tuning Azoheteroarene Photoswitch Performance through Heteroaryl Design

Author:
Calbo, Joaquín; Weston, Claire E.; White, Andrew J. P.; Rzepa, Henry S.; Contreras-García, Julia; Fuchter, Matthew J.
Source:
Journal of the American Chemical Society 2017 v.139 no.3 pp. 1261-1274
ISSN:
1520-5126
Subject:
half life,   etc   ; absorption; cis-trans isomers; heterocyclic compounds; irradiation; isomerization;     Show all 6 Subjects
Abstract:
... Photoswitchable compounds, which can be reversibly switched between two isomers by light, continue to attract significant attention for a wide array of applications. Azoheteroarenes represent a relatively new but understudied type of photoswitch, where one of the aryl rings from the conventional azobenzene class has been replaced with a five-membered heteroaromatic ring. Initial studies have sugge ...
DOI:
10.1021/jacs.6b11626

11. Probing Enhanced Double-Strand Break Formation at Abasic Sites within Clustered Lesions in Nucleosome Core Particles

Author:
Banerjee, Samya; Chakraborty, Supratim; Jacinto, Marco Paolo; Paul, Michael D.; Balster, Morgan V.; Greenberg, Marc M.
Source:
Biochemistry 2017 v.56 no.1 pp. 14-21
ISSN:
1520-4995
Subject:
half life,   etc   ; DNA; buffers; histones; nucleosomes; nucleotides; pH; phosphates;     Show all 8 Subjects
Abstract:
... DNA is rapidly cleaved under mild alkaline conditions at apyrimidinic/apurinic sites, but the half-life is several weeks in phosphate buffer (pH 7.5). However, abasic sites are ∼100-fold more reactive within nucleosome core particles (NCPs). Histone proteins catalyze the strand scission, and at superhelical location 1.5, the histone H4 tail is largely responsible for the accelerated cleavage. The ...
DOI:
10.1021/acs.biochem.6b01144

12. Stability of betanin in pitaya powder and confection as affected by resistant maltodextrin

Author:
Shaaruddin, Safura; Mohd Ghazali, Hasanah; Mirhosseini, Seyed Hamed; Muhammad, Kharidah
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2017
ISSN:
0023-6438
Subject:
half life,   etc   ; Cactaceae; juices; maltodextrins; spray drying; sugars; temperature;     Show all 7 Subjects
Abstract:
... Physicochemical properties and stability of betanin in pitaya juice spray dried with maltodextrin (MDp) and resistant maltodextrin (RMDp), and its stability after incorporation into sugar confection were assessed. MDp exhibited more favorable powder properties with higher betanin retention, compared to RMDp. Morphology of MDp exhibited well defined spheres as compared to RMDp which displayed agglo ...
DOI:
10.1016/j.lwt.2017.05.031

13. Quantitative method for analysis of six anticoagulant rodenticides in faeces, applied in a case with repeated samples from a dog

Author:
Seljetun, KristinOpdal; Eliassen, Elin; Karinen, Ritva; Moe, Lars; Vindenes, Vigdis
Source:
Acta veterinaria scandinavica 2018 v.60 no.1 pp. 1-3
ISSN:
1751-0147
Subject:
half life,   etc   ; anticoagulants; blood; brodifacoum; buffers; dogs; feces; formates; ionization; liquid chromatography; liquid-liquid extraction; methanol; monitoring; pH; pharmacokinetics; poisoning; quantitative analysis; tandem mass spectrometry;     Show all 18 Subjects
Abstract:
... BACKGROUND: Accidental poisoning with anticoagulant rodenticides is not uncommon in dogs, but few reports of the elimination kinetics and half-lives in this species have been published. Our objectives were to develop and validate a new method for the quantification of anticoagulant rodenticides in canine blood and faeces using reversed phase ultra-high performance liquid chromatography–tandem mass ...
DOI:
10.1186/s13028-018-0357-9

14. Expression of CLAVATA3 fusions indicates rapid intracellular processing and a role of ERAD

Author:
De Marchis, Francesca; Colanero, Sara; Klein, Eva M.; Mainieri, Davide; Prota, Viviana M.; Bellucci, Michele; Pagliuca, Giampiero; Zironi, Elisa; Gazzotti, Teresa; Vitale, Alessandro; Pompa, Andrea
Source:
Plant science 2018 v.271 pp. 14-80
ISSN:
0168-9452
Subject:
half life,   etc   ; Arabidopsis; amino acids; apical meristems; brefeldin A; endoplasmic reticulum; proteasome inhibitors; protein degradation; protoplasts; root growth; signal peptide; tobacco; transgenic plants;     Show all 13 Subjects
Abstract:
... The 12 amino acid peptide derived from the Arabidopsis soluble secretory protein CLAVATA3 (CLV3) acts at the cell surface in a signalling system that regulates the size of apical meristems. The subcellular pathway involved in releasing the peptide from its precursor is unknown. We show that a CLV3-GFP fusion expressed in transfected tobacco protoplasts or transgenic tobacco plants has very short i ...
DOI:
10.1016/j.plantsci.2018.03.020

15. Determination and pharmacokinetic study of guaiol in rat plasma by gas chromatography–mass spectrometry with selected ion monitoring B Analytical technologies in the biomedical and life sciences

Author:
Yang, Minghan; Luo, Jiaoyang; Li, Kunlun; Hu, Shurong; Ding, Tong; Liu, Hao; Sheng, Ping; Yang, Meihua
Source:
Journal of chromatography 2018 v.1085 pp. 6-35
ISSN:
1570-0232
Subject:
half life,   etc   ; Ferula; chloroform; detection limit; ethyl acetate; gas chromatography-mass spectrometry; ions; liquid-liquid extraction; medicine; monitoring; oral administration; pharmacokinetics; quantitative analysis; rats; statistical analysis;     Show all 15 Subjects
Abstract:
... Guaiol has been used for thousands of years as a traditional Uygur medicine and is the primary active component found in Ferula ferulaeoides (Steud.) Korov (F. ferulaeoides). In our present study, a rapid, selective, and sensitive method of monitoring selected ions was established based on gas chromatography-mass spectrometry. This method was optimized for the quantification and pharmacokinetic an ...
DOI:
10.1016/j.jchromb.2018.03.041

16. Pharmacokinetics and pharmacodynamics of PEGylated truncated human cystathionine beta-synthase for treatment of homocystinuria

Author:
Majtan, Tomas; Bublil, Erez M.; Park, Insun; Arning, Erland; Bottiglieri, Teodoro; Glavin, Frank; Kraus, Jan P.
Source:
Life sciences 2018 v.200 pp. 11-25
ISSN:
0024-3205
Subject:
half life,   etc   ; allometry; blood; clinical trials; cystathionine beta-synthase; dose response; homocysteine; homocystinuria; humans; inflammation; macrophages; metabolites; mice; models; monkeys; pharmacodynamics; pharmacokinetics; rats; therapeutics; tissues; toxicity;     Show all 21 Subjects
Abstract:
... PEGylated human truncated cystathionine beta-synthase, lacking the C-terminal regulatory domain (PEG-CBS), is a promising preclinical candidate for enzyme replacement therapy in homocystinuria (HCU). It was designed to function as a metabolic sink to decrease the severely elevated plasma and tissue homocysteine concentrations. In this communication, we evaluated pharmacokinetics (PK), pharmacodyna ...
DOI:
10.1016/j.lfs.2018.03.018

17. Effect of age on the pharmacokinetics and pharmacodynamics of flunixin meglumine following intravenous and transdermal administration to Holstein calves

Author:
Kleinhenz, Michael D.; Van Engen, Nicholas K.; Gorden, Patrick J.; Smith, Joe S.; KuKanich, Butch; Rajewski, Suzanne M.; Walsh, Philip; Perkins, Steven; Coetzee, Johann F.
Source:
American journal of veterinary research 2018 v.79 no.5 pp. 568-575
ISSN:
0002-9645
Subject:
half life,   etc   ; Holstein; absorption; blood sampling; bulls; calves; enzyme-linked immunosorbent assay; flunixin; intravenous injection; liquid chromatography; mass spectrometry; pharmacodynamics; pharmacokinetics; prostaglandins; veterinary medicine;     Show all 15 Subjects
Abstract:
... OBJECTIVE To determine the effect of age on the pharmacokinetics and pharmacodynamics of flunixin meglumine following IV and transdermal administration to calves. ANIMALS 8 healthy weaned Holstein bull calves. PROCEDURES At 2 months of age, all calves received an injectable solution of flunixin (2.2 mg/kg, IV); then, after a 10-day washout period, calves received a topical formulation of flunixin ...
DOI:
10.2460/ajvr.79.5.568

18. A novel biodegradation pathway of the endocrine-disruptor di(2-ethyl hexyl) phthalate by Pleurotus ostreatus based on quantum chemical investigation

Author:
Ahuactzin-Pérez, Miriam; Tlecuitl-Beristain, Saúl; García-Dávila, Jorge; Santacruz-Juárez, Ericka; González-Pérez, Manuel; Gutiérrez-Ruíz, María Concepción; Sánchez, Carmen
Source:
Ecotoxicology and environmental safety 2018 v.147 pp. 494-499
ISSN:
0147-6513
Subject:
half life,   etc   ; Pleurotus ostreatus; acetic acid; biodegradation; biomass production; butanediol; carbon; energy; gas chromatography-mass spectrometry; glucose; mammals; oxidation; phthalates; phthalic acid; plasticizers;     Show all 15 Subjects
Abstract:
... Di(2-ethyl hexyl) phthalate (DEHP) is a plasticizer that interfere with endocrine systems in mammals. Growth parameters for Pleurotus ostreatus grown on media containing glucose and different concentrations of DEHP (0, 500 and 1000mg/L) were evaluated. The highest biomass production was observed in medium supplemented with 1000mg of DEHP/L. Half-life of DEHP biodegradation, biodegradation constant ...
DOI:
10.1016/j.ecoenv.2017.09.004

19. Reduced graphene oxide enhancing the photoelectrochemical properties of poly(3-hexylthiophene)

Author:
Gonçalves, R.; Moraes, F.C.; Pereira, E.C.
Source:
Carbon 2016 v.109 pp. 57-64
ISSN:
0008-6223
Subject:
half life,   etc   ; carbon; graphene oxide; photocatalysis; polymers;     Show all 5 Subjects
Abstract:
... Poly(3-hexylthiophene) has been used in different photoelectrochemical devices as active material. However, its efficiency regarding photo-conversion is low and the coupling with a second compound is necessary to reduce the electron-hole recombination effect on the generation of photocurrent. The low efficiency could be related to the organization of the polymer. In this work, we propose the use o ...
DOI:
10.1016/j.carbon.2016.07.041

20. Analytical solutions for diffusion of organic contaminant through GCL triple-layer composite liner considering degradation in liner

Author:
Wu, Xun; Shi, Jianyong; He, Jun
Source:
Environmental earth sciences 2016 v.75 no.20 pp. 1371-1371
ISSN:
1866-6280
Subject:
half life,   etc   ; clay; landfills; mass transfer; sorption;     Show all 5 Subjects
Abstract:
... Analytical solutions are presented for analyzing diffusion of organic contaminant through a triple-layer composite liner consisting of a geomembrane, a geosynthetic clay liner (GCL) and an attenuation liner (AL). The degradation of organic contaminant in the GCL and AL is considered. Steady-state and full analytical solutions are obtained for two scenarios with zero-concentration or zero-mass-flux ...
DOI:
10.1007/s12665-016-6145-9